The invention discloses an artificial preparation method of white muscardine silkworms. The method includes the steps of 1, preparation of beauveria bassiana spore liquid, wherein white muscardine silkworm bodies are soaked and cleaned with sterile water, filtrate is obtained after filtering is completed, and the beauveria bassiana spore liquid is obtained after primary slant culture, secondary slant culture and tertiary slant culture; 2, pathogen toxin counteracting, wherein the beauveria bassiana spore liquid is rapidly sprayed to the silkworm bodies of 5-day-old or older silkworms within 45 min after the beauveria bassiana spore liquid is prepared, and a silkworm room is sealed for 1-1.5 h after the beauveria bassiana spore liquid is sprayed; 3, silkworm body culture, wherein the silkworm room is opened, the silkworm bodies are fed, and the silkworm bodies are fed once every other 3-4 h till the silkworm bodies die; 4, white muscardine silkworm culture, wherein the dead silkworm bodies are transferred to a silkworm rearing bed, the silkworm bodies continue to be cultured under the condition that the temperature is 25-28 DEG C and the humidity is 95-98% till the silkworm bodies are fossilized, and white mycelium conidia grow out; 5, preparation of the finished white muscardine silkworms, wherein white muscardine silkworms with the white mycelium conidia growing out are collected and then dried till the water content is smaller than 10%, and the finished white muscardine silkworms are obtained. The preparation period of the finished white muscardine silkworms is short, and the yield and quality are high.