The invention relates to antibacterial plant perfume, in particular to the antibacterial plant perfume and a preparation method thereof. The antibacterial plant perfume is mainly prepared from raw materials as follows: natural aromatic plant oil, absolute ethyl alcohol and distilled water, wherein the natural aromatic plant oil comprises rose absolute oil, patchouli oil, lavender oil, ylang ylangoil, rosemary oil, bergamot oil, eucalyptus oil, pine needle oil, peppermint oil, orange flower oil, chamomile oil, clove oil, thyme oil, geranium oil, rose oil, citronella oil, juniper oil and chimonanthus salicifolius oil. The antibacterial plant perfume is prepared through steps of dissolving, mixing, ripening, freezing, aging, filtering and filling. The antibacterial plant perfume has antibacterial, sterilizing, fragrant and cooling effects and functions of relieving itching, removing miliaria, relieving bromidrosis and mephitis and refreshing oneself. The antibacterial plant perfume can sent forth fragrant, strong and lasting aroma and makes people comfortable, pleasure and enchanted when sprayed to body skin, hair, clothes and bedding. Besides, the antibacterial plant perfume has quietly elegant color and harmonious and natural aroma, and the aroma holding time can be as long as 3-5 days.