6931results about "Microscopes" patented technology

A method of self-testing an imaging system of a sample handling apparatus is provided. Systems and non-transitory computer readable mediums performing the method are also provided.

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

This disclosure provides methods for spatial profiling of biological analytes present in a biological sample. Methods include generating feature arrays using a master / copy format using recessed arrays, and methods for using such arrays. For example spatially-tagged analyte capture analytes can be used in spatial detection in methods to determine the location of analytes (e.g., proteins) in biological samples.

Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

A surgical microscope lighting control system comprising a controller configured to: estimate or determine a lighting need of a surgeon during at least part of a surgical procedure based at least on a viewing status of a region of illumination, wherein the viewing status of the region of illumination is estimated or determined (a) based at least on whether or not the region of illumination is being displayed, or (b) based at least on tracking data of the surgeon or a head-mounted display; determine an adjustment to be made to an illumination provided by a lighting unit of a surgical microscope based on the lighting need; and in response to determining the adjustment, send a first signal to the lighting unit to change the illumination according to the adjustment.

The invention discloses a high-power microscope intelligent scanning method for a bone marrow smear, which comprises the following steps: S1, automatically dividing a scanning area: acquiring a bone marrow smear image by adopting a front camera, and selecting an optimal scanning area through a multi-dimensional quality evaluation model; s2, intelligent partitioning and focusing point distribution: dividing an optimal scanning area into N * M sub-areas, determining P focusing sampling points by adopting a layered sampling strategy, and establishing a three-dimensional coordinate mapping table; s3, multi-point focusing data acquisition: according to a preset coordinate mapping table, driving an objective table and an objective lens assembly to move to each sampling point in sequence, and constructing an N * M * P-dimensional focusing data matrix; s4, performing focal plane fitting by a least square method: performing three-dimensional plane fitting on the acquired data; and S5, intelligent scanning imaging: calculating the theoretical optimal focal length of each position in the scanning path according to the fitting function, and driving the objective lens assembly to adjust the focal length in real time for scanning imaging. According to the invention, the scanning area identification accuracy and the image definition uniformity are automatically improved in the whole process.

The invention provides a three-dimensional automatic focusing method and device based on multi-modal data fusion and Kalman filtering and a readable storage medium, and high-precision real-time focusing is realized through multiple steps: collecting sample three-dimensional phase information and different focal plane image data by using a phase and image sensor, and calculating phase depth and definition; constructing a multi-plane Kalman filtering cluster, modeling the focus state of each focal plane, and predicting the focus by fusing the neighborhood coupling influence; the focus state is updated and corrected in combination with the image definition and the phase gradient, and the optimal three-dimensional focus position is determined by means of a collaborative optimization decision maker; dynamically adjusting the noise matrix and the coupling coefficient, and controlling the objective lens to complete focusing. According to the method, through multi-modal fusion and multi-plane cooperation, the problems of focus drifting and jumping are effectively solved, the focusing precision and stability of an imaging system in a complex sample and a dynamic environment are remarkably improved, and the method is suitable for equipment such as a biomedical full-automatic microscope and a digital pathological section scanner.

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and / or DNA loci.

The present disclosure relates to a line-scanning based super-resolution imaging method and apparatus. A line-scanning based super-resolution imaging method includes: providing a plurality of line-type structured excitation lights, each of the plurality of line-type structured excitation lights having a different illumination pattern; illuminating a target area in a line-by-line scanning manner along a single first direction while switching the plurality of line-type structured excitation lights, such that each row in the target area is illuminated by a corresponding one of the plurality of line-type structured excitation lights; and detecting response light generated by each row in the target area in response to being illuminated by the corresponding one of the plurality of line-type structured excitation lights.

The invention provides a microscopic automatic focusing method and system based on image gray histogram characteristics, and the method comprises the steps: collecting an image sequence under different focal lengths, carrying out the fuzzy processing, extracting a gray histogram of each frame of image, and calculating the peak position and full width at half maximum of the histogram as the evaluation characteristics of the image definition; calculating the variance of each feature and automatically allocating a weight according to the relative response degree; and finally, comprehensively evaluating the image definition through a weighted definition scoring function, and selecting the focal length corresponding to the image with the optimal score as the optimal focusing position. The method is based on the global features of the gray histogram, is high in anti-noise capability, is adaptive to different imaging scenes through weight adaptive adjustment, is low in calculation complexity, supports real-time focusing, is especially suitable for high-noise fluorescence microscopic imaging scenes, is high in system portability, is low in operation threshold, and effectively improves the accuracy and stability of microscopic automatic focusing.

The invention provides a dynamic focusing method and system based on a light spot image and a medium. The method comprises the following steps: focusing to any surface of an object to be measured, and obtaining a calibration image; calculating the center of mass of the focusing surface light spot of the to-be-measured object in the calibration image as a zero point; the method comprises the following steps: processing a light spot image acquired on the surface of a to-be-measured object to acquire a corresponding pixel line-pixel value curve graph; through analyzing peak points and valley points of the pixel line-pixel value curve graph, screening segmentation points of the pixel line-pixel value curve graph, and calculating a corresponding light spot centroid according to the pixel line-pixel value curve graph on the left side and / or the right side of the segmentation points corresponding to the target layer of the object to be measured so as to obtain a defocusing amount; the dynamic focusing is realized by analyzing whether the defocusing amount falls into the control range. The dynamic focusing method provided by the invention is simple and convenient in calculation and convenient for hardware implementation; meanwhile, the invention further provides simplified segmentation point calculation, so that the calculation efficiency is improved; and determining the break point for ensuring the accuracy of dynamic focusing.

The invention discloses a method for clearly imaging a mask under an optical objective, and relates to the technical field of optical detection and image processing, and the method comprises the following steps: S1, constructing a light intensity distribution model based on the boundary condition of the field of view of the optical objective in combination with the reflection characteristic and incident angle change rule of a metal edge material, and determining the coverage range of metal edge reflection crosstalk, generating a mask boundary interference prediction map; s2, according to the mask boundary interference prediction map, performing region division on the micro-reflectivity image, extracting brightness gradient characteristics of reflectivity lifting in an interference region, and generating a brightness gradient parameter set required by image filtering; the method is based on light intensity modeling, fusion direction filtering, pixel stripping, gradient reconstruction and credibility weighted fusion, realizes closed-loop control from interference prediction to pixel restoration, has high resolution and adaptivity, can accurately strip edge reflection artifacts and restore a real image structure, remarkably reduces misjudgment and rework rate, and is suitable for large-scale popularization and application. And the mask yield and the stability of the detection system are improved.

Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.

A platform for characterizing biomolecules, biological tissue sections, viruses, or other particles of interest in a non-invasive, high-throughput, cost-effective, and label-free manner. The platform includes a merged set of imaging modalities including and capable of interferometric microscopy and wide-field or confocal microscopy (such as, for example, a wide-field super-resolution surface enhanced Raman spectroscopy, SERS), although some aspects may be employed with data sets only received from wide-field or confocal microscopy. Related devices, systems, methods, techniques, and variations to the same are also provided.

Methods and devices for analyzing a sample from a subject are provided. A device includes one or more coherent light sources and one or more partially coherent light sources. The light sources are configured to emit first and second wavelengths of electromagnetic radiation towards an imaging chamber configured to hold blood cells. The light sources are aligned to illuminate a single common area of the imaging chamber. The device includes optics positioned to receive light from the imaging chamber, and a detector in optical communication with the optics. The detector is configured to detect fluorescence emission and backscatter from blood cells when present in the imaging chamber. The device includes mixers / unmixers configured to replicate and mix first and second signals representative of the responses to the first and second wavelengths after interacting with the plurality of blood cells to generate spectro-spatial responses.

The utility model discloses a micro-fluidic chip imaging positioning device, and belongs to the technical field of micro-fluidic chip positioning. According to the technical scheme, the system comprises a microcosmic illumination module, a microcosmic imaging module, a chip moving and macroscopic illumination module, a macroscopic imaging module and an optical path multiplexing module; the microscopic illumination module comprises a fly's-eye lens assembly, the fly's-eye lens assembly comprises two fly's-eye lenses, the optical axes of the two fly's-eye lenses are parallel to each other, and the focus of each lens array element in one fly's-eye lens coincides with the center of the lens array element at the corresponding position of the other fly's-eye lens. The micro-fluidic chip clamp is applied to the aspect of micro-fluidic chip imaging positioning, overcomes the defects caused by an existing micro-fluidic chip clamp, solves the technical problems of universality, automation and high precision of micro-fluidic chip positioning, and has the characteristics of rapidness, automation, high precision and universality.

This disclosure provides methods for spatial profiling of biological analytes present in a biological sample. Methods include generating feature arrays using a master / copy format using recessed arrays, and methods for using such arrays. For example spatially-tagged analyte capture analytes can be used in spatial detection in methods to determine the location of analytes (e.g., proteins) in biological samples.

An image formation lens (IL) for a microscope comprises: a negative lens; and a positive lens (L13) that satisfies the following conditional expression:-0.002×(ν⁢dP-35)+0.602-θ⁢gFP<023<ν⁢dP<65where vdP: an Abbe number of the positive lens, and θgFP: a partial dispersion ratio of the positive lens that is defined by the following expression: θgFP=(ngP−nFP) / (nFP−nCP) where ngP is a refractive index of the positive lens to a g-line, nFP is a refractive index of the positive lens to an F-line, and nCP is a refractive index of the positive lens to a C-line.

A spatiotemporal illumination microscope system is presented. The system includes a multi-well plate configured to receive live cells and a DMD configured to deliver targeted illumination to selectively illuminate a plurality of the wells. The system further includes a tandem-lens system having an objective lens and an imaging lens positioned facing each other to capture fluorescent light emitted from the cells. The system also includes an image sensor that receives fluorescent light captured by the tandem-lens system, generating imaging data. The system further includes a controller configured to use the imaging data to control closed-loop modulation of the live cells.

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

The invention discloses a turntable-based dual confocal image scanning microscope and an imaging method, and relates to the technical field of optical elements, systems, instruments and imaging. The imaging light source assembly, the optical fiber transmission assembly, the light source collimation assembly, the structured light generation assembly, the turntable assembly, the microscopic amplification assembly and the camera detection assembly are arranged along a light path; the structured light generation module realizes structured light illumination on a sample surface by loading a specific mask sequence, super-resolution imaging is realized, pinholes in the turntable assembly are equidistantly arranged according to an Archimedes spiral, and the turntable assembly rotates at a high speed to realize multi-point parallel scanning and de-scanning; the detection module performs detection by using an area array detector, so that the resolution improvement of double diffraction limit is realized; according to the dynamic pinhole array-pixel redistribution method, super-resolution reconstruction is carried out based on an image scanning microscopic system principle, and Stokes shift, optical aberration and other non-ideal conditions can be effectively corrected.

The invention provides a light spot position detection method, an automatic focusing method, a related system and a storage medium. A first light source is controlled to emit a first light beam to irradiate a to-be-detected sample, and a focusing sensor is controlled to receive the first light beam reflected from the to-be-detected sample; acquiring pixel data generated when the focusing sensor senses a light spot, formed on the focusing sensor, of the first light beam, and determining the center pixel position of the light spot based on the pixel data so as to determine the position of the light spot. According to the invention, the position of the light spot can be determined, so that the automatic focusing system can perform automatic focusing based on the position of the light spot, and the image definition is improved. Besides, in the invention, the light spot position is determined by using the central pixel position in the pixel data sensed by the focusing sensor, and the central pixel position can represent the central position of the light spot, so that the light spot position determination accuracy can be relatively high by determining the light spot position based on the central pixel position; therefore, the accuracy of automatic focusing and the image definition are improved.

The invention provides an automatic focusing system, an automatic focusing method and a surface defect detection method, and the method comprises the steps: controlling a plurality of light sources in a light source array to be lightened one by one according to a preset time sequence, and enabling a light beam emitted by each light source to be reflected by a corresponding to-be-detected sub-region on the surface of a to-be-detected device, and one part of the reflected light is detected as a defocus detection light spot by the defocus information acquisition unit, and the other part of the reflected light forms a detection image on the main detection camera. As the position of the defocus detection light spot and the position of the microscope objective have a mapping relation, defocus information corresponding to the defocus detection light spot can be obtained through the mapping relation, and therefore automatic focusing of the microscope objective is achieved. According to the lighting sequence of the light source, automatic focusing and imaging are sequentially carried out on each to-be-detected sub-region, so that the image detection precision can be improved, the light path hardware requirement can be reduced, the height information of the surface of the to-be-detected device can be obtained through defocusing information, surface defect detection on an object with complex three-dimensional morphology characteristics is facilitated, and the detection efficiency is improved. And the detection flexibility and precision are improved.

The invention discloses a four-channel fluorescence detection light path module of a super-resolution biological microscope, which divides a fluorescence signal of an intermediate image plane (5) into four conjugate channels (7a-7d) through multi-stage dichroscope light splitting, reflector guiding and lens focal plane adjustment on the premise of not introducing additional aberration, and realizes real-time synchronous detection by using a single large-target-surface camera (8). By adjusting the distance between the reflectors, the inclination angle of the parallel plate glass or the position of the lens, the device can adapt to different camera target surface sizes and compensate chromatic aberration, and the problems of motor switching delay and dual-channel aberration in the prior art are solved.

Systems, methods, and devices for providing a labeling technique for microscope slides using laser-markable ink are described. In some embodiments, laser-markable ink may be applied to a microscope slide or a sheet comprising a plurality of microscope slides. A laser may be provided to a portion of the laser-markable ink to initiate a chemical reaction in the laser-markable ink. The chemical reaction may mark a surface of the microscope slide at the portion of the laser-markable ink. The surface of the microscope slide may be marked with information and / or machine-readable indicia without etching the surface of the microscope slide.

The invention relates to the technical field of microscopes, in particular to a stereomicroscope which comprises an eyepiece, an interpupillary distance adjusting assembly, an optical hinge set, a prism steering set, a distributed reflection light path assembly and a continuous zoom objective lens set. The distributed back-shooting light path assembly comprises a negative lens group, a positive lens, a negative lens and a positive lens group, the negative lens group is arranged between the prism steering group and the continuous zoom objective lens group, the positive lens is arranged between the optical hinge group and the prism steering group, and the negative lens and the positive lens group are arranged between rectangular prisms in the optical hinge group; a negative lens group, a positive lens group, a negative lens group and a positive lens group are sequentially arranged among all parts of the stereomicroscope, so that the space layout of a mechanical structure is combined, the aperture of a light beam is adjusted, interception of the prism to the light beam is reduced, and off-axis vignetting is reduced; the technical problems of large off-axis vignetting and poor imaging quality caused by the fact that a light path passes through a plurality of groups of prisms in a stereomicroscope in the prior art are solved, and the overall imaging requirement is met.

The present disclosure provides systems, devices, and methods for imaging and imaging subject. The systems and devices may include one or more light filtering devices. The light filtering devices may include a chamber having an input and an output. The chamber may be configured to receive a beam of light. The chamber may comprise a plurality of reflectors configured to direct the light received in an optical path from the input to the output via reflectance of the light between one or more reflectors of the plurality of reflectors. The chamber may be configured to reject out of focus light along the optical path.

A flying-over beam pattern scanning hologram microscope device includes: a scan beam generation unit which converts a first beam and a second beam to a first spherical wave and a second spherical wave, and then allows the first and second spherical waves to interfere with each other to form a scan beam; a scanning unit, which comprises a scan mirror for controlling the scan beam in the horizontal direction, and a translation stage for moving an object in the vertical direction at the rear end of the projection unit; the projection unit projecting the scan beam onto an object plane; and a light collection unit for detecting a beam that has passed through the objective lens again after fluorescing or being reflected from an object.