10 results about "Laser-induced fluorescence" patented technology

The invention provides a high-enthalpy flow field particle concentration measurement system and method based on two-photon laser-induced fluorescence. The system comprises a laser, a high-enthalpy flow field wind tunnel module, an imaging module, an image processing module, a reflector, a three-dimensional galvanometer, a three-axis displacement table and a collecting lens. The centers of the reflecting mirror, the three-dimensional galvanometer and the collecting lens are coaxial and located on the same light path plane, and the three-dimensional galvanometer and the collecting lens are both fixedly installed on the three-axis displacement table; the laser device generates a laser beam, the laser beam is irradiated to the three-dimensional galvanometer through the reflecting mirror, the direction of the laser beam is changed by deflecting the three-dimensional galvanometer and / or moving the three-axis displacement table, and then the laser beam with the changed direction is irradiated to particles to be measured in a flow field area to be measured through the collecting mirror to generate fluorescent light; the imaging module collects fluorescence generated by irradiation of the laser beams to obtain fluorescence data; and the image processing module is used for processing the fluorescence data to obtain a three-dimensional image of the to-be-detected particle. According to the scheme, three-dimensional scanning measurement of the high-enthalpy flow field without a sheet light source is realized, the measurement precision is improved, and the requirement on the energy of the laser is reduced.

A system and a method directed to a monitoring system of semiconductor processing chambers is provided. In particular, monitoring of any chemical formation on a chamber and a wafer of a semiconductor processing chamber using in-situ laser induced fluorescence is provided. The monitoring system and method detect issues before they become a problem for the semiconductor processing chambers by providing diagnosis on chamber health and mechanisms for associated process shifts with a faster turnaround time.

The invention provides a laser absorption and LIF imaging fused water quality COD detection method and system, and belongs to the technical field of water quality monitoring, the method comprises the following steps: placing a to-be-detected water sample in a sample pool, and vertically irradiating the water sample with laser; performing high-concentration COD detection by adopting an ultraviolet absorption spectrophotometry based on a Lambert-Beer law in combination with a calibration curve; a laser induced fluorescence (LIF) imaging technology is adopted, the RGB gray value of the ROI of a fluorescence image is extracted, and low-concentration COD detection is carried out in combination with a fluorescence intensity-COD model; automatically matching and detecting according to the concentration of the water sample, and outputting a final COD detection value; the system comprises a laser emission module, an image acquisition module, a sample detection module, a transmission light detection module and a data processing module. The advantages of two optical detection technologies are fused, rapid and accurate detection of COD of various water bodies is achieved, the system sensitivity is high, the structure is compact, and real-time monitoring of COD of the water bodies is achieved.

A sample preparation workflow to facilitate deep N-glycomics analysis of human serum by capillary electrophoresis with laser induced fluorescence (CE-LIF) detection accommodates the higher sample concentration requirement of electrospray ionization mass spectrometry connected to capillary electrophoresis (CE-ESI-MS). A temperature gradient denaturing protocol is applied on amine functionalized magnetic bead partitioned glycoproteins to avoid precipitation. This also results in the free sugar content of the serum being significantly decreased which allows PNGase F mediated release of the N-linked carbohydrates. The liberated oligosaccharides were tagged with aminopyrene-trisulfonate, utilizing a modified evaporative labeling protocol. This workflow provides appropriate amounts of material for example for use in CE-ESI-MS analysis in negative ionization mode.

The invention discloses a calibration device for a two-photon laser-induced fluorescence system, particularly relates to the technical field of laser calibration, and is technically characterized in that the device comprises an air pressure control device which is used for being connected with a vacuum calibration cavity to convey calibration gas into the vacuum calibration cavity and controlling the air pressure in the vacuum calibration cavity; the vacuum calibration cavity is used for placing calibration gas and receiving the laser light path, so that the calibration gas in the vacuum calibration cavity is excited to generate a fluorescent light path; the ionization device is used for ionizing hydrogen in the vacuum calibration cavity to obtain hydrogen atoms; and the detection device is used for receiving a fluorescent light path generated in the vacuum calibration cavity so as to complete calibration gas detection. A pair of discharge polar plates is installed in a vacuum calibration chamber and connected with an external high-voltage direct-current power supply through a high-voltage insulated wire, so that glow discharge is generated between the two discharge polar plates to ionize hydrogen molecules, and the ionized hydrogen molecules can form hydrogen atoms after being compounded to meet experimental calibration conditions.

The invention discloses a droplet microfluidic detection system for detecting pathogenic bacteria based on a deoxyribozyme probe, which belongs to the technical field of microbiological detection, and mainly comprises an automatic sample introduction device, a microfluidic droplet generation chip, a capillary tube-laser induced fluorescence detector and a signal acquisition and analysis device, the pathogen detection process comprises the following steps: respectively and automatically injecting an actual water sample, a deoxyribozyme probe, liquid drop oil, a pathogen lysis solution and a reaction buffer solution into the microfluidic liquid drop generation chip by the automatic sample injection device, stably generating liquid drops, and detecting the liquid drops through the capillary tube-laser induced fluorescence detector, and the signal acquisition and analysis device acquires and analyzes the fluorescence signal. The method has the advantages of high detection flux, high stability, high sensitivity, rapidness and the like, and can be applied to the field of environmental microbiological detection.

The invention discloses a method for detecting oligosaccharide in breast milk. The invention provides a breast milk oligosaccharide detection method, which comprises the step of detecting a sample to be detected by using a detection method based on capillary electrophoresis-laser induced fluorescence, and an operation buffer solution used in the detection method based on capillary electrophoresis-laser induced fluorescence comprises Tris-base, EDTA and urea, and PVP greater than 2% (m / V) and less than 4% (m / V) and boric acid greater than 5% (m / V) and less than 15% (m / V). The detection method provided by the invention can be applied to separation and detection of acidic and neutral HMOs isomers in to-be-detected samples (such as breast milk and infant formula milk powder samples).

A CE based method and kit for the determination of the size and purity of an AAV genome which relies on Capillary Electrophoresis-Laser Induced Fluorescence (CE-LIF) analysis. These methods and kits are capable of detecting intact and partial genomes in a virus vectors such as adeno-associated viruses as well as remove small size impurities. In one example, the method can include creating a nucleic acid ladder using CE-LIF, releasing the genome from within an adeno-associated virus, purifying said genome and analyzing said genome using CE-LIF and comparing the results of the analysis of the genome to the nucleic acid ladder to determine a size of nucleic acids in the genome.

A laser beam shaping and homogenizing optical system for flow cytometry analysis relates to the field of optical detection technology, in order to solve the problems existing in the prior art, the system comprises first lens L1, second lens L2, third lens L3, fourth lens L4 and protective glass L5 arranged in sequence along the light path direction; The front surface S1 of the first lens L1 is used as a diaphragm; The front surface S1 of the first lens L1 is aspherical, and the rear surface S2 is spherical; The front surface S3 of the second lens L2 is spherical, and the rear surface S4 is spherical; The front surface S5 of the third lens L3 is spherical, and the rear surface S6 is spherical; The front surface S7 of the fourth lens L4 is spherical, and the rear surface S8 is spherical; The front surface S9 of the protective glass L5 is plane, and the rear surface S10 is plane. It is mainly applied to flow cytometer, flow imaging flow analysis platform, laser induced fluorescence detection system, laser particle counter and other biomedical detection equipment which need linear uniform light spot illumination.

A touchless system for assessing a status of a target individual may include an optical component configured to image a patient sample, a Raman spectrometer subsystem configured to detect inelastically scattered light from the patient sample, and a laser-induced fluorescence spectrometer subsystem configured to detect fluorescence emissions of the sample; and a controller configured to receive the inelastically scattered light from the Raman spectrometer subsystem, receive the fluorescence emissions from the laser-induced fluorescence spectrometer subsystem, and determine a status of the sample based on the inelastically scattered light and the fluorescence emissions.