137results about How to "Improve growth characteristics" patented technology

Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate dehydrogenase (GDH) gene is introduced into a plant together with a regulator sequence suitable for over expressing the sequence encoding GDH gene in plant cells.

Methods and associated apparatus for automatically growing agricultural crops vertically and / or in a continuous fashion throughout each year (Vertical Farm) in combination with contiguous and co-located production of biofuel, food, biomass for the purpose of carbon sequestering (carbon credits), and biomass electric power generation. A process that incorporates vast arrays of continuous-loop conveyors, towering upon vertical framework, which allow potted plants to be transported throughout all stages of maturity in a manner which substantially multiplies yield per acre, allows production to proceed in both natural and artificial light, allows production and harvesting to be automated, and allows production to proceed in conditions which are highly favorable to plants but unfavorable to humans. The entire apparatus can be constructed of lightweight, cost-effective materials which afford mass-production and mass-array into vast automatic growing operations.

A method is provided for raising poultry, such as chickens, for food production. In a first preferred embodiment, the method includes the steps of: providing a facility for housing the poultry, providing at least one light-absorbing ventilation fan associated with the facility for ventilating the facility, exposing an interior of the facility to natural light cycles of an outside environment for a first period and regulating light cycles of the interior for a second period, thereby mimicking daylight duration variation representative of seasonal changes for stimulating sexual development of the poultry. In a second preferred embodiment, the method includes the steps of: providing a facility for housing the poultry, providing at least one light-absorbing ventilation fan associated with the facility for ventilating the facility, limiting exposure of an interior of the facility to light to produce a brown-out effect therein for enhancing physical development of the poultry.

The invention discloses a method for sequential culture of human umbilical cord blood mesenchymal stem cells by using two culture media. A lymphocyte separation medium density gradient centrifugation method is used for separating umbilical cord blood mononuclear cells, a dulbecco modified eagle medium (DMEM) / F12 is used for primary culture of the mesenchymal stem cells of the human umbilical cord blood, and the method increases adherence of the cells, reduces growth of osteoclast like cells remarkably, facilitates formation of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) colonies, and greatly improves successful rate of culture of the hUCB-MSCs. The DMEM / F12 is used continuously for subculturing to a P2 generation, the method is combined by a using enzymatic digestion and differential velocity adherent method simultaneously, and the method facilitates purification of P1-P2 generation cells remarkably. A P3 generation and the following generations are cultured by using an Oricell human umbilical cord mesenchymal stem cell culture medium, marker proteins and good morphological characteristics and growth characteristics of the hUCB-MSCs are maintained, and multilineage differentiation potential of the hUCB-MSCs is maintained. In addition, costs of the culture media adopted by the method are reduced remarkably.

The invention relates to a special rhodococcus ruber (CGMCC NO. 17012) fermentation method, a preparation process of adjuvants with different dosage forms and an application as an adjuvant in animalvaccines. Rhodococcus ruber strains are separated from a farm and obtained through single colony cloning purification and identification, the invention provides a special fermentation process where the strain inactivation product is used as an animal vaccine adjuvant, and provides a preparation process of different dosage forms of adjuvants containing the strain and the product. Animal experimentsprove that when the adjuvant product is used for univalent or multivalent animal vaccines, particularly Newcastle disease inactivated vaccine, avian influenza inactivated vaccine and swine fever livevaccine, definite non-specific immune enhancement effect can be provided, specifically, the antibody peak value level induced by the animal vaccine is obviously improved, the time for producing the protective antibody level is advanced, the antibody maintenance period is prolonged, and the immune effect of the animal vaccines is enhanced.

The invention discloses a drug substrate fertilizer particle agent to prevent cotton seeding pest, which is characterized by the following: adopting nitrogen, phosphor, potassium or composite fertilizer as carrier; allocating imidacloprid, thiram, triazole ketone and adjuvant according to certain proportion; making the drug particle agent; modifying the nourishing condition and seeding growing environment; reinforcing pest-proof and growing property.

The invention discloses a high-nutrient beef cattle feed capable of promoting nutrition absorption and utilization and increasing the growth speed and a preparation method of the high-nutrient beef cattle feed. The feed is capable of promoting absorption and utilization of beef cattle on nutrient substances, increasing the growth speed of the beef cattle, promoting protein synthesis and accumulation and improving the health of the intestinal environment of the beef cattle. Growth-weight gain of the beef cattle is accelerated under cultivation of the feed; the cultivation period is shortened; the meat factor is improved; the meat quality becomes good; and the feed utilization rate is improved. The high-nutrient beef cattle feed has important significance in acceleration of development of current beef cattle cultivation.

A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and / or mutated ptr gene and / or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.

The invention discloses a doping gadolinium yttrium scandium gallium garnet, a gadolinium yttrium scandium gallium aluminium garnet and a method of the fused mass normal crystal for preparing the gadolinium yttrium scandium gallium garnet and the gadolinium yttrium scandium gallium aluminium garnet crystal or other corresponding compound for preparing; mixing the prepared raw material fully and pressing forming, and becoming initial raw material for crystal growth after high temperature calcination or non high temperature calcination; adding the growth initial raw material into a pot for heating fully to melt and becoming an initial melt of crystal growth with melt method, the obtained crystal can be used as a solid laser working substance.

The invention relates to a Newcastle disease chimeric virus marker vaccine strain as well as a construction method and application thereof, and belongs to the field of rescue and application of Newcastle disease chimeric vaccine strains. A Newcastle disease virus reverse genetic operation platform is utilized to mutate an F protein cleavage site of a Newcastle disease gene GVII type strain into acleavage site of an attenuated strain, F and HN genes of a mutated gene VII type Newcastle disease strain and NP, P, M and L of a gene II type NDV La Sota strain construct a full-length chimeric cDNAsequence, and a 18bp nucleotide marker sequence is inserted into a non-coding region between P and M. A Newcastle disease chimeric virus NDV DC strain is obtained through transfection cell rescue. Theconstructed chimeric virus can reach relatively high culture titer in chick embryos and cells. The chimeric strain contains envelope surface glycoprotein of the gene VII type Newcastle disease strain, contains a skeleton of the gene II type strain, and has immunogenicity of the Newcastle disease gene VII type strain and high reproduction and high safety characteristics of the gene II type La Sotastrain.

A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and / or mutated ptr gene and / or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.

The invention discloses preparation and application of an alpha-1,6 mannose transferase gene (och1) flaw type P. pastoris X-33 bacterial strain, belonging to the technical field of biological engineering. The engineering bacterial strain P. pastoris X-33 (delta och1) is characterized in that a target gene knockout method by double-exchange homologous recombination is adopted, and a URA3 gene serves as a selective marker to knock out the alpha-1,6 mannose transferase gene (och1) of the P. pastoris X-33 (delta och1) to obtain the P. pastoris X-33 (delta och1) bacterial strain with OCH1 gene knockout. The bacterial strain provides a P. pastoris expression system which modifies protein by virtue of low N-glycosylation modification and provides a favourable basis for further glycosylation modification. The bacterial strain has the advantage of great potential application prospect.

A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and / or mutated ptr gene and / or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.

The invention discloses a method for selecting and breeding improved variety types of walnuts and belongs to the technical field of agriculture and biology. The method comprises steps of rootstock seedling selecting and breeding, scion collecting and processing, grafting, transplanting to land. The scions are selected from superior mother trees in native soil. The improved variety types of walnuts selected and bred after inlay-bark-tongue grafting has following advantages: good yield ability, large size, good appearance and early ripeness. The walnuts grow well especially in dry conditions of hilly areas, show properties of good drought tolerance, cold tolerance and disease resistance, and are suitable for promoting and planting in similar area. Green fruits of the improved variety types of walnuts are suitable for sale. The thickness of shells is suitable for machining. The walnuts are storable and transportable. The method promotes the development of walnut industry and increases the improved variety degree of walnut production.

The invention belongs to the technical field of plant tissue culture and discloses a dendrobenthemia japonica tissue culture method. The dendrobenthemia japonica tissue culture method comprises the steps of pretreating, performing primary culture, enrichment culture and strong seedling culture, planting in a rooting medium and transplanting, wherein in the culturing process, the external conditions are as follows: pH is 5.8-6.0, the culture temperature is 23-25 DEG C, the illumination period is 8-10h / d, and the illumination intensity is 1800-2000lux. The culture method has the benefits that 1. with the adoption of the culture method, a growth coefficient is high, a rooting rate is high, and a cultured plant has excellent growth characteristic; and 2. large scale production can be achieved by the culture method, and the reproduction efficiency is high.

The invention relates to a recombinant Newcastle disease virus vector novel coronavirus vaccine candidate strain as well as a construction method and application thereof, and belongs to the technicalfield of genetic engineering vaccines. According to the vaccine candidate strain disclosed by the invention, a Newcastle disease virus LaSota strain is used as a carrier, and a mutated novel coronavirus S gene (C3756T, BamHI site is removed) is inserted between a P gene and an M gene of the Newcastle disease virus LaSota strain; and the nucleotide sequence of the mutated novel coronavirus S gene is shown as SEQ ID NO. 1. The recombinant Newcastle disease virus vector novel coronavirus vaccine candidate strain provided by the invention is safe, effective and low in production cost, can stimulate an organism to generate mucosal immunity through nasal cavity inoculation, and has important application value and outstanding public health safety significance.

A fertilizer product for delivering starter fertilizer to crops in soils as an alternative to traditional phosphorus applications to enhance rapid early plant development. Specifically, the fertilizer product comprises a synergistic mixture of:from about 0.5 to about 1 part Mg: from about 2.4 to about 2.8 parts P2O5: from about 1.4 to about 2.8 parts Zn.

The invention provides a vaccine composition for infectious rhinitis of chicken. The vaccine composition comprises an immunizing dose of Avibacterium paragallinarum type A antigen and / or Avibacterium paragallinarum type C antigen and a carrier. The invention also provides a preparation method and application of the vaccine composition. The immune effect of the vaccine composition provided by the invention is superior to the immune effect of vaccines prepared from conventional strains; the content of the antigen in the vaccine composition provided by the invention can be substantially decreased, and the vaccine composition still has good immune effect; so production cost is greatly reduced.

The present invention concerns a method for improving the growth characteristics of plants by increasing expression of at least part of a Leucine Rich Repeat Receptor-Like Kinase (RKS11, RKS4 or an orthologue of these). One such method comprises introducing into a plant a nucleic acid encoding at least part of a Leucine Rich Repeat Receptor-Like Kinase (RKS11 or RKS4 or an orthologue thereof). The invention also relates to transgenic plants having introduced therein a nucleic acid or variant thereof encoding at least part of a Leucine Rich Repeat Receptor-Like Kinase (RKS11 or RKS4 or an orthologue thereof), which plants have improved growth characteristics relative to corresponding wild type plants. The present invention also concerns constructs useful in the methods of the invention.

The invention relates to the field of chemical fertilizers, in particular to a compound controlled-release fertilizer special for spring corns and a production method of the compound controlled-release fertilizer. The compound controlled-release fertilizer special for spring corns is composed of the following components in parts by weight: 300-500 parts of nitrogenous fertilizer, 100-170 parts of phosphate fertilizer, 120-235 parts of potassic fertilizer, and 3-15 parts of a slow controlled release long-acting agent. The nutrient supply, the efficiency and the time of the compound controlled-release fertilizer special for spring corns are synchronized with the crop growth requirements, the special proportion of nitrogen, phosphorus and potassium of the special fertilizer in the soil for crops can be enhanced and the formula balanced fertilizing is achieved.

The invention discloses a seedling culture method for strawberry seeds, and belongs to the field of strawberry planting. The method particularly includes the following steps: (1) preparation of seeds; (2) preparation of a seed soaking liquid; (3) seed modification treatment; (4) seed sowing; and (5) management after sowing. The germination rate of the treated seeds is stabilized at more than or equal to 96%, 15 days later after sowing, the seedling emergence rate of more than or equal to 92% can be reached, and cultured seedlings have the advantages of strong disease resistance, excellent nutrient absorption performance, and uniform and vigorous growth vigor, and show unique absorption capacity on iron elements; finally cultured strawberry has high iron content and good edible value.

The present invention concerns a method for improving growth characteristics of plants by increasing activity in a plant of an HKT protein or a homologue thereof. One such method comprises introducing into a plant an HKT nucleic acid molecule or functional variant thereof. The invention also relates to transgenic plants having improved growth characteristics, which plants have increased expression of a nucleic acid encoding an HKT protein. The present invention also concerns constructs useful in the methods of the invention.

A method is provided for raising poultry, such as chickens, for food production. In a first preferred embodiment, the method includes the steps of: providing a facility for housing the poultry, providing at least one light-absorbing ventilation fan associated with the facility for ventilating the facility, exposing an interior of the facility to natural light cycles of an outside environment for a first period and regulating light cycles of the interior for a second period, thereby mimicking daylight duration variation representative of seasonal changes for stimulating sexual development of the poultry. In a second preferred embodiment, the method includes the steps of: providing a facility for housing the poultry, providing at least one light-absorbing ventilation fan associated with the facility for ventilating the facility, limiting exposure of an interior of the facility to light to produce a brown-out effect therein for enhancing physical development of the poultry.

The invention discloses a method for repairing and planting sesbania cannabinain in coastal mud flat of Jiangsu province. The method comprises the following steps: 1, selecting a planting area, and establishing an irrigation facility; 2, performing soil preparation after autumn; 3, pumping water for irrigation before freezing in winter; 4, draining salt water to keep fresh water after ice layers thaw in spring; 5, sowing seeds of sesbania cannabinain; 6, carrying out management in a sesbania cannabinain growing period; 7, pressing salt into soil by using rainfall and water storage in a sesbania cannabinain water-storage field; and 8, carrying out harvesting. The method disclosed by the invention aims at the geographic and climate characteristics of the coastal mud flat of Jiangsu province,and local resources are fully utilized, so that saline-alkali soil in the mud flat can be effectively repaired, and the sesbania cannabinain can be produced in an economic, environment-friendly, high-efficiency and high-yield mode. The method provided by the invention is a saline-alkali soil improvement mode with ecological, economic and social benefits, and a positive promotion effect is achieved on improvement of saline-alkali soil in the mud flat and the sustainable development of agriculture.

The invention discloses a botanical pesticide special for tomatoes. The botanical pesticide is prepared from Chinese brake herb, fructus mume, milkvetch roots, tobacco leaves, folium isatidis, pomegranate seeds, flos rhododendri mollis, herb of sweetclover-like milkvetch, herba portulacae, modified attapulgite and modified diatomite. A traditional Chinese medicine extracting solution is compounded with the modified diatomite and attapulgite to be prepared into the finished pesticide product, all ingredients interact, a good curative effect on common early blight, late blight, powdery mildew, brown patch, blight, a root-knot nematode disease and the like of the tomatoes is achieved, the cure rate is up to 97% or above, relapse is not prone to occurrence, disease resistance cannot be caused, the stability of the pesticide is good, the beneficial ingredients are not prone to decomposition, the quality guarantee period can be prolonged to about three times, after the pesticide is applied, the growth characteristic of tomato plants is better improved, the content of lycopene in all the tomatoes is increased by 40% to 45%, the content of iodine and zinc elements is increased by 10%-20%, and good edibleness is achieved.

The invention discloses feed with a function of improving goat meat quality and belongs to the technical field of feed. The feed is prepared from substances as follows: soybean meal, cottonseed cakes, wheat bran, corn flour, sweet potato flour, vinegar residues, salt, alfalfa, shrimp shell powder, bone meal, olive oil, mountain flour additives and nutrition additives. The prepared feed is good in palatability, rich in nutritional ingredients and reasonable in nutritional ingredient proportion and can effectively improve organism immunity and growth characteristics of goats, thereby improving nutritive value and edible quality of goat meat very well, meat yield can be increased by about 25%, and the economic benefits are higher.

The invention discloses a method for prolonging the flowering phase of North American begonia, and belongs to the technical field of planting. The method includes the steps: (1) seedling selection; (2) seedling modified cultivation; (3) seedling reinforced cultivation. One-age North American begonia seedlings are specially cultivated, growth characteristics of the seedlings are improved by co-treatment of rich water, illumination, temperature and ultrasonic, nutrient absorption characteristics and endogenous hormone secreting characteristics of the seedlings in the flowering phase starting in the middle of April are enhanced, the seedlings are more thoroughly modified under ultrasonic induction and secondary reinforced cultivation, long flowering phase can be continuously kept at a subsequent growth stage, existing annual flowering phase prolonging operation is changed, the flowering phase is fundamentally prolonged, and popularization and use values are high.

The invention discloses pollution-free special pesticide for broccoli. The pollution-free special pesticide is prepared from the following substances: serissa serissoide, pericarpium zanthoxyli, golden cypress, melia azedarach leaves, menthe leaves, grape seeds, radix polygalae, radix sanguisorbae, chrysanthemum leaves, modified attapulgite and modified bentonite. Traditional Chinese medicine extracting liquid, the modified bentonite and the modified attapulgite are compounded to obtain finished-product pesticide; all the components interact, so that the pollution-free special pesticide has a very good healing effect on common downy mildew, soft rot, damping off, sclerotinia stem rot and the like of the broccoli; the healing rate reaches up to 97.5 percent or more and the diseases do not easily recur; drug resistance is not easy to generate and the stability of the pesticide is better; beneficial components are not easy to decompose and the quality guarantee period can be prolonged to be about three times; after the pollution-free special pesticide is applied, growth properties of broccoli plants are improved better; the content of flavonoids in the cultured broccolis is improved by 30 percent to 40 percent and the content of iron and zinc elements is improved by 10 percent to 20 percent, so that the broccoli has very good edible value.

The invention relates to transgenic plants exhibiting enhanced growth rates, seed and fruit yields, and overall biomass yields, as well as methods for generating growth-enhanced transgenic plants. In one embodiment, transgenic plants engineered to over-express glutamine phenylpyruvate transaminase (GPT) are provided.