This invention provides a method for preparing a visual sensor based on mulberry anthocyanins and its application in
aquatic product monitoring. Using mulberry anthocyanins as an indicator, combined with
citric acid,
chitosan, and
polyvinyl alcohol, a novel freshness sensor is constructed on
filter paper via an impregnation method. The anthocyanins (MAs) exhibit a significant color change within the
pH range of 3–12, and their main components are various
cyanidin and pelargonidin glycosides. Testing showed that the sensor prepared by the
membrane method exhibited the best stability, remaining viable for over 15 days at 4°C, and demonstrating high sensitivity to volatile
ammonia. Specifically, the sensor with 1.25% MAs added showed a sensitivity of 11.79%–18.35% to 0.025%–0.1% NH3 within 30 minutes. When used for monitoring the freshness of
Litopenaeus vannamei, the sensor's
color difference value (ΔE) was highly correlated with storage time and TVB-N value, with the color gradually changing from purplish-red to grayish-blue, achieving visual and qualitative detection of
aquatic product freshness.