31 results about "Genetic composition" patented technology

The present invention provides methods for isolating cell free nucleic acid, e.g., apoptotic or fetal nucleic acids and methods of detecting neoplastic cells or identifying the genetic composition of a fetus. The invention also provides magnetic particles comprising an anti-DNA antibody, and kits comprising the magnetic particles.

Apparatus and methods are devised and provided to receive genetic material from a human test subject and further to characterize that genetic material into an electronic representation of a genome. In particular, with respect to many genetic features which relate to diet, nutrition and exercise with a view to weight control. Based upon the presence of certain genetic features, genetic markers or polymorphisms, a rules based logic path is executed to arrive at a action plan set of recommendations. These recommendations relate to lifestyle actions which may be taken up by a person and these actions thus relate to that person's particular genetic composition. These apparatus and methods produce an easy-to-consume visual presentation describing genetic based traits and conditions, the personal status of a test subject as it relates to these genetic dependent traits and conditions, and possible lifestyle actions which may be performed by the user.

The invention discloses a PCR primer pair used for identifying the genetic sex of Pseudobagrus ussuriensis, and a rapid identification method thereof. The identification method comprises the steps of examined individual tissue drawing, genome DNA extraction, sex specific DNA fragment PCR amplification, agarose gel electrophoresis, ultraviolet light detection shooting and examined individual genetic sex determination. High-quality genome DNA is extracted through an optimized process, a sex specific DNA fragment is amplified, and the existence or not and the existence characteristic of the sex specific fragment are detected under ultraviolet lights, so the genetic constitution of Pseudobagrus ussuriensis is identified, and the genetic sex is discriminated in 1-2d. The PCR primer pair and the rapid identification method provide a rapid identification method for identifying sex reversal fish and super-male fish, provide technical support for breeding all-male fish, and have the advantages of breeding time shortening, great increase of the output, breeding cost reduction, and profound economic and social values.

The invention discloses a method for separating ustilaginoidea virens. According to the method, a special spore dispersion filament is matched with a spore dispersion plate, single chlamydospore is directly separated from rice grains suffering from false smut, and a false smut bacterial strain with single genetic composition is formed through culture. The implementation and operation steps of the isolated culture are as follows: 1) collecting and preparing a standard sample of false smut; (2) preparing spore dispersion filaments; (3) preparing a spore dispersion plate; (4) dispersing the chlamydospores; (5) cutting out discrete single chlamydospores; and (6) culturing the monospores and forming monospore strains. The method has the following advantages: 1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; 2) single spore strains can be separated and obtained at one time; 3) the separation work consumes less time and labor; and 4) the working efficiency of Ustilaginoidea virens separation and the reliability of the separation result can be obviously improved.

The invention discloses a separation method of botrytis cinerea. According to the method, single conidia are directly separated from a botrytis cinerea standard sample by adopting a fine, soft and elastic spore dragging filament and are cultured to form a botrytis cinerea strain with single genetic composition. The implementation and operation steps of the isolated culture are as follows: (1) collecting a botrytis cinerea standard sample; (2) firing spore dragging and dispersing filaments; (3) preparing a spore dragging and dispersing plate; (4) dragging and dispersing conidia; (5) cutting small square blocks of single spores; and (6) forming a monospore isolated strain. The method has the following advantages: (1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; (2) single spore strains can be separated and obtained at one time; (3) the separation work consumes less time and labor; and (4) the working efficiency of botrytis cinerea separation and the reliability of the separation result can be obviously improved.

The present invention relates to a method and composition for the use of a neoadjuvant to be used in with existing therapies to break host immune tolerance to tumor cells or other types of diseased cells. More precisely the present invention is directed to a vaccine which delivers a polypeptide adjuvant such as HSP72, or a cytokine molecule such as GMCSF, which is encoded in a polynucleotide that will be expressed at high levels within tumor cells and may also bind to tumor cell antigens. The vaccine may be administered alone, or in conjunction with a known tumor antigen or vaccine. After a period of time sufficient for expression of the polynucleotide and for the binding of adjuvant to cancer antigens, the cancer will be treated with conventional therapies, allowing the adjuvant/antigen complex to be released to the interstitial fluids where they will be accessible to antigen presenting cells of the host immune system. The presentation of the cancer antigen complexed with adjuvant as well as cytokine stimulation of the host immune system will cause the host to mount a heightened immune response against all remaining cancer cells or tumors which share these antigens.

The present invention presents methods to alter the genetic composition of crop plants susceptible to nematode infection to improve tolerance to the same. Methods and compositions for modulating key pathways involved in the syncytial event of nematode infection and for preventing the cascade of differential gene expression caused by the same as disclosed. Applicants have found that the microRNA miR396 acts as a master switch of syncytial gene expression changes in plants after infection, and further that miR396 and certain growth regulating transcription factors (GRF) are connected through feedback interaction in syncytium initiation and maintenance.

The invention discloses a high-polymorphism single-copy microsatellite site combination and related primer combination for phoebe zhennan producing area tracing. The related primer combination for phoebe zhennan producing area tracing, disclosed by the invention, is composed of 40 single-stranded DNAs as shown in sequences 1-40 in a sequence table. By utilizing the primer combination provided by the invention, the genetic diversity of phoebe zhennan can be correctly evaluated, the origin of phoebe zhennan species can be quickly and accurately identified, and the primer combination is of great significance to protection of vulnerable species. Meanwhile, by utilizing the primer combination provided by the invention, the genetic composition of the phoebe zhennan can be conveniently determined, a phoebe zhennan fingerprint database can be conveniently established, the species source of the phoebe zhennan can be quickly and accurately identified, and a false phoebe zhennan producing area is prevented. The primer combination provided by the invention can be developed into a kit and made into a product, has a relatively wide application market, and good economic benefits are obtained.

The invention discloses a molecular breeding method of new varieties of cynoglossus semilaevis and solves the problems that in current cynoglossus semilaevis breeding and cultivation, an intraspecific hybridization method has high randomness and low good variety breeding probability and a group breeding method causes inbreeding easily and thus causes reduction of economic traits and productivity. The method comprises breeding steps as follows: 1, selecting phenotypic characters; 2, detecting and analyzing genetic composition of male and female parents; 3, selecting parents in different breeding family trees, and performing grouping with the genetic distance between the parents of 0.7-1.0 as the threshold range; 4, breeding offspring until sexual maturity; 5, with cynoglossus semilaevis offspring as a new generation of parents, repeating operation in steps 1 to 4 until meeting the design requirement of the new varieties of cynoglossus semilaevis. According to the method, during breeding of the new varieties of cynoglossus semilaevis, the breeding family tree of each generation of parents is detected all the time, grouping is performed with the genetic distance between the parents of 0.7-1.0 as the threshold range, inbreeding can be avoided, the characters of the new varieties of cynoglossus semilaevis can be selected in the set direction, the economic characters and productivity can be significantly improved, and the probability of occurrence of superior new varieties can be increased.

The invention discloses an efficient matching breeding method for procypris merus, and relates to a breeding method for procypris merus. The procypris merus protospecies are protected and continuouslyutilized, and the production performance of the procypris merus protospecies is improved. The method comprises the following steps: 1, selecting procypris merus, and performing marking and filing; 2,analyzing the genotype and genetic composition of the procypris merus parent fish population; 3, performing analysis by using software and then carrying out grouping propagation; and 4, breeding filial generations until sexual maturity to finish breeding. According to the method, the procypris merus protospecies are protected and continuously utilized, and the production performance of the procypris merus protospecies is improved.

System and methods for alerting a healthcare provider to prescribed treatments having reduced or no effectiveness due to genetic composition is provided. A database containing treatments known to have reduced or no efficacy in persons having particular genetic markers is queried to determine whether any treatments prescribed by, or likely to be prescribed by, a healthcare provider to the patient are known to have reduced or no efficacy in persons having the same genetic markers as the patient. An alert containing such information is displayed at a healthcare provider system.

The invention provides a new insect genetical population simulating method based on natural reproduction process. In the method, a primary sample sequence marked by an insect parent molecule is utilized as the genetic composition of a parent, number vectors consisting of 1 and 0 are used for representing the genetic markers of chromosomes and gametes, and the genetic distance between the genetic markers is converted into recombination frequency by a mapping function. When F1 half-bred by two parents generates a gamete, the exchange process of reduction division is simulated by simple samplings which are uniformly and randomly distributed; and individual inspect is simulated by repeated gamete sampling and zygote forming, so that a genetic group is formed. The method can be used for the exploration and the evaluation on an insect gene mapping method, and the dynamic change of the insect resistance genes in generations can be researched through the genetic markers, without years of multipoint practical field tests, so that the efficiency of genetic research is improved greatly. The new insect genetical population simulating method is applied to crop production and pest prevention management and has very good positive results.

The invention relates to the technical field of crop hybrid breeding, and discloses a technology for improving a high-quality thermo-sensitive genic male sterile rice material by a grouping shuttle method, which comprises the following steps of: 1, collecting resources, identifying genetic composition of the resources by using a conventional and molecular technology, and dividing the resources into a female parent class A and a male parent class B according to the requirements of hybrid seeds on male and female parents; and step 2, classifying the collected resources. According to the technology for improving the high-quality thermo-sensitive genic male sterility rice material through the grouping shuttle method, classification is carried out according to germplasm resource sources, identification is carried out through a molecular technology, main resource materials needing to be improved and utilized can be divided into a class A and a class B according to the genetic relationship, enough target character genes exist in the classes as much as possible, and therefore the purpose of improving the high-quality thermo-sensitive genic male sterility rice material is achieved. The genetic relationship among the categories is as far as possible, the gene difference is as large as possible, and favorable genes can be fully expressed by placing the categories in different environments in turn during selection, especially under an adversity condition.