The invention relates to a test strip used for detecting immune colloidal gold remaining in kanamycin. The test strip comprises a sample pad, a combination pad, a cellulose nitrate membrane, a water absorbing pad and a PVC back lining, wherein, the sample pad, the combination pad, the cellulose nitrate membrane and the water absorbing pad are continuously stuck on the PVC back lining in sequence,lapping is arranged between the combination pad and the cellulose nitrate membrane, and lapping is arranged between the cellulose nitrate membrane and the water absorbing pad; an anti-kanamycin monoclonal antibody-colloidal gold label is coated on the combination pad, and a detection line formed by a kanamycin-carrier protein (bovine serum albumin) coupling compound and a quality controlling lineformed by a rabbit anti mouse are coated on the cellulose nitrate membrane. The test strip of the invention has the advantages that the specificity is strong, the sensitivity is high, the detection time is short (10 minutes), the test strip can be operated on site, the detection cost is low, the operation is simple, and the test strip is not required to be operated by professionals.