Two-laser induced fluorescence multi-color detector used for single-cell electrophoretic chip

Abstract

The invention discloses a two-laser induced fluorescence multi-color detector used for a single-cell electrophoretic chip.The detector is composed of a laser excitation module, a fluorescence collection and detection module, a microimaging module, a signal collection and processing module and a microfluidic chip detection platform and has the functions of performing two-laser induced fluorescence multi-color detection, normal microscopic observation, two-laser spot focusing and detection area alignment observation and the like on a microfluidic chip.By means of the detector, simultaneous detection and quantification of fluorescence substances with different light absorption and fluorescence characteristics, especially fluorescence labeling multi-component activated small molecules, with different excitation and different emission, in single cells of electrophoretic separation of the microfluidic chip can be achieved, and space of relative research and application of laser induced fluorescence detection, the microfluidic chip, single-cell analysis, detection of activated small molecules and the like is greatly expanded.

Description

technical field [0001] The invention relates to a dual laser-induced fluorescence multicolor detector for single-cell electrophoresis chips, especially for the "different excitation, different emission" fluorescently labeled multi-component active small molecules in single cells separated by microfluidic chip electrophoresis The simultaneous detection and quantitative analysis belongs to the field of chemical analysis instruments and also belongs to the field of microfluidic chip detection technology. Background technique [0002] The life process of cells requires the coordinated participation of a variety of active small molecules. In order to discover the physiological mechanisms that were covered up or could not be found in the past due to statistical detection in the study of cell populations, and to further study the physiological and pathological processes of cells, it is necessary to quantitatively measure single molecules at the same time. The content of various act...

AI Technical Summary

Problems solved by technology

[0004] However, in the face of the rapid development of microfluidic chips and the simultaneous quantitative detection of multi-component small molecules in single cells in many fields, the laser-induced fluorescence detectors currently used in microfluidic chips are not suitable for "different light absorption, different Simultaneous detection and quantification of multi-color fluorescent labeling substances with "fluorescent properties" or "different excitation, different emission"

The reason is that most of the reported laser-induced fluorescence detectors are based on the design of single-wavelength laser excitation and single-color fluorescence detection. There is also no report on the simultaneous quantitative determination of three or more multicolor fluorescently labeled small molecules in a single cell

In addition, the alignment of the focused laser and the separation channel of the chip in the existing laser-induced fluorescence detection is mostly based on the subjective judgment of the tester, and it is difficult to ensure the detection sensitivity and stability.

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