161 results about "Microscopic imaging" patented technology

This application belongs to the field of fluorescence microscopy imaging, and more specifically, relates to a dual-gel system suitable for dilatational microscopy of large-volume biological tissues. This dual-gel system achieves uniform expansion and morphological stability of tissue samples through a dilatational gel, and then imparts high mechanical strength using a stabilizing gel. This allows millimeter-scale large-volume biological tissue samples to possess both high expansion ratio and excellent mechanical stability, achieving 3-5 times linear expansion with a Young's modulus of not less than 300 kPa. It can stably adapt to vibrational cutting operations and support cutting of 100-500 μm thick layers, with intact cut surfaces and good interlayer consistency, greatly improving the efficiency and quality of three-dimensional data acquisition. Furthermore, the hydrogel sections obtained from the cutting have intact morphology and can be used as independent slides for multidimensional analysis. This application overcomes the limitations of traditional dilatational microscopy techniques in terms of sample volume and mechanical stability, providing an efficient and reliable solution for the three-dimensional reconstruction of millimeter-scale biological tissues.

This invention discloses a multi-functional optical microscopy imaging system that integrates multiple imaging techniques, including fluorescence lifetime imaging, bright-field microscopy, Raman microscopy, and one sample manipulation technique, optical tweezers, in a single microscope setup. This allows for the acquisition of structural, functional, and molecular information from the sample with simultaneous sample manipulation function. Various design approaches have been outlined for a multi-modality microscopy by segmenting the entire operational wavelength range of an objective lens into separate spectral channels, each dedicated to a specific optical technique.

The application discloses a multi-point focusing fitting microscopic imaging plane leveling method, system and device, which is used for high-precision automatic leveling of an imaging plane. The method comprises the following steps: establishing a two-dimensional sampling grid based on a measured plane, and determining grid node coordinates; establishing a space coordinate system, and calling calibration data to obtain a calibration image plane; continuously collecting an image sequence covering the two-dimensional sampling grid; dividing the image sequence according to the grid node coordinates to obtain a grid image sequence; calculating the definition of the grid image sequence by using a no-reference definition evaluation method to obtain a definition sequence; establishing an index relationship between the grid node coordinates and the maximum definition in the corresponding definition sequence to obtain a definition point set; performing plane fitting based on the definition point set to obtain a global focal plane model; calculating parallel parameters of the global focal plane relative to the calibration image plane according to the global focal plane model; and adjusting the posture of a placement platform of the measured plane through the parallel parameters.

This invention belongs to the field of fluorescence microscopy imaging technology, specifically a calculation method for high-density fluorescence fluctuation super-resolution microscopy imaging. This method dynamically controls the activation-quenching cycle of reversible fluorescent molecules through alternating sequential irradiation with activation and excitation light, effectively reducing the density of fluorescent molecules by combining activation light control and image differential processing techniques. Specifically, it includes: optimizing light control parameters such as frame rate, activation light power and exposure time, and excitation light power and exposure time; using an inter-frame difference algorithm to reduce the effective fluorescence density; and employing the SOFI algorithm for high-order cumulant calculation to achieve super-resolution reconstruction. Compared to existing technologies, this invention eliminates the need for complex multicolor labeling, significantly improves imaging resolution through a light control-differential synergistic strategy, and is compatible with various microscopy systems such as wide-field and confocal microscopy, providing an efficient and reliable technical solution for live-cell super-resolution observation.

The invention relates to a microfluidic device (1) for automatic microscopic imaging of bioorganisms, wherein the device (1) comprises a plurality of chambers (2) configured to receive individual bioorganisms, wherein the microfluidic device (1) comprises an alignment portion (3) arranged at a predetermined position and orientation with respect to the chambers (2), wherein the alignment portion (3) comprises a plurality of markers arranged in a pattern (5), wherein the markers (4) of the pattern (5) are arranged with respect to a central point (6) of the pattern (5) such that for any position of a field of view comprising only a fraction of the pattern (5), an automatic centering procedure of the field of view toward or to the central point (6) of the pattern (5) is derivable. The invention further relates to microscope systems for automatic microscopic imaging of bioorganisms, a method for automatized microscopic imaging of bioorganisms using a microscope system, a computer program as well as a method for manufacturing the microfluidic device (1) for automatic microscopic imaging of bioorganisms.

This invention discloses a laser image signal-to-noise ratio (SNR) enhancement method based on single-camera dual-channel imaging, relating to the fields of optical microscopy, digital image processing, and machine learning. The method includes: acquiring the original image from a single camera; separating the original image into a green channel image and a red channel image; constructing a denoising model based on the linear relationship between the green and red channel images, using the minimization of background noise in the green channel image as the objective loss function of the denoising model, and solving for an optimized parameter set; processing the green and red channel images according to the optimized parameter set to obtain a high SNR laser image after removing fluorescence background noise. This invention can remove fluorescence background noise from the original image acquired by a single camera without increasing hardware costs, effectively eliminating spectral crosstalk in the laser channel and significantly improving the laser image signal-to-noise ratio.

The application provides a chip-scale package interconnection structure failure analysis method and device, comprising: determining the failure external pin corresponding to the failure CSP package interconnection structure according to the current-voltage characteristic curve corresponding to each external pin; collecting and analyzing the time-domain reflection waveform of the failure external pin by using the electro-optical terahertz pulse reflection technology, positioning the target interconnection failure area in the interconnection path where the failure external pin is located according to the amplitude, time delay and waveform distortion characteristics of the reflection pulse; scanning the target interconnection failure area by using the three-dimensional X-ray microscopic imaging technology, and determining the target position of the failure point on the interconnection path where the failure external pin is located and the corresponding structural defects based on the scanning result. The application realizes the non-destructive failure analysis of the CSP package interconnection structure by combining the electro-optical terahertz pulse reflection technology and the three-dimensional X-ray microscopic imaging technology, and improves the failure point fault positioning accuracy and efficiency.

The application relates to a parallel scanning imaging method based on a planar super-resolution lens array, and relates to the technical field of fluorescence microscopic imaging, wherein the method comprises the following steps: placing a biological sample to be measured on a sample stage; controlling the sample stage to move the biological sample to be measured, performing full-field pre-scanning on the biological sample to be measured, synchronously collecting images of the biological sample to be measured during the full-field pre-scanning by means of an image collection module, and obtaining a pre-scanning panoramic image; dividing the pre-scanning panoramic image into a plurality of blocks, dividing different blocks into fine scanning areas and coarse scanning areas, and finally obtaining a super-resolution image corresponding to the fine scanning areas and a low-resolution image corresponding to the coarse scanning areas; and fusing the super-resolution image and the low-resolution image to obtain a fluorescence image of the biological sample to be measured. The application realizes the imaging requirements of the biological sample in super-resolution observation and low-exposure protection.

This application provides a dynamic tool setting device and method for diamond micro-end milling cutters, relating to the field of precision and ultra-precision machining technology. The method includes the following steps: acquiring a time-series pulse sequence of time-division multiplexing images; and performing tool setting detection on the diamond micro-end milling cutter based on the time-series pulses. This application utilizes the diffraction interference imaging principle of diamond micro-end milling cutters under coaxial laser illumination, employing object-side telecentric vision imaging to ensure a fixed holographic image magnification and microscopic imaging accuracy. By setting the camera trigger acquisition pulse through an external trigger controller via a host computer, the rotating image of the diamond micro-end milling cutter is sampled in a time-division multiplexing manner, achieving full-contour holographic imaging of the diamond micro-end milling cutter under rotating conditions. Simultaneously, by designing a downsampling holographic autofocus algorithm in holographic image processing, the computational load for image holographic reconstruction is reduced, accelerating the tool setting calculation efficiency and providing high-precision tool setting technology support for ultra-precision micro-milling.

Microscopy imaging that allows for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (High Density-SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ localization and identification (by in situ sequencing or sequential fluorescence hybridization) of rolonies derived from rolling circle amplification of circular oligonucleotides and in vitro sequencing of target amplified RNA or DNA in combination with SUMI identification at a subcellular level with no optical diffraction limitation in the amount of amplified target information that can be analyzed per cell. Apart from amplified RNA or DNA, the High Density-SUMI-Seq method can also be applied using linear oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.

The application provides a microscopic imaging detection device for micro-hole inner wall layer identification, comprising a microscopic imaging unit, a displacement driving unit, an image processing unit and a light path adjusting unit; the light path adjusting unit comprises a light source or an optical fiber, an aperture, a lens and a light splitting structure, which is used for guiding the illumination light into the imaging light path of the microscopic imaging unit and into the micro-hole along the optical axis. The image processing unit is used for determining the plate surface position according to the plate surface image and calculating the micro-hole aperture, controlling the light path adjusting unit to adjust the illumination light path parameters according to the micro-hole aperture, controlling the displacement driving unit to change the relative position step by step along the micro-hole depth direction, and determining the copper layer starting position, ending position and thickness according to the annular feature and depth position information in the different depth images. The application can improve the copper layer identification accuracy and stability in the micro-hole, and provides the depth basis for secondary drilling or hole expansion.

This application discloses an illumination rotation module arranged in a structured light super-resolution illumination microscopic imaging optical system. It includes a rotatable galvanometer with two parallel and opposite mirror surfaces arranged relative to a rotation axis intersecting the principal optical axis. M mirror groups, each comprising at least three mirrors, are arranged around the galvanometer. When the galvanometer is at an angular position about the rotation axis, linearly polarized light incident on the illumination rotation module is reflected sequentially through one mirror surface of the galvanometer, each mirror in a mirror group corresponding to the angular position, and the other mirror surface of the galvanometer. This causes the propagation direction and deflection direction of the linearly polarized light to rotate synchronously before exiting the illumination rotation module. This application also discloses a structured light super-resolution illumination microscopic imaging optical system.

The utility model discloses a kind of needle grinding machines, the needle grinding machine includes main body, work platform, fixed module and movement module;Work platform is installed on main body;Movement module includes movement platform and the drive portion that can drive movement platform movement;The position of work platform corresponding to movement platform is provided with work window;Fixed module is set on work platform, and movement platform can be driven in drive portion in work window movement to carry out grinding to the probe of probe card;First image module and second image module are further provided on work platform;First image module includes the first mechanical arm being set on work platform, the microscopic imaging device being set on first mechanical arm and first display screen;Second image module includes the camera being set on work platform and second display screen.The first image module and second image module of the present application can display the specific grinding condition of the probe of probe card in the grinding process from multiple angles, thereby providing accurate reference for user.

This invention belongs to the field of precision measurement technology, specifically a multi-focal-plane superimposed microscopic imaging simulation system based on inverse geometric ray tracing. The system includes: a system parameter configuration module for setting various parameters matching the actual multi-focal-plane superimposed microscopic measurement system, achieving precise alignment between the simulation system and the actual system; and a ray tracing module, based on an inverse geometric ray tracing algorithm, combining a lens model and Gaussian imaging formula to constrain the spatial conjugate relationship between the object and image, realizing sequential imaging simulation of a multi-focal-plane superimposed microscopic measurement system at a specific depth of field. This invention can efficiently and accurately simulate the sequential imaging process of multi-focal-plane superimposed microscopic measurements, providing scientific guidance for measurement algorithm verification and optical system hardware optimization, and broadening the application prospects of multi-focal-plane superimposed microscopic measurement technology.

The application discloses a monitoring method and system for three-dimensional phenotype parameters of stoma cells of living plants, and comprises the following steps: S1, a plant confocal three-dimensional microscopic imaging and photosynthetic parameter measuring device is used, a self-made photosynthetic leaf chamber is used to realize cooperative monitoring of a down-positioned confocal microscope and a portable photosynthetic instrument, morphological changes of the same stoma under different environments are monitored, and three-dimensional Z-stack images and physiological parameters of a stoma complex of the living plants are synchronously obtained; and S2, a fluorescence image is automatically segmented and three-dimensional surface reconstruction is performed by using a granularity-based active contour model, so that three-dimensional phenotype parameters and pore characteristics of the stoma complex are obtained.

This application relates to the field of microscopic imaging radiation calibration technology, providing a photon flux standard source and a method for measuring photon flux responsivity. The photon flux standard source includes an integrating sphere light source and an aperture; the outer side of the integrating sphere light source outlet includes a first flat surface with a first area, and the side of the aperture near the integrating sphere light source has a second flat surface with a second area. The first flat surface and the second flat surface are parallel and have a planar gap. The integrating sphere light source outlet has an average brightness within a solid angle defined by the aperture; wherein the planar gap, the average brightness, the first area, and the second area are used to calculate the photon flux to determine the photon flux responsivity of the detector under test. This application can accurately determine the photon flux entering the microscopic imaging system, thus improving the accuracy of the photon flux responsivity of the microscopic imaging system calibrated based on the photon flux.

The present application discloses a multiphoton microscopic imaging probe, an imaging device, and an imaging method. The imaging probe comprises: a medium assembly, wherein the medium assembly comprises a solid elastic medium and an imaging window plate that carries the solid elastic medium and is used for being in contact with an imaging object; a handle-shaped housing, wherein the medium assembly is detachably arranged at a first end of the handle-shaped housing; and an imaging module based on miniature laser scanning microscopy technology, wherein the imaging module is arranged in the handle-shaped housing, connected to a photoelectric composite cable and used for transmitting and controlling exciting light and receiving a fluorescence signal; and the imaging module comprises an objective lens in contact-fit with the solid elastic medium. Therefore, the objective lens provided by the present application comes into contact with the imaging object on the basis of the solid medium, eliminating the limitations of conventional immersion media on microscopic imaging apparatuses, thereby allowing the imaging probe to come into contact with the imaging object at any angle.

This invention provides a method for processing live single-cell extraction based on deformation trends, comprising the following steps: Step S1: Controlling a microfluidic chip to immerse itself in a culture medium containing adherent cells while maintaining a preset gap, controlling the injection flow rate of the injection channel and the aspiration flow rate of the aspiration channel to form a microjet acting on the cells; Step S2: Controlling an image processing module to receive and process cell morphology images on the culture substrate acquired by a microscopic imaging module, and calculating deformation parameters based on the comparison between the cell area in the current frame image and the initial cell area; Step S3: Comparing the deformation parameters with critical deformation parameters obtained through experimental calibration, and when the deformation parameters are greater than the critical deformation parameters, controlling a fluid drive module to reduce the injection flow rate and / or the aspiration flow rate; or, calculating a comprehensive damage risk index based on the deformation parameters, and when it is greater than 1, reducing the injection flow rate and / or the aspiration flow rate.

The application discloses a kind of microstructure ultra-precision machining in-situ measurement and error compensation method and system, it is related to ultra-precision machining and measurement technical field, comprising: using the optical microscopic imaging module integrated on machine tool, in-situ obtain the low-resolution microscopic image of the microstructure surface of workpiece clamping state under processing, and the pixel size calibration of optical microscopic imaging module is carried out;Low-resolution microscopic image is input into the super-resolution reconstruction network based on local implicit image function framework and is carried out super-resolution reconstruction, and high-resolution reconstruction image is generated according to the set arbitrary magnification;Pixel-level edge detection and sub-pixel fitting are carried out again, and the actual measurement size of microstructure key geometry is calculated;Based on the deviation of design target size and actual measurement size, compensation amount is calculated, and the compensation amount is converted into tool path correction amount under the same clamping reference and is implemented in-machine reprocessing, and geometric error closed-loop compensation is realized.The application can improve the efficiency and size consistency of ultra-precision machining.

The application discloses a reflection type super-large depth of field super-resolution wide-field optical microscopic system and method, and the system comprises a sample displacement module, a ring-shaped focusing light beam generation module, a light beam scanning module, a super-large depth of field super-resolution wide-field optical microscopic imaging module, an optical microscopic imaging module and a computer. Through the advantages of the ring-shaped focusing light beam illumination, the high focusing efficiency of the traditional lens and the high scanning speed of the light beam, efficient and fast super-long focal depth super-resolution focusing scanning illumination is realized, and then the traditional optical microscopic system can be used to realize fast, super-large depth of field super-resolution wide-field optical microscopic imaging. The application can be applied to non-labeled super-resolution microscopic fast imaging of biological samples, and can also be applied to industrial related super-resolution microscopic detection, real-time imaging of micro-operation and other fields.

1. Name of the product in this design: Live Imaging Organ Fixation Instrument. 2. Purpose of this design: To fix organs or tissues of live experimental animals during microscopic imaging. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

Disclosed in the present invention are a window and a microscopic imaging system. The window comprises a transparent glass slide and an immersion medium, the side of the transparent glass slide away from a sample to be observed is provided with the immersion medium, and the material of the immersion medium comprises silica gel. The microscopic imaging system provided by the present invention is provided with the window. Because the non-fluid silica gel of which the refractive index matches the refractive index of biological tissues is used as the immersion medium in the window, the window can be suitable for biopsy, thereby providing an excellent in-vivo imaging effect and product use experience.

This invention relates to the field of microscopic observation technology, specifically to an extended depth-of-focus microscopic imaging system incorporating optical tweezers functionality. The system includes a beam generation and combining module, a signal acquisition module, a signal separation module, an image reconstruction module, and an optical tweezers analysis module. The system generates a phase-modulated extended depth-of-focus illumination beam and an optical tweezers manipulation beam, which are then spatially coaxially combined to form a combined beam, which is then guided into the microscope objective to act on the sample region. The acquired optical signal is divided into independent detection channels through beam splitting processing. The imaging channel, combined with a depth-of-field extension point spread function model, optimizes the fusion algorithm to reconstruct the three-dimensional structure of the sample. The optical tweezers monitoring channel analyzes the optical path signal, calculates the three-dimensional position of particles, trapping stiffness, and optical force, and visualizes the three-dimensional relative relationship between these two parameters by combining the imaging results. This scheme achieves integrated design of the imaging optical path and the optical tweezers optical path, optimizes image reconstruction quality under large depth of field, and enables synchronous and collaborative operation of microscopic imaging and optical manipulation.

The utility model relates to biological tissue sample microscopic imaging technical field provides a slice formula three -dimensional tomography optical microscopic system, it includes ultrathin section module, optical imaging module and data acquisition module, ultrathin section module is used for realizing to sample's scanning formula section, ultrathin section module includes processing groove, cutter assembly, processing liquid circulating device and flush device, cutter assembly has the upper surface of slanting upwards, and the tissue slice obtained after sample is sliced via cutter assembly is exported from the upper surface;The liquid inlet of processing liquid circulating device is set up in the section export direction corresponding the upper surface, and processing liquid circulating device is used for forming the water flow suction field on the upper surface;The water outlet of flush device corresponds the upper end surface of sample and is set up towards cutter assembly's direction, and flush device is used for forming the water flow impact force along section export direction on the upper end surface of sample and the upper surface, the accuracy and reliability of the system to biological sample three -dimensional structure information acquisition are improved.

The imaging assembly bearing device, base station and microscopic image acquisition device and adjusting method comprise an imaging lens support, an imaging lens clamping seat and an adjusting guide seat; the bottom of the imaging lens clamping seat is movably connected between the imaging lens support and the adjusting guide seat; the levelness of the upper surface of the bottom of the imaging lens clamping seat relative to the bottom of the imaging lens support can be adjusted until the microscopic imaging assembly fixed on the imaging lens clamping seat is perpendicular to the upper surface of the bottom of the imaging lens clamping seat. With the structural design of the imaging assembly bearing device and the combination of the screw fastener, the distance adjusting fastener can improve the distance adjustment accuracy, the angle adjustment of the angle α between the imaging assembly and the ideal optical axis is more accurate, the distance L2 in the optical axis direction is more accurate, and the high-precision optical adjustment process is realized.

1. Name of the product in this design: Scientific Camera (Lux). 2. Application of this design: This design is used for collecting microscopic images in cell biology and electrophysiology experiments, capturing biophoton-related fluorescence spectra, directly imaging EUV / soft X-rays in high-energy physics, recording solar physics, orbit tracking and other astronomical phenomena. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.