3970results about "Microscopes" patented technology

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

The invention provides a microscopic automatic focusing method and system based on image gray histogram characteristics, and the method comprises the steps: collecting an image sequence under different focal lengths, carrying out the fuzzy processing, extracting a gray histogram of each frame of image, and calculating the peak position and full width at half maximum of the histogram as the evaluation characteristics of the image definition; calculating the variance of each feature and automatically allocating a weight according to the relative response degree; and finally, comprehensively evaluating the image definition through a weighted definition scoring function, and selecting the focal length corresponding to the image with the optimal score as the optimal focusing position. The method is based on the global features of the gray histogram, is high in anti-noise capability, is adaptive to different imaging scenes through weight adaptive adjustment, is low in calculation complexity, supports real-time focusing, is especially suitable for high-noise fluorescence microscopic imaging scenes, is high in system portability, is low in operation threshold, and effectively improves the accuracy and stability of microscopic automatic focusing.

The invention discloses a method for clearly imaging a mask under an optical objective, and relates to the technical field of optical detection and image processing, and the method comprises the following steps: S1, constructing a light intensity distribution model based on the boundary condition of the field of view of the optical objective in combination with the reflection characteristic and incident angle change rule of a metal edge material, and determining the coverage range of metal edge reflection crosstalk, generating a mask boundary interference prediction map; s2, according to the mask boundary interference prediction map, performing region division on the micro-reflectivity image, extracting brightness gradient characteristics of reflectivity lifting in an interference region, and generating a brightness gradient parameter set required by image filtering; the method is based on light intensity modeling, fusion direction filtering, pixel stripping, gradient reconstruction and credibility weighted fusion, realizes closed-loop control from interference prediction to pixel restoration, has high resolution and adaptivity, can accurately strip edge reflection artifacts and restore a real image structure, remarkably reduces misjudgment and rework rate, and is suitable for large-scale popularization and application. And the mask yield and the stability of the detection system are improved.

Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.

A platform for characterizing biomolecules, biological tissue sections, viruses, or other particles of interest in a non-invasive, high-throughput, cost-effective, and label-free manner. The platform includes a merged set of imaging modalities including and capable of interferometric microscopy and wide-field or confocal microscopy (such as, for example, a wide-field super-resolution surface enhanced Raman spectroscopy, SERS), although some aspects may be employed with data sets only received from wide-field or confocal microscopy. Related devices, systems, methods, techniques, and variations to the same are also provided.

Methods and devices for analyzing a sample from a subject are provided. A device includes one or more coherent light sources and one or more partially coherent light sources. The light sources are configured to emit first and second wavelengths of electromagnetic radiation towards an imaging chamber configured to hold blood cells. The light sources are aligned to illuminate a single common area of the imaging chamber. The device includes optics positioned to receive light from the imaging chamber, and a detector in optical communication with the optics. The detector is configured to detect fluorescence emission and backscatter from blood cells when present in the imaging chamber. The device includes mixers / unmixers configured to replicate and mix first and second signals representative of the responses to the first and second wavelengths after interacting with the plurality of blood cells to generate spectro-spatial responses.

This disclosure provides methods for spatial profiling of biological analytes present in a biological sample. Methods include generating feature arrays using a master / copy format using recessed arrays, and methods for using such arrays. For example spatially-tagged analyte capture analytes can be used in spatial detection in methods to determine the location of analytes (e.g., proteins) in biological samples.

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

The invention provides a focusing method, device and equipment. The method comprises the following steps: determining pixel offset between a red channel image and a green channel image of a tissue slice; the red channel image and the green channel image are a red channel image and a green channel image of a tissue slice in a ghosting image captured by the color image sensor when a red light filter, a green light filter and double optical wedges in the focusing module are modulated at the same time; determining an out-of-focus distance according to the pixel offset and a preset corresponding relation; the preset corresponding relation is the corresponding relation between the pixel offset and the defocus distance; and focusing according to the out-of-focus distance. According to the method provided by the embodiment of the invention, the problem that positive and negative defocusing directions cannot be distinguished through a single-frame image in a phase shift detection automatic focusing technology based on image monochromatic double-hole modulation is solved, and the problem of time waste caused by high-speed switching of an illumination light source in an automatic focusing technology based on red-green double-LED illumination is solved; and meanwhile, the problem that a phase shift detection automatic focusing technology based on pupil segmentation images is relatively small in focusing range is solved.

Methods for determining a location of a feature in a spatial array with features include: (a) providing an array with a first set of one or more features immobilized on a substrate, a first feature of the first set having a first barcoded oligonucleotide with a first spatial barcode and a first constant sequence, and a second set of one or more features immobilized on the substrate, a second feature of the second set having a second barcoded oligonucleotide with a second spatial barcode and a second constant sequence; (b) attaching the first constant sequence to the second constant sequence to generate a nucleic acid product; (c) determining all or a portion of a sequence of the nucleic acid product or a complement thereof; and (d) associating the second barcoded oligonucleotide with the first barcoded oligonucleotide in the nucleic acid product.

The application discloses a multi-marked endoscopic imaging lens for digestive tract, and belongs to the field of biomedical microscopic endoscopic imaging. The endoscopic imaging lens comprises a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens and a seventh lens; the first lens focuses laser; the second lens is matched with the first lens to limit a light beam in an effective light aperture of the lens; the third lens corrects chromatic aberration; the fourth lens and the fifth lens are combined to correct aberration; the sixth lens adopts an aspheric lens to correct spherical aberration caused in a collimating and focusing system; and the seventh lens is matched with the sixth lens to correct chromatic aberration. The working spectral range of the microscopic objective lens group is 488-860 nm, the effective light aperture is less than 1.8 mm, the mechanical outer diameter is not greater than 2.6 mm, the numerical aperture at the object side or the tissue is 0.55, the imaging field of view is 450 microns, the magnification is 2.14 times, and the working distance is 84 microns. The effect of matching excitation and fluorescence spectrum of a clinically available fluorescence contrast agent is realized.

The invention discloses a parallel imaging OCT (Optical Coherence Tomography) system based on a metasurface area array, which relates to the technical field of medical instruments, is small in size and can realize intraocular single-shot area array imaging and a three-dimensional position depth perception function by using metasurface and optical coherence tomography imaging information in a sample tissue with a limited size. According to the technical scheme, a light beam emitted by a laser is divided into two paths through a beam splitter, and the two paths enter a sample arm module and a reference arm module respectively; the sample arm module is a probe and comprises a multi-core optical fiber and a first metasurface; the reference arm module comprises a collimator, a motor and a right-angle reflector; the probe enters a sample tissue, a light beam is focused on the sample tissue and then is backscattered, the backscattered light beam reversely passes through the first metasurface, the multi-core optical fiber, the beam splitter, the second metasurface, the third lens, the grating and the focusing lens and then forms a spectral line beam on the CCD sensor, and the computer reads a signal from the CCD sensor and performs three-dimensional reconstruction imaging on the sample tissue.

The present inventive concept relates to a method and a device for training a machine learning model to construct a digital color image depicting a sample. The method comprising: acquiring a training set of digital images of a training sample by: illuminating, by a plurality of white light emitting diodes, the training sample with a plurality of illumination patterns, and capturing, for each illumination pattern of the plurality of illumination patterns, a digital image of the training sample; receiving a ground truth comprising a high-resolution digital color image of the training sample, wherein a resolution of the high-resolution digital color image is relatively higher than a resolution of at least one digital image of the training set of digital images; and training the machine learning model to construct the digital color image depicting a sample using the training set of digital images and the ground truth. The present inventive concept further relates to a microscope system and a method for constructing a digital color image depicting a sample.

The present invention relates to a microscope comprising a light source, an illumination beam path for guiding excitation light to a sample, a detector for detecting emission light emitted by a sample, a detection beam path with a microscope objective for guiding the emission light to the detector, a mechanical drive for setting a relative lateral position between the sample and the microscope objective, and a control unit.The microscope is characterized in that the control unit is configured to perform a setting step, wherein the mechanical drive is sequentially adjusted to at least three different relative lateral positions; a detection step, wherein measurement data from the detector are acquired for at least a subset of points in the sample within a field of view of the detection beam path, wherein for each point of the subset measurement data are acquired for at least two different lateral positions of the mechanical drive; and an evaluation step, wherein field inhomogeneity in the field of view and microscopic sample information are extracted from the measurement data. In further aspects, the invention relates to a method for determining field inhomogeneity in a field of view of a microscope and to a microscopy method.

In some embodiments, a method for classifying elements of a blood sample is provided, the method including: digitally staining an image of the blood sample using a trained machine learning model to generate a digitally stained image; extracting one or more intermediate features generated by the trained machine learning model during the digital staining of the image; providing the one or more extracted intermediate features to a trained multi-class classifier; and employing the trained multi-class classifier to classify at least one element in the blood sample based on the one or more extracted intermediate features. Many other embodiments are also provided.

Methods for determining a location of a feature on an array include: (a) providing a first array with a first plurality of features immobilized on a first substrate; (b) providing a second array with a second plurality of features immobilized on a second substrate; (c) aligning the first array with the second array; (d) hybridizing a first barcoded oligonucleotide of the first array to a second barcoded oligonucleotide of the second array, thereby producing a combined nucleic acid that includes first and second spatial barcodes; (e) determining all or a portion of the sequence of the combined nucleic acid; and (f) identifying the second barcoded oligonucleotide associated with the first barcoded oligonucleotide in the combined nucleic acid, and determining the location of a second feature in the second array.

The invention provides a linear optical tweezer astigmatism field regulation and control method based on an optical transmission matrix. The design method comprises the following steps: establishing a 4 * 4 optical matrix for generating a linear optical tweezer astigmatism field and a transmission equation; analyzing the regulation and control rule of the parameters of the optical element on the astigmatism characteristics; collecting a fluorescence slice image of the initial astigmatic light field through an axial tomography scanning method; reconstructing the spatial distribution of the initial image light field; judging whether the light field accords with a preset astigmatism characteristic or not, if not, determining the astigmatism characteristic to be optimized, and adjusting element parameters according to a regulation and control rule; and acquiring the adjusted image of the astigmatism light field again, reconstructing the image, and continuously performing judgment iteration until the image accords with the preset astigmatism characteristic, thereby realizing regulation and control of the astigmatism light field. Matrix optics is used in optimization of a linear optical tweezer image dispersion light field optical system, the limitation of traditional trial and error design is avoided, the method can be applied to the fields of light control technology, light sheet fluorescence microscopic imaging and the like, and the performance of the optical system is improved.

The invention belongs to the technical field of biological sample detection, and particularly relates to a microscanning and classifying all-in-one machine for a cell pathology dyeing sealing sheet and a dyeing method of the microscanning and classifying all-in-one machine for the cell pathology dyeing sealing sheet. Comprising a machine body, a top feeding assembly, a dyeing assembly, a lifting and transferring assembly, a glue dripping assembly, a piece sealing assembly, a microscope scanning assembly and a stacking and storing assembly. According to the method, isochronous standardized dyeing is adopted, all sample slide plates are further arranged before each cycle, the dyeing time of each step is equal accurate time, large-flux standardized dyeing can be performed, and a standardized dyeing method which is high in flux, high in speed, good in dyeing effect and accurate in time is formed. And meanwhile, micro-scanning is integrated into a whole to form a full-automatic assembly line with a small-space large-flux function and full feeding and discharging convenience.

The invention relates to a multifunctional on-site material evidence investigation and evidence obtaining system based on an intelligent robot dog, and belongs to the technical field of criminal investigation. According to the system, a robot dog is driven by a communication device and a positioner to reach a trace detection site, and collected trace detection image data are transmitted to a main control device; attitude precision adjustment is carried out through a joint encoder arranged on the angle driving framework in combination with the collected trace detection image data; calculating the actual distance between the microscope and the target trace through a trace detection distance model; based on the actual distance, the focusing driving framework is controlled to move in combination with the corresponding initial focusing parameters; and performing real-time definition evaluation on the acquired trace detection image through a definition evaluation framework, recording an optimal focusing position and related trace detection image data after the evaluation is passed, and updating a database. The multi-scene application of trace detection is realized, the problem of blurring of the trace detection image caused by environmental interference or sample movement is solved, and the focusing quality and the shooting success rate of trace detection shooting are effectively improved.

Methods for determining a location of a feature on a spatial array include (a) providing an array of features on a substrate, where a feature of the array includes a barcoded oligonucleotide having, in a 5′ to 3′ direction, a spatial barcode, a cleavage domain, and a constant sequence; (b) hybridizing a priming oligonucleotide to the constant sequence; (c) extending the priming oligonucleotide using the barcoded oligonucleotide as a template; and (d) determining all or a portion of a sequence of the extended priming oligonucleotide corresponding to the spatial barcode, or a complement thereof, and a location of the extended priming oligonucleotide, and using the location of the extended priming oligonucleotide to determine the location of the feature on the spatial array.

The present invention relates to a vessel holder fixed with a conical screw for use in a biological microscope or the like, and more particularly, to an improved vessel holder fixed with a conical screw to be accurately fixed to a reference side without a change in position with respect to impact vibration while allowing the vessel holders to be closely attached to an upper surface of the stage with only one screw, thereby improving usability, cell observation efficiency and accuracy.

The invention belongs to the technical field of infrared optics, and discloses a long-wave infrared continuous zooming microscope lens. The lens is provided with two lenses, namely a first lens and a second lens which are sequentially and coaxially arranged between an object plane and an image plane; the first lens is a meniscus positive lens of which the convex surface faces the image side, and the second lens is a meniscus positive lens of which the convex surface faces the image side; the first lens and the second lens can move between an object plane and an image plane so as to carry out high and low magnification switching. The long-wave infrared continuous zooming microscope lens can realize multi-magnification switching, and also has the characteristics of small number of lenses, large target surface and clear imaging; the working wave band is 8-12 [mu] m, the magnification factor is 0.2-0.6, the F number of the image space is 1.5, the number of pixels is 1280 * 1024, and the size of the pixels is 12 [mu] m.

A device for light field microscopy, comprising: a two-dimensionally spatially resolving detector for detecting light radiated by a sample; a detection beam path having at least one microscope objective lens; and at least one multi-lens array for imaging the light radiated by the sample onto the detector, wherein the multi-lens array is arranged in a plane which is optically conjugated with respect to a rear focal plane of the microscope objective lens, or in the vicinity of such a plane; and a control and evaluation unit for activating the detector and for evaluating the measurement data from the detector. The device also includes an adjustable relay optical system in order to image the rear focal plane of the microscope objective lens into the plane in which the multi-lens array is arranged, or in the vicinity of this plane. A method for light field microscopy is also provided.

This invention presents a frequency-domain fluorescence lifetime imaging system, enhancing capabilities with a spatial beam attenuator and pinhole beam splitter. The spatial beam attenuator reshapes any beam into a circular Gaussian beam profile, being compact, cost-effective, with low loss and back reflection. Using digital printing technology, a thin optical film is deposited onto an optical substrate for precise local attenuation of the beam in either transmission or reflection mode. The pinhole beam splitter consists of an optical beam splitter with an active region the size of a pinhole, positioned near the beam's focus, which directs focused light into two paths based on acceptance or rejection by the pinhole. This configuration allows for two detectors to collect photons from both paths concurrently, facilitating dual-mode imaging. Consequently, the system can generate both confocal and conventional non-confocal laser scanning fluorescence lifetime images of the sample at the same time.

A system for optical interrogation of tissue samples, the system including: a microtome configured to section one or more tissue sections from a tissue block, the one or more tissue sections including one or more tissue samples; a transfer medium configured to gather the one or more tissue sections and to transfer the one or more tissue sections to one or more slides; and an optical interrogation system including an illumination system configured to illuminate the one or more tissue sections and an imaging system configured to perform an imaging analysis on the one or more tissue sections illuminated with the illumination system.

The invention discloses a spore microscopic imaging automatic focusing method and system based on a target recognition algorithm, and the method comprises the steps: analyzing the image focusing effect of spore images collected under different object distances in the process of changing the object distance of a microscope, and determining the optimal positive focus object distance based on the object distance corresponding to the spore image with the optimal image focusing effect; the image focusing effect is cooperatively determined based on the number of spores which can be recognized by using a target recognition algorithm in the image and a focusing evaluation value obtained by analyzing the image by using a focusing evaluation function; the image focusing effects of different images are preferentially determined according to the number of the spores which can be recognized; and when the number of the spores is consistent and the image focusing effect difference cannot be determined, further determining the image focusing effects of different images based on the focusing evaluation values of the images. According to the method, the problem of focal plane misjudgment caused by complicated background interference of micro-particle impurities such as pollen and dust in a spore microscopic image is solved.

The invention relates to a structured light illumination confocal super-resolution measurement system and method, and relates to the technical field of optical measurement. The system comprises a digital micromirror device (DMD), a sample platform, an electric displacement platform, a microscopic imaging assembly, a complementary metal oxide semiconductor (CMOS) imaging assembly and computer equipment, and the DMD is used for adjusting the point light source array and the structured light field. According to the DMD-based confocal super-resolution measurement method, a structured light field and a signal-noise separation technology are combined, and high-precision and high-resolution three-dimensional surface reconstruction can be realized. By using the flexibility of the DMD and the advantages of the super-resolution technology, the spatial resolution and speed of confocal imaging are effectively improved, so that the measurement process is more accurate. In addition, low-rank decomposition and self-supervised deep learning methods are fused, the image reconstruction process is optimized, noise interference is suppressed, and the overall performance of the system is further improved.

The invention relates to the technical field of optical imaging, and discloses a novel reflective dark field imaging method and system based on Fourier conjugate plane modulation, and the method comprises the steps: controlling a first spatial light modulator to load a dynamic annular illumination pattern, and projecting the dynamic annular illumination pattern to a rear focal plane of an objective lens; light field distribution formed by scattering signal light and annular reflected light is separated on a rear focal plane by using an objective lens; relaying the light field distribution to a conjugate plane where a second spatial light modulator is located through a second relay light path; and controlling the second spatial light modulator to load a dynamic blocking pattern, blocking the annular reflected light and enabling the scattered signal light to pass through, and finally imaging. According to the invention, two electronically synchronous spatial light modulators are adopted to construct a dynamic illumination light field and realize adaptive spatial filtering respectively, so that cooperative matching of an illumination pattern and a blocking pattern is realized, active compensation of optical chromatic aberration is facilitated, the problem of background leakage under wide-spectrum illumination is solved, and the illumination quality is improved. And a high-numerical aperture objective lens lacking a dark field channel is compatible.

A method and apparatus for calibrating an imaging system provides a program stored in the imaging system having a graphical user interface and providing a plurality of tools to align hardware components of the imaging system including cameras for imaging cell culture vessels received by the imaging system and having a tool for providing a video image output of a cell culture vessel received in the imaging system.