140 results about "Coenzyme I" patented technology

The invention discloses a 7beta-hydroxyl sterol dehydrogenase mutant which is obtained by protein engineering and of which coenzyme preference is changed, a coding gene of the 7beta-hydroxyl sterol dehydrogenase mutant, a recombinant expression vector and a recombinant expression transformant which contain a sequence of the gene, a preparation method of a recombinant mutant enzyme preparation, andapplication of the recombinant mutant enzyme preparation to preparation of ursodeoxycholic acid. By co-enzyme regeneration of enzymic coupling, the recombinant mutant enzyme preparation disclosed bythe invention can efficiently utilize relatively cheap oxidized coenzyme I (NAD+) instead of very expensive oxidized coenzyme II (NADP+); asymmetric reduction of catalytic 7-hydroxyl lithocholic acideffectively reduces production cost; moreover, the recombinant mutant enzyme preparation has the advantages of simplicity for operation, mild reaction condition, environmental-friendliness, high yieldand the like, and has a good application prospect in preparation of ursodeoxycholic acid by epimerization of chenodesoxycholic acid.

The invention provides a reagent for quantitatively determining content of total bile acid in a human serum sample through an enzymological method. The reagent is suitable for an automatic biochemical analyzer to automatically and quantitatively determine the total bile acid. The reagent consists of a solution reagent 1 and a reagent 2 which are separately placed, wherein the reagent 1 contains oxidized thio-nicotinamide-adenine dinucleotide (Thio-NAD), buffer and stabilizer; and the reagent 2 contains reduced coenzyme I (NADH), hydroxysteroiddehydrogenase (HSD), stabilizer and buffer. The invention additionally provides a reagent kit with the reagent 1 and the reagent 2, and a method for determining the content of total bile acid in the serum. The reagent, the reagent kit and the method provided by the invention have the advantages of high sensitivity, low cost, simplicity, convenience and rapidness in operation and easiness in popularization.

The invention discloses a liquid stable kit for measuring beta-hydroxybutyric acid by a cyclic enzyme method. The liquid stable kit consists of a reagent 1 and a reagent 2, wherein 1L of reagent 1 comprises 50 to 500mmol of buffer solution, 1 to 5KU of beta-hydroxybutyrate dehydrogenase, 1 to 5KU of diaphorase, 0.1 to 100g of surfactant, 1 to 100mmol of stabilizer, 0.1 to 100g of anti-interference agent I, 0.1 to 100g of anti-interference agent II, and 0.1 to 100ml of preservative; and 1L of reagent 2 comprises 50 to 500mmol of buffer solution, 1 to 20mmol of coenzyme I, 0.1 to 10mmol of nitrotetrazolium blue, 1 to 100mmol of stabilizer and 0.1 to 100ml of preservative. The liquid stable kit has the advantages of stability, wide linear range, high measurement accuracy, high antijamming capability and low cost.

The invention discloses a creatine jubase detection reagent. The reagent disclosed by the invention consists of a reagent R1 and a reagent R2 according to a volume ratio being 4 to 1, wherein the reagent R1 consists of an imidazole buffer solution, glucose, nano particles, N-acetylcysteine, ethylenediaminetetraacetic acid disodium salt, adenosine diphosphate, coenzyme I, adenine ribonucleotide, pyruvate decarboxylase, 6-phosphogluconic dehydrogenase, hexokinase and sodium dodecyl benzene sulphonate; and the reagent R2 consists of an imidazole buffer solution, phosphocreatine, a preservative and sodium dodecyl benzene sulphonate. Pyruvate decarboxylase and gamma-Fe2O3 nano particles are added into the creatine jubase detection reagent disclosed by the invention, so that interfering substances in a serum sample can be effectively eliminated, metal ions in the serum sample are chelated by matching sodium dodecyl benzene sulphonate and ethylenediaminetetraacetic acid disodium salt, the influence on reaction enzymes is alleviated, and the reagent is a stable, accurate and sensitive detection reagent with high interference resistance.

The invention relates to the field of medicaments and discloses an antialcoholismic composition. The raw materials of the antialcoholismic composition comprise: one or a mixture of more than two of S-adenosyl methionine, cysteine and methionine, one of a mixture of D-glyceric acid and D-glycerate, one or a mixture of more than two of succinic acid, fumaric acid, citric acid, oxaloacetic acid and malic acid, one or a mixture of more than two of vitamin B1, vitamin B6 and vitamin B2, one or a mixture of coenzyme Q10 or coenzyme I, L-glutamine, fortune eupatorium herb, artemisia capillaries, white paeony root and root of tall monkshood. The invention also provides a preparation method of the antialcoholismic composition. The antialcoholismic composition disclosed by the invention is taken after wine drinking for reducing absorption of alcohol, accelerating alcohol metabolism, as well as tonifying stomach and spleen and preventing drunkenness and alcoholism. The antialcoholismic composition is quick in effectiveness and has a bright application prospect.

The invention discloses reaction liquid for preparing dry chemical test paper for determination of alpha-hydroxybutyrate dehydrogenase, and dry chemical test paper. The dry chemical test paper comprises a reaction chromogenic layer containing alpha-hydroxybutyric acid, coenzyme I, diaphorase and a chromogenic reagent capable of generating a chromogenic reaction with NADH under the catalysis of diaphorase. The dry chemical test paper prepared by the reaction liquid can be used in combination with a small instrument to determine the content of alpha-hydroxybutyrate dehydrogenase in a short period of time only by collecting a small amount of blood, the operation is simple and convenient, no professional operation is required, the intensity of color development is high, the uniformity is good,the pollution is small, the detection process does not rely on a large-scale biochemical analyzer, the market requirements under special conditions can be met, especially emergency treatment, and compared with a traditional method for detecting by adopting a liquid biochemical reagent, the dry chemical test paper can provide a method capable of conveniently and quickly measuring the activity of alpha-hydroxybutyrate dehydrogenase for the emergency department, primary hospitals, families and small clinics.

The invention provides a liquid medium used for culturing haemophilus parasuis. The liquid medium comprises the following components: tryptone, soya peptone, sodium chloride, dipotassium phosphate, glucose, yeast powder, bovine serum and a coenzyme I, and the liquid medium has a pH value of 7.2-7.4. The liquid medium has the beneficial effects that the liquid medium is beneficial to growth of haemophilus parasuis by supplementing the nutritional ingredients required by various microorganisms and effectively controlling the pH value in the medium via sodium hydroxide; the viable counts are not lower than 10<9>CFU / ml; and inactivated vaccines are prepared from haemophilus parasuis liquid and have good safety and immune efficacy.

The invention provides a method for measuring beta-hydroxybutyric acid by using enzyme colorimetric reaction, a matched kit and application thereof. Under the system of Tris-HCL buffer solution, serum beta-hydroxybutyric acid and coenzyme I are dehydrogenized under the catalysis of beta-hydroxybutyric acid dehydrogenase to generate acetoacetic acid and reduced coenzyme I, the generated reduced coenzyme I and iodonitrotetrazole chloride are reacted under the catalysis of diaphorase to generate a red substance formazane of which the highest absorbance is 505 nanometers, and then the content of the beta-hydroxybutyric acid in a biological sample is quantified by measuring the change of the absorbance of the red product at the wavelength of 505 nanometers. The method can linearly measure the concentration range (0 to 4.5 mmol / L) of the beta-hydroxybutyric acid in the biological sample, the measurement is used for judging the human ketosis for acid poisoning diagnosis, the reagent used by the method is liquid dual-reagent, and the quantity of the sample required for measuring the beta-hydroxybutyric acid is little; moreover, the reaction time during measuring is short, the operation is simple, and the method is suitable for mass detection.

The invention discloses an artificial bear bile powder and a preparation method thereof. The preparation method comprises the following steps: a) dissolving poultry bile powder with a buffer solution of pH=7-9 and then filtering to obtain filter residue and filtrate; b ) adding the filtrate to a reactor equipped with 7α-hydroxysteroid dehydrogenase, 7β-hydroxysteroid dehydrogenase and coenzyme I or coenzyme II for reaction; c) freeze-drying the reaction solution after the reaction and combining with the The filter residue is mixed to obtain artificial bear bile powder. The present invention completely uses natural sources of poultry bile as the raw material for artificial bear bile, without adding other chemical substances, and adopts common animal bile, and its bile source is easy to collect, obtain, and process; through in vitro simulation of the enterohepatic circulation process , to bioconvert poultry bile into artificial bear bile powder that is chemically equivalent to natural bear bile powder, as a substitute for natural bear bile, to protect the endangered Asian black bear, and to promote the healthy development of the bear bile industry.

The invention provides a stable compound coenzyme preparation and a preparation method thereof. The main compositions and proportions of the stable compound coenzyme preparation are as follows: 90-120 U of coenzyme A (CoA), 0.1-0.3 mg / ml coenzyme I (NAD), 1-5 mg / ml glutathione (GSH), 1-2.5 mg / ml adenosine triphosphate (ATP), 1.8-12 mu g / ml flavin mononucleotide (FMN), 3-13 mu g / ml flavin adenine dinucleotide (FAD), 0.1-0.4 mg / ml adenosine diphosphate (ADP), 0.2-0.4 mg / ml adenosine monophosphate (AMP), 1.2-15 mu g / ml of adenosine methionine (SAM), 1-5 mg / ml calcium gluconate, 0.5-1.5 mg / ml cysteine hydrochloride, and 0.6-5 mg / ml mannitol. The preparation method overcomes the defects that high-speed centrifugalization is adopted, freeze-drying conditions are not clear and the product stability is poor in the existing method, and the stable compound coenzyme preparation is widely applied to the practices of preparing drugs for treating cardia-cerebrovascular diseases and digestive system diseases.

The invention discloses a multifunctional skin care product with effects of whitening skin, removing freckles, preserving moisture and removing wrinkle and a preparation method of the multifunctional skin care product. The skin care product is prepared from food-grade or medical-grade skin nutrient composition, skin protection composition and a solvent, contains multiple nutrients such as hyaluronic acid, collagen, vitamins, amino acid and the like required by normal skin nutrition, and also contains healthcare components such as coenzyme I, coenzyme II, nicotinamide, coenzyme Q10 and the like required by skin health maintenance; the skin care product has remarkable effects of whitening skin, preserving moisture, removing wrinkle and removing freckles, and is anti-aging and anti-sunburn; the skin care product has the advantages of high safety, wide applicable range, good effects and the like, and is convenient to use, non-irritant, non-allergic and suitable for almost all people; all common skin abnormality problems can be solved with the product, besides, the skin care product takes effect rapidly, and can be used for a long time without producing toxic and side effects; the preparation method of the skin care product adopts mild conditions and a simple process, does not cause environmental pollution and is worthy of large-scale popularization.

The present invention provides a pyruvic acid determination reagent box. The reagent box comprises a reagent R1 and a reagent R2. The reagent R1 comprises the following ingredients: reduced coenzyme I(NADH), a trihydroxymethyl aminomethane (Tris) buffer solution, a surface active agent, EDTA-Na2 and a preservative. The reagent R2 comprises the following ingredients: the trihydroxymethyl aminomethane (Tris) buffer solution, lactate dehydrogenase (LDH), the surface active agent, a stabilizer and the preservative. The present also provides a preparation method of the reagent box and applications. The reagent box is a high-stability, high-accuracy and good-repeatability liquid reagent box.

The invention discloses bread yeast and an application thereof in producing a coenzyme I by fermenting. The bread yeast is prepared through the steps of after original starting strains sc-00 are mutated by implanting a low-energy nitrogen ion beam, carrying out preliminary screening on the strains, fermenting in a shake flask, and carrying out secondary screening; and the bread yeast is classified and named Saccharomycescerevisiae SC-Z180, and preserved in China Center for Type Culture Collection CCTCC, and the Preservation Number is CCTCCM2014317. The bread yeast can be used for greatly increasing the components of the coenzyme I in the process of fermentation, and reducing other components; and through carrying out fermentation and secondary screening on obtained strains, the strains can be used for significantly improving the problem that the fermentation yield is low, and in a fermentation tank with a volume of 5 L, the yield of the coenzyme I is increased by 45.8% in comparison with that of original starting strains.

The invention discloses an acne-removing, mark-eliminating and skin-protecting multifunctional preparation with both acne-removing and mark-eliminating effects and skin-beautifying and skin-protecting effects and a preparation method thereof. The acne-removing, mark-eliminating and skin-protecting multifunctional preparation is composed of a skin care composition, an acne-removing and mark-eliminating composition and a solvent, and not only contains collagen, vitamins and other various nutrients required for normal skin nutrition, but also contains a coenzyme I, a coenzyme II, nicotinamide, a coenzyme Q10 and other healthcare components required for skin health maintenance. The acne-removing, mark-eliminating and skin-protecting multifunctional preparation not only has disinfection, anticorrosion and anti-bacterium components, but also has injured tissue repair components, and the effective combination of the multiple components can generate mild acne-removing and mark-eliminating effects; the acne-removing, mark-eliminating and skin-protecting multifunctional preparation has the advantages of being high in safety, convenient to use, wide in application range, excellent in effect and the like. The acne-removing, mark-eliminating and skin-protecting multifunctional preparation disclosed by the invention is non-toxic, free from irritation and allergy and almost suitable for all people. The preparation method of the acne-removing, mark-eliminating and skin-protecting multifunctional preparation, disclosed by the invention, has the advantages of mild condition, no need of temperature rise, low energy consumption, simple and short process flow and no environmental pollution, and is a green manufacturing technology that is worth of being popularized.

The invention discloses a culture medium for rapid proliferation of neural stem cells. The culture medium is prepared from an RPMI-1640 culture medium, trehalose, recombinant human insulin, insulin growth factors, endothelial cell growth factors, potassium chloride, amino acid chelated selenium, glutamine, progesterone, coenzyme I, lipoic acid, vitamin, polygahatous polysaccharides, polysaccharide from radix codonopsis and ginseng saponin. The culture medium for rapid proliferation of the neural stem cells is good in cell wall adherence, fast in proliferation and free of animal source ingredients and does not produce residues or pollution, meanwhile the anti-pathogen microbial effect of the culture medium can be improved, and the cost of the culture medium can be reduced.