1218 results about "Viable count" patented technology

The invention discloses a hametz used for fermenting tobacco and applications thereof. The hametz is characterized by comprising three strains of Bacillus subtilis, lactobacillus and microzyme, the viable count ratio of which is 10 to 50: 2 to 30: 2 to 30. The hametz can ferment waste material of the tobacco or the flavor used for producing natural cigarettes by using the tobacco and can be used for tobacco fermentation. The flavor used for the natural cigarettes prepared by the hametz has a velvet fragrance and can remarkably increase the fragrance, reduce the unpurified gas and reduce the thrill as well when the flavor is used in the cigarettes, thus leading the smoke gas to be lingering and smooth. The hametz used for fermenting tobacco leaves can remarkably shorten the alcoholizing time and improve the quality of the tobacco leaves.

The invention discloses a soil heavy mental repairing agent and a preparation method and an application thereof. The soil heavy mental repairing agent is composed of an organic material, a mineral raw material, a carbon material, an inorganic fertilizer and a microbial agent, wherein the viable count of the microbial agent is 1*10<7>-1*10<10>, the content of N, P2O5 and K2O is 8%-15% of the content of total nutrient, the content of the organic material is 20%-40% and pH is 5.5-8.5. The invention also provides an application method of the soil heavy mental repairing agent. The dosage is 80-150kg/mu; the effective heavy metal content of the soil can be effectively reduced; the heavy metal absorption of the crop can be obviously restrained; the crop yield and quality can be improved; the yield and income can be increased; the soil heavy mental repairing agent has significance in promoting social stability, economic growth and environmental diplomacy and guaranteeing ecological safety, food safety and human health.

A full nutrition fertilizer of biology type and its producing method belongs to organic complex fertilizer field in biology type. The components of the full nutrition fertilizer is 20-50% of organic waste liquid, 10-30% of urea, 8-15% of diammomium phosphate, 10-25% of potassium sulfate, 5-20% of medium trace element, 1-5% of microbial inoculum, 0-0.2% of root-promoting agent; 0-2% of solutizer, effective viable count content of the microbial inoculum of more than 1010/g. The fertilizer is produced by adding the urea, diammomium phosphate, potassium sulfate, medium trace element, root-promoting agent, solutizer into organic waste liquid and blending uniformly, then adding microbial inoculum. The invention uses the wastes to produce fertilizer with a low cost a high availability and a good prospect in the fertilizer industry. The fertilizer can be widely applied to the vegetables, melon and fruits, food, tobacco, forests and different commercial crops.

The invention relates to a method for preparing health-benefiting fermented soybean meal enriched in small peptides, gamma-aminobutyric acid (GABA), reducing sugar, lactobacillus plantarum and antimicrobial peptide. By utilizing strains of aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum, the small peptides, free amino acids, reducing sugar, GABA and high viable count content in the lactobacillus plantarum in the health-benefiting fermented soybean meal are obtained through the two-stage solid state fermentation method, the small peptides account for 8.01-18 percent, the free amino acids has the content of 80-300mg/g, the reducing sugar reaches 20-80mg/g, the GABA reaches 0.317-0.5mg/g, the viable count content in the lactobacillus plantarum is 2-6.5*1010cfu/g, the antibacterial titer of antimicrobial peptides is 2.0*103-7.0*104 IU/g, and the yield of the health-benefiting fermented soybean meal is 82.03-90 percent. According to the process, the nutrient effect of the soybean meal can be improved, the prepared fermented soybean meal has the antibacterial effect, partial antibiotics can be replaced, the fermented soybean meal is conveniently preserved and saved, the process is simple and feasible, and the fermented soybean meal is green and environment-friendly and has application significance and wide prospect in the feed industry and breeding industry.

The invention provides a special bacterial manure for peanut planting, which comprises the following components in parts by weight: 5-25 parts of a compound microbial agent, 30-65 parts of dreg composite powder, 5-20 parts of starch and 10-25 parts of bentonite, wherein the compound microbial agent is composed of bacillus subtilis agent, a rhodopseudomonas palustris agent, a trichoderma harzianum agent and a paecilomyces lilacinus agent; the mass ratio of the bacillus subtilis agent to the rhodopseudomonas palustris agent to the trichoderma harzianum agent to the paecilomyces lilacinus agent is 5:2:1:2, and the total effective viable count of the compound microbial agent is10 billion cfu/g. The bacterial manure is mainly used as a base fertilizer, and can change the microenvironment around the roots of peanuts to prevent various plant diseases and insect pests while promoting nutrient absorption of the peanuts, so that the yield and quality of the peanuts are improved.

The invention provides functional soy peptide fermented milk and a preparation method thereof and relates to a manufacturing method of fermented dairy products in the field of food processing. The method is carried out according to the following steps: (1) the preparation of fermented base stock; (2) the preparation of mother cultures; (3) synchronous enzymolysis and fermentation; (4) blending and homogenizing; (5) ultrahigh pressure sterilization and filling; by adopting the steps, a finished product is obtained. The method adopts protease and lactic acid bacteria synchronous-enzymolysis fermentation technology, and ultrasonic waves are adopted to process slurry-residue mixture which is ground, so as to increase the dissolving-out rate of the protein and prepare acidity soy-bean milk by ultrahigh pressure and non-heat processing. The product taste is coordinated without enzymolysis odour; the enzymolysis fermentation time can shorten to 2-4h, and the production cost is low. The ultrahigh pressure processing can remarkably improve the product taste; the method can keep and produce new functional peptide, the lactic acid bacteria is inhibited to ferment continuously, and a certain micro-organism viable counts are kept; the shelf life of the acidity soy-bean milk is prolonged, so as to prevent the acid milk from being whey-separated owning to continuous increasing of acidity; inaddition, the product can be stored and marketed at normal temperature.

The invention relates to a method for preparing an enzyme and bacterium composite agent for treating sewage and sludge, which comprises the following steps: (1) preparing bacillus subtilis powder; (2) preparing bacterial solution of photosynthetic bacteria; (3) obtaining indigenous bacteria by collection, culture and enrichment culture; (4) performing domestication culture of working bacteria, namely adding indigenous bacteria and a composite culture medium according to different sludge loads, adding bacillus subtilis enzyme powder in an amount which is 0.1 to 0.4 percent based on total weight and bacterial solution of photosynthetic bacteria in an amount which is 1 to 3 percent based on the total weight, performing tests under different loads and different organic matter concentration, determining an optimal parameter, and repeating for 3 times; and (5) performing domestication and amplification culture of the working bacterium composite agent, and obtaining the domesticated working bacterium composite agent in which the viable count per milliliter is cfu 10<7> to 10<9>. The enzyme and bacterium composite agent prepared by the method can treat sewage and sludge efficiency, simplyand conveniently, has very high adaptability to any sewage environment, and has a guiding and coordinating effect on beneficial decomposing bacteria in local environments.

The invention discloses direct vat set Lactobacillus casei subsp. rhamnosus freeze-dried powder and a preparation method thereof. The method comprises the following steps: propagating Lactobacillus casei subsp. rhamnosus by a two-step method to make cells in a synchronous growth period as possible; and in a production culture medium, controlling pH to between 6.0 and 6.8 with NaOH solution on line, culturing for 16 to 24h at a temperature of between 35 and 37 DEG C, centrifugating, collecting bacterial sludge, adding protective agent solution, pre-freezing for 0.5 to 2.0h at a temperature of between -40 and -65 DEG C, making the thickness of bacteria on the wall of a container between 5 and 15 mm, and drying to prepare the direct vat set freeze-dried powder. The viable count of the freeze-dried powder reaches between 10 and 10cfu/g; and the freeze-dried powder has the characteristics of good rehydration, good luster, good fluidity, easy storage and transportation, convenient production and the like, and can be directly used for fermentation production of pickles, sausages, acidophilus milk, L-lactic acid, corn flour, bean meal and peanut meal, and used as a probiotic preparation and the like.

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.

The present invention discloses a compound microbial feed additive. It is a compound microbial feed additive obtain by adding 7 normal microbial pools into solid culture medium and making solid fermentation, drying and pulverizing to obtani powered material. Said 7 liquid micriobial pools are bacillus cereus, peptococcus, lactic acid bacteria, lactobacillus bifidus, yeast, photosynthetic bacteria and actionmycete. In said invented feed additive its viable count is greater than or equal to 180 handred million/gand total number of bacteria is greater than or equal to 240 handred milling/g. Said additive can be aded into animal feed, and can reduce fed-meat ratio and can raise disease resistance of animal.

The invention provides potato-bread frozen dough and a preparation method thereof. The potato-bread frozen dough comprises the following components: potato powder and wheat flour, wherein the weight rate of the potato powder to the wheat flour is (10-40): (90-60). The preparation method comprises the following steps of: mixing the potato powder and the wheat flour, and adding water as well as an appropriate amount of yeast, sugar, salt, butter, eggs and the like into the obtained mixture so as to obtain dough; and then, after carrying out partitioning, forming and pre-fermenting on the dough, freezing the dough and then taking out the dough to carry out packing, and carrying out freezing storage on the dough. The potato-bread frozen dough (the viable count of yeasts in the frozen quality guarantee period is greater than or equal to 107 cfu/g) produced by using the method disclosed by the invention can be subjected to unfreezing, re-fermenting and baking so as to prepare fresh bread as required, and the technique facilitates the production management of bread and the freshness preservation of products; in addition, due to the addition of the potato powder, the nutritive value of bread is improved, the texture and taste of bread are improved, and the comprehensive utilization and development of potato processed by-products are facilitated, thereby having great significance in improving the additional values thereof.

The invention relates to a manioc waste biological feed additive for pigs, and the manioc waste biological feed additive is prepared from the following raw materials by weight percent: 50%-55% of manioc waste, 30%-35% of straw powder, 10%-14% of soybean meal and 1% of microbial strain. The manioc waste biological feed additive for the pigs has the following technical effects: as for the microbial feed additive for the pigs, which is ecologically environmentally-friendly, the content of beneficial viable count in each gram of main ingredients is greater than 1.6 billion, and 2%-8% of the additive can be added in the feed for replacing an antibiotic medicament added in the pig feed, thereby solving the serious environmental pollution problem caused by manioc waste in a cassava starch factory; a product contains a large quantity of beneficial bacteria, thereby creating micro-ecological environments which are conductive to growth of the pigs in pig bodies, maintaining the normal physiological function of intestines of the pigs, promoting the digestion and absorption of nutrient substances in the intestines of the pigs and improving the utilization rate of the feed by 5%-10%; and simultaneously, metabolites of the beneficial bacteria can reduce the pH value in the intestines of the pigs, thereby effectively inhibiting the growth of pathogenic bacteria.

The invention provides chenopodium quinoa fermented milk and a preparation method thereof. The preparation method comprises the following steps: germinating chenopodium quinoa to prepare chenopodium quinoa sprouts; preparing chenopodium quinoa sprout powder from the chenopodium quinoa sprouts by shelling, washing, freezing and drying in vacuum and grinding; mixing the chenopodium quinoa sprout powder with pure water, and grinding to prepare chenopodium quinoa sprout milk; and gelatinizing, sterilizing and cooling the chenopodium quinoa sprout milk, inoculating lactobacillus plantarum and lactobacillus casei, and fermenting to obtain the chenopodium quinoa fermented milk. The chenopodium quinoa fermented milk has the protein content of more than or equal to 1.5 percent, acidity of 85-90 Thorner degrees, viable count of 9-9.59logCFU/mL and general flavone content of more than or equal to 25mg/100mL. The chenopodium quinoa fermented milk has milky yellow color, rich nutrition and unique flavor, completely accords with the requirements of people for nature, health and low sugar content, and has wide market prospects.

The invention relates to a selenium-rich chitosan nutritional microbial fertilizer. The selenium-rich chitosan nutritional microbial fertilizer is prepared from the following raw materials: sodium selenite, chitosan hydrolysate, compound trace elements, monopotassium phosphate, urea, peptone, yeast powder, NaCl, NaOH, NaNO3, NaCl, NH4Cl, K2HPO4, a beef extract, glucose, starch, calcium carbonate, amino acid, potassium nitrate, ferrous sulfate, magnesium sulfate, trisodium citrate, biochemical enzymes and a mixed bacterium liquid. Due to addition of the chitosan, the viable count of a microbial agent can be kept; the efficacy of the chitosan for keeping the viable count is put into an effective play; due to addition of the chitosan, the amount of bacteria of the microbial agent can reach 20,000,000,000/mL; due to addition of the chitosan to a bacterial manure, the yield of commercial crops can also be increased by 10%-15%; and the nutritional microbial fertilizer has the efficacies of keeping moisture and slowly releasing fertilizer efficiency.

The invention discloses an organic fertilizer using chicken manure and mushroom dreg as main raw materials and a preparation technology. The organic fertilizer comprises the following components in percentage by mass: 65 to 75 percent of chicken manure, 20 to 30 percent of mushroom dreg, 1 to 2 percent of biopreparate, and 3 to 5 percent of deodorant. The biopreparate consists of biological fulvic acid and enzyme microorganism, and the mixing ratio of the biological fulvic acid to the enzyme microorganism is 3-4:1-1.5. The deodorant is calcium superphosphate. According to the bioorganic fertilizer, the chicken manure and the mushroom dreg produced in the production process of nearby enterprises are fully utilized, so that pollution to the surrounding environment is reduced. According to the bioorganic fertilizer, environmental pollutants are converted into fertilizer substances required by agricultural production, so that waste is changed into valuable. The viable count of the bioorganic fertilizer is extremely high, so the hardened soil can be effectively improved and repaired; and the organic fertilizer can play a role in resisting diseases and stress. The production technology is simple in flow, easy to operate, short in production period (composting can be finished in 15 to 20 days), low in environmental pollution and low in cost; and the additional value of the organic fertilizer is high.

In the invention, a bacillus psychrosaccharolyticus obtained by screening is used for preparing an efficient complex microorganism seaweed fertilizer. The bacillus strain in the invention is named as bacillus psychrosaccharolyticus and preserved in the Common Microorganism Center under China Microorganism Strain Preservation and Management Committee with a preservation number of CGMCC No.4519. The bacillus psychrosaccharolyticus not only has the capacity of carrying out forced degradation on cellulose and starch but also has a wide antimicrobial spectrum; the bacillus psychrosaccharolyticus strain can be compounded with the traditional common strain for bacterial manure; then mixed zymocyte liquids, plant growth factors, immunoregulation factors, active extractives from seaweed, amino acids, organic matters and trace elements are prepared into an efficient complex mircoorganism seaweed fertilizer with an active viable count of 0.2 billion/g to 1 billion/g and an organic matter content of 20-40% according to a certain proportion. The fertilizer has remarkable capacity in combating plant diseases and insect pests and promoting growth. With the fertilizer, the yield of crops can be increased by more than 30%.

The invention discloses bacillus megatherium, a method for preparing a microbial inoculum through solid fermentation of the bacillus megatherium and an application of the microbial inoculum, belonging to the technical field of microbial preparations. The bacillus megatherium JSSW-JD-2F5 has been preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC NO.8045. By adopting solid fermentation, a preparation method of a bacillus megatherium JSSW-JD-2F5 solid fermentation microbial inoculum and the application of the solid fermentation microbial inoculum are disclosed, wherein the solid fermentation microbial inoculum is used as an additive of a livestock, poultry and fishery cultivation feed and is added into the feed according to a mass percentage of 0.01-0.05%. The product of the microbial inoculum contains high-concentration viable bacteria, active enzyme and physiological active matters, and the concentration of the viable bacteria reaches more than 1.0*10<10>CFU/g and can meet requirements of a feeding microbial additive on the viable count; the application of the inoculum not only can improve the breeding environment, but also has an important significance for increasing the feed utilization ratio and promoting the growth of animals, so that the inoculum is widely applied to livestock, poultry and fishery cultivation.

The invention discloses a lactic acid bacteria semi-solid fermentation product. The product is prepared by the following processes: inoculating lactic acid bacteria in raw materials and fermenting at a temperature of 24-44 DEG C for 24-40h to obtain the product. The raw materials comprise 4.0-5.0g of wheat bran, 1.2-1.8g of soybean meal, 0.8-1.2g of glucose or brown sugar, and water, wherein water is added until the total amount of the raw materials is up to 100mL. The inoculation amount of the lactic acid bacteria is 1.0-2.0*10<7>cfu/100mL, wherein the lactic acid bacteria is composed of Lactobacillus plantarum and Pediococcus pentosaceus with a strain viable count ratio of (3:10)-(10:3). The lactic acid bacteria semi-solid fermentation product for increasing growth performance of animals provided by the invention is the first fluid fermentation feed independently developed in China according to the national conditions for culture, inherits the advantages of the traditional solid fermentation feeds, and at the same time has the advantages of being more convenient, fresher, more significant in feeding effects, lower in cost and the like.

The invention discloses a bio-enzyme and microorganism containing compound feed additive and a preparation process thereof, which solve the problems of high production cost and less viable count of beneficial microorganisms in the prior art. The bio-enzyme and microorganism containing compound feed additive is characterized in that the additive is prepared by compounding a viable organism preparation (prepared by carrying out liquid fermentation and low-temperature drying on bacillus subtilis and brewer's yeast) with an enzyme preparation (prepared by carrying out solid fermentation and drying on trichoderma reesei and Aspergillus niger); and the viable count of beneficial microorganisms in each gram of the product is greater than 8*10<9> cfu/g, the cellulase activity (refer to the quantity of cellulose degraded by each gram of the product per minute) is greater than 500 mg, the alpha-amylase activity (refer to the quantity of starch hydrolyzed by each gram of the product per minute) is greater than 3000 mg, and the protease activity (refer to the mount of an amino acid generated by caseins hydrolyzed by each gram of the product per minute) is greater than 400 mg. The bio-enzyme and microorganism containing compound feed additive disclosed by the invention has the advantages that: the feed utilization rate of livestock can be improved in a short term, the intestinal digestive flora of livestock is improved and balanced, the breeding cost is reduced, and the feed conversion rate and the animal production performance are improved; and meanwhile, the additive is simple in preparation process, low in production cost, and suitable for industrialized production.

The invention relates to a Bacillus coagulans strain which is collected by China Center for Type Culture Collection, and the collection number is CCTCC M 2015273. By adopting the liquid fermentation technique, the Bacillus coagulans viable count in the fermentation liquid is 1.52*10<10>cfu/mL or above, the spore number is 1.38*10<10>cfu/mL or above, and the spore rate is 90.8% or above. In the dry bacterium powder obtained by spray drying, the viable count is 8.0*10<11>cfu/g or above. The dry bacterium powder can be mixed with starch, glucose or any other filler to obtain a Bacillus coagulans microecological preparation. The Bacillus coagulans has the characteristics of high fermentation density and high bacterium content, is beneficial to lowering the product cost, is convenient for popularization in the produce market, and has active meanings for promoting healthy livestock/poultry cultivation.

The invention discloses yoghourt ice cream and a preparation method thereof. The preparation method comprises the following steps of: uniformly mixing a yoghourt base material with an ice cream base material, charging gas, and regulating an expansion rate to obtain yoghourt ice cream; filling at a temperature of 2 DEG C-6 DEG C, and storing at the temperature of 0 DEG C-5 DEG C to obtain the yoghourt ice cream; a preparation method of the ice cream base material comprises the following steps of: heating and dissolving a thickener in water; adding milk powder, whey protein powder, cream and white granulated sugar in sequence; stirring for 10 minutes-15 minutes at stirring rotation speed of 700 rpm-1200 rpm, homogenizing, sterilizing and cooling; and stirring for 0.5 hour-3 hours at the stirring rotation speed of 100 rpm-200 rpm to obtain the ice cream base material. The yoghourt ice cream prepared by the method disclosed by the invention is smooth in mouthfeel, and mellow and natural in fragrance, contains probiotics and has viable count capable of reaching 107 cfu/g or higher.