74 results about "Viable count" patented technology

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention belongs to the technical field of biology, and particularly relates to a complex microbial inoculant for promoting formation of a biological membrane, a microcapsule and application of the complex microbial inoculant. The complex microbial inoculant comprises lactobacillus mucus fermentation FMBL L24016DMX with the preservation number of CCTCC NO: M20241583 and bifidobacterium longum subsp. Longum FMBL B241770LS with the preservation number of CCTCC NO: M 20241993, the complex microbial inoculant can grow on a culture medium containing prebiotics, the biofilm formation amount is the highest when the complex microbial inoculant is co-cultured in a culture medium containing isomaltooligosacharide, the complex microbial inoculant is prepared into a single-layer microcapsule or a double-layer microcapsule, and the complex microbial inoculant can be used for preparing the probiotics. The endophytic fungus has good embedding rate and viable count, can resist gastrointestinal environment, has good heat resistance, and has wide application prospects.

The invention discloses a method for improving antioxidant activity of oil cakes through multi-strain fermentation combined with enzymolysis and application. The method comprises the following steps: adding a multi-strain mixed bacterium suspension into sterilized oil cakes, uniformly mixing, performing constant-temperature fermentation at 30 + / -2 DEG C, performing intermittent stirring in the fermentation process, and sequentially performing high-temperature sterilization and freeze-drying treatment after the fermentation is finished, the multi-strain mixed bacterium suspension is a mixed bacterium suspension of aspergillus niger, saccharomyces cerevisiae and bacillus subtilis, and the viable count ratio of the aspergillus niger to the saccharomyces cerevisiae to the bacillus subtilis in the multi-strain mixed bacterium suspension is (1-2): (1-2): (1-2). The application finds that a multi-strain composite fermentation combined enzymolysis technology can realize the synergistic interaction of fermentation and enzymolysis, obviously improves the antioxidant activity of the oil cake meal, and can be applied to preparation of cake meal feed.

The invention belongs to the field of fermentation engineering, and particularly discloses a preparation method and application of edible fungus and probiotic co-culture fermentation liquor. A bidirectionally promoted edible fungus-probiotic symbiotic metabolism system is constructed by adopting a synchronous inoculation technology and combining with an optimized culture medium formula taking grain processing materials such as coarse rice powder as a main substrate. According to the method, in the culture period of 7-14 days, the fermentation liquor with the total triterpene content not lower than 35 mg / g, the total polysaccharide content not lower than 25 mg / g and the viable count of probiotics reaching 1 * 10 < 8 > CFU / mL or above can be obtained. The technology has the advantages of being easy and convenient to operate, low in cost and short in period, the content of the active ingredients of the edible fungi is remarkably increased while the viable count of the probiotics is maintained, and a new technical scheme is provided for developing high-quality functional food or preparations with the probiotic function and the active ingredients of the fungi.

The invention relates to the technical field of microbial preparations for agricultural environment restoration, and provides a microbial agent for reducing pesticide residues in soil, which is characterized by comprising the following components in parts by weight: 30-60 parts of ochrobactrum having viable count of more than or equal to 5 * 10 < 8 > cfu / g; the spore number is greater than or equal to 2 * 10 < 8 > cfu / g. The final residues of imidacloprid and difenoconazole in the compounded microbial agent are reduced to about 0.20 mg / kg, balanced and efficient removal is realized, and the possible unbalance situation of'degrading one pesticide and remaining one pesticide 'in the traditional method is avoided.

The application provides a preparation method of an antioxidant Aeschynomene indica L. probiotic fermentation liquor, and belongs to the technical field of biological fermentation and microbial agents. The method comprises the following steps: step one, respectively preparing an Aeschynomene indica L. extract and a probiotic seed liquor; and step two, inoculating the seed liquor into the Aeschynomene indica L. extract or a culture medium containing the Aeschynomene indica L. extract for fermentation to obtain the Aeschynomene indica L. probiotic fermentation liquor. The Aeschynomene indica L. probiotic fermentation liquor provided by the application can effectively maintain the viable count of probiotics so that the probiotics can exert the probiotic effect, and the Aeschynomene indica L. water extract itself also has multiple probiotic activities, and the content of antioxidant metabolites is obviously increased after fermentation.

The application belongs to the technical field of microorganism detection, and particularly relates to a bifidobacterium viable count detection culture medium, a detection method and application. The application provides a culture medium for detecting bifidobacterium, wherein the culture medium comprises MRS culture medium and an antibiotic solution; the antibiotic solution comprises streptomycin, kanamycin and sodium propionate. The culture medium can ensure normal growth of bifidobacterium, has strong inhibition on lactobacillus, can effectively realize accurate detection of bifidobacterium, and avoids interference of lactobacillus.

The present application provides a kind of wettable powder and its preparation method and application, belong to forest disease biological control technical field.The wettable powder of the present application is made of the following mass fraction components: Bacillus subtilis WB1 fermentation liquor 90-110 parts, filler 42-48 parts, auxiliary 28-32 parts.The wettable powder of Bacillus subtilis WB1 prepared by the present application has high viable count, good suspensibility, dispersibility and wettability, can be stored, transported and sprayed in large area in forest at room temperature for a long time, has broad-spectrum antibacterial property, and has good control effect on walnut bacterial black spot.The preparation method of the wettable powder of the present application is simple, low in cost, and suitable for large-scale popularization and application.

The invention provides an efficient microbial fermentation inoculant for straws and a preparation method thereof, and relates to the technical field of biodegradation. The efficient microbial fermentation inoculant for the straw is formed by compounding four strains including trichoderma reesei, streptomycete, lactobacillus plantarum and bacillus amyloliquefaciens according to the mass ratio, the ratio of the viable count of the four strains is that the ratio of the viable count of the trichoderma reesei to the viable count of the streptomycete to the viable count of the lactobacillus plantarum to the viable count of the bacillus amyloliquefaciens is 3: 2: 2: 1.5, the total viable count of the inoculant is larger than or equal to 1.3 * 10 CFU / g, and the cellulase activity is larger than or equal to 85 U / g. The laccase activity is greater than or equal to 42U / g. The invention can effectively solve the industrial pain points of difficult degradation of straws, poor quality and weak stability, and in agricultural production, the microbial inoculum can increase the content of organic matters in soil, can reduce the feed cost in the feed field, and can be used for production of straw-based organic fertilizers in the environmental protection field, and can reduce the use amount of chemical fertilizers.

This invention discloses a composite microbial agent for improving saline-alkali soil, its preparation method, and its application. The composite microbial agent is composed of *Frederick's chamaejasminoides* and *Zoococcus halophilus* with a viable count ratio of (0.2–2):1. Research shows that, under the same total viable count conditions, the composite microbial agent exhibits significantly higher salt tolerance, IAA production, siderophore production capacity, ACC deaminase activity, phosphorus solubility, nitrogen fixation capacity, and extracellular polysaccharide production compared to single-strain agents, demonstrating a significant synergistic effect. The composite agent can also be combined with the salt-tolerant plant *Arundo donax* to construct a synergistic remediation system for saline-alkali land. This system significantly reduces the pH and sodium ion content of saline-alkali soil, improves soil nutrient availability, and significantly promotes *Arundo donax* growth and biomass accumulation, providing an innovative technical solution for rapid, efficient, and sustainable ecological restoration and agricultural utilization of saline-alkali land.

This invention relates to the field of functional microbial screening and application technology, specifically providing a probiotic crystal ball and its preparation method. The probiotic crystal ball consists of three parts: a crystal nucleus, a crystal mantle, and a crystal shell. The crystal nucleus contains *Lactobacillus plantarum* powder. The *Lactobacillus plantarum* strain is VHProbi R14, with accession number CCTCC NO: M2023721. This strain can effectively regulate the intestinal flora and significantly increase the abundance of beneficial *Akermansia* species, especially *Akermansia myxophilus* (Akk bacteria). The probiotic crystal ball provided by this invention has a two-layer protective structure, exhibiting excellent stability and heat resistance. It can effectively maintain the viable count of *Lactobacillus plantarum* VHProbi R14 during transportation, storage, and processing, significantly improving the applicability of *Lactobacillus plantarum* VHProbi R14, and can be widely used in food, health products, and other fields.

This invention discloses a *Lactobacillus plantarum* bacterial powder, its preparation method, and its application. The wall material of the bacterial powder includes resistant starch, whey protein, and a heat protectant. The bacterial cells in the powder are *Lactobacillus plantarum* TH103. The mass percentages of resistant starch, whey protein, and the heat protectant are 7-23%, with the total mass percentage of resistant starch and whey protein being 3-15% and the mass percentage of the heat protectant being 4-8%. The viable count of the bacterial powder is (3.5-9.0) × 10⁻⁶. 9 The CFU / g and moisture content are 5-7%. This invention uses resistant starch and whey protein as protective carriers, supplemented with heat-resistant protective agents, to achieve the preparation of *Lactobacillus plantarum* powder by spray drying. The obtained *Lactobacillus plantarum* powder has a viable count and moisture content that meet the relevant requirements for probiotic foods, and has good powder properties. It can be directly brewed in powder form or applied to fermented foods such as yogurt.

The invention belongs to the technical field of quantitative detection of microorganisms, and particularly relates to a method for quantitatively detecting the viable count of mycoplasma bovis. The method for quantitatively detecting the viable count of the mycoplasma bovis is finally obtained by exploring the linear relation between the protein concentration and the viable count of multiple batches of mycoplasma bovis liquid samples after optimization of experimental conditions such as bacterium liquid preservation conditions, ultrasonic crushing modes, crushing duration and sample treatment modes, and the method is good in stability, high in timeliness and high in accuracy. The detection result is accurate, and a convenient and effective method is provided for rapid detection of the viable count of the mycoplasma bovis liquid.

The invention discloses a pseudomonas aeruginosa (Pseudomonas aeruginosa) T-5 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20251946, a microbial agent of the pseudomonas aeruginosa (Pseudomonas aeruginosa) T-5, and an application of the pseudomonas aeruginosa (Pseudomonas aeruginosa) T-5. The strain is gram-negative bacteria, cells are rod-shaped, bacterial colonies are large, the center of the strain protrudes and is milky white, and blue-green or tawny pigments can be generated; when the strain is cultured in an LB liquid culture medium under the conditions of 30 DEG C and 160 rpm, the strain enters a logarithmic phase in 2-4 h and enters a stable phase in 24 h, the OD600 value in the stable phase reaches 1.6 + / -0.1, and the viable count of fermentation liquor in 24 h is larger than or equal to 10 < 10 > CFU / mL; and the method is suitable for repairing a severe environment with composite organic pollution.

The invention discloses a lactobacillus plantarum freeze-drying protective agent, freeze-drying bacterial powder as well as a preparation method and application of the freeze-drying bacterial powder, and belongs to the technical field of microorganisms. The lactobacillus plantarum freeze-drying protective agent comprises the following raw materials: saccharides, milk powder and sodium salt. The mass ratio of the saccharides to the milk powder to the sodium salt is (4-6): (14-16): (4-6). The lactobacillus plantarum freeze-drying protective agent can relieve damage caused by ice crystals in the freeze-drying process of lactobacillus plantarum and maintain and stabilize protein and cell structures so as to effectively protect thalli, and the viable count and the cell survival rate of freeze-dried lactobacillus plantarum are improved.

The invention belongs to the field of microorganisms, and particularly relates to lactobacillus paracasei with multi-matrix fermentation capacity and application of the lactobacillus paracasei. The lactobacillus paracasei is lactobacillus paracasei Shannan.LPA-972, and the preservation number of the lactobacillus paracasei Shannan.LPA-972 is CCTCC (China Center For Type Culture Collection) NO: M 20242026. The lactobacillus paracasei is lactobacillus paracasei Shannan.LPA-972. The lactobacillus paracasei is related to a phosphoenolpyruvate-glycotransferase system carried by the lactobacillus paracasei. When the fermentation agent containing lactobacillus paracasei, lactobacillus delbrueckii subsp. Lactis and streptococcus thermophilus subsp. Salivarius with a specific viable count ratio is used for fermenting plant milk and animal milk, broad-spectrum adaptability of animal milk raw materials and plant milk raw materials is achieved, meanwhile, the nutrition and sensory quality of fermentation products are remarkably improved, the problem of multi-matrix fermentation can be effectively solved, and the production cost is reduced. And the process cost and complexity of a dairy product production end are effectively reduced.

The invention relates to a probiotic-bacterial cellulose composite membrane as well as a preparation method and application thereof. The probiotic-bacterial cellulose composite membrane is prepared from lactobacillus plantarum LCC-605 with the preservation number of CCTCC No: M 2016491, a biological membrane, a metabolite and a postbiotic of the metabolite, and the probiotic-bacterial cellulose composite membrane is prepared from lactobacillus plantarum LCC-605 with the preservation number of CCTCC No: M 2016491. The method comprises the following steps: carrying out in-situ co-culture on Acetobacter xylinum and Lactobacillus plantarum LCC-605 to form a Lactobacillus plantarum biofilm based bacterial cellulose membrane and related metabolites of the Lactobacillus plantarum biofilm based bacterial cellulose membrane, and carrying out in-situ co-culture on Acetobacter xylinum and Lactobacillus plantarum LCC-605 to form the Lactobacillus plantarum biofilm based bacterial cellulose membrane. The invention not only provides the bacterial cellulose membrane for encapsulating the probiotics to enhance the survival rate and the stability of the probiotics, but also provides an ideal leavening agent for preparing the fermented black bean milk with excellent texture, high viable count and excellent storage stability.

This invention provides an artificial humic acid-graphene composite microbial agent, its preparation method, and its application. The microbial agent includes an artificial humic acid-graphene composite carrier and functional microorganisms loaded on its surface and in the gaps of its aggregated structure. The artificial humic acid modification layer is bonded to the surface of the graphene material through non-covalent and covalent bonds. This composite carrier has excellent water retention and ion buffering capabilities, creating a localized protective microenvironment for microorganisms. The microbial agent prepared using this carrier can stably maintain an effective viable count ≥1.0 × 10⁻⁶. 9 With a CFU / g concentration, and a survival rate under drought and salt stress that is more than 50% higher than that of traditional carrier inoculants, it fundamentally solves the industry problem that live bacteria do not survive long when applied to the ground.

This invention discloses a method for controlling root rot of Panax notoginseng based on a fungal synthetic microbial community and its application, belonging to the field of plant disease biological control technology. The method involves screening six fungal strains from the rhizosphere soil of healthy Panax notoginseng plants that have survived for three years in continuously cropped fields. These strains exhibit highly efficient antagonistic activity (inhibition rate ≥70%) against three pathogens of the genus *Fusarium*. A synthetic microbial community containing *Trichoderma atroviride*, *Trichoderma hamatum*, *Trichoderma petersenii*, *Trichoderma neokoningii*, *Trichoderma virilente*, and *Phanerochaete concrescens* was constructed at a viable count ratio of 1:350:590:14:30:755, and prepared to a spore concentration of 10... 8 -10 9 Capsules containing 10⁸ CFU / g are used. Before planting, apply the fertilizer to the bottom of the planting hole. After emergence, apply the fertilizer using a fertilizer applicator within a 5cm radius around the roots, 20-30g per plant (based on 10⁸ CFU / g). Apply once a month from 4-6 months after emergence, for three consecutive applications. During the application period, maintain soil moisture at 60%-70% and a temperature of 13-20℃. This method can reduce the incidence of root rot in Panax notoginseng to below 10.60%, while increasing yield per plant by 6% and increasing total saponin content by ≥20%, combining prevention, growth promotion, and environmental safety.

The present application relates to a kind of microbial composition and its application in the ecological prevention and control of banana wilt, belong to the field of biotechnology, the microbial composition includes the volume of 2-3:1-4:1 Amycolata bacterial liquid, Moore Morganella bacterial liquid and lysobacter bacterial liquid;Effective viable count in the Amycolata bacterial liquid and Moore Morganella bacterial liquid is all not less than 1×10 8 CFU / mL, effective viable count in lysobacter bacterial liquid is not less than 1×10 7 CFU / mL.The microbial composition can be used for the banana wilt caused by fusarium oxysporum f. sp. Cubense tropical race 4.

The present application relates to the technical field of coffee processing, in particular to a coffee fresh fruit and fruit peel multi-strain synergistic fermentation equipment and process based on flavor guidance, comprising a fermentation tank main body, a stirring system, a temperature control system, a gas management system, a sensor integrated module, a multi-channel bacterial agent adding module and a control unit; the gas management system comprises an inert gas source, a sterile air source and a gas path control valve group for switching the gas path, which are communicated with the inside of the fermentation tank main body through pipelines; the multi-channel bacterial agent adding module comprises at least three independent bacterial agent storage tanks and a metering pump connected with the bottom discharge pipe of each bacterial agent storage tank. The present application can compound bacterial agents according to the preset viable count ratio through the cooperation of the three independent bacterial agent storage tanks and the high-precision metering pump; under the program driving of the control unit, the whole automatic switching fermentation process path from the establishment of the absolute anaerobic environment by nitrogen replacement to the switching of the sterile air and the maintenance of the set dissolved oxygen concentration can be completed by one key.

This invention relates to a compound microbial agent for the prevention and control of tobacco black shank disease, belonging to the field of biotechnology. The compound microbial agent comprises a suspension of *Pseudomonas aeruginosa* 232, *Bacillus cereus* 183, and *Mesophytes stolonifer* 161 in a volume ratio of 3:1:3, wherein the effective viable count in each of the aforementioned three bacterial suspensions is not less than 1 × 10⁻⁶. 9 CFU / mL. The compound biocontrol agent provided by this invention can effectively control tobacco black shank disease. Experimental verification shows that after 8 weeks of application of this agent to tobacco seedlings, the biocontrol effect against tobacco black shank disease can reach 33.34%.

The present application relates to the field of environmental microorganism and wastewater treatment technology, and specifically discloses a kind of fermentation microbial inoculum for sewage treatment and application.The fermentation microbial inoculum is made by immobilizing multifunctional composite microbial inoculum on chitosan magnetic microspheres;The multifunctional composite microbial inoculum is compounded by the fermentation broth of Pseudomonas koreensis, Lactobacillus acidophilus, Brevibacillus brevis, Timumasidium and Bacillus licheniformis according to a specific viable count ratio.The chitosan magnetic microspheres have Fe3O4 as magnetic core and are coated with crosslinked chitosan layer.The present application successfully constructs a microbial repair system with functional complementation and easy recovery by combining specific flora compounding with magnetic immobilization technology.The microbial inoculum can efficiently and synchronously remove nitrate, tetrabromobisphenol A and heavy metal multi-component pollutants in groundwater or contaminated water bodies, and can be quickly recovered and reused by external magnetic field, with the advantages of high treatment efficiency, environmental friendliness and low cost.

The invention belongs to the technical field of microbial technology and biomass resource utilization, and particularly relates to a degradation-resistant microbial agent for producing acetic acid by fermenting corn straw as well as a preparation method and application of the degradation-resistant microbial agent. The microbial inoculum is prepared by compounding monascus purpureus, wet cellulomonas and acetobacter pasteurianus and freeze-drying through a protective agent, the viable count is greater than or equal to 1.0 * 10 < 10 > CFUg <-1 >, the survival rate is greater than or equal to 95% after the microbial inoculum is stored at 4 DEG C for 180 days, and the microbial inoculum is excellent in cold-chain-free transportation adaptability. According to the microbial inoculum, full-link efficient conversion of cellulose-glucose-acetic acid and cellulose-glucose-ethanol-acetic acid is achieved through nonlinear synergistic metabolism of the three strains, corn straw is fermented for 120 hours under the intermittent perturbation stirring condition at the temperature of 35 DEG C, the degradation rate can reach 61.5%, and the yield of acetic acid can reach 24.7 gL <-1 > and is increased by 23.5% or above compared with the prior art.

The application relates to the field of feed additives, and particularly discloses a liquid feed additive containing bacillus and a preparation method thereof. The liquid feed additive comprises the following raw materials: culture solution, stabilizer, bacillus and acid adjusting solution. The addition amount of the stabilizer is 0.01-0.5 wt% based on the total amount of the culture solution. The stabilizer comprises at least two of xanthan gum, carrageenan, sodium carboxymethyl cellulose and guar gum. The addition amount of the bacillus is 2.5-5 g / L based on the culture solution. The culture solution is prepared through the following steps. Single bacterial colonies of the bacillus are inoculated into culture medium to obtain seed solution, the seed solution is sealed and cultured in a neutral environment, and the supernatant is obtained when the bacillus rate reaches more than 90% to obtain the culture solution. The obtained liquid feed additive is rich in probiotics, has good stability, and does not have phenomena such as stratification, precipitation and peculiar smell after long-time storage. The bacillus has high viable count, and can be directly fed to animals.

The invention relates to a microbial complex microbial inoculant and application thereof in a covering planting mode, and belongs to the technical field of microorganisms. The microbial synthetic flora provided by the invention comprises bacillus subtilis JZ23081 and bacillus velezensis JZ23083, wherein the ratio of effective viable counts of the two strains is 1: 1; the effective viable count of the complex microbial inoculant prepared from the synthetic flora is greater than or equal to 5.0 * 10 < 8 > / mL. The invention also provides application of the synthetic flora and the complex microbial inoculant in corn covering planting, and provides a product for promoting corn growth and a product for improving the soil nutrient conversion rate. The complex microbial inoculant can significantly promote the growth of corn in the seedling stage, improve the soil nutrient conversion rate and microbial activity, effectively solve the problems of late corn seedling emergence, low emergence rate, poor seedling growth and the like caused by slow straw decomposition and low ground temperature in northern early spring covering planting, and realize the synergistic interaction of strong corn seedlings and soil improvement. The method is especially suitable for spring corn production in a thin-layer black soil area, and has important practical significance on agricultural quality and efficiency improvement and ecological protection.

This invention discloses a microbial agent for regulating the microecology of aquatic bodies and its preparation method. The method includes: inoculating Rhodococcus erythropolis into a liquid culture medium, activating and expanding the culture at 28°C to obtain a Rhodococcus erythropolis fermentation broth with an effective viable count of 2.0×10⁹–2.8×10⁹ CFU / mL; then subjecting the broth to stepwise low-temperature adaptation at 15°C, 10°C, and 5°C to obtain a low-temperature adapted Rhodococcus erythropolis bacterial solution; culturing Bacillus glacialis and Bacillus belesii at 37°C and mixing them at a viable count ratio of 1:1 to obtain a Bacillus bacterial solution; subsequently, compounding the low-temperature adapted Rhodococcus erythropolis bacterial solution and the Bacillus bacterial solution at a viable count ratio of 1:(0.5–4), adding survival protection components and dispersion stabilizing components, and mixing thoroughly to obtain the microbial agent.

The invention discloses a microbial complex microbial inoculant for promoting the growth of leaf vegetables, which comprises bacillus subtilis, rhodopseudomonas palustris and bradyrhizobium japonicum, and the viable count ratio of the bacillus subtilis, the rhodopseudomonas palustris and the bradyrhizobium japonicum is 3: 1: 1. The microbial complex microbial inoculant for promoting the growth of the leaf vegetables comprises bacillus subtilis, rhodopseudomonas palustris and bradyrhizobium japonicum, and the viable count ratio of bacillus subtilis, rhodopseudomonas palustris and bradyrhizobium japonicum is 3: 1: 1. The preparation method of the microbial inoculum comprises the following steps: S1, respectively culturing the three bacteria to a logarithmic phase to obtain 109CFU / ml bacterial liquid; s2, mixing the fermentation broth according to a ratio, and ensuring that the viable count is accurately proportioned; s3, adding a carrier and auxiliary materials, and controlling the water content to be 35%; s4, drying at low temperature, protecting thallus activity, crushing, sieving and packaging to obtain a finished product. The three functional bacteria are scientifically proportioned, so that the synergistic growth promoting effect on the lettuce and the leaf mustard is realized, and the yield and the quality are remarkably improved.

The invention discloses a complex microbial inoculant adding method based on electroplating wastewater excess sludge backflow and application, and belongs to the technical field of wastewater biological treatment. The method specifically comprises the following steps: 1) adding a complex microbial inoculant into excess sludge to be refluxed to form mixed sludge; the complex microbial inoculant is formed by mixing a salt-tolerant strain and a functional strain in a viable count ratio of (1-2): (1-2); wherein halomonas is adopted as the salt-tolerant strain; sphingomonas paucimobilis is adopted as the functional strain; 2) introducing the mixed sludge into a sludge reflux system of a wastewater treatment device, staying for 0.5-8 hours, and performing contact and adaptation; and 3) returning the mixed sludge subjected to staying adaptation to a biological treatment reaction tank of the wastewater treatment device for wastewater treatment. According to the invention, the complex microbial inoculant is firstly added into the return sludge, so that the complex microbial inoculant is mixed and pre-adapted in the return process, and then enters the reaction tank, thereby realizing rapid colonization, uniform distribution and stable synergism, improving the electroplating wastewater treatment efficiency and shortening the system starting time.

The invention provides a functional microbial fertilizer for improving root rot resistance of crops and a preparation process thereof, and belongs to the technical field of microbial fertilizers. The bacterial fertilizer is prepared from 10-20 parts of a functional microorganism complex microbial inoculant, 30-50 parts of a modified carrier, 20-40 parts of a nutrition conditioner and 1-5 parts of a stabilizer, the complex microbial inoculant is prepared by compounding bacillus amyloliquefaciens J12, pseudomonas fluorescens Y8 and trichoderma harzianum Z3 according to the viable count ratio of 3: 2: 1; the modified carrier is a decomposed straw-humic acid-vermiculite composite carrier modified by chitosan hydrochloride. The preparation process adopts a step-by-step fermentation method to prepare the complex microbial inoculant, and combines the steps of modified carrier preparation and bacterial fertilizer composite aging. According to the invention, the efficient prevention and treatment of root rot are realized through the synergistic effect of strains, the modified carrier improves the stability of viable bacteria, the nutrition conditioner promotes the growth of crops, and the test shows that the prevention and treatment effect reaches 85% or more, the viable bacteria survival rate exceeds 55% after 90 days of storage, and the composition has both disease resistance and growth promotion, and has wide application prospects.