15 results about "Multiphoton imaging" patented technology

Methods, systems and adaptors for multiphoton imaging of, and laser-tissue interactions with, non-transparent tissue, such as ocular and periocular tissue, are provided. Aspects of the present invention include methods of imaging a structure through non-transparent tissue, such as ocular or periocular tissue, comprising: deploying an excitation source to transmit light energy to a structure through non-transparent tissue, such as ocular or periocular tissue, detecting light emitted from the structure via multiphoton excitation through the non-transparent tissue, such as ocular or periocular tissue, and imaging the structure based on the detected light. Aspects of the present invention further include methods of treating tissue, such as ocular or periocular tissue or dermatologic tissue, by manipulating an imaged structure, including, for example, providing non-incisional therapy, photo-tissue interactions, multi-photon-mediated damage, such as thermal damage, photo-disruption, photo cross-linking, blood vessel coagulation or ablating tissue. Aspects of the present invention further include methods of imaging dermatologic tissues or providing dermatologic treatments. Aspects of the present invention further include methods of providing cosmetic treatments. Aspects of the present invention further include methods of guiding delivery of gene therapy or cellular therapy in ocular or periocular tissue. Also provided are systems and adaptors for performing the methods described herein.

The present disclosure provides a multiphoton imaging method. The method includes a) immersing a semi-submersible microscope objective in a liquid medium that is at least one of scattering or absorbing; b) directing laser light through the semi-submersible microscope objective and into the liquid medium in an image-wise manner under conditions such that multiphoton absorption by the multiphoton absorber occurs, and at least partial polymerization of the polymerizable compound occurs resulting in an article; and c) removing uncured polymerizable compound to clean the article. The liquid medium includes a polymerizable compound, a secondary component, and a multiphoton absorber. An article is also provided. The article includes a material defining one or more tortuous or arcuate channels, one or more internal architectural voids, one or more undercuts, one or more perforations, or combinations thereof, at least one of which exhibits a surface roughness of 1.0 micrometer Ra or less.

A multimodal imaging system based on a radio-frequency programmable GHz femtosecond pulse train. The system comprises: a femtosecond pulse laser light source (101), a pulse splitting module (102), a pulse amplification module (103), a pulse compression module (104), an imaging module (105), an optical signal collection component (106), an ultrasonic signal collection component (107) and a data processing component (108). Multiphoton imaging and photoacoustic imaging are performed simultaneously by means of the femtosecond pulse laser light source (101), and thus multi-dimensional real-time observation is implemented, and system integration is realized with a low cost.

The invention relates to a multi-modal imaging system (2) for non-invasive examination of an examination object (10), comprising a multi-photon imaging system for providing high-resolution detailed images of the examination object (10), which imaging system comprises a radiation source (12), the latter generating an excitation beam (21) of near infrared femtosecond laser radiation for triggering secondary radiation emitted by the examination object (10), and a focusing optical unit (30), by means of which the radiation of the radiation source (12) is directable at a measurement position of the examination object (10), wherein the focusing optical unit (30) and a laser head (14) of the radiation source (12) are provided in a measuring head (4), which is pivotable, rotatable and flexibly positionable freely in space such that the examination of the examination object (10) is performable under any desired solid angle, and comprising at least one confocal detection device, which is at least partly integrated in the measuring head (4) as well and which is configured to receive a signal of the excitation beam (21) of near infrared femtosecond laser radiation, which was diffusely reflected by the examination object (10).Moreover, a method is specified for non-invasive examination of an examination object (10) using a multi-modal imaging system (2), as is the use of the multi-modal imaging system (2) for examining living matter of the examination object (10)

This invention relates to the field of photon imaging detection, and more particularly to a multi-photon imaging detection system based on trianode photon counting. The system includes a sealed tube imaging detector and a position readout circuit. The sealed tube imaging detector comprises an entrance window coated with a photocathode, a microchannel plate stack, and three anodes. The cross-shaped anode in the trianodes works in conjunction with a multi-channel preamplifier and shaping circuit, a multi-channel analog-to-digital converter circuit, and a microprocessor in the position readout circuit to detect the incident position of photons. The array anode in the trianodes works in conjunction with a multi-channel amplification discriminator circuit and a time measurement circuit in the position readout circuit to detect the arrival time of photons. A synchronization control circuit is used to synchronize the clocks of all circuits, achieving matching between the incident position and arrival time of photons. This invention separates the position measurement and time measurement functions through physical structure, achieving high temporal resolution while retaining high spatial resolution, and solving the problem of position ambiguity caused by multiple synchronously incident photons.

The invention relates to a multi-photon imaging synchronous multi-region data acquisition method and a multi-photon imaging system, and the method comprises the steps: building a multi-photon imaging system through employing a two-photon laser and a three-photon laser with set parameters, and carrying out the focusing depth adjustment of two photons and three photons; the synchronous signal generated by the three-photon laser is subjected to phase reversal processing, and then an acousto-optic modulator of the synchronous two-photon laser is activated to alternately output exciting light; and after frequency multiplication is performed on a synchronizing signal generated by the three-photon laser, the synchronizing signal starts from the acquisition card to drive the photomultiplier, and multi-region two-photon and three-photon imaging signals are synchronously acquired. Compared with the prior art, multi-plane imaging is realized while the imaging quality is ensured, and the risk of light damage to a biological sample is reduced by an alternative excitation strategy.

The application relates to the technical field of microscopic imaging, in particular to a digital adaptive optical method and device for multi-photon imaging, wherein the method comprises the following steps: based on a two-photon synthetic aperture microscope, three-dimensional sample projections are collected by using a small-aperture excitation light beam, small-aperture imaging results of different viewing angles are generated, and a lateral shift vector of images of different sub-apertures caused by wavefront distortion phase in an imaging process is calculated, and in the process of obtaining a three-dimensional reconstruction result based on three-dimensional sample information, optical distortion is compensated according to the lateral shift vector. According to the embodiment of the application, the lateral shift vector generated by each small-aperture image is used to compensate for system optical distortion and perform three-dimensional reconstruction of sample information, so that multi-photon imaging with high resolution and high volume imaging speed is realized, the robustness and practicability of the optical system are improved, and the imaging efficiency is higher.

The invention discloses an MOF-based near-infrared light excitation fluorescent probe and a preparation method and application thereof, and relates to the technical field of biomedical materials.The preparation method of the fluorescent probe comprises the following steps that 1, cobalt salt and a ligand are subjected to a coordination reaction, and Co-MOF is obtained; and (2) carrying out surface modification on the Co-MOF by folic acid to obtain Co-MOF coated FA. The fluorescent probe disclosed by the invention has better biocompatibility and lower toxicity, realizes the function integration of multiphoton imaging, targeted delivery and synergistic anti-inflammation, provides a new technical scheme for precise diagnosis and treatment of inflammation, and also expands the application range of an MOF material in the field of biological medicines.

This application provides a large field-of-view multiphoton imaging system, comprising: a first scanning galvanometer for Y-axis scanning of an image; a first optical transmission module including a first telecentric plan scanning lens and a second telecentric plan scanning lens; a second scanning galvanometer for X-axis scanning of an image; a second optical transmission module including a third telecentric plan scanning lens and a telescope, the telescope including a pair of achromatic cemented doublet lenses placed at a specific distance; an aberration compensation module for correcting optical aberrations in the entire optical path; and an objective lens driven by a piezoelectric actuator for Z-axis scanning of the image.

The invention discloses a multi-photon microscopic image automatic segmentation and regional quantitative feature extraction method based on a weak supervision pseudo label and application thereof. The method is based on lung cancer Hamp; and E, performing multi-photon imaging on the dyed paraffin section, and collecting SHG and TPEF multi-channel high-resolution images. A classification network with ResNet101 as a trunk is adopted, and pixel-level multi-class pseudo labels are generated. And inputting the pseudo tag as a supervision signal into a U-Net segmentation network to complete automatic segmentation of tumor, tumor-related interstitial substance, tumor necrosis and lymphocyte multi-category regions. Based on the segmentation result, multiple quantitative algorithms such as skeletonization, principal axis analysis, branch detection, area and density calculation, fractal and curvature analysis and the like are further integrated, and automatic extraction and standardized statistics of multi-dimensional morphology and spatial distribution characteristics of the target area are achieved. According to the method, the pixel-level manual labeling requirement is greatly reduced, the segmentation precision and the analysis automation level are improved, and the method is suitable for tumor microenvironment, matrix research, molecular pathology auxiliary diagnosis and other scenes.

This invention discloses a multimodal imaging system based on a radio frequency programmable GHz femtosecond pulse train. The system includes a femtosecond pulse laser source, a pulse splitting module, a pulse amplification module, a pulse compression module, an imaging module, an optical signal acquisition component, an ultrasonic signal acquisition component, and a data processing component. The single-pulse laser generated by the femtosecond pulse laser source enters the pulse splitting module, and the resulting femtosecond pulse train sequentially enters the pulse amplification module and the pulse compression module for energy amplification and pulse width compression before entering the imaging module. The imaging module simultaneously performs multiphoton imaging and photoacoustic imaging, with the signals entering the optical signal acquisition component and the ultrasonic signal acquisition component, respectively, to form electrical signals. These electrical signals are then processed by the data processing component to generate the imaging results. This invention, based on a single femtosecond laser, simultaneously performs multiphoton imaging and photoacoustic imaging, enabling multi-dimensional real-time observation. Compared to existing technologies, it offers advantages such as system integration and low cost.

Methods, systems and adaptors for multiphoton transscleral imaging and laser-tissue treatment are provided. Aspects of the present invention include methods of transscleral imaging comprising: deploying an excitation source to transmit light energy through non-transparent tissue comprising scleral tissue; and detecting light emitted via multiphoton excitation through scleral tissue and non-transparent tissues of the eye. Aspects of the present invention further include methods of transscleral imaging comprising: transmitting light energy to a structure through scleral tissue; and imaging the structure using light emitted from the structure via multiphoton excitation through scleral tissue. Aspects of the present invention further include methods of treating tissue, such as ocular or periocular tissue, by manipulating an imaged structure, including, for example, providing non-incisional therapy, photo-tissue interactions, multi-photon-mediated damage, such as thermal damage, photo-disruption, photo cross-linking, blood vessel coagulation or ablating tissue.

The present disclosure provides a multiphoton imaging method. The method includes a) immersing a semi-submersible microscope objective in a liquid medium that is at least one of scattering or absorbing; b) directing laser light through the semi-submersible microscope objective and into the liquid medium in an image-wise manner under conditions such that multiphoton absorption by the multiphoton absorber occurs, and at least partial polymerization of the polymerizable compound occurs resulting in an article; and c) removing uncured polymerizable compound to clean the article. The liquid medium includes a polymerizable compound, a secondary component, and a multiphoton absorber. An article is also provided. The article includes a material defining one or more tortuous or arcuate channels, one or more internal architectural voids, one or more undercuts, one or more perforations, or combinations thereof, at least one of which exhibits a surface roughness of 1.0 micrometer Ra or less.

Probe tip structures are disclosed that can replaccably couple to an imaging probe to provide a sterile interface between an imaging probe and the tissue undergoing analysis. The probe and associated tip apparatus can then be used for noninvasive or minimally invasive in vivo microscopy to visualize the surface or lining of internal organs and / or tissue. The tip apparatus can be attached to the probe before (or during the procedure) and then removed and discarded, such that the probe, itself, remains relatively free of contamination and more readily sterilizable for reuse. Alternatively, the tip apparatus can be sterilizable and reusable. The tip structures are particularly useful in conjunction with multi-photon imaging probes.

The embodiment of the present application provides a kind of acquisition method of multi-photon imaging virtual simulation model and three-dimensional image generation method, it is related to multi-photon imaging technical field.The acquisition method of multi-photon imaging virtual simulation model includes: constructing the training set of multiple sample data including target tissue, each sample data includes: the original dyeing image of target tissue matched with the real three-dimensional image obtained by multi-photon imaging;Multi-photon imaging virtual simulation model based on GAN network and self-attention mechanism is constructed, the input of the multi-photon imaging virtual simulation model is the dyeing image of target tissue, and the output is the virtual three-dimensional image of target tissue;The multiple sample data are used to train the multi-photon imaging virtual simulation model, and the final multi-photon imaging virtual simulation model is obtained.The present application realizes the conversion from dyeing image to virtual three-dimensional image, and conversion precision is higher.