100 results about "Growth coefficient" patented technology

The invention discloses a factory production method of a dendrobium officinale seedling, which comprises the steps of explant selection, pretreatment, sterilization, lateral bud germination, protocorm inducement, subculture, protocorm differentiation culture, strong seedling rooting culture, and bottle seedling transplant, and a specially-prepared culture medium is used in each step for culturing. The method has the benefits that an explant is easy to take; the sterilization efficiency is high; the growth coefficient is high and stable; components of the culture media are unique, concise and practical; the reproductive cycle is short; the production cost is low; and the produced seedling is stable in hereditary character, strong and tidy, and strong in growth vigor.

The invention discloses a water system connectivity evaluation method. The method comprises the steps of digitalizing a river network of a target area to obtain river network data reflecting water system connectivity; establishing an evaluation system for the water system connectivity according to the river network data; selecting principal components in a system by adopting a principal componentanalysis method, and weighting the principal components by adopting an entropy method; and determining a water system connectivity comprehensive score of the area, thereby analyzing the change of thewater system connectivity, wherein the evaluation system for the water system connectivity consists of a primary index layer and a secondary index layer; quantity connectivity indexes include river network density and a water surface rate; structure connectivity indexes include a river network growth coefficient, an area-length ratio and an average path length; and function connectivity indexes include a clustering coefficient, a node degree and average node betweenness. The indexes are classified and counted; the comprehensive score is obtained through the principal component analysis methodand the entropy method; and the change of the water system connectivity is objectively analyzed, so that a basis is provided for river-lake health and water system function analysis.

The invention provides a tissue culture method of ilex verticillata. The tissue culture method comprises the following steps: (1) selecting an explant from a female plant; (2) processing the explant, namely sterilizing the explant; (3) performing inducing culturing on buds; (4) culturing the buds in enrichment; and (5) performing inducing culturing on roots. The tissue culture method disclosed by the invention is high in survival rate and high in reproduction speed, the growth coefficient achieves 6.1, the rooting percentage achieves 95.2%, and the ilex verticillata female young plant with pure quality is obtained. The method is free from season limitations, and can be used for rapidly propagating the ilex verticillata throughout the year, so that the large production of the ilex verticillata is realized, and an available method is provided for the rapid extension of the ilex verticillata.

The invention discloses a tissue culture rapid propagation method for anisetree barks, which comprises the following steps: (1) taking an anisetree bark seed as an explant, and disinfecting the anisetree bark seed; (2) placing the disinfected explant in an MS basic culture medium to carry out induced germination, so that a sterile test-tube plantlet is obtained; (3) placing the sterile test-tube plantlet in an MS propagation culture medium to carry out rapid propagation culture, so that a cluster bud is obtained; (4) placing the cluster bud in an MS strong seedling culture medium to carry out strong seedling culturing, so that a robust plant is obtained; (5) placing the robust plant in an MS rooting culture medium to culture, so that a complete root-carrying seedling is obtained; and (6) taking the complete root-carrying seedling to carry out hardening, transplanting the obtained seedling to a sand bed to grow for a month, and transplanting the seedling to a field. The growth coefficient of anisetree bark cluster buds obtained by using the culture method disclosed by the invention reaches 8-12 times, the rooting rate of the obtained tissue culture seedling is over 85%, and the survival rate of seedlings transplanted to a seedbed is over 90%, thereby effectively solving the large-scale seedling raising problem of anisetree barks.

The invention provides a tissue culture and rapid propagation method for dracaena cochinchinensis. The tissue culture and rapid propagation method comprises the following steps: (1) taking a dracaena cochinchinensis seed as an explant and disinfecting; (2) putting the disinfected explant into an MS (Murashige and Skoog) culture medium to be induced and burgeoned to obtain a sterile test-tube plantlet; (3) putting the sterile test-tube plantlet into the MS culture medium to be subjected to test-tube plantlet rapid propagation and culture to obtain cluster buds; (4) putting the cluster buds into an MS strong seedling culture medium to carry out strong seedling culture to obtain a strong plant; (5) putting the strong plant into an MS rooting culture medium to be cultured to obtain a complete seedling with roots; and (6) taking the complete seedling with the roots and carrying out domestication; and transplanting the seedling into a sand bed to grow for one month and then transplanting the seedling to a large field. With the adoption of the culture method, the growth coefficient of the cluster buds of the obtained dracaena cochinchinensis reaches 10-15 times; the rooting rate of an obtained tissue culture seedling is more than 95% and the survival rate of a transplanted seedling bed is more than 90%, so that the large-scale seedling growth problem of the dracaena cochinchinensis is solved effectively.

The invention discloses a tissue culture rapid propagation method of anoectochilus formosanus, and belongs to the technical field of plant tissue culture. By adopting the tissue culture rapid propagation method, the survival rate of transplanting and the growth coefficient can be increased, and the good characters and the good drug properties of the Emei anoectochilus formosanus can be kept. The tissue culture rapid propagation method comprises the following steps: selecting wild Emei anoectochilus formosanus growing in May to October, preparing an explant, carrying out disinfection treatment, inducing cluster buds, carrying out secondary culture and rooting and strengthening culture, and transplanting to greenhouses for culturing, and rapidly propagating the anoectochilus formosanus through hormone induction. The tissue culture rapid propagation method can be used for ensuring the good characters and the good drug properties of the Emei anoectochilus formosanus, is simple and low in cost and can realize industrialized seedling production. The percentage of inducing the cluster buds and rooting by adopting the method reaches above 90%, the survival rate of transplanting is above 95%, and the growth coefficients within four months can reach 8-10 times. The Emei anoectochilus formosanus produced by adopting the method does not mutate, is high in yield, low in cost and short in period and has relatively strong market competitiveness.

The invention discloses a zanthoxylum nitidum tissue culture medium. The medium comprises a subculture medium and a rooting medium. Improvement is carried out on the basis of an H medium, the macroelement usage is adjusted, and the iron vitriol content is increased. The medium is suitable for culturing zanthoxylum nitidum and single zanthoxylum nitidum tissues from different producing areas. The subculture period takes 20-25 days, the growth coefficient is 4-6, subculture seedlings grow well, small seedlings are thick and order, the leaf color is green, no etiolated seedlings are produced, and the seedling yield is high. The rooting rate of the rooted seedlings can reach 90%, the rooting is quick; and when the temperature is at 25 plus or minus 2 DEG C, the average rooting time is 10-15 days, a root system well grows, the rooting number is larger and averagely 3-5 roots, the seedling is good in lignification, the transplanting survival rate reaches above 90%, and the outplanting time of bottled seedlings is about 30 days averagely.

The invention relates to a method for cultivating haworthia maughanii tissue. The method comprises the following steps of: preparing culture medium, selecting and sterilizing explants, callus induction, adventitious shoot induction, adventitious bud increment, strong seedling cultivation, adventitious bud root induction, somaclone domestication, and transplantation. The basic culture medium selects an MS culture medium; the dosage of saccharose is 20-40g / L; a coagulator is agar powder; the usage is 8-9g / L; the pH of the culture medium is adjusted to 5.7-5.8 before being charged; callus induction culture medium is 3.0-5.0mg / L of MS+6-BA and 0.05-0.2mg / L of NAA; adventitious shoot induction culture medium is 1.0-3.0mg / L of MS+6-BA and 0.05-0.2mg / L of NAA; adventitious bud increment culture medium is 0.5-1.0mg / L of MS+6-BA and 0.05-0.2mg / L of NAA; strong seedling culture medium is 0.1-0.2mg / L of MS+6-BA and 0.01-0.1mg / L of NAA; and root cultivation medium is 0-1.0mg / L of MS+IBA or 1 / 2MS + 0-1.0mg / L of IBA. The method has the advantages that the growth coefficient of the built rapid propagation technique system for haworthia maughanii tissue tissue cultivation is 2-2.5; the rooting rate is 100%; and the transplanting survival rate is over 98%.

The invention discloses a rapid propagation method for holcoglossum flavescens. Rapid propagation of holcoglossum flavescens is completed through the steps of seed germination, induction of protocorm, differentiation culture, strong seedling culture, rooting culture, acclimatization and transplant and the like. Compared with a traditional division method, the growth coefficient of holcoglossum flavescens is improved by more than 5 times, the planting percent reaches 95%, the survival rate of transplanting reaches more than 85%, meanwhile, the method has the advantages of being large in increment coefficient, strong in tillering capacity, high in growth speed, strong in plant, low in browning ratio, high in survival rate of transplanting, and low in cost, culture medium is easy to prepare, and the method can be applied to large scale industrial production.

The invention relates to a tissue culture method for ornithogalum caudatum. The method comprises the following steps of: preparing culture media, selecting and sterilizing explants, primarily cultivating, inducing adventitious buds, growing of the adventitious buds, performing root induction on the adventitious buds, and domesticating and transplanting tissue culture seedlings. The basic culture medium selects an MS culture medium; the dosage of saccharose is 20-40 g / L; a coagulator is agar powder, and the dosage of the coagulator is 8-9 g / L; the pH of the culture medium is adjusted to 5.7-5.8 before being sub-charged; the initial culture medium comprises 0.1-0.5 mg / L of MS+6-BA and 0.05-0.2 mg / L of NAA; the adventitious bud induction culture medium comprises 2.0-4.0 mg / L of MS+6-BA and 0.05-0.2 mg / L of NAA; and the adventitious bud increment culture medium comprises 0.5-3.0 mg / L of MS+6-BA and 0.05-0.2 mg / L of NAA; and the rooting medium comprises 0-0.5mg / L of MS+IBA, or 0-0.5mg / L of 1 / 2MS+IBA. The method has the advantages that a built tissue culture and rapid propagation technique system for ornithogalum caudatum can reach a growth coefficient of 3-5, a rooting rate of 100% and a transplanting survival rate of 100%, and the tissue culture seedlings are healthy and even. Therefore, the method is suitable for detoxification and large-scale production of excellent ornithogalum caudatum seedlings.

The invention relates to a method for determining the reliability growth trend of a railway vehicle and predicting a fault rate, and the method comprises the steps: quantitatively giving a reliability growth coefficient according to the stage fault and operation information of the railway vehicle in operation, and judging that the reliability growth trend of the railway vehicle is increase, constant or decrease according to the reliability growth coefficient. The method can quantitatively calculate the subsequent one time point of the railway vehicle, can quantitatively determine whether the subsequent performance of the railway vehicle can meet the requirements of a technical standard or not according to the reliability performance which can be achieved by a current reliability growth mechanism, determines a subsequent working method according to the conformity, determines whether there is a need to change a conventional reliability growth management mechanism or not, and guarantees the achieving of a reliability target of the railway vehicle. However, a conventional method cannot judge whether the subsequent performance of the railway vehicle can meet the requirements of the technical standard so as to determine whether to improve the reliability growth mechanism.

The invention discloses a method for controlling and reducing the output of sludge in a biological wastewater treatment system through adding a bacterial agent. The bacterial agent is characterized by high removal efficiency of organic matters and low self growth coefficient. The bacterial agent is directly or indirectly added in a biological wastewater treatment reactor; the addition amount is 5 to 20 percent; and the reduction amount of the sludge can achieve 10 to 30 percent. Compared with the traditional physicochemical method, the sludge reduction method has the advantages of simple operation, low cost, safety and environmental protection, quick curative effect, less influence on the prior system function, etc.

The invention belongs to the technical field of plant tissue culture and discloses a dendrobenthemia japonica tissue culture method. The dendrobenthemia japonica tissue culture method comprises the steps of pretreating, performing primary culture, enrichment culture and strong seedling culture, planting in a rooting medium and transplanting, wherein in the culturing process, the external conditions are as follows: pH is 5.8-6.0, the culture temperature is 23-25 DEG C, the illumination period is 8-10h / d, and the illumination intensity is 1800-2000lux. The culture method has the benefits that 1. with the adoption of the culture method, a growth coefficient is high, a rooting rate is high, and a cultured plant has excellent growth characteristic; and 2. large scale production can be achieved by the culture method, and the reproduction efficiency is high.

The invention discloses a tissue culture method for in-vitro regeneration of a grape stem segment explant. The method comprises the following steps: selecting an appropriate grape stem segment explant, sterilizing the grape stem segment explant, inoculating the sterilized grape stem segment explants, building an in-vitro regeneration system for the grape stem segment explant, carrying out grape test-tube plantlet multiplication culture, and performing grape test-tube plantlet rooting culture, thereby achieving regeneration culture of grape tissues. Compared with existing similar studies, the method has the advantages that the adventitious bud has the high regeneration rate, the browning rate is low, the growth coefficient can reach 5.6, and the rooting rate can reach 98.7%. The method is applied to the rapid in-vitro grape reproduction, the reproductive cost is reduced greatly, and the method can be used for provided convenience for correlational research of grape breeding such as grape biotechnological breeding and molecular breeding.

The invention provides a tissue culture and rapid propagation method for dendrobium, belonging to the technical field of tissue culture of plants. The method comprises the following steps: explant disinfection, induced culture, subculture, strong seedling culture and the like, wherein a combined disinfection manner of alcohol disinfection and plant disinfectant disinfection is adopted in the explant disinfection step, and a plant disinfectant contains the effective components of glycyrrhizic acid, lemon juice and a honeysuckle leaf extract; and horseradish tree leaf juice and okra juice are added as nutrients into culture media used in each culture stage. The method has the beneficial effects that the damage caused to an explant is small; and by utilizing a culture medium formula, the induced differentiation rate of dendrobium can be remarkably increased, the growth coefficient and rooting rate of the explant can be increased, the culture period can be shortened, and the quality of a tissue culture seedling of dendrobium can be improved.

The invention discloses a detoxifying tissue-culture rapid propagation method for fruit cane. The method disclosed by the invention comprises the following steps: 1) selecting an explant; 2) detoxifying under high temperature; 3) detoxifying and inoculating stem tips and acquiring an aseptic seedling; 4) detecting pathogene; 5) performing subculture multiplication; 6) rooting and hardening seedlings; and 7) heeling in, transplanting and managing. According to the invention, the warm water treatment is effectively combined with the manual climatic box high-temperature pregermination, the cane buds are subjected to detoxifying treatment and the detoxifying rate is high; 3-5mm stem tips are selected for starting the inoculated culture and the survival rate is 90% or above; the culture process is started, the browning is effectively avoided and the yield of the sterile strain is increased in the manner of changing the manner of starting the culture medium and adding PVP into the culture medium; MET component is added into a rooting culture medium, so that quick rooting and more and regular roots rooting in the rooting process can be realized; low-concentration hormone is added into the culture medium in the tissue-culture rapid propagation process, the influence of the hormone on the tissue culture seedling is extremely reduced, the original nature of the variety is kept, the growth coefficient is 2-4 times and the purposes of purification, rejuvenation and rapid propagation can be achieved.

The invention discloses a tissue culturing propagation technology for peach resistance stock No.1 among peach stock. The tissue culturing propagation technology includes the working procedures of explant collecting and pretreatment, aseptic seedling establishing, enrichment culturing, extending culturing, rooting culturing, greenhouse acclimatization and transplant and the like. Culturing is carried out through two kinds of enrichment media alternately, a balance point is found between enrichment efficiency and vitrification, and technical reference is also provided for tissue culturing rapid propagation of other peach materials. The technology is high in pertinence; the primary induction germination rate of explant is higher than 80%, the growth coefficient of enrichment culturing can be 2 to 5, the rooting rate obtained after extending culturing is carried out to strengthen seedlings can be higher than 80%, and the acclimatization and transplant survival rate is about 90%. By means of the tissue culturing propagation technology, clone nursery stock of the peach resistance stock No.1 can be continuously produced under the tissue culturing condition, and the technology is used for achieving the aim of replanting peaches of an aged peach area.

The invention discloses an idesia leaf tissue culture method, which comprises the following steps of (1) explant selection and treatment; (2) callus induction and culture; (3) differentiation and proliferation culture; (4) strong seedling culture; (5) rooting culture; (6) transplant. Compared with the prior art, the idesia leaf tissue culture method provided by the invention has the advantages that a large number of high-quality seedlings are produced within a short period, less explants are used, the reproduction speed is fast, the growth coefficient and the rooting percentage are high, and the reproduction rate is high.

The invention discloses a detoxification and rapid propagation method for edible lily. The detoxification and rapid propagation method comprises the following steps: (1) selecting and treating explant; (2) sterilizing the explant, and picking off growing points; (3) starting culture; (4) performing RT-PCR virus detection and cluster bud induction culture; (5) performing bulb induction and rooting culture. By adopting the detoxification and rapid propagation method, main viruses in the lily can be removed for 100%, particularly, multiple times of tests show that the viruses in infected lily explant can be successfully removed, and moreover the survival rate is greater than 85%; in addition, the growth period of the lily can be shortened while a relatively high growth coefficient is obtained, a great amount of detoxified lily seedlings can be cultured within a short time, and thus the production requirements can be met.

The invention discloses a method for rapid propagation of virus-free artemisia seleirgensis seedlings. Middle and upper section stems of healthy artemisia seleirgensis plants having bright variety characteristics are selected to serve as explants, and finally complete virus-free artemisia seleirgensis seedlings are formed through axillary bud induction, detoxification culture, rooting culture and transplanting and field planting. The virus-free artemisia seleirgensis seedlings have a quick propagation speed, good virus-free effects, good growth vigor and good genetic stability, the whole process from explant inoculation to formation of transplantable tube seedlings only needs about 90 d, the growth coefficient can reach 20, the problems of severe seedling viruses and degradation of yield and quality caused medium and long term asexual reproduction in artemisia seleirgensis growth are solved, the virus-free artemisia seleirgensis seedlings propagate quickly, and large-scale production of the virus-free seedlings is met.

The invention belongs to the technical field of soil remediation, and particularly relates to application of a g-C3N4 / Fe3O4 composite material to polycyclic aromatic hydrocarbon contaminated soil remediation. Through compounding of Fe3O4 nano particles and g-C3N4, the visible light absorption performance of the material is improved, compounding of photo-generated electrons and holes is effectivelyinhibited, and accordingly, the photocatalytic performance of the material is improved. A method for applying the g-C3N4 to polycyclic aromatic hydrocarbon contaminated soil remediation comprises thesteps of contaminated soil detection, contaminated soil breaking screening, and mixing of the g-C3N4 / Fe3O4 composite material and the contaminated soil. After the mixed soil is spread, polycyclic aromatic hydrocarbon in the soil can be removed through light, and the pH value of the soil does not need to be adjusted in the whole soil remediation process. According to the remediation method, the phytotoxicity of pollutants in the soil can be greatly reduced, and the difference between the growth coefficient of a plant growing on the remediated soil and the growth coefficient of a plant growingon the unpolluted soil is unobvious.

The invention relates to a rapid propagation method of lindera communis. The rapid propagation method comprises the steps of obtaining of aseptic seedlings, induction of cluster buds, multiplication of cluster buds, rooting induction, and acclimatization and transplanting. The rapid propagation method of lindera communis is simple and convenient to operate, and the lindera communis is high in growth coefficient, high in survival rate and stable in inheritable characters; as a result, the rapid propagation method is capable of obtaining a large quantity of lindera communis seedlings in short time and lays a foundation for large-scale development and effective utilization of lindera communis.

The invention belongs to the field of plant tissue culture, and provides an in-vitro rapid propagation method for lonicera praeflorens. The method comprises the steps that tender stems of lonicera praeflorens are used as an explant, an MS is used as a minimal medium, plant growth regulators 6-BA and NAA are added for inducing axillary buds to germinate and grow; then, subculture multiplication, rooting induction and acclimatization and transplanting are performed, and a large number of regeneration plants can be obtained within a short period of time. The effective way is provided for rapid propagation of high-quality seedlings of lonicera praeflorens, the growth coefficient is high, the induction rooting rate is high, and transplanting and survival are easy. The requirement for landscaping in early spring in the Northeast China region can be met to a great extent, and a foundation is laid for further comprehensive development and utilization.

The invention discloses a litsea cubeba extracting solution and a preparation method and application thereof. The litsea cubeba extracting solution is prepared from litsea cubeba, water and an additive, wherein the additive includes vitamin C and / or polyvinylpyrrolidone, and the mass ratio of the litsea cubeba to the additive is (4-8):(0.5-2.5). The preparation method comprises the steps that thelitsea cubeba, by mass part, is ground into powder to obtain litsea cubeba powder; the litsea cubeba powder is mixed with, by mass part, the additive and part of water to obtain a mixed solution; after the mixed solution is subjected to heat preservation and centrifugalization, supernatant liquid is obtained and placed into a container, the volume is set by using water to a scale, and then the litsea cubeba extracting solution is obtained. The litsea cubeba extracting solution is used in litsea cubeba tissue culture, the inductivity and the growth coefficient of litsea cubeba cluster buds canbe obviously improved, therefore, the in-vitro rapid propagation efficiency of the litsea cubeba is improved, and the development of the litsea cubeba industry is promoted.

The invention relates to a tissue culture and propagation method of cinnamomum camphora. According to the method, the combination of improved DCR culture medium and MS culture is used for the first time; through the measures of improving the DCR culture medium element content, regulating the plant hormone level, increasing active carbon, regulating and controlling the temperature illumination, increasing the seeding strengthening culture and the like, the invention provides a cinnamomum camphora tissue culture system with the advantages that the bud induction rate reaches up to 98 percent or higher; the growth coefficient with an average of 4.8; the browning rate is smaller than 3.2 percent; the seeding strengthening rate (3cm or higher) is 81 percent or higher; the transplanting survival rate reaches 87 percent or higher; the heavy demand of markets on the cinnamomum camphora is met.

The invention researches a rapid propagation method of elaeagnus pungens. The rapid propagation method comprises the steps of obtaining a sterile explant, inducing an axillary bud, proliferation of multiple shoots, rooting culture, acclimatization and transplantation, etc. The elaeagnus pungens prepared by the method disclosed by the invention is good in propagation rate, high in growth coefficient and short in growth cycle, and a great amount of elaeagnus pungens seedlings can be obtained within a short period.

The invention relates to the technical field of tissue culture, in particular to a medium and a method for tissue culture of euonymus maackii. The tissue culture medium provided by the invention is applicable to a variety, namely 'Jinzhi Yuye' (euonymus macckii rupr.), of the euonymus maackii; on the basis of a 1 / 2MS medium, KT, NAA, TDZ and gibberellin are added for promoting callus induction; on the basis of an MS medium, KT and TDZ are added for promoting axillary bud induction; and a WPM medium is taken as a seedling hardening medium. Induced by the medium provided by the invention, a growth coefficient can be increased to 9.72, and a survival rate of seedlings can reach 93% or above.

The invention discloses a hemiptelea tissue culture seedling subculture method. The method includes the following sequential steps that 1, a hemiptelea explant is treated, wherein an explant branch is pruned, cleaned and put in clean water for germination accelerating; 2, an aseptic seedling is obtained, wherein axillary buds germinating on the explant branch are disinfected, an initial medium is inoculated with the axillary buds, and the aseptic seedling is obtained; 3, the aseptic seedling is subcultured, wherein the aseptic seedling is transferred into a subculture medium for subculture multiple times. The hemiptelea tissue culture rapid propagation method is provided for the first time, nutritional ingredients in the mediums are adjusted by adding coconut milk, and the yellowing phenomenon generated after multiple times of subculture of hemiptelea tissue culture seedlings is solved; the growth coefficient of subculture is remarkably increased; the subculture frequency of subculture is increased, and if the method that five-generation propagation and one-generation rooting are alternately carried out is adopted, hemiptelea subculture can be infinitely carried out theoretically. The production cost is reduced, and large-scale and industrialized production of hemiptelea is possible.

The invention discloses a tissue culture and rapid propagation method for sunlight cherries. The method comprises the following steps: setting a basic culture medium and optimizing the proportion of different hormone components and concentration levels; by taking an annual semi-lignified branch of an excellent adult strain as an explant, performing bud induction, bud proliferation and rooting culture to form an integrated plant; and transplanting the integrated plant to a matrix to obtain robust nursery stocks which are order in growth and consistent in phenotype. The method disclosed by the invention has the advantages of being high in propagation coefficient, short in culture period, free of limitation on seasons and etc.; the inductivity reaches 100%, the growth coefficient reaches 5.1, the proliferated bud is fast to elongate and grow, the bud height after being cultured for 18d reaches 2.5cm, and the effective bud reaches 35 / bottle averagely; by rooting culture for 10d, the rooting rate is greater than 98%, and the number of roots reaches 5 / strain above; the tissue culture rooted seedlings are robust, and the transplanting survival rate reaches up to 92%; in actual production application, annual scaled fast seedling can be performed to produce sunlight cherry seedlings which are robust, order and consistent, and the method is wide in application prospect.

A method for culturing and breeding dendrobium guangxiense tissue comprises the following steps that 1, a newly-germinating tender shoot of a healthy dendrobium guangxiense plant is taken as an explant to be sterilized; 2, the sterilized explant is placed in an MS minimal medium to be induced, and a sterile test-tube plantlet is obtained; 3, the sterile test-tube plantlet is placed in the MS minimal medium to be fast bred and cultured, and multiple shoots are obtained. The growth coefficient of the dendrobium guangxiense multiple shoots obtained through the culturing method reaches 25-30 times, a high-quality dendrobium guangxiense seedling with a high survival rate can be provided within a short time, and the large-scale seedling growing aim of dendrobium guangxiense is effectively achieved.