144 results about "Viable count" patented technology

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention relates to the technical field of biocontrol microbial agents, and provides a preparation method of trichoderma particles. Comprising the following steps: inoculating and culturing trichoderma in a PDB culture medium, centrifugally collecting trichoderma thalli, embedding the trichoderma by using sodium alginate powder as a wall material, carrying out microencapsulation treatment on the trichoderma to obtain a microencapsulated trichoderma agent, drying the microencapsulated trichoderma agent at low temperature, and mixing and granulating an organic matter base fertilizer and the microencapsulated trichoderma agent to obtain the trichoderma particles. The trichoderma is subjected to microencapsulation treatment, so that the release time can be delayed, the influence on the activity of the trichoderma in the germination process of the coated corn seeds is avoided, and meanwhile, the trichoderma can be slowly released in the subsequent corn growth process to achieve a continuous biocontrol effect; the trichoderma particles prepared by the method are high in stability, a relatively high viable count of trichoderma can be still kept within a relatively long storage time, the storage time of the trichoderma particles is greatly prolonged, and the actually required long-time storage requirement is met.

The invention relates to the technical field of bio-organic fertilizers, in particular to a preparation method and application of a bio-organic fertilizer based on secondary fermentation propagation of bacillus velezensis, and the preparation method comprises the following steps: uniformly mixing livestock and poultry manure and straw powder, adding a high-efficiency decay-promoting microbial inoculum and a compound additive, and carrying out trough type turning, stirring and mixing to obtain the bio-organic fertilizer. A compost material after primary fermentation is obtained; and after the water content of the compost material after primary fermentation is reduced to 45-55% and the temperature is reduced to 45 DEG C or below, adding an amino acid raw material and a bacillus velezensis inoculant, stirring and mixing, continuing fermentation, controlling the temperature of the compost body for secondary fermentation to be less than or equal to 60 DEG C, stirring once a day, and naturally airing after fermentation is completed, thereby obtaining the biological organic fertilizer. According to the technical scheme, the effective viable count in the biological organic fertilizer and the soil colonization effect of the biological organic fertilizer are improved, so that the prevention and control effect on continuous cropping obstacle related pathogenic fungal diseases is improved, and an efficient and reliable technical scheme is provided for biological prevention and control of continuous cropping obstacles of traditional Chinese medicinal materials and greenhouse vegetables.

The invention provides a method for preparing a bacterium-containing compound fertilizer and a method for increasing the viable count in the fertilizer. The method for preparing the bacterium-containing compound fertilizer comprises the following steps: spraying the surfaces of fertilizer particles with a liquid fungicide; performing first mixing treatment on the spraying treatment product and a first powder fungicide to obtain the bacterium-containing compound fertilizer; wherein the liquid fungicide comprises a second powder fungicide, biochemical potassium fulvate and water. Therefore, the method is simple in process and high in operability, and the addition amount of bacteria in the prepared bacteria-containing compound fertilizer and the retention rate of viable bacteria can be effectively increased by adopting the method.

The invention discloses a preparation with functions of promoting growth of aquatic animals and improving water quality and application of the preparation. The preparation comprises compound bacterial powder and a traditional Chinese medicine extract in a mass ratio of (2-3): (1-2), the compound bacterial powder is bacillus velezensis J-1 bacterial powder and bifidobacterium G-1 bacterial powder in a mass ratio of (2-4): (3-6), the effective viable count of the bacillus velezensis J-1 bacterial powder is 1.5 * 10 < 9 >-2.5 * 10 < 9 > CFU / g, the effective viable count of the bifidobacterium G-1 bacterial powder is 1.0 * 10 < 10 >-1.5 * 10 < 10 > CFU / g, the bacillus velezensis J-1 and the bifidobacterium G-1 are both preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus velezensis J-1 bacterial powder and the bifidobacterium G-1 bacterial powder is CGMCC (China Center for Type Culture Collection). The preservation number of the bacillus velezensis J-1 is CCTCC (China Center for Type Culture Collection) No: M2021287, and the preservation number of the bifidobacterium G-1 is CCTCC No: M2024350; the traditional Chinese medicine extract is composite extract powder of rhizoma acori graminei and perilla leaves. According to the preparation, the synergistic interaction effect of the two bacteria is utilized, the effects of promoting growth of the aquatic animals and improving water quality can be remarkably improved, and meanwhile the effects of promoting growth of the aquatic animals and improving water quality can be further achieved by adding the compound traditional Chinese medicine extract of the rhizoma acori graminei and the perilla leaves.

The invention relates to a method for in-situ remediation or management and control of a petroleum contaminated site by micro-nano aeration enhanced microorganisms, which comprises the following steps: 1) injecting micro-nano bubbles into soil or underground water, and continuously aerating for 3-5 days for 6-10 hours every day; an air source of the micro-nano bubbles is one or a combination of two of air and oxygen; 2) 12-24 hours after the step 1) is completed, a compound microbial agent is injected into the soil or the underground water, the compound microbial agent comprises compound microorganisms, and the compound microorganisms comprise pseudomonas, leucas, phenyl bacilli and brevibacillus in the viable count ratio of (1-25): (1-15): (1-5): 1. According to the method, the energy consumption is low, secondary pollution is avoided, the remediation or control effect is excellent, and the total petroleum hydrocarbon (C10-C40) pollutant concentration of the contaminated site after remediation is remarkably reduced.

The invention relates to the technical field of probiotic preparations, in particular to a process for preparing intestinal conditioning prebiotics from clostridium butyricum and radix astragali seu hedysari, which comprises the following steps: (1) multi-stage synergistic activation of clostridium butyricum; (2) carrying out composite enzymolysis on the astragalus extract; (3) symbiotic fermentation; (4) performing multi-layer embedding; the prepared prebiotics have a multi-dimensional intestine regulating mechanism, the bacteriostasis and mucous membrane repairing functions of the clostridium butyricum cooperate with the prebiotics effect and antioxidant activity of the astragalus polysaccharide to form a multi-protection system, and the intestinal flora imbalance is more effectively improved compared with single probiotics or prebiotics. The multi-layer embedding technology solves the problems of gastric acid inactivation and difficulty in intestinal colonization of probiotics, and spore induction of three-stage fermentation is matched, so that the viable count maintenance rate of the product is kept at a relatively high level after the product is stored for 6 months at normal temperature, and the shelf life is remarkably prolonged.

The invention relates to the technical field of biological agents, and particularly discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent provided by the invention comprises compound bacillus, a carrier and a coating agent, the composite bacillus comprises bacillus velezensis, bacillus atrophaeus and bacillus firmus with the viable count ratio of (3-7): (1-4): 2; the viable count of the compound microbial agent is greater than or equal to 5 * 10 < 9 > cfu / g; the invention also provides a preparation method of the compound microbial agent. The preparation method comprises the following steps: strain fermentation, carrier adsorption, granulation and enveloping and low-temperature drying. The compound microbial agent provided by the invention can improve the soil structure, enhance the soil fertility and inhibit soil-borne diseases, so that the growth condition of crops is improved, and the quality and yield of the crops are improved.

The invention belongs to the technical field of biology, and particularly relates to a complex microbial inoculant for promoting formation of a biological membrane, a microcapsule and application of the complex microbial inoculant. The complex microbial inoculant comprises lactobacillus mucus fermentation FMBL L24016DMX with the preservation number of CCTCC NO: M20241583 and bifidobacterium longum subsp. Longum FMBL B241770LS with the preservation number of CCTCC NO: M 20241993, the complex microbial inoculant can grow on a culture medium containing prebiotics, the biofilm formation amount is the highest when the complex microbial inoculant is co-cultured in a culture medium containing isomaltooligosacharide, the complex microbial inoculant is prepared into a single-layer microcapsule or a double-layer microcapsule, and the complex microbial inoculant can be used for preparing the probiotics. The endophytic fungus has good embedding rate and viable count, can resist gastrointestinal environment, has good heat resistance, and has wide application prospects.

The invention relates to a preparation method of compound microorganism bacterium soil, microorganisms in the compound microorganism bacterium soil comprise bacillus subtilis YCBA0319 with the preservation number of CGMCC No.33006 and trichoderma longibrachiatum YCFA0522 with the preservation number of CGMCC No.41655. The preparation method comprises the following steps: selecting relatively clean clay or loam, and removing stones and plant residues; mixing the fine soil, the soybean meal and the cottonseed cake, stirring by using a double-shaft paddle type mixer, and spraying sterile water to adjust the water content; sub-packaging into polypropylene woven bags, sterilizing, and cooling for later use; preparing a seed solution of the bacillus subtilis YCBA0319; preparing a spore suspension of the trichoderma longibrachiatum YCFA0522; the bacillus subtilis is 2.5 * 10 < 9 > CFU / g, and the trichoderma longibrachiatum is 5.0 * 10 < 9 > CFU / g. According to the technical scheme, a granular structure is formed with soil in the fermentation process, so that an appropriate living microenvironment is provided for microorganisms, effective microbial communities are protected from being influenced by external adverse conditions, and the activity and function exertion of the effective microbial communities after field application are ensured.

The invention discloses a compound fermentation method of acid-resistant probiotics and application of the acid-resistant probiotics in blueberry juice, and particularly relates to compound fermentation of plant lactobacillus B4 and plant lactobacillus SS6 and application of the compound fermentation method. The two strains are preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the strains are respectively CGMCC NO.30881 and CGMCC NO.30880. The invention also discloses a preparation method of the strain. The viable count of the lactobacillus plantarum still exceeds 6 log CFU / ml after the lactobacillus plantarum is fermented for 72 hours in blueberry juice (pH = 3.0), and is two orders of magnitude higher than that of a common commercial strain (lactobacillus plantarum LP39); and the two strains are subjected to compound fermentation, so that the total phenol content, DPPH free radical scavenging rate and terpenoid content of the blueberry juice can be increased at the same time, and the influence of fermentation on the color of the blueberry juice can be reduced.

The invention discloses a haemophilus parasuis high-density fermentation culture medium as well as a culture method and application thereof, and relates to the technical field of microbial fermentation. The haemophilus parasuis high-density fermentation culture medium is prepared from the following components according to the content in 1L: 1.8 to 2g / L of glucose, 20 to 25g / L of organic nitrogen source, 2.0 to 3.0 g / L of dipotassium phosphate, 4.5 to 5.5 g / L of sodium chloride, 0.5 to 1.8 mg / L of NAD (Nicotinamide Adenine Dinucleotide), 8.2 to 8.6 mL / L of fetal calf serum and the balance of water, and the pH value is 7.0 to 7.5; wherein the organic nitrogen source is composed of Oxoid yeast and green source soybean peptone in a mass ratio of 1: 2. By optimizing the proportion of the glucose, the organic nitrogen source and the fetal calf serum, the viable count of the haemophilus parasuis is remarkably increased, when the culture medium is used for fermentation culture, OD600 can reach 1.8 through 7-hour fermentation, the viable count reaches 1010 CFU / mL, the viable count is increased by more than one time compared with that of a traditional culture medium, and the culture medium is suitable for large-scale production. And a high-density thallus raw material is provided for vaccine production.

The invention provides a food containing viable lactic acid bacteria, and aims to solve the problems of acidity increase and viable count reduction in the preservation process of foods with low lactic acid bacteria nutrient source (non-fat milk solid content and protein) content, such as lactic acid bacteria beverages and dairy product lactic acid bacteria beverages. The food contains a milk component and viable lactic acid bacteria, has a non-fat milk solid content of less than 8.0% by weight, a protein content of less than 2.7% by weight, and a pH of 4.6 or less, and contains an emulsifier and a water activity lowering agent.

The invention discloses a method for improving antioxidant activity of oil cakes through multi-strain fermentation combined with enzymolysis and application. The method comprises the following steps: adding a multi-strain mixed bacterium suspension into sterilized oil cakes, uniformly mixing, performing constant-temperature fermentation at 30 + / -2 DEG C, performing intermittent stirring in the fermentation process, and sequentially performing high-temperature sterilization and freeze-drying treatment after the fermentation is finished, the multi-strain mixed bacterium suspension is a mixed bacterium suspension of aspergillus niger, saccharomyces cerevisiae and bacillus subtilis, and the viable count ratio of the aspergillus niger to the saccharomyces cerevisiae to the bacillus subtilis in the multi-strain mixed bacterium suspension is (1-2): (1-2): (1-2). The application finds that a multi-strain composite fermentation combined enzymolysis technology can realize the synergistic interaction of fermentation and enzymolysis, obviously improves the antioxidant activity of the oil cake meal, and can be applied to preparation of cake meal feed.

The invention discloses a preparation method of whey peptone for promoting growth of bifidobacterium breve and improving freeze-drying survival rate, which belongs to the technical field of microorganisms, and comprises the following steps: hydrolyzing whey protein with papain and alkaline protease in sequence, collecting peptide liquid after passing through a ceramic membrane and ultrafiltration, and freeze-drying to prepare a target product whey peptone WPP, when the peptone is used as the only nitrogen source for culturing bifidobacterium breve, the growth of the bifidobacterium breve can be promoted, the freeze-drying survival rate of the bifidobacterium breve and the viable count of each gram of dry powder after freeze-drying are improved, and the application prospect is huge.

The invention belongs to the field of fermentation engineering, and particularly discloses a preparation method and application of edible fungus and probiotic co-culture fermentation liquor. A bidirectionally promoted edible fungus-probiotic symbiotic metabolism system is constructed by adopting a synchronous inoculation technology and combining with an optimized culture medium formula taking grain processing materials such as coarse rice powder as a main substrate. According to the method, in the culture period of 7-14 days, the fermentation liquor with the total triterpene content not lower than 35 mg / g, the total polysaccharide content not lower than 25 mg / g and the viable count of probiotics reaching 1 * 10 < 8 > CFU / mL or above can be obtained. The technology has the advantages of being easy and convenient to operate, low in cost and short in period, the content of the active ingredients of the edible fungi is remarkably increased while the viable count of the probiotics is maintained, and a new technical scheme is provided for developing high-quality functional food or preparations with the probiotic function and the active ingredients of the fungi.

The invention relates to the technical field of microbial preparations for agricultural environment restoration, and provides a microbial agent for reducing pesticide residues in soil, which is characterized by comprising the following components in parts by weight: 30-60 parts of ochrobactrum having viable count of more than or equal to 5 * 10 < 8 > cfu / g; the spore number is greater than or equal to 2 * 10 < 8 > cfu / g. The final residues of imidacloprid and difenoconazole in the compounded microbial agent are reduced to about 0.20 mg / kg, balanced and efficient removal is realized, and the possible unbalance situation of'degrading one pesticide and remaining one pesticide 'in the traditional method is avoided.

The application provides a preparation method of an antioxidant Aeschynomene indica L. probiotic fermentation liquor, and belongs to the technical field of biological fermentation and microbial agents. The method comprises the following steps: step one, respectively preparing an Aeschynomene indica L. extract and a probiotic seed liquor; and step two, inoculating the seed liquor into the Aeschynomene indica L. extract or a culture medium containing the Aeschynomene indica L. extract for fermentation to obtain the Aeschynomene indica L. probiotic fermentation liquor. The Aeschynomene indica L. probiotic fermentation liquor provided by the application can effectively maintain the viable count of probiotics so that the probiotics can exert the probiotic effect, and the Aeschynomene indica L. water extract itself also has multiple probiotic activities, and the content of antioxidant metabolites is obviously increased after fermentation.

The application belongs to the technical field of microorganism detection, and particularly relates to a bifidobacterium viable count detection culture medium, a detection method and application. The application provides a culture medium for detecting bifidobacterium, wherein the culture medium comprises MRS culture medium and an antibiotic solution; the antibiotic solution comprises streptomycin, kanamycin and sodium propionate. The culture medium can ensure normal growth of bifidobacterium, has strong inhibition on lactobacillus, can effectively realize accurate detection of bifidobacterium, and avoids interference of lactobacillus.

The invention relates to the technical field of probiotic embedding, in particular to a probiotic microcapsule as well as a preparation method and application thereof. The probiotic microcapsule comprises probiotics and a composite wall material used for embedding the probiotics, the probiotics are probiotics with negative charges, and the composite wall material is composed of carboxymethyl pachymaran and soybean protein isolate. The probiotic microcapsule provided by the embodiment of the invention has significantly improved survival rate in gastrointestinal tracts and under low-temperature and normal-temperature storage conditions, has high stability and is suitable for freeze drying, and the final viable count exceeds 10 < 6 > CFU / g after gastrointestinal digestion and long-term storage, so that the probiotic microcapsule reaches the standard of probiotic food, and has good application prospects. The method has important research value and market prospect.

The present application provides a kind of wettable powder and its preparation method and application, belong to forest disease biological control technical field.The wettable powder of the present application is made of the following mass fraction components: Bacillus subtilis WB1 fermentation liquor 90-110 parts, filler 42-48 parts, auxiliary 28-32 parts.The wettable powder of Bacillus subtilis WB1 prepared by the present application has high viable count, good suspensibility, dispersibility and wettability, can be stored, transported and sprayed in large area in forest at room temperature for a long time, has broad-spectrum antibacterial property, and has good control effect on walnut bacterial black spot.The preparation method of the wettable powder of the present application is simple, low in cost, and suitable for large-scale popularization and application.

The invention provides an efficient microbial fermentation inoculant for straws and a preparation method thereof, and relates to the technical field of biodegradation. The efficient microbial fermentation inoculant for the straw is formed by compounding four strains including trichoderma reesei, streptomycete, lactobacillus plantarum and bacillus amyloliquefaciens according to the mass ratio, the ratio of the viable count of the four strains is that the ratio of the viable count of the trichoderma reesei to the viable count of the streptomycete to the viable count of the lactobacillus plantarum to the viable count of the bacillus amyloliquefaciens is 3: 2: 2: 1.5, the total viable count of the inoculant is larger than or equal to 1.3 * 10 CFU / g, and the cellulase activity is larger than or equal to 85 U / g. The laccase activity is greater than or equal to 42U / g. The invention can effectively solve the industrial pain points of difficult degradation of straws, poor quality and weak stability, and in agricultural production, the microbial inoculum can increase the content of organic matters in soil, can reduce the feed cost in the feed field, and can be used for production of straw-based organic fertilizers in the environmental protection field, and can reduce the use amount of chemical fertilizers.

The invention relates to a harmless treatment method for solid waste. The compound probiotic microbial agent comprises bacillus licheniformis, lactobacillus rimosii, lactobacillus plantarum, clostridium butyricum and saccharomycetes, the effective viable count is larger than or equal to 0.5 billion / mL, pig manure, corn straw and saw dust are mixed according to the mass ratio to obtain a compost raw material, the initial carbon-nitrogen ratio (C / N) is adjusted to be 25-35, the water content is adjusted to be 55-65%, and the compost raw material is fermented to obtain the compound probiotic microbial agent. And adding a composite probiotic inoculant accounting for 0.5-2.5% of the total mass into the compost raw material. Through probiotic combination and concentration optimization, the problems of decomposition and pollution of various ARGs are solved at the same time.

This invention discloses a composite microbial agent for improving saline-alkali soil, its preparation method, and its application. The composite microbial agent is composed of *Frederick's chamaejasminoides* and *Zoococcus halophilus* with a viable count ratio of (0.2–2):1. Research shows that, under the same total viable count conditions, the composite microbial agent exhibits significantly higher salt tolerance, IAA production, siderophore production capacity, ACC deaminase activity, phosphorus solubility, nitrogen fixation capacity, and extracellular polysaccharide production compared to single-strain agents, demonstrating a significant synergistic effect. The composite agent can also be combined with the salt-tolerant plant *Arundo donax* to construct a synergistic remediation system for saline-alkali land. This system significantly reduces the pH and sodium ion content of saline-alkali soil, improves soil nutrient availability, and significantly promotes *Arundo donax* growth and biomass accumulation, providing an innovative technical solution for rapid, efficient, and sustainable ecological restoration and agricultural utilization of saline-alkali land.

This invention discloses *Bacillus licheniformis* strain LWY-37-2, which was deposited on February 21, 2023, at the China General Microbiological Culture Collection Center (CGMCC), located in Beijing, with accession number CGMCC No. 26654. This invention also discloses a method for degrading condensed tannins using *Bacillus licheniformis* LWY-37-2, comprising the following steps: setting an inoculation viable count of 5 × 10⁻⁶ cells / year. 6 With a CFU / g content and a feed-to-water ratio of 1:0.8, the pH of the rapeseed meal fermentation substrate was adjusted to 7 without sterilization. Fermentation was carried out at 30℃ for 3 days, with the substrate turned over every 12 hours. The Bacillus licheniformis LWY-37-2 disclosed in this invention shows excellent application prospects in fermented plant-based feeds.

The present invention discloses a fly larvae killer. The fly larvae killer comprises the following substances by weight percentage: 5-10% of S-methoprene, 5-10% of Bacillus, and the remainder of auxiliary materials; wherein the Bacillus includes Bacillus subtilis and Bacillus licheniformis, and the total effective viable count thereof is ≥2×10 10 CFU / g. This fly larvae killer has a clear effect, is easy to use, leaves no residue in the animal body, does not produce harmful metabolites, is green and environmentally friendly, and is of great significance to improving the ecological environment of farms.

This invention relates to the field of functional microbial screening and application technology, specifically providing a probiotic crystal ball and its preparation method. The probiotic crystal ball consists of three parts: a crystal nucleus, a crystal mantle, and a crystal shell. The crystal nucleus contains *Lactobacillus plantarum* powder. The *Lactobacillus plantarum* strain is VHProbi R14, with accession number CCTCC NO: M2023721. This strain can effectively regulate the intestinal flora and significantly increase the abundance of beneficial *Akermansia* species, especially *Akermansia myxophilus* (Akk bacteria). The probiotic crystal ball provided by this invention has a two-layer protective structure, exhibiting excellent stability and heat resistance. It can effectively maintain the viable count of *Lactobacillus plantarum* VHProbi R14 during transportation, storage, and processing, significantly improving the applicability of *Lactobacillus plantarum* VHProbi R14, and can be widely used in food, health products, and other fields.

This invention discloses a *Lactobacillus plantarum* bacterial powder, its preparation method, and its application. The wall material of the bacterial powder includes resistant starch, whey protein, and a heat protectant. The bacterial cells in the powder are *Lactobacillus plantarum* TH103. The mass percentages of resistant starch, whey protein, and the heat protectant are 7-23%, with the total mass percentage of resistant starch and whey protein being 3-15% and the mass percentage of the heat protectant being 4-8%. The viable count of the bacterial powder is (3.5-9.0) × 10⁻⁶. 9 The CFU / g and moisture content are 5-7%. This invention uses resistant starch and whey protein as protective carriers, supplemented with heat-resistant protective agents, to achieve the preparation of *Lactobacillus plantarum* powder by spray drying. The obtained *Lactobacillus plantarum* powder has a viable count and moisture content that meet the relevant requirements for probiotic foods, and has good powder properties. It can be directly brewed in powder form or applied to fermented foods such as yogurt.

The invention belongs to the technical field of quantitative detection of microorganisms, and particularly relates to a method for quantitatively detecting the viable count of mycoplasma bovis. The method for quantitatively detecting the viable count of the mycoplasma bovis is finally obtained by exploring the linear relation between the protein concentration and the viable count of multiple batches of mycoplasma bovis liquid samples after optimization of experimental conditions such as bacterium liquid preservation conditions, ultrasonic crushing modes, crushing duration and sample treatment modes, and the method is good in stability, high in timeliness and high in accuracy. The detection result is accurate, and a convenient and effective method is provided for rapid detection of the viable count of the mycoplasma bovis liquid.