Multifocal imaging systems and method

a multi-focal imaging and imaging system technology, applied in the field of multi-focal imaging systems and methods, can solve the problems of reducing the resolution of the resulting image, affecting the usefulness of tissue images, and cross-talk that can occur, so as to achieve fast imaging of the material

a multi-focal imaging and imaging system technology, applied in the field of multi-focal imaging systems and methods, can solve the problems of reducing the resolution of the resulting image, affecting the usefulness of tissue images, and cross-talk that can occur, so as to achieve fast imaging of the material

US20070057211A1Inactive Publication Date: 2007-03-15MASSACHUSETTS INST OF TECH
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  • Multifocal imaging systems and method

Examples

Experimental program
Comparison scheme
Effect test

case 1

[0165] The normalized inverse of this intensity image (from a uniform fluorescent dye) is multiplied with the yx images taken of the sample. The resulting images are then displayed and saved as a normalized image.

case 2

[0166] A large number of images from a sample at various positions (and thus with a random underlying intensity structure) is averaged. This image is then inversed and normalized. This image is multiplied with the original data is then displayed and saved as a normalized image.

case 3

[0167] A simplified image is generated which consists of 36 sub-images (generated by the 6×6 foci). Each of the sub-images carries the average intensity generated by the specific foci. For example, all 32×32 pixels in the top left sub image carry the same number; 45. The image is then inversed and normalized. This image multiplied with the original data is then displayed and saved as a normalized image. An image can be generated either from the intensity image generated by the process of case 1 (fluorescent image) or case 2 (over many images averaged). 3D xyz image normalization is carried out in a similar fashion as in case 2 of the xy image normalization. A z-intensity profile (an example is FIG. 28b) is generated by averaging the intensity signal the xy planes form different positions in z. As the penetration depth increases, the average intensity decreases along the z-axis. In order to get a good average intensity for the z-intensity profile, images from a sample at various pos...

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Abstract

In the systems and methods of the present invention a multifocal multiphoton imaging system has a signal to noise ratio (SNR) that is reduced by over an order of magnitude at imaging depth equal to twice the mean free path scattering length of the specimen. An MMM system based on an area detector such as a multianode photomultiplier tube (MAPMT) that is optimized for high-speed tissue imaging. The specimen is raster-scanned with an array of excitation light beams. The emission photons from the array of excitation foci are collected simultaneously by a MAPMT and the signals from each anode are detected using high sensitivity, low noise single photon counting circuits. An image is formed by the temporal encoding of the integrated signal with a raster scanning pattern. A deconvolution procedure taking account of the spatial distribution and the raster temporal encoding of collected photons can be used to improve decay coefficient. We demonstrate MAPMT-based MMM can provide significantly better contrast than CCD-based existing systems.

Description

CROSS REFERENCE TO RELATED APPLICATIONS [0001] This application claims the priority of U.S. Provisional Application No. 60 / 684,608 filed May 25, 2005 entitled, MULTI FOCAL MULTIPHOTON IMAGING SYSTEMS AND METHODS, the whole of which is hereby incorporated by reference herein.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT [0002] N / A BACKGROUND OF THE INVENTION [0003] Systems and methods for microscopic analysis of biological material have been used for characterization and diagnosis in many applications. Fluorescence microscopy, for example, has been used for optical analysis including the histological analysis of excised tissue specimens. Optical coherence tomography has been used for three dimensional imaging of tissue structures, however, the limited resolution of existing systems has constrained its use for definitive pathological analysis. Confocal microscopy has been used for high resolution imaging and has controllable depth of field but limited imaging speed. ...

Claims

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Application Information

Patent Timeline
15 Mar 2007
Publication
US20070057211A1
IPC
G03B42/08
CPC
G01N21/6452; G01N21/6458; G01N21/6486; G02B21/16; G02B21/0032; G02B21/0076; G02B21/002
Inventors
BAHLMAN, KARSTEN; KIM, KI-HEAN