Super-resolution confocal microimaging method and device based on column polarization vortex beam

Abstract

The invention provides a super-resolution confocal microimaging device based on a column polarization vortex beam. The super-resolution confocal microimaging device comprises a pinhole filter, a collimating lens, a polarization and phase transformation system, a pupil filter, an optical filter, a detector and a three-dimensional translational platform, wherein a beam emitted from a laser passes the pinhole filter to obtain a Gauss basic mode beam, the collimating lens collimates the Gauss basic mode beam into the parallel beam, the polarization and phase transformation system allows the parallel beam to pass and obtain the column polarization vortex beam with preset polarization and phase distribution, the pupil filter allows the column polarization vortex beam to pass and to be reflected through a beam splitter and focused on a to-be-detected sample through a collecting lens, light signals reflected from the sample pass the collecting lens and the beam splitter to be incident on the optical filter, and the optical filter only allows a fluorescence signal of the light signals to be transmitted, the fluorescence signal is focused on a detecting pinhole through the collecting lens and is detected and converted, through the detector, into an electrical signal to be output, the sample is placed on the three-dimensional translational platform, detection of the sample at different positions can be realized by moving the three-dimensional translational platform, and accordingly three-dimensional scanning imaging of the sample can be realized.

Description

technical field [0001] The invention relates to confocal microscopic imaging technology, in particular to a method and device for super-resolution confocal microscopic imaging using cylindrically polarized vortex beams. Background technique [0002] The development of modern biology and material science has put forward higher and higher resolution requirements for the study of microstructure, hoping to reveal the physical essence of life processes and material properties from the molecular level. However, limited by the optical diffraction limit, the horizontal resolution of ordinary optical microscopes can only reach 200nm, and the vertical resolution is about 500nm, which is powerless for the study of subcellular and molecular structures. Although electron microscopy (Electron Microscopy), atomic force microscopy (Atom Force Microscopy), near-field scanning optical microscopy (Near-field Scanning Optical Microscope, NSOM) and other techniques can obtain high resolution, du...

AI Technical Summary

Problems solved by technology

However, the ability of this adjustment to improve the resolution is still very limited, because the wavelength of the incident light cannot be reduced infinitely, and the numerical aperture of the imaging objective lens is also limited.

Research in recent years has shown that the measurement resolution of the confocal system can be further improved by adopting pupil filtering, 4Pi illumination and interference technology, but the improvement effect is not very obvious, so it is necessary to find a new technological breakthrough

Images