140 results about "Coenzyme I" patented technology

The invention discloses artificial bear gall powder and a preparation method thereof. The preparation method comprises the following steps: step (a) dissolving livestock gall powder by using a buffer solution of which the pH value is equal to 7-9 and then filtering to obtain filter residues and a filtrate; step (b) adding the filtrate into a reactor filled with 7alpha-hydroxyl steroid dehydrogenase, 7beta-hydroxyl steroid dehydrogenase and coenzyme I or coenzyme II and reacting; step (c) after the reaction ends, freeze-drying a reaction liquid and then mixing the freeze-dried reaction liquid with the filter residues to obtain the artificial bear gall powder. According to the preparation method disclosed by the invention, completely natural livestock gall is taken as a raw material for preparing the artificial bear gall, no other chemical substances are added, common animal gall is adopted and a gall source is easy to pick, obtain and process; through an in-vitro enterohepatic circulation simulation process, the livestock gall is biologically transformed into the artificial bear gall powder which is equivalent to natural bear gall powder in chemistry, and the artificial bear gall powder is used as a natural bear gall replacement to protect the endangered animal Asian black bear and promote healthy development of the bear gall industry.

The invention discloses a 7beta-hydroxyl sterol dehydrogenase mutant which is obtained by protein engineering and of which coenzyme preference is changed, a coding gene of the 7beta-hydroxyl sterol dehydrogenase mutant, a recombinant expression vector and a recombinant expression transformant which contain a sequence of the gene, a preparation method of a recombinant mutant enzyme preparation, andapplication of the recombinant mutant enzyme preparation to preparation of ursodeoxycholic acid. By co-enzyme regeneration of enzymic coupling, the recombinant mutant enzyme preparation disclosed bythe invention can efficiently utilize relatively cheap oxidized coenzyme I (NAD+) instead of very expensive oxidized coenzyme II (NADP+); asymmetric reduction of catalytic 7-hydroxyl lithocholic acideffectively reduces production cost; moreover, the recombinant mutant enzyme preparation has the advantages of simplicity for operation, mild reaction condition, environmental-friendliness, high yieldand the like, and has a good application prospect in preparation of ursodeoxycholic acid by epimerization of chenodesoxycholic acid.

The invention provides a stable detection kit of total bile acid. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components: a buffering solution, beta-thionicotinamide adenine dinucleotide, a surface active agent, a stabilizing agent and a preservative; the reagent R2 comprises the following components: a buffering solution, reduction type coenzyme I (NADH), 3alpha-hydroxysteroid, a surface active agent, antioxidant enzyme, a stabilizing agent and a preservative. The stable detection kit provided by the invention has the advantages that since the antioxidant enzyme is added into the reagents, NADH can be stored for a long time, the influence caused by more interference components in the reaction process is eliminated, so that the NADH can be utilized efficiently, the speed of circulating reaction is increased, the detection accuracy is improved, the sensitivity is high, the linear range is wide, simultaneously, the used amount of main reaction materials can be reduced and the cost of the reagents is reduced.

The invention provides a reagent for quantitatively determining content of total bile acid in a human serum sample through an enzymological method. The reagent is suitable for an automatic biochemical analyzer to automatically and quantitatively determine the total bile acid. The reagent consists of a solution reagent 1 and a reagent 2 which are separately placed, wherein the reagent 1 contains oxidized thio-nicotinamide-adenine dinucleotide (Thio-NAD), buffer and stabilizer; and the reagent 2 contains reduced coenzyme I (NADH), hydroxysteroiddehydrogenase (HSD), stabilizer and buffer. The invention additionally provides a reagent kit with the reagent 1 and the reagent 2, and a method for determining the content of total bile acid in the serum. The reagent, the reagent kit and the method provided by the invention have the advantages of high sensitivity, low cost, simplicity, convenience and rapidness in operation and easiness in popularization.

The invention discloses a liquid stable kit for measuring beta-hydroxybutyric acid by a cyclic enzyme method. The liquid stable kit consists of a reagent 1 and a reagent 2, wherein 1L of reagent 1 comprises 50 to 500mmol of buffer solution, 1 to 5KU of beta-hydroxybutyrate dehydrogenase, 1 to 5KU of diaphorase, 0.1 to 100g of surfactant, 1 to 100mmol of stabilizer, 0.1 to 100g of anti-interference agent I, 0.1 to 100g of anti-interference agent II, and 0.1 to 100ml of preservative; and 1L of reagent 2 comprises 50 to 500mmol of buffer solution, 1 to 20mmol of coenzyme I, 0.1 to 10mmol of nitrotetrazolium blue, 1 to 100mmol of stabilizer and 0.1 to 100ml of preservative. The liquid stable kit has the advantages of stability, wide linear range, high measurement accuracy, high antijamming capability and low cost.

The invention discloses a creatine jubase detection reagent. The reagent disclosed by the invention consists of a reagent R1 and a reagent R2 according to a volume ratio being 4 to 1, wherein the reagent R1 consists of an imidazole buffer solution, glucose, nano particles, N-acetylcysteine, ethylenediaminetetraacetic acid disodium salt, adenosine diphosphate, coenzyme I, adenine ribonucleotide, pyruvate decarboxylase, 6-phosphogluconic dehydrogenase, hexokinase and sodium dodecyl benzene sulphonate; and the reagent R2 consists of an imidazole buffer solution, phosphocreatine, a preservative and sodium dodecyl benzene sulphonate. Pyruvate decarboxylase and gamma-Fe2O3 nano particles are added into the creatine jubase detection reagent disclosed by the invention, so that interfering substances in a serum sample can be effectively eliminated, metal ions in the serum sample are chelated by matching sodium dodecyl benzene sulphonate and ethylenediaminetetraacetic acid disodium salt, the influence on reaction enzymes is alleviated, and the reagent is a stable, accurate and sensitive detection reagent with high interference resistance.

The invention relates to a preparation method of an oxidation coenzyme I. According to the method, nicotinamide ribonucleoside (NR) and adenosine disodium triphosphate (ATP-Na2) react with each other to obtain the oxidation coenzyme I (NAD+, Nicotinamide Adenine Dinucleotide+) in a buffer solution with the pH of 5.0 to 8.0 at a temperature of 30 DEG C to 40 DEG C under the catalytic action of nicotinamide ribonucleoside kinase (NRK) under the condition of the existence of divalent metal ions. According to the invention, a biocatalysis method is adopted, the oxidation coenzyme is prepared by utilizing an NRK one pot process, the reaction system is simple, the conditions are mild, and the preparation method has wide industrialization application prospect.

The invention relates to the field of medicaments and discloses an antialcoholismic composition. The raw materials of the antialcoholismic composition comprise: one or a mixture of more than two of S-adenosyl methionine, cysteine and methionine, one of a mixture of D-glyceric acid and D-glycerate, one or a mixture of more than two of succinic acid, fumaric acid, citric acid, oxaloacetic acid and malic acid, one or a mixture of more than two of vitamin B1, vitamin B6 and vitamin B2, one or a mixture of coenzyme Q10 or coenzyme I, L-glutamine, fortune eupatorium herb, artemisia capillaries, white paeony root and root of tall monkshood. The invention also provides a preparation method of the antialcoholismic composition. The antialcoholismic composition disclosed by the invention is taken after wine drinking for reducing absorption of alcohol, accelerating alcohol metabolism, as well as tonifying stomach and spleen and preventing drunkenness and alcoholism. The antialcoholismic composition is quick in effectiveness and has a bright application prospect.

The invention discloses an anti-aging composition for improving co-enzyme I, a preparation and a preparation method thereof. The anti-aging composition is prepared from the following raw materials andcomponents in parts by weight: 0.005 to 0.02 part of methylpyridine chromium, 0.05 to 0.2 part of folic acid, 100 to 250 parts of quercetin, 30 to 80 parts of resveratrol, 10 to 100 parts of niacinamide, 10 to 300 parts of niacin, 10 to 50 parts of soluble magnesium, and 300 to 600 parts of leucine. The anti-aging composition and the preparation have the advantages that the content of the coenzyme I (NAD) in cells is effectively increased; by compounding with other natural components, the histone deacetylase family (SIRT 1-7) for cell growth, metabolism and survival can be more effectively activated, the health of mitochondria is maintained, the energy metabolism of the cells is optimized, the activity of the gene repair enzyme PARP1 is maintained, the injury of the cells is restored, thegeneration of the cells is enhanced, and the functions of a human body are regulated and optimized; a user can reach the purposes of more effectively resisting aging, and reducing deteriorative disease risk of the patient.

The invention provides a total bile acid measuring kit. The kit comprises a reagent A and a reagent B, wherein the reagent A comprises Thio-NAD, a buffer solution A, a surface active agent and a stabilizing agent A; the reagent B comprises 3 alpha-HSD, reduced coenzyme I, a surface active agent, a buffer solution B and a stabilizing agent B. Through the technical scheme, when the total bile acid measuring kit is in an uncovered state for use at the temperature ranging from 2 DEG C to 8 DEG C, the stabilization time can reach 20-30 days, and the stabilization time in the airtight and light shielding storage process at the temperature ranging from 2 DEG C to 8 DEG C can reach 10-15 months.

The invention discloses a preparation method of oxidizing-type coenzyme I, which includes the steps of disrupting yeast cells and extracting NAD+. The step of disrupting yeast cells particularly comprises: (1) soaking yeast in hydrochloric acid, heating the yeast, maintaining the temperature, adding ice cubes to cool the liquid to room temperature, and performing centrifugation to obtain a clear solution A; and (2) stirring the clear solution A for 12-16 h with addition of 717# resin, and performing filtration after stirring to obtain a clear solution B. The step of extracting NAD+ particularly comprises the following steps: acidification, exchange, elution, desalination, separation, elution and collection. The preparation method greatly increases disruption rate of yeast cells. The raw materials are low in cost and are easy to obtain. The device is simple and is convenient to use. The preparation method is suitable for industrial production.

The invention relates to the technical field of detection for GLU in the blood by adopting hexokinase, and in particular relates to a detection reagent for GLU in the blood, which is strong in stability and low in cost and adopts liquid hexokinase. The detection reagent adopts liquid double reagents, wherein the reagent I mainly contains a buffer solution, Mg<2+>, an ion balancing agent, a preservative, NAD (oxidized coenzyme I), a protective agent and a surfactant, and the reagent II contains a buffer solution, a protective agent, a surfactant, preservative, hexokinase (HK), glucose-6-phosphate dehydrogenase (G6P-DH), ATP.NA2, and the like. In order to guarantee the stability of enzymes in the reagent, various protective agents including polyethylene glycol-20000, tween-80, FAD and the like are added in the reagent in a pertinence manner, in order to reduce the cost, especially the use of enzymes, various surfactants are added in the reagent, so that the emulsifying for enzymes is enhanced, the service efficiency for enzymes is improved, thus the cost of the reagent is greatly reduced, and the detection reagent is particularly suitable for being used and popularized in clinic.

The invention discloses reaction liquid for preparing dry chemical test paper for determination of alpha-hydroxybutyrate dehydrogenase, and dry chemical test paper. The dry chemical test paper comprises a reaction chromogenic layer containing alpha-hydroxybutyric acid, coenzyme I, diaphorase and a chromogenic reagent capable of generating a chromogenic reaction with NADH under the catalysis of diaphorase. The dry chemical test paper prepared by the reaction liquid can be used in combination with a small instrument to determine the content of alpha-hydroxybutyrate dehydrogenase in a short period of time only by collecting a small amount of blood, the operation is simple and convenient, no professional operation is required, the intensity of color development is high, the uniformity is good,the pollution is small, the detection process does not rely on a large-scale biochemical analyzer, the market requirements under special conditions can be met, especially emergency treatment, and compared with a traditional method for detecting by adopting a liquid biochemical reagent, the dry chemical test paper can provide a method capable of conveniently and quickly measuring the activity of alpha-hydroxybutyrate dehydrogenase for the emergency department, primary hospitals, families and small clinics.

The invention provides a liquid medium used for culturing haemophilus parasuis. The liquid medium comprises the following components: tryptone, soya peptone, sodium chloride, dipotassium phosphate, glucose, yeast powder, bovine serum and a coenzyme I, and the liquid medium has a pH value of 7.2-7.4. The liquid medium has the beneficial effects that the liquid medium is beneficial to growth of haemophilus parasuis by supplementing the nutritional ingredients required by various microorganisms and effectively controlling the pH value in the medium via sodium hydroxide; the viable counts are not lower than 10<9>CFU / ml; and inactivated vaccines are prepared from haemophilus parasuis liquid and have good safety and immune efficacy.

The invention provides a method for measuring beta-hydroxybutyric acid by using enzyme colorimetric reaction, a matched kit and application thereof. Under the system of Tris-HCL buffer solution, serum beta-hydroxybutyric acid and coenzyme I are dehydrogenized under the catalysis of beta-hydroxybutyric acid dehydrogenase to generate acetoacetic acid and reduced coenzyme I, the generated reduced coenzyme I and iodonitrotetrazole chloride are reacted under the catalysis of diaphorase to generate a red substance formazane of which the highest absorbance is 505 nanometers, and then the content of the beta-hydroxybutyric acid in a biological sample is quantified by measuring the change of the absorbance of the red product at the wavelength of 505 nanometers. The method can linearly measure the concentration range (0 to 4.5 mmol / L) of the beta-hydroxybutyric acid in the biological sample, the measurement is used for judging the human ketosis for acid poisoning diagnosis, the reagent used by the method is liquid dual-reagent, and the quantity of the sample required for measuring the beta-hydroxybutyric acid is little; moreover, the reaction time during measuring is short, the operation is simple, and the method is suitable for mass detection.

The invention discloses a kit for testing the genetic risk of esophageal cancer, which comprises specific premier pairs for testing the single nucleotide polymorphic locus genotypes of a cytochrome P450 1A1 gene (CYP1A1), a cytochrome P450 2E1 gene (CYP2E1), a glutathione-S-transferase M1 gene (GSTM1), a 5,10-methylenetetrahydrofo late reductase gene (MTHFR), a reduction type coenzyme I quinone oxido-reductase gene (NQ01), an oncoprotein 53 gene (P53), an X-ray repair cross-complementing gene 1 (XRCC1) and a xeroderma pigmentosum complementary set A gene (XPA) as well as specific fluorescent probe pairs, fluorescent quantitative PCR regular components, PCR reaction components and the like. The kit of the invention estimates the individual genetic risk of esophageal cancer by synchronously testing the polymorphic locus genotypes of the genes closely related to the genetic risk of esophageal cancer.

The invention discloses an aspartate transaminase mitochondrial isozyme detection kit, comprising: reagents 1: 0.02-0.5mol / L of buffer solution of which pH value is 6.5-8.0, 0.15-0.3mol / L of L-potassium aspartate, 0.3-5KU / L of malic dehydrogenase, 0.3-5KU / L of lactic dehydrogenase, 5-20mL / L of human cAST antibody, 0.01-3% of antibody stabilizer, 0.01-10% of enzyme stabilizer and 0.01-1% of preservative; and reagents 2: 0.02-0.5mol / L of buffer solution of which pH value is 7.0-9.0, 0.005-0.02mol / L of alpha-ketoglutarate, 0.1-0.5mmol / L of reduced coenzyme I, 0.01-1% of preservative and 0.01-10% of reduced coenzyme I stabilizer. The kit has the advantage of good stability.

The invention discloses a mesenchymal stem cell culture medium. The mesenchymal stem cell culture medium is prepared from a DMEM, hydroxyethyl starch, human serum albumin, potassium chloride, sodium selenite, transferrin, glutathione, fibroblast growth factors, human insulin-like growth factors, coenzyme I, lipoic acid, vitamins, antibiotic, astragalus polysaccharide, polygonatum polysaccharide and Chinese date extract. According to the mesenchymal stem cell culture medium, residues or pollution will not be caused as no animal source ingredient is contained, the cell wall attachment performance is good, the proliferation speed is high, the anti-pathogenic microorganism effect of the culture medium is improved, and meanwhile the cost of the culture medium is reduced.

The invention provides a stable compound coenzyme preparation and a preparation method thereof. The main compositions and proportions of the stable compound coenzyme preparation are as follows: 90-120 U of coenzyme A (CoA), 0.1-0.3 mg / ml coenzyme I (NAD), 1-5 mg / ml glutathione (GSH), 1-2.5 mg / ml adenosine triphosphate (ATP), 1.8-12 mu g / ml flavin mononucleotide (FMN), 3-13 mu g / ml flavin adenine dinucleotide (FAD), 0.1-0.4 mg / ml adenosine diphosphate (ADP), 0.2-0.4 mg / ml adenosine monophosphate (AMP), 1.2-15 mu g / ml of adenosine methionine (SAM), 1-5 mg / ml calcium gluconate, 0.5-1.5 mg / ml cysteine hydrochloride, and 0.6-5 mg / ml mannitol. The preparation method overcomes the defects that high-speed centrifugalization is adopted, freeze-drying conditions are not clear and the product stability is poor in the existing method, and the stable compound coenzyme preparation is widely applied to the practices of preparing drugs for treating cardia-cerebrovascular diseases and digestive system diseases.

The invention relates to a homocysteine concentration measuring method. The method includes: oxidizing homocysteine to generate alpha-butanone acid, ammonia and hydrogen sulfide; using ammonia to convert NADH into NAD+ under action of glutamate dehydrogenase; quantifying by measuring light absorbance of NADH at a position of 340nm. The homocysteine diagnosis kit comprises a reagent I and a reagent II, the reagent I contains reduced coenzyme I, glutamate dehydrogenase, tri(2-carboxyethyl) phosphine hydrochloride, alpha-ketoglutaric acid, surfactant, buffer solution and stabilizer, and the reagent II contains HCYase, phosphopyridoxal, buffer solution and stabilizer. The homocysteine diagnosis kit and the homocysteine concentration measuring method have the advantages that the kit for detecting homocysteine in human serum through two liquid reagents is built, and an additional fluorescence detector is not needed, so that convenience is brought to detection of plenty of samples; the method is free of interference by pyruvic acid, methionine and cysteine in the samples and lower in cost.

The invention discloses a multifunctional skin care product with effects of whitening skin, removing freckles, preserving moisture and removing wrinkle and a preparation method of the multifunctional skin care product. The skin care product is prepared from food-grade or medical-grade skin nutrient composition, skin protection composition and a solvent, contains multiple nutrients such as hyaluronic acid, collagen, vitamins, amino acid and the like required by normal skin nutrition, and also contains healthcare components such as coenzyme I, coenzyme II, nicotinamide, coenzyme Q10 and the like required by skin health maintenance; the skin care product has remarkable effects of whitening skin, preserving moisture, removing wrinkle and removing freckles, and is anti-aging and anti-sunburn; the skin care product has the advantages of high safety, wide applicable range, good effects and the like, and is convenient to use, non-irritant, non-allergic and suitable for almost all people; all common skin abnormality problems can be solved with the product, besides, the skin care product takes effect rapidly, and can be used for a long time without producing toxic and side effects; the preparation method of the skin care product adopts mild conditions and a simple process, does not cause environmental pollution and is worthy of large-scale popularization.

The present invention provides a pyruvic acid determination reagent box. The reagent box comprises a reagent R1 and a reagent R2. The reagent R1 comprises the following ingredients: reduced coenzyme I(NADH), a trihydroxymethyl aminomethane (Tris) buffer solution, a surface active agent, EDTA-Na2 and a preservative. The reagent R2 comprises the following ingredients: the trihydroxymethyl aminomethane (Tris) buffer solution, lactate dehydrogenase (LDH), the surface active agent, a stabilizer and the preservative. The present also provides a preparation method of the reagent box and applications. The reagent box is a high-stability, high-accuracy and good-repeatability liquid reagent box.

The invention discloses bread yeast and an application thereof in producing a coenzyme I by fermenting. The bread yeast is prepared through the steps of after original starting strains sc-00 are mutated by implanting a low-energy nitrogen ion beam, carrying out preliminary screening on the strains, fermenting in a shake flask, and carrying out secondary screening; and the bread yeast is classified and named Saccharomycescerevisiae SC-Z180, and preserved in China Center for Type Culture Collection CCTCC, and the Preservation Number is CCTCCM2014317. The bread yeast can be used for greatly increasing the components of the coenzyme I in the process of fermentation, and reducing other components; and through carrying out fermentation and secondary screening on obtained strains, the strains can be used for significantly improving the problem that the fermentation yield is low, and in a fermentation tank with a volume of 5 L, the yield of the coenzyme I is increased by 45.8% in comparison with that of original starting strains.

The invention discloses a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method for identifying seven sea cucumber species. The PCR-RFLP method includes the steps of firstly extracting DNA (deoxyribonucleic acid) modules of sea cucumbers; subjecting specificity fragment of a CO I (coenzyme I) gene to PCR amplification to obtain a fragment about 690bp; secondarily subjecting amplified product to restriction enzyme digestion; and finally subjecting the enzyme-digested product to electrophoresis detection. Different sea cucumbers obtain stripes in different numbers and sizes, and selected specificity incision enzymes respectively include: Californian paracanthurus cucumber: SSP I, North Atlantic Ocean cucumaria: EcoR V, apostichopus japonicas: SSP I, thelenota ananas: EcoR V, acaudina molpadioidea: Sca I, isostichopusbadionotus: Pvu II, and actinopyga mauritiana: EcoR V and SSP I. The PCR-RFLP method overcomes the defects that a conventional morphological identification method is prone to affecting in accuracy, is heavy in workload and long in consumption time and the like. The PCR-RFLP method can be completed by only one-time PCR reaction, one-time enzyme digestion reaction and one-time polypropylene gel electrophoresis, and is simple to operate, short in parting time and good in specificity.

The invention relates to a method for preparing L-2-aminobutyric acid through whole-cell bioconversion. The method disclosed by the invention comprises the following steps of: carrying out tandem expression on threonine deaminase and leucine dehydrogenase, and cloning on a chlorampenicol resistant expression vector so as to realize co-expression of the two enzymes in the same bacterium; cloning formilase on a kalamycin resistant pET-28a vector to realize expression, and carrying out tandem expression with a pcnB gene in a rate-limiting step of coenzyme I metabolic pathways in escherichia coli so as to realize high expression of the two enzymes; converting the expression vector having two resistances into the same bacterium to carry out co-expression so as to realize expression of four enzymes in the same host bacterium; and carrying out fermentation expression of whole-cell for escherichia coli containing the four enzymes by using escherichia coli so as to realize biological reduction conversion from threonine to L-2-aminobutyric acid. According to the invention, high-efficiency conversion of 2-ketobutyric acid can be realized without addition of coenzyme; the cost is low; and the method is simple.

The invention discloses a multilayer-film dry chemical reagent strip for measuring glutamic-pyruvic transaminase by using enzyme coupled continuous monitoring assay. The reagent strip comprises four layers of film, namely diffusion film, blood filter film (in two layers) and bottom reaction film and is characterized in that corresponding enzyme reactants are adsorbed to the reaction film, pyruvic acid is generated from L-alanine and sodium Alpha-ketoglutarate under the catalysis of glutamic-pyruvic transaminase, the pyruvic acid acts with reduced coenzyme I(NADH) under the catalysis of lactic dehydrogenase (LDH) to generate NAD+, absorbance at 340 nm is decreased, and the amplitude of decrease is associated with the content of the glutamic-pyruvic transaminase. The reagent strip is applicable to the quick measurement of the activity of glutamic-pyruvic transaminase in human whole blood and serum or plasma, is simple and easy to operate, and is easy to clinically apply and popularize.

The invention discloses a kit for detecting homocysteine. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains the following components and concentrations: 10-200mmol / L of buffer solution, 1-10mmol / L of homocysteine reducing agent, 0.1-15mmol / L of serine, 0.2-5.8mg / L of coenzyme, 5-100KU / L of lactic dehydrogenase, 0.01-0.8g / L of reducing coenzyme I, 5-150g / L of enzyme stabilizer, 1-100g / L of surface active agent and 0.1-5g / L of preservative; the reagent R2 contains the following components and concentrations: 10-200mmol / l of buffer solution, 0.2-5.8mg / L of coenzyme, 1-100KU / L of cystathionine beta-synthetase, 1-100KU / L of cystathionine beta-lyase, 5-150g / L of enzyme stabilizer, 1-100g / L of surface active agent and 0.1-5g / L of preservative. The kit disclosed by the invention has the advantages that the use amount of enzyme can be reduced, the stability of detection can be improved, the sensitivity of cyclic detection can be improved, the accuracy is high and the application range is wide. The method is good in correlation with an HPLC (High Performance Liquid Chromatography) method, pretreatment for a sample is not needed, the detection on a full-automatic biochemical analyzer is automatic and the speed is high.

The invention discloses an acne-removing, mark-eliminating and skin-protecting multifunctional preparation with both acne-removing and mark-eliminating effects and skin-beautifying and skin-protecting effects and a preparation method thereof. The acne-removing, mark-eliminating and skin-protecting multifunctional preparation is composed of a skin care composition, an acne-removing and mark-eliminating composition and a solvent, and not only contains collagen, vitamins and other various nutrients required for normal skin nutrition, but also contains a coenzyme I, a coenzyme II, nicotinamide, a coenzyme Q10 and other healthcare components required for skin health maintenance. The acne-removing, mark-eliminating and skin-protecting multifunctional preparation not only has disinfection, anticorrosion and anti-bacterium components, but also has injured tissue repair components, and the effective combination of the multiple components can generate mild acne-removing and mark-eliminating effects; the acne-removing, mark-eliminating and skin-protecting multifunctional preparation has the advantages of being high in safety, convenient to use, wide in application range, excellent in effect and the like. The acne-removing, mark-eliminating and skin-protecting multifunctional preparation disclosed by the invention is non-toxic, free from irritation and allergy and almost suitable for all people. The preparation method of the acne-removing, mark-eliminating and skin-protecting multifunctional preparation, disclosed by the invention, has the advantages of mild condition, no need of temperature rise, low energy consumption, simple and short process flow and no environmental pollution, and is a green manufacturing technology that is worth of being popularized.

The invention discloses a culture medium for rapid proliferation of neural stem cells. The culture medium is prepared from an RPMI-1640 culture medium, trehalose, recombinant human insulin, insulin growth factors, endothelial cell growth factors, potassium chloride, amino acid chelated selenium, glutamine, progesterone, coenzyme I, lipoic acid, vitamin, polygahatous polysaccharides, polysaccharide from radix codonopsis and ginseng saponin. The culture medium for rapid proliferation of the neural stem cells is good in cell wall adherence, fast in proliferation and free of animal source ingredients and does not produce residues or pollution, meanwhile the anti-pathogen microbial effect of the culture medium can be improved, and the cost of the culture medium can be reduced.