The present invention provides a quantitative measurement method using competitive immunochromatography, which has not been previously reported. The quantitative measurement method comprises: (1) creating a
calibration curve based on the
signal intensity of a
test line according to the concentration of a
standard material by using multiple strips, and calculating the concentration of a target
analyte by using the
calibration curve; (2) when the concentration of the target
analyte increases to a significant level on the
calibration curve of the
standard material, a labeled
signal of a detection ligand on a
control line remains unchanged in a case where a detection ligand of a target
analyte-detection ligand conjugate binds to and saturates a capture ligand on the
control line, making it impossible to estimate the concentration beyond that level; however, by designing a
binding site for the capture ligand on the
control line to be the target analyte of the target analyte-detection ligand conjugate, a
high concentration of the target analyte breaks down the triple conjugate of detection ligand-target analyte-capture ligand, leading to dissociation of the labeled detection ligand and thereby weakening the
signal (
hook effect); and (3) comparing the
signal intensity patterns of the
test line and the control line according to the calibration curve of the
standard material with the
signal intensity pattern of a sample target analyte, and determining how far the target analyte is from the saturation point of the detection ligand. This method is characterized in that, in cases where the target analyte is present in an excessively large amount and exceeds the maximum value of a measurement range (
dynamic range) of the calibration curve, the calibration curve using multiple strips and the configurations (
test line and control line) of an immunochromatographic device are designed differently to distinguish such cases.