415 results about "Cocos nucifera" patented technology

The invention provides a method for sterile sowing and efficient propagation of cymbidium faberi. The method comprises the following steps: pretreatment of cymbidium faberi seeds, sterile germination of the cymbidium faberi seeds, protocorm proliferation, differentiation, seedling strengthening and acclimatization and transplantation. The pretreatment comprises the steps of refrigerating hybrid seeds of cymbidium orchid at 4 DEG C, performing secondary disinfection with sodium hypochlorite, performing dark culture for 10-30 days after sowing, and then performing light culture, and the germination culture medium is prepared by taking 1 / 4 MS as a basic culture medium and adding 100-200ml / L of coconut water, 0.5-1.0 mg / L of 6-benayl aminopurine, 0.1-0.5 mg / L of naphthylacetic acid, 0.5-2.0 g / L of peptone and 0.5-1.0 g / L of activated carbon. According to the method, the seed germination rate can be effectively increased, the 60d seed germination rate reaches up to 85%, the pollution rate is reduced to 5% or below, 50.0 g / L inositol, 2.0 mg / L glycine and 2.0 mg / L nicotinic acid are added into a differentiation culture formula, the differentiation rate can be effectively increased, the leaf bud differentiation rate reaches up to 90%, meanwhile, rooting can be induced, the growth time of cymbidium faberi from seeds to seedlings is greatly shortened, a large number of high-quality seedlings can be obtained within a short time, and the method is suitable for large-scale popularization and application. Technical data are accumulated for cymbidium resource protection and new variety cultivation.

The invention discloses a coconut strong promoter, a plant expression vector and application. The nucleotide sequence of the promoter is as shown in SEQ ID NO. 1. Compared with a known promoter, the promoter provided by the invention can drive high-efficiency expression of related genes, and is a strong promoter. The invention also relates to a plant expression vector containing the strong promoter and application of the plant expression vector in promoting expression of a target gene.

The invention relates to a palm crop protoplast gene editing method based on a CRISPR / Cas9 system and application of the palm crop protoplast gene editing method, and belongs to the technical field of biology. A carrier containing PDS gene editing targets and Cas9 protein genes of palm crops is successfully delivered to protoplasts of the palm crops such as coconuts and areca nuts through protoplast transformation for the first time, and the carrier contains sgRNA sequences designed according to PAM sequences recognized by Cas9 protein. The sgRNA and Cas9 compound formed after expression in palm crop protoplast cells can be edited at a PDS gene editing target. According to the method disclosed by the invention, an editing carrier based on the CRISPR / Cas system can be delivered into the palm crop protoplast, the method is used for gene editing technology research of palm crops such as coconuts, betel nuts, oil palms, date palms and the like, and the feasibility of the method for editing the palm crop protoplast genome target gene based on the CRISPR / Cas 9 system is verified; and a rapid and effective method is provided for subsequently establishing an efficient palm crop protoplast gene editing technical system.

The invention belongs to the technical field of emulsion materials, and particularly relates to metal-polyphenol network modified coconut protein composite particles as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing a coconut protein-pectin compound by taking coconut protein and pectin as raw materials, adding a tannic acid solution into the coconut protein-pectin compound, mixing, adding a Fe < 3 + > solution, and mixing to obtain metal-polyphenol network modified coconut protein composite particles; according to the preparation method, tannic acid and the coconut protein-pectin compound are subjected to non-covalent interaction, Fe < 3 + > and phenolic hydroxyl groups on the tannic acid are subjected to coordination reaction, a metal-polyphenol network is formed through cross-linking of Fe-O coordination bonds, and a compact modification layer is constructed on the surface of the coconut protein-pectin compound, so that on one hand, waste coconut protein resources are utilized; on the other hand, the problem that composite particles prepared from pectin and coconut protein are prone to depolymerization is solved, the structural stability is improved, and meanwhile iron and polyphenol are supplemented.

The invention discloses biocontrol marine source penicillium and application thereof, and relates to the technical field of microorganisms. The biocontrol marine source penicillium is named as Penicillium sp.DSF059 and is preserved in the China Center for Type Culture Collection, and the preservation number of the biocontrol marine source penicillium is CCTCC NO: M 20232310. The invention discloses biocontrol marine-source penicillium. The biocontrol marine-source penicillium has effects on rubber fusarium wilt pathogenic bacteria, coconut gray leaf spot pathogenic bacteria, wheat scab pathogenic bacteria, cucumber phytophthora root rot pathogenic bacteria, avocado phytophthora root rot pathogenic bacteria, rubber anthracnose pathogenic bacteria, mango anthracnose pathogenic bacteria, sisal zebra stripe pathogenic bacteria, rubber red root pathogenic bacteria, bacillus subtilis and bacillus subtilis. The invention provides a new direction for the prevention and treatment of the tropical crop pathogenic bacteria, and has a very good development and application prospect, and the tropical crop pathogenic bacteria such as the pathogenic bacteria of the rubber brown root disease, the coffee anthracnose pathogenic bacteria, the stylosanthes guianensis anthracnose pathogenic bacteria and the banana anthracnose pathogenic bacteria can be prevented and treated.

The utility model belongs to the technical field of coconuts, and particularly relates to a large-diameter coconut opening device and a coconut, the large-diameter coconut opening device comprises a positioning seat, the positioning seat comprises an annular bottom plate part attached to a coconut shell, a plurality of plug pins inserted on the coconut shell are distributed on the bottom surface of the annular bottom plate part, an annular cylinder part is arranged on the upper side of the annular bottom plate part, and the annular cylinder part is arranged on the lower side of the annular bottom plate part. A germ opening in the coconut shell is located in a middle hole of the annular cylinder part, a straw and a coconut opener are contained in the inner side of the annular cylinder part, a sealing film capable of being torn off is arranged on an upper opening of the annular cylinder part, and the germ opening in the coconut shell can be found more easily due to the fact that the germ opening in the coconut shell is located in the middle hole of the annular cylinder part. The position of the germ opening is the position of the coconut eye, the position is weak, a hole can be conveniently formed, a straw can be conveniently inserted into the hole, and the straw and the coconut opener are arranged in the annular cylinder part in a sealed mode, so that a coconut product bag provided with the large-diameter coconut opening device is better in integrity and convenient to circulate and sell in the market.

The embodiment of the present application relates to an SNP locus combination for constructing a coconut SNP fingerprint map and its application, belonging to the technical field of molecular biology. The embodiment of the present application aims to solve the technical problem that the previous fingerprints are usually screened for molecular markers based on SSR markers or other experimental means, resulting in poor repeatability. The SNP loci of the embodiment of the present application include 260 SNP loci located on chromosomes Chr01 to Chr16. The SNP locus combination provided by the present application can be used for constructing a coconut SNP fingerprint map and rapid identification of coconut varieties, and the present application provides brand-new identity information exclusive to different coconut varieties, which can effectively identify the authenticity of individuals and trace the origin of individuals, and has good application prospects in aspects such as coconut germplasm resource classification and identification, and molecular-assisted breeding.

The invention relates to the technical field of daily chemical products, in particular to a composition with a tightening effect and application of the composition in the daily chemical products. The composition is prepared from the following raw materials: a vine calli extracting solution, a caramel pedicel extracting solution, a coconut tomato extract, soy isoflavone, a bacillus fermentation product, an aloe barbadensis extract, a centella asiatica leaf extract, hamamelis virginiana water, a radix sophorae flavescentis extract, a fructus cnidii extract, a folium artemisiae argyi extract, a chamomile extract and a rose flower extract. And a scutellaria baicalensis root extract and a solubilizer. The composition disclosed by the invention adopts various natural plant extracts, the safety is high, various combinations are matched with one another, a synergistic tightening effect is achieved, and the added tenuikalii extracting solution and kacip flower pedicel maca extracting solution have remarkable tightening and beautifying effects.

The utility model provides a coconut water recycling and filtering device for coconut meat processing, which relates to the technical field of beverage processing equipment and comprises a supporting box, a rotating cylinder is arranged at the top of the supporting box, a placing cavity is arranged on the top surface of the rotating cylinder, a clamping plate is embedded in the placing cavity and matched with the placing cavity, and a movable filtering box is attached to the bottom surface of the supporting box. The shape of the movable filter box is the same as that of the supporting box, flexible filter plates are arranged in the movable filter box in an array mode, coconuts needing coconut water extraction can be rapidly placed through a rotating cylinder, the placed coconuts are rapidly limited in a clamping mode through clamping plates, when the coconut water is recycled, only the placed coconuts need to be punched, and the coconut water can be recycled. The coconut water can be quickly and independently recycled by rotating the rotating cylinder by 180 degrees, the coconut meat and the coconut water do not need to be recycled on the same equipment, impurities doped in the coconut water recycling process are greatly reduced during coconut water recycling, the recycled coconut water does not need to be complexly filtered, and the structure is simple.

The invention belongs to the technical field of artificial board additives and microcapsules, and discloses an environment-friendly multifunctional coconut-flavored microcapsule for artificial boards and a preparation method thereof.Natural high polymer materials such as chitosan, gelatin and Arabic gum serve as wall materials of the microcapsule, natural coconut essence and quaternary ammonium salt antibacterial and mildew-proof agents serve as core materials of the microcapsule, and the microcapsule is prepared by mixing the wall materials, the core materials and the coconut essence and the quaternary ammonium salt antibacterial and mildew-proof agents. The coating is prepared through a complex coacervation method or an interfacial polymerization method in combination with a secondary coating process. The essence microcapsule solves the problems of single function, poor fragrance durability, insufficient heat resistance and pressure resistance, poor wall material environmental protection property and the like of the essence microcapsule for the artificial board in the prior art; the microcapsule prepared by the invention is controllable in particle size, has good heat resistance and pressure resistance, and meets the requirements of an artificial board production process; when the microcapsule is added into the artificial board, the artificial board can be endowed with lasting coconut fragrance and excellent antibacterial and mildew-proof performance, and the wall material adopts a natural environment-friendly material, so that the product additional value and the market competitiveness of the artificial board are remarkably improved.

The invention discloses a kitchen and bath flue environment-friendly production material processing device and a manufacturing method thereof, and is applied to the technical field of filler processing. The kitchen and bath flue environment-friendly production material processing device comprises a crushing mechanism, and the crushing mechanism comprises a shell I. When the kitchen and bath flue environment-friendly production material processing device is used, an operator preferentially puts coconuts into a sorting mechanism; and the crushing of the coconut shells is realized through the sorting mechanism. Wherein coconut shell blocks which are not completely crushed are mixed in the crushed coconut cellosilk, and a linkage assembly drives a blowing assembly to sort the coconut cellosilk and the coconut shell blocks on a sorting assembly, so that the coconut shell blocks are inclined and concentrated towards one side through the sorting assembly; the coconut cellosilk is concentrated towards the other side of the sorting assembly under the action of the blowing assembly; through the arrangement, crushed coconut fibers can be conveniently separated from coconut shell blocks which are not completely crushed, the fatigue caused by manual picking in the prior art is distinguished, the inconvenience and complexity of manual picking are reduced, and the manual labor amount is reduced.

The utility model provides an automatic coconut juice extractor, which relates to the technical field of coconut juice extraction and comprises an extraction table and a control cabinet, a V-shaped placing groove is arranged at the center of the extraction table, a cutting blade is arranged at the top of the placing groove, a coconut juice recovery box is bolted at an outlet at the tail end of the extraction table, and a cutting blade is arranged at the top of the cutting blade. A drawing filter cabinet is embedded in an outlet in the tail end of the extraction table and an inlet of the coconut juice recycling box, a coconut shell recycling channel is formed in the extraction table, a turning plate is arranged in the coconut shell recycling channel, and coconuts can be placed on the extraction table through a placing groove in a unique shape; a plurality of groups of coconuts can be simultaneously subjected to cutting and shell breaking treatment in a manner of cutting from top to bottom by matching with a cutting blade, the coconuts do not need to be manually limited and fixed by personnel during cutting, and meanwhile, the plurality of groups of coconuts can be cut at one time to discharge and recycle coconut juice.

A plant propagation composition consisting of an aqueous willow extract with natural indole-3-butyric acid and salicylic acid, a moringa leaf extract, aloe vera gel and coconut water, wherein the pH of the composition is adjusted to 5.5-6.5 and is intended for cold storage to promote adventitious root formation in cuttings.

The invention discloses a method for improving the biomass and flavone content of a saussurea involucrata cell culture, which comprises the following steps of: culturing yellow-white saussurea involucrata callus on a culture medium at the temperature of 24 DEG C, illuminating for 16 hours / day, subculturing once every 15 days and subculturing for three times to obtain yellow-white or yellow-green callus which grows vigorously and is compact in structure; the preparation method of the culture medium comprises the following steps: weighing MS, cane sugar, naphthylacetic acid, 6-benzyladenine, coconut extract and agar in a container, adding distilled water, fully stirring, fixing the volume to 1 L, uniformly stirring, and adjusting the pH value to obtain a basic culture solution; carrying out high-pressure sterilization on the basic culture solution, pouring a flat plate, and cooling to obtain a culture medium; wherein the coconut extract is one or two of coconut water or coconut milk. By adding coconut water or coconut milk, the biomass of the saussurea involucrata callus can be remarkably increased in cooperation with the MS culture medium, and compared with an existing report, the increasing multiple is 2-3 times that of the existing report.

The invention provides an extraction method of coconut globulin, and relates to the technical field of coconut protein extraction. The method for extracting the coconut globulin comprises the following steps: removing albumin from defatted coconut powder by using distilled water, performing nitrogen pressure-boosting drying, mixing with a NaCl solution with relatively low concentration, performing high-voltage pulse electric field circulation treatment, stirring and extracting the NaCl solution, freeze-drying, redissolving, dialyzing and purifying to obtain the coconut globulin. According to the method, the defects in the prior art are overcome, and the extraction rate of the coconut globulin and the purity of the final product are improved while the good stability and emulsibility of the coconut globulin are ensured.

The invention discloses a method for embrittling fresh coconut pulp, and relates to the technical field of food processing, and the method comprises the following specific steps: firstly, selecting fresh or frozen coconut pulp without deterioration and peculiar smell, and dicing (unfreezing before freezing) for later use; carrying out segmented color protection by using VC solutions with different concentrations, washing and draining; then carrying out blanching treatment, fishing out, cooling and draining; immersing the cooled coconut meat blocks into a sugar-salt mixed solution for sugar infusion for seasoning and dehydration, and treating at normal pressure or under the assistance of high pressure or ultrasonic waves; and finally, performing freeze drying under specific conditions, performing secondary drying until the moisture reaches the standard, performing cooling to room temperature, and performing bagging so as to obtain a finished product. Through a complete process, efficient and high-quality embrittlement of coconut pulp, VC color protection for inhibiting discoloration, blanching sterilization, salt and sugar permeation for promoting flavor permeation, and synergetic drying for retaining nutrition and flavor are realized; meanwhile, all links are precisely coordinated, the product quality and the production stability are improved, and the high-quality fresh coconut crisp blocks are provided for consumers.

The utility model discloses a novel coconut juice blending device, and relates to the technical field of coconut juice blending. The novel coconut juice blending device comprises a table top, a coconut juice barrel and a blending barrel are fixedly installed on the upper surface of the table top, a telescopic rod is fixedly installed at the top of the coconut juice barrel, and a positioning ring is fixedly installed at the top of the telescopic rod. Then a telescopic rod is controlled to drive a positioning ring to conduct positioning operation on the coconut, then a hydraulic rod and a tapping machine are controlled to drill holes in the coconut, so that the tapping machine conducts opposite-penetrating tapping on the coconut, then coconut juice flows into a coconut juice barrel from the hole in the bottom of the coconut, a net plate conducts filtering on the coconut juice, and then the coconut juice is communicated to any end of a connecting pipe through a micro water pump; and then the coconut juice is fed into the blending barrel through a connecting pipe, and the blended coconut juice is discharged from a discharging pipe, so that subsequent operation is facilitated, blending operation is facilitated after coconuts are opened in the using process, and the using convenience of the device is improved.

The invention discloses a shaping substrate for plant seedling raising and planting, and relates to the technical field of planting substrates, the shaping substrate for plant seedling raising and planting comprises the following components by mass: coconut fiber; fine coco coir; peat soil; an organic fertilizer; bark; straw powder; vermiculite; perlite; saw dust; calcium magnesium phosphate fertilizer; pond sludge; leaf mold; rice chaff ash; hard particles; and a plant growth regulator. According to the substrate disclosed by the invention, the original loose substrate is subjected to standardized shaping, and standardized production, optimized structural design and efficient resource utilization are utilized, so that high efficiency, environmental protection and economization of plant seedling culture and planting are realized, and the production efficiency, the resource saving rate and the plant growth quality are remarkably improved; meanwhile, diseases are reduced, pesticide use is reduced, the operation environment is improved, and healthy development of roots is promoted.

The invention discloses a preparation method of brown coconut yoghurt and yoghurt. The preparation method comprises the following steps: adding lipase into coconut milk for enzymolysis; mixing milk with the coconut milk subjected to enzymolysis, and performing browning to obtain a base material; adding sugar, starch and citrus into the base material, and mixing to obtain a mixture; sterilizing the mixture; and fermenting the sterilized mixture to obtain the brown coconut yoghurt. Coconut milk after enzymolysis is mixed with milk, browning is carried out to a certain degree, fermentation is carried out, the taste of part of the coconut milk after fermentation is changed, after browning, the product is appropriate in fragrance, the scorch aroma is increased, the roasted coconut yogurt with the rich flavor is prepared, the yogurt is brighter in color and full in color, the bitter taste of the product is reduced, and the unpleasant fragrance of a plant-based product is improved; the taste is smooth, the texture is stable, the state is more uniform, the texture is more uniform, and no water is separated from a substrate.

The utility model relates to the technical field of conveyors, and discloses an anti-extrusion conveying intelligent conveyor in coconut juice production, which comprises a conveyor belt mounted on two supports, a conveying box is arranged at the bottom of the conveyor belt, two sides of the conveying box are respectively connected with the two supports, the bottom of the conveying box is connected with a heating device, and the heating device is connected with the conveyor belt. Supporting legs are fixedly connected to the four corners of the bottom of the conveying box, hot water is contained in the conveying box, and the water surface of the hot water is lower than the bottom surface of the conveying belt. According to the anti-extrusion conveying intelligent conveyor in coconut juice production, coconuts are placed in hot water in the conveying box, and the coconuts float in the hot water, so that collision and extrusion between the coconuts and collision and extrusion between the coconuts and the box body are reduced, then damage of coconut meat in the coconuts is reduced, loss of coconut juice is reduced, the coconuts are boiled by using the hot water, and the production efficiency is improved. Therefore, the coconut fiber on the outer side is separated from the coconut shell, and subsequent coconut fiber removal is facilitated.

The present invention relates to a composition for wound treatment or skin regeneration, comprising an extract from one or more selected from the group consisting of Trichosanthes kirilowii (T. kirilowii), Pueraria lobata (P. lobata), Liriope platyphylla (L. platyphylla), Poria cocos (P. cocos), Prunus mume (P. mume), Astragalus membranaceus (A. membranaceus), Panax ginseng (P. ginseng), and Glycyrrhiza uralensis (G. uralensis). The composition has low cytotoxicity and exhibits wound-healing effects, particularly therapeutic effects on extensive skin ulcers such as diabetic skin ulcers.

The utility model relates to the technical field of coconut processing, in particular to a black skin removing device for coconut meat. Comprising an anti-skid base plate, a mounting shell, a motor, a feeding shell, spikes, an air cylinder and the like. A mounting shell is fixedly connected to the top of the anti-skid chassis, a containing opening is formed in the front side of the mounting shell, a motor is mounted at the top of the inner side of the mounting shell, a feeding shell is fixedly connected to an output shaft of the motor, at least one spine is fixedly connected to the bottom of the feeding shell, and an air cylinder is fixedly connected to the right side in the mounting shell. The semi-circular feeding shell and the spikes are arranged, coconut meat can be preliminarily fixed, secondary extrusion and fixation can be conducted through the first reset spring in the peeling mechanism, the coconut meat cannot be thrown out in the process that the feeding shell drives the coconut meat to rotate, and the protection sleeve and the second reset spring are further arranged, so that the coconut meat is prevented from being thrown out. The coconut meat can be extruded and fixed for the third time, it can be ensured that the black skin of the coconut meat is normally removed through a scraper, and the removing efficiency of the black skin of the coconut meat is improved.

This invention discloses a method for the synthesis of furfural and ethanol from green coconut (Cocos nucifera L.) husk. The method involves chemical reaction for furfural production and a biological fermentation for ethanol synthesis. In the chemical step, the green coconut husk undergoes acid hydrolysis in a high-pressure reactor, yielding furfural. The residual waste from this step is then utilized as a substrate for fermentation by Saccharomyces cerevisiae, producing ethanol. The integrated approach efficiently converts the cellulosic and hemicellulosic components of the green coconut husk into two industrially valuable compounds, furfural and ethanol, from a single agricultural waste stream. The invention provides a cost-effective and environmentally sustainable method for resource utilization and waste reduction.

The invention discloses a preparation and processing method of coconut-based metagen coffee, and belongs to the field of coffee processing. Coconut water is fermented by lactobacillus plantarum HNU531, a drying aid beta-cyclodextrin is added, and freeze drying is performed to prepare the coconut-based metagen, and the coconut-based metagen has good solubility. By additionally adding the coconut-based metagen into the instant coffee, the contents of gallic acid, isomaltose, trehalose and the like which are beneficial to a human body in the coffee can be increased. The sour taste and bitter taste of the coffee can be reduced, and the quality of the coffee is improved.

The invention relates to an anti-browning method in a coconut microspore somatic embryo culture process, and belongs to the technical field of coconut tissue culture. The anti-browning method in the coconut microspore somatic embryo culture process comprises the following steps: S1, anther collection and pretreatment; s2, anther observation, disinfection and separation; s3, anther culture and embryo culture. According to the anti-browning method in the coconut microspore somatic embryo culture process, PVP is added into the culture medium to resist the browning phenomenon in the coconut microspore embryo induction process; when the explant is cut, a citric acid solution is dipped to reduce browning caused by physical damage; ascorbic acid (vitamin C) is also added into the culture medium to adjust the pH value and resist the browning phenomenon; in addition, low-concentration TDZ (thidiazuron) is used as an induction hormone, so that the embryo induction efficiency is improved.

The invention belongs to the field of biology, and particularly relates to an MNP marker site, a primer composition and a kit for coconut variety identification and application of the MNP marker site, the primer composition and the kit. According to the 240 MNP sites of the coconut and the corresponding primer composition, multiple PCR amplification can be performed, sequencing of amplification products is performed in combination with a next-generation sequencing platform, and the 240 MNP sites of the coconut and the corresponding primer composition have the advantages of being high in throughput, high in distinction degree, high in accuracy and the like in detection of coconut varieties. The 240 MNP marker loci, the primer composition and the kit provided by the invention can meet the requirements of coconut variety authenticity identification and coconut variety distinguishing, can be used for constructing a DNA fingerprint database of coconut varieties, can quickly and accurately determine a coconut variety to be detected by comparing the variety to be detected with coconut variety data in the database, and can be used for rapidly and accurately identifying the coconut variety to be detected. And the method also has a huge promotion effect on selection of cultivated varieties in the coconut cultivation process and breeding of coconut varieties.

The utility model provides a peeling-free coconut mouth opener, and particularly relates to the technical field of coconut mouth opening, the peeling-free coconut mouth opener comprises a fixed handle, a cleaning mechanism and an assembling mechanism, a lower pressing rod is integrally formed on the outer side surface of the fixed handle, and a through hole is formed in the inner side surface of the lower pressing rod. When a coconut is opened, a coconut shell enters a through hole, then the coconut shell in the through hole can be cleaned by using an inserting rod, and when the coconut opening device is not used, a connecting pipe is rotated, so that the connecting pipe is in butt joint with a fixed handle, and a first threaded hole is communicated with a second threaded hole; when the movable handle is not used, the movable handle can be screwed to enable the threaded column to be separated from the first threaded hole, so that the movable handle can be rotationally stored, and a cavity is formed in the movable handle, so that the movable handle can be rotated to be stored in the cavity; and some small articles can be stored.

The application discloses a kind of preparation and suspension culture of blackland callus method, belong to the field of industrial biotechnology.It includes the following steps: after sterilization, take flower stem, access pH value is 5-7 in induction medium, under the condition of temperature is 20-30 DEG C and no light, induction culture 15-21 days, namely obtain blackland callus, wherein, the induction medium is made of MS basic medium, sucrose 25-35g / L, agar 6-7g / L, 6-BA 1.0-2mg / L, NAA 0.5-1.5mg / L, 2, 4-D 0.3-1.0mg / L and coconut water 0.5-1.5g / L composition.Under aseptic conditions, clamp the growth state of good blackland embryogenic callus 30g, inoculation is carried out in 200mL / 500mL pH value is 6.0 in MS liquid medium, temperature 25 DEG C, 110 rpm full light oscillation culture, subculture period 7 days, when subculture, the mixture of cell and culture fluid is mixed with fresh culture fluid according to 1:1 volume ratio, after equal division, continue to culture, namely obtain blackland suspension cell, wherein, the liquid medium is made of MS basic medium, sucrose 30g / L, 6-BA 1.5mg / L, NAA 0.5mg / L, 2, 4-D 0.5mg / L and coconut water 1.5g / L composition.The callus out of the rate of 88.6% is realized, and the induction rate of embryogenic callus that can be suspended culture is 68.6%-91.8%, and good callus state is provided for large-scale culture blackland cell.

The application discloses a lithium acetate-polyethylene glycol mediated protoplast lipid nanoparticle delivery method and belongs to the technical field of biology. 2+ )concentration to improve the PEG formula, successfully delivers the lipid nanoparticle (LNP) to the protoplast of oil palm, coconut and other palm crops, the mRNA of the nanoparticle is wrapped with enhanced green fluorescent protein (eGFP), and the fluorescent protein can be directly expressed in the protoplast with the mRNA as a template without a promoter. After the LNP is delivered to the protoplast of the palm crops by the method, green fluorescent signals are detected by laser confocal microscopy. The method can be used for directly delivering target gene mRNA to the protoplast of the palm crops, and can also deliver CRISPR / Cas system protein and the like for gene editing technology research.