Trichoderma asperellum with growth-promoting effect and culture method and application thereof

A technology of Trichoderma aculeatus and culture medium, applied in the field of microorganisms, can solve the problems of reducing the total amount and no effect of crop growth promotion, etc., and achieve the effects of increasing ventilation, shortening fermentation cycle, and improving production

Active Publication Date: 2017-09-08
KINGENTA ECOLOGICAL ENG GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chlamydospores of Trichoderma aculeatus in this invention obviously promote the volatilization of arsenic in the soil, reduce the total a

Method used

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  • Trichoderma asperellum with growth-promoting effect and culture method and application thereof
  • Trichoderma asperellum with growth-promoting effect and culture method and application thereof
  • Trichoderma asperellum with growth-promoting effect and culture method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0075] A strain of Trichoderma asperellum wood-8, preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on October 21, 2015, address: Chinese Academy of Sciences, No. 1, Yard 1, Beichen West Road, Chaoyang District, Beijing Institute of Microbiology, deposit number: CGMCC NO.11528.

[0076] Preparation of solid fermentation medium

[0077] The preparation method of Trichoderma aculeatus solid fermentation medium comprises the following steps:

[0078] A. Take rice husk, corn stalks, bran, and corn flour according to the above mass ratio, and mix them uniformly to obtain solid materials;

[0079] B. Weigh sucrose, (NH 4 ) 2 SO 4 、KH 2 PO 4 0.5%, MgSO 4 0.25%, FeSO 4 0.003%, mixed together to obtain a mixture of inorganic salts;

[0080] C. Dissolve the inorganic salt mixture in step B in water, mix evenly with the solid material in step A in a mass ratio of 1:1.2, and the pH is natural; the mixed solid medium is steril...

Embodiment 2

[0087] With the Trichoderma asperellum (Trichoderma asperellum) wood-8 bacterial strain culture method described in embodiment 1, difference is:

[0088] (1) Strain activation: the Trichoderma aculeatus strain preserved at 4°C was inoculated on a PDA medium plate, cultivated at 28°C for 4 days, and set aside;

[0089] (2) Prepare the shake flask seed liquid: the Trichoderma aculeatus bacterial classification that will activate is good, extract a ring and be inoculated in the triangular flask that 200ml liquid seed culture medium is housed with the inoculation loop, the triangular flask is placed in the constant temperature oscillation incubator, 28°C, 150rpm, shaking culture for 36h, the obtained shake flask seed solution;

[0090] (3) Preparation of first-class seeds: inoculate the shake flask seed liquid at a ratio of 0.5% to the first-class seed medium, 28° C., 150 rpm, and stir for 36 hours to obtain the first-class seed liquid;

[0091] (4) Solid fermentation: insert the...

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Abstract

The invention relates to a solid fermentation production method of trichoderma asperellum. The trichoderma asperellum bacterial strain used in the method is preserved in the general microbiological center of Chinese general microorganism strain preservation management committee, the preservation date is October 27th, 2015, and the preservation number is CGMCC No.11528. The trichoderma asperellum is subjected to strain activation, shake flask seed preparation, primary liquid seed culture and then solid fermentation. A solid fermentation culture medium for solid fermentation is composed of rice husks, corn straw powder, wheat bran or corn powder and inorganic salt. According to the solid fermentation method of the trichoderma asperellum, a large quantity of conidia can be generated, the spore number of the substrate trichoderma asperellum can reach up to 1*109-1*1010 cfu/g, the fermentation viable count is high, neither pollution nor waste materials are generated in the production process, and the efficiency of the production technology is high. Fermentation products obtained after fermentation have obvious growth-promoting effects on crops and greatly improve the economic benefit of the crops.

Description

technical field [0001] The invention relates to a growth-promoting Trichoderma aculeatus and its cultivation method and application, belonging to the technical field of microbes. Background technique [0002] Trichoderma spp. is a filamentous fungus with wide adaptability, against various soil-borne plant pathogenic microorganisms such as Rhizoctonia solani, Pythium spp., and Fusarium spp. It has a good control effect and has a good application prospect in the field of biological control and environmental protection. It is one of the most widely used biocontrol bacteria at present. Trichoderma can control the infection of pathogenic bacteria and the occurrence of diseases through various mechanisms such as antagonism, reparasitism, and secretion of antibiotics. There are many reports at home and abroad on the use of Trichoderma to control plant diseases, especially soil-borne diseases such as root rot, fusarium wilt, sclerotinia and sheath blight, and good control effects h...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 徐文凤顾志光胡红涛范玲超胡兆平
Owner KINGENTA ECOLOGICAL ENG GRP
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