59 results about "Rosmarinus" patented technology

The present invention discloses an organic fertilizer for promoting plant growth and soil improvement and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer comprises the following raw materials: decomposed chicken manure, soybean meal, straw powder, sweet potato vine, humic acid, composite microbial agent, rosmarinic acid, and a stimulant. The composite microbial agent is composed of Actinomyces millefolius, Pseudomonas stutzeri, and Bacillus velezensis, and the stimulant is a glucosamine sulfate solution. Rosmarinic acid, glucosamine sulfate solution, and composite microbial agent are added to the organic fertilizer of the present invention. These three components are compounded with straw, chicken manure, etc. to form an organic fertilizer. The synergistic effect of multiple substances promotes the growth of cabbage and increases yield on the one hand; on the other hand, it significantly improves the physical and chemical properties of the soil.

The invention belongs to the field of traditional Chinese medicine analysis and quality control, and relates to a specific chromatogram construction method and a quality control method of a salvia plebeia sample. The specific chromatogram construction method comprises the following steps: 1) preparing a test solution, a reference solution and a reference medicinal material solution; (2) carrying out ultra-high performance liquid chromatography to obtain a corresponding chromatogram; and 3) taking the chromatograms of the reference substance and the reference medicinal material as a contrast, determining a characteristic peak from the chromatogram of the test solution, and constructing a characteristic spectrum. Meanwhile, on the basis of qualitative analysis of the characteristic chromatogram, quantitative analysis of rosmarinic acid is added, and the quality control method of the salvia plebeia sample is relatively comprehensively established. The method is simple to operate, and methodology verifies that the reproducibility and stability are good, the recovery rate is reliable, the detection cost is low, the detection efficiency is high, and an important guarantee is provided for standard production of salvia plebeia samples.

The present disclosure discloses a composition for hair growth comprising Larix europea wood extract, Camellia sinesis leaf extract, Pisum sativum sprout extract, Curcuma longa (Turmeric) callus conditioned media, Hamamelis virginiana (Witch Hazel), Rosmarinus officinalis Leaf Oil, Glycerin, Glycine, Zinc Chloride, Sodium Metabisulfite, Gluconolactone, Sodium Benzoate, Calcium Gluconate, Tetrasodium ethylenediaminetetraacetic acid (EDTA), Saccharide Isomerate, Hyaluronic Acid, Hydrolyzed Hyaluronic Acid, Propanediol, Biotinoyl Tripeptide- 1, Alpha Glucosyl Hesperidin, Caffeine, Hydrolyzed Pea Protein, Polyalkylene Glycol Mono Butyl Ether, Acrylates / C 10-30 Alkyl Acrylate Crosspolymer, Panthenol, Niacinamide, Arginine, PEG-40 Hydrogenated Castor Oil, Ethylhexylglycerin, Phenoxyethanol, and water is added to the composition until the desired volume is achieved. A method for preparing the composition for hair growth is also provided.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for improving systemic lupus erythematosus and a preparation method thereof. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 15-30 parts of dry rehmannia, 12-26 parts of calcined turtle shell, 13-25 parts of chicken tendon ginseng, 11-18 parts of red peony root, 10-20 parts of white flower snake tongue grass, 6-18 parts of small rosmarinus officinalis, 4-15 parts of artemisia annua, 5-10 parts of centella asiatica, 4-12 parts of cimicifuga foetida, 3-10 parts of coix seed, 2-9 parts of bergamot slice, and 3-7 parts of raw licorice. Test results show that the traditional Chinese medicine composition for improving systemic lupus erythematosus prepared by the application can inhibit the expression of IL-10 and IL-17 in serum, reduce anti-dsDNA antibodies in serum, significantly improve the clinical symptoms of systemic lupus erythematosus, and has a wide application prospect.

This invention relates to a whitening extract from geranium, its preparation method, and its application, belonging to the field of plant extract preparation and cosmetic technology. The extraction method includes: preparing a eutectic solvent by mixing choline chloride and citric acid in a molar ratio of 2:1 to 4:1, and adjusting the water content to 30-70%; mixing geranium raw material powder with the eutectic solvent at a material-liquid ratio of 1:5-15 (g / mL), and performing ultrasonic-assisted extraction; concentrating the extract under reduced pressure and then freeze-drying to obtain a geranium whitening extract with a total flavonoid content of 1-4 mg / g, a total polyphenol content of 3-6 mg / g, and anthocyanin content of 1-3 mg / g. This extract can be used to prepare cosmetics or topical skin preparations with whitening, brightening, antioxidant, or pigmentation-improving effects, as well as a tyrosinase activity inhibitor. This invention provides a green and efficient method for extracting the whitening active ingredients from geranium.

The present invention relates generally to methods and compositions useful for treating skin, and in particular to improve the appearance of droopy, loose, sagging, and / or flaccid skin and / or to improve facial contouring. In some aspects, disclosed are methods comprising topically applying to the skin an effective amount of a topical composition comprising escin, Ascophyllum nodosum extract, caffeine, Coleus barbatus extract, Hedychium coronarium root extract, Argania spinosa oil, Rosmarinus officianalis leaf extract, and / or Morus alba fruit extract.

The invention discloses a salvia miltiorrhiza endogenous oligopeptide composition and application thereof. The composition contains salvia miltiorrhiza endogenous oligopeptides miPEP167b-5p and miPEP171b-1, wherein the concentration ratio of the salvia miltiorrhiza endogenous oligopeptides miPEP167b-5p to the miPEP171b-1 is 0.8-1.2. The oligopeptide used in the composition has high species specificity, does not promote the growth of other plants in the field, and avoids the ecological risk of a traditional broad-spectrum growth regulator. Field experiment results show that after the composition is applied to treatment, the root system yield of salvia miltiorrhiza is increased by 56% compared with that of a control group, the number of roots is increased by 435% compared with that of the control group, the rosmarinic acid content is increased by 43% compared with that of the control group, the salvianolic acid B content is increased by 81%, the tanshinone I content is increased by 214%, the tanshinone IIA content is increased by 75%, the leap-type improvement of the salvia miltiorrhiza medicinal material yield and quality is synchronously achieved, and the application prospect is wide. And reliable technical support is provided for green, efficient and high-quality development of the salvia miltiorrhiza industry.

The invention relates to a rosemary borneol dehydrogenase SrBDH mutant and application of the rosemary borneol dehydrogenase SrBDH mutant in d-borneol, and belongs to the field of biological pharmacy and enzyme engineering. In particular to a borneol dehydrogenase SrBDH mutant derived from rosemary, a coding nucleotide of the borneol dehydrogenase SrBDH mutant, a recombinant plasmid and a recombinant genetically engineered bacterium containing the recombinant borneol dehydrogenase mutant, and application of the borneol dehydrogenase SrBDH mutant as a catalyst to preparation of d-borneol by asymmetric reduction of d-camphor. The catalytic activity of the obtained mutant is remarkably improved compared with that of a wild type, the feeding amount of the highest substrate dextrocamphor can reach 100 mM, the concentration of the product dextroborneol is gradually increased along with the prolonging of time, the yield of the dextroborneol can reach 85.0% after the reaction is conducted for 24 h, and the e.e. Value can reach 98.5%. The product d-borneol is an important natural active substance, has a high added value, and has a significant industrial application value.

The present disclosure discloses a composition for anti-graying hair comprising Aloe barbadensis Leaf Extract, Camellia sinensis leaf extract, Rosmarinus officinalis Leaf Oil, Hamamelis virginiana (Witch Hazel), Neopicrorhiza scrophulariiflora root extract, Tetrasodium ethylenediaminetetraacetic acid (EDTA), Acrylates / C 10-30 Alkyl Acrylate Crosspolymer, Panthenol, Polyalkylene Glycol Mono Butyl Ether, Ethylhexylglycerin, Phenoxyethanol, Sodium Benzoate, Potassium Sorbate, Propylene Glycol, Biotin, Hyaluronic Acid, Saccharide Isomerate, Alpha Glucosyl Hesperidin, Dimethyl Isosorbide, Mandarin peel paste, N-Acetyl L-Tyrosine, Pentylene Glycol, Gluconolactone, Propanediol, and water is added to the composition until a desired volume is achieved. The method for preparing the composition for anti-gray hair is also provided.

To provide a freshness retaining agent excellent in retaining the freshness of chlorophyll.SOLUTION: The freshness of a chlorophyll-containing material is retained by using a freshness retaining agent containing rosmarinic acid, its glycoside, its derivative or its pharmacologically acceptable salt as an active ingredient. Preferably, the freshness of the chlorophyll-containing material is retained by bringing a freshness retaining agent containing rosmarinic acid derived from a perilla seeds extract, its glycoside, its derivative or its pharmacologically acceptable salts as an active ingredient into contact with plants, phytoplankton, algae or their processed products.SELECTED DRAWING: Figure 1

The application discloses a rapid detection method of rosmarinic acid in perilla seed, and belongs to the technical field of chemical detection. The rosmarinic acid in perilla seed is maximally dissolved by adopting steel balls combined with specific extraction solution to grind the perilla seed. Meanwhile, the extraction rate of the rosmarinic acid is further improved by adding an acid solution. In addition, the chromatographic conditions of high performance liquid chromatography detection of the rosmarinic acid are optimized, so that the detection time is obviously shortened, and the accuracy of the detection result is high. The rapid detection method provided by the application is simple, the raw materials are cheap and easy to obtain, and is suitable for rapid extraction and detection of rosmarinic acid in a large amount of small sample of perilla seed. Therefore, the extraction and detection method of the rosmarinic acid in the perilla seed has a good application prospect.

The invention belongs to the technical field of external medicine for treating bromhidrosis, and particularly relates to external medicine for treating bromhidrosis and a preparation method thereof.The external medicine for treating bromhidrosis comprises active ingredients and auxiliary materials, the active ingredients comprise Chinese ephedra roots, the root of kudzu vine, pepper, perilla frutescens, calendula officinalis, pericarpium citri reticulatae, fortune eupatorium herb, rhizoma atractylodis, a radix sophorae flavescentis extract, a purslane herb extract, a ginger extract, zinc oxide and citric acid; the auxiliary materials comprise IPM, Tween 20, rosemary essential oil and Kathon. The preparation method comprises the following steps: firstly, extracting Chinese ephedra root, kudzuvine root, pepper, purple perilla, calendula, dried orange peel, fortune eupatorium herb and rhizoma atractylodis with water; dissolving the sophora flavescens extract, the portulaca oleracea extract, the ginger extract and the citric acid in the traditional Chinese medicine extracting solution, and stirring until the materials are completely dissolved; zinc oxide, IPM and rosemary essential oil are mixed into a Tween 20 solution for ultrasonic treatment, and finally the mixture and Kathon are added into the mixed extracting solution. The bromhidrosis removing agent has a good bromhidrosis removing effect, no toxic or side effect and stable performance. The effects of sterilization, deodorization, hidroschesis, skin moistening, perfuming and the like are achieved.

The invention belongs to the technical field of natural anti-corrosion materials, and provides a natural anti-corrosion composition containing cinnamon bark extract and a preparation method and application of the natural anti-corrosion composition. The natural preservative composition provided by the invention comprises a cinnamon bark extract and a synergistic compound, the synergistic compound comprises a mangnolia officinalis bark extract, a perilla leaf extract, a rhizoma atractylodis root extract, a folium artemisiae argyi extract, a wrinkled gianthyssop herb extract, a radix ophiopogonis root extract, a pomegranate seed extract, a pomelo extract, a thyme leaf extract, a calendula extract, a hyperforin perforatum extract, a melaleuca alternifolia leaf extract, a ginger root extract and an eucalyptus globulus leaf extract; the mass content of cinnamyl aldehyde in the cinnamon bark extract is less than or equal to 20wt%, and the antibacterial composition has the advantages of broad-spectrum bacteriostasis, high stability, difficulty in discoloration and precipitation, acid and alkali resistance, high temperature resistance, safety and small irritation. The antibacterial composition is prepared from at least one of a ginseng root extract, a rosemary leaf extract, a papaya fruit extract, a chamomile flower extract, a soybean seed extract and a wheat bran extract, and the mass content of cinnamyl aldehyde in the cinnamon bark extract is less than or equal to 20wt%.

The invention discloses a method for detecting the content of rosmarinic acid in anchusa coupling flower. The method comprises the following steps: detecting rosmarinic acid in a test solution by using high performance liquid chromatography; the test solution is an oxtongue flower extracting solution; in the high performance liquid chromatography, the detection wavelength is 328 nm; a mobile phase A is a formic acid solution with the volume percentage of 0.05%-0.15%, a mobile phase B is acetonitrile, and gradient elution is carried out. According to the method for detecting the rosmarinic acid in the anchusa flowers, the accuracy, reproducibility and reliability of quantitative analysis of the rosmarinic acid are ensured by optimizing chromatographic conditions and performing methodological verification. The detection method disclosed by the invention is efficient, strong in specificity, high in precision and good in linear relationship, and can provide a scientific basis for measuring the content of rosmarinic acid in a sample.

The application discloses a traditional Chinese medicine composition for auxiliary treatment of prostatic hypertrophy and a preparation method thereof. The traditional Chinese medicine composition is prepared from Uncaria, ethanol extract of Dehaasia bibracteata, Akebia quinata, Nelumbo nucifera, Crossandra infundibuliformis, Rosmarinus officinalis, Prunus armeniaca, Cinnamomum cassia and Salvia officinalis by processing. The traditional Chinese medicine composition has the effect of treating prostatic hypertrophy. The traditional Chinese medicine composition is mainly used for prostatic hypertrophy, and the symptoms include frequent urination, urgent urination and incomplete urination. The traditional Chinese medicine composition can reduce the prostate index of a patient with prostatic hypertrophy, regulate the sex hormone level, reduce the prostate phosphatase activity, and has a remarkable effect on treating the problems of frequent urination, urgent urination and incomplete urination caused by prostatic hypertrophy. Meanwhile, the composition is prepared from traditional Chinese medicines as raw materials, is safe to use, has high bioavailability, and has no any toxic and side effects.

A cosmetic sunscreen composition comprises avobenzone and one or both of Rosmarinus Officinalis (Rosmary) Leaf Extract and Haematococcus Pluvialis Extract to stabilize the avobenzone.

The invention discloses a salvia miltiorrhiza quality evaluation method based on HPLC-DAD and multivariate analysis, and relates to the technical field of medicine quality evaluation, and the salvia miltiorrhiza quality evaluation method comprises the following steps: extracting effective components from salvia miltiorrhiza medicinal material samples collected from different regions by adopting an extraction solvent, the effective components at least comprise salvianic acid A sodium, rosmarinic acid, salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA, HPLC-DAD is adopted for chromatographic analysis, and the extracted effective components are subjected to multivariate statistical analysis. According to the system, the quality difference of salvia miltiorrhiza from different producing areas is evaluated, and scientific basis and data support are provided for standardized planting, producing area screening and clinical reasonable application of salvia miltiorrhiza medicinal materials.

This invention discloses the SmWRKY13 gene and its application in increasing the salvianolic acid content in Salvia miltiorrhiza, belonging to the field of genetic engineering technology. By constructing a recombinant expression vector containing the SmWRKY13 gene and transforming it into hairy roots of Salvia miltiorrhiza, transgenic hairy root lines overexpressing SmWRKY13 were obtained. The salvianolic acid content of these transgenic hairy root lines was detected. The results showed that overexpression of SmWRKY13 significantly increased the content of salvianolic acid components such as rosmarinic acid and salvianolic acid B in the hairy roots of Salvia miltiorrhiza, with the total salvianolic acid content reaching up to 5.72 times that of the control group. This invention provides an effective strategy for increasing the salvianolic acid content in Salvia miltiorrhiza using genetic engineering methods, which is of great significance for meeting market demand and promoting the large-scale preparation of salvianolic acid.

The utility model discloses a kind of rosmarinus officinalis essential oil processing with squeezing device, including squeezing box, the side fixed mounting of squeezing box has driving device two, the output end transmission of driving device two is connected with rotating disc, the side of rotating disc and near edge place rotationally connected with rotating rod, one end of rotating rod is rotationally connected with sliding rack, the outer surface sliding connection of sliding rack has multiple guide blocks, the side of multiple guide blocks is fixedly connected with the side of squeezing box. The utility model is by opening driving device two, makes rotating disc rotate, sliding rack reciprocating sliding in guide block, sliding rack is cooperated with rotating gear, makes rotating roller reciprocating rotation at the bottom of filter screen one, in the process of rotation, the patting rubber on rotating roller is patting to the bottom of filter screen one, rosmarinus officinalis in filter hole is pat out, prevent long time use filter screen one, and the filtering efficiency of filter screen one is lower.

The present invention is directed to a composition including the following combination of ingredients: Valeriana officinalis extract, Passiflora incarnata extract, Ocimum sanctum extract, Ziziphus jujuba extract, Rosmarinus officinalis extract, and Nigella sativa extract. The compositions can be used in methods of improving or promoting sleep and managing and reducing stress.

Topical polyherbal oleogel composition, comprising, in weight percent of the finished product: (i) Boswellia serrata CO2 select extract 1.0–5.0%; (ii) Curcuma longa phytosome 0.5–2.5% with curcuminoids complexed to phospholipids together with turmerone-rich turmeric essential oil 0.2–0.8%; (iii) Zingiber officinale CO2 select extract 0.5–2.5%; (iv) Hypericum perforatum macerate oil 3.0–10.0%; (v) Capsaicinoids in an equivalent amount of 0.01–0.05%, obtained from Capsicum oleoresin, and Mentha piperita oil 0.3–1.0% together with Rosmarinus officinalis oil 0.2–0.6%; (vi) a carrier phase of sesame oil and medium-chain triglycerides in a ratio of 2:1 to 1:1, and castor oil 8–12% and squalane 1.0–3.0%; (vii) a structuring phase of sunflower lecithin 0.8–2.0% and beeswax 3.0–6.0%, providing a lamellar, shear-thinning oleogel; and (viii) mixed tocopherols 0.1–0.3% and a rosemary diterpene antioxidant extract 0.05–0.2%;wherein the composition is anhydrous with a water activity ≤ 0.55, and wherein the mass ratio of total boswellic acids to total curcuminoids is 0.8:1 to 1.6:1 and the mass ratio of menthol to total capsaicinoids is 10:1 to 30:1.;

The invention discloses an extraction reagent and an extraction method for functional components of natural plants. The method is applied to extraction of curcumin in turmeric, xanthophyll in marigold, linalool in lavender, rosmarinic acid in basil, ellagic acid in pomegranate, anthocyanin in lycium ruthenicum, isorhamnetin in sea-buckthorn, ginsenoside in ginseng and baicalin in scutellaria baicalensis. According to the method, the potential of DES in replacement of a traditional solvent can be visually displayed, and the method is suitable for courses of chemistry, environmental science or materials science and helps students to understand core concepts (such as recoverability and low-toxicity design) of green chemistry. Experiment cost is low, operation is safe, and the device is suitable for teaching laboratories.

A cosmetic sunscreen composition comprises avobenzone and one or both of Rosmarinus officinalis (Rosmary) Leaf Extract and Haematococcus pluvialis Extract to stabilize the avobenzone.

The invention discloses an extraction method of a salvia miltiorrhiza-hawthorn medicine pair, and belongs to the technical field of traditional Chinese medicine extraction. According to the extraction method disclosed by the invention, the total content of tanshinol, chlorogenic acid, procyanidine B2, epicatechin, hyperoside, isoquercitrin, alkannic acid, rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone and tanshinone IIA is taken as an extraction index, ethanol is taken as an extraction solvent, and the salvia miltiorrhiza-hawthorn medicine pair is subjected to reflux extraction. The process parameters in the extraction method are optimized by combining a single factor test with a Box-Behnken response surface method, and the salvia miltiorrhiza-hawthorn drug pair extract with the effective component content not lower than 8.0% is obtained. Based on a multi-index evaluation standard, a process with a better extraction effect is provided for development and application of the salvia miltiorrhiza-hawthorn medicine pair.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a construction method and application of a hairy root system of dracocephalum heterophyllum benth with high rosmarinic acid content. According to the method, dracocephalum heterophyllum benth aseptic seedling stem segments are used as explants, agrobacterium rhizogenes LBA9402 carrying a rosmarinic acid synthase (RAS) gene expression vector is used for infection, co-culture and induction, and then positive transgenic hairy roots integrated with the RAS gene are obtained through multi-stage screening and identification. The dracocephalum heterophyllum hairy root system established by the invention has the characteristics of genetic stability, rapid growth and strong rosmarinic acid synthesis capability, the rosmarinic acid content is obviously increased, and an efficient biotechnology platform is provided for realizing large-scale and sustainable production of rosmarinic acid.

The application relates to the technical field of pharmaceutical analysis, in particular to a method for determining the content of rosmarinic acid in a standard decoction of wind wheel herb and a method for constructing a characteristic spectrum. The high performance liquid chromatography method is used for determination, and the conditions of the high performance liquid chromatography method include that octadecylsilane bonded silica gel is used as a filler of a chromatographic column; methanol is used as a mobile phase A, and a 0.1% formic acid aqueous solution is used as a mobile phase B, and gradient elution is carried out. The method has strong specificity, good repeatability and durability, high stability and high accuracy; the constructed characteristic spectrum and detection method can quickly and comprehensively realize the purpose of combined control of multiple components in the standard decoction of wind wheel herb, realize quality control of the wind wheel herb from medicinal materials to preparations, and are favorable for guaranteeing the effectiveness of clinical medication.

Aspects of the present disclosure are directed to inhibiting viability of triple-negative breast cancer (TNBC) cells. According to an aspect of the present disclosure, a composition for use in inhibiting viability of TNBC cells contains 1,8-cineole as an active ingredient, and an excipient. In an embodiment, the 1,8-cineole is contained in one or more nutraceuticals. As a result, the composition may be administered to TNBC patients following fewer regulatory hurdles, owing to the minimal toxicity concerns associated with nutraceuticals. In another embodiment, the one or more nutraceuticals contain a first extract formed of one or more of Elettaria cardamomum and Salvia Rosmarinus, and the composition also contains a second extract formed of one or more of Piper nigrum and Piper longum. In another embodiment, the composition contains one or more other active ingredients selected from flavonoids, terpenoids, or alkaloids. In another embodiment, the excipient is a nutraceutically acceptable excipient.