7728results about "Solid sorbent liquid separation" patented technology

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling compared with other methods.

A process for upgrading a plastic derived oil includes providing the plastic derived oil comprising hydrocarbons and greater than or equal to 100 ppmw halogen-containing compounds and contacting the plastic derived oil with an MMO catalyst in a first reactor at reaction conditions to remove halogens from the plastic derived oil to produce a first reactor effluent with less than 100 ppmw halogen-containing compounds. The process includes contacting the first reactor effluent with an adsorbent to produce a treated first reactor effluent, passing the treated first reactor effluent to a second reactor, and contacting the treated first reactor effluent with a cracking catalyst in the second reactor. Contacting with the cracking catalyst may cause hydrocarbons in the treated first reactor effluent to undergo catalytic cracking to produce a second reactor effluent comprising light olefins, naphtha range hydrocarbons, or combinations thereof.

The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.

The invention discloses a pipeline combined type single-autorotation intelligent counter-current chromatograph, and relates to the technical field of counter-current chromatographs, the pipeline combined type single-autorotation intelligent counter-current chromatograph comprises a rotating disc, a surrounding separation channel is formed in the rotating disc, a filling block is mounted in the surrounding separation channel, one end of the rotating disc is rotatably mounted through a liquid slip ring, and the other end of the rotating disc is rotatably mounted through a liquid slip ring. The other end of the rotating disc is connected with a main shaft, the main shaft is connected with a driving device, unwinding is conducted in a liquid slip ring mode, the rotating disc can complete unwinding action under single rotation, mixing of two phases in the separation channel can be enhanced through a filling block, and therefore arrangement of a planetary gear train can be abandoned; the main shaft is directly driven by the driving equipment to rotate, and the main shaft drives the rotating disc to rotate to realize two-phase separation and extraction, so that a higher rotating speed can be obtained, and the separation efficiency and effect are improved; and secondly, after the planetary gear train is abandoned, the equipment noise is greatly reduced, and good dynamic balance is achieved.

A modular porous swale. The modular porous swale may comprise a porous concrete block and filtration joint. The porous concrete block may snugly fit within a trench having a lower portion filled with an absorption media. The absorption media may include gravel, activated alumina, bone char, biochar, bone char and activated alumina mixture, and biochar and activated alumina mixture. The porous concrete block may have a top surface inwardly-sloped to a nadir. The filtration joint may be disposed within the porous concrete block and along the nadir. The liner may cover one or more sides of the porous concrete block. The perforated pipe may be located within the absorption media. The filtration joint may substantially align above a portion of the perforated pipe when the modular porous swale is installed within the trench.

The invention belongs to the field of blood product separation, and discloses a high-efficiency chromatography system and method for blood product separation, and the system comprises an intelligent chromatography column module, an automatic sample injection and accurate flow rate control module, a chromatography filler and column design module, a real-time detection and data analysis module, and an intelligent automatic control and management module. By designing an efficient micro-nano filler chromatographic column and matching with a pulsed electric field auxiliary technology and intelligent automatic control, efficient and high-purity separation and purification of target blood product components are realized. The intelligent regulation and control module used in the invention can dynamically regulate chromatography conditions in real time, and the problems of unstable flow velocity and column pressure in the traditional method are thoroughly solved. And meanwhile, a multi-point real-time detection and feedback technology is adopted, so that the separation process is more accurate, transparent and controllable.

The phosphazene covalent organic framework (COF) is a material with high efficiency to improve the removal of iodine from nuclear waste. The COF can be obtained by a solvothermal reaction of hexa(4-formyl-phenoxy)cyclotriphosphazene and 1,3,6,8-tetra(aminophenyl)pyrene. The resulting three-dimensional phosphazene COF has iodine uptakes as high as 9.4 g g−1 due to its 3D framework with higher specific surface areas and interconnected channels.

The invention discloses a halloysite / zirconium phosphate nano composite material, a preparation method thereof and application of the halloysite / zirconium phosphate nano composite material in heavy metal adsorption, and belongs to the technical field of materials. The halloysite / zirconium phosphate nano composite material is formed by growing nano zirconium phosphate particles on the inner wall of a halloysite nanotube, and the preparation process of the halloysite / zirconium phosphate nano composite material comprises the following steps: dispersing the halloysite nanotube in a solvent, adding a zirconium salt solution, carrying out ultrasonic treatment to obtain a halloysite-zirconium ion dispersion liquid, carrying out circulating vacuum treatment on the halloysite-zirconium ion dispersion liquid, and carrying out vacuum drying to obtain the halloysite / zirconium phosphate nano composite material. And carrying out solid-liquid separation, putting the obtained solid into phosphoric acid or a phosphate solution to carry out phosphating reaction, and washing and drying the obtained product to obtain the product. Zirconium phosphate nanoparticles in the composite material are small in size, uniform in distribution and good in loading stability, show high adsorption activity on heavy metal ions, can be widely applied to adsorption of the heavy metal ions in a solution system, are good in stability and can be repeatedly used for multiple times.

The invention relates to a post-treatment method of a vinyl polymer and the vinyl polymer obtained by the method. According to the post-treatment method disclosed by the invention, the post-treatment method comprising a washing step and an adsorption step is adopted, so that the ash content in the vinyl polymer can be effectively reduced in a simple manner.

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling compared with other methods.

The present invention provides a zirconium-based metal-organic framework material and a preparation method therefor and the use thereof, and an adsorption separation device and method. The zirconium-based metal-organic framework material has a chemical structural formula of [C18H6O16Zr3]n, and comprises zirconium and an organic ligand forming a coordination bond with zirconium, wherein the organic ligand is diphenylethyne-3,3′,5,5′-tetracarboxylic acid. The molecular structure of the zirconium-based metal-organic framework material of the present invention is a three-position network structure having a one-dimensional channel; and in the present invention, the size of the one-dimensional channel is accurately controlled by changing the aspect ratio of the organic ligand, such that the zirconium-based metal-organic framework material efficiently separates a hexane isomer by means of a kinetic effect.

Described herein are methods of recovering lithium from dilute lithium sources. The methods include extracting lithium from an extraction feed using direct lithium extraction in an extraction stage to yield a lithium intermediate, performing one or more concentration operations, each concentration operation concentrating an input stream to yield an output feed, wherein the input stream is obtained from the lithium intermediate and / or the extraction feed is obtained from the output feed. At least one of the concentration operations includes a membrane separation operation having a plurality of reactors in series each having a semi-permeable membrane, such as a counter-flow reverse osmosis operation. Methods may also include generating a low TDS stream as a permeate from any of the one or more concentration operations, wherein the low TDS stream is recycled or used as fresh water.

The present invention relates to the extraction of lithium from liquid resources such as natural and synthetic brines, leachate solutions from clays and minerals, and recycled products.

Provided herein are ion-selective separation membranes including a polymer matrix and a metal compound dispersed within the polymer matrix. The metal compound includes HaLibXcOa, where a is from 1 to 1.5, b is from 0 to 0.1, c is from 1 to 2, d is from 4 to 4.5, and X includes manganese or titanium.

The invention relates to a novel preparation method of tetra (dimethylamino) tin. The method comprises the following steps: in a nitrogen atmosphere, pumping an n-hexane solution of n-butyllithium and an n-hexane solution of dimethylamine into a pipeline through a propeller, mixing through a T-shaped joint, and reacting through a flow reactor 1 and a flow reactor 2 to obtain a lithium dimethylamide mixed solution; the method comprises the following steps: pumping a lithium dimethylamino mixed solution and a n-hexane solution of tin tetrachloride into a pipeline through a propeller, mixing through a T-shaped joint, and reacting through a flow reactor 3 and a flow reactor 4 to obtain a crude tetra (dimethylamino) tin product; and centrifuging and filtering the tetra (dimethylamino) tin crude product, adsorbing impurities by adopting a resin column, and carrying out reduced pressure distillation to remove the solvent, thereby obtaining the tetra (dimethylamino) tin. The adsorption resin column adopts modified macroporous adsorption resin, and is obtained by reacting, filtering and drying D190 macroporous styrene sulfydryl resin, dichloroethane, tetramethylguanidine, 5-allylpyrimidine-4, 6-diol and an ethylene boric anhydride pyridine complex.

The invention provides a therapeutic drug monitoring kit and method and system, a magnetic bead extraction method is combined with LC-MS / MS detection to be applied to human blood drug concentration monitoring, a mixed magnetic bead solution containing phospholipid removal magnetic beads and to-be-detected drug adsorption magnetic beads is utilized to perform adsorption and extraction pretreatment on a to-be-detected drug in a sample, automatic treatment equipment is matched, and the concentration of the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug is detected. The pretreatment efficiency is high, the phospholipid content in the supernate is low, and the matrix effect and the chromatographic column washing pressure are greatly reduced. According to the therapeutic drug monitoring method provided by the invention, a unified pretreatment process is used, simultaneous extraction of multiple drugs is realized, and through a magnetic bead automatic extraction method, the pretreatment step of a sample can be rapidly completed, a therapeutic drug monitoring result can be rapidly obtained, the overall detection time is shortened, and the detection efficiency is improved. Therefore, a reliable laboratory examination basis is provided for individualized treatment.

An exosome extraction device, comprising: a base (4); a filter column placement disc (1), wherein the filter column placement disc (1) comprises a placement disc body (11), a plurality of extraction column placement holes (111) are formed in the placement disc body (11), the extraction column placement holes (111) are used for placement of exosome extraction columns (100), and the placement disc body (11) can be rotatably mounted on the base (4) relative to the base (4); an exosome collection disc (2), wherein the exosome collection disc (2) comprises a collection disc body (21), a plurality of collection tube placement holes (211) are formed in the collection disc body (21), the collection tube placement holes (211) are used for placement of exosome collection tubes (200), and the collection disc body (21) is rotatably mounted on the base (4) relative to the base (4); and a collection disc translation mechanism for driving the collection disc body (21) to move to a position below the placement disc body (11) or move away from the position below the placement disc body (11), wherein in the state where the collection disc body (21) is located below the collection disc body (11), the placement disc body (11) and the collection disc body (21) rotate, so that the collection tube placement holes (211) can be aligned with the extraction column placement holes (111) so as to collect exosomes dripping from the exosome extraction columns (100).

A method for purifying vesicles formed from cells is provided. The method may include filtering a suspension comprising the vesicles to separate the vesicles from particles larger than the vesicles, wherein following the filtering, the separated vesicles are present in a volume smaller than the volume of the suspension, thereby producing filtrate comprising a concentrated vesicle suspension; concentrating the filtrate across a semi-permeable membrane against a super-absorbent polymer (SAP), wherein the SAP removes water from the filtrate, thereby producing a concentrated vesicle solution having a volume smaller than the volume of the filtrate; and subjecting the concentrated vesicle solution to size exclusion chromatography (SEC) and collecting a fraction comprising the vesicles, wherein the volume of the fraction is smaller than the volume of the concentrated vesicle solution.

The utility model discloses a solid-phase extraction filling device which comprises a shell, a material box is fixedly connected to the shell, a plurality of discharging holes are formed in the material box, a first partition plate and a second partition plate are fixedly connected in the shell, a feeding mechanism is arranged between the first partition plate and the second partition plate in a sliding mode, and a plurality of first via holes are formed in the first partition plate. The first via hole is communicated with the discharging hole, a plurality of second via holes are formed in the second partition plate, and the first via hole and the second via holes are completely staggered; the material receiving assembly comprises a base, a mounting frame is elastically arranged in the base, a plurality of hollow column pipes are placed on the mounting frame, a limiting seat is fixedly connected into the shell, the limiting seat is matched with the base and located below the second via hole, and a plurality of vibration motors are fixedly connected into the mounting frame. According to the utility model, a plurality of hollow column tubes can be quickly filled, so that a solid-phase extraction column is obtained, and an adsorbent material is more uniform and compact through the vibration motor.

Provided is a two-membrane eluent generator and a chromatography detection apparatus. The two-membrane eluent generator includes a stock solution channel; an eluent channel, a first ion-exchange membrane set being arranged between the eluent channel and the stock solution channel, the first ion-exchange membrane set including at least one ion-exchange membrane; a regenerant solution channel, a second ion-exchange membrane set being arranged between the eluent channel and the regenerant solution channel, the second ion-exchange membrane set including one bipolar membrane, wherein hydrogen ions or hydroxide ions generated at the bipolar membrane from electrolysis of a regenerant solution respectively enter different sides of the bipolar membrane, and impurity ions are prevented from entering the eluent channel through the bipolar membrane. The chromatography detection apparatus provided in present disclosure includes the two-membrane eluent generator. The present disclosure applies a bipolar membrane between the regeneration channel and the eluent channel, and is thus capable of supplying corresponding hydrogen ions or hydroxide ions for the eluent channel while preventing impurity ions in the regenerant solution from entering the eluent channel, thereby increasing the purity of the eluent.

The application discloses a chromatography material raw material composition, a chromatography material and a preparation method and application thereof, a whole column and a chromatography pile. The chromatography material raw material composition, with the total weight of raw material A and pore former B being 100%, comprises the following components: 20%-70% of raw material A; 30%-80% of pore former B; 0.1 ‰-1 ‰ of catalyst C; and the raw material A comprises a substance containing a polycyclic epoxy group. The chromatography material composition of the application can solve the pain points of the application of the current chromatography microsphere filler on biological macromolecules. The chromatography material has the advantages of uniform internal structure, good preparation reproducibility, high toughness, modifiable functional ligand and high hydrophilicity, and is more suitable for the separation and purification of biological molecules.

The invention discloses a pipeline-assembled single-rotation intelligent countercurrent chromatograph, which relates to the technical field of countercurrent chromatographs, and comprises a rotating disk, wherein a surrounding separation channel is provided in the rotating disk, wherein a filling block is installed in the surrounding separation channel, wherein one end of the rotating disk is rotatably installed through a liquid slip ring, and the other end of the rotating disk is connected to a main shaft, wherein the main shaft is connected to a driving device, and unwinding is performed by means of a liquid slip ring, so that the rotating disk can complete the unwinding action under single rotation and can rely on the filling block to strengthen the mixing of two phases in the separation channel, thereby abandoning the setting of a planetary gear system, and the main shaft directly drives the main shaft to rotate through the driving device, and the main shaft drives the rotating disk to rotate to realize two-phase separation and extraction, thereby being able to obtain a higher rotation speed and improving the efficiency and effect of separation; secondly, after abandoning the planetary gear system, the equipment noise is greatly reduced, and the device has good dynamic balance.

The utility model belongs to the technical field of sugar alcohol preparation, and relates to a system for co-producing L-arabinose and caramel pigment by using xylose chromatography raffinate, which comprises a raw material tank, a nanofiltration membrane separator, a chromatography separation device, an evaporation concentration tank, a crystallization tank, a centrifugal separator and a dryer which are sequentially communicated by pipelines, and the browning reaction assembly is used for mixing the raffinate and the centrifugal mother liquor and then carrying out browning reaction treatment to prepare caramel pigment. The chromatographic separation device is used for separating an extracting solution and raffinate from the flowing nanofiltration permeate, the evaporation concentration tank and the crystallizing tank are used for concentrating and crystallizing the flowing extracting solution into massecuite, the centrifugal separator is used for separating the flowing massecuite into moist sugar and centrifugal mother liquor, and the dryer is used for drying the flowing moist sugar into L-arabinose crystals. According to the utility model, raffinate and centrifugal mother liquor are mixed to prepare a caramel pigment liquid product, so that a caramel pigment additional product is obtained while the utilization rate of saccharide resources is improved.

The invention discloses a method for green and efficient removal of thiophene sulfides in fuel oil by using Q [8]. According to the method provided by the invention, a Q [8] solid powder material is adopted to carry out removal treatment on simulated fuel oil containing the thiophene sulfides in a subject-object interaction mode, and the Q [8] solid powder material can be subjected to a regeneration process for reuse through ethanol desorption. The Q [8] solid powder material used in the method is low in cost, the urea is a main raw material for synthesis of the Q [8] solid powder material, and the Q [8] solid powder material is high in selectivity, can be recycled and is more environmentally friendly. The method is simple to operate, convenient to use and low in cost, the combustion simulation oil can be efficiently desulfurized, and environmental pollution is effectively reduced.

The invention belongs to the technical field of lithium carbonate, and relates to battery-grade lithium carbonate and a preparation method thereof.The preparation method comprises the following steps that S1, lithium precipitation mother liquor is decarburized, and after decarburization, the lithium precipitation mother liquor is sequentially evaporated, concentrated and filtered to obtain concentrated liquor; s2, preparing a chelating resin for removing calcium and magnesium in a high sodium sulfate salt environment; s3, enabling the concentrated solution to pass through chelating resin to obtain a calcium and magnesium removed concentrated solution; s4, performing lithium precipitation on the sodium carbonate solution and the concentrated solution to obtain lithium carbonate slurry, and pumping the lithium carbonate slurry into an intelligent cutting sharpening system for circular shearing; and S5, lithium carbonate is filtered out after cyclic shearing, then two-stage washing is conducted, and secondary cyclic shearing is conducted through an intelligent cutting sharpening system in the second-stage washing process. According to the method disclosed by the invention, a traditional carbonization purification process is abandoned, a two-time cyclic shearing method is utilized, peritectic is opened for a traditional lithium precipitation system, preservation residues are solved, the content of sodium and sulfate radicals is effectively reduced, and a battery-grade lithium carbonate product is finally obtained.

The invention provides a method for preparing high-purity phosphatidylserine by a batch enzyme catalysis method based on dynamic choline removal, and belongs to the technical field of enzymatic reaction. According to the method disclosed by the invention, in a reaction system for preparing phosphatidylserine, a byproduct choline is selectively adsorbed by adopting resin. Choline in a reaction system is removed in real time through the integrated adsorption separation module, the enzyme inhibition problem is solved, and meanwhile, the PC conversion rate is larger than or equal to 98% and the PS purity is larger than or equal to 96% in combination with the immobilized enzyme circulation technology; the enzyme can be reused for more than or equal to 20 times (activity retention is more than or equal to 90%); and the choline residual concentration is less than or equal to 0.5 mM (an inhibition threshold value or less).

Apparatuses and methods for extracting desired chemical species including, without limitation, lithium, specific lithium species, and / or other chemical compounds from input flows in a modular unit. The input flows may be raw materials in which lithium metal and / or lithium species are dissolved and / or extracted. The apparatuses and methods may include daisy chain flow through separate tanks, a column array, and / or combinations thereof.

Disclosed are chromatography systems employing a releasable coupling 100 for holding a fluid tubing to a spigot, the coupling comprising: a cylindrical inner component 110 for accepting a fluid tubing 30, said inner component including a resiliently deflectable portion 118 arranged to urge an outer surface of the tubing toward a spigot; and a cylindrical collar 130 having and internal through-aperture 132 for slideably accepting the inner component, the aperture and resilient portion having complementary surface formations which in a first position of the collar mounted to the inner component provide for said resilient deflection in use, and which in a second different position do not cause said deflection, the coupling being characterized in that the collar comprises a collar flange 134 extending outwardly away from the aperture of a size allowing manual manipulation of the collar between the first and second positions. Chromatography systems employing said couplings is disclosed also.

The invention discloses a white spirit flavor substance solid-phase micro-extraction device and method, and relates to the technical field of micro-extraction devices.A heat insulation partition plate is fixedly arranged on the inner wall of a tank body, an extraction subarea is arranged on the left side of the heat insulation partition plate in the tank body, and a desorption subarea is arranged on the right side of the heat insulation partition plate in the tank body; the heating unit is fixedly arranged on the lower inner wall of the tank body, a guide groove is formed in the cover plate, the mounting sleeve is movably arranged in the guide groove in a penetrating mode, a mounting groove is formed in the heat insulation partition plate, the two sealing plates are arranged on the upper side and the lower side of the cover plate respectively, and the mounting sleeve is fixedly arranged on the sealing plates on the upper side and the lower side in a penetrating mode; according to various extraction and enrichment modes in the micro-extraction technology, integrated structural equipment is designed, two mutually independent partitions are arranged in the equipment, so that extraction and desorption operation can be realized, and furthermore, a lifting adjusting structure and a heating structure are arranged in the equipment, so that micro-extraction operation in different modes can be selected.

The invention belongs to the technical field of chromatographic packing, and particularly relates to a double-layer hole type SiO2 (at) SiO2 core-shell microsphere based on a double-phase method and a preparation method and application of the double-layer hole type SiO2 (at) SiO2 core-shell microsphere. According to the core-shell microsphere, micron-sized non-porous SiO2 serves as a core, a mesoporous SiO2 framework with pore channels perpendicular to the surface of the non-porous SiO2 core serves as a porous shell layer, the porous shell layer sequentially comprises a first ordered mesoporous shell layer and a second ordered mesoporous shell layer which are different in pore diameter from inside to outside, and the pore channels of the two shell layers are completely consistent in direction and are communicated with each other. According to the invention, a porous shell structure with dual or multiple pore size distribution is constructed on the surface of a SiO2 core, and by integrating more than two pore sizes with obvious size difference, exclusive mass transfer paths are provided for solute molecules with different sizes, exclusive mass transfer channels can be provided for small-molecular compounds and macromolecular substances at the same time, and the pore size difference is greatly reduced. Therefore, the selectivity and column efficiency of chromatographic separation are remarkably improved.