The invention discloses a method for extracting
melanin from Taihe silky
fowl bones. The extraction method comprises the following steps: 1)
drying and crushing bone tissues of Taihe silky
fowl to obtain
bone tissue powder; (2) adding the
bone tissue powder into absolute ethyl
alcohol, mixing, refluxing in a water bath, filtering while hot after refluxing, collecting filter residues, and
drying to obtain degreased
bone tissue powder; (3) mixing the degreased bone tissue powder with water, pre-cooking, cooling, adding compound
protease, carrying out heat-preservation stirring, carrying out
enzyme deactivation, filtering while hot, collecting filter residues, and
drying, so as to obtain a deproteinized bone
tissue sample; and 4) soaking the deproteinized bone
tissue sample in
lactic acid, carrying out high-temperature extraction, centrifuging, discarding the supernatant, adding
lactic acid into the precipitate, carrying out high-temperature extraction, centrifuging, discarding the supernatant, taking the residue, washing and drying to obtain the
melanin in the Taihe black-bone
chicken bone. According to the method, non-
melanin components in bone tissues can be effectively removed through three steps of absolute ethyl
alcohol reflux degreasing,
protease enzymolysis and
lactic acid acidolysis.