1215 results about "Pathogenic microorganism" patented technology

The invention discloses a capture probe group for detecting pathogenic microorganisms and application of the capture probe group. The nucleic acid sequence of the capture probe group comprises a sequence as shown in SEQ ID NO. 1 to SEQ ID NO. 101. The capture probe group can cover and detect 119 types of bacteria, 32 types of fungi, 41 types of DNA viruses and 71 types of RNA viruses and covers common infectious disease pathogens, the kit containing the capture probe can cover and detect 88 types of drug-resistant and virulence genes, the detection performance of pathogenic microorganisms is greatly improved, and the sensitivity and specificity are improved.

The invention discloses a primer probe combination and a kit for detecting genital tract pathogenic microorganisms and application. The primer probe combination comprises primer probe sequences as shown in SEQ ID NO.1-SEQ ID NO.96, the genital tract pathogens comprise 27 pathogens capable of causing genital tract diseases, such as ureaplasma parvum, ureaplasma urealyticum, mycoplasma hominis, mycoplasma genitalium, candida albicans, candida glabrata, candida tropicalis, candida auricula, candida krusei and candida duberlin, as well as lactobacillus crispatus, lactobacillus jensenii and lactobacillus gasseri; the lactobacillus lactis comprises four common vaginal lactobacillus lactis, and also comprises a human-derived interior label. On the basis of a fluorescent quantitative PCR technology, the kit can be used for simultaneously detecting 27 pathogens capable of causing genital tract diseases and 4 common lactobacillus vaginalis in an in-vitro sample, not only can cover main pathogens of genital tract infection, but also meets the evaluation on the ecological environment of the vagina, the detection time is shortened to about 40 minutes, and meanwhile, the kit has relatively good specificity and sensitivity.

The invention discloses a plant lactobacillus with a bacteriostatic effect and application of the plant lactobacillus. The lactobacillus plantarum is named as lactobacillus plantarum C07, the preservation number is CCTCC NO: M20242086, the preservation unit is China Center for Type Culture Collection, the preservation time is September 26, 2024, and the preservation address is Wuhan University, Wuhan University, No. 299, eight road, Wuchang District, Wuhan City, Hubei Province. The strain can produce lactic acid and indole-3-lactic acid at high yield, has a very strong antibacterial effect on multiple putrefying and pathogenic microorganisms such as staphylococcus aureus, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella and mould, and is a broad-spectrum antibacterial microorganism.

The invention relates to the technical field of environmental monitoring, in particular to a water body pathogenic microorganism source analysis method and system, and the method comprises the following steps: based on water body sample collection information, identifying a plurality of moisture sources of a target water body through the sampling position of the water body, calculating the contribution degree of a plurality of source water bodies to a sampling point, and generating a water body position analysis result. According to the method, the prediction accuracy of the pollution risk area is improved by identifying the multiple sources of the water body, calculating the contribution degrees of the multiple water body sources to the sampling points and combining real-time meteorological and topographic data to simulate the diffusion path of pollution, and the prediction capability of the pollution source type is improved by evaluating the microbial population distribution mode in the water body sample. Data support is provided for pollution source position identification, multiple source positions of pathogenic microorganisms are identified through comparison with microorganism samples of multiple pollution sources, a scientific basis is provided for taking effective water quality protection and disease prevention measures, and the quality and efficiency of environment management are improved.

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention belongs to the technical field of pathogenic microorganism detection, and particularly relates to a sequence combination for detecting cochliobolus miyabeanus based on RPA-CRISPR / Cas12b and application of the sequence combination. The CRISPR / Cas system contained in the composition comprises Cas12b and sgRNA, and the CRISPR / Cas system can be cooperatively used with a specific reaction system. The rapid detection method comprises the following steps: sample pretreatment: treating a sample to extract nucleic acid; performing amplification reaction: performing RPA amplification reaction on the extracted nucleic acid by using the composition, and applying a nucleic acid amplification product to CRISPR / Cas system detection; result interpretation: reading and judging a detection result through a quality control line and a detection line. The composition and the kit provided by the invention are specially used for detecting the cochliobolus miyabeanus. The method is simple and convenient to operate, rapid in detection, short in time consumption, low in cost and suitable for on-site rapid detection.

The invention discloses a split type micro-fluidic chip and a working method thereof. In the chip, a reagent storage module is connected with a reaction module through a connection module, and the reaction module is slidably arranged in a chute of the connection module; a plurality of solution containing cavities are formed in the reagent storage module, a sealing film is attached to the top of the reaction module, and a plurality of solution overflowing cavities are formed in the reaction module; during work, the reaction module and the reagent storage module abut against each other, and when the solution containing cavity is communicated with the atmosphere, directional flowing and releasing of a solution in the solution containing cavity in the micro-fluidic chip to the solution overflowing cavity are achieved through negative pressure pumping. Sample sampling, cleaning, nucleic acid adsorption, elution and reaction in the micro-fluidic chip can be achieved, and instant detection of pathogenic microorganisms is achieved.

The invention relates to the technical field of molecular biological detection, and discloses a composition for detecting pathogenic microorganisms, a kit and application of the composition and the kit. The composition provided by the invention can simultaneously comprise specific primers aiming at a plurality of different pathogenic microorganisms and has excellent specificity and detection sensitivity to the specific pathogenic microorganisms, and different primers do not generate dimerization, so that the different pathogenic microorganisms can be simultaneously, rapidly and accurately detected.

The invention provides an artificial grouper breeding method suitable for high latitude areas, and relates to the technical field of grouper culture, the method specifically comprises the following steps: S1, transportation and treatment of grouper fertilized eggs; s2, breeding grouper seedlings in the high-latitude region; s3, preparing and transporting the juvenile fish from the high-latitude area to the low-latitude area; and S4, carrying the juvenile fish back to the low-latitude region, and carrying out adaptive culture. According to the technical scheme, after the fertilized eggs of the groupers in the low-latitude area are conveyed to the high-latitude area for fry breeding, the groupers are bred in the low-latitude area, negative effects of pathogenic microorganisms and water bodies on the groupers under the natural habitat are effectively avoided, and the occurrence of diseases of the grouper fries is effectively prevented; and the quality and yield of grouper culture are improved.

The invention discloses a liquid storage type micro-fluidic chip and a working method thereof. The chip comprises a chip body, a sealing film and gum, the sealing film and the back adhesive are respectively bonded on the front surface and the back surface of the chip body; a plurality of solution storage cavities are formed in the chip body, outflow runners at the bottoms of the solution storage cavities are connected with corresponding first runners, sealing partition pieces are arranged on the first runners, the outflow runners and the corresponding first runners are blocked through the sealing partition pieces, and the first runners are communicated with second runners through corresponding gum runners. The whole experiment process does not need to be externally connected with a reagent bottle, the purposes of instant detection, preparation and sample separation of the micro-fluidic chip can be achieved, the detection time is greatly shortened, and the experiment steps are simplified. Waste liquid generated in the experiment process flows into the waste liquid cavity, and cross contamination of a laboratory is avoided. The chip provided by the invention can be used for identifying the species of pathogenic microorganisms.

The present invention discloses a method and system for designing primers for sequencing targeted pathogenic microorganisms, the method comprising the following steps: step 1) obtaining genome data of pathogenic microorganisms and establishing a pathogenic microorganism genome database; step 2) obtaining the conserved regions of pathogenic microorganisms according to the pathogenic microorganism genome database; step 3) designing multiple primers according to the conserved regions in step 2); step 4) evaluating the specificity and inclusiveness of the multiple primers designed in step 3), and selecting multiple primers with specificity and inclusiveness of ≥90%. The primer set obtained in the present invention can detect multiple strains of pathogens at the same time, and the primer detection results are more accurate than those obtained in the prior art.

The invention discloses a construction method and application of a pathogenic microorganism genome database. The method comprises the steps of genome acquisition, genome screening, target region grabbing, strain verification, target region sequence clustering, redundancy elimination and database merging. The construction method of the pathogenic microorganism genome database is suitable for targeted high-throughput sequencing, and the corresponding amplification relationship between the primer and the corresponding microorganism genome sequence is obtained through the operations of screening, filtering, simulation amplification and the like on pathogenic microorganism genome data; the risk of wrong identification of pathogenic species can be reduced by constraining the corresponding relationship.

The invention discloses a multiplex fluorescent PCR (polymerase chain reaction) kit and a detection method for multiple pathogenic microorganisms and drug-resistant genes. The kit comprises a primer and a fluorescent probe which are used for detecting target pathogenic microorganisms and drug-resistant genes, a PCR reaction premix solution, a positive quality control product and a negative quality control product. A plurality of fluorescent channels are used for respectively marking corresponding detection targets, and the amplification conditions of different targets are monitored by monitoring the change conditions of fluorescent signals in different fluorescent channels, so that the detection of 96 pathogenic microorganisms and drug-resistant genes is realized. The multiplex fluorescent PCR kit disclosed by the invention can be used for simultaneously detecting various pathogenic microorganisms and drug-resistant genes, and is high in accuracy, strong in specificity, high in sensitivity, rapid and reliable in result.

The invention relates to digestive tract mucosa protective agent powder as well as a preparation method and application thereof, and the digestive tract mucosa protective agent powder contains gel and a cross-linking agent, the gel comprises any one or a combination of at least two of cellulose, modified cellulose, alginate, modified alginate, sodium polyglutamate, modified sodium polyglutamate, starch, modified starch, sodium hyaluronate, modified sodium hyaluronate, pectin or modified pectin, and the cross-linking agent comprises an ionic cross-linking agent or a nonionic cross-linking agent. The powder protective agent provided by the invention is convenient to produce and simple to use, can be adhered to the surface of a mucous membrane wound surface only through simple spraying operation, forms a hydrogel protective layer under the action of gastric juice, intestinal juice or other liquid on the surface of the wound surface, plays a role of a physical barrier, and has a good application prospect. The continuous erosion of digestible liquid or pathogenic microorganisms on the surface of the wound surface to the wound surface is effectively blocked.

The invention relates to the technical field of rapid detection of pathogenic microorganisms, in particular to a primer probe combination for detecting salmonella pullorum based on fluorescent RT-RAA, a kit and a use method. The primer for detecting salmonella pullorum based on the fluorescence method RT-RAA is as shown in SEQ ID NO. 1 and SEQ ID NO. 4. According to the kit developed by combining the primer with a probe, salmonella pullorum in a sample can be rapidly and efficiently detected by using an RT-RAA fluorescence method, and the kit is high in sensitivity and good in repeatability and has a good application prospect in development of related rapid detection.

The invention relates to a method for carrying out pathogenic microorganism identification and gene variation state joint detection on a pulmonary alveolar lavage fluid sample based on high-throughput sequencing. The method comprises the following steps: extracting nucleic acid from the pulmonary alveolar lavage fluid sample; constructing a metagenome capture library, and constructing a lung cancer polygene library; carrying out final library pooling; performing high-throughput sequencing; and analyzing data. Compared with the prior art, the scheme provided by the invention is based on a technical path of'macro 'captured by metagenome and'target' of targeted high-throughput sequencing; after the constructed final library is subjected to high-throughput sequencing and data analysis, index output (pathogenic microorganism identification, host chromosome copy number variation, gene mutation, gene fusion, gene copy number variation and methylation state) of multiple biomarkers can be met in one-time detection.

The invention belongs to the technical field of biological control, and particularly relates to the technical field of crop stem rot control. The invention provides a bacillus velezensis Gc8, and the preservation number of the bacillus velezensis Gc8 is CGMCC (China General Microbiological Culture Collection Center) No. 28991. The bacillus velezensis Gc8 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 14, 2023, the preservation number of the bacillus velezensis Gc8 is CGMCC No.28991, the center is called CGMCC for short, and the address of the center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67, kitasapora fluorescens pf27, kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27. The compound microbial agent can inhibit spore germination of pathogenic microorganisms, and has the effect of promoting growth of corn while resisting diseases.

The invention discloses an identification and analysis method for unknown pathogenic microorganisms, which comprises the following steps: filtering out genome sequences with low integrity, pollution and tag errors, and establishing a high-quality virus identification database; constructing a virus host prediction model through a machine learning algorithm; unknown pathogenic microorganisms are identified and analyzed, potential hosts or pathogenicity of the unknown microorganisms are identified, and whether the unknown microorganisms are unknown pathogenic viruses or bacteria or not is further judged. On the basis of metagenome data analysis, potential unknown pathogenic microorganisms in samples of human bodies, environments and the like can be identified more accurately.

The invention belongs to the technical field of biomedical treatment, and discloses a preparation method of immunomodulatory recombinant macrophage vesicles, and the preparation method specifically comprises the following steps: step 1, clinical sequencing of gingival tumor tissues and pathogenic microbiota data analysis; hybridizing and fusing a macrophage membrane infected by the porphyromonas gingivalis and an exosome membrane; and 3, constructing a piezoelectric driven ROS (reactive oxygen species) to generate the immunoregulation vesicles. The invention provides an innovative nano vesicle technology, and the technology shows double advantages in tumor treatment: firstly, accurate targeting and efficient killing of key porphyromonas gingivalis in a tumor environment are realized by constructing recombinant macrophage vesicles, and meanwhile, the stability of normal flora is kept, and tumor deterioration is prevented; and secondly, the anti-tumor immune response is obviously activated by utilizing the immunoregulation capability of the bacteria-induced macrophages and exosome vesicles thereof, including converting immunosuppressed M2 type macrophages into anti-tumor M1 type macrophages, so that the growth of solid tumors is effectively inhibited.

The invention relates to the technical field of biomedicine, in particular to a marker for identifying pathogenic microorganism types based on host reaction, which comprises a combination of coding RNA (Ribonucleic Acid) or proteins coded by the combination of the coding RNA: SLC37A3 and UBALD2. The biomarker can be used for diagnosing the types of pathogenic microorganisms and distinguishing bacterial or virus infection. Moreover, diagnosis can be carried out through the marker in the early stage of infection, and the marker has the advantages of being short in consumed time, high in specificity, high in sensitivity and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to a pleiotropic protein MEL3 and application thereof. The amino acid sequence of the pleiotropic protein MEL3 or the homologous protein of the pleiotropic protein MEL3 provided by the invention has the following general formula: X1GAX2LX3VLX4GLX5ALISWEKRKRX6, x1 is M or L; the X2 is one of V, SI and AI; x3 is one of K, T, KI and KR; x4 is one of KK, TT, SS and VK; x5 is one of P, K, A and T; and X6 is QQ or is free of amino acid. The pleiotropic protein MEL3 or the homologous protein thereof provided by the invention has broad-spectrum antibacterial activity, can achieve anti-inflammatory and antiviral effects at the same time, and provides a new thought for preparing anti-pathogenic microorganism or anti-inflammatory products.

The invention provides a culture method for improving immunity of fishes. The culture method comprises the following steps: S1, selection of culture points; S2, specification and layout of net cages; S3, selection and throwing of fish fries; according to the fry disinfection method disclosed by the invention, the fry are soaked and disinfected by using a compound disinfectant prepared from povidone iodine, polysorbate, citric acid and vitamin C in a specific proportion, so that pathogenic microorganisms on the surfaces and gill parts of fish bodies are effectively killed. According to the breeding management, the immunity of the fish body is comprehensively enhanced by feeding the fish with an immune conditioner composed of campesterol palmitate, immunomodulatory peptide, beta-glucan and astaxanthin in a specific proportion. According to the bottom mud management, the content of harmful substances is reduced by putting a bottom mud modifier composed of sodium molybdate, nitrifying bacteria, photosynthetic bacteria, tetrandrine and sodium alginate in a specific proportion, growth of pathogenic bacteria is inhibited, nutrient substance circulation is promoted, the water quality environment is optimized, and healthy growth of fry is promoted.

The invention provides a probe set and method for detecting respiratory tract infection pathogenic microorganisms and application of the probe set and method. The probe group is high in specificity and sensitivity, capable of accurately capturing target species in a pathogen spectrum and efficiently enriching the target species, meanwhile, few library fragments of non-target species are introduced, pathogenic microorganisms are convenient to identify, the detection sensitivity is high, the accuracy is good, the time cost is saved, the coverage degree is wide, and the application prospect is wide. Various bacteria, fungi, viruses and other pathogenic microorganisms related to respiratory tract infection can be covered.

The invention discloses an unknown pathogenic microorganism identification and analysis system and method based on nanopore sequencing. Comprising the steps of microorganism database construction and updating, microorganism information standardization labeling, nanopore sequencing analysis, sequencing data quality control, host sequencing read segment removal, microorganism database comparison annotation, congeneric sequencing read segment assembly and microorganism database comparison calculation. For each representative sequencing read segment, calculating and comparing the representative sequencing read segment with any genome sequence in the microorganism database, when a first calculation and comparison condition is met, outputting that the representative sequencing read segment is consistent with the species to which the genome sequence meeting the first calculation and comparison condition belongs, and when a second calculation and comparison condition is met, outputting that the representative sequencing read segment is consistent with the species to which the genome sequence meeting the first calculation and comparison condition belongs. And outputting that the representative sequencing read and the species to which the sequence meeting the second calculation comparison condition belongs are in a homologous relationship, and when the representative sequencing read and each sequence in the microorganism database do not meet the two conditions, outputting that the representative sequencing read is not compared in the microorganism database.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention discloses an osteomyelitis wound microenvironment detection system, and relates to the technical field of osteomyelitis wound microenvironment detection.The osteomyelitis wound microenvironment detection system comprises a leg placing support, a detection support is arranged at the top of the leg placing support and comprises a positioning support and a movable support, and a sensor mechanism is arranged at the end, close to a buckle, of the movable support; the height and the position of the detection support are adjusted, so that the position of a wound surface suitable for a patient can be conveniently selected according to the height and the weight of the patient, and the patient can conveniently take the wound surface of the patient. The sensor array, the electrode system, the signal converter and the data processing module are integrated in the portable equipment, so that relevant indexes such as pH value, humidity and pathogenic microorganisms around a bone infection wound surface can be monitored in real time, various environmental parameters are effectively and comprehensively processed and analyzed, more comprehensive microenvironment information is provided, and the application prospect is wide. And bone infection wounds can be accurately monitored for a long time.

The invention relates to methods of screening biological samples from a subject for the presence of one or more microorganisms, such as the presence in the subject of one or more pathogenic or potentially pathogenic microorganisms, and diagnostic kits and devices suitable for carrying out said methods. More particularly, the present invention relates to sensitive and specific methods for determining the presence of gram-negative bacteria or analytes therefrom in a sample from a subject using immunoassays, and devices and kits for carrying out such methods.

The invention relates to the technical field of soil improvement, and discloses a soil improvement repairing agent and a preparation method thereof. The preparation method comprises the following steps: adding biochar, modified eugenol, modified benzothiazolyl pyridine, bacillus licheniformis and diatomite into a mixer, mixing and stirring, extruding and granulating in a granulator, and drying to obtain the soil improvement repairing agent. Eugenol can destroy a membrane structure and denature protein, so that a bacteriostatic effect is achieved, and a healthy soil microenvironment is provided for crops; the quaternary ammonium salt can destroy the membrane structure of pathogenic microorganisms, plays a role in killing the microorganisms, can improve the antibacterial effect of the remediation agent, and prevents and treats spread diseases of soil; nitrogen atoms and sulfur atoms in benzothiazolyl pyridine molecules can coordinate with free heavy metal ions in the soil, the heavy metal adsorption effect of the remediation agent is improved, the production value of the soil is gradually restored, meanwhile, boric acid capable of being absorbed by plants can be released through hydrolysis of pinacol ester, and the fertility of the soil is improved.

The invention discloses a respiratory infectious disease multi-pathogen nucleic acid detection kit based on a fluorescent PCR detection technology and application of the respiratory infectious disease multi-pathogen nucleic acid detection kit. The invention provides two detection kits aiming at 20 and 6 respiratory tract detection, the two kits can specifically detect respiratory tract pathogens, the detection sensitivity is high, the whole detection system is short in time consumption and simple and convenient in use process, and detection results of 20 pathogenic microorganisms can be reported within 40 min at the shortest; the whole set of detection kit and the detection method are high in sensitivity and good in specificity; the normal-temperature storage and transportation of the reagent can be realized.

The invention relates to the technical field of sewage treatment, in particular to a rural sewage treatment process and system.The rural sewage treatment process comprises the steps that S1, sewage pretreatment is conducted through a raw water pool to remove large floating objects and suspended solids in sewage, and the pretreated sewage is conveyed to a biochemical reaction pool; s2, monitoring water quality parameters in real time through water quality detection equipment in the biochemical reaction tank; s3, inputting the water quality parameters into a pre-trained neural network model to output operation parameters of the biochemical reaction tank; s4, performing biochemical treatment and advanced treatment on the sewage in the biochemical reaction tank according to the operation parameters, the biochemical treatment including removal of organic matters, nitrogen and phosphorus removal and reduction of the number of pathogenic microorganisms, and the advanced treatment including further removal of suspended solids, removal of refractory organic matters and disinfection and sterilization. And the operation parameters are flexibly obtained through the neural network model, so that the sewage treatment flexibility is improved, and the resource waste is reduced.