Multi-path, multi-magnification, non-confocal fluorescence emission endoscopy apparatus and methods

Abstract

Embodiments of the invention include an optical system and an optical system module, coupled to a distal end of a fluorescence emission endoscope apparatus, an optical waveguide-based fluorescence emission endoscopy system, and a method for remotely-controlled, multi-magnification imaging of a target or fluorescence emission collection from a target with a fluorescence emission endoscope apparatus. An exemplary system includes an objective lens disposed in a distal end of an endoscope apparatus. The lens is adapted to transmit both a visible target illumination and a fluorescence-emission-inducing target illumination as well as fluorescence-emission and visible light from the target. The system can thus simultaneously provide low magnification, large field of view imaging and high magnification, high-resolution multiphoton imaging with a single lens system.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority to U.S. Provisional application Ser. No. 60 / 987,868 filed on Nov. 14, 2007, and to U.S. Provisional application Ser. No. 60 / 987,270 filed on Nov. 12, 2007, the subject matters of which are incorporated by reference herein in their entireties.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT[0002]This invention was made with government support under Grant No. 1R01EB006736-01 and Grant No. 5-P41EB001976 sponsored by the National Institute of Biological Imaging and Bioengineering at the National Institutes of Health. The government has certain rights in the invention.BACKGROUND OF THE INVENTION[0003]1) Field of the Invention[0004]Embodiments of the invention are most generally related to the field of multiphoton fluorescence and / or non-linear harmonic emission endoscopy apparatus and methods. More particularly, embodiments of the invention are directed to multi-magnification, non-confocal, multi...

AI Technical Summary

Benefits of technology

The patent text describes an invention that aims to improve the performance of endoscopy imaging apparatus and methods by incorporating the principles of multiphoton fluorescence and non-linear optical phenomena. The invention addresses challenges and shortcomings associated with current technology, such as limited imaging depth, high energy light damage, and inefficient signal collection and transmission. The technical effects of the invention include improved image quality, reduced pain and time associated with biopsy procedures, and improved sub-tissue, nerve, and cartilage examination. The invention also addresses the need for apparatus and methods that can provide both low magnification, large field of view imaging and high magnification, high-resolution multiphoton imaging in a practical, cost-effective, and efficacious manner.

Problems solved by technology

The use of high energy light could easily damage living tissue in the entire region of exposure.

Although multi-optical systems are routinely provided in microscopy apparatus, separate, switchable optical systems do not provide a suitable architecture for a compact endoscope.

The inventors have also recognized that conventional confocal-based endoscopy imaging apparatus and methods can be disadvantageous due, for example, to image obstruction from scanner apparatus and inefficient signal collection and / or transmission via a concentric excitation fiber waveguide.

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