38 results about "Genetic composition" patented technology

The present invention provides methods for isolating cell free nucleic acid, e.g., apoptotic or fetal nucleic acids and methods of detecting neoplastic cells or identifying the genetic composition of a fetus. The invention also provides magnetic particles comprising an anti-DNA antibody, and kits comprising the magnetic particles.

Apparatus and methods are devised and provided to receive genetic material from a human test subject and further to characterize that genetic material into an electronic representation of a genome. In particular, with respect to many genetic features which relate to diet, nutrition and exercise with a view to weight control. Based upon the presence of certain genetic features, genetic markers or polymorphisms, a rules based logic path is executed to arrive at a action plan set of recommendations. These recommendations relate to lifestyle actions which may be taken up by a person and these actions thus relate to that person's particular genetic composition. These apparatus and methods produce an easy-to-consume visual presentation describing genetic based traits and conditions, the personal status of a test subject as it relates to these genetic dependent traits and conditions, and possible lifestyle actions which may be performed by the user.

A breeding method for the new variety of the carp relates to a breeding method of a new variety of the fish, which resolves the problems of the prior group breeding method for the culture and the cultivation of the carp that is easy to have the inbreeding, which leads to the decrease of the economic character and the production performance; the intraspecific hybridization method has great randomness, the probability for breeding an excellent variety is low and is easy to have the inbreeding. The breeding procedures are: 1. performing the phenotypic character selection; 2. detecting the genetic information of the parent strain; 3. performing the reproduction grouping according to the genetic information; 4. cultivating the filial generation to be matured; 5. adopting the offspring carp as the new generation of the breeding parent strain and repeating the operation procedures from 1 to 4 until the carp meets the character design requirements of the new variety. The present invention always uses the molecular marker to detect the genetic composition of the parent strain and the filial generation in the selection process of the new carp variety, thereby avoiding the inbreeding; moreover the character of the new variety of the carp is developed in the designed direction, the emergence probability of the excellent new variety is high; therefore, the economic character and the production performance of the carps are obviously improved.

The invention discloses a PCR primer pair used for identifying the genetic sex of Pseudobagrus ussuriensis, and a rapid identification method thereof. The identification method comprises the steps of examined individual tissue drawing, genome DNA extraction, sex specific DNA fragment PCR amplification, agarose gel electrophoresis, ultraviolet light detection shooting and examined individual genetic sex determination. High-quality genome DNA is extracted through an optimized process, a sex specific DNA fragment is amplified, and the existence or not and the existence characteristic of the sex specific fragment are detected under ultraviolet lights, so the genetic constitution of Pseudobagrus ussuriensis is identified, and the genetic sex is discriminated in 1-2d. The PCR primer pair and the rapid identification method provide a rapid identification method for identifying sex reversal fish and super-male fish, provide technical support for breeding all-male fish, and have the advantages of breeding time shortening, great increase of the output, breeding cost reduction, and profound economic and social values.

The invention discloses a method for separating ustilaginoidea virens. According to the method, a special spore dispersion filament is matched with a spore dispersion plate, single chlamydospore is directly separated from rice grains suffering from false smut, and a false smut bacterial strain with single genetic composition is formed through culture. The implementation and operation steps of the isolated culture are as follows: 1) collecting and preparing a standard sample of false smut; (2) preparing spore dispersion filaments; (3) preparing a spore dispersion plate; (4) dispersing the chlamydospores; (5) cutting out discrete single chlamydospores; and (6) culturing the monospores and forming monospore strains. The method has the following advantages: 1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; 2) single spore strains can be separated and obtained at one time; 3) the separation work consumes less time and labor; and 4) the working efficiency of Ustilaginoidea virens separation and the reliability of the separation result can be obviously improved.

The invention discloses a separation method of botrytis cinerea. According to the method, single conidia are directly separated from a botrytis cinerea standard sample by adopting a fine, soft and elastic spore dragging filament and are cultured to form a botrytis cinerea strain with single genetic composition. The implementation and operation steps of the isolated culture are as follows: (1) collecting a botrytis cinerea standard sample; (2) firing spore dragging and dispersing filaments; (3) preparing a spore dragging and dispersing plate; (4) dragging and dispersing conidia; (5) cutting small square blocks of single spores; and (6) forming a monospore isolated strain. The method has the following advantages: (1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; (2) single spore strains can be separated and obtained at one time; (3) the separation work consumes less time and labor; and (4) the working efficiency of botrytis cinerea separation and the reliability of the separation result can be obviously improved.

The present invention relates to a method and composition for the use of a neoadjuvant to be used in with existing therapies to break host immune tolerance to tumor cells or other types of diseased cells. More precisely the present invention is directed to a vaccine which delivers a polypeptide adjuvant such as HSP72, or a cytokine molecule such as GMCSF, which is encoded in a polynucleotide that will be expressed at high levels within tumor cells and may also bind to tumor cell antigens. The vaccine may be administered alone, or in conjunction with a known tumor antigen or vaccine. After a period of time sufficient for expression of the polynucleotide and for the binding of adjuvant to cancer antigens, the cancer will be treated with conventional therapies, allowing the adjuvant / antigen complex to be released to the interstitial fluids where they will be accessible to antigen presenting cells of the host immune system. The presentation of the cancer antigen complexed with adjuvant as well as cytokine stimulation of the host immune system will cause the host to mount a heightened immune response against all remaining cancer cells or tumors which share these antigens.

The invention relates to the technical field of molecular markers, in particular to a sagittaria sagittifolia SSR primer group and application thereof. According to the invention, DNA extraction, sequencing and analysis are carried out on sagittaria sagittifolia materials of different regions to obtain corresponding SSR primer groups; the primer group can be used for classifying and identifying sagittaria sagittifolia materials in several different regions, and judging the distance of genetic relationship, provides molecular biological reference basis for researching the genetic composition, genetic relationship and breeding of the sagittaria sagittifolia variety, and is a SSR primer group with high polymorphism, and can achieve good clustering and identification effects on sagittaria sagittifolia of different germplasm resources.

The present invention presents methods to alter the genetic composition of crop plants susceptible to nematode infection to improve tolerance to the same. Methods and compositions for modulating key pathways involved in the syncytial event of nematode infection and for preventing the cascade of differential gene expression caused by the same as disclosed. Applicants have found that the microRNA miR396 acts as a master switch of syncytial gene expression changes in plants after infection, and further that miR396 and certain growth regulating transcription factors (GRF) are connected through feedback interaction in syncytium initiation and maintenance.

Described herein are assays, kits and methods for treating mood disorders by testing for one or more polymorphisms in a specific group of genes and for analyzing the results of polymorphism testing; the genes included may converge in one or more signaling pathways, and may be epigenetic. The genes are included based on the relationships of the proteins encoded by the genes in the context of particular signaling pathways and provide a diagnostically relevant nexus. Also described herein are methods of presenting the data collected by the screen, including methods of delivering interpretive comments and / or treatment guidance based on the results of the genetic screening either individually or based on the genetic composition of particular clusters of genes which may be related to each other. Importantly, drugs which modulate these genetic disturbances are described for targeted therapeutic use based upon companion diagnostic method.

Apparatus and methods are devised and provided to receive genetic material from a human test subject and further to characterize that genetic material into an electronic representation of a genome. In particular, with respect to many genetic features which relate to diet, nutrition and exercise with a view to weight control. Based upon the presence of certain genetic features, genetic markers or polymorphisms, a rules based logic path is executed to arrive at a action plan set of recommendations. These recommendations relate to lifestyle actions which may be taken up by a person and these actions thus relate to that person's particular genetic composition. These apparatus and methods produce an easy-to-consume visual presentation describing genetic based traits and conditions, the personal status of a test subject as it relates to these genetic dependent traits and conditions, and possible lifestyle actions which may be performed by the user.

The invention discloses a method for drawing a genetic composition map of a wheat variety, and relates to the technical field of agricultural science and technology and molecular markers. According to the method, tens of thousands and hundreds of thousands of SNP markers are obtained by analysis of high-throughput wheat chips (such as 660K and 90K chips and the like), genetic information transmission and distribution of wheat breeding parents and offsprings having clear genealogical relationship are displayed in the form of images so as to achieve the visual display of the distribution pattern including number and distribution in chromosomes of the genetic information of the parents in genomes of offsprings, wherein according to the number, general genetic information transmission can be seen by the genetic composition map, when male and female parents are large in contribution difference, the quantity of the genetic information contributed by the male or female parents can be more clearly to see. The method facilitates better understanding of the effect of wheat breeding on the genomes from genome level, and the genomic information is provided to subsequent wheat breeding for reference.

The present invention provides methods for isolating cell free nucleic acid, e.g., apoptotic or fetal nucleic acids and methods of detecting neoplastic cells or identifying the genetic composition of a fetus. The invention also provides magnetic particles comprising an anti-DNA antibody, and kits comprising the magnetic particles.

The invention discloses a high-polymorphism single-copy microsatellite site combination and related primer combination for phoebe zhennan producing area tracing. The related primer combination for phoebe zhennan producing area tracing, disclosed by the invention, is composed of 40 single-stranded DNAs as shown in sequences 1-40 in a sequence table. By utilizing the primer combination provided by the invention, the genetic diversity of phoebe zhennan can be correctly evaluated, the origin of phoebe zhennan species can be quickly and accurately identified, and the primer combination is of great significance to protection of vulnerable species. Meanwhile, by utilizing the primer combination provided by the invention, the genetic composition of the phoebe zhennan can be conveniently determined, a phoebe zhennan fingerprint database can be conveniently established, the species source of the phoebe zhennan can be quickly and accurately identified, and a false phoebe zhennan producing area is prevented. The primer combination provided by the invention can be developed into a kit and made into a product, has a relatively wide application market, and good economic benefits are obtained.

The invention discloses a molecular breeding method of new varieties of cynoglossus semilaevis and solves the problems that in current cynoglossus semilaevis breeding and cultivation, an intraspecific hybridization method has high randomness and low good variety breeding probability and a group breeding method causes inbreeding easily and thus causes reduction of economic traits and productivity. The method comprises breeding steps as follows: 1, selecting phenotypic characters; 2, detecting and analyzing genetic composition of male and female parents; 3, selecting parents in different breeding family trees, and performing grouping with the genetic distance between the parents of 0.7-1.0 as the threshold range; 4, breeding offspring until sexual maturity; 5, with cynoglossus semilaevis offspring as a new generation of parents, repeating operation in steps 1 to 4 until meeting the design requirement of the new varieties of cynoglossus semilaevis. According to the method, during breeding of the new varieties of cynoglossus semilaevis, the breeding family tree of each generation of parents is detected all the time, grouping is performed with the genetic distance between the parents of 0.7-1.0 as the threshold range, inbreeding can be avoided, the characters of the new varieties of cynoglossus semilaevis can be selected in the set direction, the economic characters and productivity can be significantly improved, and the probability of occurrence of superior new varieties can be increased.

The invention discloses an efficient matching breeding method for procypris merus, and relates to a breeding method for procypris merus. The procypris merus protospecies are protected and continuouslyutilized, and the production performance of the procypris merus protospecies is improved. The method comprises the following steps: 1, selecting procypris merus, and performing marking and filing; 2,analyzing the genotype and genetic composition of the procypris merus parent fish population; 3, performing analysis by using software and then carrying out grouping propagation; and 4, breeding filial generations until sexual maturity to finish breeding. According to the method, the procypris merus protospecies are protected and continuously utilized, and the production performance of the procypris merus protospecies is improved.

System and methods for alerting a healthcare provider to prescribed treatments having reduced or no effectiveness due to genetic composition is provided. A database containing treatments known to have reduced or no efficacy in persons having particular genetic markers is queried to determine whether any treatments prescribed by, or likely to be prescribed by, a healthcare provider to the patient are known to have reduced or no efficacy in persons having the same genetic markers as the patient. An alert containing such information is displayed at a healthcare provider system.

The invention discloses a molecular breeding method of new varieties of cynoglossus semilaevis and solves the problems that in current cynoglossus semilaevis breeding and cultivation, an intraspecific hybridization method has high randomness and low good variety breeding probability and a group breeding method causes inbreeding easily and thus causes reduction of economic traits and productivity. The method comprises breeding steps as follows: 1, selecting phenotypic characters; 2, detecting and analyzing genetic composition of male and female parents; 3, selecting parents in different breeding family trees, and performing grouping with the genetic distance between the parents of 0.7-1.0 as the threshold range; 4, breeding offspring until sexual maturity; 5, with cynoglossus semilaevis offspring as a new generation of parents, repeating operation in steps 1 to 4 until meeting the design requirement of the new varieties of cynoglossus semilaevis. According to the method, during breeding of the new varieties of cynoglossus semilaevis, the breeding family tree of each generation of parents is detected all the time, grouping is performed with the genetic distance between the parents of 0.7-1.0 as the threshold range, inbreeding can be avoided, the characters of the new varieties of cynoglossus semilaevis can be selected in the set direction, the economic characters and productivity can be significantly improved, and the probability of occurrence of superior new varieties can be increased.

System and methods for alerting a healthcare provider to prescribed treatments having reduced or no effectiveness due to genetic composition is provided. A database containing treatments known to have reduced or no efficacy in persons having particular genetic markers is queried to determine whether any treatments prescribed by, or likely to be prescribed by, a healthcare provider to the patient are known to have reduced or no efficacy in persons having the same genetic markers as the patient. An alert containing such information is displayed at a healthcare provider system.

The invention discloses a method for isolating banana anthracnose bacteria. In this method, a special spore discrete silk is used to directly separate a single conidia from a standard sample of banana anthracnose, and culture it to form a strain of banana anthracnose with a single genetic composition. The implementation steps of isolation culture are as follows: 1) preparation of banana anthracnose standard sample; 2) preparation of spore discrete silk; 3) preparation of spore discrete plate; 4) separation of conidia; 5) small squares loaded with single spores 6) the formation of single spore isolated strains. The present invention has the following advantages: 1) the key utensils for separating spores are simple and easy to make, and the separation operation is simple and easy; 2) the separation and acquisition of monospore strains can be done at one time; 3) the separation work consumes less labor and time; 4) can Significantly improve the work efficiency and the reliability of the isolation results of banana anthracnose bacteria isolation.

The invention provides a new insect genetical population simulating method based on natural reproduction process. In the method, a primary sample sequence marked by an insect parent molecule is utilized as the genetic composition of a parent, number vectors consisting of 1 and 0 are used for representing the genetic markers of chromosomes and gametes, and the genetic distance between the genetic markers is converted into recombination frequency by a mapping function. When F1 half-bred by two parents generates a gamete, the exchange process of reduction division is simulated by simple samplings which are uniformly and randomly distributed; and individual inspect is simulated by repeated gamete sampling and zygote forming, so that a genetic group is formed. The method can be used for the exploration and the evaluation on an insect gene mapping method, and the dynamic change of the insect resistance genes in generations can be researched through the genetic markers, without years of multipoint practical field tests, so that the efficiency of genetic research is improved greatly. The new insect genetical population simulating method is applied to crop production and pest prevention management and has very good positive results.

The invention discloses a method for isolating rice blast fungus. The method adopts a special spore slipping filament, directly separates a single conidia from a rice blast standard sample, and cultures it to form a single genetic composition of the rice blast fungus strain. The implementation steps of isolation and cultivation are as follows: 1) collection of rice blast standard sample; 2) disposal of rice blast fungus standard sample; 3) preparation of spore slip loose silk; 4) preparation of spore slip loose plate; 5) conidia 6) cutting the spore-carrying sliding plate; 7) the cultivation of conidia and the formation of monospore strains. The present invention has the following advantages: 1) the key utensils for separating spores are simple and easy to make, and the separation operation is simple and easy; 2) the separation and acquisition of monospore strains can be done at one time; 3) the separation work consumes less labor and time; 4) can Significantly improve the efficiency of the isolation of blast fungus and the reliability of the isolation results.