23 results about "Phycoerythrin" patented technology

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in macrophage polarization and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture system is used for preparing a capture antibody coupled with a fluorescence coding microsphere by coupling a biotinylated antibody with a fluorescence microsphere coated with biotin, and the detection system is used for preparing a fluorescence labeled antibody by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody. The mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect multiple cell factors, the sample dosage is only 25 microliters, the kit is suitable for in-vitro tests of mouse mononuclear macrophage leukemia cell lines RAW264.7, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention belongs to the technical field of haematococcus separation, and particularly relates to a method for sequentially separating polysaccharide and phycoerythrin from a haematococcus degreased algae residue water extract, and the method comprises the following steps: S1, pretreatment, S2, deep flocculation and primary separation of polysaccharide, and S3, specific precipitation of phycoerythrin. According to the method, a set of sequential separation process is constructed by adopting a self-made hydrophobic flocculant and a composite precipitation inducer, so that a high-purity polysaccharide product and a high-activity phycoerythrin concentrated solution can be synchronously obtained from a haematococcus degreased algae residue water extract. According to the method, deep flocculation of polysaccharide, specific precipitation of phycoerythrin and respective subsequent refining steps are integrated into an ordered whole process, so that the product in the previous step can be directly treated in the next step in an optimal state, and the loss possibly caused by storage and transfer of intermediate materials is reduced; through condition matching and cooperation among the steps, the purity of the final product is improved.

Disclosed herein is a method for enhancing immune response of a vaccine. The method includes preparing a Phycocyanin compound. The Phycocyanin compound includes at least one of C-phycocyanin (C-PC), Phycoerythrin (PE), Allophycocyanin (APC), phycocyanobilin (PCB), and combinations thereof. The method further includes administering the prepared Phycocyanin along with a vaccine composition to a living body.

The invention discloses a multi-drug-resistance gene detection system and method based on a suspension array technology and application. The multi-drug-resistance gene detection system comprises coding microspheres, a primer group, a fluorescence labeled anti-Tag ssDNA molecule and phycoerythrin. The invention constructs a detection method aiming at a plurality of bacteria and drug-resistant genes by a suspension array technology, designs a plurality of pairs of specific primers of bacteria and drug-resistant genes with Tag labels, captures Tag labeled products after multiple PCR amplification by anti-Tag fixed on a suspension array, realizes accurate detection, and has the advantages of high specificity, high sensitivity, high sensitivity and high sensitivity. According to the present invention, with the kit, the four types of bacteria (E. coli, A. brumanni, K. pneumonae and P. aeruginosa) and the eight types of drug-resistant genes (blaKPC-2-like, blaNDM-like, blaOXA-48-like, blaOXA-23-like, blaIMP-like, blaVIM-like, blaCMY-2-like and blaADC-68-like) can be simultaneously detected in the same reaction system, such that the reagent consumption is reduced, the cost is reduced, and the detection time is shortened.

The invention provides an anti-CD4 antibody as well as a microsphere flow immunofluorescence luminescence detection kit and application thereof, and provides two monoclonal antibodies for recognizing different binding sites of a CD4 antigen, an antibody coupling system based on magnetic fluorescent microspheres, a biotinylation detection antibody and phycoerythrin labeled streptavidin. The invention also discloses a corresponding detection method and a preparation method of the kit. The CD4 antigen detection kit and the antibody combination thereof provided by the invention can significantly improve the sensitivity, stability, specificity and flux capacity of CD4 detection, and are suitable for multiple fields of immune function evaluation, autoimmune disease evaluation, inflammatory disease research, HIV infection auxiliary diagnosis and the like.

The invention provides an immune detection method of an immune checkpoint detection kit, and belongs to the technical field of immune checkpoint detection kits.The immune detection method comprises the steps that an immune reaction system containing immune checkpoint antibodies is prepared, incubation is conducted under the constant temperature condition to form a sandwich type immune complex, and the sandwich type immune complex is obtained; phycoerythrin labeled streptavidin is added for fluorescence labeling, a flow cytometer is adopted for fluorescence signal detection after interference substances are removed through multiple times of cleaning, fluorescence image data are processed through an image partitioning optimization model, image processing parameters are dynamically adjusted through a signal enhancement algorithm function and a gating weight function, and the fluorescence image is obtained. A multi-scale feature extraction network based on a visual converter architecture is adopted to realize accurate processing of a complex fluorescence image, finally correlation analysis and matrix decomposition calculation are performed on fluorescence intensity data, and a detection analysis model is established to output index concentration values of immune checkpoints. The problem that complex fluorescence image data are difficult to process accurately at present is solved.

The invention provides an anti-ADGRG1 antibody as well as a microsphere flow type immunofluorescence luminescence detection kit and application thereof, and provides two monoclonal antibodies for recognizing different binding sites of an ADGRG1 antigen, an antibody coupling system based on magnetic fluorescent microspheres, a biotinylation detection antibody and phycoerythrin labeled streptavidin. The invention also discloses a corresponding detection method and a preparation method of the kit. The ADGRG1 antigen detection kit and the antibody combination thereof provided by the invention can significantly improve the sensitivity, stability, specificity and flux capacity of ADGRG1 detection, and are suitable for multiple fields of immune function evaluation, autoimmune disease evaluation, inflammatory disease research, HIV infection auxiliary diagnosis and the like.

The present application belongs to the technical field of biological detection, and provides a kit for detecting cytokines in blood samples. The kit comprises fluorescent coded polystyrene magnetic microspheres coupled with capture antibodies, biotin-labeled paired detection antibodies, streptavidin-labeled phycoerythrin with a final concentration of 0.5-2 ug / mL, a washing buffer containing 0.05%-0.1% Tween-20 or triton-100, an experimental buffer containing 0.5%-2% bovine serum albumin, and a sample diluent. The sample diluent comprises the experimental buffer and treated human serum. The treated human serum is obtained by first heat inactivation and carbon adsorption treatment of human serum, and then centrifugal separation using an ultrafiltration centrifugal tube. By using the kit provided by the present application, quantitative results of 7 cytokines can be obtained in a single detection of a single sample, and the detection range can reach 0.977-128000 pg / mL.

The present disclosure describes systems and methods for extracting one or more compounds from aquatic plants, such as, seaweed, although other plants or plant types are possible. As described in more detail below, seaweed and certain other plants may contain useful compounds and materials, such as bromoform (or some other halogenated compound) and / or phycoerythrin (PE). The useful compounds may be extracted into a particular phase (e.g., an aqueous phase, an organic phase) and, in some cases, further purified. In some cases, a majority or an entirety of the seaweed can be used. That is, in some embodiments, useful compounds are extracted from the seaweed and the remaining seaweed itself (i.e., the seaweed remaining after the useful compounds have been extracted) may also be useful in that it may contain useful nutrients (e.g., protein) that can be used as a feedstock (e.g., an animal feedstock) and / or may be used as an additive to existing feedstock.

The invention provides an immune checkpoint joint detection method based on a multi-microsphere flow-type immunofluorescence method, and belongs to the technical field of multi-microsphere flow-type immunofluorescence method detection.The immune checkpoint joint detection method comprises the steps that seven fluorescent microspheres are coupled with PD-L2, TIM-3, TNFRSF7, PD-1, PD-L1, LAG-3 and CTLA-4 to capture antibodies to form a multi-detection system; the method comprises the following steps: incubating a plasma sample and an antibody microsphere mixed solution to form a first immune complex, adding a biotin-labeled detection antibody and phycoerythrin-labeled streptavidin to construct a fluorescence-labeled sandwich immune complex, detecting by using a flow cytometry to obtain fluorescence intensity data, and converting the fluorescence intensity data into a concentration value; and the optimal immune checkpoint combination is screened by applying a multi-dimensional Carnot diagram optimization algorithm, and a combination modulation factor is calculated to determine the disease risk level, so that the technical problem that the accuracy of disease risk assessment is insufficient due to the fact that multi-index collaborative analysis cannot be realized in immune checkpoint molecular detection is solved.

The invention relates to an Alzheimer's disease blood marker flow type fluorescence luminescence multi-joint detection kit and application thereof. The Alzheimer's disease blood marker flow type fluorescence luminescence multi-joint detection kit comprises a capture microsphere mixed solution, a detection antibody mixed solution, a streptavidin-phycoerythrin conjugate, an interference elimination solution and a washing solution. The bihexanamide-biotin is labeled on the detection antibody, the steric hindrance of combination of the streptavidin-phycoerythrin conjugate and the biotin on the detection antibody is reduced through bihexanamide, more phycoerythrin can be combined on a single antibody, a fluorescence signal is remarkably enhanced, and the detection sensitivity is greatly improved. An interference elimination solution containing dimethyl sulfoxide and Proclin300 at the same time is adopted and mixed with a plasma sample, then a capture antibody and a detection antibody are added, non-specific adsorption of lipids and binding proteins thereof in plasma on the surfaces of microspheres is weakened, and the detection accuracy is greatly improved. According to the method, a machine learning model based on a logistic regression algorithm is constructed through a joint detection result of seven markers in three layers, and the judgment accuracy is cooperatively improved.

The application discloses a phycobiliprotein PE-1 with fluorescence characteristics, a fusion protein thereof and application, and belongs to the technical field of genetic engineering. The application provides a novel phycobiliprotein, and the amino acid sequence of the phycobiliprotein is shown as SEQ ID NO. 1. The phycobiliprotein has high fluorescence quantum yield and fluorescence emission capacity. The application further provides a fusion protein constructed by fusing the phycobiliprotein and streptavidin through a connecting peptide, a recombinant expression vector containing the coding sequence of the fusion protein and a recombinant host cell capable of expressing the fusion protein. The fusion protein provided by the application can be used in solid-phase immunodetection systems such as ELISA and immunochromatography, and sensitive detection of target objects can be realized through a fluorescence detection mode of 540 / 580 nm. The fusion protein provided by the application has significant advantages in signal-to-noise ratio and detection stability, and can be widely applied to the fields of biomedical detection and diagnosis.

The application discloses an immune cell autophagy marker detection kit and belongs to the technical field of biomedical detection, comprising two kinds of fluorescent coding microsphere mixtures coupled with anti-LC3B-II and p62 / SQSTM1 capture antibodies respectively, a biotinylated detection antibody mixture, a streptavidin-phycoerythrin conjugate, six-point calibrators (S0-S5), a quality control product, a cell lysis buffer containing non-ionic and zwitterionic surfactants and 4 mol / L urea, and data analysis software based on LC3B-II / p62 natural logarithm ratio output autophagy activity comprehensive score.

The invention belongs to the technical field of biological detection, and provides a chemokine detection kit and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture system is used for preparing a capture antibody coupled with a fluorescence coding microsphere by coupling a biotinylated antibody with a fluorescence microsphere coated with biotin, and the detection system is used for preparing a fluorescence labeled antibody by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody. The mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cytokines, the sample dosage is only 25 microliters, the kit is suitable for detecting chemotactic factors in a mouse in-vitro bone marrow-derived macrophage (BMDM) polarization model, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of high flux, wide linear range and high stability.

Methods and systems for extracting phycobiliproteins from seaweed, such as phycoerythrin, are generally described. In some embodiments, the phycobiliprotein extracted from seaweed is phycoerythrin, and the methods and systems described by this disclosure may extract phycoerythrin from seaweed while maintaining other compounds within the seaweed, such as bromoform.

The application discloses a PE / fluorescent tandem dye, a preparation method thereof and a method for labeling an antibody, and the free thiol group on phycoerythrin is blocked; then small-molecule organic dye tandem treatment is carried out; then protein protection treatment is carried out on the obtained tandem dye; finally, activation treatment is carried out by using a crosslinking agent; and a PE / fluorescent tandem dye is obtained. When the antibody is labeled, firstly, thiolation treatment is carried out on the target antibody by using a reducing agent; then the thiolated target antibody is crosslinked with the activated tandem dye; then a blocking agent is added into the crosslinked tandem dye-antibody system for dye blocking treatment, and the fluorescent antibody is preserved, and the antibody labeling is completed. The PE and the small-molecule organic dye are combined by a covalent bond, the donor PE is excited by laser emitted by an exciter, the energy emitted after excitation is transferred to the small-molecule organic dye, the small-molecule organic dye is excited to emit light of a specific wavelength, and the PE / fluorescent tandem dye is a fluorescent dye with a unique fluorophore.

The invention discloses phycoerythrin PE-1 with fluorescence characteristics and a fusion protein and application thereof, and belongs to the technical field of gene engineering. The invention provides novel phycoerythrin. The amino acid sequence of the phycoerythrin is shown as SEQ ID NO. 1. The phycoerythrin has high fluorescence quantum yield and fluorescence emission capability. The invention also provides a fusion protein constructed by fusion of phycoerythrin and streptavidin through a connecting peptide, a recombinant expression vector containing a coding sequence of the fusion protein, and a recombinant host cell capable of expressing the fusion protein. The fusion protein provided by the invention can be used for solid-phase immunodetection systems such as ELISA (Enzyme-Linked Immunosorbent Assay), immunochromatography and the like, and sensitive detection on a target object is realized through a 540 / 580nm fluorescence detection mode. The fusion protein provided by the invention has remarkable advantages in the aspects of signal-to-noise ratio and detection stability, and can be widely applied to the field of biomedical detection and diagnosis.

This invention discloses a large-scale preparation method for high-purity phycobiliproteins, belonging to the field of purification technology for active ingredients in natural products. This invention utilizes the addition of electronegative polysaccharides or oligosaccharides to form a co-soluble solution, which induces phycoerythrin precipitation under low-temperature conditions, achieving effective separation of phycoerythrin and phycocyanin and obtaining high-purity phycoerythrin. This invention, through variable-parameter aqueous two-phase continuous purification, obtains high-purity phycocyanin while effectively removing both existing and added soluble polysaccharides from the phycobiliprotein-containing solution or biomass extract, avoiding interference from the large molecular size and negative charge of polysaccharides on ultrafiltration purification performance. The prepared phycoerythrin has a PE / PC molar ratio >14, and the phycocyanin has a PC / PE molar ratio >4. Compared with traditional purification methods, this invention effectively solves the high cost and complexity of chromatographic preparation of phycobiliproteins. The method for preparing and purifying phycobiliproteins provided by this invention has the advantages of fewer purification steps, high yield, simple process, and easy scalability. Furthermore, the preparation process is green, environmentally friendly, and low-cost, facilitating industrial production and meeting market demand for high-purity phycobiliproteins, thus possessing significant promotional value.

The invention provides a multi-microsphere flow type fluorescence detection method for vascular inflammation markers, and belongs to the technical field of multi-microsphere flow type fluorescence detection.The multi-microsphere flow type fluorescence detection method comprises the steps that capture antibody coupling microsphere mixed liquor with different fluorescence coding characteristics is prepared; the method comprises the following steps: sequentially reacting with a to-be-detected sample, a biotin labeled detection antibody and phycoerythrin labeled streptavidin to form a sandwich immune complex, collecting a fluorescence signal by using a flow cytometry, and then carrying out self-adaptive optimization treatment on the original fluorescence signal by using a liquid dynamic correction model, according to the dynamic response characteristics of liquid neurons in the liquid reservoir layer, accurate concentration results of the five vascular inflammation markers are calculated by fitting a standard curve through a logistic regression model, and the technical problem that the multiple detection precision of the vascular inflammation markers is insufficient due to limited dynamic range of fluorescence signals and serious background noise interference is solved.

This invention belongs to the field of Haematococcus pluvialis separation technology, specifically relating to a method for sequentially separating polysaccharides and phycoerythrin from the aqueous extract of degreased Haematococcus pluvialis residue. The method includes the following steps: S1. Pretreatment; S2. Deep flocculation and primary separation of polysaccharides; and S3. Specific precipitation of phycoerythrin. This invention utilizes a self-made hydrophobic flocculant and a composite precipitation inducer to construct a sequential separation process, thereby enabling the simultaneous acquisition of high-purity polysaccharide products and highly active phycoerythrin concentrate from the aqueous extract of degreased Haematococcus pluvialis residue. This invention integrates the deep flocculation of polysaccharides, the specific precipitation of phycoerythrin, and their respective subsequent purification steps into an orderly overall process, ensuring that the output of the previous step can directly enter the next processing step in the most suitable state. This not only reduces potential losses from the storage and transfer of intermediate materials but also improves the purity of the final product through condition matching and synergy between steps.

The present application relates to a kind of anti-fake viscose composite sewing thread and preparation method thereof, belong to sewing thread technical field.The present application is first to the cationization pretreatment of viscose fiber to improve its binding force with anionic dye, then four different dyeing viscose fibers are prepared using natural fluorescent dye turmeric, madder and protein fluorescent dye phycocyanin, phycoerythrin respectively;Natural dyeing fiber is designed as relatively thick fineness and relatively long cut length, protein fluorescent dyeing fiber is designed as relatively thin fineness and relatively short cut length, mixed into base fiber according to equal proportion, is spun into anti-fake viscose composite sewing thread by clearing and combing, drawing, roving, spinning, winding and doubling twisting process, the sewing thread can present corresponding characteristic fluorescent color under specific wavelength excitation, and fluorescent hue and fiber fineness, cut length form fixed matching relationship, thus construct " fluorescent characteristic + fineness + length " multiscale composite anti-fake system, form difficult to imitate anti-fake password.