Transgenic plants that exhibit enhanced nitrogen assimilation

a technology of enhanced nitrogen assimilation and transgenic plants, applied in the field of gene engineering plants, can solve the problems of unable often limiting nitrogen, and failure to report any positive effect of overexpression on plant growth, etc., to achieve enhanced growth characteristics, enhanced nitrogen assimilation or utilization enzymes, and enhanced growth characteristics

a technology of enhanced nitrogen assimilation and transgenic plants, applied in the field of gene engineering plants, can solve the problems of unable often limiting nitrogen, and failure to report any positive effect of overexpression on plant growth, etc., to achieve enhanced growth characteristics, enhanced nitrogen assimilation or utilization enzymes, and enhanced growth characteristics

US20020069430A1Inactive Publication Date: 2002-06-06AJINOMOTO CO INC

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  • Transgenic plants that exhibit enhanced nitrogen assimilation
  • Transgenic plants that exhibit enhanced nitrogen assimilation
  • Transgenic plants that exhibit enhanced nitrogen assimilation

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Embodiment Construction

[0065] The present invention relates to genetic engineering of nitrogen metabolism in plants. In particular, the invention relates to altering the enzymes involved in nitrogen assimilation or utilization and / or their expression in order to engineer plants with better growth characteristics, enriched nutritional qualities, improved vegetative and yield and / or enhanced seed yield or quality.

[0066] Accordingly--without intending to be limited to a particular mechanism--the targets for engineering are genes encoding for enzymes involved in the assimilation of ammonia into the amino acids, glutamine, aspartate, asparagine or glutamate, or in the utilization of these same amino acids in biosynthetic reactions. The target genes include those encoding glutamine synthetase (GS), asparagine synthetase (AS), glutamate 2:oxoglutarate -aminotransferase (GOGAT), aspartate aminotransferase (AspAT), glutamate dehydrogenase (GDH) and asparaginase -(ANS). See FIG. 1 for a diagram of the roles played ...

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Abstract

Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate dehydrogenase (GDH) gene is introduced into a plant together with a regulator sequence suitable for over expressing the sequence encoding GDH gene in plant cells.

Description

[0001] This application is a continuation-in-part of co-pending application Ser. No. 08 / 132,334 filed Oct. 6, 1993, which is hereby incorporated by reference in its entirety.1. INTRODUCTION[0003] The present invention relates generally to genetic-engineering plants to display enhanced nitrogen assimilatory and utilization capacities, grow larger, more efficiently or rapidly, and / or have enriched nitrogen contents in vegetative and / or reproductive plant parts and / or increased biomass. More particularly, this invention relates to producing transgenic plants engineered to have altered expression of key enzymes in the nitrogen assimilation and utilization pathways. The engineered plants may be productively cultivated under conditions of low nitrogen fertilizer input or in nitrogen poor soils. Alternatively, the engineered plants may be used to achieve faster growing or maturing crops, higher crop yields and / or more nutritious products under ideal cultivation conditions.2. BACKGROUND OF ...

Claims

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Application Information

Patent Timeline
06 Jun 2002
Publication
US20020069430A1
IPC
C12N9/00; C12N9/02; C12N15/53; C12N15/82; C12N15/83
CPC
C12N9/0004; C12N9/0016; C12N9/93; C12Y104/01004; C12N15/8251; C12N15/8261; C12Y104/01002; C12N15/8241
Inventors
KISAKA, HIROAKI; KIDA, TAKAO