34 results about "Embedding Agent" patented technology

The invention discloses a coated titanium anode and an embedding preparation method thereof, and belongs to the technical field of coated titanium anodes, metal salt powder, an organic additive and inert filler are fully mixed to form embedding agent powder, the mass fraction of metal salt is 45-59%, the mass fraction of the organic additive is 1-5%, and the mass fraction of the inert filler is 40-50%; the sintering treatment is as follows: the pretreated titanium substrate is completely immersed in the embedding agent powder, the sintering temperature is 400-600 DEG C, the sintering time is 1-3 hours, and the heating rate is 5-10 DEG C / min. According to the method, low-temperature sintering is realized by precisely regulating and controlling the components and the proportion of the embedding medium and combining process parameters such as low-temperature sintering, and the damage to a titanium substrate is avoided; and meanwhile, the coating binding force of the titanium anode is improved, the service life of the titanium anode is prolonged, and technical innovation and industrial value are achieved.

The invention relates to an organ-like microsphere embedding slicing method. The method specifically comprises the following steps: 1) preparing hydrogel microspheres and dyeing the hydrogel microspheres with fuchsin; (2) dehydrating the dyed hydrogel microspheres and the organoid together, then putting the dehydrated hydrogel microspheres and the organoid into an embedding mold, adding an embedding agent for embedding, and obtaining an embedded sample after the embedding agent is solidified; and 3) slicing the embedded sample, and receiving the sliced sample by using a glass slide. According to the method, the hydrogel microspheres are firstly dyed, and the dyed hydrogel microspheres and the organoid are embedded together, so that the peripheral area of the organoid can be obviously red, and the magenta dyeing area on the section sample can be clearly observed even after the section sample is sliced. The hydrogel microspheres dyed by fuchsin can be used as positioning marks of organoid-containing microspheres, can provide obvious prompts in the early transparent dehydration process and the later embedding section process, and effectively help an operator to judge the section repairing degree and the section collecting degree.

The invention provides a pre-embedding agent which takes organic sulfinate as a framework and is used for pre-embedding lithium or sodium, a pre-embedded lithium positive electrode, a pre-embedded sodium positive electrode, a secondary battery and a method for pre-embedding lithium or sodium by using the pre-embedding agent. The pre-intercalation agent provided by the invention has the advantages of high specific capacity, good stability, no residue and good compatibility with a battery system, and can be well suitable for lithium or sodium ion secondary batteries. The lithium or sodium pre-embedding method is safe and convenient in pre-embedding process, simple in process, low in cost and capable of realizing large-scale production.

The invention belongs to the technical field of fish ecology, and particularly relates to a nude carp otolith embedding method based on a longitudinal section and a preparation method and application of an otolith grinding sheet. The epoxy resin and the curing agent are mixed according to a certain mass ratio and then placed on the vegetable parchment to embed the longitudinal section of the naked carp otolith, the vegetable parchment is resistant to high temperature and high in strength and is not prone to deformation caused by high temperature, and the cured embedding piece is easily separated from the vegetable parchment; the embedding agent is low in shrinkage rate after being cured, the otolith is not prone to fracture, the hardness requirement of subsequent grinding can be met, adhesion is high, degumming is avoided in the subsequent grinding process, and fragmentation of the naked carp otolith is effectively avoided. According to the method, the embedding piece polished for the first time is immediately immersed in water, oxidation of the polished surface of the otolith can be prevented, subsequent polishing is continued, and the otolith grinding piece with clear wheel lines is obtained. The method is simple in operation process, does not need to use a high-cost and low-efficiency embedding mold, reduces the preparation cost, and improves the preparation efficiency.

The embodiment of the invention relates to a method for freezing an ultrathin section and a novel embedding medium for freezing the ultrathin section. The novel embedding medium for freezing the ultrathin section is applied to the method for freezing the ultrathin section; the method for freezing the ultrathin slices comprises the following steps: putting a sample to be sliced into a fixture filled with the novel embedding medium, and putting the sample and the fixture into a low-temperature medium for freezing to obtain a quick-frozen and fixed sample; wherein the novel embedding agent is frozen into an amorphous solid state in a low-temperature medium and is used for supporting a frozen section; putting the quick-frozen and fixed sample into a freezing slicer at a set slicing temperature for freezing and slicing; at the slicing temperature, the novel embedding agent is kept in an amorphous solid state; the frozen and sliced sample is subjected to rewarming treatment, the novel embedding agent is completely volatilized through rewarming treatment, and a pure sliced sample is obtained after rewarming treatment.

An embedding device used in a Nisin preparation process relates to the technical field of Nisin embedding equipment, a filling mechanism, a feeding mechanism, a conveying mechanism and a stirring mechanism are installed in a cylinder, the discharging end of the filling mechanism and the discharging end of the feeding mechanism correspond to the feeding end of the stirring mechanism, the driving end of the filling mechanism is connected with the rotating end of the feeding mechanism, and the driving end of the conveying mechanism is connected with the driving end of the stirring mechanism. The discharging end of the stirring mechanism corresponds to the feeding end of the conveying mechanism; according to the device disclosed by the utility model, an embedding medium is melted through the feeding mechanism, the melted embedding medium is conveyed into the stirring mechanism, Nisin is uniformly scattered in the stirring mechanism through the filling mechanism, the liquid embedding medium and the Nisin are uniformly stirred through the stirring mechanism, so that the Nisin is wrapped by the embedding medium, and the mixed liquid is conveyed through the conveying mechanism; and in the conveying process, the mixed materials are easily cooled, so that the mixed materials are easily solidified together, and workers can conveniently collect the mixed materials.

The invention relates to a composition for treating trivalent arsenic pollution and a preparation method and application thereof. The composition for treating trivalent arsenic pollution comprises an active oxidation agent, an embedding agent and a function enhancer, the mass ratio of the active oxidation agent to the embedding medium to the function enhancer is (20-50): (50-100): (1-5); the active oxidizing agent comprises an activating agent and an oxidizing agent, and the activating agent is iron-containing industrial solid waste; the oxidant is sulfite; the embedding agent comprises acidic kaolin and silica sol; the function enhancer is at least one of citric acid, tartaric acid or oxalic acid. According to the invention, industrial solid waste steel slag and sodium hydrogen sulfite are adopted to construct an efficient oxidation agent system for trivalent arsenic; by means of solid-liquid permeation and gel integration and electrostatic and hydrogen-bond interaction, the oxidation efficiency of a medicament system is remarkably improved, and conversion from trivalent arsenic to low-toxicity pentavalent arsenic is accelerated.

The application discloses a plastic embedding agent BMA / IBOMA resin, monomers of the resin are composed of butyl methacrylate (BMA) and isobornyl methacrylate (IBOMA) in a mass ratio of 6:1 to 7:2. The application further discloses a preparation method of the plastic embedding agent, which comprises the steps of mixing the two resins according to the mass percentage, and then adding a crosslinking agent and an initiator. The application further discloses a plastic embedding method compatible with multi-color fluorescence and immunofluorescence labeling, which adopts the BMA / IBOMA resin to embed a biological tissue sample. The embedding method has low cost, and the BMA and IBOMA are used as hydrophobic resin monomers to effectively maintain the fluorescence signal of the sample. In addition, the IBOMA can improve the cutting performance of the sample after embedding, and meet the 1-micron precision cutting requirement of the fMOST system.

The invention discloses a pediococcus pentosaceus microcapsule and a preparation method thereof. The composite embedding agent is prepared by an endogenous emulsification method. According to the microcapsule prepared by the invention, the storage stability, the heat resistance and the antibacterial activity of the pentose tablet ball STS-6 are remarkably improved. The mannan oligosaccharide and the sodium alginate are combined for use, the pullulan and the poly gamma-glutamic acid are combined for use, and the schizophyllum commune polysaccharide and the rice bran protein are combined for use for synergistic interaction.

The present invention provides an embedding container capable of providing an additional approach to methods for embedding cells and tissues. Provided is an embedding container having a placement area where an object to be embedded by an embedding agent can be placed, the embedding container comprising: a sidewall disposed so as to surround the placement area; and a guide part disposed between the placement area and the sidewall to guide the object to the placement area.

The application provides an embedding agent and a freezing embedding method for plant spatial transcriptomics and spatial metabolomics analysis, and belongs to the technical field of biotechnology. The embedding agent is composed of 2.5% hydroxypropyl methylcellulose and 7.5% polyvinylpyrrolidone in mass concentration, and the embedding agent is applied to plant spatial transcriptomics research for the first time, and fills the technical gap in the field. Meanwhile, the application realizes the combined application of spatial transcriptomics and spatial metabolomics in the same plant individual for the first time, and provides a systematic technical solution for plant spatial multi-omics research. Through the application, the spatiotemporal dynamic change of gene expression in the development process of plants can be accurately analyzed, and the metabolic pathway and key enzyme gene of natural products in medicinal plants are provided, which provides important technical support for the functional research and industrial development of medicinal plants.

A method for preventing the retinal pigment from being removed from the network, comprising the steps of pre-fixing liquid fixation, solidifying agent wrapping shaping, fixing liquid fixation, embedding agent embedding, melanin decomposition liquid decomposing melanin, pre-decoloring liquid pre-decoloring, decoloring liquid decoloring and rinsing liquid elution. A method for removing melanin is provided to effectively avoid the retinal RPE layer cell from being removed from the network, protein denaturation and nucleic acid degradation. The method mainly adopts the following steps: pre-fixing liquid to maintain the original state of tissue cells, solidifying agent to maintain the integrity of tissue structure, melanin decomposition liquid composed of various extracts to decompose melanin particles, pre-decoloring liquid and decoloring liquid to separate melanin, and rinsing liquid to elute the decomposed melanin on the surface of the tissue, etc. Under the premise of ensuring the integrity of the retinal tissue structure, not damaging the surface protein, nucleic acid and cell structure of the tissue section, effectively decomposing the melanin particles in the tissue section, maintaining the biological activity of various organelles on the section surface and meeting the needs of subsequent various histochemical staining.

The application relates to the technical field of material detection, in particular to a powder material cross-section sample and a preparation method thereof. The preparation method of the powder material cross-section sample comprises the following steps: mixing a powder material with an embedding agent to prepare a slurry; placing the slurry on a substrate to form a slurry layer, and after vacuumizing the slurry layer to remove air bubbles in the slurry layer, solidifying to prepare an embedding body; and performing argon ion polishing on the embedding body to cut the powder material, thereby preparing the powder material cross-section sample. The preparation method of the powder material cross-section sample can improve polishing efficiency, the cross-section is smooth and free of scratches, and the internal structure of powder particles can be truly indicated.

The invention provides a tissue embedding agent and a hard tissue sheet preparation method, and the tissue embedding agent comprises the following components in parts by mass: 80-600 parts of a methacrylic acid monomer, 1-8 parts of a chain transfer agent and 3-15 parts of a photocatalyst. The preparation method of the hard tissue comprises the following steps: (1) fixing the hard tissue; (2) dehydrating the fixed hard tissue by using a dehydrating agent; (3) infiltrating the dehydrated hard tissue with a penetrating fluid, wherein the penetrating fluid is a mixed solution of a tissue embedding agent and a dehydrating agent; the tissue embedding medium is the tissue embedding medium disclosed by the invention; (4) immersing the infiltrated hard tissue into the tissue embedding agent, and carrying out ultrasonic treatment to obtain a mixture of the hard tissue and the tissue embedding agent; and (5) irradiating the mixture of the hard tissue and the tissue embedding agent by using visible light until the mixture is completely cured. According to the invention, the problems of insufficient mechanical property, thermal stability and biocompatibility of the polymer after the embedding medium is cured are solved.

There is provided an embedding container. The embedding container according to an embodiment of the present application accommodates a subject to be embedded and is used to be cut along with the subject, and a proximate plane to the subject at an arrangement location to arrange the subject thereon has a cross-section different from that of a plane adjacent to the proximate plane. In addition, there is also provided an embedding method comprising: arranging the subject in the embedding container; and embedding the subject and the embedding container as a whole. Moreover, there is also provided an embedding apparatus, comprising: a moving unit which moves the embedding container; and an introduction unit which introduces a processing solution and / or an embedding agent into the embedding container.

The invention relates to the technical field of biology, and particularly provides a plant tissue embedding method and slicing method adopting a polyvinyl alcohol colloid aqueous solution, and the method comprises the following steps: 1) enabling the polyvinyl alcohol colloid aqueous solution pre-cooled to 0-4 DEG C to be in contact with at least one surface of one or more plant tissues; 2) immersing the one or more plant tissues in a freezing embedding medium which is contained in an embedding box and is pre-cooled to 0-4 DEG C; and 3) keeping the embedding box at-80 DEG C or below for a period of time until the frozen embedding medium is completely solidified.

The application provides a sample preparation method for transmission electron microscopy of a sample to be positioned based on a novel embedding agent, and effectively improves the toughness of an embedding block, improves the quality of an ultrathin section of a sample, reduces the situation of section falling and section cracking of the ultrathin section, realizes the demand of semi-thin positioning section of the sample, guarantees the integrity of a target structure of the sample, effectively solves the problems of semi-thin positioning difficulty and non-ideal imaging effect of a sample such as a kidney tissue embedded by the novel embedding agent, and provides a solution for further application and popularization of the novel embedding agent 812R, and can be used for high-quality electron microscope sample preparation of human or mouse kidney tissue, so that ultramicrostructure observation and pathological diagnosis become possible.

The invention provides a method for rapidly preparing a transmission electron microscope test ultrathin slice coal sample, which comprises the following steps of: crushing lump coal, embedding by using a room temperature curing embedding agent, grinding and polishing after a polished section is cured and formed, observing and identifying macerals in the coal under an optical microscope, selecting and marking the position where the ultrathin slice is required, and determining the thickness of the ultrathin slice coal sample. And cutting the selected area, carrying out secondary embedding by using a room-temperature curing embedding agent, and finally, completing slicing by using an ultrathin slicing machine. Compared with a traditional resin embedding slicing method, the method for rapidly preparing the transmission electron microscope test ultrathin slice coal sample has the advantages that point selection, point taking and secondary embedding are directly carried out on the polished section, the complex chemical treatment process of coal sample leaching and inorganic mineral removal is directly skipped, and the sample preparation step is simplified; acid and alkali agents are not used, so that the safety risk and the consumable consumption are reduced; visual point selection is achieved, and the accuracy of sample preparation is improved.

The invention provides a container for embedding. An embedding container according to one embodiment of the present invention is used for accommodating an object to be embedded and is cut together with the object, and a surface near the object at a placement site where the object is placed has a cross-section different from a surface adjacent to the surface near the surface. Also provided is an embedding method including a placement step for placing an object in the embedding container and an embedding step for embedding the object together with the embedding container. Also provided is an embedding device comprising a moving unit that moves the embedding container and an introduction unit that introduces a treatment liquid and / or an embedding medium into the embedding container.

The utility model belongs to the technical field of pathological sectioning, and relates to a sectioning auxiliary table and one-stop sectioning equipment, the sectioning auxiliary table comprises an object placing table, and the object placing table is used for being placed on an upper table top of a freezing slicer; the object placing table is provided with an object placing surface, the object placing surface is divided into object placing areas, and the object placing areas are used for placing at least one of an embedding box, a slide, a blade and an embedding medium. According to the utility model, at least one of an embedding box, a slide, a blade and an embedding medium can be quickly and directly taken and placed from the storage area at any time before and after a slicing process, so that the embedding box, the slide, the blade and the embedding medium are prevented from being mixed with other articles, the embedding box does not need to be found from a disordered article pile, and the working efficiency and the use convenience of an operator are greatly improved. Besides, when the freezing slicer needs to be used, the slicing auxiliary table can be directly placed on the upper table top of the freezing slicer, when the freezing slicer does not need to be used, the slicing auxiliary table can be taken down from the freezing slicer, the table top space of the freezing slicer can be effectively utilized, and it is guaranteed that the space and the environment of a pathological department are used orderly and normatively.

The invention discloses an acidic moisture-proof solid tooth cleaning agent and a preparation method thereof, and belongs to the technical field of daily chemical industry. Comprising the following components: fumaric acid, magnesium carbonate, cellulose ether, polyvinylpyrrolidone, chitosan, a friction agent, a foaming agent, a sweetening agent, a flavoring agent, a preservative, a nutrition enhancer, a lubricant, an opacifying agent, an antioxidant and an embedding medium. The preparation method comprises the following steps: 1) embedding fumaric acid with a first embedding agent, and sieving; 2) embedding magnesium carbonate with a second embedding medium, and sieving; 3) embedding hydroxypropyl methyl cellulose with an embedding medium, and sieving; the invention relates to an acidic moistureproof solid tooth cleaning agent, which is prepared by the following steps: 1) preparing an acidic moistureproof solid tooth cleaning agent, 4) embedding hydroxyethyl cellulose with a fourth embedding agent and sieving, 5) embedding a sweetening agent with a fifth embedding agent and sieving, and 6) uniformly mixing the materials obtained in the steps 1)-4) with other raw materials to obtain the acidic moistureproof solid tooth cleaning agent. The acidic moistureproof solid tooth cleaning agent can remove dental calculus, has good moistureproof performance, can be prepared into tooth cleaning tablets, tooth cleaning powder and tooth cleaning particles, and is convenient to carry.

The application discloses a version management automatic embedding agent method, which comprises the following steps: adding a label to a YAML file, setting the label version to be consistent with the access agent version; updating a first extension configuration in a K8S cluster, the first extension configuration being used for intercepting the YAML file containing the label; when the interception occurs, the first extension configuration calls a modification service to modify the YAML file; when it is detected that the YAML file containing the label is submitted to the K8S cluster for deployment, a second extension configuration intercepts the YAML file containing the label, the second extension configuration calls a Webhook, the Webhook acquires the label, and the corresponding version of agent configuration information is inquired according to the label version; the YAML file is modified to automatically embed the version of the agent application, and the modified YAML file is returned to the K8S cluster. Through the mode of solidifying the agent version in the application YAML file, it is ensured that the versions of the embedded agents of the same YAML file in different environment deployment are consistent, and through the automatic embedding of the agent, the manual configuration work of the developer is greatly simplified.

The invention relates to an embedding method of amino acid as well as a product and application thereof, the method comprises the following steps: spraying a first embedding agent solution on the surface of amino acid to obtain a first embedded material; spraying a second embedding agent solution on the surface of the first embedding material to obtain a second embedding material; and spraying the third embedding agent solution on the surface of the second embedding material to obtain the product, the first embedding medium comprises any one or a combination of at least two of sodium alginate, maltodextrin or alpha-cyclodextrine; the second embedding medium comprises any one or a combination of at least two of calcium gluconate, resistant dextrin, starch sodium octenylsuccinate or phospholipid; the third embedding agent comprises any one or a combination of at least two of gellan gum, xanthan gum and sodium carboxymethyl cellulose. Through multi-stage embedding, the bitter taste of the amino acid is improved, the solubility of the amino acid is increased, the embedding rate is high, and the amino acid can be stably applied to food.

The present invention provides an embedding container capable of providing an additional approach to methods for embedding cells and tissues. This embedding container 1 comprises: a filter 11 which has a surface on which an object 100 to be embedded by an embedding agent can be placed, and through which a chemical solution in which the object 100 is immersed can permeate; and a base material 10 that supports the filter 11 and accommodates the object 100. A space 12 that can be filled with the embedding agent is formed on the rear-surface side of the filter 11.

The invention provides a cell freezing and embedding method for immunohistochemical staining, and belongs to the technical field of biology. The cell freezing and embedding method comprises the following steps: collecting cells; adding an embedding medium into the cells, and uniformly mixing the cells with the embedding medium; uniformly mixing the cells with an embedding medium, and freezing in liquid nitrogen; after the embedding medium is solidified, taking out the pre-embedded block; and carrying out secondary embedding on the pre-embedded block by using an embedding medium to obtain the cell frozen block for immunohistochemical staining. The cell freezing and embedding method disclosed by the invention is low in cell loss, low in cost and simple to operate; and the obtained frozen block slice is low in difficulty, is beneficial to long-term preservation and reuse, is better in quality and good in antigen preservation effect when being used for immunohistochemical staining, avoids false negative, and has a good application prospect.

The invention relates to an embedding agent, application of the embedding agent and miso powder containing the embedding agent. The embedding medium is prepared by the following steps: (1) taking rice as a raw material, crushing the rice, adding medium-temperature amylase into the crushed rice, and carrying out enzymolysis, so that the viscosity of the material subjected to enzymolysis is less than or equal to 100BU, and the sugar content is 20-28%; (2) filtering the material subjected to enzymolysis to obtain filtrate with the particle size of less than or equal to 125 microns and the viscosity of less than or equal to 85 BU; and (3) homogenizing the filtrate until the fineness is less than or equal to 100 microns to obtain a homogenized liquid, namely a dispersion liquid in which the embedding medium is dispersed. The embedding medium provided by the invention is derived from the low-allergy raw material rice, is not easy to allergy and has a wide application scene; the stability is good, the embedding rate is high, and the flavor and the function of the to-be-embedded material are basically not influenced; the compound has good water solubility, is high in clarity after being dissolved, and has good performance in liquid products or brewing products.

A colored cryoembedding agent, its preparation method, and a cryoembedding agent kit relate to the field of frozen section technology and address the technical issues of ice crystals easily forming within tissue cells, poor continuity and integrity, and the inability to provide color identification. Polyvinyl alcohol and pure water are mixed and heated to a boil, followed by the addition of potassium bicarbonate, eosin Y, methylene blue, and alcian blue 8GX. After stirring and dissolving, the mixture is cooled at room temperature, followed by the addition of polyethylene glycol 400 and sodium benzoate. The solution is heated in a water bath until clear, and pure water is added to compensate for evaporation losses. Finally, sucrose and dimethyl sulfoxide are added and mixed uniformly to obtain the cryoembedding agent. The kit contains at least two different colored cryoembedding agents. The present invention can be applied to tissue embedding in frozen sections, and is particularly suitable for pathological testing of breast cancer, thyroid cancer, and other cancers to distinguish tissue sites within the same patient.

The invention discloses a switchgrass sowing propagation method, and belongs to the technical field of switchgrass propagation, a composite embedding agent is prepared to embed switchgrass seeds, then low-temperature sand storage stratification treatment is carried out, fluridone is dynamically released through the composite embedding agent, low-temperature treatment is combined to promote switchgrass seed dormancy breaking, and the switchgrass seed dormancy breaking rate is increased. The composite embedding agent can continuously provide an appropriate humidity environment for the seeds after the stratification treatment is completed, so that the secondary dormancy of the seeds is inhibited, the germination rate of the seeds is improved, the sowing and propagation efficiency of switchgrass is improved, and the method has a good application prospect in the field of switchgrass propagation.

The present disclosure provides methods of detecting analytes such as RNA and / or DNA, extracting analytes such as RNA and / or DNA, improving library construction for nucleic acid sequencing, and reducing the level of embedding agent in analyte samples such as RNA and / or DNA samples that are extracted from embedded samples such as paraffin-embedded samples.

The invention relates to the technical field of biology, in particular to a composite embedding agent and application thereof in frozen sections. The composite embedding agent is prepared from PEG, PVA, glycerin and beta-cyclodextrin according to a specific ratio. Compared with a traditional paraffin-agarose embedding method and a traditional OCT embedding method, the completeness of the three-dimensional structure of the organoid can be more effectively maintained by using the composite embedding agent for embedding, the antigen can be better retained and protected, the antibody combination is more uniform, and the composite embedding agent is suitable for various staining modes, high in staining success rate and reliable in result. Meanwhile, the method is simple and easy to operate, reduces the risk of experimental errors, and has a good application prospect.