Liquid phase chip for joint detection of multiple tumor markers and preparation method thereof

Abstract

The invention relates to a liquid phase chip for joint detection of multiple tumor markers and a preparation method thereof. The liquid phase chip comprises micro-balloons of a coupled antibody (at least two of the followings: AFP, CEA, CA125, CA153, CA19-9, CA242, CA72-4, PSA, HGH, Beta-HCG), corresponding biotin-labeled detection antibodies, streptavidin phycoerythrin and vegetable hydrosol or polysaccharide hydrosol which has a solute content of 1-10 wt per thousand and does not contain protein. The preparation of the liquid phase chip comprises refining and purification of hydrosol, coupling between captured antibodies and micro-balloons, preparation of biotin-labeled antibodies, dispersing the coupled micro-balloons into the vegetable hydrosol or the polysaccharide hydrosol, and the like. The liquid phase chip has the advantages of stable performance, good micro-balloon dispersivity, long preservation time, fast and convenient use and operation, small amount of samples in use, high detection sensitivity, wide linear scope and low detection cost, can detect ten tumor markers at most at one time, and requires a cost which is a quarter of the total fee of conventional methods.

Description

technical field

[0001] The invention relates to the field of biochips, in particular to a liquid phase chip for combined detection of multiple tumor markers and a preparation method thereof. Background technique

[0002] Tumor markers refer to a large class of substances produced by tumor tissue that exist in the tumor tissue itself or are secreted into blood and other body fluids, or are produced by host cells due to tumor cell stimulation and have significantly abnormal levels. Tumor markers play an important role in the clinical diagnosis and treatment of tumors. The detection of the presence or content of tumor markers in blood or body fluids can not only be used for general screening, early diagnosis, auxiliary diagnosis, benign and malignant differentiation, and clinical staging of tumors. At the same time, it is also of great value in monitoring curative effect, judging prognosis, and predicting tumor recurrence and metastasis. The most commonly used clinical tumor m...

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