248 results about "Biotin" patented technology

The invention relates to the technical field of exosomes, in particular to an exosome compound preparation with a hair growing effect and a preparation method of the exosome compound preparation. Comprising 12 to 22 parts of ginsenoside Rb1, 15 to 12 parts of biotin tripeptide-15, 8 to 15 parts of diammonium glycyrrhizinate, 50 to 70 parts of fish collagen peptide, 10 to 20 parts of soybean peptide, 0.5 to 4.5 parts of SHH protein, 0.5 to 2.5 parts of SMO inhibitor, 0.5 to 1.5 parts of HhSP protein, 5 to 10 parts of N-acetyl tyrosine, 0.1 to 2 parts of hyaluronic acid, 0.1 to 1 part of melatonin, 0.1 to 1 part of quercetin, 0.1 to 1 part of kaempferol, 0.1 to 1 part of tanshinone, 0.1 to 1 part of biotin and 0.1 to 1 part of vitamin A. The compound preparation is combined with exosomes from various sources, so that the hair papilla cell function is synergistically enhanced, and the damaged hair follicle structure is repaired.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in macrophage polarization and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture system is used for preparing a capture antibody coupled with a fluorescence coding microsphere by coupling a biotinylated antibody with a fluorescence microsphere coated with biotin, and the detection system is used for preparing a fluorescence labeled antibody by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody. The mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect multiple cell factors, the sample dosage is only 25 microliters, the kit is suitable for in-vitro tests of mouse mononuclear macrophage leukemia cell lines RAW264.7, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention relates to the technical field of bacterial detection, in particular to a nucleic acid colorimetric / photo-thermal dual-mode instant detection kit and system based on CRISPR / Cas12a and Au (at) PtNPs. One purpose of the invention is to provide a nucleic acid colorimetric / photo-thermal dual-mode instant detection kit based on CRISPR / Cas12a and Au (at) PtNPs. The nucleic acid colorimetric / photo-thermal dual-mode instant detection kit based on CRISPR / Cas12a and Au (at) PtNPs comprises the following components: Au (at) PtNPs coupled with Biotin-ssDNA-SH, a specific primer targeting a GBS conserved gene, a CRISPR / Cas12a cleavage reaction mixed solution and a reaction mixed solution. According to the technical scheme, the high robustness and reliability of the detection result in bedside, community clinics and other basic-level application scenes are jointly ensured through the simplified pretreatment process and the internal cross validation mechanism of the colorimetric / photo-thermal dual-mode signals, and a solid foundation is laid for wide popularization of the technology.

The application discloses a covalent polypeptide inhibitor targeting protein kinase A, a conjugate, a kit, a pharmaceutical composition and application. The general formula of the covalent polypeptide inhibitor is Z1-Seq-Z2; wherein Seq is an amino acid sequence; the C-terminal of Seq comprises a pseudo-substrate recognition motif recognized by protein kinase A, a non-natural amino acid with a covalent reaction group, and can be covalently crosslinked with a Cys200 residue on a catalytic subunit of protein kinase A; Z1 is selected from hydrogen, an acyl group, a fluorescent group, biotin, an isotopic tag or a biological ortho-reaction group; and Z2 is selected from a hydroxyl group, an amino group, a cell-penetrating peptide or a stabilization modification group. The covalent polypeptide inhibitor can strongly and irreversibly inhibit PKA activity, and can be used as an active probe to specifically label active PKA in a complex biological sample, and is used for biological research and drug development.

The present invention relates to a composition, a kit and a detection method for detecting hydrogen peroxide on the basis of self-biotinylation according to the peroxidation activity of a guanine quadruplex, and, more specifically, to a composition, a kit and a detection method for detecting hydrogen peroxide, the composition comprising: i) a guanine quadruplex-hemin complex (G-quadruplex-hemin complex); ii) biotinyl tyramide; and iii) a probe capable of binding to the guanine quadruplex.

The application discloses a fucose glycoprotein detection kit, a preparation method thereof and a fucose glycoprotein color developing method. First, a liquid sample is dropped on the surface of the kit, and the sample and test paper are infiltrated through a sample inlet. Under the action of transverse flow force, the sample flows to an observation window along a hydrophilic test paper. The glycoprotein containing alpha 1,2-fucose in the sample is combined with agglutinin 1, and the rest of the sample continues to flow to the next layer of test paper. Then, the glycoprotein containing alpha 1,3-fucose is combined with agglutinin 2, and the rest of the sample flows to the third layer and is combined with agglutinin 3 containing alpha 1,6-fucose glycoprotein. All the combined fucose glycoproteins are combined with agglutinin biotin through their antibodies, and horseradish peroxidase is used for labeling color development, so that quantitative detection of the fucose glycoprotein in the sample is realized. The method has important significance for studying the changes of different connection fucose glycoproteins in cells and body fluids in diseases.

This invention discloses a Zn-based framework material, Bi-Cur@Zn-MOF, its preparation method, and its applications in antibacterial and antioxidant properties, belonging to the field of functional materials and biomedical technology. The method specifically involves: dissolving zinc salt in water to obtain a zinc salt solution; dissolving curcumin and biotin together in water to obtain a mixed solution, and adjusting the pH of the mixed solution; mixing and stirring the zinc salt solution and the mixed solution evenly to obtain a precursor solution; subjecting the precursor solution to a hydrothermal reaction; after the reaction, performing solid-liquid separation and collecting the solid; washing and vacuum drying the solid to obtain the Zn-based framework material, Bi-Cur@Zn-MOF. The preparation method of this invention is green and simple, requiring no toxic reagents, and is suitable for large-scale preparation. The Bi-Cur@Zn-MOF prepared by this invention possesses both antibacterial and antioxidant functions, exhibits structural stability and good biocompatibility, and can be used to prepare medical dressings, food packaging materials, skin care products, wound repair, and antioxidant and antibacterial coatings, etc.

The present disclosure discloses a composition for hair growth comprising Larix europea wood extract, Camellia sinesis leaf extract, Pisum sativum sprout extract, Curcuma longa (Turmeric) callus conditioned media, Hamamelis virginiana (Witch Hazel), Rosmarinus officinalis Leaf Oil, Glycerin, Glycine, Zinc Chloride, Sodium Metabisulfite, Gluconolactone, Sodium Benzoate, Calcium Gluconate, Tetrasodium ethylenediaminetetraacetic acid (EDTA), Saccharide Isomerate, Hyaluronic Acid, Hydrolyzed Hyaluronic Acid, Propanediol, Biotinoyl Tripeptide- 1, Alpha Glucosyl Hesperidin, Caffeine, Hydrolyzed Pea Protein, Polyalkylene Glycol Mono Butyl Ether, Acrylates / C 10-30 Alkyl Acrylate Crosspolymer, Panthenol, Niacinamide, Arginine, PEG-40 Hydrogenated Castor Oil, Ethylhexylglycerin, Phenoxyethanol, and water is added to the composition until the desired volume is achieved. A method for preparing the composition for hair growth is also provided.

A full continuous-flow preparation method of (+)-biotin, including: subjecting a cyclic anhydride and a chiral biphenyl propylene glycol to asymmetric ring-opening reaction to produce a first intermediate, which undergoes selective reduction with a borohydride and cyclization with an inorganic mineral acid to produce (3aS, 6aR)-lactone; subjecting the (3aS, 6aR)-lactone and a sulfenylating reagent to sulfenylation to produce (3aS, 6aR)-thiolactone, which undergoes Fukuyama coupling with a zinc reagent in the presence of a palladium catalyst and elimination reaction in the presence of an inorganic mineral acid to produce an alkenyl valerate compound; subjecting the alkenyl valerate compound to reduction in the presence of a Pd / C catalyst to produce a valerate ester, which undergoes hydrolysis to produce a valeric acid salt; and subjecting the valeric acid salt to debenzylation in the presence of an inorganic mineral acid to produce the target product (+)-biotin.

The application provides a method for detecting the labeling efficiency of protein biotin, characterized in that: after denaturation of the protein, the protein is incubated with blocked magnetic beads, and the labeling efficiency of the biotin protein is calculated through the concentration change of the biotin protein before and after incubation with the magnetic beads. The scheme has good stability and high accuracy.

The application discloses a kind of cell membrane chromatographic column based on biotin ligase directional covalent fixation and its preparation method and application, belong to chromatography science and technology field. By bonding 2-imino biotin to silica gel, then using biotin ligase specific recognition with Avi-Tag cell membrane fragments, and stable covalent bonding occurs with 2-imino biotin silica gel, so that cell membrane is fixed on the surface of silica gel to obtain Avi-Tag bridged cell membrane chromatographic stationary phase, finally using wet column packing, obtain cell membrane chromatographic column based on biotin ligase directional covalent fixation. The cell membrane chromatographic column can prolong the service life of cell membrane chromatographic column, improve the stability of cell membrane chromatographic column during use, and the short peptide tag (Avi-Tag) used can improve the non-specific adsorption and poor specificity of the cell membrane chromatographic column prepared based on protein tag technology to a certain extent, providing technical support for the wide application of cell membrane chromatographic column.

The invention belongs to the technical field of proteomics detection, and particularly relates to a method for detecting mouse liver neomembrane proteome based on an orthogonal translation system and a mouse strain containing an SORT-KASM module. Comprising the following steps: (S.1) constructing a conditionally expressed SORT-KASM transgenic mouse strain; (S.2) activating the expression of the SORT-KAS M by a tissue specific Cre system; (S.3) giving unnatural amino acid ingestion to the mouse; and (S.4) carrying out biotin labeling coupling on non-natural amino acids in the proteome through an azide-alkyne click reaction. According to the detection method, the space and time high resolution of the liver tissue is achieved, the membrane protein has the high-coverage marking capacity, the low-abundance newborn protein has the high-sensitivity marking capacity, good stability is achieved, and the membrane protein participates in the important physiological processes such as substance transfer, energy metabolism and steady state maintaining; the innovation of the detection method provides a new thought for researching the liver cell membrane proteome.

The invention discloses a method for efficiently preparing fixed-point biotinylation recombinant protein in escherichia coli, and belongs to the technical field of biology. According to the invention, a weakened expression regulatory sequence regulates and encodes a biotin ligase (BirA) gene expression cassette and a target protein gene expression cassette encoding a sequence carrying a biotin receptor peptide tag (AVI-Tag) to be jointly constructed on an escherichia coli expression vector, so that efficient fixed-point biotinylation of a target protein in an escherichia coli body is realized. The method has the advantages of high biotinylation efficiency, high specificity, simplicity and convenience in operation and the like, can be widely applied to the fields of separation and purification of proteins, protein interaction research and the like, and has important practical application value.

The present invention relates to a biologically active material conjugate in which a biological active material is conjugated with a biotin moiety, a fatty acid moiety, or a combination thereof and, more specifically, to a biologically active material conjugate having a biotin moiety and a fatty acid moiety coupled thereto. With a biotin moiety and a fatty acid moiety coupled thereto, the biologically active material according to the present invention exhibits an excellent in-vivo oral absorption rate and has an excellent pharmacokinetic effect.

It is an object of the present invention to provide a compound that has absorption in a near infrared region, has high efficiency of singlet oxygen generation (quantum yield), and also has high tumor toxicity when it is combined with an immunotoxin. According to the present invention, provided is a compound represented by the following formula (1) or a salt thereof:wherein L1 and L2 each independently represent a single bond, —O—, —CO—, an alkylene group containing 1 to 8 carbon atoms, a sugar chain, or a combination thereof; R1 and R2 each independently represent an alkyl group containing 1 to 8 carbon atoms, a carboxylic acid group, an amino group, a hydroxyl group, a thiol group, or a biotin residue; R3, R4, R5, R6, R7 and R8 each independently represent an alkyl group containing 1 to 8 carbon atoms, a phenyl group, a carboxylic acid group, an amino group, a hydroxyl group, a thiol group, or a biotin residue; and M represents Mg, Zn, Fe, P, Si, Cu, Sn, Al, Ti, Mo, or Ni.

A method for analyzing related substances in a fequesetide preparation. In the method, high-performance liquid chromatography is used for determination, where the chromatographic column used therein is a YMC-Triart C18 chromatographic column, 4.6 × 150 mm, 3 µm; the column temperature is 35°C; the sample size is 100 mL; the detection wavelength is 210 nm; the mobile phase A is a 0.1% aqueous trifluoroacetic acid; the mobile phase B is 0.1% trifluoroacetic acid-acetonitrile; and gradient elution is used, and the elution program is as follows: at 0 min: the volume ratio of the mobile phase A to the mobile phase B is 100:0; at 8-13 min: the volume ratio of the mobile phase A to the mobile phase B is 85:15; and from 13 min to 20 min: the volume ratio of the mobile phase A to the mobile phase B is 100:0. In the present analysis method, the degree of separation between a main peak and impurity peaks is good, the peak pattern, tailing and number of theoretical plates are good, and the detection time is short; therefore, an effective method is provided for formulating quality standards for products of this type.

The application discloses a kind of quick site analysis method and detection system of biotin labeled KRAS protein, belong to intelligent analysis technical field;Construct the site fluorescence intensity database under historical detection state, obtain the fluorescence intensity data of each amino acid site;Based on the data, construct the amino acid site-fluorescence intensity coordinate system of each sample, generate fluorescence intensity fluctuation curve;Extract the wave crest and wave trough of curve, construct fluorescence peak site pair set and valley site pair set;Obtain the mode of peak site pair and valley site pair in all samples, as high response site and low response site, calculate its probability of occurrence in new sample, and combine the peak value and valley value of real-time sample with preset threshold, output high response type, low response type or stable type classification result.The application realizes the quick, objective, intelligent discrimination of KRAS protein function state by historical data probabilistic modeling and fluctuation curve feature extraction.

The invention discloses preparation and application of algal polysaccharide-based co-drug-loading tumor targeting nanoparticles. The algal polysaccharide-based co-drug-loaded nano particle disclosed by the invention is a self-assembled targeting nano particle co-constructed by biotin modified algal polysaccharide sulfate and arginine modified chitosan. The algal polysaccharide-based co-drug-loaded nano particle disclosed by the invention is an algal polysaccharide-based co-drug-loaded nano particle. The algal polysaccharide-based nanoparticles encapsulate anti-tumor hydrophilic drugs and hydrophobic drugs to realize targeted co-delivery of the drugs in solid tumor tissues, self-assembly is generated through electrostatic interaction of polysaccharide molecules to drive the encapsulated drugs to enter inner cores of the nanoparticles, and meanwhile biotin molecules are mainly distributed in shell layers of the nanoparticles. According to the algal polysaccharide-based co-drug-loading nano particle, the targeting property to solid tumor tissues is enhanced, the defect that a conventional drug-loading system can only encapsulate a single hydrophilic or hydrophobic drug is innovatively overcome, efficient co-delivery of hydrophilic and hydrophobic drugs with different attributes is realized, and proliferation and migration of tumors can be remarkably inhibited.

1. Name of the product in this design: Packaging Box (Zinc Biotin Vitamin). 2. Purpose of this design: As a packaging box. 3. The key design elements of this product are the combination of shape, pattern, and color. 4. The image or photograph that best illustrates the design's key points: a 3D model. 5. The design for which protection is sought includes color.

The present invention relates to an oral pharmaceutical composition comprising (i) a biologically active material conjugate in which a biological active material is conjugated with a biotin moiety, a

The invention relates to a protein detection method, in particular to an aptamer modified double-chain magnetic bead and application thereof in detection of beta-LG or hydrolytic peptide thereof. The invention discloses a biotin-labeled aptamer, and the sequence of the biotin-labeled aptamer is as shown in SEQ ID NO: 1 (5 '-TTTTTTGCAGTACCCACCCAGCCCAACATATGC-3'), and the sequence of the biotin-labeled aptamer is as shown in SEQ ID NO: 1. The aptamer is Apt TC obtained by trimming and optimizing BLG14, and the structure of the aptamer enables the aptamer to be capable of being combined with complete beta-LG and a hydrolysis peptide fragment thereof. According to the biotin-labeled aptamer and the rapid and low-cost milk detection method implemented by using the biotin-labeled aptamer, milk residues in a production line and food can be rapidly detected, and the situation that due to a lagged detection result, after a large amount of food is produced by an enterprise, the food is destroyed and lost due to disqualification is avoided.

The invention discloses a test strip biosensor for detecting uranyl ions, the test strip biosensor comprises a reaction system and a test strip, the reaction system comprises a DNAzyme substrate chain and a DNAzyme enzyme chain; the test strip comprises a substrate, a sample pad, a coupling pad, a nitrocellulose membrane and an absorption pad, the sample pad, the coupling pad, the nitrocellulose membrane and the absorption pad are sequentially overlapped on the substrate, a gold nanoparticle-DNA probe I compound is fixed on the coupling pad, a test line and a control line are arranged on the nitrocellulose membrane, an SA-biotin-DNA probe II is fixed on the test line, and an SA-biotin-DNA probe II is fixed on the control line. And an SA-biotin-DNA probe III is fixed on the control line. Compared with the prior art, the biosensor can realize high-sensitivity and high-specificity detection of uranyl ions by integrating a DNA enzyme induced cleavage reaction and a gold nanoparticle mediated colorimetric detection technology, the detection process is simple and rapid, and an important technical support is provided for field monitoring of UO2 < 2 + > in environment and biological samples.

Provided herein are compositions and methods for measuring the levels of flavins (e.g., in biological samples). In particular, provided herein are modified biotin-riboflavin conjugates and uses thereof (e.g., to measure flavin levels).

The invention discloses a method for detecting the conformational change of an APO-Cascade protein. Through mutation of Cas8 protein amino acid N376 and Cas5 protein K169 in a Casade protein, the obtained Cas8 and Cas5 labeled by non-natural amino acids can maintain functions, and at the same time, the mutant type of the Cas5 / 8 protein can be subjected to fixed-point fluorescence labeling; according to the method, the Cas6 is used as a template, the N end of the Cas6 is further modified with biotin, and a formed Casade compound can be fixed on a biotin-PEG functionalized single-molecule slide, so that the conformation state of the Casade protein can be truly reflected by using single-molecule fluorescence resonance energy transfer, an effective means is provided for in-vitro research on the structure-function relationship of a system in CRISPR / Casade, and the method has a wide application prospect. And the method is of great significance in designing the Cascade protein with optimized properties and enabling the Cascade protein to play new functions.

The invention discloses a bridging antibody, an enzyme-labeled polymer as well as a preparation method and application of the bridging antibody. The bridging antibody is prepared by the following steps: performing enzyme digestion, purification, reduction, sulfydryl protection and coupling on a polyclonal antibody and an anti-DMT-biotin monoclonal antibody through pepsin, and then purifying to obtain a heterodimer product polyclonal antibody-Fab '-Linker-Fab'-anti-DMT-biotin; hRP is subjected to maleimide and then coupled with a sulfhydrylated polymer to obtain a polymer enzyme, and then the polymer enzyme is coupled with a small molecule DMT-biotin to obtain the enzyme-labeled polymer which is specifically combined with the bridging secondary antibody. The obtained bridging antibody and enzyme-labeled polymer are used for preparing a kit for three-stage amplification of immunohistochemical signals, through cooperation of all the components, low-abundance antigens in tissues can be better detected, and the false positive rate is greatly reduced.