Method of stabilizing protein

US20050171339A1Inactive Publication Date: 2005-08-04CHUGAI PHARMA CO LTD
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
CHUGAI PHARMA CO LTD
Publication Date
2005-08-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present inventors revealed that deamidation of an antibody can be suppressed without influencing its activity by substituting a glycine that is located adjacent to an asparagine with another amino acid.
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Description

TECHNICAL FIELD

[0001] The present invention relates to a method for improving protein stability. Specifically, the present invention relates to a method for stabilizing proteins comprising the step of substituting the amino acid that is located adjacent to the amino acid being deamidated in a protein with another amino acid. BACKGROUND ART

[0002] Gradual deamidation of amino acids, such as asparagine, in proteins over time is mentioned as a cause of the reduction in protein stability. When proteins, particularly antibodies, are used as pharmaceutical agents for various diseases, they are required to be stable over a long period. However, the activity of antibody decreases with time. The cause for reduction in activity varies in antibodies, and deamidation of amino acids, such as asparagine, comprised in the antibody is also mentioned as one of the causes.

[0003] Therefore, proteins can be stabilized by suppressing deamidation of asparagines. Thus, research on suppressing deamidatio...

Claims

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