A kind of dracaena extract with antibacterial function and its preparation and application

A technology of Dracaena glabra and its extract, which is applied in the field of dracaena phoenix leaf extract, can solve the problem of no antibacterial agent being found, and achieve the effect of broad-spectrum inhibitory activity

Active Publication Date: 2022-03-11
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the preparation of bacteriostatic agents using stilbene compounds

Method used

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  • A kind of dracaena extract with antibacterial function and its preparation and application
  • A kind of dracaena extract with antibacterial function and its preparation and application
  • A kind of dracaena extract with antibacterial function and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of embodiment 1 Dracaena glabra resin total extract:

[0035] Take 10.5 g of the resin of Dracaena glabrata, extract it three times with methanol (3×0.3 L), concentrate the methanol and evaporate to dryness to obtain 1.8 g of the total resin extract of Dracaena glabrata. TLC analysis found that the main substances in the total resin extract were stilbene compounds.

Embodiment 2

[0036] The separation and identification of embodiment 2 stilbene compounds I, II, III:

[0037] Weigh 1.5g of the total resin extract, use MCI column chromatography for rough separation, and sequentially elute with methanol / water (2:8, 4:6, 6:4, 8:2, V / V) to obtain 4 Fractions (fractions F1-F4), in which F3 was passed through a silica gel column (dichloromethane / acetone, 4:1, v / v), were further separated to obtain fractions F3a and F3b. Subfraction F3a was chromatographically separated on a Sephadex LH-20 column (acetone was the elution solvent), and finally separated by reverse-phase semi-preparative HPLC (42% methanol water; 3mL / min; Eclipse XDB-C 18 column, 5 μm, 9.4×250mm) to obtain compound I and compound II. Fraction F3b was separated by column chromatography on a Sephadex LH-20 column (acetone was the elution solvent) to obtain compound III. The chemical structures of compounds I, II, and III were resolved by NMR data (Table 1) combined with mass spectrometry analys...

Embodiment 3 Embodiment 1

[0042] The antibacterial activity detection of embodiment 3 embodiment 1 gained dracaena phoenix leaf resin total extract:

[0043]The antibacterial activity of the resin extract of Dracaena phoenix leaf against tobacco black shank, Fusarium graminearum, maize leaf spot, maize leaf spot and Campylobacter crescentus was determined by selective antibacterial activity assay method. The antibacterial activity of the total extract of Dracaena glabra resin was tested by double dilution method. Strains were grown on PDA at 28°C for one week. The fungal spores on the plate were eluted with potato dextrose broth (PDB) so that the spore concentration of the eluate was 10 5 individual / mL. 100 μL of each spore suspension was then added to the 96-well plate. Add 2 μL of DMSO-dissolved extract, the extract concentration ranges from 4 to 256 μg / mL; at the same time, DMSO solvent is used as a negative control, and nystatin is used as a positive control. After 48 hours using an incubation ...

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PUM

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Abstract

The invention relates to an extract of Dracaena glabrata with antibacterial function, in particular to the application of the extract and its stilbene monomer compound as a bacteriostatic agent for inhibiting plant pathogenic bacteria. A kind of dracaena extract with antibacterial function, described dracaena extract is the extract that extracts from the resin of dracaena, and the extract contains the following chemical formula: The stilbene compounds shown in I, II, and III, the total resin extract and the stilbene compounds of the present invention have broad-spectrum inhibitory activity on plant pathogenic fungi, and the inhibitory concentration ranges between 3.27-46.38 μg / mL.

Description

technical field [0001] The invention relates to an extract of Dracaena glabrata with antibacterial function, in particular to the application of the extract and its stilbene monomer compound as a bacteriostatic agent for inhibiting plant pathogenic bacteria. Background technique [0002] Resins can be divided into natural resins and artificial resins. Natural resins are solid or semi-solid substances formed by the oxidation of terpenoids exuded from plants by air, and are insoluble in water. The present invention is extracted from the resin secreted by the dracaena dracaena. The bark of the dracaena dracaena will secrete dark red juice after being cut. Blood stasis, analgesia and hemostasis and other effects have excellent development and utilization prospects. [0003] Pesticides play a very important role in agricultural production. With the improvement of people's health awareness and higher requirements for the quality of agricultural products, the market has higher and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C27/26C07C37/68C07C37/82C07C39/21C07C41/36C07C43/23A01N31/16A01P3/00
CPCC07C41/36C07C37/685C07C37/82A01N31/16C07C43/23C07C39/21
Inventor 骆世洪牛生吏华娟
Owner SHENYANG AGRI UNIV
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