522results about "Microscopes" patented technology

In various aspects, microscope systems are described, comprising or corresponding to optical microscopes, such as for example a transillumination microscope or a fluorescence microscope. The microscope systems may be used independently or configured with or integrated into another device or system, such as an imaging mass spectrometer or imaging mass cytometer. In various embodiments of this aspect, the microscope system is used to obtain one or more images of a sample, such as at a variety of different light collection wavelengths and / or for a variety of different illumination conditions. The microscope systems can employ various optical components and configurations allowing for high throughput imaging, such as components for imaging the sample at suitable resolutions with a wide field of view and / or for spatially separating different detection channel wavelengths from light output (e.g., transmitted or emitted light) from the sample.

Provided is a cell culture vessel which allows resolution to be high in a Z-axis direction. A cell culture vessel (1) for accommodating a cell or cell tissue therein includes: a frame part (11) which is provided so as to be located at positions that correspond to respective sides of a polyhedral shape; a window part (12) which is light-transmissive and which is provided so as to be located at positions that correspond to a respective plurality of faces out of faces of the polyhedral shape; and a shaft part (17) which extends, from any of vertices of the polyhedral shape, outward in a direction that is not parallel to any of lines normal to the faces of the polyhedral shape.

The application discloses a three-degree-of-freedom microscopic visual precision motion platform, which comprises a linear slide, a worm reducer, a workbench and an optical microscope system. The linear slide is connected with an air floating vibration isolation platform through an aluminum profile stand. The worm reducer is fixed on the upper surface of an aluminum alloy platform carried by the linear slide. One workbench is fixed on each end of the output shaft of the worm reducer. A microstructure workpiece is installed on the lower end workbench, and a machining workpiece is installed on the upper end workbench. The optical microscope is installed on the upper end of an industrial camera from bottom to top. The industrial camera is fixed on the air floating vibration isolation platform through a supporting rod gear clamp, a rack supporting rod and a differential lifting platform. The three-degree-of-freedom microscopic visual precision motion platform can place the optical microscope system below the microstructure workpiece, thereby providing machining space for the machining workpiece, and providing a new scheme for applying microscopic visual technology to the field of precision machining.

The present disclosure provides flow cell devices, systems, and methods for facilitating and performing DNA sequencing analysis with reduced system complexity and cost, significant cost of goods saving, and reduced contamination level. The sequencing systems described herein permit processing of multiple flow cells simultaneously, such that sequencing and imaging steps, or multiple sequencing methods, can be performed in parallel using a single sequencing system.

The application provides a continuous zoom optical system and a stereomicroscope for the stereomicroscope, and the optical lens unit comprises, in sequence along a main optical axis, an upper fixed lens group, a compensation lens group, a middle fixed lens group, a zoom lens group and a lower fixed lens group; a variable diaphragm is arranged between the middle fixed lens group and the zoom lens group along the main optical axis; the upper fixed lens group, the compensation lens group, the zoom lens group and the lower fixed lens group are all configured as double lens combinations, the focal length values of the upper fixed lens group and the zoom lens group are negative, and the focal length values of the compensation lens group and the lower fixed lens group are positive; the middle fixed lens group is configured as a double-concave single lens; the compensation lens group and the zoom lens group are axially moved along the main optical axis based on a first zoom fitting curve and a second zoom fitting curve respectively; and the advantages are that the system has smooth magnification, suppresses vignetting phenomenon, and dynamically adjusts the numerical aperture, the depth of field and the light throughput to adapt to different scene requirements.

This invention discloses a multi-functional optical microscopy imaging system that integrates multiple imaging techniques, including fluorescence lifetime imaging, bright-field microscopy, Raman microscopy, and one sample manipulation technique, optical tweezers, in a single microscope setup. This allows for the acquisition of structural, functional, and molecular information from the sample with simultaneous sample manipulation function. Various design approaches have been outlined for a multi-modality microscopy by segmenting the entire operational wavelength range of an objective lens into separate spectral channels, each dedicated to a specific optical technique.

The application discloses a multi-point focusing fitting microscopic imaging plane leveling method, system and device, which is used for high-precision automatic leveling of an imaging plane. The method comprises the following steps: establishing a two-dimensional sampling grid based on a measured plane, and determining grid node coordinates; establishing a space coordinate system, and calling calibration data to obtain a calibration image plane; continuously collecting an image sequence covering the two-dimensional sampling grid; dividing the image sequence according to the grid node coordinates to obtain a grid image sequence; calculating the definition of the grid image sequence by using a no-reference definition evaluation method to obtain a definition sequence; establishing an index relationship between the grid node coordinates and the maximum definition in the corresponding definition sequence to obtain a definition point set; performing plane fitting based on the definition point set to obtain a global focal plane model; calculating parallel parameters of the global focal plane relative to the calibration image plane according to the global focal plane model; and adjusting the posture of a placement platform of the measured plane through the parallel parameters.

This invention relates to the field of precision alignment technology, specifically to an astigmatic differential confocal alignment system and method capable of synchronous imaging. The astigmatic differential confocal alignment system includes: an astigmatic differential confocal optical path system and an imaging optical path system sharing the same objective lens; the astigmatic differential confocal optical path system includes a laser, a collimating and expanding beam system, a polarizing beam splitter cube, a quarter-wave plate, a long-pass filter, a short-pass dichroic mirror, an objective lens, a non-polarizing beam splitter cube, a plano-convex cylindrical lens, and two four-quadrant photodetectors connected in sequence; the imaging optical path system includes a visible light point source, a beam splitter, a short-pass dichroic mirror, an objective lens, a sleeve lens, and a camera connected in sequence; the two optical path systems are coaxial and synchronized. When the defocus error signal FES crosses zero, the camera synchronously acquires a clear morphological image of the surface of the sample under test, achieving synchronization of alignment and imaging.

The application provides a reflective imaging module and a reflective imaging device. The reflective imaging module comprises a shell, an LED light source, a mirror, an objective lens, an SPR chip unit and a camera. The LED light source is integrated on the outer side of the shell and is used for outputting light. The mirror is arranged in the shell in an inclined direction and is used for receiving the light output by the LED light source. The objective lens is connected to the shell and is used for receiving the light reflected by the mirror. The SPR chip unit is arranged on one side of the shell and comprises a transparent cover plate and an SPR chip. The transparent cover plate is arranged on one side of the SPR chip, and the SPR chip receives the light converged by the objective lens. The camera is arranged on the other side of the shell and is used for shooting the SPR chip unit and collecting a color picture. The shell is integrated with the LED light source, the mirror and the objective lens, the miniaturized design is realized, the mutual spacing between the shell and the LED light source, the mirror and the objective lens is avoided, and the occupied space of the reflective imaging module is reduced.

An imaging system uses a multi-view reflector (MVR) to simultaneously measure the three-dimensional (3D) position and orientation of a light emitter. The MVR is positioned at a back focal plane (BFP) of a light collecting optical system, and uses pyramid reflectors and / or conical reflectors to reflect light in at least two regions of the BFP to at least two detection channels of a detector. The at least two detection channels produce images that are used to determine the 3D position and orientation of a light emitter. The system may be used, for example, to image the structure of molecules or organelles in a cell, track the movement of molecules in a cell, or to study the interaction of molecules within a cell.

The utility model discloses a kind of dual-mode microscopes and its special components, including optical microscope component and lensless microscopy component, both form switchable dual-mode microscope, lensless microscopy mode is used for the preliminary screening of to-be-measured sample, optical microscope mode is used for the accurate observation of to-be-measured sample;Lensless microscopy component includes first light source, image sensor and image signal processor;In lensless microscopy mode, first light source and image sensor are respectively located on the two sides of to-be-measured sample on object table, when the light emitted by first light source passes through to-be-measured sample, image sensor receives optical signal and converts it into electrical signal, image signal processor converts electrical signal into interference or diffraction image, and it is restored into directly observable image.The dual-mode microscope of the utility model is switched by dual mode, while ensuring observation accuracy, greatly reduce workload, and there is no need to have too high requirement to the detection resolution of lensless microscopy.

Presented herein is an illumination assembly for providing polychromatic measurement light (230) for an optical measurement device. The illumination assembly includes an aperture component (202) having a first side and an opposing second side and at least one orifice (204), at least one pump light source (206) positioned to provide pump light (220) to the first side of the aperture component (202), and a photoluminescent component (208) positioned on the second side of the aperture component (202) for converting the pump light (220) receptive on the photoluminescent component (208) from the at least one pump light source (206) into polychromatic measurement light (230). At least a portion of the measurement light (230) can pass through the aperture component (202) through the at least one orifice (204) to provide the measurement light (230) to the first side of the aperture component (202). Also presented are related methods and measurement apparatus for using the lighting assembly.

The present invention provides a technology whereby relative positioning in the horizontal direction between a specimen observation area in a specimen container and an imaging field of view can be reliably performed, even prior to adjusting the focal position in the vertical direction using an auto-focus system. This specimen observation apparatus: obtains a luminance value for an image at a plurality of locations in the specimen container, prior to performing auto-focus; and uses the number of high-luminance regions and the width of those regions and identifies a central position, in the horizontal direction, in the specimen container or uses the number of low-luminance regions and the width of those regions and identifies the central position, in the horizontal direction, in the specimen container.

The application relates to a parallel scanning imaging method based on a planar super-resolution lens array, and relates to the technical field of fluorescence microscopic imaging, wherein the method comprises the following steps: placing a biological sample to be measured on a sample stage; controlling the sample stage to move the biological sample to be measured, performing full-field pre-scanning on the biological sample to be measured, synchronously collecting images of the biological sample to be measured during the full-field pre-scanning by means of an image collection module, and obtaining a pre-scanning panoramic image; dividing the pre-scanning panoramic image into a plurality of blocks, dividing different blocks into fine scanning areas and coarse scanning areas, and finally obtaining a super-resolution image corresponding to the fine scanning areas and a low-resolution image corresponding to the coarse scanning areas; and fusing the super-resolution image and the low-resolution image to obtain a fluorescence image of the biological sample to be measured. The application realizes the imaging requirements of the biological sample in super-resolution observation and low-exposure protection.

A method for measuring cellular mechanics in an in vitro fibrosis model comprises : (a) providing a plurality of microscope devices and assembling in a coaxial manner to form a coaxial microscope system for imaging; (b) placing the sample on a stage and scanning through the coaxial microscope system; (c) capturing a first image and a second image of the sample using the coaxial microscope system, generating the first image and the second image into a third image, and delineating cell boundary based on the third image; (d) defining a plurality of regions of interest in the third image; (e) determining the mechanical properties in the sample, including calculating a first elastic modulus of the cells and a second elastic modulus of protein in the regions of interest; and (f) obtaining the cell status in the sample, and comparing the first elastic modulus with the second elastic modulus.

This invention relates to a high numerical aperture, large field-of-view total internal reflection microscope objective in the ultraviolet wide band. Along the optical axis from image to object, it comprises: a first objective consisting of a first and a second reflecting mirror arranged sequentially along the optical axis OA from image to object, used to receive parallel light and form an intermediate image plane; and a second objective consisting of a third and a fourth reflecting mirror arranged sequentially along the optical axis OA from image to object, used to image the intermediate image plane onto the object plane. The first reflecting mirror is a convex mirror with negative optical power; the second reflecting mirror is a concave mirror with positive optical power; the third reflecting mirror is a concave mirror with positive optical power; and the fourth reflecting mirror is a concave mirror with positive optical power. This invention employs a four-mirror total internal reflection structure, and by optimizing the focal length and aperture ratios of each reflecting mirror, it achieves a balance between high numerical aperture and a large field of view, overcoming the limitations of traditional total internal reflection objectives where numerical aperture and field of view are mutually constrained.

A laser system for multiphoton microscopy comprises a seeder oscillator, fast modulator, amplifier, second modulator, and optical parametric amplifier configured to produce pulses with variable pulse spacing temporally synchronized to a scanner having sinusoidal motion. The variable pulse spacing ensures each pixel receives substantially the same number of laser pulses, eliminating uneven energy distribution of fixed repetition rate systems. The scanner operates at 4-12 kHz frequencies while the seeder oscillator produces femtosecond pulses at 10-100 MHz with 10-100 ns pulse spacing. The optical parametric amplifier outputs wavelengths from 600-1900 nm, with 1300-1700 nm optimized for three-photon microscopy. Methods include synchronizing pulse timing to scanner motion and varying interpulse intervals across scan lines. For 8 kHz scanners with 512 pixels per line, the system achieves 32% energy reduction compared to constant pulse spacing while maintaining 100-150 mW average power on biological samples, preventing thermal damage and enabling video rate imaging.

This invention provides a microscope device that can more accurately determine the three-dimensional refractive index distribution in a sample. [Solution] The microscope device includes a first microscope unit that irradiates a sample with first illumination light directed in a first direction and detects light from the sample corresponding to the irradiation of the first illumination light; a second microscope unit that irradiates the sample with second illumination light directed in a second direction different from the first direction and detects light from the sample corresponding to the irradiation of the second illumination light; and a data processing unit that generates a three-dimensional refractive index distribution in the sample based on the detection signal of light detected by the first microscope unit and the detection signal of light detected by the second microscope unit.

An objective attachment for a microscope objective for transporting an immersion liquid between a target region on a front side of the microscope objective and a line, comprising at least one cannula having a spout for dispensing and / or receiving the immersion liquid and having a distal opening, to which the line for the immersion liquid can be connected, and comprising a fastening device for holding the cannula on a housing of the microscope objective and for positioning the spout relative to the target region is described. The fastening device comprises at least two arms, which are configured to at least partially laterally engage around the housing of the microscope objective and to hold on the housing in a force-fitting manner. An apparatus for feeding immersion liquid to a target region on a front region of a microscope objective and / or for removing immersion liquid from the target region also is described.

The present invention is related to a method, a computer program, and an apparatus for adapting an estimator for use in a microscope for estimating a position of an emitter in a sample based on a method, in which the sample is illuminated with light at one or more sets of probe positions and fluorescence photons are acquired for the sets of probe positions. The invention is further related to a microscope, which makes use of such a method or apparatus. According to the invention, the sample is illuminated (S1) with light at one or more sets of probe positions and fluorescence photons are acquired (S2) for the sets of probe positions. Photon counts of the acquired (S2) photons are then added (S3) to vectors of photon counts or sums of photon counts are determined for the sets of probe positions. Subsequently, a value representative of background noise is determined (S4) from the vectors of photon counts or the sums of photon counts. The value representative of background noise is then used for adapting (S5) the estimator in real-time.

Techniques for measuring single synaptic signals under varying experimental conditions include controlling a video recording microscope to capture, at multiple different times, a first imaged area in a sample holder as a video frame when the sample holder is disposed on a stage and holds a sample of neuronal tissue combined with at least one fluorophore that emits a corresponding electromagnetic wavelength in a synapse during synaptic activity. At least one synaptic region of interest is determined based on a group of pixels in the first imaged area that record electromagnetic emissions from the fluorophore at the different times. An ordered time series of emission intensity is recorded in each of the synaptic region of interest. Peak emission intensity values in the time series are corrected for transmitter label transients. The ordered time series with corrected peak emission intensity values are stored in a data structure with a standard format.

To provide an optical system and a laser processing device with which it is possible to downsize the optical system of the laser processing device and heighten the safety of laser processing.SOLUTION: Provided is an optical system (30, 30A and 30B) for relaying light to a processing lens (26) which is used for processing a workpiece, the system comprising a spatial light modulator (24) and a second lens (L2) located between the spatial light modulator and the processing lens. When it is assumed that f2 represents the focal distance of the second lens and M represents the magnification of projection from the spatial light modulator to the processing lens pupil of the processing lens, the distance D from the second lens to the processing lens pupil is D=f2-Mf2, and the distance D1 from the spatial light modulator to the second lens is D1=f2-f2 / M, the spatial light modulator being in a conjugate relation with the processing lens pupil of the processing lens.SELECTED DRAWING: Figure 6

A method for an optical imaging system having a scanning imaging device includes obtaining sensor data from a detector of the scanning imaging device. The sensor data include a representation of a pattern captured by the detector. The method further includes determining a characteristic geometry of the representation of the pattern, comparing the characteristic geometry with a reference geometry in order to determine a comparison result, determining at least one calibration parameter for calibrating at least one control unit for moving a beam-conducting element of the scanning imaging device based on the comparison result, and operating the at least one control unit based on the at least one calibration parameter.

The application discloses a microscope positioning structure and a microscope. The microscope positioning structure comprises a base, a rotating shaft, an inclined rod, a containing cover, a positioning piece and an elastic piece. The rotating shaft is rotationally connected with the base, and a plurality of positioning grooves are formed in the circumferential direction of the rotating shaft. One end of the inclined rod is fixedly connected with the rotating shaft, and the other end of the inclined rod is connected with a lens of the microscope. The containing cover is fixedly connected with the base. The positioning piece is arranged in the containing cover and the base, and the positioning piece is moved along the extension direction of the positioning piece so that the positioning piece is in abutment or separation with the positioning grooves. The elastic piece is arranged in the containing cover, and the elastic piece is fixedly arranged between the containing cover and the positioning piece, so that the positioning piece has a moving trend towards the rotating shaft. The microscope comprises the above-mentioned microscope positioning structure. Through the above arrangement, the positioning piece can be prevented from being lost.

This application discloses an illumination rotation module arranged in a structured light super-resolution illumination microscopic imaging optical system. It includes a rotatable galvanometer with two parallel and opposite mirror surfaces arranged relative to a rotation axis intersecting the principal optical axis. M mirror groups, each comprising at least three mirrors, are arranged around the galvanometer. When the galvanometer is at an angular position about the rotation axis, linearly polarized light incident on the illumination rotation module is reflected sequentially through one mirror surface of the galvanometer, each mirror in a mirror group corresponding to the angular position, and the other mirror surface of the galvanometer. This causes the propagation direction and deflection direction of the linearly polarized light to rotate synchronously before exiting the illumination rotation module. This application also discloses a structured light super-resolution illumination microscopic imaging optical system.

To acquire focused optical information in a short period.SOLUTION: A scanning device includes: an observation optical system that radiates primary light toward one surface in order to acquire optical information related to at least some of a plurality of spots; a scanning section that performs main scanning in which the observation optical system moves relative to an array plate 101 in a first direction and acquires the optical information, and sub-scanning in which the observation optical system moves relative to the array plate 101 in a second direction intersecting the first direction without acquiring the optical information; and an adjustment section that adjusts the relative position of the observation optical system with respect to the array plate 101 in an optical axis direction of the primary light. The adjustment section performs the adjustment if the scanning section is in a sub-scanning period.SELECTED DRAWING: Figure 4

An auto-focusing system is disclosed. The system includes an illumination source. The system includes an aperture. The system includes a projection mask. The system includes a detector assembly. The system includes a relay system, the relay system being configured to optically couple illumination transmitted through the projection mask to an imaging system. The relay system also being configured to project one or more patterns from the projection mask onto a specimen and transmit an image of the projection mask from the specimen to the detector assembly. The system includes a controller including one or more processors configured to execute a set of program instructions. The program instructions being configured to cause the one or more processors to: receive one or more images of the projection mask from the detector assembly and determine quality of the one or more images of the projection mask.

A method of generating a binding surface for an object in a solution comprising the steps of: i. obtaining a blocked surface wherein the blocked surface comprises a base surface and at least one blocking reagent; ii. targeting of a portion of the blocked surface with a light source; and iii. irradiating the portion of the blocked surface with the light source under conditions which cause a quantity of the blocking reagent bound to the portion of the blocked surface to be removed, thus creating a binding surface on the base surface; wherein the binding rate of the object to the blocked surface is less than the binding rate of the object to the base surface is provided. A method of generating a binding surface for an object in a solution is also provided.

This utility model discloses a fixing and adjustment mechanism for a two-dimensional scanning galvanometer, belonging to the field of confocal microendoscopic imaging and detection technology. It employs a detachable and securely connected positioning seat and base. Corresponding arc-shaped grooves are provided on the positioning seat and base to form mounting holes, in which the galvanometer fixing shaft is positioned. This utility model has a simple and reliable structure, effectively ensuring the stability and adjustability of the confocal microendoscopic, and is easy to use in production practice.

An infinity-corrected microscope objective lens has, arranged in order from the object side along an optical axis, a first lens group having a positive refractive power, and a second lens group having a positive refractive power, an intermediate image forming plane in which light from an object forms an image being positioned between the first lens group and the second lens group, and the microscope objective lens satisfying the condition below. −0.2<f×NA / TL<−0.05, where f is the focal length of the microscope objective lens, NA is the object-side numerical aperture of the microscope objective lens, and TL is the distance on the optical axis from the lens surface of the microscope objective lens on the side thereof closest to the object to the lens surface of the microscope objective lens on the side thereof closest to the image.