35 results about "Passiflora" patented technology

The invention provides a passion flower PeGRX13 gene. The nucleotide sequence of the passion flower PeGRX13 gene is shown as SEQ ID NO: 1. The passion flower PeGRX13 gene is cloned from passion flowers for the first time, the transcription level of the PeGRX13 gene in the passion flowers is increased under drought stress, it is indicated that the gene can improve the drought resistance and the like of plants, research shows that the gene can remarkably improve the viability of yeast under the drought stress, the oxidation resistance of the plants is improved, and the drought resistance of the plants is improved. Plant leaves are slender, and the length of plant petioles is increased. The invention provides a new candidate gene for research in the aspects of improving the stress resistance of yeast and plants, changing the forms of leaves and petioles of plants and the like.

The invention discloses a whitening and freckle-removing composition, a conditioner, a medicine and a cosmetic. The whitening and freckle-removing composition is prepared from emblic leafflower fruit, passion fruit and Chinese ladiestresses root and leaf A; the phyllanthus emblica comprises a phyllanthus emblica raw material and / or a phyllanthus emblica extract, and the passion fruit comprises a passion fruit raw material and / or a passion fruit extract. Through scientific compatibility of the Chinese ladiestresses root-ginseng A and the second active component comprising one or more of the emblic leafflower fruit, the passion fruit and the salvia officinalis, the Chinese ladiestresses root-ginseng A has a remarkable inhibition effect on tyrosinase activity, can inhibit expression and functions of rate-limiting enzyme TYR in the melanin synthesis process, has a remarkable whitening and freckle-removing active effect, and has a remarkable whitening and freckle-removing effect. The composition is safe and efficient, and can be applied to various conditioners, medicines and cosmetics.

The invention provides a passiflora edulis virus detection primer group based on RT-RAA and CRISPR / Cas12a and application of the passiflora edulis virus detection primer group. The primer group comprises an RT-RAA amplification primer pair and a CRISPR / Cas12a detection primer and is used for specifically detecting the passiflora edulis virus, and the invention further provides a kit containing the primers, LbCas12a protein and ssDNA reporter molecules. The detection method comprises three steps of sample grinding, RT-RAA amplification and CRISPR / Cas12a detection, and a result is judged by observing fluorescence. The field visual rapid detection method established by the invention has the characteristics of high specificity, high sensitivity, low cost and the like, and is suitable for large-scale screening of field passiflora edulis and other crops infected with the passiflora edulis virus and quarantine detection before seedling transportation. The method provides powerful technical support for healthy seedling detection and field screening of diseases.

The present invention discloses a preparation method of compound total-nutrient and healthy dried Passiflora edulis Sims, comprising: (1) selecting fruit; (2) cleaning, digging a fruit pulp, and removing a fruit peel; (3) pressing the fruit pulp; (4) crushing the flesh; (5) mixing the Passiflora edulis Sims juice, the Passiflora edulis Sims slurry, liquid sugar or powdered sugar or high fructose syrup, apple juice or grape juice or lemon juice, adding citric acid and stirring; (6) pouring the Passiflora edulis Sims composite slurry into a mold, drying, removing the mold, sprinkling fine powdered sugar, and packaging to obtain dried Passiflora edulis Sims. The dried Passiflora edulis Sims in the present invention has aromatic flavor, good taste, sweet and sour taste, comprehensive and balanced nutrition, long shelf life, convenience in eating and suitability for all kinds of people to eat.

The invention discloses a visual detection kit for passiflora edulis virus based on RT-RAA-CRISPR / Cas12a and application of the visual detection kit for the passiflora edulis virus based on the RT-RAA-CRISPR / Cas12a. The kit comprises an RAA primer pair, crRNA corresponding to the RAA primer pair, Cas12a protein and report DNA (deoxyribonucleic acid). The invention further discloses a visual detection method for the passiflora edulis virus based on the RT-RAA-CRISPR / Cas12a. Experiments prove that the detection method has the characteristics of simplicity, convenience, rapidness, accuracy, high sensitivity, strong specificity and visualization, can accurately distinguish the passiflora edulis virus from other viruses with the capability of infecting passiflora edulis, provides a more convenient and efficient technical means for field diagnosis and prediction and forecast work of EAPV in China, and can be used for detecting the EAPV in China. And technical support and guarantee are provided for sustainable and healthy development of the passionflower industry in China.

A system (100) for the cultivation of Passiflora edulis (variety Panama Gold) with optimized fruit yield and plant architecture, consisting of: a seedling establishment unit (102) configured to monitor seed germination under controlled conditions and to evaluate seedling vigor using standardized measurements; an architecture identification unit (104) configured to assess the primary scaffold structure and identify dominant lateral shoots emerging from axillary meristems; an apical dominance management unit (106) configured to perform strategic apical pruning of the primary scaffold at predetermined heights; a lateral shoot selection unit (108) configured to establish an optimal secondary structure by selectively pruning and training tertiary shoots; a shoot hierarchy refinement unit (110) configured to optimize quaternary shoot development; and a productive growth control unit (112) configured to redirect the energy allocation of the plant from vegetative growth to reproductive development.

The invention relates to the technical field of bioengineering and fermentation engineering, and particularly discloses a method for improving expression of recombinant human epidermal growth factors by pichia pastoris, which comprises the step of adding carboxymethylation modified passion fruit peel polysaccharide derivatives into a culture medium in a pichia pastoris fermentation culture process. According to the method for improving the expression of the recombinant human epidermal growth factor by the pichia pastoris, a proper amount of carboxymethylation-modified passion fruit peel polysaccharide derivative (C-WPEP) is added into a pichia pastoris expression system, so that the expression quantity, stability and activity of the rhEGF can be remarkably improved, the operation is simple and convenient, the cost is low, and the method is suitable for industrial large-scale production of the rhEGF.

The invention relates to the technical field of extracts, in particular to a passion fruit peel extract and an extraction process thereof. The passion fruit peel extract comprises a flavonoid extract and a pectin extract, an extraction process of the flavonoid extract comprises the following steps: extracting flavonoid substances in passion fruit peels by using an enzymolysis method, and obtaining peel residues; the extraction process of the pectin extract comprises the following steps: mixing fruit peel residues, water and a eutectic solvent, and extracting pectin in the residues at 140-150 DEG C and 2-3 MPa; according to the extraction process, an enzymolysis method is combined with high-temperature and high-pressure eutectic solvent extraction, a flavonoid extract and a pectin extract are obtained, efficient separation and high-purity extraction of flavones and pectin in passion fruit peels are achieved, and maximization of the value of raw materials is achieved.

The application provides a kit and a method for rapidly detecting Passiflora edulis virus of East Asia, which are simple, have high sensitivity, high specificity, short reaction time (only 30 min), can be operated, reacted and stored at normal temperature, and are very suitable for large-scale rapid detection in the field.

The invention relates to the technical field of extraction of effective substances of natural plants, and particularly discloses a preparation method of a passion flower supercritical CO2 extract and application of the passion flower supercritical CO2 extract in preparation of a preparation for regulating pig immunization. A compound enzyme is added to hydrolyze passion flower seed slurry, so that cell walls and cell membranes can be effectively decomposed, more oil-phase components can be released, and the effect of improving the immunity of pigs is achieved. Carbon dioxide and nitrogen are mixed in proportion, the formed mixed gas can better dissolve and carry target components in the supercritical extraction process, drying is carried out under the vacuum condition of 40 DEG C, residual moisture and volatile solvents in an extract can be effectively removed, and the purity of the extract is improved. According to the preparation method of the passion flower supercritical CO2 extract, through the steps of optimization pretreatment, enzymolysis, pH value adjustment, mixed gas extraction and vacuum drying, the extraction efficiency and the product quality are remarkably improved, grease components in passion flower seeds can be extracted, and other active components can also be extracted.

The invention relates to the technical field of plant culture, in particular to a culture medium and a culture method for culturing passiflora edulis tissue culture seedlings and a culture method of passiflora edulis seedlings. The induction culture medium is based on an MS culture medium and further comprises thidiazuron, 6-benayl aminopurine, indoleacetic acid, cane sugar and agar. The proliferation culture medium is based on an MS culture medium and further comprises the following components: 6-benayl aminopurine, indoleacetic acid, cane sugar and agar. The rooting culture medium is based on an MS culture medium and further comprises indolebutyric acid, indoleacetic acid, cane sugar and agar. The culture medium disclosed by the invention can be used for culturing passion flower tissue culture seedlings with a large rooting number and a high rooting rate, and a new way is provided for breeding of good passion flower varieties.

The invention provides a passion flower PeHSF1 gene. The nucleotide sequence of the passion flower PeHSF1 gene is shown as SEQ ID NO: 1. According to the invention, the passion flower PeHSF1 gene is cloned from passion flower for the first time, the research shows that the gene can obviously reduce the viability of yeast under drought stress and high-salt stress, and under drought stress and high-salt stress, the germination rate of a PeHSF1 transgenic plant is obviously reduced, and the survival rate of the yeast under drought stress and high-salt stress is obviously increased. It is shown that the PeHSF1 gene can obviously reduce the drought stress resistance and high salt stress resistance of the plant, and can inhibit plant growth, make the plant grow slowly and the like. The invention provides a new candidate gene for researching the stress resistance of yeast and plants, regulating the growth of plants and the like.

The invention belongs to the technical field of gene engineering, and particularly relates to a passionflower PenCBP gene for improving abiotic stress resistance of plants and application of the passionflower PenCBP gene. The nucleotide sequence of the provided PenCBP gene is shown as SEQ ID NO.1. The PenCBP gene is obtained from passionflower through cloning for the first time, and the PenCBP gene can be used for improving abiotic stress resistance of plants. By means of genetic transformation of arabidopsis thaliana, the weight of seeds can be increased, the number of fibrous roots can be increased, the leaf area can be increased, inflorescence stems can be thickened, fruit pods can be increased and thickened, and the drought resistance, high temperature resistance and cold resistance of arabidopsis thaliana are improved. The invention provides a new reference gene for research on improvement of abiotic stress resistance of plants, change of plant phenotypes and the like, and provides a technical scheme for cross-species genetic improvement.

The present invention pertains to the method of obtaining a raw material with anti-aging, wound healing, spot-reducing, dark circle-reducing, and anti-hair loss activities for use in cosmetic products. The invention comprises the steps of obtaining H. cordata cell suspensions, adding 0.05- 0.5 mM MeJa to the H. cordata cell suspension culture medium on the 12th- 18th day of the culture, incubating the culture with MeJA for 60- 80 hours, isolating extracellular vesicles (EVs) from the culture medium after incubation, isolating small RNAs from EVs, and encapsulating the EV-derived small RNAs in cationic liposomes. The invention covers the use of individual or combinations of plants, including Houttuynia cordata, Centella asiatica, Cydonia oblonga, Malva sylvestris, Persea americana, Rosa damascena, Helichrysum arenarium, Glycine max, Prunella vulgaris, Symphytum officinale, Malva sylvestris, Scutellaria baicalensis, Pelargonium graveolens, Filipendula ulmaria, Passiflora edulis, Marribium vulgare, Linum tauricum, Lycium barbarum, Papaver rhoeas, Paeonia peregrina, Leontopodium alpinum, Prunus dulcis, Inula helenium, and Psoralea corylifolia, as the plant in the invention.

To provide a novel composition that enhances the expression of Factor XIII A (Coagulation Factor XIII A).SOLUTION: Provided is an agent for enhancing the expression of Factor XIII A (Coagulation Factor XIII A), containing as an active ingredient one or more extracts selected from an extract of a plant belonging to the genus Passiflora in the family Passifloraceae, an extract of a plant belonging to the genus Cynara in the family Asteraceae, and an extract of a plant belonging to the genus Echinacea in the family Asteraceae.SELECTED DRAWING: None

The present application belongs to the technical field of alternative immunomodulators, and particularly relates to a preparation method of a Passiflora edulis immunomodulator for pigs and application thereof, which comprises the following steps: inoculating activated gold flower fungus into a liquid culture medium to prepare seed liquid; pretreating Passiflora edulis fruits, cutting or crushing the Passiflora edulis fruits to prepare a fermentation substrate, and adding bran and / or corn powder as an auxiliary carbon source; sterilizing the fermentation substrate; inoculating the seed liquid to perform solid or liquid fermentation; in the fermentation process, the gold flower fungus secretes an enzyme system to destroy the cell wall of the Passiflora edulis, release and convert polysaccharides and flavonoid active ingredients; and drying and crushing to obtain the Passiflora edulis immunomodulator for pigs. The immunomodulator can be added into pig feed to significantly improve the spleen index, macrophage phagocytic activity, lymphocyte proliferation capacity and serum IgG level, reduce the diarrhea rate, and promote growth performance. The present application combines the gold flower fungus and the Passiflora edulis to prepare the immunomodulator for pigs, which can be used to replace antibiotics and promote healthy pig breeding.

The invention relates to a passionflower explant anti-browning tissue culture medium and a tissue culture method thereof, and relates to the technical field of passionflower tissue culture. The invention provides an anti-browning tissue culture medium for a passionflower explant. The tissue culture medium comprises the following components: an MS culture medium, passionflower juice, 6-BA, IBA, sucrose and agar. According to the method, the culture medium is improved, the explant is subjected to low-temperature treatment, and the explant is subjected to secondary disinfection, so that the pollution rate and browning rate of the explant are reduced, the survival rate of the passionflower explant in the tissue culture process is increased, the defect of high browning death rate in the passionflower tissue culture process is overcome, and the tissue culture efficiency of the passionflower is improved.

The utility model discloses a passiflora edulis fertilizing and planting integrated device, which relates to the passiflora edulis planting field, and comprises a seedling planting device and a fertilizer box with a material conveying hose, the seedling planting device comprises a steel pipe and a plurality of soil breaking plates rotatably connected to the bottom of the steel pipe, and the steel pipe is rotatably connected with two handles; the steel pipe is slidably sleeved with a sleeve detachably connected with the conveying hose, the top of the sleeve is elastically connected with the steel pipe, and a discharging opening corresponding to the conveying hose is formed in the steel pipe. The handle is movably connected with the sleeve through an upper pull rod; the soil breaking plate is movably connected with the sleeve through a lower pull rod; a distance measuring rod is detachably mounted on one side of the bottom of the steel pipe; the device further comprises a knocking mechanism, the knocking mechanism comprises a metal ball fixed to the sleeve and used for knocking the steel pipe, and the problems that time and labor are wasted and planting efficiency is not high in an existing mode that fertilization is conducted on passion fruits and then field planting is conducted are solved.

The present invention relates to methods of preparing a composition comprising beta-caryophyllene, alpha-humulene, and linoleic acid. Moreover, the present invention refers to methods of preparing a composition comprising a blend of one or more oils from the genus Copaifera and one or more oils from the genus Passiflora. The present invention further relates to methods of preparing a cosmetic product comprising such composition.

The invention discloses an active substance in passionflower pericarp and a separation method and application thereof, relates to the technical field of compound separation, and comprises separation of three lignin compounds and confirmation and application of a new compound shown as a formula I. According to the invention, fresh passionflower pericarp is subjected to ethanol extraction, and a liquid chromatography method is utilized to obtain the active substance in the passionflower pericarp. According to the method, three lignan compounds are successfully separated by using acetonitrile and water as eluents, and the compound shown in the formula I is confirmed to be a new compound and has an inhibition effect on tyrosinase activity.

The application belongs to the technical field of genetic engineering, and particularly relates to a Passiflora edulis for improving non-biological stress resistance of plants PenCBP gene and application thereof, the gene nucleotide sequence is shown as SEQ ID NO. 1 PenCBP gene, and the gene is first cloned from the Passiflora edulis PenCBP The gene can increase seed weight, increase the number of fibrous roots, increase leaf area, thicken the peduncle stem, increase and thicken fruit pods, improve drought resistance, high-temperature resistance and cold resistance of Arabidopsis thaliana by genetic transformation. The application provides a new reference gene for improving non-biological stress resistance of plants, changing plant phenotypes and the like, and provides a technical scheme for cross-species genetic improvement.

The present invention provides a passion fruit PeLOX2 gene, the nucleotide sequence of which is shown in SEQ ID NO: 1. The present invention provides a passion fruit PeLOX2 gene cloned from passion fruit for the first time. The gene can respond to fruit ripening, reduce the size of the fruit, accelerate fruit ripening, improve drought resistance, and make the leaves of the plant slender. The present invention provides a new candidate gene for research on improving plant stress resistance, accelerating plant fruit ripening, and changing plant leaf and fruit size.

This patent relates to a novel phytotherapeutic compound for topical use, specially formulated for the treatment of burns. The innovation lies in the use of a formulation that combines passion fruit leaves (Passiflora sp.) and refined pork fat, providing a natural and effective approach to the management of skin burns. Passion fruit leaves are processed by infusion to extract flavonoids, known for their anti-inflammatory and antioxidant properties, which reduce inflammation and oxidative stress in damaged tissue, facilitating healing. Refined pork fat, chosen for its rich composition of essential fatty acids and its emollient properties, serves as the base for the ointment, helping to absorb the active ingredients and to form a protective barrier that minimises transepidermal water loss and protects against infections. The method of obtaining the compound involves frying the fat to extract lipids, followed by filtration for purification. Subsequently, passion fruit leaf extracts are combined with the purified fat and the mixture is evaporated until it reaches the appropriate consistency for an ointment. This composition not only accelerates the healing of burns, but also significantly alleviates the pain and discomfort associated with such injuries. The compound offers an effective and safe alternative to conventional burn treatments, standing out due to its simplicity, efficacy, and use of natural ingredients, making it particularly valuable in regions with limited access to medical resources.

The application discloses a whitening and freckle-removing composition, conditioner, medicine and cosmetic. The whitening and freckle-removing composition comprises a composition of Phyllanthus emblica, Passiflora edulis and discodermolide A; the Phyllanthus emblica comprises Phyllanthus emblica raw materials and / or Phyllanthus emblica extract, and the Passiflora edulis comprises Passiflora edulis raw materials and / or Passiflora edulis extract. The application has a significant inhibitory effect on tyrosinase activity by scientifically compounding discodermolide A and the second active component comprising one or more of Phyllanthus emblica, Passiflora edulis and Salvia, can inhibit the expression and function of the rate-limiting enzyme TYR in the melanin synthesis process, has a significant whitening and freckle-removing activity, is safe and efficient, and can be applied to various conditioners, medicines and cosmetics.

The application discloses application of dihydroquercetin in passion fruit virus elimination and application of Pedu CYP75B gene in disease-resistant breeding, and belongs to the technical field of tissue culture virus elimination. The application discloses application of dihydroquercetin in preparation of a passion fruit virus elimination preparation and application of Pedu CYP75B gene in cultivation of a disease-resistant passion fruit variety, wherein the gene is used for increasing synthesis of dihydroquercetin, and the Pedu CYP75B gene is selected from the nucleotide sequences shown in SEQ ID NO. 5-SEQ ID NO. 7. The application further discloses a virus elimination method for passion fruit, which comprises the step of inoculating micro stem tips of passion fruit into a culture medium added with dihydroquercetin and culturing. The application provides a new scheme for virus-free seedling production and disease-resistant breeding of passion fruit.

The invention discloses a passion fruit planting light-driving seedling cultivation device and method.The passion fruit planting light-driving seedling cultivation device comprises a bottom plate, the two sides of the top of the bottom plate are each provided with a fixing frame, the top of the bottom plate is provided with a containing groove, the containing groove is internally provided with a rotating mechanism matched with the two fixing frames, and supporting rods are fixed in the fixing frames; the supporting rods on the two sides are connected with a plurality of seedling raising plates through a plurality of adjusting mechanisms, each adjusting mechanism comprises two adjusting mechanisms, the two adjusting mechanisms are located on the supporting rods on the two sides respectively, and a plurality of seedling raising pots are arranged at the tops of the seedling raising plates. The gear ring locking device has the beneficial effects that the movable limiting mechanisms can drive the locking assemblies to limit and release limiting of the gear ring, meanwhile, along with continuous movement of the movable limiting mechanisms, the corresponding adjusting mechanisms can be driven to operate, and therefore the gear ring can be adjusted, and the gear ring can be adjusted. And then the corresponding seedling raising plates are driven to move vertically.

The invention particularly discloses a composition and application thereof in preparation of a product with an effect of improving the activity of lymphatic endothelial cells. The composition is prepared from a schisandra chinensis fruit extract and aescin, the weight ratio of the schisandra chinensis fruit extract to the aescin is (1-3): (0.1-0.3). Furthermore, the composition also comprises a passion fruit extract. The weight ratio of the schisandra chinensis fruit extract to the passion fruit extract to the aescin is (1-3): (1-3): (0.1-0.3). Researches show that the composition can improve the activity of lymphatic endothelial cells by enhancing the mitochondrial function and promoting cell autophagy. The composition disclosed by the invention has the effect of improving the activity of lymphatic endothelial cells; therefore, the compound has important application value when being used as an active ingredient for preparing medicines or cosmetics with the effect of improving the activity of lymphatic endothelial cells.

The present invention relates to the field of bioengineering technology, and in particular to a passion fruit stem rot resistance gene PeLSRD1 and a molecular marker thereof, wherein the cDNA nucleotide sequence of the passion fruit stem rot resistance gene PeLSRD1 is shown in SEQ ID NO. 1. The present invention identifies the passion fruit stem rot resistance gene PeLSRD1, and develops a molecular marker closely linked to the gene, which can be efficiently used in passion fruit stem rot resistance breeding.

The utility model discloses a passionflower vine fixing device and a climbing frame, and belongs to the field of agricultural planting auxiliary tools. The fixing device comprises a body, a pair of clamping arms and a pair of connecting arms. Elastic pre-tightening force which enables the clamping arms to be away from each other is applied to the clamping arms, so that the device has an unfolded state and a folded state. In the unfolded state, the clamping arms keep a large distance so as to contain the stand column. The folding state can be switched by driving the body to rotate relative to the clamping arm, at the moment, the clamping arm overcomes elasticity to fold and clamp the stand column, and the body is parallel to the stand column. The device is further provided with a state maintaining mechanism to maintain the clamping state, and the body is provided with a vine guiding piece. The device is simple and convenient to operate, can be quickly assembled and disassembled through'clamping-rotating ', can be repeatedly used and does not damage vines. The climbing frame comprises at least one stand column and at least one fixing device, and orderly guiding and efficient fixing of vines are achieved.

The present invention belongs to the field of agricultural microorganisms, and discloses an endophytic Streptomyces marinus BXG368 of passion fruit and its application in the prevention and control of passion fruit diseases. The preservation number of the Streptomyces marinus BXG368 is CCTCC NO: M 20242382. This strain is reported for the first time to be simultaneously used for the prevention and control of passion fruit virus disease, phytophthora blight, basal stem rot, gray mold and canker disease, and has the effects of killing aphids and thrips, and has no cross-resistance with existing bactericides and fungicides. Compared with the current use of chemically synthesized pesticides for prevention and control, since it comes from the natural environment, it has the advantages of high efficiency, low toxicity, non-pollution of the environment and difficulty in generating drug resistance, and has broad application prospects.