2816 results about "Broad spectrum" patented technology

Provided are a small-molecule antibacterial polypeptide, and a preparation method therefor and a use thereof. The antibacterial peptide provided has stronger antibacterial activity than most other derivative polypeptides and antibacterial polypeptides disclosed in existing reports. Multiple strains known for drug resistance are used to carry out bactericidal activity evaluation. The result shows that the antibacterial peptide provided has broad-spectrum antibacterial activity against drug-resistant strains, achieves more comprehensive antibacterial effects, and has a wider range of indications.

The invention discloses bacillus velezensis GX0003742 and biocontrol application thereof, and belongs to the technical field of microorganism application. The invention discloses bacillus velezensis GX0003742 with a broad-spectrum bacteriostatic effect, which is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 14, 2024, and the preservation number is CGMCC No.31981. The bacillus velezensis GX0003742 has the advantages that the bacillus velezensis GX0003742 can be used for preparing the bacillus velezensis GX0003742; the strain can obviously inhibit growth of colletotrichum basjoo mycelia and obviously reduce occurrence of postharvest diseases of bananas, has an inhibiting effect on various pathogenic bacteria, has a good insecticidal effect on auricular bean root-knot nematode, and provides a new strain resource for biological prevention and control of plant diseases.

The invention relates to bacillus velezensis SMB1 and application thereof in prevention and treatment of plant diseases, and belongs to the technical field of biological prevention and treatment of plant diseases. The strain is preserved in the China Center for Type Culture Collection on November 27, 2024, the preservation address is Luojia mountain, Luojia mountain, eight road, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M20242647. The bacillus velezensis SMB1 provided by the invention has a remarkable bacteriostatic effect on plant pathogenic fungi, plant pathogenic oomycetes, plant pathogenic bacteria and animal pathogenic bacteria, has an extremely good broad-spectrum bacteriostatic function, has a great application potential in biological control of plant diseases, can reduce the use of chemical pesticides, and has a good application prospect. The pesticide residue in agricultural products is reduced, the environment is protected, and the urgent demand on pollution-free agricultural product production at present is met.

The invention discloses a bovine serum albumin combined nano antibody and a preparation method and application thereof, the amino acid sequence of the bovine serum albumin combined nano antibody Nb1 is shown as SEQ.ID No.1, the nano antibody Nb not only has high affinity to bovine serum albumin, but also has good binding activity to both human serum albumin and mouse serum albumin, and the nano antibody Nb has high affinity to human serum albumin and mouse serum albumin. And the antibody has a relatively good broad-spectrum application range, and can be widely applied to the aspects of drug half-life period enhancement, tumor targeted therapy and imaging, immunodetection or diagnosis, recombinant albumin purification or enrichment and the like.

The invention relates to bacillus, a biocontrol inoculant prepared from the bacillus and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as ZZ1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the preservation center is numbered as CGMCC No.31374, and the preservation time is July 19, 2024. The bacillus ZZ1 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus ZZ1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention discloses a polypeptide and application thereof in preparation of a medicine for preventing and / or treating cancers. The amino acid sequence of the polypeptide is shown as SEQ ID No. 1 or SEQ ID No. 2. The cancer is at least one of breast cancer, colorectal cancer, leukemia, esophageal cancer, pancreatic cancer, brain glioma, kidney cancer, skin cancer, osteosarcoma, ovarian cancer and prostate cancer. The invention designs and synthesizes the polypeptide of a targeted staphylococcus tumor protein compound SND1-MTDH containing a nuclease structural domain 1 (SND1 and a heteromucin (MTDH), finds that the polypeptide has excellent inhibitory activity on various different types of human cancer cells for the first time, and provides an optional scheme for broad-spectrum anti-cancer drugs. The polypeptide applied in the invention is simple in synthesis process and low in production cost.

The invention provides a broad-spectrum antibacterial peptide, an antibacterial agent and application, and belongs to the technical field of biological medicine. The invention provides a broad-spectrum antibacterial peptide. The broad-spectrum antibacterial peptide comprises an amino acid sequence as shown in SEQ ID No. 1. The minimum inhibitory concentration of the antibacterial peptide to gram-positive bacteria, gram-negative bacteria and fungi proves that the antibacterial peptide has obvious antibacterial activity to the gram-positive bacteria, the gram-negative bacteria and the fungi. The invention also verifies the minimum inhibitory concentration of the antibacterial peptide to clinical drug-resistant bacteria. The cytotoxicity of the SL-12 disclosed by the invention is relatively weak, the SL-12 only shows obvious cytotoxicity at 200 [mu] g / ml, and the minimum inhibitory concentration of the SL-12 is 6.25-12.5 [mu] g / ml; the SL-12 has no obvious hemolytic activity when the concentration is 100 [mu] g / ml or lower, and is high in safety.

The invention discloses a plant lactobacillus with a bacteriostatic effect and application of the plant lactobacillus. The lactobacillus plantarum is named as lactobacillus plantarum C07, the preservation number is CCTCC NO: M20242086, the preservation unit is China Center for Type Culture Collection, the preservation time is September 26, 2024, and the preservation address is Wuhan University, Wuhan University, No. 299, eight road, Wuchang District, Wuhan City, Hubei Province. The strain can produce lactic acid and indole-3-lactic acid at high yield, has a very strong antibacterial effect on multiple putrefying and pathogenic microorganisms such as staphylococcus aureus, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella and mould, and is a broad-spectrum antibacterial microorganism.

The invention discloses a broad-spectrum biocontrol strain water bacillus barren ZG18 and application thereof, and belongs to the technical field of plant disease control, and the broad-spectrum biocontrol strain water bacillus barren ZG18 is characterized in that the water bacillus barren ZG18 is water bacillus barren and is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the water bacillus barren ZG18 is CGMCC (China General Microbiological Culture Collection Center). The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China; the preservation date is September 6, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The biocontrol bacterium strain disclosed by the invention has a relatively strong inhibition effect on pathogenic fusarium sporotrichum, fusarium graminearum, botrytis cinerea, rhizoctonia solani and corticosis cucurbitae, the prevention and treatment effect of the strain ZG18 on tobacco target leaf spot reaches up to 51.34%, the prevention and treatment effect of the strain ZG18 on tobacco damping-off in a pot experiment is 65.91%, and the strain ZG18 has a promoting effect on tobacco growth.

The invention relates to the field of microorganisms, in particular to a plant lactobacillus and application thereof in bacteriostasis. Specifically, the preservation number of the plant lactobacillus LP06 is CGMCC (China General Microbiological Culture Collection Center) No. 28412. The plant lactobacillus LP06 provided by the invention can be used for inhibiting the growth of debaryomyces hansenii, candida tropicalis and penicillium rochei, and also has an inhibiting effect on super bacteria acinetobacter baumannii, pseudomonas aeruginosa, klebsiella, escherichia coli, staphylococcus aureus and listeria monocytogenes. The plant lactobacillus LP06 provided by the invention has broad-spectrum bacteriostatic ability, and bacteriostatic powder prepared from the bacterium can be used as a biological preservative for corrosion prevention of dairy products such as yoghourt, cheese and the like and other foods.

The present disclosure relates to the technical field of antibacterial drugs, and specifically relates to a micromolecular antibacterial polypeptide, and a preparation method therefor and the use thereof. The antibacterial peptide provided in the present disclosure has a stronger antibacterial activity compared with most other derived polypeptides and the antibacterial polypeptides disclosed in the existing reports. Bactericidal activity evaluation is carried out by using a plurality of strains known to have drug resistance, and the results show that the antibacterial peptide provided in the present disclosure has broad-spectrum antibacterial activity against drug-resistant strains and more comprehensive antibacterial effects, and allows for wider indications.

The invention discloses an anti-pestivirus broad-spectrum neutralizing monoclonal antibody and application thereof, and belongs to the field of immune globulin. The anti-pestivirus monoclonal antibody or the antigen binding fragment thereof comprises a heavy chain variable region (SEQ ID No.1) and a light chain variable region (SEQ ID No.3). The monoclonal antibody provided by the invention can identify hog cholera virus and all known plague virus members (BVDV1, BVDV2, BDV, HoBiPeV, AydinPeV and OVPV) capable of infecting cattle and sheep at present, has good neutralizing ability, and can be prepared into a diagnostic product for plague virus antigen-antibody detection. The monoclonal antibody and a diagnostic product developed based on the monoclonal antibody can be used for serological diagnosis and immune effect evaluation of pestivirus, pestivirus pathogen detection of experimental serum products and the like, and have wide application prospects in the fields of clinical diagnosis and the like.

The invention relates to bacillus, a biocontrol inoculant prepared from the bacillus and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as QY2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31373, and the preservation time is July 19, 2024. The bacillus QY2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus QY2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention provides a streptomyces, which is named as Streptomyces sp. And is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 65917. The invention also provides a fermentation broth of the streptomyces, and an ethanol extract and a crude extract of the fermentation broth. The novel streptomyces, the crude extract thereof and the like have broad-spectrum antibacterial activity, have a good antagonistic effect on various pathogenic bacteria such as banana long leaf spot germs and banana colletotrichum gloeosporioides, can enable hyphae of the banana colletotrichum gloeosporioides to shrink, deform and break, inhibit spore germination of the banana colletotrichum gloeosporioides, and have a good anti-bacterial effect on the banana colletotrichum gloeosporioides. According to the present invention, with the application of the bacterial strain, the colletotrichum gloeosporioides spores are shrunk and broken, the growth of the colletotrichum gloeosporioides is inhibited, the peroxidase activity, the polyperoxidase activity, the CAT activity and the like in the banana peel can be improved, and the bacterial strain is the potential biological agent for the prevention and control of the colletotrichum gloeosporioides diseases, and has wide development space and good development and application prospects.

The invention belongs to the field of pesticides, and relates to a 2-cyano-3-disubstituted phenyl acrylate compound as well as a preparation method and application thereof in pesticides. Carrying out alcoholysis reaction on the benzonitrile compound a and a hydrogen chloride absolute ethyl alcohol solution to obtain an intermediate b; carrying out condensation reaction on the intermediate b and ethyl cyanoacetate under the action of alkali to obtain an intermediate c; the intermediate c and a substituted compound are subjected to a substitution reaction under the action of alkali and a catalyst, and the 2-cyano-3-disubstituted phenyl acrylate compound I is obtained. The 2-cyano-3-disubstituted phenylacrylate compound provided by the invention has good bactericidal activity, and has relatively high bactericidal activity on pathogenic bacteria of chlorosclerotium, fusarium, acrocyst, colletotrichum, cercospora, verticillium and black beancurd skin; the compound can be used for preparing bactericides in the fields of agriculture, horticulture, industry and the like, and has the advantages of broad spectrum, high efficiency, low toxicity and environmental friendliness.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention discloses an N-cyclobutyl substituted benzamide isoxazoline compound as well as a preparation method and application thereof. The N-cyclobutyl substituted benzamide isoxazoline compound has broad-spectrum and efficient insecticidal activity at a low dosage, and has a remarkable effect on prevention and treatment of diseases and insect pests in agriculture and forestry and pests in the field of sanitation; moreover, the pesticide composition is safer to non-target organisms, can reduce the damage to plants, non-target organisms and human beings due to overhigh pesticide concentration, and is more beneficial to crop protection and environmental safety.

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention belongs to the field of antibacterial peptides, and particularly relates to an antibacterial peptide with a broad-spectrum antibacterial effect, an antibacterial product and application. The antibacterial peptide is named as CAMP1111NC, and is obtained by performing N-terminal acetyl end capping and C-terminal amino end capping on polypeptide shown in SEQ ID NO: 1. The antibacterial peptide has remarkable antibacterial activity on a staphylococcus aureus standard strain ATCC12600, pseudomonas aeruginosa PAO1, klebsiella pneumoniae, an acinetobacter baumannii standard strain ATCC19606, methicillin-resistant staphylococcus aureus ATCC33591, methicillin-resistant and gentamicin-resistant staphylococcus aureus ATCC33592 and acinetobacter baumannii bio-53272 resistant to various antibiotics, and the antibacterial peptide can be used for preparing the antibacterial peptide. The limitation of the traditional antibacterial peptide is effectively overcome.

The invention discloses bacillus subtilis Gxunw-30 and application thereof, and belongs to the technical field of agricultural microorganisms. The bacillus subtilis Gxunw-30 provided by the invention is preserved in Guangdong Microbial Culture Collection Center on October 12, 2024, and the preservation number of the bacillus subtilis Gxunw-30 is GDMCC No: 65245. The bacillus subtilis Gxunw-30 provided by the invention has the capability of inhibiting plant pathogens in a broad spectrum, and can be used for antagonizing a variety of plant pathogens such as helminthosporium triticum, trichosporon pseudoacaceae, hechiense fusarium, fusarium chlamydosporum, cryphonectria parasitica, fusarium pseudograminearum, botryosphaeria dothidea and the like. The strain has the properties of decomposing organophosphorus, producing protease, producing ammonia, producing indole and fixing nitrogen, and has the biological properties of plant growth promotion and the like. The recombinant bacterium also contains a gene for coding an antibacterial substance, and has the capability of synthesizing the antibacterial peptides of iturin, surfactin, fengmycin and bacilysin, and the recombinant bacterium also contains a gene for coding the antibacterial substance and has the capability of synthesizing the antibacterial peptides of iturin, surfactin, fengmycin and bacilysin. The strain has the effects of preventing diseases and promoting growth, provides a good biocontrol resource for prevention and treatment of plant diseases, and has a relatively good application value for research and development of plant growth-promoting preparations and biocontrol microbial agents.

The invention relates to bacillus velezensis, a biocontrol inoculant prepared from the bacillus velezensis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus velezensis provided by the invention is bacillus velezensis and is named as JK1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31375, and the preservation time is July 19, 2024. The bacillus JK1 disclosed by the invention has relatively excellent bacteriostatic action on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus JK1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention relates to the field of super-hydrophobic materials, in particular to a basalt fiber composite material as well as a preparation method and application thereof. According to the composite fabric material provided by the invention, a multistage micro-nano rough surface of the MoS2 nanoflowers and the silver nanoparticles is constructed on the fiber substrate, so that not only is super-hydrophobic self-cleaning realized, but also the anti-icing performance is relatively high, and particularly, rapid heating is realized by combining broadband absorption of the MoS2 nanoflowers and a local surface plasma resonance effect of the silver nanoparticles; and meanwhile, the silver nanoparticles accelerate heat conduction, so that excellent ice melting capability is obtained. The high conductivity of the silver nanoparticles constructs a conductive path, and the layered structure of MoS2 triggers interface polarization, so that the electromagnetic shielding effectiveness of the X wave band is improved. The dual antibacterial mechanism of the MoS2 nanoflowers and the silver nanoparticles also realizes broad-spectrum efficient antibiosis, the preparation method is green and environment-friendly, the cost is low, and the requirements of complex scenes such as wearable electronic equipment, medical protection, polar region protection and national defense can be met.

The invention relates to bacillus amyloliquefaciens, a biocontrol inoculant prepared from the bacillus amyloliquefaciens and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus amyloliquefaciens provided by the invention is bacillus amyloliquefaciens and is named as JK2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CMCC No.31376, and the preservation time is July 19, 2024. The bacillus amyloliquefaciens JK2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus amyloliquefaciens JK2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention relates to the technical field of biological control, in particular to bacillus velezensis JNJK-1, a microbial agent and application of the bacillus velezensis JNJK-1 and the microbial agent in edible fungus disease control, the bacillus velezensis JNJK-1 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CMCC No.31075. The novel bacillus velezensis JNJK-1 is screened out for the first time, and the bacillus velezensis can be applied to biological control of edible mushroom diseases and has a broad-spectrum inhibition effect on pathogenic bacteria of edible mushrooms. Meanwhile, the inventor also finds that the bacillus velezensis JNJK-1, curcumin and sodium alginate are compounded into the microbial agent, so that the inhibition effect on the pathogenic bacteria of the edible fungi can be improved, and the microbial activity can be maintained.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis F0b and an application of the bacillus velezensis F0b. The bacillus velezensis F0b is preserved in the Guangdong Microbial Culture Collection Center on April 26, 2024, the preservation date of the bacillus velezensis F0b is April 26, 2024, and the preservation number of the bacillus velezensis F0b is GDMCC (China General Microbiological Culture Collection Center): 64566. The bacillus velezensis has prevention and / or control effects on pyricularia oryzae, cabbage sclerotinia sclerotiorum, tomato fusarium wilt, banana fusarium wilt, rice leaf spot, peronophythora litchii, soybean carbon rot, colletotrichum capsici and corn leaf curvularia, and can be used for preparing broad-spectrum microbial source bactericides. The fermentation liquor of the bacillus velezensis can generate amylase, carboxymethyl cellulose and protease, and the fermentation liquor of the bacillus velezensis has prevention and / or control effects on vegetable pathogenic fungal diseases, namely cabbage sclerotiniose or pepper anthracnose.

The invention belongs to the field of microorganisms, and relates to a bacillus velezensis BLS-27 and a fungicide and application thereof, the bacillus velezensis BLS-27 is preserved in China General Microbiological Culture Collection Center on October 21, 2024, the preservation number is CGMCC No.32274, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain BLS-27 has an inhibition effect on wild jujube anthracnose pathogenic bacteria, can effectively inhibit various plant disease pathogenic bacteria, shows broad-spectrum antibacterial activity, is environment-friendly and can be widely applied to plant disease control.

The invention provides a novel strain M2108 of a salt-tolerant bacillus, the preservation number of the salt-tolerant bacillus is CGMCC (China General Microbiological Culture Collection Center) No.28283, and the novel strain M2108 of the salt-tolerant bacillus has relatively good salt tolerance and can grow and proliferate in a high-salt environment; the strain can be rapidly and stably colonized in a plant, and is rapid in propagation speed and strong in multiplication capacity in the plant. The new strain M2108 of the salt-tolerant bacillus provided by the invention has a treatment effect and a high control effect on various plant diseases: the control effect on diseases such as tomato root rot, damping-off and damping-off caused by fusarium oxysporum, pythium aphanidermatum and rhizoctonia solani is high, and the average control effect is 91.2% or above. When being used for preventing and treating plant diseases, the salt-tolerant bacillus M2108 is not easy to generate drug resistance, has good drug effect durability, is safe to human and livestock, and does not cause environmental pollution; the salt-tolerant bacillus M2108 microbial agent and the biocontrol agent provided by the invention are simple in preparation method, low in cost and simple and convenient to use. The salt-tolerant bacillus M2108 has a good application prospect in the prevention and control of plant diseases, and lays a foundation for the development of efficient and broad-spectrum biocontrol preparations.

The invention discloses a milk-derived broad-spectrum antibacterial peptide and application thereof, and relates to the technical field of biology. The amino acid sequence of the antibacterial peptide is as shown in any one of SEQ ID NO.2-6. Five novel antibacterial peptides are obtained by carrying out symmetrical structure design and amino acid mutation on the basis of a template peptide, and tests find that the five novel antibacterial peptides have broad-spectrum antibacterial activity which is obviously higher than that of the template peptide, and basically have no cytotoxicity. Wherein the geometric mean (GM) of the minimum inhibitory concentration can reach 3.60, and the therapeutic index (TI) can reach 71.11. The antibacterial peptide prepared by the invention still keeps good antibacterial activity in physiological salt concentration and protease environment, has certain serum stability, and has higher application value; in addition, the antibacterial peptide has a good inhibition effect on the formation of a bacterial biofilm, and has a synergistic effect with traditional antibiotics.

The invention relates to a novel antibacterial peptide sequence intelligent generation method and system and computer equipment, and the method comprises the steps: inputting a first antibacterial peptide sequence data set containing multi-sequence alignment into a pre-trained generator, and outputting a novel first candidate antibacterial peptide sequence set according to a preset condition; inputting the first candidate antibacterial peptide sequence set into a pre-trained discriminator to discriminate a second candidate antibacterial peptide sequence set belonging to the antibacterial peptide sequence; inputting the second candidate antibacterial peptide sequence set into a pre-trained predictor to predict the antibacterial activity of each second candidate antibacterial peptide sequence in the second candidate antibacterial peptide sequence set; and based on the antibacterial activity of each second candidate antibacterial peptide sequence, selecting at least one optimal target antibacterial peptide sequence from each second candidate antibacterial peptide sequence set. According to the application, the generator can be used for capturing sequence diversity and generating a wider-spectrum antibacterial peptide sequence, and through cascade optimization of the discriminator and the predictor, the generated antibacterial peptide sequence has higher antibacterial activity.