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A kind of isolation method of rice blast fungus

A rice blast fungus and separation method technology, applied in the field of plant pathology, can solve problems such as difficult operation, and achieve the effects of improving efficiency, short time consumption, and simple and easy separation operation.

Active Publication Date: 2022-06-17
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct picking without auxiliary technology devices is difficult to operate, and generally requires the operator to have good micromanipulation skills

Method used

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  • A kind of isolation method of rice blast fungus
  • A kind of isolation method of rice blast fungus
  • A kind of isolation method of rice blast fungus

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] 3. Preparation of Spore Sliding Loose Filaments

[0042] Take the ordinary disposable water pen refill that has no ink after use and burn it on the flame of the alcohol lamp, and the refill will melt where the fire is burning. Use the alcohol lamp fire extinguisher and other appliances to safely extinguish the fire on the refill, and clamp a little with pointed tweezers. The melted refill is gently stretched until a hair-like filament is formed. After cooling, the excess filament is cut off, and the filament about 3cm long is retained and connected to the pen holder to form a separate rice blast fungus. The spore sliding wire used by spores, the sliding wire tool includes the sliding wire a and the screw part b, such as figure 1 As shown, the two parts are naturally connected into one, and the key technical role is to slide silk a. The sliding wire a is small, soft and elastic.

[0043] When separating different specimens for use, first wipe the sliding wire a with a ...

Embodiment 1

[0064] The rice blast fungus of the rice blast standard sample 1 was isolated by the method for separating the rice blast fungus of the present invention, and the isolation and culture results were as follows: Image 6 shown.

Embodiment 2

[0066] The rice blast fungus of the rice blast standard sample 2 was isolated by the method for separating the rice blast fungus of the present invention, and the isolation and culture results were as follows: Figure 7 shown.

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Abstract

The invention discloses a method for isolating rice blast fungus. The method adopts a special spore slipping filament, directly separates a single conidia from a rice blast standard sample, and cultures it to form a single genetic composition of the rice blast fungus strain. The implementation steps of isolation and cultivation are as follows: 1) collection of rice blast standard sample; 2) disposal of rice blast fungus standard sample; 3) preparation of spore slip loose silk; 4) preparation of spore slip loose plate; 5) conidia 6) cutting the spore-carrying sliding plate; 7) the cultivation of conidia and the formation of monospore strains. The present invention has the following advantages: 1) the key utensils for separating spores are simple and easy to make, and the separation operation is simple and easy; 2) the separation and acquisition of monospore strains can be done at one time; 3) the separation work consumes less labor and time; 4) can Significantly improve the efficiency of the isolation of blast fungus and the reliability of the isolation results.

Description

technical field [0001] The present invention relates to plant pathology techniques. Specifically, it is an isolation method of rice blast fungus. Background technique [0002] Rice blast is a major disease of rice production in my country, which is caused by the infection of Magnaportheoryzae. Carrying out biological research on rice blast fungus is an important basis for rice blast prevention and control. The premise of carrying out biological research on rice blast fungus is to have rice blast fungus material. Generally, laboratories often need to isolate and obtain rice blast fungus from disease standard samples. [0003] The genetic composition of M. oryzae populations is complex, and the biological characteristics and pathogenicity of different strains are often quite different. Normal research usually needs to obtain the material of rice blast fungus with a single genetic composition, or monoclonal strain material. It is generally considered in the art that a stra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00
CPCC12N3/00C12N1/14C12N1/02C12M33/02
Inventor 张容博张君成王忠文
Owner GUANGXI UNIV
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