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185 results about "Embedding Agent" patented technology

Electrochemical sensor capable of detecting trace mercury in water body, and preparation method and application thereof

The invention discloses an electrochemical sensor capable of detecting trace mercury in a water body. The electrochemical sensor comprises a gold electrode, wherein gold nanoclusters are deposited on the surface of the reaction end of the gold electrode; and sulfhydryl-modified mercury-specific oligonucleotide probes are self-assembled on the gold nanoclusters. The preparation method for the electrochemical sensor comprises the following steps of: first, preparing the gold electrode; then, electrodepositing the gold nanoclusters on the surface of the reaction end of the gold electrode; and finally, self-assembling the sulfhydryl-modified mercury-specific oligonucleotide probes on the end surfaces of the gold nanoclusters so as to finish the manufacturing of the sensor. By using the sensor provided by the invention, the trace mercury in the water body can be detected; and the specific operation comprises the following steps of: first, placing the reaction end of the sensor in a water sample for reacting; then, immersing the reaction end into an anion double-stranded deoxyribonucleic acid (DNA) signal embedded agent for complete treatment; later on, connecting the sensor into an electrolytic cell of a three-electrode system and measuring the change of a response peak current by using square wave voltammetry; and finally, judging whether the water sample contains mercury ions on the basis of the change. The electrochemical sensor has the advantages of simple and practical structure, convenience in manufacturing, high sensitivity, high selection specificity and the like.
Owner:HUNAN UNIV

Method for realizing synchronous nitrification and denitrification nitrogen removal by utilizing immobilized microorganism technology

The invention relates to the technical field of sewage treatment. The invention particularly provides a method for realizing synchronous nitrification and denitrification nitrogen removal by utilizingan immobilized microorganism technology. The adopted immobilized microorganism technology comprises the following steps: mixing a nitrifying bacterium agent and a denitrifying bacterium agent according to a certain ratio and adsorbing the mixture by using an adsorbent; embedding the adsorbed microbial inoculum by adopting an embedding agent; after embedding, adding dropwise a gel solution into across-linking agent to form gel beads with biological activity. The prepared gel beads sequentially form aerobic, anoxic and anaerobic environments from the surface to the inside which perform nitrification reaction, denitrification reaction and hydrolytic acidification respectively, nitrification liquid backflow is not needed, and synchronous nitrification and denitrification nitrogen removal canbe realized. Compared with a traditional biological denitrification process, the method has higher denitrification efficiency, and is simple in operation process, convenient to maintain and manage and easy to popularize.
Owner:CHAMBROAD CHEM IND RES INST CO LTD

Anaerobic ammoxidation sludge embedding immobilization method

The invention relates to an anaerobic ammoxidation sludge embedding immobilization method belonging to the field of sewage treatment, and particularly provides a method for performing embedding immobilization on anaerobic ammoxidation sludge by adding magnetic powder and powdered activated carbon. The method comprises the following steps: preparing an embedding agent, mixing anaerobic ammoxidation sludge with the embedding agent, preparing immobilized anaerobic ammoxidation balls, activating the immobilized anaerobic ammoxidation balls and the like. The preparation of the embedding agent comprises the following steps: weighing polyvinyl alcohol (PVA), sodium alginate (SA), powdered activated carbon and magnetic powder, and preparing into a solution, wherein on the basis of percentage in mass, the concentration of the PVA is 6-10wt%, the concentration of the SA is 2-3wt%, the concentration of the powdered activated carbon is 1-2wt%, and the concentration of the magnetic powder is 2-4wt%; and uniformly stirring, thus preparing the mixed embedding agent. The method is simple in preparation process, easy to operate and low in preparation cost; and the prepared embedding immobilization anaerobic ammoxidation balls are high in activity, favorable in stability and suitable for industrial production, and can achieve favorable economic and environmental benefits.
Owner:BEIJING UNIV OF TECH

Preparation method of flavone sustained-release algal inhibition preparation

The invention belongs to the field of water body pollution control, and provides a preparation method of a flavone sustained-release algal inhibition preparation. The flavone sustained-release algal inhibition preparation comprises 30% to 50% of 5,4'-dihydroxylflavone and 50% to 70% of embedding agent such as sodium alginate in percentage by weight, and has an entrapment rate of 50% to 70%. The preparation method of the flavone sustained-release algal inhibition preparation comprises the steps of: mixing a 5,4'-dihydroxylflavone dimethyl sulfoxide solution and a sodium alginate solution and mixing the obtained mixed solution and the mixed solution of chitosan and anhydrous calcium chloride; and then after performing the reaction for a certain time, filtering to obtain a precipitated flavone sustained-release algal inhibition preparation. According to the preparation method, the used materials are high in ecological safety; the prepared flavone sustained-release algal inhibition preparation can obviously inhibit the growth activity of bloom algae, particularly inhibits the growth activity of microcystis aeruginosa, and can control or manage outbreak of algal bloom in lakes. Compared with the prior art, the flavone sustained-release algal inhibition preparation has durable action time, and is particularly suitable for preventing and managing the algal bloom which frequently breaks out.
Owner:ZHEJIANG UNIV

Method for preparing brain tissue frozen section

The invention discloses a method for preparing a brain tissue frozen section, belonging to the fields of biology and medical experiments. The method comprises (1) a step of tissue extraction, immobilization and dehydration, namely a step of subjecting an animal brain tissue to immobilization and gradient dehydration; (2) a step of freezing pretreatment, namely a step of washing the brain tissue with a PBS solution, wiping the brain tissue, leveling the brain tissue, wrapping the brain tissue with tin foil paper, freezing the brain tissue in a rapid freezing zone of a freezing microtome, and then taking the tissue out for subsequent treatment; (3) a step of semi-embedding treatment, namely a step of subjecting the brain tissue to semi-embedded treatment, and allowing the tissue to stand for1 minute; (4) a step of frozen sectioning, namely a step of fixing a sample holder to a probe of the microtome, and performing continuous coronal frozen sectioning; and (5) a step of section collection, namely a step of collecting sections of desired positions into a six-pore plate containing a frozen preserving fluid, and storing the sections at -20 DEG C for long time. Semi-embedding treatmentis adopted to treat the brain tissue, and therefore, influences of an embedding agent on the brain tissue are avoided, and sections are complete and free of ice crystals, and have good immunofluorescent staining properties.
Owner:SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE

Resin embedding method for biological tissues marked by fluorescent protein and application of alkaline solution

The invention discloses a resin embedding method for biological tissues marked by fluorescent protein. The resin embedding method comprises the following steps: (1), fixing the biological tissues marked by the fluorescent protein in a chemical fixing manner to obtain the fixed biological tissues marked by the fluorescent protein; (2), replacing the fixed biological tissues marked by the fluorescent protein with an organic solvent, so that the biologic tissues are dewatered to obtain dewatered biological tissues marked by the fluorescent protein; (3), performing embedding agent permeation treatment on the dewatered biological tissues marked by the fluorescent protein to obtain embedding-agent monomer-filled biological tissues marked by the fluorescent protein; (4), enabling the embedding agent to generate a polymerization reaction to obtain a resin-embedding sample of the biological tissues marked by the fluorescent protein; (5), soaking the resin-embedding sample in an alkaline solution, and adjusting the pH value to be 8-12. The resin embedding method for the biological tissues marked by the fluorescent protein can be suitable for biologic tissue samples of various sizes, the fluorescence intensity is high, the imaging effect is good, and the samples have excellent cutting performance.
Owner:HUAZHONG UNIV OF SCI & TECH

Production technology of high-calcium tartary buckwheat flavone instant beverage

The invention discloses a production technology of a high-calcium tartary buckwheat flavone instant beverage. The production technology is a novel tartary buckwheat deep-processing method. The production technology comprises 1, extracting an optimal tartary buckwheat flavone water-extraction blended agent at a ratio of tartary buckwheat to water of 1: 8 at a temperature of 100 DEG C for 60min, 2, drying the extract at a temperature of 125 DEG C in an oven to obtain tartary buckwheat flavone powder having flavone content of 11.18%, wherein optimal spray drying conditions of the optimal tartary buckwheat flavone water-extraction blended agent comprise that the content of an embedding agent in a flavone extracting solution is 10%; a feeding concentration is 30%; an inlet temperature is 125 DEG C; and an outlet temperature is 80 DEG C, and 3, adding an appropriate amount of active biological calcium powder into the tartary buckwheat flavone powder to obtain high-calcium tartary buckwheat flavone powder, adding 0.35g of xanthan gum, 0.15g of beta-CD and 0.45g of CMCNa into each 6g of the high-calcium tartary buckwheat flavone powder to obtain the high-calcium tartary buckwheat flavone instant beverage in a solid state, wherein the optimal brewing state of the high-calcium tartary buckwheat flavone instant beverage is a suspension state. The brewing conditions comprise that 7g of the high-calcium tartary buckwheat flavone instant beverage is added into 250 to 300ml of water having a temperature of 80 to 90 DEG C so that an optimal taste is obtained. The high-calcium tartary buckwheat flavone instant beverage contains tartary buckwheat flavone having effects of reducing blood sugar, blood pressure and blood fat, and adopts high-calcium dried small shrimp powder rich in proteins so that calcium is reinforced.
Owner:HEBEI AGRICULTURAL UNIV. +1

Hexavalent-chromium reduction immobilization bacterial agent and preparing method

InactiveCN108018229AWill not destroy the structure and biological characteristicsEasy to spreadBacteriaWater contaminantsCalcium alginateRepeatability
The invention discloses an immobilized microbial hexavalent-chromium removing bacterial agent and a preparing method, and belongs to the technical field of chromium pollution site microbial remediation. The bacterial agent is prepared in the mode that calcium alginate serves as a carrier and is embedded with independently-separated wild bacillus. The preparing method includes the steps that an embedding agent and microbial bacterial suspension are evenly mixed according to the proportion of 1:1, the obtained thick mixed liquid is dropped into a CaCl2 solution and is dispersed into balls, coupling is carried out for a certain time, and the gel-state immobilization hexavalent-chromium removing bacterial agent can be obtained. The hexavalent-chromium reduction immobilization bacterial agent is compared with a free chromium reducing bacterium, the removing efficiency of hexavalent chromium can be remarkably improved, and the removing rate can be 100% at 96 h. According to the hexavalent-chromium reduction immobilization bacterial agent prepared with the method, the preparing method is simple and easy to operate, the reaction condition is mild, and the activity and the concentration ofmicrobial bacterial suspension can be efficiently kept; the formed bacterial agent is good in repeatability and stability, the microbial bacterial suspension is not prone to leakage, solid-liquid separation after reacting is facilitated, and the hexavalent-chromium reduction immobilization bacterial agent and the preparing method are suitable for industrial application.
Owner:CENT SOUTH UNIV

Device and technology for preparing engineering embedding fixing microorganism spheric bodies

The invention relates to a device and a technology for preparing engineering embedding fixing microorganism spheric bodies. Embedding medium solution in an embedding medium kettle is added into a mixing kettle, and a sorption agent in a sorption agent organism kettle and microorganism solution are added into the mixing kettle. After fully mixed, the two solutions enter a shunting head by an isolating valve and then are sprayed into a cross linker kettle. After solidifying and cross linking, spheric grains which are the microorganism embedding spheric bodies are obtained. The invention overcomes the defect that large-scale mass production can not carried out by the prior embedding fixing microorganism technology, divides liquid which continuously flows in a pipeline connected in front of the valve by the isolating valve so as to discontinuously provide feed liquid for the shunting head, and further shunts the feed liquid provided by the isolating valve by the shunting action of the shunting head so as to ensure that miscible liquid from the mixing kettle enters cross linker solution in a spraying mode to form precipitation of solid spheric grains. The invention realizes the mass production of microorganism embedding solid globes having good stability by the embedding fixing technology.
Owner:TIANJIN UNIV

Plant root freezing slice manufacturing method for synchronous radiation X-ray fluorescence microanalysis

The invention discloses a plant root freezing slice manufacturing method for synchronous radiation X-ray fluorescence microanalysis. According to the method, a sample is pretreated, an OTC (optical coherence tomography) freezing embedding agent is improved, the sample is soaked by the improved OTC freezing embedding agent, polyvinyl alcohol, polyethylene glycol and polyvinylpyrrolidone are mixed according to a certain proportion to form the improved OTC freezing embedding agent, the temperature of a slice knife edge is controlled, a user observes whether hardness and softness degrees of the sample and the improved OTC freezing embedding agent are consistent in trimming or not, and the success rate of a fresh plant root slice can reach 100% by prolonging freezing time and suitably improvingtemperature. According to the method, a fresh plant root tissue freezing slice with an integrated structure can be acquired, and a strong technical support is provided for development of synchronousradiation X-ray fluorescence microanalysis of fresh plant root tissues, research of absorption and accumulation micro-zonal distribution of heavy metals, nutrient elements and microelements in plantsand the influence of the heavy metals on physiological ecology of the plants.
Owner:INST OF QUALITY STANDARD & DETECTION TECH YUNNAN ACAD OF AGRI SCI
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