32 results about "Dracaena cochinchinensis" patented technology

The invention provides a tissue culture and rapid propagation method for dracaena cochinchinensis. The tissue culture and rapid propagation method comprises the following steps: (1) taking a dracaena cochinchinensis seed as an explant and disinfecting; (2) putting the disinfected explant into an MS (Murashige and Skoog) culture medium to be induced and burgeoned to obtain a sterile test-tube plantlet; (3) putting the sterile test-tube plantlet into the MS culture medium to be subjected to test-tube plantlet rapid propagation and culture to obtain cluster buds; (4) putting the cluster buds into an MS strong seedling culture medium to carry out strong seedling culture to obtain a strong plant; (5) putting the strong plant into an MS rooting culture medium to be cultured to obtain a complete seedling with roots; and (6) taking the complete seedling with the roots and carrying out domestication; and transplanting the seedling into a sand bed to grow for one month and then transplanting the seedling to a large field. With the adoption of the culture method, the growth coefficient of the cluster buds of the obtained dracaena cochinchinensis reaches 10-15 times; the rooting rate of an obtained tissue culture seedling is more than 95% and the survival rate of a transplanted seedling bed is more than 90%, so that the large-scale seedling growth problem of the dracaena cochinchinensis is solved effectively.

The invention discloses a method of propagation by cuttings for dracaena cochinchinensis SC chen, which consists of selecting branches of dracaena cochinchinensis SC chen trees with an age of over two years, cutting the upper end of the branches, cutting the lower end to 40-50 degree of inclined surface, sealing the upper extreme tangent plane of the grafting ear with paraffin wax, immersing the lower end tangent plane in aqueous solution of root-producing powder, carrying out cuttage by the conventional process and transferring into large field planting.

The present invention discloses artificial propagation process of Dracaena cochinchinensis. The propagation process includes seedbed preparation, seed treatment, seeding, seedbed management, transplanting, field management and other steps. The present invention makes it possible for planting Dracaena cochinchinensis in large scale and protecting the wild rare medicine source plant resource.

The invention relates to a cultivation method for dracaena cochinchinenisis, belonging to the plant culture technical field. The planting field applied in the method is dry tropical or subtropical mountainous forest which is low in altitude and is sun-exposed. The specification of the planting pond is forty multiplies forty multiplies forty centimeter, the plant row spacing is one point five multiplies one point five meter and two hundred and ninety six plants are planted every unit of area; one to three kilograms of organic fertilizer is added and mixed evenly with the surrounding earth when the pond is backfilled to the original plane. The pond is built in a pyramid shape by piling soil based on the backfilled original plane and is twenty to twenty five centimeters over the original plane. Then the bags of the dracaena seedling are removed, with two thirds of the seedling planted on the pyramid point and pressed with soil and one third of the seedling exposed to the soil surface. The root is required to naturally stretch without winding. The exposed part does not earth up for free growing. After planting, cultivation is needed for at least two times every year. The first cultivation is around April to May and each plant is fertilized with ten to twelve granules of compound fertilizer in the market; the second cultivation is at the end of the autumn and at the beginning of the spring and each plant is fertilized with one to one point five kilograms of organic fertilizer. The invention has the advantages that the method is convenient and high in seedling survival rate.

The invention discloses a dragon's blood tree leaf extract product, and a preparation method, a pharmaceutical composition, a preparation, and applications thereof. According to the preparation method, the dragon's blood tree leaf extract product is prepared by taking dried leaves of Dracaena cambodiana Pierreex Gagnep. or Dracaena cochinchinensis (Lour.) S. C. Chen as raw materials; total flavones content of the dragon's blood tree leaf extract product is 1.0% or higher; and no tannin, resin, or oxalate is detected. The dragon's blood tree leaf extract product possesses excellent effects on preventing and controlling osteoporosis and reducing blood glucose, and clinical application prospect is promising. According to the preparation method, the raw materials are easily available; operation is simple; the preparation method is economical and friendly to the environment; product quality is stable and controllable; and the preparation method is suitable for industrial scale production.

Provided is a dracaena cochinchinensis loose callus induction method. The method comprises the following steps: firstly, dracaena cochinchinensis seeds or buds are taken as explants, and disinfection is carried out; secondly, the explants after disinfection are placed in an MS basal medium, and buds or clustered buds are generated through induction; thirdly, the aseptic buds are placed in MS, B5, N6 media with 6-benzyladenine 6-BA and naphthylacetic acid NAA, and cultured, and callus is obtained; fourthly, the callus is placed in induction media with different hormones and cultured, and loose callus is obtained. The callus obtained through the method is peak green or milk white and loose and has a strong propagation capability. A lot of well-grown loose callus can be induced in short time, and materials are provided for production of dracaena cochinchinensis total alkaloids through cell suspension culture.

A method for promoting the germination of Dracaena phoenix-leaves seeds, comprising the steps of: (1) getting mature and plump Dracaena phophyllum seeds, removing the seed coat, and successively using carbendazim, naphthaleneacetic acid (NAA), gibberellin (GA3) Soaking treatment at different times and different concentrations; (2) Place the treated seeds in the sterile fine sand in the petri dish, and put 30 seeds in each dish; (3) Set the temperature of the light incubator at 15 °C, 20 °C, 25 °C, and set different light time, the standard for seed germination is that the radicle breaks through the seed coat by 1mm and is considered as germination. A method for promoting seed germination of Dracaena phoenix leaf obtained by adopting the method of the invention has simple operation method and high seed germination rate. This method breaks the dormancy of Dracaena seeds and promotes their germination, provides technical support for accelerating the breeding of D. In order to provide more high-quality materials for the medical extraction of "dragon's blood" and further meet market demand.

The invention relates to a separating preparation process of high-purity broussin; and the method comprises the specific steps of: using dracaena cochinchinensis resin as raw materials; crushing the raw materials; adding 4-10 times of ethanol of 65-90% to the raw materials for ultrasonically extracting the raw materials; adding a proper amount of diatomite to an extracting liquor so as to preserve the heat of the extracting liquor and decolorize the extracting liquor; concentrating a decolorized solution; enriching the decolorized solution by using a polyamide resin column; eluting the decolorized solution by using 75-85% of ethanol solution; concentrating an eluting solution; drying the eluting solution; and separating a dry product by using a high speed counter-current chromatography so as to obtain the broussin. The separating preparation process of the high-purity broussin, provided by the invention, has the advantages of simplicity and convenience in operation, high separating efficiency and good product purity and is suitable for industrial production.

The invention relates to a dracaena cochinchinensis quality detection method. The detection method comprises a step of measuring the content of 7-hydroxyl-4'-methoxylflavane and / or pterostilbene in dracaena cochinchinensis extract (dracaena cochinchinensis general flavone) through a wavelength conversion HPLC method, wherein the content of 7-hydroxyl-4'-methoxylflavane in the dracaena cochinchinensis general flavone is not less than 0.10 wt%, and / or the content of pterostilbene in the dracaena cochinchinensis general flavone is not less than 0.20 wt%. Through the optimization of the chromatogram conditions and application of the wavelength conversion HPLC method, two low polarity effective components in dracaena cochinchinensis can be simultaneously measured, so the method has a very important meaning in the overall quality control of medicinal materials and extracts.

The invention provides a rare and endangered medicinal plant dracaena cochinchinensis water planting rapid rooting and seedling method. The method comprises the following steps that dracaena cochinchinensis branches with the length being 20 m and the average diameter being 2 cm are cut out to serve as cuttings, 2 cm of the morphological lower ends of the cuttings are soaked in a solution with theABT rooting powder content being 500 mg / kg for 30 min and then taken out, the cuttings soaked in the rooting powder solution is placed in a clean bucket, clear water is injected in the bottom for 5 cmin depth, water changing is conducted once every 5-10 days, it is guaranteed that the water is clean, the environment temperature is kept at 20-25 DEG C, and after 60 days, the sprouted and rooted cuttings are transplanted and planted. Compared with seedlings cultivated through a tissue culture method, dracaena cochinchinensis seedlings cultivated through the method has the advantages of being low in cost, high in production value and the like, and the material basis, scientific basis and technical support are provided for species return to nature and population reconstruction realization.

The invention discloses an application of two quadruple lactone antibiotics as anti-MRSA drugs and an extraction and separation method of the two quadruple lactone antibiotics. According to the methoddisclosed by the invention, two quadruple lactone compounds, namely Dinactin and Trinactin, are separated from Dracaena cochinchinensis endophyte Streptomyces punctatus S009 for the first time; in-vitro anti-MRSA activity tests are carried out on the two separated compounds, and results show that the Dinactin and the Trinactin have a stronger inhibition effect on a plurality of clinically separated MRSA strains than a positive reference substance vancomycin, so that the Dinactin and the Trinactin have a better inhibition effect on multiple kinds of clinically separated MRSA strains than a positive control substance, vancomycin, and a molecular docking result shows that such kind of compounds has strong binding activity with anti-MRSA target protein PBP2a. Therefore, the invention not onlycan provide lead compounds for developing a novel anti-MRSA drug, but also can effectively protect rare and endangered medicinal plant resources, and is of great significance for reducing the damageto the diversity of wild medicinal plants.

The invention relates to a drug for treatment of osteoporosis, wherein the drug consists of oysters, premna fulva craib, dracaena cochinchinensis, soft-shelled turtle, herba lespedezae cuneatae, davallia formosana, tuber dioscoreae persimilis, membranaceous beautyleaf root, fennel and a pearl powder. The drug has the effect for relief and treatment of osteoporosis, and has the advantages of low price, and no toxic or side effects, and is safe and reliable.

The invention relates to a medicine for treating sequela of apoplexy, containing the following traditional Chinese medicine components in weight ratio: pseudo-ginseng, Yulangsan, turmeric, erycibe obtusifolia, astragalus membranaceus, illigera trifoliata, polygala tenuifolia, polygonatum sibiricum, sialic acid, persian walnut seed, agilawood, ganoderma lucidum, salvia miltiorrhiza, gastrodia elata, dracaena cochinchinensis, folium ilicis hainanensis and golden-side leeches. The pharmaceutical composition can relieve the pain of a patient, reduce fatality rate and disability rate, and improve the quality of life, has no toxic or side effect, treats both symptoms and root causes, and has an important clinical value for preventing and treating stroke.

The invention relates to a medicine for treating sweat stain. The medicine is prepared by using Mussaenda Pubescens Ait.f., honeysuckle flower, Dracaena cochinchinensis, Evodia lepta (Spreng.) Merr. wood, Spreading Hedyotis Herb, folium isatidis and Corydalis saxicola as raw materials. The medicine has an obvious treatment effect on sweat stain, is low-cost, has no toxic or side effect and is safeand reliable.

The invention belongs to the field of extraction of traditional Chinese medicines, and in particular relates to a method for simultaneous preparation of 7-hydroxy-4 '-methoxy flavan and pterostilbene. The method includes the steps of: 1) taking dracaena cochinchinensis grease-containing wood or ethanol extract thereof, grinding, adding petroleum ether to extract, filtering to obtain filter residue for standby use; 2) using chloroform for reflux extraction of the obtained filter residue, and recovering the chloroform to obtain chloroform extract; 3) using methanol to dissolve the obtained chloroform extract, performing column chromatography, eluting with methanol with every 250ml being one flow part, and merging 2-8 flow parts; 4) concentrating the merged 2-8 flow parts to dry, dissolving with methanol, using a reverse phase silica gel column for column chromatography, eluting with methanol with every 100ml being one flow part, and respectively merging 14-18 flow parts and 20-27 flow parts; and 5) concentrating the respectively merged 14-18 flow parts and 20-27 flow parts to dry, and respectively recrystallizing to obtain the two compounds of 7-hydroxy-4'-methoxy flavan and pterostilbene. The two compounds of 7-hydroxy-4 '-methoxy flavan and pterostilbene are firstly and simultaneously prepared from dracaena cochinchinensis by the method.

The invention provides a compound preparation which comprises dracaena cochinchinensis resin or daemonorops draco resin and other components and has the effects of trauma treatment, tissue repair, angiitis treatment and the like. The compound preparation is characterized in that the ratio of the dracaena cochinchinensis resin or the daemonorops draco resin to borneol is 9:1, and the ratio of the dracaena cochinchinensis resin or the daemonorops draco resin to berberine to borneol is 9:4:1, or the ratio of the dracaena cochinchinensis resin or the daemonorops draco resin to the berberine to the borneol is 1:1:0.15. The invention also provides a preparation method of the compound preparation and application of the dracaena cochinchinensis resin or daemonorops draco resin compound in the preparation of compound gels, liniment, cataplasm, adhesive bandages (such as patches) and other preparations. Compared with two positive medicines (Madecassol, jingwanhong soft plaster), the dracaena cochinchinensis resin or daemonorops draco resin compound preparation has the obvious effects of promoting wound healing and tissue repair. The acute toxicity test and the long-term toxicity test show that the preparation is safe to use.

The invention discloses a callus induction and anti-browning method for dracaena cochinchinensis. The method comprises the following steps: (1) using dracaena cochinchinensis seeds as explants for sterilizing; (2) putting the sterilized explants into an MS minimal medium for performing induced budding; (3) putting germ-free shoots into the MS minimal medium for culturing to obtain callus; (4) putting the callus into an MS enrichment medium for culturing to obtain a large amount of callus; and (5) putting the large amount of callus into an MS anti-browning culture medium for culturing to obtain the callus with fluffy texture. The callus obtained by using the method is viridis or cream yellow, high in growing speed and difficult to brown, and can produce a large amount of callus with good growth trend and low browning rate by means of induced proliferation; and therefore, high-quality materials are provided for extracting dragon's blood in medical science, and the market requirement can be further met.

The invention relates to a Chinese patent medicament for treating bone traumatism, which comprises the following components of raw materials in part by weight: 30 to 70 parts of sanchi, 5 to 20 parts of dracaena cochinchinensis, 10 to 50 parts of myrrh, 20 to 50 parts of pyrite, 20 to 50 parts of fortune's drynaria rhizome, 10 to 50 parts of dahurian angelica root and 2 to 15 parts of borneol. The preparation method comprises the following steps: putting the sanchi, the fortune's drynaria rhizome and the dahurian angelica root together, extracting the raw materials with ethanol to prepare clear ointment, decocting the produced dregs of a decoction with water, and mixing the clear ointment with the decoction to prepare fine powder; mixing the dracaena cochinchinensis, the myrrh and the pyrite to prepare fine powder, mixing the two fine powders, and adding starch and ethanol into the fine powders to prepare soft materials; and granulating the soft materials in a granulator, and filling the granules into capsules. The Chinese patent medicament has the characteristics of quick cure of bone traumatism, stable curative effect, no toxic or side effect and low cost. The preparation process has the characteristics of simple preparation and convenient popularization.

The invention discloses an extraction method and preparation of dracaena cochinchinensis extract prepared by artificial induction. A preparing method of the dracaena cochinchinensis extract prepared by the artificial induction includes: crushing the dracaena cochinchinensis which generates induction products through artificial induction (biological induction and chemical induction), adding 95% ethanol with the weight being 6-15 times of that of the crushed dracaena cochinchinensis, performing reflux extraction twice, filtering extract, sieving with a 200-mesh sieve, and concentrating the filtrate into extract or drying the filtrate into dry extract, wherein each extraction lasts 1-4 hours. Researches show that the extract has certain analgesic and anti-inflammation effects, so that analgesic and anti-inflammation tablets, granules, capsules or other conventional preparations can be prepared by adding pharmaceutically acceptable auxiliaries into the crushed extract.

The invention belongs to the field of extraction of traditional Chinese medicines, and in particular relates to a method for simultaneous preparation of 7-hydroxy-4 '-methoxy flavan and pterostilbene. The method includes the steps of: 1) taking dracaena cochinchinensis grease-containing wood or ethanol extract thereof, grinding, adding petroleum ether to extract, filtering to obtain filter residue for standby use; 2) using chloroform for reflux extraction of the obtained filter residue, and recovering the chloroform to obtain chloroform extract; 3) using methanol to dissolve the obtained chloroform extract, performing column chromatography, eluting with methanol with every 250ml being one flow part, and merging 2-8 flow parts; 4) concentrating the merged 2-8 flow parts to dry, dissolving with methanol, using a reverse phase silica gel column for column chromatography, eluting with methanol with every 100ml being one flow part, and respectively merging 14-18 flow parts and 20-27 flow parts; and 5) concentrating the respectively merged 14-18 flow parts and 20-27 flow parts to dry, and respectively recrystallizing to obtain the two compounds of 7-hydroxy-4'-methoxy flavan and pterostilbene. The two compounds of 7-hydroxy-4 '-methoxy flavan and pterostilbene are firstly and simultaneously prepared from dracaena cochinchinensis by the method.

The invention discloses a formula of a traditional Chinese medicine capable of nourishing the stomach and tonifying the spleen. The traditional Chinese medicine is prepared from 3-5 parts of radix gentianae macrophyllae, 2-3 parts of persimmon calyx, 2-4 parts of trogopterus dung, 2-4 parts of lotus stamen, 1-4 parts of roasted fructus aurantii immaturus, 1-2 parts of charred radix et rhizoma rhei, 2-3 parts of flos caryophylli, 2-5 parts of all-grass of salicornia europaeaL., 1-2 parts of blood-flower milkweed, 1-3 parts of rhizoma cimicifugae, 1-3 parts of dracaena cochinchinensis, 1-2 parts of all-grass of salsola collina Pall., 1-2 parts of silkworm powder, 1-2 parts of radix achyranthis bidentatae and 1-3 parts of rhizoma smilacis glabrae. In this way, the traditional Chinese medicine is low in price and has the good effects of nourishing the stomach and tonifying the spleen.

Disclosed is a formula of a freckle removing product. The formula of the freckle removing product comprises, by weight, 50-70 parts of rhizoma sparganii, 15-30 parts of curcuma zedoary, 10-25 parts of pangolin scales, 5-10 parts of rhizoma cyperi, 2-8 parts of saffron, 0.2-1 part of resin of dracaena cochinchinensis, 2-5 parts of giant typhonium rhizome, 3-4 parts of red peony roots, 2-3 parts of periostracum cicadae, 1-3 parts of hirudo and 1-3 parts of bitter apricot seeds. The freckle removing product is prepared from by pure traditional Chinese medicine, can effectively remove freckles and is remarkable in effect.

The invention relates to a medicine for treating sweat stain. The medicine is prepared by taking ramulus et folium mussaendae pubescentis, flos lonicerae, dracaena cochinchinensis, evodia lepta leaf,herba hedyotidis diffusae, folium isatidis and herba corydalis saxicolae as raw materials and has a remarkable treatment effect on the sweat stain; the medicine is low in price, has no toxic and sideeffects and is safe and reliable.

The present invention discloses artificial propagation process of Dracaena cochinchinensis. The propagation process includes seedbed preparation, seed treatment, seeding, seedbed management, transplanting, field management and other steps. The present invention makes it possible for planting Dracaena cochinchinensis in large scale and protecting the wild rare medicine source plant resource.

The invention provides a Dracaena cochinchinensis callus seedling culture method. The Dracaena cochinchinensis callus seedling culture method comprises following steps: 1, explant selection; 2, induction of callus; 3, callus propagation culture; 4, callus differentiation culture, wherein 1 to 3mg / L thioridazine TDZ, 0.1 to 0.5mg / L of kinetin KT, 0.1 to 0.5mg / L of brassinolide, and 2mg / L of polyvinylpyrrolidone PVP are added into a MS differentiation culture medium; 5, rooting culture, wherein 0.5 to 1.0mg / L of naphthylacetic acid NAA and 2g / L of active carbon are added into a 1 / 2MS rooting culture medium; and 6, hardening seedling and transplanting. The Dracaena cochinchinensis multiple shoot propagation coefficient is increased to be 5 to 12 times of an original value, obtained multiple shoots are strong, rooting is easy to realize after inoculation to a rooting medium, rooting rate is 90% or higher, transplanting survival rate is 95% or higher, and a large amount of Dracaena cochinchinensis seedlings suitable for plantation can be cultured in a short time.

The invention provides a tissue culture and rapid propagation method for dracaena cochinchinensis. The tissue culture and rapid propagation method comprises the following steps: (1) taking a dracaena cochinchinensis seed as an explant and disinfecting; (2) putting the disinfected explant into an MS (Murashige and Skoog) culture medium to be induced and burgeoned to obtain a sterile test-tube plantlet; (3) putting the sterile test-tube plantlet into the MS culture medium to be subjected to test-tube plantlet rapid propagation and culture to obtain cluster buds; (4) putting the cluster buds into an MS strong seedling culture medium to carry out strong seedling culture to obtain a strong plant; (5) putting the strong plant into an MS rooting culture medium to be cultured to obtain a complete seedling with roots; and (6) taking the complete seedling with the roots and carrying out domestication; and transplanting the seedling into a sand bed to grow for one month and then transplanting the seedling to a large field. With the adoption of the culture method, the growth coefficient of the cluster buds of the obtained dracaena cochinchinensis reaches 10-15 times; the rooting rate of an obtained tissue culture seedling is more than 95% and the survival rate of a transplanted seedling bed is more than 90%, so that the large-scale seedling growth problem of the dracaena cochinchinensis is solved effectively.

The invention discloses a method of propagation by cuttings for dracaena cochinchinensis SC chen, which consists of selecting branches of dracaena cochinchinensis SC chen trees with an age of over two years, cutting the upper end of the branches, cutting the lower end to 40-50 degree of inclined surface, sealing the upper extreme tangent plane of the grafting ear with paraffin wax, immersing the lower end tangent plane in aqueous solution of root-producing powder, carrying out cuttage by the conventional process and transferring into large field planting.

The invention relates to a germchit planting method for dracaena cochinchinenisis, belonging to the plant culture technical field. The method comprises seed picking, sand bed making, seed treatment and planting as well as plant culture procedure, wherein, over ninety percent of maturity of natural seeds are selected and placed at a shadowy and ventilation place for drying after being peeled; the sand bed is made with one meter in width and five meters in length, the surrounding of the sand bed is built twenty centimeters high with bricks, inner pits are leveled, a layer of plastic film is laid and is filled with river sand of fifteen to eighteen centimeters deep, concentration of zero point five percent of potassium permanganate solution is used to sterilized the sand bed; before seeding, zero point five percent of naphthylacetic acid aqueous solution is used to immerse the seeds for two hours or thirty Celsius system of warm water is used to immerse the seeds for two to twelve hours, then two hundred to two hundred and fifty grams of seeds per square meter is tidily laid on the sand bed, one to one point five centimeters of sterilized river sand is covered on, a layer of straw is covered after through water irrigation, an arch-shaped shed is built to cover the plastic film and the shade net; twenty eight to thirty days after seeding, the seeds come out with six hundred to eight hundred plants every square meter and the rate of emergency is thirty to thirty five percent. The invention is convenient to operate and is high in seedling survival rate.