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32 results about "Dracaena cochinchinensis" patented technology

The dried resin, called xue jie (Ѫ½ß) or dragon’s blood, is used medicinally. The collection of this resin, together with habitat destruction, has made Dracaena cochinchinensis a vulnerable species in China.

Tissue culture and rapid propagation method for dracaena cochinchinensis

The invention provides a tissue culture and rapid propagation method for dracaena cochinchinensis. The tissue culture and rapid propagation method comprises the following steps: (1) taking a dracaena cochinchinensis seed as an explant and disinfecting; (2) putting the disinfected explant into an MS (Murashige and Skoog) culture medium to be induced and burgeoned to obtain a sterile test-tube plantlet; (3) putting the sterile test-tube plantlet into the MS culture medium to be subjected to test-tube plantlet rapid propagation and culture to obtain cluster buds; (4) putting the cluster buds into an MS strong seedling culture medium to carry out strong seedling culture to obtain a strong plant; (5) putting the strong plant into an MS rooting culture medium to be cultured to obtain a complete seedling with roots; and (6) taking the complete seedling with the roots and carrying out domestication; and transplanting the seedling into a sand bed to grow for one month and then transplanting the seedling to a large field. With the adoption of the culture method, the growth coefficient of the cluster buds of the obtained dracaena cochinchinensis reaches 10-15 times; the rooting rate of an obtained tissue culture seedling is more than 95% and the survival rate of a transplanted seedling bed is more than 90%, so that the large-scale seedling growth problem of the dracaena cochinchinensis is solved effectively.
Owner:GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS

Dracaena cochinchinensis planting method

The invention relates to a cultivation method for dracaena cochinchinenisis, belonging to the plant culture technical field. The planting field applied in the method is dry tropical or subtropical mountainous forest which is low in altitude and is sun-exposed. The specification of the planting pond is forty multiplies forty multiplies forty centimeter, the plant row spacing is one point five multiplies one point five meter and two hundred and ninety six plants are planted every unit of area; one to three kilograms of organic fertilizer is added and mixed evenly with the surrounding earth when the pond is backfilled to the original plane. The pond is built in a pyramid shape by piling soil based on the backfilled original plane and is twenty to twenty five centimeters over the original plane. Then the bags of the dracaena seedling are removed, with two thirds of the seedling planted on the pyramid point and pressed with soil and one third of the seedling exposed to the soil surface. The root is required to naturally stretch without winding. The exposed part does not earth up for free growing. After planting, cultivation is needed for at least two times every year. The first cultivation is around April to May and each plant is fertilized with ten to twelve granules of compound fertilizer in the market; the second cultivation is at the end of the autumn and at the beginning of the spring and each plant is fertilized with one to one point five kilograms of organic fertilizer. The invention has the advantages that the method is convenient and high in seedling survival rate.
Owner:YUNNAN DATANG HANFANG PHARMA

Application of two quadruple lactone antibiotics as anti-MRSA drugs, and extraction and separation method of two quadruple lactone antibiotics

The invention discloses an application of two quadruple lactone antibiotics as anti-MRSA drugs and an extraction and separation method of the two quadruple lactone antibiotics. According to the methoddisclosed by the invention, two quadruple lactone compounds, namely Dinactin and Trinactin, are separated from Dracaena cochinchinensis endophyte Streptomyces punctatus S009 for the first time; in-vitro anti-MRSA activity tests are carried out on the two separated compounds, and results show that the Dinactin and the Trinactin have a stronger inhibition effect on a plurality of clinically separated MRSA strains than a positive reference substance vancomycin, so that the Dinactin and the Trinactin have a better inhibition effect on multiple kinds of clinically separated MRSA strains than a positive control substance, vancomycin, and a molecular docking result shows that such kind of compounds has strong binding activity with anti-MRSA target protein PBP2a. Therefore, the invention not onlycan provide lead compounds for developing a novel anti-MRSA drug, but also can effectively protect rare and endangered medicinal plant resources, and is of great significance for reducing the damageto the diversity of wild medicinal plants.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY

Method for simultaneous preparation of 7-hydroxy-4 '-methoxy flavan and pterostilbene

The invention belongs to the field of extraction of traditional Chinese medicines, and in particular relates to a method for simultaneous preparation of 7-hydroxy-4 '-methoxy flavan and pterostilbene. The method includes the steps of: 1) taking dracaena cochinchinensis grease-containing wood or ethanol extract thereof, grinding, adding petroleum ether to extract, filtering to obtain filter residue for standby use; 2) using chloroform for reflux extraction of the obtained filter residue, and recovering the chloroform to obtain chloroform extract; 3) using methanol to dissolve the obtained chloroform extract, performing column chromatography, eluting with methanol with every 250ml being one flow part, and merging 2-8 flow parts; 4) concentrating the merged 2-8 flow parts to dry, dissolving with methanol, using a reverse phase silica gel column for column chromatography, eluting with methanol with every 100ml being one flow part, and respectively merging 14-18 flow parts and 20-27 flow parts; and 5) concentrating the respectively merged 14-18 flow parts and 20-27 flow parts to dry, and respectively recrystallizing to obtain the two compounds of 7-hydroxy-4'-methoxy flavan and pterostilbene. The two compounds of 7-hydroxy-4 '-methoxy flavan and pterostilbene are firstly and simultaneously prepared from dracaena cochinchinensis by the method.
Owner:GUILIN SANJIN PHARMACEUTICALS CO LTD

Chinese patent medicament for treating bone traumatism and preparation process thereof

The invention relates to a Chinese patent medicament for treating bone traumatism, which comprises the following components of raw materials in part by weight: 30 to 70 parts of sanchi, 5 to 20 parts of dracaena cochinchinensis, 10 to 50 parts of myrrh, 20 to 50 parts of pyrite, 20 to 50 parts of fortune's drynaria rhizome, 10 to 50 parts of dahurian angelica root and 2 to 15 parts of borneol. The preparation method comprises the following steps: putting the sanchi, the fortune's drynaria rhizome and the dahurian angelica root together, extracting the raw materials with ethanol to prepare clear ointment, decocting the produced dregs of a decoction with water, and mixing the clear ointment with the decoction to prepare fine powder; mixing the dracaena cochinchinensis, the myrrh and the pyrite to prepare fine powder, mixing the two fine powders, and adding starch and ethanol into the fine powders to prepare soft materials; and granulating the soft materials in a granulator, and filling the granules into capsules. The Chinese patent medicament has the characteristics of quick cure of bone traumatism, stable curative effect, no toxic or side effect and low cost. The preparation process has the characteristics of simple preparation and convenient popularization.
Owner:XIAN CHIHO PHARMA

A method for simultaneously preparing 7-hydroxyl-4'-methoxyflavan and pterostylbene

The invention belongs to the field of extraction of traditional Chinese medicines, and in particular relates to a method for simultaneous preparation of 7-hydroxy-4 '-methoxy flavan and pterostilbene. The method includes the steps of: 1) taking dracaena cochinchinensis grease-containing wood or ethanol extract thereof, grinding, adding petroleum ether to extract, filtering to obtain filter residue for standby use; 2) using chloroform for reflux extraction of the obtained filter residue, and recovering the chloroform to obtain chloroform extract; 3) using methanol to dissolve the obtained chloroform extract, performing column chromatography, eluting with methanol with every 250ml being one flow part, and merging 2-8 flow parts; 4) concentrating the merged 2-8 flow parts to dry, dissolving with methanol, using a reverse phase silica gel column for column chromatography, eluting with methanol with every 100ml being one flow part, and respectively merging 14-18 flow parts and 20-27 flow parts; and 5) concentrating the respectively merged 14-18 flow parts and 20-27 flow parts to dry, and respectively recrystallizing to obtain the two compounds of 7-hydroxy-4'-methoxy flavan and pterostilbene. The two compounds of 7-hydroxy-4 '-methoxy flavan and pterostilbene are firstly and simultaneously prepared from dracaena cochinchinensis by the method.
Owner:GUILIN SANJIN PHARMACEUTICALS CO LTD

Tissue culture and rapid propagation method for dracaena cochinchinensis

The invention provides a tissue culture and rapid propagation method for dracaena cochinchinensis. The tissue culture and rapid propagation method comprises the following steps: (1) taking a dracaena cochinchinensis seed as an explant and disinfecting; (2) putting the disinfected explant into an MS (Murashige and Skoog) culture medium to be induced and burgeoned to obtain a sterile test-tube plantlet; (3) putting the sterile test-tube plantlet into the MS culture medium to be subjected to test-tube plantlet rapid propagation and culture to obtain cluster buds; (4) putting the cluster buds into an MS strong seedling culture medium to carry out strong seedling culture to obtain a strong plant; (5) putting the strong plant into an MS rooting culture medium to be cultured to obtain a complete seedling with roots; and (6) taking the complete seedling with the roots and carrying out domestication; and transplanting the seedling into a sand bed to grow for one month and then transplanting the seedling to a large field. With the adoption of the culture method, the growth coefficient of the cluster buds of the obtained dracaena cochinchinensis reaches 10-15 times; the rooting rate of an obtained tissue culture seedling is more than 95% and the survival rate of a transplanted seedling bed is more than 90%, so that the large-scale seedling growth problem of the dracaena cochinchinensis is solved effectively.
Owner:GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS

Dracaena cochinchinensis seeding and cultivating method

The invention relates to a germchit planting method for dracaena cochinchinenisis, belonging to the plant culture technical field. The method comprises seed picking, sand bed making, seed treatment and planting as well as plant culture procedure, wherein, over ninety percent of maturity of natural seeds are selected and placed at a shadowy and ventilation place for drying after being peeled; the sand bed is made with one meter in width and five meters in length, the surrounding of the sand bed is built twenty centimeters high with bricks, inner pits are leveled, a layer of plastic film is laid and is filled with river sand of fifteen to eighteen centimeters deep, concentration of zero point five percent of potassium permanganate solution is used to sterilized the sand bed; before seeding, zero point five percent of naphthylacetic acid aqueous solution is used to immerse the seeds for two hours or thirty Celsius system of warm water is used to immerse the seeds for two to twelve hours, then two hundred to two hundred and fifty grams of seeds per square meter is tidily laid on the sand bed, one to one point five centimeters of sterilized river sand is covered on, a layer of straw is covered after through water irrigation, an arch-shaped shed is built to cover the plastic film and the shade net; twenty eight to thirty days after seeding, the seeds come out with six hundred to eight hundred plants every square meter and the rate of emergency is thirty to thirty five percent. The invention is convenient to operate and is high in seedling survival rate.
Owner:YUNNAN DATANG HANFANG PHARMA
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