1611 results about "Broad spectrum" patented technology

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention discloses a broad-spectrum lytic bacteriophage for preventing and controlling various vibrio infections in an aquaculture environment. The invention relates to V.alginolyticus phage vBValM-R37J, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on August 6, 2025, the address is No.100 Courtyard, Xianlie Middle Road, Xianlie District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO.66813-B1. The invention further discloses a method for preparing the V.alginolyticus phage vBValM-R37J strain. The bacteriophage R37J is a candidate therapeutic bacteriophage with wide host spectrum, high splitting efficiency, gene safety and environmental stability, and has the potential of being popularized and applied in aquaculture as a novel biological tool for accurately preventing and controlling various vibrio infections. A new biological resource is provided for accurate prevention and control of aquatic pathogenic bacteria, and a theoretical basis and a technical support are provided for popularization and application of a phage therapy in an actual culture system.

The invention relates to the technical field of biomedicine, in particular to a primer probe combination for broad-spectrum screening of common respiratory pathogens and application of the primer probe combination. Through screening and optimization, the respiratory pathogen nucleic acid detection primer probe combination for broad-spectrum screening of common acute respiratory infection and community-acquired pneumonia is obtained; the primer probe combination covers 18 respiratory tract pathogens, has the advantages of high accuracy, good specificity, strong anti-interference capability, good inclusiveness, high sensitivity, hyperjoint detection and the like, can realize parallel detection of 20 targets in cooperation with totally-closed single-tube PCR amplification, greatly improves early diagnosis of respiratory tract diseases with complex pathogen structures, and has wide application prospects. The method has very important significance on prevention, control and treatment of infectious diseases.

The invention discloses an antibacterial peptide based on D-type amino acid and application of the antibacterial peptide, and the antibacterial peptide is characterized in that the amino acid sequence is w-r-r-w-r-r-w-w-r-k-r (dTrp-dArg-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dLys-dArg). The antibacterial peptide can be synthesized through an Fmoc solid-phase chemical method, the synthesis difficulty is low, and the in-vivo antibacterial activity is good. The antibacterial peptide especially has broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus, is low in hemolytic toxicity, can reach half or more of cell survival rate under medium and low concentration, almost has no toxic effect on cells, and can be used for preparing the antibacterial peptide with a broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus. The stability is good, the operability is high, and the cost is low.

The invention discloses application of a small molecule compound in prevention and treatment of plant RNA virus infection, and belongs to the technical field of biology. According to the invention, 2 '-amino-2'-deoxyguanosine (ADG) is used as a unique antiviral active component to be applied to prevention and treatment of plant RNA viruses for the first time, and broad-spectrum inhibition of various plant RNA viruses such as turnip mosaic virus (TuMV), tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV) can be realized only by a low concentration of 250 [mu] M and a simple exogenous spraying mode; and after spraying, the normal growth of plants is not influenced, the risk of phytotoxicity is thoroughly avoided, the safety, high efficiency and broad spectrum of plant RNA virus prevention and control are remarkably improved, and important practical application significance is achieved for guaranteeing the yield and quality of crops in agricultural production and promoting agricultural green transformation.

The invention discloses a dual-mechanism antibacterial photosensitizer as well as a preparation method and application thereof, and relates to the technical field of biomedical photoelectric functional materials. The invention relates to a triphenylamine derivative, which can realize fluorescence imaging of bacterial or fungal infection by utilizing fluorescence characteristics, can generate active oxygen under illumination to play a photodynamic therapy role, is suitable for preparing photodynamic therapy preparations and developing antibacterial materials, and can also be applied to the fields of environment and public health and tumor treatment. The problems that an existing photosensitizer is insufficient in broad spectrum, different in fungus drug resistance mechanism, limited in single treatment effect and the like can be effectively solved, and the photosensitizer has wide application prospects.

The invention provides a super-hydrophobic passion fruit peel carbon aerogel VOCs adsorbent based on low-temperature plasma synergistic nitrogen doping modification and a preparation method thereof, and relates to the technical field of VOCs adsorbents. The super-hydrophobic passion fruit peel carbon aerogel VOCs adsorbent based on low-temperature plasma synergistic nitrogen doping modification is prepared by crushing passion fruit peel, mixing the crushed passion fruit peel with a nitrogen-containing compound to form gel, performing low-temperature plasma modification, performing freeze drying, performing high-temperature carbonization, and performing water washing and drying. The adsorbent is spherical, and the water contact angle is 115.8 degrees to 152.4 degrees. The adsorbent disclosed by the invention has the advantages of super hydrophobicity, high specific surface area, developed microporous structure, excellent VOCs adsorption performance, broad-spectrum adsorption capacity, good reusability and mechanical strength, the preparation method is simple in process, green and environment-friendly, low in cost and good in universality, a new technical scheme is provided for VOCs treatment in a high-humidity environment, and the adsorbent has a good application prospect.

The invention discloses silencing and mildew-proof organic silica gel as well as a preparation method and application thereof, and belongs to the technical field of organic silicon binding material polymers. Comprising the following components in parts by weight: 35-45 parts of nano calcium carbonate, 32-40 parts of hydroxyl-terminated polysiloxane, 5-9 parts of silencing powder, 0.5-2.5 parts of glass fiber powder, 5-9 parts of a hyperbranched polymer, 2-5 parts of a hydrophobic surface modifier, 1.5-3 parts of color paste, 1-2 parts of a water-based coupling agent, 1-2 parts of a cross-linking agent, 0.5-2.5 parts of a mildew preventive, 0.4-2 parts of a composite titanide and 0.4-0.7 part of nano silver powder. According to the invention, the nano silver powder and the mildew preventive are compounded, and an inorganic sterilization system is combined, so that broad-spectrum environment-friendly bacteriostasis is realized, and high-humidity long-acting protection is met; the powder is subjected to hydrophobic treatment, moisture is blocked, and high adhesion is kept; silencing powder serves as a core, a sound absorption network is cooperatively formed, the noise reduction capacity is improved, and the device is suitable for multiple scenes.

The invention provides a compound disinfectant for preventing and treating aquaculture virus diseases and a preparation method thereof. The composite disinfectant is prepared by introducing polyhexamethylene guanidine or a salt thereof, a quaternary ammonium salt compound, metal nanoparticles and an auxiliary agent into a water system as main raw materials, and is prepared by the following steps: adding a quaternary ammonium salt compound solution and a penetrating agent into a polyhexamethylene guanidine or salt solution thereof, and uniformly stirring and mixing to obtain a mixed solution A; slowly dropwise adding the metal nanoparticle dispersion liquid into the mixed solution A, starting ultrasonic treatment at the same time, and uniformly stirring and mixing to obtain a mixed solution B; dropwise adding the pH regulator solution into the mixed solution B, regulating the pH, adding the preservative solution, supplementing water, uniformly mixing, standing and curing to obtain the composite disinfectant. The compound disinfectant aims at achieving synergistic interaction, finally achieves the comprehensive prevention and treatment effects of being efficient, low in toxicity, broad in spectrum and not prone to inducing drug resistance, and particularly achieves efficient and safe prevention and control on fish, shrimp and crab virus diseases so as to meet the urgent requirement for healthy aquaculture.

The invention relates to the technical field of biology, in particular to application of a rice OsPBL8 gene in enhancing resistance of rice to magnaporthe oryzae. The rice OsPBL8 gene is a rice endogenous gene participating in the processes of plant growth and development, abiotic stress response, hormone signal channel regulation and control and the like. The invention provides application of the OsPBL8 gene in the field of disease resistance, the OsPBL8 gene is overexpressed through gene editing, and the expression quantity of receptor-like cytoplasm kinase OsPBL8 in plants is increased, so that a plurality of signal channels are regulated and controlled, and the germ resistance of the plants is synergistically improved. By adjusting key hub nodes of plant targeted immune signal transduction, more lasting broad-spectrum disease resistance can be provided, good universality is achieved, disease resistance and growth can be balanced, good practicability is achieved, and improvement of disease resistance of various rice varieties is facilitated.

The invention discloses an application of an antibacterial peptide and an application of the antibacterial peptide in preparation of antibacterial drugs for treating carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae (ATCC700603), escherichia coli (ATCC25922), staphylococcus aureus and methicillin-resistant staphylococcus aureus, the antibacterial peptide is characterized in that the sequence is w-r-r-w-k-r-w-w-r-r, and the sequence is shown in the description. All amino acids are D-type amino acids; the antibacterial peptide can be used as an antibacterial substance in wash supplies, food preservative additives, medical consumable antibacterial agents and cosmetics, can be synthesized through an Fmoc solid-phase chemical method, and is low in synthesis difficulty and good in in-vivo antibacterial activity. Particularly, the antibacterial peptide has broad-spectrum killing activity against carbapenem pseudomonas aeruginosa, klebsiella pneumoniae (ATCC700603), escherichia coli (ATCC25922), staphylococcus aureus and methicillin-resistant staphylococcus aureus, the cell survival rate of the antibacterial peptide within the range of medium and low concentration (2 mu g / mL-32 mu g / mL) reaches half or more, the toxicity is small, the toxicity is almost avoided, the operability is high, and the antibacterial peptide can be widely applied to the field of medical application. The cost is low.

The invention discloses two mycobacterium bacteriophages and application thereof. The two bacteriophages both have a splitting spectrum width and efficient splitting capability, can specifically realize effective splitting of mycobacteria of five specific sequence types, and are further applied to the field of prevention and control of mycobacteria. Specifically, the bacteriophage MP2435 and the bacteriophage MP2451 not only can effectively inhibit the proliferation process of mycobacteria and block the formation of a biofilm of the mycobacteria, but also can efficiently kill the mycobacteria in a milk matrix; meanwhile, the two bacteriophages can still keep relatively high activity under the conditions that the temperature is 37-65 DEG C and the pH value is 3-12, and the bactericidal activity of the bacteriophages can be stably maintained even in acid-base, high-temperature and ultraviolet-containing extreme environments. Through the characteristics, the application scene and application range of the bacteriophage in mycobacterium prevention and control are remarkably widened, and an efficient and stable novel technical means is provided for biological prevention and control of mycobacterium.

The present invention provides a rabies virus G protein antigen epitope targeting completely human monoclonal antibody and applications thereof, the completely human monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region has three complementary determining region amino acid sequences: GDISSSCFY, IHYSGST and ARHRRGYCYDSEKGGTNWFDP, and the light chain variable region has three complementary determining region amino acid sequences: GDISSSCFY, IHYSGST and ARHRRGYCYDSEKGGTNWFDP. The amino acid sequences of the three complementary determining regions of the light chain variable region are respectively as follows: QGISND, ATS and LQDYEFPLT. The fully human monoclonal antibody has efficient and broad-spectrum anti-rabies virus neutralizing activity, is high in expression, fully human-derived and good in stability, and can be used for preparing rabies virus detection products or drugs for preventing and treating rabies.

The invention discloses streptomyces griseorubens and an application of the streptomyces griseorubens in phthalate pollution remediation. The bacterial strain is Streptomyces griseorubens FZ202, the preservation number of the bacterial strain is GDMCC No: 61372, the bacterial strain has good environmental adaptability and efficient degradation capacity, DEHP can be effectively degraded within 100-800 mg / L, the highest degradation efficiency can reach 94.1%, and complete degradation can be achieved. The strain is inoculated into PAEs contaminated soil and interacts with rice, DEHP residues in the soil and crop bodies can be effectively reduced, various PAEs can be synergistically degraded, and the strain has the broad-spectrum degradation characteristic. Meanwhile, through multiple mechanisms of degrading pollutants, promoting plant growth, improving the soil environment and regulating and controlling microbial communities, the strain remarkably improves the growth performance of rice and the self-cleaning capacity of soil, and has a wide agricultural application prospect.

The invention relates to bacillus for producing lipopeptide, bacteriostatic lipopeptide of the bacillus and application of the bacillus to aquatic products, and belongs to the technical field of microorganisms. The bacillus velezensis is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063, and the bacillus velezensis strain FJAT-57093 is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063. The bacteriostatic lipopeptide is prepared from three types of lipopeptides, namely, iturin, fengycin and surfactin. The invention further discloses a preparation method of the bacteriostatic lipopeptide. The bacillus and the bacteriostatic lipopeptide have a broad-spectrum bacteriostatic effect on aquatic pathogenic bacteria, can be used for aquaculture, and have a wide application prospect.

The electro-catalytic oxidation treatment device comprises an electro-catalytic box, a plurality of water pipe connectors are arranged at one length end of the electro-catalytic box, a supporting plate is arranged at the bottom in the electro-catalytic box, a plurality of polar plate groups are arranged on the upper portion of the supporting plate in the length direction, and insulating bars penetrate through the polar plate groups; the polar plate group comprises two cathode plates and an anode plate, the anode plate is positioned in a gap between the two cathode plates and is parallel to the two cathode plates, the bottoms of the anode plate and the two cathode plates are fixedly connected, and the anode plate and the cathode plate are L-shaped and form the right-angle U-shaped polar plate group; the upper ends of the anode plates and the cathode plates are fixedly connected with metal rods, the metal rods penetrate out of the side walls of the adjacent electro-catalysis boxes, and electric connectors are arranged at the outer ends of the metal rods. According to the electrocatalytic oxidation treatment device for the landfill leachate, the generation amount of oxidants such as hydroxyl radicals is changed by adjusting the current and voltage of the catalytic polar plate, so that pollutants are oxidized and removed. The method has the characteristics of simplicity in operation, low cost, broad spectrum, full quantization and the like.

The invention belongs to the technical field of fine chemical engineering, and particularly discloses a method for preparing a broad-spectrum bactericide 2-n-butylbenzo [d] isothiazole-3 (2H)-ketone by a molecular editing method. The preparation method comprises the following specific steps: taking 3H-1, 2-benzodisulfophenol-3-ketone and n-butylamine as raw materials, taking Selectfluor as an additive, adding the raw materials and the additive into a mixed solvent, and carrying out heating and stirring reaction, so as to obtain the target product 2-n-butylbenzo [d] isothiazole-3 (2H)-ketone (BBIT). The method is simple and convenient to operate, the raw materials are easy to obtain, the yield is high, thiophenol raw materials or excessive alkali are prevented from being used, and potential application value is achieved.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody combination for detecting porcine epidemic diarrhea virus (PEDV) S protein and its application. The combination comprises monoclonal antibodies 4E11 and 1F3. The heavy chain CDR1-3 (SEQ ID NO. 1-3) and light chain CDR1-3 (SEQ ID NO. 4-6) of 4E11, and the heavy chain CDR1-3 (SEQ ID NO. 7-9) and light chain CDR1-3 (SEQ ID NO. 10-12) of 1F3, together impart high specificity and affinity. Detection methods such as double-antibody sandwich ELISA constructed based on this combination, using 4E11 as the coating antibody and 1F3 as the labeling antibody, have advantages such as high sensitivity, strong specificity, and broad spectrum, and can be used for in vitro detection of PEDV S protein, biological sample analysis, and quality control of biological products.

The invention belongs to the technical field of biosensors, and provides a tetracycline ultra-sensitive detection method based on a CdTe (at) PVP (at) ZIF-8 core-shell material and a cascade amplification sensor. The sensor comprises an Apt-T composite probe, an M-2P chain, a complementary chain C, a self-locking DNAzyme hairpin chain and a hairpin chain H, the Apt-T composite probe is obtained by hybridizing an aptamer Apt and a T chain; the nucleotide sequences of the Apt, the T chain, the template chain M, the enriched chain P, the complementary chain C, the DNAzyme chain and the H chain are as shown in SEQ ID NO: 1-7. The sensor disclosed by the invention has the advantages of broad-spectrum recognition, high specificity, high detection speed, simplicity in operation, low detection limit and the like, can make up for the defects and deficiencies of an existing detection method for tetracycline antibiotics, realizes rapid and accurate ultra-sensitive detection on the tetracycline antibiotics, and has potential application prospects in food safety and environment detection.

The invention provides a fluorenyl alcohol derivative which can be used as an antibiotic adjuvant with a broad-spectrum synergistic effect. According to the compound, bacteria outer membrane asymmetric protein MlaC and phospholipase A1 PldA are taken as targets, and a series of fluorenyl alcohol derivatives targeting the bacteria outer membrane asymmetric protein MlaC and phospholipase A1 PldA are obtained through screening. The preferable compound WTU-15 can improve the antibacterial activity of antibiotics such as polymyxin, cefepime, tetracycline and the like on negative bacteria such as sensitive and drug-resistant escherichia coli, salmonella, klebsiella pneumoniae, acinetobacter baumannii and the like, and can also improve the in-vivo treatment effect of cefepime on mice infected by the drug-resistant acinetobacter baumannii. And the visceral organs of the mice survived after administration have fewer bacteria, and the mice are better after being healed. Normal physiological and visceral organ functions of the mouse are not affected by single and continuous administration of the WTU-15.

The invention relates to the field of microorganisms, in particular to lactobacillus plantarum TRT-01 with anti-inflammatory and bacteriostatic functions as well as a preparation method and application of the lactobacillus plantarum TRT-01, the lactobacillus plantarum TRT-01 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32237, and the preservation date is October 17, 2024. The plant lactobacillus has broad-spectrum bacteriostatic activity on 10 pathogenic bacteria such as helicobacter pylori, streptococcus mutans and the like, and can regulate Th1 / Treg immune balance and relieve inflammatory response by inducing dendritic cells to secrete cell factors IL-10 and IL-12, inducing T cells to secrete cell factors TGF-beta and IFN-gamma and inhibiting secretion of cell factors IL-6 at the same time. Therefore, the plant lactobacillus TRT-01 disclosed by the invention can be used for preparing a composition with anti-inflammatory and bacteriostatic effects, is suitable for the fields of medicines, foods, health-care products and the like, and is particularly suitable for developing a microbial preparation with a probiotic effect.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting porcine epidemic diarrhea virus S protein and application of the monoclonal antibody combination. The combination comprises monoclonal antibodies 4E11 and 1F3, a heavy chain CDR1-3 (SEQ ID NO.1-3) and a light chain CDR1-3 (SEQ ID NO.4-6) of the 4E11, and a heavy chain CDR1-3 (SEQ ID NO.7-9) and a light chain CDR1-3 (SEQ ID NO.10-12) of the 1F3 jointly endow the combination with high specificity and affinity. The double-antibody sandwich ELISA and other detection methods constructed based on the combination take 4E11 as a coating antibody and 1F3 as a labeled antibody, have the advantages of high sensitivity, strong specificity, good broad spectrum and the like, and can be used for in-vitro detection of PEDV S protein, biological sample analysis and quality control of biological products.

The invention relates to the technical field of biology, in particular to application of an OgRKa gene and an upstream promoter thereof to improvement of rice bacterial blight and rice blast resistance, nucleotide sequences of the OgRKa gene and the upstream promoter are as shown in SEQ ID No.1, a CDS sequence is as shown in SEQ ID No.4, and an amino acid sequence of an encoding protein OgRKa is as shown in SEQ ID No.5. The homologous gene OgRKa of the rice MRKa gene (a rice plant over-expressing the MRKa gene has partial resistance to the rice bacterial blight, and a rice plant expressing the MRKa gene by using an MRKa self promoter has no resistance to the rice bacterial blight) is homologous cloned from oryza meyeriana through PCR (Polymerase Chain Reaction) amplification. Compared with the prior art, the resistance of rice plants with the OgRKa gene overexpressed by the 35S promoter or the OgRKa gene expressed by the promoter per se to a plurality of xanthomonas oryzae and magnaporthe oryzae is remarkably enhanced, the approximate immune level is achieved, the broad-spectrum high-resistance xanthomonas oryzae and magnaporthe oryzae are shown, and good application prospects are achieved in the aspect of breeding of xanthomonas oryzae and magnaporthe oryzae resistant varieties.

The invention belongs to the technical field of microbial antibacterial preparations, and particularly discloses a novel strong vibrio parahaemolyticus bacteriophage isolated strain vBVpSCC6 and application of the novel strong vibrio parahaemolyticus bacteriophage isolated strain vBVpSCC6. The bacteriophage vBVpSCC6 is preserved in the Guangdong Microbial Culture Collection Center on September 14, 2020, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 61189-B1. The bacteriophage has good tolerance to environments such as temperature, pH, ultraviolet rays and the like, and has a good prevention and control effect on aquatic product pollution caused by vibrio parahaemolyticus. The bacteriophage has a lysis effect on multi-drug-resistant vibrio parahaemolyticus, can replace antibiotics to play a bacteriostatic role, effectively solves the problem of drug resistance faced by current antibiotic treatment, and has relatively good utilization value and application prospect. The bacteriophage can also be prepared into a composition with other bacteriophages to play a synergistic effect, and the bacteriophage can be applied to wider-spectrum antibacterial or bacteriostatic application.

The invention relates to the technical field of medicines, and discloses an application of a traditional Chinese medicine composition in a humanized flora transplantation model.The application comprises the following steps that an experimental body is pretreated through sterile feeding or broad-spectrum antibiotics, then coprophilous fungus suspension of a functional dyspepsia patient is transplanted, and the humanized flora transplantation model is constructed; acquiring physiological data of the humanized flora transplantation model, and performing intervention on the traditional Chinese medicine composition after the humanized flora transplantation model is completed; acquiring physiological data of the traditional Chinese medicine composition intervened humanized flora transplantation model, and analyzing an evaluation result of the traditional Chinese medicine composition intervened humanized flora transplantation model by combining the evaluation parameters of the humanized flora transplantation model. The bottleneck that the traditional animal model is greatly different from the human microflora and the curative effect and the mechanism are difficult to transform is overcome, so that the research result is closer to the clinical practice, and the molecular mechanism of the traditional Chinese medicine composition is reliably revealed.

The invention belongs to the technical field of microorganisms, and discloses a riemerella anatipestifer bacteriophage PT56, a bacteriophage composition thereof and application of the riemerella anatipestifer bacteriophage PT56, the collection number of the riemerella anatipestifer bacteriophage PT56 is CGMCCNO.46492, and the riemerella anatipestifer bacteriophage PT56 is collected in China General Microbiological Culture Collection Center on May 26, 2025, and the collection number is CGMCCNO.46492. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The riemerella anatipestifer bacteriophage PT56 has broad-spectrum bactericidal ability on riemerella anatipestifer of different serotypes, has certain temperature stability and acid-base stability, and can be used as an active ingredient for preparing drugs, disinfectants, kits and the like for preventing and treating riemerella anatipestifer diseases. The riemerella anatipestifer disinfection solution is widely applied to poultry diseases caused by riemerella anatipestifer and disinfection and sterilization of each link in the product production and processing process, is safe to use, and effectively reduces the incidence rate and the death rate of riemerella anatipestifer diseases such as poultry infectious serositis and the like.

The invention discloses application of a broad-spectrum influenza virus polypeptide vaccine in preparation of a medicine for preventing influenza virus infection and controlling influenza virus reinfection, and belongs to the technical field of biological medicine. The vaccine is designed on the basis of artificially synthesized polypeptide, a polypeptide sequence which is highly conservative and has immunocompetence is screened and optimized aiming at various subtype influenza virus proteins, a broad-spectrum polypeptide vaccine P125-H is constructed, and an Ii-Key technology and an MF59 adjuvant are applied to enhance immune response. The vaccine can effectively induce immune protection against H1N1 and H7N9 influenza A viruses, significantly reduce the virus load, reduce pathological injury to the lung and improve the survival rate. The P125-H vaccine is composed of three optimized polypeptides, has a cross protection effect on various influenza virus subtypes, has the potential of being developed into a broad-spectrum influenza vaccine, and provides a new technical scheme for coping with influenza virus variation and improving vaccine efficacy.

The invention discloses bacillus tequilensis 83IBC04-2 and application thereof, the bacillus tequilensis 83IBC04-2 comes from a deep sea hydrothermal zone and is preserved in China Center for Type Culture Collection on October 15, 2025, and the preservation number is CCTCC NO: M20252212. The compound has a remarkable antagonistic effect on a plurality of important pathogenic fungi such as tomato gray mold, tomato early blight, rice blast and wheat scab, can be used for preparing a broad-spectrum and high-efficiency biological bactericide, effectively reduces the use amount of chemical pesticides, and reduces pesticide residues and environmental pollution.

The invention relates to the technical field of fruit and vegetable preservation, in particular to a calcium ion reinforced bacillus subtilis fermentation preservative and application thereof in fresh-cut vegetables. The preparation method of the calcium ion reinforced bacillus subtilis fermentation liquor comprises the following steps: collecting the bacillus subtilis fermentation liquor cultured for 120 hours, weighing a certain mass of calcium salt according to the volume of the fermentation liquor, adding the calcium salt into the fermentation liquor, quickly stirring until the calcium salt is dissolved, sealing, reacting at constant temperature for a certain time, centrifuging, collecting supernate, and drying to obtain the calcium ion reinforced bacillus subtilis fermentation liquor. And freezing and drying the supernate to obtain the antibacterial preservative. The calcium ion reinforced bacillus subtilis fermentation liquor prepared by the invention has broad-spectrum and efficient antibacterial performance, can effectively maintain the fresh sensory quality of fresh-cut radishes and fresh-cut cabbages, effectively delays the quality aging and deterioration of the fresh-cut radishes and fresh-cut cabbages, and achieves the purpose of prolonging the shelf life of fresh-cut vegetables. The preservative is suitable for preservation of fruits, vegetables and fresh-cut vegetables, solves the problems of narrow antibacterial spectrum and short action time of fermentation liquor, and has a good application effect.