1498 results about "Aspartic acid" patented technology

The invention belongs to the technical field of protein engineering, and discloses a Brazilian sweet protein mutant and application thereof. According to the invention, lysine is used for replacing aspartic acid at the 29th site of Brazilian sweet protein, and / or arginine is used for replacing glutamic acid at the 36th site, and / or alanine is used for replacing aspartic acid at the 50th site, amino acid mutation at the sites is carried out to construct a Brazilian sweet protein mutant, and the Brazilian sweet protein mutant is subjected to heterologous expression in pichia pastoris GS115, so that the Brazilian sweet protein mutant is obtained. And carrying out sweetness performance evaluation on the expressed Brazilian sweet protein mutant. The sweetness property of the double mutant Asp29Lys-Asp50Ala protein is improved by 240% compared with the sweetness property of the Brazilian sweet protein before mutation.

The invention discloses a high-performance carbonyl reductase mutant and application thereof in synthesis of a series of chiral alcohols. The high-performance carbonyl reductase mutant is obtained by performing the following mutations on an amino acid sequence as shown in SEQ ID NO.2: the 69th alanine mutates into any one of lysine, leucine, aspartic acid or asparagine, and the 69th alanine mutates into any one of lysine, leucine, aspartic acid or asparagine; and / or the 100th glycine is mutated into any one of glutamic acid, serine, lysine or asparagine. Compared with a wild type enzyme, the thermal stability and the catalytic activity of the obtained mutant are remarkably improved, particularly, single point mutants Mut-A69N, Mut-G100E and Mut-G100N and a combined mutant Mut-A69N-G100N show excellent relative enzyme activity, thermal stability and substrate tolerance. The mutant has high catalytic activity and stability to various chiral alcohols, and the contradiction that the traditional carbonyl reductase is easy to inactivate at high temperature and the catalytic efficiency is difficult to achieve at the same time is solved. The invention provides an efficient and stable enzyme catalysis tool for green biological preparation of chiral drug intermediates.

The invention discloses a potassium fulvate chelated macroelement water-soluble fertilizer with stress resistance and growth promotion functions, and relates to the technical field of water-soluble fertilizers, the potassium fulvate chelated macroelement water-soluble fertilizer comprises the following raw materials by weight: 167-210 parts of mineral source potassium fulvate, 450-460 parts of potassium nitrate, 300-310 parts of urea, 150-160 parts of potassium dihydrogen phosphate, 30-40 parts of potassium sulfate, 15-25 parts of EDTA-disodium, and 5-15 parts of citric acid. 0.5 to 1.5 parts of brassinolide and 4 to 6 parts of polyaspartic acid; according to the potassium fulvate chelated macroelement water-soluble fertilizer with the stress-resistant and growth-promoting functions, through chelation cooperation of potassium fulvate and nitrogen, phosphorus and potassium, the solubility of the chelated phosphorus element in water is increased, and the chelated phosphorus element can be more uniformly distributed in soil; by adopting an EDTA-disodium and citric acid composite chelating system, volatilization and loss of nitrogen elements are reduced, the problem of precipitation of calcium, magnesium and phosphorus is solved, and the soil conditioner is suitable for hard water areas and especially has outstanding performance in saline-alkali soil and arid areas.

The invention relates to a concrete anti-cracking self-repairing agent in the technical field of building materials, and aims to solve the problem that concrete cracks are difficult to self-repair. According to the technical scheme, the self-repairing agent is characterized by comprising a microbial repairing unit formed by ZSM-5 type zeolite molecular sieve loaded bacillus pasteurii spores, mesoporous silica particles loaded with L-calcium aspartate and Fe3O4 (at) SiO2 core-shell nanoparticles which are fixed through an in-situ polymerization coating layer to form a magnetic thermal response capsule layer coating the surface of the microbial repairing unit, pLGA microspheres containing yeast extract are positioned on an interface of zeolite and hydrogel, and a trehalose-anhydrous calcium chloride eutectic compound is used as a directional mineralization accelerant. The concrete anti-cracking self-repairing agent provided by the invention is mainly used for intelligent identification and self-repairing of concrete cracks, and efficient repairing is realized through a microbial mineralization, calcium ion slow release and magnetic thermal response synergistic mechanism.

The invention relates to hydrogel loaded with oxygen self-producing nanoparticles as well as a preparation method and application of the hydrogel. The preparation method comprises the following steps: 1, taking cross-linked methacrylamide silk fibroin and hyaluronic acid modified by arginine-glycine-aspartic acid peptide as base materials, and loading self-oxygen-producing nano particles CaO2 (at) PVP (at) Cu-EGCG, and the self-oxygen-producing nano particles CaO2 (at) PVP (at) Cu-EGCG are prepared by coating Cu-EGCG on calcium peroxide nano particles modified by PVP modified polyvinylpyrrolidone. The hydrogel can improve energy metabolism and regulate immune environment through dual mechanisms, realizes efficient promotion of myocardial repair, and provides a new strategy and thought for tissue regeneration and functional recovery after myocardial infarction.

The invention relates to the technical field of microbial genetic engineering, in particular to a method for improving cordycepin synthesized by saccharomyces cerevisiae and saccharomyces cerevisiae engineering bacteria. According to the invention, codon-optimized 2 '-carbonyl-3'-deoxyadenosine reductase gene and 3 '-adenosine monophosphate phosphohydrolase gene from cordyceps militaris are expressed by using plasmids, and then the plasmids are transformed into host cells to obtain the saccharomyces cerevisiae engineering bacteria. And inoculating a seed solution obtained by seed culture of the activated saccharomyces cerevisiae engineering bacteria into a fermentation culture medium containing a metabolic effector, and carrying out fermentation culture to synthesize cordycepin. Metabolic effectors (Cu < 2 + >, Fe < 2 + >, Mg < 2 + >, Zn < 2 + >, citric acid, cysteine, aspartic acid, glycine, VB1 and tea polyphenol) are added into a fermentation culture medium strain, and the variety and concentration of the metabolic effectors are optimized, so that the yield and synthesis efficiency of cordycepin are remarkably improved. By using the combination of the strain obtained by the invention and the optimal effector, the yield of cordycepin in a fermentation tank reaches 2.9 g / L.

The invention discloses a cyclic nonapeptide with repairing and anti-oxidation effects and application of the cyclic nonapeptide, the amino acid sequence of the cyclic nonapeptide is cyclic (arginine-glycine-aspartic acid-serine-arginine-lysine-valine-lysine-serine), the prepared cyclic nonapeptide has the repairing and anti-oxidation effects, and the cyclic nonapeptide has the repairing and anti-oxidation effects. The composition can be applied to preparation of cosmetics with repairing and / or anti-oxidation effects.

The invention discloses an efficient wet desulfurization synergist and a preparation method thereof, and relates to the technical field of flue gas desulfurization. The efficient wet desulphurization synergist comprises the following raw materials in parts by weight: 10-20 parts of sodium formate, 5-10 parts of citric acid, 20-30 parts of binary acid, 5-6 parts of a surfactant, 15-20 parts of magnesium salt, 12-18 parts of sodium salt and 2-5 parts of a pH buffer agent. The preparation method comprises the following steps: reacting cyanuric chloride with lauryl amine to generate a monosubstituted compound; then reacting with L-asparaginic acid to generate a disubstituted compound; reacting with diethylenetriamine to generate a bis-triazine ring compound; and finally, reacting with sodium 2-chloroethanesulfonate to generate the surfactant. The high-efficiency wet desulphurization synergist prepared by the invention has a desulphurization effect.

The invention relates to laccase as well as a mutant and application thereof, and belongs to the technical field of biology. The amino acid sequence of the laccase PnLac1 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the laccase PnLac1 is as shown in SEQ ID NO. 1. The mutant is characterized in that aspartic acid at the 166th position of laccase PnLac1 is subjected to site-specific mutagenesis, histidine at the 348th position of laccase PnLac1 is subjected to site-specific mutagenesis, methionine at the 403th position of laccase PnLac1 is subjected to site-specific mutagenesis, or leucine at the 467th position of laccase PnLac1 is subjected to site-specific mutagenesis, amino acid sequences are shown as SEQ ID NO.4, SEQ ID NO.6, SEQ ID NO.8 and SEQ ID NO.10, and nucleotide sequences are shown as SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7 and SEQ ID NO.9. The decoloring efficiency of the laccase PnLac1 on crystal violet reaches 5h, and the degradation rate reaches 75%. When the ABTS is used as a substrate, the enzyme activities of the laccase mutants H348F and L467M are respectively improved by 1.89 times and 1.65 times, and the ABTS has the potential of degrading stubborn substances; when guaiacol is used as a substrate, the enzyme activities of the laccase mutants D166A and M403F are respectively improved by 2.07 times and 3.72 times, and the guaiacol has a relatively high application prospect in the field of lignin.

The invention relates to a phosphorus-free zinc-free seawater special-purpose scale and corrosion inhibitor, which comprises, by weight, 20-25% of sulfonic acid modified polyaspartic acid, 8-12% of quaternized chitosan, 5-10% of a sulfonate copolymer, 5-10% of polyepoxysuccinic acid, 1-3% of benzotriazole or methyl benzotriazole, 0.5-1% of sodium tungstate, and the balance of deionized water. According to the formula, traditional corrosion inhibitors such as phosphorus and zinc are completely abandoned, functions are achieved through natural polymer derivatives such as sulfonic acid modified polyaspartic acid and quaternized chitosan, and the scale and corrosion inhibitor has the following environmental protection advantages that the scale and corrosion inhibitor does not contain phosphorus and zinc elements at all, and water eutrophication and heavy metal accumulation caused by a traditional phosphorus and zinc formula are avoided.

The invention relates to a composite reverse osmosis membrane acidic cleaning agent, a preparation method and a cleaning process thereof, and belongs to the technical field of environment-friendly water treatment. The cleaning agent comprises the following components in percentage by mass: 4-8% of inorganic acid, 20-40% of organic acid, 1-3% of a surfactant, 0.5-1% of a chelating agent, 0.5-1% of a compound corrosion inhibitor and the balance of deionized water. The composite corrosion inhibitor is formed by compounding 2-mercaptobenzothiazole and the like according to a specific mass ratio, the molecular weight of polyaspartic acid is 5000, and the molar ratio of Zn to Mo in the zinc-molybdate compound is 2: 1. The preparation method comprises the following four steps: firstly preparing a compound corrosion inhibitor premix, then preparing an acidic base solution, and then sequentially adding the components to finally obtain the cleaning solution. According to the cleaning process, a cleaning agent and deionized water are mixed, the membrane flux recovery rate is larger than or equal to 85% through cleaning of a specific process, the reverse osmosis membrane can be effectively cleaned, and the performance of the reverse osmosis membrane can be recovered.

The invention provides a fertilizer synergist for reducing nutrient loss and inhibiting nitrification and application of the fertilizer synergist. The fertilizer synergist is prepared from the following components in percentage by mass: 95 to 98 percent of carboxyl cross-linked modified montmorillonite, 0.05 to 0.1 percent of mannan oligosaccharide, 0.1 to 0.5 percent of mannan, 1 to 3 percent of polyaspartate and 0.5 to 2 percent of a nitrification inhibiting aid. According to the fertilizer synergist capable of reducing nutrient loss and inhibiting nitrification and the application of the fertilizer synergist, the fertilizer synergist serves as a plant nutrient loss control synergist and a nitrification inhibitor and can activate a crop stress resistance signal source, promote and induce synthesis of endogenous auxin in plants, root and growth, activate phosphorus and dissolve potassium, reduce nitrogen loss and improve the yield of the plants. The fertilizer utilization rate is improved.

The invention provides a culture medium formula capable of effectively improving vibrio inhibition capability of bacillus. The culture medium formula comprises 10-20 g of corn starch, 2-10 g of yeast powder, 2-10 g of malt extract, 5-20 g of glycerin, 0.5-1 g of L-aspartic acid, 1 g of dipotassium phosphate, 0.3 g of monopotassium phosphate, 0.5 g of magnesium sulfate, 1 g of calcium carbonate and 1000 mL of distilled water. When the culture medium is used for culturing bacillus capable of naturally inhibiting vibrio, the obtained culture has a better vibrio inhibiting effect.

The invention discloses a growth regulator for promoting colonization of plant rhizosphere microorganisms and application of the growth regulator to crops, a preparation method of the growth regulator comprises the following steps: S1, dropwise adding an aqueous solution of modified chitosan and a compound bacteria solution into a saturated boric acid solution of 1-2% calcium chloride, crosslinking and curing for 3-4 hours, washing with sterile water for 3-5 times, and drying to obtain the growth regulator for promoting colonization of the plant rhizosphere microorganisms; immobilized microorganisms are obtained; s2, sequentially adding nanocellulose crystals, sodium polyaspartate and organic fertilizer into the enteromorpha extracting solution, uniformly stirring and mixing, adding immobilized microorganisms, uniformly mixing, granulating, coating with modified chitosan, and drying to obtain the growth regulator. Through step-by-step design of microorganism immobilization, nutrition compounding and chitosan coating, function integration of microorganism protection, nutrition release and environmental response is achieved, the colonization efficiency of rhizosphere microorganisms is remarkably improved, the soil microenvironment is improved, and finally comprehensive improvement of plant growth and stress resistance is achieved; and the method has relatively high social use value and application prospect.

The invention discloses an L-aspartic acid transaminase mutant and application thereof. The L-aspartic acid transaminase mutant is obtained by performing single mutation on the 104th amino acid of the amino acid sequence of L-aspartic acid transaminase. The L-aspartic acid transaminase mutant is used for synthesizing an L-phenylglycine derivative. The L-aspartic acid transaminase mutant is from escherichia coli, homologous sequence analysis and molecular docking simulation are carried out, site-specific mutagenesis verification is combined, and single-point mutation is carried out on the 104 amino acid to obtain the T104S mutant. The L-aspartic acid transaminase mutant disclosed by the invention can be used for efficiently catalyzing to generate an L-phenylglycine derivative, and compared with a wild type, the specific activity of the T104S mutant to o-chlorophenoxyacetic acid is 3.2 times that of the wild type.

The invention relates to the technical field of biology, and discloses a carbonyl reductase mutant and application thereof in enzymatic synthesis of chiral alcohol. The amino acid sequence as shown in SEQ ID NO.2 is subjected to mutation that 36th lysine is mutated into any one of aspartic acid, threonine and valine, or / and 125th histidine is mutated into any one of lysine, serine and leucine, or 170th histidine is mutated into any one of lysine, cysteine and leucine. The carbonyl reductase mutants, especially single-site mutants Mut-T75K, Mut-T75D and Mut-E216M and a combined mutant Mut-T75K-E216M, with activity improved to different extents compared with that of a wild type are obtained, have excellent relative enzyme activity, thermal stability and substrate tolerance, have relatively high activity and stability for various chiral alcohols, and can be used for preparing the carbonyl reductase mutants. The catalyst has the advantages of high catalytic activity, high thermal stability, strong substrate tolerance, easiness in fermentation and the like.

The invention relates to the technical field of scale and corrosion inhibitors, in particular to a preparation method and application of an enhanced boric acid efficient environment-friendly phosphorus-free scale and corrosion inhibitor, and the enhanced boric acid efficient environment-friendly phosphorus-free scale and corrosion inhibitor comprises the following components in parts by weight: 50-100 parts of a water-soluble polyol-boric acid mixture, 0-50 parts of polyepoxysuccinic acid, 0-50 parts of polyaspartic acid, 0-50 parts of benzotriazole and 0-50 parts of zinc chloride. The high intermiscibility of polyhydric alcohol, water and boric acid is utilized, the dosage of boric acid in a solution is effectively increased, the effects of boric acid on scale inhibition, corrosion inhibition, sterilization and the like are amplified, meanwhile, the freezing temperature of the scale and corrosion inhibitor is reduced due to the addition of polyhydric alcohol, the scale and corrosion inhibitor can be used in winter more easily, and the scale and corrosion inhibitor is suitable for popularization and application. The invention provides an implementation way of a novel efficient environment-friendly phosphorus-free scale and corrosion inhibitor, and the scale and corrosion inhibitor has the advantages of environment friendliness, convenience in use and good scale and corrosion inhibition performance.

The invention relates to the technical field of biology, in particular to a gene for increasing the content of resveratrol in grapes and application of the gene. The invention discloses a gene for improving the content of resveratrol in grapes. The DNA sequence of the gene is shown as SEQ ID NO1. The amino acid sequence of the gene synthetic protein disclosed by the invention is as shown in SEQ ID NO2, and the protein is named as VvFHY34D. According to the invention, when four amino acids S124, T128, S156 and T160 of the VvFHY3 are all replaced with aspartic acid to simulate phosphorylation activation, the VvSTS expression quantity in transient over-expression VvFHY34D grapes is higher, and more resveratrol can be synthesized.

The invention discloses a p-nitrophenyl ethyl esterase mutant with high enantioselectivity as well as a construction method and application of the p-nitrophenyl ethyl esterase mutant. The p-nitrophenyl ethyl esterase mutant is obtained by mutating an amino acid sequence of wild p-nitrophenyl ethyl esterase pnbA as shown in SEQ ID NO.1 according to one or a combination of more of the following modes: leucine at the 273rd site is mutated into aspartic acid, phenylalanine at the 314th site is mutated into histidine, and leucine at the 362nd site is mutated into arginine. The mutant pnbA-L273D / F314H / L362R provided by the invention shows optimal catalytic performance (E = 47.16) for racemization-acetic acid pinyl hydrate, the enantiomeric excess value of the mutant is 95.13%, the conversion rate of 1S, 5R-acetic acid pinyl hydrate is 90.42%, the enantioselectivity of the mutant is obviously higher than that of a wild type, and the mutant has great application potential in biological catalytic synthesis of monocyclic monoterpenoid chiral alcohols.

The invention relates to a corrosion and scale inhibitor for TRT and a preparation method of the corrosion and scale inhibitor, and relates to the technical field of chemical reagents. The corrosion and scale inhibitor is prepared from the following components in percentage by mass: 15 to 20 percent of morpholine, 25 to 30 percent of aminoethyl ethanolamine, 15 to 20 percent of 1-hydroxyethyl-2-undecylimidazoline onium salt, 12 to 18 percent of sodium polyaspartate, 8 to 12 percent of itaconic acid-allylsulfonic acid copolymer, 1 to 3 percent of alkyl glycoside and the balance of a mixture of water and glycerol in a mass ratio of 1 to (1 to 3). The corrosion and scale inhibitor disclosed by the invention can synchronously inhibit corrosion of an acid medium and scale deposition of salts such as NH4Cl and the like, and the corrosion inhibition rate and the scale inhibition rate are effectively improved.

A boron carrying agent, a preparation method therefor, and a pharmaceutical formulation. The boron carrying agent contains compound 1, N-4-carboxyphenylboronic acid amide-threonine-phenylalanine-phenylalanine-tyrosine-glycine-glycine-serine-arginine-glycine-lysine-arginine-aspartic acid-aspartic acid-phenylalanine-lysine-threonine-glutamic acid-glutamic acid-tyrosine-cysteine, having a molecular formula of C114H158BN30O35S1 and a molecular weight of 2552.4, and further contains compound 2, N-4-carboxyphenylboronic acid amide-arginine-lysine-lysine-lysine-arginine-arginine-glutamine-arginine-arginine-arginine, and / or compound 3, N-4-carboxyphenylboronic acid amide-methionine-alanine-serine-methionine-threonine-glycine-glycine-glutamine-glutamine-methionine-glycine. Compounds 1-3 can serve as a targeted medicament for boron neutron capture therapy used for treating a cerebral glioma and a head and neck tumor.

The art does not provide systematic and reproducible methods to personalize dosing of multiple immunosuppressive medications after transplantation. This invention provides a method to systematize multi-drug immuno suppression management in tissue and organ transplantation using an artificial intelligence-based complex systems approach. In embodiments of this invention, immunosuppression drug dose, blood drug concentrations, donor-derived fraction of cell free DNA (dd-cfDNA %), and aspartate aminotransferase are used to indicate allograft status or a proxy for allograft status, to generate a phenotypic response surface to produce individual treatment modalities and dosages using empirically determined unique coefficients. This surface is used to calculate appropriate immunosuppression drug doses associated with the desired outcome for that patient. Embodiments of this disclosure are directed to identifying optimized combinations of inputs for the complex system of the immunosuppressed transplant patient in order to avoid transplant rejection while avoiding unnecessary toxicity and maintaining a robust enough immune response to fight infection.

The invention provides a lysine artificial operon and application thereof. The gene sequence of the artificial operon comprises a promoter, a lysC gene for coding aspartate kinase, an asd gene for coding aspartate semialdehyde dehydrogenase, a dapA gene for coding dihydropicolinic acid synthetase and a dapB gene for coding dihydropicolinic acid reductase, the ddh gene is used for coding diaminopimelate dehydrogenase; the lysA gene is used for coding diaminopimelate decarboxylase; except for the first lysC gene, sequences of RBS ribose binding sites of other genes are RBS ribose binding sites translated after gene transcription by intercepting 20-26nt DNA sequences at the upstream of a coding region ATG (adenosine triphosphate); the RBS ribose binding site is connected in front of the corresponding gene.

The invention provides a green compound scale inhibitor for geothermal fluid. The compound scale inhibitor comprises modified polyaspartic acid, high-hydrolysis polymaleic anhydride, polyepoxysuccinic acid and polyacrylic acid. According to the environment-friendly compound scale inhibitor for the geothermal fluid, the modified polyaspartic acid, the high-hydrolysis polymaleic anhydride, the polyepoxysuccinic acid and the polyacrylic acid are compounded, a good synergistic effect is achieved, scale inhibition is achieved through chelation solubilization and lattice distortion, the scale inhibition advantage in actual geothermal water is obvious, and the environment-friendly compound scale inhibitor has a good actual popularization value.

The invention belongs to the technical field of bioengineering, and relates to a transaminase mutant, a recombinant genetically engineered bacterium and application of the transaminase mutant in catalytic synthesis of (R)-1-Boc-3-aminopiperidine.The transaminase mutant is obtained by conducting single-point or combined mutation on the 131 site and / or the 197 site of an amino acid sequence shown in SEQ ID NO.2; the mutation sites comprise that the 131 phenylalanine is mutated into aspartic acid, threonine or tyrosine, and / or the 197 lysine is mutated into arginine or leucine. Experimental results show that compared with wild type transaminase, the catalytic activity, the thermal stability and the organic solvent tolerance of the obtained mutants, especially single-point mutants MyTA1-F131Y and MyTA1-K197R and a combined mutant MyTA1-F131Y-K197R, are all remarkably improved, and compared with the wild type transaminase, the catalytic activity, the thermal stability and the organic solvent tolerance of the obtained mutants are all remarkably improved. The mutant MyTA1-F131Y-K197R can be used for efficiently catalyzing asymmetric amination of N-Boc-3-piperidone to synthesize (R)-1-Boc-3-aminopiperidine, the conversion rate of the (R)-1-Boc-3-aminopiperidine after the (R)-1-Boc-3-aminopiperidine reacts for 24 hours under the condition that the substrate concentration is 100 g / L can reach 90% or above, and the mutant MyTA1-F131Y-K197R has a good industrial application prospect.

The invention belongs to the technical field of comprehensive utilization of rare earth resources, and particularly relates to a combined inhibitor and a calcium-containing rare earth ore flotation method thereof. The combined inhibitor is a combination of an organic chelating agent aqueous solution and a water glass aqueous solution; the organic chelating agent is one of polyaspartic acid, hydrolytic polymaleic acid and carboxymethyl chitosan; the mass ratio of the organic chelating agent to the water glass is 2: (10-25); the mass percent of the organic chelating agent aqueous solution is 2-10%, and the mass percent of the water glass aqueous solution is 5-8%. The combined inhibitor has the advantages of being high in selective inhibition effect on calcium-containing gangue minerals, environmentally friendly, environmentally friendly, easy to degrade, low in reagent dosage and the like, the grade and the recovery rate of rare earth in obtained rare earth ore concentrate are high, and the process structure is simple.

The invention belongs to the field of preparation of soil conditioners, and particularly relates to a soil loosening and root promoting soil conditioner and a preparation method thereof. A polymer formed by vinyltriethoxysilane, polyacrylic acid and coumarin under the catalytic action of citric acid is mixed with porous powder obtained after crushing and nutrient organic matter, and freeze-drying is performed to obtain the soil-loosening improved fermentation substrate which can be slowly released in soil; polyaspartic acid and acetylchitosamine are cross-linked under the catalytic action of N-hydroxysuccinimide, root-promoting nutrition powder and auxiliary root-promoting components are mixed, and the obtained composite root-promoting agent can significantly promote the growth of plant root systems; a porous structure contained in the protective stability maintaining agent can provide a relatively stable microenvironment for effective components, and the soil loosening and root promoting agent is mixed with the soil loosening and improving fermentation substrate and the composite root promoting agent, so that the prepared soil loosening and root promoting soil conditioner is remarkable in soil loosening and root promoting effect, long-acting and stable, small in dosage, obvious in effect and stable in conditioning effect after being stored.

The invention relates to a mutant based on cytochrome P450BM3 and application of the mutant in chloroaniline biodegradation, and belongs to the field of enzymology. The mutant is obtained by mutating threonine at the 268 site of an amino acid sequence shown in SEQ ID NO.1 into aspartic acid or glutamic acid, mutating alanine at the 328 site into phenylalanine, and mutating alanine at the 328 site into phenylalanine. According to the present invention, the amino acid at the 87 site is mutated into alanine, and the valine at the 78 site is mutated into alanine, such that a variety of mutants such as T268D / A328F, T268E / A328F, T268D / A328F / F87A, T268E / A328F / F87A, T268D / A328F / F87A / V78A and T268E / A328F / F87A / V78A are obtained; according to the present invention, the degradation rate of the mutant on 4-chloroaniline, 2-chloroaniline, 2, 4-dichloroaniline, 3, 4-dichloroaniline and 3, 5-dichloroaniline is achieved; the method has important significance and application value for promoting rapid degradation of chloroaniline pollutants in a water environment and maintaining ecological balance.

The invention provides a method for detecting free amino acids in camellia oleosa seed oil, and establishes a method for detecting the free amino acids in the camellia oleosa seed oil based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), which comprises the following steps: pre-treating various amino acids in the camellia oleosa seed oil by using a non-derivation method; the problems of complicated operation, impurity interference and the like caused by derivatization are avoided, the extraction solvent is environment-friendly and economical, the extraction efficiency is high, and the pretreatment time is greatly shortened; then selecting a proper chromatographic column and a mobile phase, separating a plurality of free amino acids under the condition of not introducing excessive derivatization agents and ion pair reagents, and controlling the collection time to be 12 minutes. The method for detecting the free amino acids in the camellia oleosa seed oil has the characteristics of no need of derivatization treatment, simplicity and convenience in operation, high detection efficiency and good sensitivity and accuracy, and can effectively separate and detect 14 free amino acids such as alanine, phenylalanine and aspartic acid in the camellia oleosa seed oil.

This disclosure describes methods for improving umami and meaty flavors of cell-based food products. In particular, the disclosed method includes an amino acid integration method where cultured cells and exogenous amino acids (e.g., glutamic acid and aspartic acid) are combined and heated, resulting in a synergistic improvement of umami flavors. The disclosed method further includes a browning method by which a cell suspension is dehydrated and heated at high temperatures (e.g., greater than 140C) to induce non-enzymatic browning and caramelization reactions in the cell suspension. The cell-based flavoring product can be combined with a food product as a flavor, aroma, and color enhancer. The disclosed methods may use one or both of the amino acid integration method and the browning method to improve organoleptic properties of cell-based food products.