188 results about "Viable count" patented technology

The invention discloses a compound microbial fertilizer and application thereof in field planting. The survival rate of the paenibacillus mucilaginosus HX-02 disclosed by the invention can be up to 62.8% after 170d under the condition of 38% of nitrogen, phosphorus and potassium. The strain is organically combined with bacillus velezensis RKN1111 and a nitrogen-phosphorus-potassium inorganic fertilizer (N + P2O5 + K2O = 38%), the compound microbial fertilizer is developed, the viable count of effective bacteria is not less than 1.5 billion / g, and the viable count of paenibacillus mucilaginosus is not less than 0.5 billion / g. The compound microbial fertilizer taking the strain as a main functional bacterium is applied to field corn planting, can replace part of chemical fertilizers and increase soil fertility, can reduce the use amount of the chemical fertilizers by 15.56%, and achieves the effects of promoting the growth of field crops and increasing the yield by 10.2%. The paenibacillus mucilaginosus HX-02 is separated from soil, is harmless to human, livestock and environment, and meets the requirements of green plant protection and agricultural sustainable development.

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention relates to a saline-alkaline tolerant microbial seed coating agent and application thereof. The seed coating agent comprises a growth-promoting rhizobacteria inoculant and an auxiliary agent, the volume ratio of the growth-promoting rhizobacteria inoculant to the auxiliary agent is 1: (1-100); in each gram of the seed coating agent, the viable count of the growth-promoting rhizosphere bacteria is (1-100) * 10 < 10 > CFU; the growth-promoting rhizobacteria is selected from one or more of pseudomonas stutzeri A1501, pseudomonas fluorescens ATCC 13525, bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703, and the growth-promoting rhizobacteria is selected from one or more of bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703; on the basis of the total weight of the auxiliary agent, the auxiliary agent is prepared from the following components in percentage by weight: 1 to 9 percent of film forming agent, 1 to 9 percent of protective agent, 0.0001 to 0.001 percent of regulator, 0.0001 to 0.001 percent of active agent, 0 to 25 percent of filler and 58 to 97 percent of water. The seed coating agent can improve the rhizosphere colonization ability and survival rate of rhizosphere growth-promoting bacteria, can also improve the saline-alkaline tolerance of plant seeds, and effectively promotes plant growth.

The invention relates to a decomposition-promoting phosphorus-dissolving deodorizing microbial agent for composting and a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, paenibacillus zerzeri C-A and bacillus chestnut brown SC in a mass ratio of 1.5: (1-3): (1.5-4). The viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 1 * 10 < 9 > CFU / g. The preparation method comprises the following steps: (1) strain activation; (2) strain amplification culture; (3) bacterial powder preparation; and (4) strain compounding. The invention relates to application in clean composting of organic wastes. The invention has the following advantages: the three strains in the microbial inoculum do not generate antagonism, and can obviously accelerate the conversion of insoluble phosphorus, promote decomposition and improve the effect of removing NH3 and H2S.

The invention relates to the technical field of biocontrol microbial agents, and provides a preparation method of trichoderma particles. Comprising the following steps: inoculating and culturing trichoderma in a PDB culture medium, centrifugally collecting trichoderma thalli, embedding the trichoderma by using sodium alginate powder as a wall material, carrying out microencapsulation treatment on the trichoderma to obtain a microencapsulated trichoderma agent, drying the microencapsulated trichoderma agent at low temperature, and mixing and granulating an organic matter base fertilizer and the microencapsulated trichoderma agent to obtain the trichoderma particles. The trichoderma is subjected to microencapsulation treatment, so that the release time can be delayed, the influence on the activity of the trichoderma in the germination process of the coated corn seeds is avoided, and meanwhile, the trichoderma can be slowly released in the subsequent corn growth process to achieve a continuous biocontrol effect; the trichoderma particles prepared by the method are high in stability, a relatively high viable count of trichoderma can be still kept within a relatively long storage time, the storage time of the trichoderma particles is greatly prolonged, and the actually required long-time storage requirement is met.

The invention relates to a compound microorganism deodorization fungicide as well as a preparation method and application thereof, and belongs to the technical field of microorganism application. The invention provides a compound microorganism deodorization bacterium agent. The compound microorganism deodorization bacterium agent is prepared from bacillus cereus GXMU-Q5.3, streptomyces beihaiensis GXMU-J15 and lactobacillus paracasei GXMU-G1, the viable count ratio of the bacillus cereus GXMU-Q5.3 to the streptomyces beihaiensis GXMU-J15 to the lactobacillus paracasei GXMU-G1 is (1 to 2): (1 to 2): (1 to 2). The strain of the composite microbial deodorant agent is screened from soil and water at the stacking place of the marine product processing waste, the decomposition and deodorization effects on the marine product processing waste are obvious, the bacteria have a synergistic effect, and the deodorization effect of the composite microbial agent is improved by 40% or above compared with that of a single strain; the cost is low, the efficiency is high, the application range is wide, the deodorization period is short, and the safety is high.

The invention discloses bacillus subtilis and application thereof. The bacillus subtilis LDH-Y3 is obtained by being separated from Guangzhou commercially available Rakang goat milk pasteurized milk, and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 64442. The bacterial strain is high in growth speed and high in activity, the maximum viable count reaches 9.36 log CFU / mL, and the bacterial strain has good gastrointestinal tract digestion resistance. The bacillus subtilis is high in cellulase production capacity, CMCase (Carboxymethyl Cellulase) reaches 39.66 U / mL, beta-Gase (beta-Gase) reaches 2.36 U / mL, and Fpase (Fpasease) reaches 11.37 U / mL. The strain has the capability of inhibiting helminthosporium convex hilum and helminthosporium longibrachiatum, and after solid state fermentation of corn straw, the content of neutral detergent fibers in the corn straw can be reduced, and the digestibility of the straw is improved.

The invention relates to the technical field of bio-organic fertilizers, in particular to a preparation method and application of a bio-organic fertilizer based on secondary fermentation propagation of bacillus velezensis, and the preparation method comprises the following steps: uniformly mixing livestock and poultry manure and straw powder, adding a high-efficiency decay-promoting microbial inoculum and a compound additive, and carrying out trough type turning, stirring and mixing to obtain the bio-organic fertilizer. A compost material after primary fermentation is obtained; and after the water content of the compost material after primary fermentation is reduced to 45-55% and the temperature is reduced to 45 DEG C or below, adding an amino acid raw material and a bacillus velezensis inoculant, stirring and mixing, continuing fermentation, controlling the temperature of the compost body for secondary fermentation to be less than or equal to 60 DEG C, stirring once a day, and naturally airing after fermentation is completed, thereby obtaining the biological organic fertilizer. According to the technical scheme, the effective viable count in the biological organic fertilizer and the soil colonization effect of the biological organic fertilizer are improved, so that the prevention and control effect on continuous cropping obstacle related pathogenic fungal diseases is improved, and an efficient and reliable technical scheme is provided for biological prevention and control of continuous cropping obstacles of traditional Chinese medicinal materials and greenhouse vegetables.

The invention provides a method for preparing a bacterium-containing compound fertilizer and a method for increasing the viable count in the fertilizer. The method for preparing the bacterium-containing compound fertilizer comprises the following steps: spraying the surfaces of fertilizer particles with a liquid fungicide; performing first mixing treatment on the spraying treatment product and a first powder fungicide to obtain the bacterium-containing compound fertilizer; wherein the liquid fungicide comprises a second powder fungicide, biochemical potassium fulvate and water. Therefore, the method is simple in process and high in operability, and the addition amount of bacteria in the prepared bacteria-containing compound fertilizer and the retention rate of viable bacteria can be effectively increased by adopting the method.

The invention discloses a peptide for promoting proliferation of lactobacillus gasseri as well as a preparation method and application of the peptide. The peptide comprises any one or more amino acid sequences shown in SEQ ID NO.1-17. The molecular weight is 600-1400 Da, the glutamic acid content is 12.70%-48.91%, and the biological degree, the viable count and the nitrogen source utilization rate of the lactobacillus gasseri are remarkably improved. The strain proliferation promoting peptide is high in stability, simple in process and low in production cost, and the precise design theory of a high-density culture nitrogen source of lactobacillus gasseri is further improved; and scientific theoretical support is provided for design of an optimal culture medium and acquisition of high biomass through a fermentation engineering technology.

The invention discloses a microbial alliance for degrading acrylonitrile as well as a preparation method and application of the microbial alliance. The microbial alliance is composed of Burkholderia paragallinarum, Burkholderia zhejiangensis, Comamonas terrestris, Stenotrophomonas acidophilus and Trichoderma koningii. The ratio of the viable count of the burkholderia parakholderia to the viable count of the burkholderia zhejiang to the viable count of the comamonas terrestris to the viable count of the stenotrophomonas acidophilus to the viable count of the trichoderma koningii is 3: 1: 1: 1: 1. The microbial alliance has the advantages of stable flora structure, high acrylonitrile degradation efficiency, simple operation, low cost, no need of additional addition of other nutrients in the treatment process, and no secondary pollution, and belongs to an environment-friendly technology. The method has a wide application prospect in the field of treatment of acrylonitrile-containing wastewater (gas).

The invention discloses a preparation with functions of promoting growth of aquatic animals and improving water quality and application of the preparation. The preparation comprises compound bacterial powder and a traditional Chinese medicine extract in a mass ratio of (2-3): (1-2), the compound bacterial powder is bacillus velezensis J-1 bacterial powder and bifidobacterium G-1 bacterial powder in a mass ratio of (2-4): (3-6), the effective viable count of the bacillus velezensis J-1 bacterial powder is 1.5 * 10 < 9 >-2.5 * 10 < 9 > CFU / g, the effective viable count of the bifidobacterium G-1 bacterial powder is 1.0 * 10 < 10 >-1.5 * 10 < 10 > CFU / g, the bacillus velezensis J-1 and the bifidobacterium G-1 are both preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus velezensis J-1 bacterial powder and the bifidobacterium G-1 bacterial powder is CGMCC (China Center for Type Culture Collection). The preservation number of the bacillus velezensis J-1 is CCTCC (China Center for Type Culture Collection) No: M2021287, and the preservation number of the bifidobacterium G-1 is CCTCC No: M2024350; the traditional Chinese medicine extract is composite extract powder of rhizoma acori graminei and perilla leaves. According to the preparation, the synergistic interaction effect of the two bacteria is utilized, the effects of promoting growth of the aquatic animals and improving water quality can be remarkably improved, and meanwhile the effects of promoting growth of the aquatic animals and improving water quality can be further achieved by adding the compound traditional Chinese medicine extract of the rhizoma acori graminei and the perilla leaves.

The invention provides a microalgae biological synergist as well as a preparation method and application thereof, and belongs to the technical field of soil improvement and crop planting. The microalgae biological synergist is prepared from the following raw materials in percentage by weight: 35 to 50 percent of microalgae concentrated solution, 35 to 50 percent of liquid mineral source potassium fulvate and 15 to 30 percent of powdery bacteria, the microalgae are chlorella sorokiniana, and the cell density of the microalgae is (2.5 to 3.5) * 10 < 7 > cells / mL; the powdery bacterium is a composite functional flora composed of bacillus polymyxa, bacillus subtilis, bacillus cereus and bacillus licheniformis, and the effective viable count is greater than or equal to 2.0 billion / g. The biological synergist provided by the invention can effectively improve soil, and can greatly improve the drought resistance, water and fertilizer utilization efficiency, yield, quality and the like of crops.

The invention relates to a method for in-situ remediation or management and control of a petroleum contaminated site by micro-nano aeration enhanced microorganisms, which comprises the following steps: 1) injecting micro-nano bubbles into soil or underground water, and continuously aerating for 3-5 days for 6-10 hours every day; an air source of the micro-nano bubbles is one or a combination of two of air and oxygen; 2) 12-24 hours after the step 1) is completed, a compound microbial agent is injected into the soil or the underground water, the compound microbial agent comprises compound microorganisms, and the compound microorganisms comprise pseudomonas, leucas, phenyl bacilli and brevibacillus in the viable count ratio of (1-25): (1-15): (1-5): 1. According to the method, the energy consumption is low, secondary pollution is avoided, the remediation or control effect is excellent, and the total petroleum hydrocarbon (C10-C40) pollutant concentration of the contaminated site after remediation is remarkably reduced.

The invention provides a formula for regulating hyperglycemia by probiotics and a preparation method of the formula, and relates to the technical field of the probiotics, the formula comprises two formulas, the formula I comprises the probiotics, hypoglycemic pepper, raw malt, burdock, snow pear and the like, and the formula II comprises the probiotics, the hypoglycemic pepper, the raw malt, the burdock, the snow pear and the like; and the formula II consists of inonotuso bliquus polysaccharide, schizophyllum commune, chromium yeast, soybean peptide powder and lactobacillus fermentation product powder. The two formulas can be taken independently, alternately or jointly, and are used for regulating the blood sugar level of patients with hyperglycemia or type 2 diabetes mellitus. The probiotic powder contains a plurality of strains with high activity and high viable count, and plays a role in digesting and absorbing basic metabolism. The natural components in the formula have a hypoglycemic effect, and play a role in improving the secretion function of pancreas islet beta cells, improving insulin sensitivity, delaying absorption of carbohydrates, helping produce saliva and slake thirst, diluting blood sugar and other mechanisms.

The invention relates to the technical field of probiotic preparations, in particular to a process for preparing intestinal conditioning prebiotics from clostridium butyricum and radix astragali seu hedysari, which comprises the following steps: (1) multi-stage synergistic activation of clostridium butyricum; (2) carrying out composite enzymolysis on the astragalus extract; (3) symbiotic fermentation; (4) performing multi-layer embedding; the prepared prebiotics have a multi-dimensional intestine regulating mechanism, the bacteriostasis and mucous membrane repairing functions of the clostridium butyricum cooperate with the prebiotics effect and antioxidant activity of the astragalus polysaccharide to form a multi-protection system, and the intestinal flora imbalance is more effectively improved compared with single probiotics or prebiotics. The multi-layer embedding technology solves the problems of gastric acid inactivation and difficulty in intestinal colonization of probiotics, and spore induction of three-stage fermentation is matched, so that the viable count maintenance rate of the product is kept at a relatively high level after the product is stored for 6 months at normal temperature, and the shelf life is remarkably prolonged.

The invention relates to the technical field of biological agents, and particularly discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent provided by the invention comprises compound bacillus, a carrier and a coating agent, the composite bacillus comprises bacillus velezensis, bacillus atrophaeus and bacillus firmus with the viable count ratio of (3-7): (1-4): 2; the viable count of the compound microbial agent is greater than or equal to 5 * 10 < 9 > cfu / g; the invention also provides a preparation method of the compound microbial agent. The preparation method comprises the following steps: strain fermentation, carrier adsorption, granulation and enveloping and low-temperature drying. The compound microbial agent provided by the invention can improve the soil structure, enhance the soil fertility and inhibit soil-borne diseases, so that the growth condition of crops is improved, and the quality and yield of the crops are improved.

The invention relates to a compound probiotic formula, a preparation and application of the compound probiotic formula. The compound probiotic formula is prepared from bifidobacterium animalis HN019, bifidobacterium animalis Bb-12, bifidobacterium breve M-16V, lactobacillus rhamnosus HN001 and lactobacillus acidophilus NCFM. The ratio of the viable count of the bifidobacterium animalis HN019 to the viable count of the bifidobacterium animalis Bb-12 to the viable count of the bifidobacterium breve M-16V to the viable count of the lactobacillus rhamnosus HN001 to the viable count of the lactobacillus acidophilus NCFM is 2.5 to 1.2 to 1.3 to 2.5 to 2.5. The traditional Chinese medicine composition has the advantages of helping to establish intestinal flora balance, improving immunity, preventing infectious diseases, regulating immune balance, preventing and relieving eczema and allergy, bidirectionally regulating diarrhea and constipation, relieving gastrointestinal function discomfort symptoms, improving poor appetite, anorexia and dyspepsia, improving children crying and screaming at night and poor sleep, improving children growth and development retardation, and improving children immunity. Social emotion behaviors of children are improved, and attention deficits are improved; the probiotic composition can coexist and symbiotic, induces an organism to establish intestinal flora balance, relieves food allergy symptoms, and improves social emotion problems caused by food allergy.

The present disclosure relates to a method for efficiently producing lactic acid by fermenting brewers' spent grains. The method specifically includes the following steps: (1) adding water to brewers' spent grains after alkali pretreatment so as to adjust a solid content of a fermentation production system to 10%-15%, adding cellulase to the brewers' spent grains according to an addition amount of 5 FPU / g-10 FPU / g, carrying out hydrolysis at 50° C.-60° C. and pH of 4.5-5.2 for 20 hours-48 hours, and obtaining a prehydrolyzed substrate; and (2) inoculating hydrolysate prepared in step (1) with a lactic acid bacteria seed solution with a viable count of 1×108 CFU / mL-1×1010 CFU / mL according to an inoculation amount of 10%-15% (v / v), conducting fermentation at 40° C.-55° C. and pH of 6.0-7.2 for 72 hours-96 hours, and obtaining a fermentation broth.

The invention belongs to the technical field of biology, and particularly relates to a complex microbial inoculant for promoting formation of a biological membrane, a microcapsule and application of the complex microbial inoculant. The complex microbial inoculant comprises lactobacillus mucus fermentation FMBL L24016DMX with the preservation number of CCTCC NO: M20241583 and bifidobacterium longum subsp. Longum FMBL B241770LS with the preservation number of CCTCC NO: M 20241993, the complex microbial inoculant can grow on a culture medium containing prebiotics, the biofilm formation amount is the highest when the complex microbial inoculant is co-cultured in a culture medium containing isomaltooligosacharide, the complex microbial inoculant is prepared into a single-layer microcapsule or a double-layer microcapsule, and the complex microbial inoculant can be used for preparing the probiotics. The endophytic fungus has good embedding rate and viable count, can resist gastrointestinal environment, has good heat resistance, and has wide application prospects.

The invention relates to a preparation method of compound microorganism bacterium soil, microorganisms in the compound microorganism bacterium soil comprise bacillus subtilis YCBA0319 with the preservation number of CGMCC No.33006 and trichoderma longibrachiatum YCFA0522 with the preservation number of CGMCC No.41655. The preparation method comprises the following steps: selecting relatively clean clay or loam, and removing stones and plant residues; mixing the fine soil, the soybean meal and the cottonseed cake, stirring by using a double-shaft paddle type mixer, and spraying sterile water to adjust the water content; sub-packaging into polypropylene woven bags, sterilizing, and cooling for later use; preparing a seed solution of the bacillus subtilis YCBA0319; preparing a spore suspension of the trichoderma longibrachiatum YCFA0522; the bacillus subtilis is 2.5 * 10 < 9 > CFU / g, and the trichoderma longibrachiatum is 5.0 * 10 < 9 > CFU / g. According to the technical scheme, a granular structure is formed with soil in the fermentation process, so that an appropriate living microenvironment is provided for microorganisms, effective microbial communities are protected from being influenced by external adverse conditions, and the activity and function exertion of the effective microbial communities after field application are ensured.

The invention discloses a compound fermentation method of acid-resistant probiotics and application of the acid-resistant probiotics in blueberry juice, and particularly relates to compound fermentation of plant lactobacillus B4 and plant lactobacillus SS6 and application of the compound fermentation method. The two strains are preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the strains are respectively CGMCC NO.30881 and CGMCC NO.30880. The invention also discloses a preparation method of the strain. The viable count of the lactobacillus plantarum still exceeds 6 log CFU / ml after the lactobacillus plantarum is fermented for 72 hours in blueberry juice (pH = 3.0), and is two orders of magnitude higher than that of a common commercial strain (lactobacillus plantarum LP39); and the two strains are subjected to compound fermentation, so that the total phenol content, DPPH free radical scavenging rate and terpenoid content of the blueberry juice can be increased at the same time, and the influence of fermentation on the color of the blueberry juice can be reduced.

The invention discloses a high-density culture method, a fermentation process and application of plant lactobacillus CCFM8661, and belongs to the technical field of microorganisms. According to the invention, the culture medium is optimally designed and mainly comprises cane sugar, peptone, beef extract, yeast powder, Tween 80, magnesium sulfate heptahydrate, manganese sulfate monohydrate and Na2HPO4-citric acid buffer salt, and the components are optimized. According to the method, the growth rate and the viable count of the plant lactobacillus CCFM8661 are increased, the plant lactobacillus CCFM8661 enters a stable phase after being cultured for 21 hours, and the viable count of thalli of the obtained plant lactobacillus CCFM8661 reaches 1.5 * 10 < 12 > CFU / g. Compared with the original MRS culture medium, the OD600nm value is increased by 2.8 times. The plant lactobacillus CCFM8661 is not only suitable for food processing, but also can be applied to the fields of health care products and medicines, so that the culture medium disclosed by the invention has a wide market application prospect.

The invention discloses a haemophilus parasuis high-density fermentation culture medium as well as a culture method and application thereof, and relates to the technical field of microbial fermentation. The haemophilus parasuis high-density fermentation culture medium is prepared from the following components according to the content in 1L: 1.8 to 2g / L of glucose, 20 to 25g / L of organic nitrogen source, 2.0 to 3.0 g / L of dipotassium phosphate, 4.5 to 5.5 g / L of sodium chloride, 0.5 to 1.8 mg / L of NAD (Nicotinamide Adenine Dinucleotide), 8.2 to 8.6 mL / L of fetal calf serum and the balance of water, and the pH value is 7.0 to 7.5; wherein the organic nitrogen source is composed of Oxoid yeast and green source soybean peptone in a mass ratio of 1: 2. By optimizing the proportion of the glucose, the organic nitrogen source and the fetal calf serum, the viable count of the haemophilus parasuis is remarkably increased, when the culture medium is used for fermentation culture, OD600 can reach 1.8 through 7-hour fermentation, the viable count reaches 1010 CFU / mL, the viable count is increased by more than one time compared with that of a traditional culture medium, and the culture medium is suitable for large-scale production. And a high-density thallus raw material is provided for vaccine production.

The invention provides a food containing viable lactic acid bacteria, and aims to solve the problems of acidity increase and viable count reduction in the preservation process of foods with low lactic acid bacteria nutrient source (non-fat milk solid content and protein) content, such as lactic acid bacteria beverages and dairy product lactic acid bacteria beverages. The food contains a milk component and viable lactic acid bacteria, has a non-fat milk solid content of less than 8.0% by weight, a protein content of less than 2.7% by weight, and a pH of 4.6 or less, and contains an emulsifier and a water activity lowering agent.

The invention discloses a method for improving antioxidant activity of oil cakes through multi-strain fermentation combined with enzymolysis and application. The method comprises the following steps: adding a multi-strain mixed bacterium suspension into sterilized oil cakes, uniformly mixing, performing constant-temperature fermentation at 30 + / -2 DEG C, performing intermittent stirring in the fermentation process, and sequentially performing high-temperature sterilization and freeze-drying treatment after the fermentation is finished, the multi-strain mixed bacterium suspension is a mixed bacterium suspension of aspergillus niger, saccharomyces cerevisiae and bacillus subtilis, and the viable count ratio of the aspergillus niger to the saccharomyces cerevisiae to the bacillus subtilis in the multi-strain mixed bacterium suspension is (1-2): (1-2): (1-2). The application finds that a multi-strain composite fermentation combined enzymolysis technology can realize the synergistic interaction of fermentation and enzymolysis, obviously improves the antioxidant activity of the oil cake meal, and can be applied to preparation of cake meal feed.

The invention discloses a preparation method of whey peptone for promoting growth of bifidobacterium breve and improving freeze-drying survival rate, which belongs to the technical field of microorganisms, and comprises the following steps: hydrolyzing whey protein with papain and alkaline protease in sequence, collecting peptide liquid after passing through a ceramic membrane and ultrafiltration, and freeze-drying to prepare a target product whey peptone WPP, when the peptone is used as the only nitrogen source for culturing bifidobacterium breve, the growth of the bifidobacterium breve can be promoted, the freeze-drying survival rate of the bifidobacterium breve and the viable count of each gram of dry powder after freeze-drying are improved, and the application prospect is huge.

The invention belongs to the field of fermentation engineering, and particularly discloses a preparation method and application of edible fungus and probiotic co-culture fermentation liquor. A bidirectionally promoted edible fungus-probiotic symbiotic metabolism system is constructed by adopting a synchronous inoculation technology and combining with an optimized culture medium formula taking grain processing materials such as coarse rice powder as a main substrate. According to the method, in the culture period of 7-14 days, the fermentation liquor with the total triterpene content not lower than 35 mg / g, the total polysaccharide content not lower than 25 mg / g and the viable count of probiotics reaching 1 * 10 < 8 > CFU / mL or above can be obtained. The technology has the advantages of being easy and convenient to operate, low in cost and short in period, the content of the active ingredients of the edible fungi is remarkably increased while the viable count of the probiotics is maintained, and a new technical scheme is provided for developing high-quality functional food or preparations with the probiotic function and the active ingredients of the fungi.

The invention discloses a complex microbial inoculant for increasing the content of aroma-producing substances of barley bran as well as a preparation method and application of the complex microbial inoculant. The complex microbial inoculant is formed by compounding plant lactobacillus dy-1 and Enderbernella ferruginea Pro208 according to the viable count ratio of 5: 2. The preservation number of the plant lactobacillus dy-1 is CGMCC (China General Microbiological Culture Collection Center) No.6016; according to the present invention, the Feibian Escherichia coli (Pro208) has a preservation number of GDMCC 64830; the complex microbial inoculant can grow in a barley bran system, and the aroma and volatile substance composition of barley bran and the content of a key aroma-producing substance butyl acetate are remarkably improved; the complex microbial inoculant can utilize pyruvate dehydrogenase and esterifying enzyme to convert glucose into butyl acetate; in addition, the obtained complex microbial inoculant can be used for preparing the fermented barley bran beverage and remarkably improving the aroma of the fermented barley bran beverage, and has wide application prospects in the food industry.