100results about How to "Retain activity" patented technology

The invention relates to the technical field of drying process control, in particular to a medicinal material drying process control method and system for traditional Chinese medicinal material processing, and the method comprises the following steps: calling a temperature set value, a humidity discharge rate and heating power based on the operation condition of a drying chamber, and analyzing the difference value between the medicinal material moisture content and the target moisture content; combining the medicinal material density with the water content change rate, matching the coupling relationship between the humidity discharge rate and the heating power, and establishing an initial drying process control value; according to the method, through collaborative analysis of the temperature set value, the humidity discharge rate and the heating power, accurate matching of initial drying conditions and material characteristics is achieved, excessive drying is avoided, linkage of the air speed and the humidity discharge rate is optimized, humidity distribution uniformity and environmental stability are improved, the evaporation rate is dynamically matched, and the drying efficiency is improved. Drying uniformity and quality stability are improved, termination control parameters are optimized in real time, drying time is shortened, energy consumption is reduced, and active ingredients and physical characteristics of medicinal materials are reserved to the maximum extent.

The invention relates to the technical field of biochemical engineering and food processing, in particular to a fish collagen extraction process which comprises the following steps: S100, raw material pretreatment; s200, carrying out crushing and enzymolysis pretreatment; s300, carrying out an enzymatic hydrolysis reaction; s400, carrying out solid-liquid separation; s500, carrying out concentration treatment; and S600, drying treatment is carried out. The advanced spectrum analyzer is used for accurately determining the freshness of the fish body, the automatic cutting and ultrasonic cleaning technologies are combined, the quality of raw materials is effectively guaranteed, the enzymolysis reaction conditions are strictly controlled through the high-precision metering pump and the heat exchange system, the enzymolysis efficiency and the product quality are improved, and the production cost is reduced. By adopting a multi-layer composite membrane structure and a specific concentration technology, the efficiency and purity of solid-liquid separation and concentration treatment are remarkably improved, the production process is optimized, the energy consumption and the cost are reduced, pollutants in the production process are effectively removed through application of a plasma cleaning and air purification system, and the product quality is improved. And reliable guarantee is provided for production of high-quality fish collagen products.

The invention provides double-crosslinking acellular matrix hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: (1) carrying out decellularization treatment on a porcine dermal layer to obtain a decellularized porcine dermal matrix, (2) putting freeze-dried powder of the decellularized porcine dermal matrix into an acidic pepsin solution, stirring and digesting, adjusting the concentration, adding 10xPBS, adjusting the pH value to obtain a pregel solution, and carrying out freeze-drying on the pregel solution to obtain the porcine dermal matrix freeze-dried powder. (3) putting gel formed by the pre-gel solution into a tannic acid solution for cross-linking to obtain mono-cross-linked acellular matrix hydrogel; (4) smashing the mono-cross-linked acellular matrix hydrogel, and mixing the smashed mono-cross-linked acellular matrix hydrogel with a glucose oxidase solution and a ferrous ion solution with the concentration of 0.1-10mg / ml to obtain a bi-cross-linked acellular matrix hydrogel precursor; 5, the double-crosslinking acellular matrix hydrogel precursor is placed under the condition of glucose with the concentration being 10-200 mg / dl to form the double-crosslinking acellular matrix hydrogel. The hydrogel prepared through the method can achieve the balance of safety, supporting performance and degradability, and is wide in application range.

The invention provides a skin care composition with an anti-aging effect and a preparation method and application thereof, and belongs to the technical field of beauty and skincare, the composition is characterized in that retinol, a polypeptide compound, ceramide, astaxanthin and resveratrol have a synergistic effect on component combination, so that all-around anti-aging is realized, and the skin care effect is good. According to the present invention, the raw materials are respectively adopted to provide effects of collagen generation promotion, muscle contraction wrinkle solving, barrier function enhancing, free radical scavenging, melanin formation inhibition and the like, the preparation method comprises the following steps: precise temperature control, suitable stirring mode, high-precision stirrer and other advanced technologies ensure uniform mixing of the components, inert gas protection and PID temperature controller retention of the activity and the effects of the components, in the aspect of application, the composition is reasonably added into face cream, emulsion, essence and facial mask, diversified requirements are met, deep nourishing, refreshing care, efficient permeation and instant effects are respectively provided, in a word, an efficient, safe and comprehensive anti-aging scheme is provided for consumers, aging signs are improved, and the composition is wide in prospect and high in value.

The invention belongs to the technical field of plant drinks, and particularly relates to kidney-tonifying and bowel-relaxing cistanche tea and a preparation method thereof. The herba cistanche tea is prepared from the following raw materials in parts by weight: 30 to 40 parts of herba cistanche, 10 to 15 parts of fructus lycii extract, 10 to 12 parts of semen cassiae extract, 10 to 15 parts of fructus cannabis extract, 8 to 12 parts of radix polygoni multiflori extract, 8 to 10 parts of rhizoma dioscoreae extract, 3 to 5 parts of honey powder, 5 to 10 parts of maltodextrin, 2 to 3 parts of beta-cyclodextrin, 2 to 3 parts of antioxidant, 0.1 to 0.15 part of mixed enzyme and 2 to 3 parts of zymophyte. According to the preparation method disclosed by the invention, the flowers and the rhizomes of the cistanche are subjected to pretreatment and enzymolysis treatment in different modes, and then fermentation is performed under the same microbial fermentation condition, so that respective advantages of the two parts of raw materials are fully played, beneficial components are released and retained to the maximum extent, and the functionality and the quality of the product are remarkably improved through synergistic fermentation.

The invention relates to an itching relieving and soothing skin care lotion and a preparation method thereof. The skin care lotion is prepared from the following raw materials in parts by weight: 10-20 parts of grease, 1-3 parts of a nonionic emulsifier, 0.1-1 part of a skin feeling regulator, 0.5-2 parts of a first skin conditioner, 1-5 parts of a second skin conditioner, 0.1-1 part of an antioxidant, 1-2 parts of a humectant, 0.1-1 part of a pH regulator and 70-85 parts of water, the first skin conditioner contains a citrus peel extract and carboxymethyl chitosan, and the second skin conditioner contains hydroxyphenyl propionamide benzoic acid, stearyl glycyrrhetinate, menthol lactate, alpha-bisabolol and guaiazulene. The skin care lotion does not contain diphenhydramine hydrochloride and other substances with safety risks, is safe and mild, has remarkable itching relieving and soothing effects, and is safe and non-irritant to infants and children; during preparation, heating is little, and the activity of active substances can be reserved to the maximum extent so as to effectively ensure the itching relieving and relieving effects of the product.

The invention relates to an extraction and purification method of green tea cell wall polysaccharide with hypoglycemic activity and the obtained green tea cell wall polysaccharide. The green tea is extracted by using water, CDTA, saturated sodium carbonate, 1M NaOH solution and 4M NaOH solution through a sequential extraction method to obtain five different tea polysaccharides; the green tea cell wall polysaccharide with the best hypoglycemic activity is screened out through activity inhibition of glycometabolism enzyme, and is further purified by gel column chromatography. Compared with the prior art, the method has the advantages that the reaction conditions in the whole operation process are mild, the activity of the green tea cell wall polysaccharide can be reserved to the greatest extent, the loss of polysaccharide components is reduced to the greatest extent, the extraction rate is high, the energy consumption is low, the solvent dosage is small, the operation is simple, and the economic cost is low; a foundation is laid for researching the physicochemical property and biological activity of the green tea cell wall polysaccharide, and the method has important significance on utilization of the green tea cell wall polysaccharide in the fields of food, medical care, cosmetics and the like.

The invention provides a plant composition capable of controlling oil, resisting inflammation and relieving. The composition comprises the following raw materials in parts by mass: 3-5 parts of a centella asiatica extract, 1-2 parts of a liquorice extract, 2-4 parts of a clerodendranthus spicatus extract, 1-4 parts of a willow herb extract, 2-5 parts of a trifolium pretense flower extract, 4-6 parts of a cymbopogon citratus leaf extract, 7-9 parts of a multiflora rose root extract and 0.1-0.5 part of a vitamin compound. The multiflora rose roots are extracted by adopting reflux extraction, enzymolysis and fermentation technologies, active substances in the multiflora rose roots are fully extracted, the obtained multiflora rose root extract has a synergistic effect with other raw materials, and excellent oil control, anti-inflammation and soothing effects are provided for skin.

The invention belongs to the technical field of cell engineering and biochemical detection, and particularly relates to an erythrocyte membrane with a complete structure and a preparation method and application thereof. The red blood cells are split by limiting the components of the release liquid, the conditions are mild, protein components such as hemoglobin in the red blood cells can be released, the cell attributes are removed, the integrity of the internal skeleton structure and the activity of the membrane surface antigen are reserved, the complete structure of the red cell membrane is kept, meanwhile, the key blood group antigen on the surface is reserved, and the effect is good. The problem of antigenicity reduction caused by erythrocyte membrane fragmentation is avoided. After the erythrocytes are lysed, the nanoparticles are coupled to the surface of the erythrocyte membrane, so that the agglutination reaction of the erythrocyte membrane under the induction of the antibody can be visualized. The engineering red blood cell biological membrane is preserved by adopting a fixed program, so that the damage of ice crystals to the engineering red blood cell biological membrane can be effectively avoided, the antigenicity of the membrane is not influenced, the membrane can be preserved for a long time at normal temperature, and the shelf life can be prolonged to two years or more.

The invention discloses a methyl modified hydrogel electrolyte, a preparation method thereof and application of the methyl modified hydrogel electrolyte in a zinc battery, and belongs to the field of secondary batteries. The hydrogel electrolyte is composed of acrylamide, methyl cellulose and zinc sulfate. The preparation method comprises the following steps: adding methyl cellulose and acrylamide into a zinc sulfate solution, uniformly mixing, then adding a potassium persulfate initiator and a cross-linking agent N, N '-methylene bisacrylamide, finally putting into a double-layer glass plate, sealing, and carrying out thermal polymerization. The methyl cellulose has zinc affinity and can convert an acrylamide proton polymer into an aprotic polymer to weaken intermolecular hydrogen bonds, so that free hydrogen bond receptors are released to participate in cationic solvation. The solubilization and recrystallization strategy can effectively adjust the gel electrolyte environment, cut off hydrogen bonds formed by binding water donors and participate in electrolyte salt dissolution, so that the electrolyte concentration is increased, and salting-out of the electrolyte is inhibited. When the gel electrolyte is applied to the zinc battery, zinc ion migration can be accelerated, and excellent cycle performance under a low-temperature condition is realized.

The invention relates to a preparation process for extracting high-content chlorogenic acid from green coffee broken rice. According to the process, a non-organic solvent method is adopted, the low-caffeine green coffee bean extract is prepared through the steps of raw material pretreatment, low-temperature ultrasonic enzymolysis extraction, column chromatography, concentration, spray drying and the like, and active ingredients of various caffeoylquinic acid substances such as chlorogenic acid are enriched. In the preparation process, a protective agent is used for avoiding attenuation of chlorogenic acid in heating and air, a resin gradient separation method is used for enriching chlorogenic acid to remove caffeine, and a UPLC technology is used for realizing simultaneous quantitative detection of various caffeoylquinic acid components. The preparation process has the advantages of no organic solvent residue, high effective component content, high separation efficiency, safety, environmental protection, and suitableness for large-scale production. The final product not only contains high chlorogenic acid and low caffeine, but also is rich in caffeoylquinic acid substances and other active ingredients, has better flavor than market products, and is lower in application difficulty in the food and beverage industry.

The invention discloses a semitransparent skin-nourishing face cream based on a medium-temperature hot pot process and a preparation method and application thereof, belongs to the technical field of cosmetics, and aims at solving the problems that in the prior art, in the face cream preparation process, a high-temperature processing process can destroy the activity of natural extracts; and the technical problem of how to enable the face cream to be semitransparent through formula system combination is solved. The preparation method of the semitransparent skin-care face cream based on the medium-temperature hot pot process comprises the following steps: putting a phase A into an emulsifying pot, and stirring and heating to 80-85 DEG C; adding the phase B into the emulsifying pot, stirring and heating to 80-85 DEG C, and then cooling to 60-65 DEG C; sequentially adding the phase A, the phase B, the phase C and the phase D into an emulsifying pot to enable the materials to be uniform, and cooling the materials to 53-57 DEG C to obtain cream; and filling the cream to obtain the prepared semitransparent skin-nourishing face cream based on the medium-temperature thermal tank process. The semitransparent face cream is prepared by designing a component compounding system.

The present invention belongs to the field of daily chemical new materials, and discloses a method for preparing a fermented facial mask liquid, wherein fermented bacteria are inoculated into a fermentation culture medium for fermentation treatment to obtain a fermented facial mask liquid; the fermentation culture medium contains a carbon source, a tremella extract, a yeast extract, rice, and wheat germ; the fermented bacteria are a 10-bacteria type bifidobacterium probiotic powder household lactic acid bacteria starter provided by Angel Yeast Co., Ltd. The method uses tremella extract, yeast extract, wheat germ, and rice for mixed fermentation, and the obtained fermentation product has a viscosity of more than 290 mPa·s. After dilution, the adjustable viscosity range is 100 to 300 mPa·s, and it still has the skin care functions of firming, soothing, and anti-wrinkle under low concentration conditions, and has good safety, mildness, and non-irritation. At the same time, the present invention also provides a fermented facial mask liquid and its application.

This utility model discloses a pulverizing and grinding device for the production of ester raw materials, including a main support unit, a ball mill at the top of the main support unit, a secondary pulverizing device on one side of the main support unit, a material collection unit at the top feed end of the secondary pulverizing device, and the discharge end of the ball mill above the material collection unit, allowing the discharge from the ball mill to enter the material collection unit. Air nozzles and air outlets are provided on both side walls of the secondary pulverizing device, and sealed conveying units are provided at both the top feed end and the bottom discharge end of the secondary pulverizing device. This utility model addresses the problems of localized temperature rise in existing grinding equipment leading to thermal decomposition of ester raw materials and insufficient grinding precision. It employs multi-stage pulverization, coarsely pulverizing followed by ultrafine grinding, reducing the energy consumption of a single device. Furthermore, a low-temperature medium is introduced during the fine grinding process for cryogenic pulverization or low-temperature grinding, inhibiting the thermal degradation of esters, thus meeting the high standards required in the food, pharmaceutical, and other fields.

The invention provides a method for inhibiting post-acidification of fermented food. Comprising the following steps: fermenting food raw materials to obtain a fermented product; and carrying out induction treatment on the fermented product, so that the probiotics in the fermented product enter a viable and non-culturable state. The method can effectively inhibit post-acidification of the fermented food, prolong the shelf life of the fermented food, reserve the activity of probiotics in the fermented food, maintain or improve the nutritional value of the fermented food and improve the quality of the fermented food, and is wide in application prospect.

The utility model relates to the technical field of injection devices, in particular to an SOD (superoxide dismutase) enzyme injection device which comprises a fixed bottom plate, a vertical plate fixedly arranged on one side above the fixed bottom plate, a liquid storage tank fixedly arranged above the vertical plate, a liquid injection port formed in the top of the liquid storage tank, a liquid outlet formed in the bottom of the liquid storage tank, and a soft tube connected with one end of the liquid storage tank in a sealed manner. A first one-way valve is arranged on the liquid outlet, an injector is movably connected to the other end of the soft tube, a supporting frame is installed on the other side of the upper portion of the fixed bottom plate, and an arc-shaped groove is formed in the top of the supporting frame and matched with the outer wall of the injector; a liquid storage chamber is arranged inside the liquid storage tank, the right end of the liquid storage chamber is fixedly connected with the right end of the inner wall of the liquid storage tank, and a cooling cavity is formed between the liquid storage chamber and the liquid storage tank. The SOD enzyme injection device can be used for storing SOD enzyme injection at low temperature and in a dark place, so that the SOD enzyme is prevented from thermal change and decomposition when encountering light, the activity of the SOD enzyme is reserved to a great extent, and the SOD enzyme injection device has the advantages of being simple in structure and convenient to use. And the injector is convenient to move, and the needle head is convenient to disassemble and replace.

The invention discloses a temperature-controllable pressurizing heat treatment device for producing and processing sea cucumber peptides, and relates to the technical field of sea cucumber peptide production equipment. The device comprises a heating bin, a heater is fixedly installed at the inner bottom of the heating bin, a temperature sensor is fixedly installed on the inner wall of the heating bin, two sets of linear sliding rail modules are rotatably installed at the top of the heating bin, a material frame is installed between the two sets of linear sliding rail modules, and multiple sets of material openings are formed in the bottom of the material frame; and the bottom of the material opening is fixedly connected with an elastic material bag. The sea cucumbers are put into the elastic material bag, then the elastic material bag is vacuumized through the negative pressure plate and the negative pressure head, then the vacuum elastic material bag is put into the heating bin, water bath heating is conducted through the vacuum bag, due to the existence of the vacuum environment, water evaporation is little, the taste and quality of products can be kept easily, and oxidation and water loss can be effectively prevented; therefore, active ingredients and nutrient substances in the sea cucumber peptide are better reserved.

The invention belongs to the technical field of plant beverage processing, and particularly relates to a gingko, dendrobium officinale and ginseng plant beverage crushing, mixing and preparing device which comprises a mixing and preparing machine, a universal pulverizer, an ultrafine pulverizer, a freezing pulverizer and a high-pressure homogenizer, and the mixing and preparing machine comprises a material cavity, a feeding opening, a discharging opening and a stirring mechanism; the preparation method of the mixing preparation machine comprises the following preparation steps: S1, raw material pretreatment: selecting ginkgo leaves, fresh dendrobium officinale strips and ginseng rhizomes which are free of mildew and diseases and insect pests, cleaning the ginkgo leaves, drying the ginkgo leaves with hot air, removing fibrous roots from the fresh dendrobium officinale strips, cutting the fresh dendrobium officinale strips into long sections, peeling the ginseng rhizomes, and cutting the peeled ginseng rhizomes into long slices; s2, graded crushing is conducted, specifically, the pretreated ginkgo leaves are put into a universal pulverizer to be pulverized and then transferred into an ultrafine pulverizer to be further pulverized into fine particles, and the device solves the problems that when the ginkgo, dendrobium officinale and ginseng plant beverage is prepared through crushing and mixing at present, the mixing effect is poor, and the stability and taste of the beverage are directly affected.

This utility model belongs to the field of traditional Chinese medicine extraction and separation technology. It discloses an integrated system for the extraction and separation of heat-sensitive components from traditional Chinese medicine, including a stirred extraction tank, a cooler, a cyclone centrifuge, a stirring device, a power delivery pump, an ultrasonic generator, and an air-lift device. The stirred extraction tank includes a tank body with a discharge port at the bottom and a jacketed cylinder on the side wall. An annular cavity is left between the jacketed cylinder and the tank body, with an inlet and an outlet on the cavity. The tank body is vertically arranged with multiple support legs. A first and second inlet are also provided at the top of the tank body. A stirring device is installed at the top of the tank body. Multiple ultrasonic generators are annularly installed along the side wall of the tank body. The cooler is located on one side of the stirred extraction tank. This utility model integrates stirring and ultrasonic extraction, jacketed hot and cold circulation, and a rapid liquid-residue separation system, significantly improving the extraction efficiency of effective components from traditional Chinese medicine, shortening the extraction time, and effectively protecting the activity of heat-sensitive components.

The invention relates to the technical field of active component extraction of rhizoma polygonati, in particular to a method for extracting and separating multiple active components in rhizoma polygonati, which comprises the following steps: step 1, raw material preparation: removing silt from rhizoma polygonati rhizome, cleaning, drying the cleaned rhizoma polygonati to constant weight, and grinding the dried rhizoma polygonati in a grinding centrifugal device; step 2, component extraction: performing ultrasonic-assisted pretreatment on the rhizoma polygonati; extracting flavonoid compounds in rhizoma polygonati by adopting an alcohol extraction method; extracting polysaccharide components in rhizoma polygonati by adopting an alcohol precipitation method; step 3, separation and purification: purifying the polysaccharide by using anion exchange column chromatography; and the flavonoid compounds are purified by using a macroporous resin adsorption method. According to the method disclosed by the invention, ultrasonic waves are utilized for pretreatment, so that the penetrating power of cell membranes is increased, and therefore, active substances in cells can easily permeate, and the purity and the quality of various active components in the finally extracted rhizoma polygonati are improved.

The invention provides a lactic acid bacteria preparation capable of being preserved for a long time at normal temperature and a preparation method thereof, and relates to the technical field of microorganisms, the lactic acid bacteria preparation successfully realizes long-time stable preservation at normal temperature through an optimized strain combination, an elaborately-prepared protective agent and an advanced preparation process, and the lactic acid bacteria preparation can be stored for a long time at normal temperature. Compared with a lactic acid bacteria preparation in the prior art that the activity is greatly reduced in a short time at normal temperature and the storage life is limited, the preparation disclosed by the invention can be stably stored for at least 9-15 months, so that the shelf life of the product is greatly prolonged, the high survival rate and stability of lactic acid bacteria are guaranteed, and the product quality is improved by accurately controlling the activation and culture conditions of the strain. According to the present invention, the temperature, the time and the bacterial liquid concentration are determined, the good growth state and the good activity of the lactic acid bacteria at the initial stage are ensured, the optimized bacterial strain combination enhances the product performance, and the specific combination of the lactobacillus plantarum, the lactobacillus acidophilus and the bifidobacterium is adopted to achieve the synergistic effect of the different bacterial strains;

The application discloses an extraction device for natural product separation, which comprises an extraction tank, a membrane separation assembly, a stirring motor, a temperature control assembly and a controller. The temperature control assembly adopts an up-down partitioned cold-hot temperature control design, and a heat insulation partition fence is arranged between the cooling pipe and the heating pipe. In combination with an anchor type gentle stirring, denaturation of active ingredients is avoided. The membrane separation assembly realizes three-stage gradient separation through a primary ultrafiltration membrane, a medium-level nanofiltration membrane and a high-level affinity membrane. The high-level affinity membrane is loaded with replaceable biological ligands to accurately enrich target components. A structure for real-time detection of concentration and activity of an extraction agent is matched, and the whole process is automatically regulated by electric control valves and the controller. The application can efficiently separate natural active substances of targeted membrane protein targets and drug resistance related targets, completely retains the activity of components, has high separation purity, high automation degree, and recyclable extraction agent, is suitable for laboratory research and development and small-scale industrial production, and meets the extraction requirements in the fields of biological experiments, agriculture and biological medicines.

The present invention relates to a modified sulfamidase, a composition comprising a modified sulfamidase, as well as methods for preparing a modified sulfamidase and the therapeutic use of such a sulfamidase. In particular, the present invention relates to a modified sulfamidase comprising virtually no epitopes for glycan recognition receptors, thus enabling the transport of said sulfamidase across the blood-brain barrier of a mammal, said sulfamidase having catalytic activity in the brain of said mammal.

The invention discloses a preparation method of inositol nicotinate tablets, and belongs to the technical field of pharmaceutical preparations. By optimizing the existing process and coating components, stable dissolution of drugs is realized, and the method comprises the following steps: dispersing inositol nicotinate in mixed oil to prepare an oil phase, mixing and emulsifying the oil phase and a water phase, performing spray drying to obtain drug milk powder, coating the drug milk powder with modified chitosan, and directly tabletting the coated drug milk powder and auxiliary materials. The preparation method comprises the following steps: uniformly dispersing inositol nicotinate in mixed oil to promote the inositol nicotinate to maintain the uniformity of the drug content in the subsequent process, and adsorbing solid particles on the surfaces of drug-containing oil drops through an emulsification technology to protect the drug components; coating components are reasonably designed, so that fixed-point release of the medicine is realized, the medicine is endowed with anti-pressure ability, and the medicine can be directly tableted; and the coated medicine particles are mixed with auxiliary materials and then are directly tableted, so that the process is simplified, and the coating structure of the medicine particles is protected at the same time. The preparation method can prepare the inositol nicotinate tablet with stable drug dissolution, and the inositol nicotinate tablet is good in taste and small in stimulation to gastric mucosa.

This invention discloses a stepwise continuous flow method for preparing Miraberon using a microchannel reactor. Specifically, the method involves synthesizing Miraberon from p-nitrophenylethylamine hydrochloride and D-mandelic acid, followed by amide condensation, borane reduction, hydrogenation reduction, and a second amide condensation reaction to obtain the target product. Each step utilizes a continuous flow process for intermediate synthesis. This invention offers high reaction safety, short reaction time, and simple post-processing. It fundamentally eliminates the safety hazards caused by hydrogen leakage, reduces catalyst friction loss due to stirring in batch reactions, and avoids catalyst contact with air during post-processing, thus preserving catalyst activity, improving catalyst utilization efficiency, and significantly reducing economic costs.

The invention relates to the field of beverage preparation, and discloses a functional compound beverage rich in vitamins and trace elements and a preparation method thereof, and the functional compound beverage comprises the following components in parts by weight: 3-7 parts of vitamins, 8-12 parts of minerals, 2-4 parts of a trace composition, 1-3 parts of an additive and 85-95 parts of water; the functional compound beverage provided by the invention is rich in various vitamins and trace elements and good in functionality, and the prepared functional compound beverage not only is long in shelf life and resistant to illumination and high temperature, but also can retain the activity and efficacy of the vitamins and the trace elements to the maximum extent.

This invention discloses a method for preparing Polygonatum sibiricum enzyme liquid, belonging to the field of bio-fermentation technology. The method includes: gradient permeation activation treatment of Polygonatum sibiricum, including negative pressure pulsating permeation and reduced pressure concentration under inert gas protection; preparation and co-inoculation of a composite strain of Lactobacillus plantarum and Saccharomyces cerevisiae propagated in a stepwise sequential manner; multi-stage temperature and pressure-coupled fermentation, including pressurized microaerobic primary fermentation and high-temperature static incubation for enhanced conversion; targeted induction of enzymatic hydrolysis and low-temperature maturation; and in-situ aseptic filling. This invention does not add any other ingredients throughout the entire process. Through a series of original, interrelated process steps and precise parameter control, it solves the technical problem of efficiently converting a single Polygonatum sibiricum raw material into a high-quality enzyme liquid, ultimately producing a pure Polygonatum sibiricum enzyme liquid with high active substance content, mellow taste, and excellent stability.

The application discloses a kind of hydrogel microneedle patches based on bletilla striata polysaccharide composite engineered exosome and its preparation method and application, belong to the technical field of biomedical materials, with bletilla striata polysaccharide and methacrylated hyaluronic acid as matrix, and the hydrogel microneedle with excellent mechanical properties is prepared.The natural polyphenol caffeic acid is used to modify the bone marrow mesenchymal stem cell exosome, and the engineered exosome is loaded on the microneedle tip by centrifugation.The strong antioxidant capacity of caffeic acid is used to protect the structure of exosome, and a synergistic system is formed, combined with the antibacterial and anti-inflammatory properties of the bletilla striata polysaccharide matrix itself, the diabetic wound microenvironment can be improved from multiple dimensions such as bacteriostasis, antioxidant, anti-inflammatory and pro-angiogenesis.The preparation process of the application is simple and controllable, the product has high biological safety, and the active ingredients can be delivered precisely and minimally invasively, providing an efficient and safe solution for diabetic chronic wound repair.

The application discloses a preparation method of enteric sustained-release defensin mesoporous silica nanoparticles. The enteric sustained-release defensin mesoporous silica nanoparticles prepared by the method have good defensin loading capacity, good responsiveness to trypsin and good enteric targeting and sustained-release defensin performance.

The invention discloses an extraction process of citrus essential oil and the citrus essential oil prepared through the extraction process, and belongs to the field of natural perfume extraction.The extraction process of the citrus essential oil comprises the following steps that S1, peel of disinfected and cleaned fresh citrus fruits is obtained; s2, sequentially crushing and squeezing the fruit peels under the assistance of ultrasonic waves or without ultrasonic waves, and then separating out solid matters to obtain virgin juice; and S3, standing and layering the virgin juice in an environment of 0-10 DEG C, then centrifuging at a controlled temperature at a centrifugal speed of 2000-60000r / min for 1-180min, and separating out upper-layer essential oil within 30min after centrifugation is finished, so as to obtain the citrus essential oil. The process can improve the essential oil yield, reduce the water content and maintain the natural fragrance close to that of fresh fruits, and can be applied to the field of food and the field of daily chemicals.