854 results about "Pathogenic microorganism" patented technology

The invention discloses a plant lactobacillus with a bacteriostatic effect and application of the plant lactobacillus. The lactobacillus plantarum is named as lactobacillus plantarum C07, the preservation number is CCTCC NO: M20242086, the preservation unit is China Center for Type Culture Collection, the preservation time is September 26, 2024, and the preservation address is Wuhan University, Wuhan University, No. 299, eight road, Wuchang District, Wuhan City, Hubei Province. The strain can produce lactic acid and indole-3-lactic acid at high yield, has a very strong antibacterial effect on multiple putrefying and pathogenic microorganisms such as staphylococcus aureus, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella and mould, and is a broad-spectrum antibacterial microorganism.

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention belongs to the technical field of pathogenic microorganism detection, and particularly relates to a sequence combination for detecting cochliobolus miyabeanus based on RPA-CRISPR / Cas12b and application of the sequence combination. The CRISPR / Cas system contained in the composition comprises Cas12b and sgRNA, and the CRISPR / Cas system can be cooperatively used with a specific reaction system. The rapid detection method comprises the following steps: sample pretreatment: treating a sample to extract nucleic acid; performing amplification reaction: performing RPA amplification reaction on the extracted nucleic acid by using the composition, and applying a nucleic acid amplification product to CRISPR / Cas system detection; result interpretation: reading and judging a detection result through a quality control line and a detection line. The composition and the kit provided by the invention are specially used for detecting the cochliobolus miyabeanus. The method is simple and convenient to operate, rapid in detection, short in time consumption, low in cost and suitable for on-site rapid detection.

The invention discloses a construction method and application of a pathogenic microorganism genome database. The method comprises the steps of genome acquisition, genome screening, target region grabbing, strain verification, target region sequence clustering, redundancy elimination and database merging. The construction method of the pathogenic microorganism genome database is suitable for targeted high-throughput sequencing, and the corresponding amplification relationship between the primer and the corresponding microorganism genome sequence is obtained through the operations of screening, filtering, simulation amplification and the like on pathogenic microorganism genome data; the risk of wrong identification of pathogenic species can be reduced by constraining the corresponding relationship.

The invention relates to a method for carrying out pathogenic microorganism identification and gene variation state joint detection on a pulmonary alveolar lavage fluid sample based on high-throughput sequencing. The method comprises the following steps: extracting nucleic acid from the pulmonary alveolar lavage fluid sample; constructing a metagenome capture library, and constructing a lung cancer polygene library; carrying out final library pooling; performing high-throughput sequencing; and analyzing data. Compared with the prior art, the scheme provided by the invention is based on a technical path of'macro 'captured by metagenome and'target' of targeted high-throughput sequencing; after the constructed final library is subjected to high-throughput sequencing and data analysis, index output (pathogenic microorganism identification, host chromosome copy number variation, gene mutation, gene fusion, gene copy number variation and methylation state) of multiple biomarkers can be met in one-time detection.

The invention belongs to the technical field of biological control, and particularly relates to the technical field of crop stem rot control. The invention provides a bacillus velezensis Gc8, and the preservation number of the bacillus velezensis Gc8 is CGMCC (China General Microbiological Culture Collection Center) No. 28991. The bacillus velezensis Gc8 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 14, 2023, the preservation number of the bacillus velezensis Gc8 is CGMCC No.28991, the center is called CGMCC for short, and the address of the center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67, kitasapora fluorescens pf27, kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27. The compound microbial agent can inhibit spore germination of pathogenic microorganisms, and has the effect of promoting growth of corn while resisting diseases.

The invention discloses an identification and analysis method for unknown pathogenic microorganisms, which comprises the following steps: filtering out genome sequences with low integrity, pollution and tag errors, and establishing a high-quality virus identification database; constructing a virus host prediction model through a machine learning algorithm; unknown pathogenic microorganisms are identified and analyzed, potential hosts or pathogenicity of the unknown microorganisms are identified, and whether the unknown microorganisms are unknown pathogenic viruses or bacteria or not is further judged. On the basis of metagenome data analysis, potential unknown pathogenic microorganisms in samples of human bodies, environments and the like can be identified more accurately.

The invention relates to the technical field of biomedicine, in particular to a marker for identifying pathogenic microorganism types based on host reaction, which comprises a combination of coding RNA (Ribonucleic Acid) or proteins coded by the combination of the coding RNA: SLC37A3 and UBALD2. The biomarker can be used for diagnosing the types of pathogenic microorganisms and distinguishing bacterial or virus infection. Moreover, diagnosis can be carried out through the marker in the early stage of infection, and the marker has the advantages of being short in consumed time, high in specificity, high in sensitivity and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to a pleiotropic protein MEL3 and application thereof. The amino acid sequence of the pleiotropic protein MEL3 or the homologous protein of the pleiotropic protein MEL3 provided by the invention has the following general formula: X1GAX2LX3VLX4GLX5ALISWEKRKRX6, x1 is M or L; the X2 is one of V, SI and AI; x3 is one of K, T, KI and KR; x4 is one of KK, TT, SS and VK; x5 is one of P, K, A and T; and X6 is QQ or is free of amino acid. The pleiotropic protein MEL3 or the homologous protein thereof provided by the invention has broad-spectrum antibacterial activity, can achieve anti-inflammatory and antiviral effects at the same time, and provides a new thought for preparing anti-pathogenic microorganism or anti-inflammatory products.

The invention provides a probe set and method for detecting respiratory tract infection pathogenic microorganisms and application of the probe set and method. The probe group is high in specificity and sensitivity, capable of accurately capturing target species in a pathogen spectrum and efficiently enriching the target species, meanwhile, few library fragments of non-target species are introduced, pathogenic microorganisms are convenient to identify, the detection sensitivity is high, the accuracy is good, the time cost is saved, the coverage degree is wide, and the application prospect is wide. Various bacteria, fungi, viruses and other pathogenic microorganisms related to respiratory tract infection can be covered.

The invention discloses an unknown pathogenic microorganism identification and analysis system and method based on nanopore sequencing. Comprising the steps of microorganism database construction and updating, microorganism information standardization labeling, nanopore sequencing analysis, sequencing data quality control, host sequencing read segment removal, microorganism database comparison annotation, congeneric sequencing read segment assembly and microorganism database comparison calculation. For each representative sequencing read segment, calculating and comparing the representative sequencing read segment with any genome sequence in the microorganism database, when a first calculation and comparison condition is met, outputting that the representative sequencing read segment is consistent with the species to which the genome sequence meeting the first calculation and comparison condition belongs, and when a second calculation and comparison condition is met, outputting that the representative sequencing read segment is consistent with the species to which the genome sequence meeting the first calculation and comparison condition belongs. And outputting that the representative sequencing read and the species to which the sequence meeting the second calculation comparison condition belongs are in a homologous relationship, and when the representative sequencing read and each sequence in the microorganism database do not meet the two conditions, outputting that the representative sequencing read is not compared in the microorganism database.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention relates to the technical field of soil improvement, and discloses a soil improvement repairing agent and a preparation method thereof. The preparation method comprises the following steps: adding biochar, modified eugenol, modified benzothiazolyl pyridine, bacillus licheniformis and diatomite into a mixer, mixing and stirring, extruding and granulating in a granulator, and drying to obtain the soil improvement repairing agent. Eugenol can destroy a membrane structure and denature protein, so that a bacteriostatic effect is achieved, and a healthy soil microenvironment is provided for crops; the quaternary ammonium salt can destroy the membrane structure of pathogenic microorganisms, plays a role in killing the microorganisms, can improve the antibacterial effect of the remediation agent, and prevents and treats spread diseases of soil; nitrogen atoms and sulfur atoms in benzothiazolyl pyridine molecules can coordinate with free heavy metal ions in the soil, the heavy metal adsorption effect of the remediation agent is improved, the production value of the soil is gradually restored, meanwhile, boric acid capable of being absorbed by plants can be released through hydrolysis of pinacol ester, and the fertility of the soil is improved.

The invention discloses a respiratory infectious disease multi-pathogen nucleic acid detection kit based on a fluorescent PCR detection technology and application of the respiratory infectious disease multi-pathogen nucleic acid detection kit. The invention provides two detection kits aiming at 20 and 6 respiratory tract detection, the two kits can specifically detect respiratory tract pathogens, the detection sensitivity is high, the whole detection system is short in time consumption and simple and convenient in use process, and detection results of 20 pathogenic microorganisms can be reported within 40 min at the shortest; the whole set of detection kit and the detection method are high in sensitivity and good in specificity; the normal-temperature storage and transportation of the reagent can be realized.

The invention belongs to the technical field of food preservation, and particularly relates to a chitosan / polyvinyl alcohol film loaded beta-acid / beta-cyclodextrin inclusion compound as well as a preparation method and application thereof. The method comprises the following steps: adding beta-acid / beta-cyclodextrin into distilled water, grinding and centrifuging, and freeze-drying the obtained supernatant to obtain the beta-acid / beta-cyclodextrin inclusion compound. The preparation method comprises the following steps: mixing a PVA solution with a chitosan solution, adding glycerol to obtain a mixed solution, dissolving the beta-acid / beta-cyclodextrin inclusion compound in water, adding the dissolved beta-acid / beta-cyclodextrin inclusion compound into the mixed solution, stirring, standing and drying to obtain the film. The prepared film has good physical properties and stability, has good oxidation resistance and antibacterial activity, can inhibit adhesion of pathogenic microorganisms such as staphylococcus aureus on the surfaces of perishable fruits, and prolongs the shelf life of the perishable fruits.

The invention discloses a primer combination for identifying six genital tract pathogens and detecting drug resistance genes, a kit and application, the primer combination comprises a primer combination for identifying chlamydia trachomatis, mycoplasma genitalium, ureaplasma urealyticum, mycoplasma hominis, neisseria gonorrhoeae and trichomonas vaginalis and detecting the drug resistance genes of the six genital tract pathogens, and nucleotide sequences are shown as SEQ ID NO.1-SEQ ID NO.50. Microorganisms and related drug-resistant genes in a detection range are identified by utilizing a multiple PCR targeted three-generation sequencing method, the specificity is good, the sensitivity is high, the detection technology has important clinical significance when being applied to identification of genital tract infection pathogens, and compared with a traditional detection method, the detection flux, the coverage range and the sensitivity are higher, and detection is stable; compared with pathogenic microorganism metagenome (mNGS) detection, the method has the advantages that the detection cost is lower, and the detection sensitivity on drug-resistant genes and drug-resistant gene mutation is higher.

The invention relates to the technical field of solar heat pumps, in particular to an air source heat pump control method and system based on deep learning. Comprising the following steps that S1, the liquid working medium temperature of a solar heat storage tank is obtained through a temperature sensor, the solar radiation intensity is obtained through a sensor, and auxiliary management of the air source heat pump is judged according to the liquid working medium temperature of the solar heat storage tank and the solar radiation intensity; and S2, relevant data of the solar heat storage tank are obtained, an adjustment value is obtained by using an adjustment processing formula according to the relevant data, and the liquid working medium in the solar heat storage tank is heated by using an air source heat pump according to the adjustment value. By combining the advantages of the solar photo-thermal system and the air source heat pump, stable heat supply under various environmental conditions is guaranteed, the liquid working medium in the heat storage tank is heated according to a parameter adjustment formula, and breeding of pathogenic microorganisms is eradicated.

The invention discloses an intelligent cross infection prevention disinfection closestool, and relates to the field of disinfection closestools. The toilet seat is arranged on the base; the water tank is arranged on the base; the electrolysis module is arranged in the water tank and used for electrolyzing tap water to obtain disinfectant fluid; the toilet seat ring is sleeved with the seat ring sleeve; the automatic sleeve replacing device is arranged on the base and used for pulling one end of the seat sleeve to move in the circumferential direction of the toilet seat; the wetting device is arranged on the base and used for spraying disinfectant fluid to the seat ring sleeve; and the control module is arranged on the base and is used for controlling the electrolysis module, the automatic sleeve changing device and the wetting device to start / stop. According to the application, the situation that a disinfectant needs to be frequently added externally is avoided, the effect of automatically disinfecting the environment in the base and the toilet seat at the same time is achieved, the risks of contact propagation and aerosol propagation caused by the toilet are integrally solved, the probability of pathogenic microorganism residues and diffusion is reduced, and cross infection is avoided.

The invention relates to the technical field of biological detection, and particularly discloses a farm animal excrement pathogenic microorganism automatic sampling detection method, which comprises the following steps: integrating and analyzing farm three-dimensional geographic information, historical epidemic situation data and real-time environmental parameters by using a deep learning algorithm, and generating a pathogenic microorganism risk thermodynamic diagram; driving an autonomous navigation vehicle equipped with a multispectral sensor, performing dynamic path planning according to the risk thermodynamic diagram, and preferentially collecting an excrement sample in a high-risk area; through the risk thermodynamic diagram generated by a deep learning algorithm, accurate positioning of a sampling area is realized, blind sampling is reduced, the sampling process is autonomously completed in combination with an autonomous navigation vehicle, flexible operation can be performed in a complex environment, efficient execution of a sampling task is ensured, and meanwhile, due to the use of a bionic micro-fluidic chip, the sampling efficiency is improved. Not only is high-efficiency separation of sticky substances and microorganisms realized, but also the recovery rate of pathogenic microorganisms is greatly improved.

The invention provides a design method of a specific gene probe for detecting pathogenic microorganisms, and belongs to the technical field of microorganism detection. Comprising the following steps: acquiring reference genome data of target pathogenic bacteria, reference genome data of all species belonging to the same genus as the target pathogenic bacteria and genome data of all strains under the target pathogenic bacteria species; comparing the reference genome data of the target pathogenic bacteria with the reference genome data of all target pathogenic bacteria congeneric species by using MUMmer to obtain all fragments in the reference genome of the target pathogenic bacteria, wherein the base number of the fragments is greater than or equal to 20 when the fragments are continuously compared with the reference genomes of other congeneric microorganisms, and the base number of the fragments is greater than or equal to 20 when the fragments are continuously compared with the reference genomes of other congeneric microorganisms; recording starting base sites and ending base sites of all fragments; breaking a reference genome of the target pathogenic bacteria into a 50nt k-mer set, and filtering out non-specific fragments in the 50nt k-mer set to obtain an initial candidate probe set; filtering the initial candidate probe set to obtain a candidate probe set 3; and filtering the candidate probe set 3 to obtain a specific probe set. According to the design method, the specific probe is directly mined in the genomic data of the pathogenic microorganisms, large-scale probe design work can be competent, the designed probe has excellent resolution, the accuracy of pathogenic microorganism detection can be improved, and accurate detection of the pathogenic microorganisms is achieved.

The present invention relates to a fungicidal composition comprising components (A) and (B), wherein components (A) and (B) are as defined in claim 1; and the use of the composition in agriculture or horticulture for controlling or preventing infestation of plants by phytopathogenic microorganisms, preferably fungi.

The invention discloses a targeted sequencing method for various pathogenic microorganisms. The targeted sequencing method specifically comprises the following steps: collecting a biological detection material containing the pathogenic microorganisms; extracting nucleic acid in the pathogenic microorganisms to be detected; designing specific primers and probes according to the target pathogenic microorganisms; amplifying a nucleic acid sequence through a PCR (Polymerase Chain Reaction) technology; carrying out deep sequencing based on a high-throughput sequencer; according to the present invention, the specific primers and the probe are designed, such that the nucleic acid sequence of the target pathogenic microorganism can be efficiently enriched, and the high-throughput sequencing technology is adopted to perform the deep sequencing so as to significantly improve the sensitivity and the accuracy of the detection of the plurality of pathogenic microorganisms; the method can be used for simultaneously detecting various different types of pathogenic microorganisms such as bacteria, viruses, fungi and the like, is simple and convenient to operate, short in time consumption and low in cost, and can provide powerful support for quickly and accurately identifying the pathogenic microorganisms and timely taking effective prevention and control measures.

The invention discloses a pathogen detection primer group, a kit and application in eye infection pathogen detection, the detection primer group comprises 162 pairs of primers for 54 pathogenic microorganisms, the interference competition among the primers is small, the 54 pathogenic microorganisms can be detected at the same time by randomly selecting one pair of primers for each pathogenic microorganism, and the detection efficiency is high. When all 162 pairs of primers are selected, the sensitivity and specificity of species identification can be improved, the specific and high-sensitivity detection of bacteria, fungi, viruses, parasites and accurately identified species can be met, and the requirements of clinical accurate diagnosis are met.

The invention relates to a photocatalytic self-cleaning water-based paint and a preparation method thereof, which are applied to the field of paint compositions, and the photocatalytic self-cleaning water-based paint realizes self-decomposition of organic dirt and self-inactivation of pathogenic microorganisms by doping a nano-photocatalyst into a water-based system and utilizing a photocatalytic reaction triggered by light energy, and can purify pollutants in air, so that the photocatalytic self-cleaning water-based paint has the advantages of environmental protection and environmental protection. The formula of the coating is optimized, so that the photocatalyst is uniformly dispersed and stably exists in the coating, the coating not only has efficient action under ultraviolet light, but also has certain activity under visible light conditions, and in addition, the coating is a water-based system, is low in VOCs emission, safe and environment-friendly, and has a good application prospect. The self-cleaning antibacterial air purifying coating is suitable for construction and use in a manned environment, creatively integrates self-cleaning, antibacterial and air purifying functions into a whole so as to solve the problems that in the prior art, outer walls and public environment surfaces are difficult to clean and maintain, and a traditional coating lacks active purifying and antibacterial capabilities, and has important practical application value.

The invention discloses a slow-release antibacterial and antioxidant composite preservative film and a preparation method thereof. The slow-release antibacterial and antioxidant composite preservative film is prepared from a chitosan matrix and Chuzhou chrysanthemum essential oil nano-emulsion loaded in the chitosan matrix, the Chuzhou chrysanthemum essential oil nano-emulsion comprises Chuzhou chrysanthemum essential oil, a surfactant and a cosurfactant, and the average particle size of the Chuzhou chrysanthemum essential oil nano-emulsion is smaller than 200 nm; the composite preservative film has the function of slowly releasing the Chuzhou chrysanthemum essential oil and shows antibacterial and antioxidant activity; the preparation method comprises the following steps: firstly preparing Chuzhou chrysanthemum essential oil into a nano-emulsion by adopting a nano-encapsulation method through a high-pressure homogenization method to form the Chuzhou chrysanthemum essential oil nano-emulsion, then embedding the Chuzhou chrysanthemum essential oil nano-emulsion into chitosan to prepare an active chitosan composite film, and finally applying the antibacterial coating material to food preservation. The antibacterial coating film not only can effectively inhibit pathogenic microorganism infection in berry storage and logistics transportation in a wider spectrum, but also can regulate physiological metabolism of picked berries, delay the senescence process and reduce loss of internal moisture.

The invention relates to a pheretima bombycis farm, and belongs to the technical field of earthworm breeding. The problem that earthworm suffocation death is caused by cross contamination of pathogenic microorganisms and unsmooth drainage due to mixing of functional areas of a traditional farm is solved. The layout of the farm is specifically as follows: a raw material pretreatment and storage area provided with a fermentation tank is arranged in the upwind direction of a site entrance; a core breeding production area is arranged in the center of the field, the interior of the core breeding production area is divided into an earthworm breeding area, a breeding area and a fattening area according to breeding stages, and mechanical channels are arranged in the areas and are of a ridge type structure; arranging an annular drainage ditch along the boundary of the field, and communicating the furrow with the annular drainage ditch by gradient; a finished product treatment and storage area containing an airing field is arranged in the downwind direction of the field exit; an isolation belt is arranged on the periphery, a management and auxiliary area containing a tool room is arranged at an entrance, and a feces treatment area containing a sedimentation tank is arranged in the downwind direction; the hardened main road is connected with all the functional areas, and the branch is communicated with the breeding district. According to the scheme, pollution source isolation and efficient drainage are achieved, and the method is mainly used for improving the earthworm survival rate and breeding benefits.

The invention provides a method for improving storage and preservation of fresh lilies, which comprises the following steps of: soaking the fresh lilies in micro-nano bubble ozone water (O3-MNBW), naturally airing after soaking treatment, and storing under a cold storage condition. The invention also provides a new application of the micro-nano bubble ozone water, namely the application of the micro-nano bubble ozone water in improving the storage quality of the picked fresh lily. According to the method provided by the invention, the weightlessness of the lilium brownii scales is obviously slowed down, the weightlessness, hardness reduction, respiration and browning of the lilium brownii scales are effectively inhibited, the content of soluble solids is maintained, the dip dyeing of pathogenic microorganisms is effectively prevented, and the quality of the lilium brownii scales in the postharvest storage period is obviously improved. Through O < 3 >-MNBW treatment, the sensory evaluation index of the picked fresh lilies is effectively improved, the preservation quality is improved, browning after picking is inhibited, only one-time treatment is needed before storage, and the economic benefit is remarkable. And a novel, economical and effective fresh-keeping mode is provided for the storage period of the picked fruits and vegetables.

The invention relates to the technical field of molecular biology and pathogenic microorganism detection, and discloses a Klebsiella pneumoniae and Escherichia coli synchronous detection method based on RPA-CRISPR / Cas12a and application, and the method comprises the following steps: extracting DNA from a sample, carrying out isothermal amplification by adopting an RPA primer designed aiming at a Klebsiella pneumoniae rcsA gene and an Escherichia coli uriA gene, and carrying out amplification by adopting an RPA primer designed aiming at a Klebsiella pneumoniae RcsA gene and an Escherichia coli uriA gene; a detection system containing Cas12a enzyme, crRNA and a fluorescence report substrate is constructed, and a result is interpreted through a fluorescence or lateral flow chromatography test strip. Detection can be completed within 70 minutes at the constant temperature of 37 DEG C, the sensitivity reaches 5 * 10 copies / mu L, the specificity is high, no cross reaction exists, the kit is suitable for clinical early screening, hospital infection monitoring and on-site POCT application, and an efficient and reliable new tool is provided for pathogen diagnosis.

The invention provides a method of delivering, by intermittent inhalation, 200 ppm to 320 ppm gNO for 10 to 45 minutes 2 to 5 times per day to provide a therapeutic amount of gaseous nitric oxide (gNO) wherein the therapeutic amount is a nitric oxide-load (NO load) of 300 ppm-hrs to 900 ppm-hrs of gNO per day over a period of at least 14 days to a patient in need thereof, wherein the patient has a condition associated with a pathogenic microorganism, wherein the intermittent inhalation comprises inhalation of the gNO for a first time period, followed by inhalation of a gas essentially devoid of gNO for a second time period.

The invention relates to the technical field of pathogenic microorganism detection, in particular to a pathogenic microorganism detection system based on nanopore sequencing, which comprises: a microflow sample introduction module, which uses nanopore sequencing, regulates and controls a microorganism liquid channel and adjusts the opening of a sample introduction valve at the same time, setting the flow rate of the trace liquid and comparing the ion concentration of the conductive pore channel with the pH value of the solution by using a sensor. According to the invention, liquid channel regulation and control are carried out through nanopore sequencing, and accurate control of a fluid environment is realized by combining opening adjustment of a sample introduction valve, flow velocity setting and comparison of ion concentration and pH value of a conductive pore channel, so that a microbial sample is ensured to enter a sequencing system under stable physical and chemical conditions, and the stability of sequencing data is improved. In the cell lysis process, the nucleic acid release rate is adjusted through temperature monitoring, and the particle removal operation is combined, so that the nucleic acid component in the sample is more uniform, and the reliability of subsequent detection is improved.