6 results about "Laser-induced fluorescence" patented technology

The invention provides a laser absorption and LIF imaging fused water quality COD detection method and system, and belongs to the technical field of water quality monitoring, the method comprises the following steps: placing a to-be-detected water sample in a sample pool, and vertically irradiating the water sample with laser; performing high-concentration COD detection by adopting an ultraviolet absorption spectrophotometry based on a Lambert-Beer law in combination with a calibration curve; a laser induced fluorescence (LIF) imaging technology is adopted, the RGB gray value of the ROI of a fluorescence image is extracted, and low-concentration COD detection is carried out in combination with a fluorescence intensity-COD model; automatically matching and detecting according to the concentration of the water sample, and outputting a final COD detection value; the system comprises a laser emission module, an image acquisition module, a sample detection module, a transmission light detection module and a data processing module. The advantages of two optical detection technologies are fused, rapid and accurate detection of COD of various water bodies is achieved, the system sensitivity is high, the structure is compact, and real-time monitoring of COD of the water bodies is achieved.

A sample preparation workflow to facilitate deep N-glycomics analysis of human serum by capillary electrophoresis with laser induced fluorescence (CE-LIF) detection accommodates the higher sample concentration requirement of electrospray ionization mass spectrometry connected to capillary electrophoresis (CE-ESI-MS). A temperature gradient denaturing protocol is applied on amine functionalized magnetic bead partitioned glycoproteins to avoid precipitation. This also results in the free sugar content of the serum being significantly decreased which allows PNGase F mediated release of the N-linked carbohydrates. The liberated oligosaccharides were tagged with aminopyrene-trisulfonate, utilizing a modified evaporative labeling protocol. This workflow provides appropriate amounts of material for example for use in CE-ESI-MS analysis in negative ionization mode.

The invention discloses a droplet microfluidic detection system for detecting pathogenic bacteria based on a deoxyribozyme probe, which belongs to the technical field of microbiological detection, and mainly comprises an automatic sample introduction device, a microfluidic droplet generation chip, a capillary tube-laser induced fluorescence detector and a signal acquisition and analysis device, the pathogen detection process comprises the following steps: respectively and automatically injecting an actual water sample, a deoxyribozyme probe, liquid drop oil, a pathogen lysis solution and a reaction buffer solution into the microfluidic liquid drop generation chip by the automatic sample injection device, stably generating liquid drops, and detecting the liquid drops through the capillary tube-laser induced fluorescence detector, and the signal acquisition and analysis device acquires and analyzes the fluorescence signal. The method has the advantages of high detection flux, high stability, high sensitivity, rapidness and the like, and can be applied to the field of environmental microbiological detection.

The invention discloses a method for detecting oligosaccharide in breast milk. The invention provides a breast milk oligosaccharide detection method, which comprises the step of detecting a sample to be detected by using a detection method based on capillary electrophoresis-laser induced fluorescence, and an operation buffer solution used in the detection method based on capillary electrophoresis-laser induced fluorescence comprises Tris-base, EDTA and urea, and PVP greater than 2% (m / V) and less than 4% (m / V) and boric acid greater than 5% (m / V) and less than 15% (m / V). The detection method provided by the invention can be applied to separation and detection of acidic and neutral HMOs isomers in to-be-detected samples (such as breast milk and infant formula milk powder samples).

A CE based method and kit for the determination of the size and purity of an AAV genome which relies on Capillary Electrophoresis-Laser Induced Fluorescence (CE-LIF) analysis. These methods and kits are capable of detecting intact and partial genomes in a virus vectors such as adeno-associated viruses as well as remove small size impurities. In one example, the method can include creating a nucleic acid ladder using CE-LIF, releasing the genome from within an adeno-associated virus, purifying said genome and analyzing said genome using CE-LIF and comparing the results of the analysis of the genome to the nucleic acid ladder to determine a size of nucleic acids in the genome.

A laser beam shaping and homogenizing optical system for flow cytometry analysis relates to the field of optical detection technology, in order to solve the problems existing in the prior art, the system comprises first lens L1, second lens L2, third lens L3, fourth lens L4 and protective glass L5 arranged in sequence along the light path direction; The front surface S1 of the first lens L1 is used as a diaphragm; The front surface S1 of the first lens L1 is aspherical, and the rear surface S2 is spherical; The front surface S3 of the second lens L2 is spherical, and the rear surface S4 is spherical; The front surface S5 of the third lens L3 is spherical, and the rear surface S6 is spherical; The front surface S7 of the fourth lens L4 is spherical, and the rear surface S8 is spherical; The front surface S9 of the protective glass L5 is plane, and the rear surface S10 is plane. It is mainly applied to flow cytometer, flow imaging flow analysis platform, laser induced fluorescence detection system, laser particle counter and other biomedical detection equipment which need linear uniform light spot illumination.