Manufacturing method of coconut blade freezing slice

Abstract

The invention relates to a manufacturing method of a coconut blade freezing slice. The manufacturing method comprises the following steps of cutting a coconut blade into a sample section, pasting a paper cuboid on a base, vertically placing the blade tissue sample on a sample support, fully wrapping the sample by using a freezing embedding agent, and placing on a sample platform for freezing slicing; and pasting the slices on a glass slide, adding a cover glass through roasting and dyeing, and observing and photographing the slices which are dry under a microscope. The manufacturing method provided by the invention has the advantages that a freezing slicing manner is adopted to rapidly obtain the freezing slice with an integrated structure, the period for manufacturing slices is effectively shortened, the material is not easy to deform, a microscopic observation effect of slices is good, the identification, evaluation and research on a coconut germplasm resource are rapidly developed, the structural observation and classification and identification of the coconut germplasm resource have the important meaning, and the foundation is laid for massively cultivating new coconut varieties in China.

Description

technical field [0001] The invention belongs to the technical field of microscopic tissue sectioning, and relates to a method for making frozen sections of plants, in particular to a method for making frozen sections of coconut leaves. Background technique [0002] Coconut (Cocos nucifera L.) is a typical tropical and subtropical woody oil crop and food and energy crop. It has the advantages of low investment, simple management, easy growth, long economic life, low investment risk, strong resistance to natural disasters, and rich nutrition. Wide range of uses and other advantages, deeply loved by the people. The whole body of coconut is a treasure. Coconut meat can be processed into copra, desiccated coconut, coconut bran, coconut milk and coconut oil. Coconut water can be processed into high-fiber coconut jelly. Coconut coat can be processed into high-grade mattresses, cushions, soilless cultivation media and Special organic fertilizer; coconut hard shell can be processed ...

AI Technical Summary

Problems solved by technology

The freehand sectioning method refers to the method of cutting the experimental materials into thin slices with a hand-held blade. Since the sample cannot be fixed, it is easy to produce uneven thickness when slicing, and it is difficult to obtain a complete section; although the paraffin sectioning method is the most commonly used method, due to The material needs to go through a series of steps such as fixation, dehydration, transparency and embedding before slicing. The process is cumbersome and the material is easily deformed. The production cycle is short, the observation effect is good, and the obtained material has a complete structure

[0005] At present, the research on the anatomical structure of coconut is mainly by making paraffin sections of leaves, and then observing its structural features under a microscope, but it takes 40 hours or more to make paraffin sections, and the efficiency is low. Research on structural microscopic observation has not yet been carried out

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