662 results about "Pathogenic microorganism" patented technology

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention belongs to the technical field of pathogenic microorganism detection, and particularly relates to a sequence combination for detecting cochliobolus miyabeanus based on RPA-CRISPR / Cas12b and application of the sequence combination. The CRISPR / Cas system contained in the composition comprises Cas12b and sgRNA, and the CRISPR / Cas system can be cooperatively used with a specific reaction system. The rapid detection method comprises the following steps: sample pretreatment: treating a sample to extract nucleic acid; performing amplification reaction: performing RPA amplification reaction on the extracted nucleic acid by using the composition, and applying a nucleic acid amplification product to CRISPR / Cas system detection; result interpretation: reading and judging a detection result through a quality control line and a detection line. The composition and the kit provided by the invention are specially used for detecting the cochliobolus miyabeanus. The method is simple and convenient to operate, rapid in detection, short in time consumption, low in cost and suitable for on-site rapid detection.

The invention discloses an identification and analysis method for unknown pathogenic microorganisms, which comprises the following steps: filtering out genome sequences with low integrity, pollution and tag errors, and establishing a high-quality virus identification database; constructing a virus host prediction model through a machine learning algorithm; unknown pathogenic microorganisms are identified and analyzed, potential hosts or pathogenicity of the unknown microorganisms are identified, and whether the unknown microorganisms are unknown pathogenic viruses or bacteria or not is further judged. On the basis of metagenome data analysis, potential unknown pathogenic microorganisms in samples of human bodies, environments and the like can be identified more accurately.

The invention relates to the technical field of soil improvement, and discloses a soil improvement repairing agent and a preparation method thereof. The preparation method comprises the following steps: adding biochar, modified eugenol, modified benzothiazolyl pyridine, bacillus licheniformis and diatomite into a mixer, mixing and stirring, extruding and granulating in a granulator, and drying to obtain the soil improvement repairing agent. Eugenol can destroy a membrane structure and denature protein, so that a bacteriostatic effect is achieved, and a healthy soil microenvironment is provided for crops; the quaternary ammonium salt can destroy the membrane structure of pathogenic microorganisms, plays a role in killing the microorganisms, can improve the antibacterial effect of the remediation agent, and prevents and treats spread diseases of soil; nitrogen atoms and sulfur atoms in benzothiazolyl pyridine molecules can coordinate with free heavy metal ions in the soil, the heavy metal adsorption effect of the remediation agent is improved, the production value of the soil is gradually restored, meanwhile, boric acid capable of being absorbed by plants can be released through hydrolysis of pinacol ester, and the fertility of the soil is improved.

The invention discloses a respiratory infectious disease multi-pathogen nucleic acid detection kit based on a fluorescent PCR detection technology and application of the respiratory infectious disease multi-pathogen nucleic acid detection kit. The invention provides two detection kits aiming at 20 and 6 respiratory tract detection, the two kits can specifically detect respiratory tract pathogens, the detection sensitivity is high, the whole detection system is short in time consumption and simple and convenient in use process, and detection results of 20 pathogenic microorganisms can be reported within 40 min at the shortest; the whole set of detection kit and the detection method are high in sensitivity and good in specificity; the normal-temperature storage and transportation of the reagent can be realized.

The invention discloses a primer combination for identifying six genital tract pathogens and detecting drug resistance genes, a kit and application, the primer combination comprises a primer combination for identifying chlamydia trachomatis, mycoplasma genitalium, ureaplasma urealyticum, mycoplasma hominis, neisseria gonorrhoeae and trichomonas vaginalis and detecting the drug resistance genes of the six genital tract pathogens, and nucleotide sequences are shown as SEQ ID NO.1-SEQ ID NO.50. Microorganisms and related drug-resistant genes in a detection range are identified by utilizing a multiple PCR targeted three-generation sequencing method, the specificity is good, the sensitivity is high, the detection technology has important clinical significance when being applied to identification of genital tract infection pathogens, and compared with a traditional detection method, the detection flux, the coverage range and the sensitivity are higher, and detection is stable; compared with pathogenic microorganism metagenome (mNGS) detection, the method has the advantages that the detection cost is lower, and the detection sensitivity on drug-resistant genes and drug-resistant gene mutation is higher.

The invention relates to the technical field of biological detection, and particularly discloses a farm animal excrement pathogenic microorganism automatic sampling detection method, which comprises the following steps: integrating and analyzing farm three-dimensional geographic information, historical epidemic situation data and real-time environmental parameters by using a deep learning algorithm, and generating a pathogenic microorganism risk thermodynamic diagram; driving an autonomous navigation vehicle equipped with a multispectral sensor, performing dynamic path planning according to the risk thermodynamic diagram, and preferentially collecting an excrement sample in a high-risk area; through the risk thermodynamic diagram generated by a deep learning algorithm, accurate positioning of a sampling area is realized, blind sampling is reduced, the sampling process is autonomously completed in combination with an autonomous navigation vehicle, flexible operation can be performed in a complex environment, efficient execution of a sampling task is ensured, and meanwhile, due to the use of a bionic micro-fluidic chip, the sampling efficiency is improved. Not only is high-efficiency separation of sticky substances and microorganisms realized, but also the recovery rate of pathogenic microorganisms is greatly improved.

The present invention relates to a fungicidal composition comprising components (A) and (B), wherein components (A) and (B) are as defined in claim 1; and the use of the composition in agriculture or horticulture for controlling or preventing infestation of plants by phytopathogenic microorganisms, preferably fungi.

The invention discloses a pathogen detection primer group, a kit and application in eye infection pathogen detection, the detection primer group comprises 162 pairs of primers for 54 pathogenic microorganisms, the interference competition among the primers is small, the 54 pathogenic microorganisms can be detected at the same time by randomly selecting one pair of primers for each pathogenic microorganism, and the detection efficiency is high. When all 162 pairs of primers are selected, the sensitivity and specificity of species identification can be improved, the specific and high-sensitivity detection of bacteria, fungi, viruses, parasites and accurately identified species can be met, and the requirements of clinical accurate diagnosis are met.

The invention discloses a slow-release antibacterial and antioxidant composite preservative film and a preparation method thereof. The slow-release antibacterial and antioxidant composite preservative film is prepared from a chitosan matrix and Chuzhou chrysanthemum essential oil nano-emulsion loaded in the chitosan matrix, the Chuzhou chrysanthemum essential oil nano-emulsion comprises Chuzhou chrysanthemum essential oil, a surfactant and a cosurfactant, and the average particle size of the Chuzhou chrysanthemum essential oil nano-emulsion is smaller than 200 nm; the composite preservative film has the function of slowly releasing the Chuzhou chrysanthemum essential oil and shows antibacterial and antioxidant activity; the preparation method comprises the following steps: firstly preparing Chuzhou chrysanthemum essential oil into a nano-emulsion by adopting a nano-encapsulation method through a high-pressure homogenization method to form the Chuzhou chrysanthemum essential oil nano-emulsion, then embedding the Chuzhou chrysanthemum essential oil nano-emulsion into chitosan to prepare an active chitosan composite film, and finally applying the antibacterial coating material to food preservation. The antibacterial coating film not only can effectively inhibit pathogenic microorganism infection in berry storage and logistics transportation in a wider spectrum, but also can regulate physiological metabolism of picked berries, delay the senescence process and reduce loss of internal moisture.

The invention relates to a pheretima bombycis farm, and belongs to the technical field of earthworm breeding. The problem that earthworm suffocation death is caused by cross contamination of pathogenic microorganisms and unsmooth drainage due to mixing of functional areas of a traditional farm is solved. The layout of the farm is specifically as follows: a raw material pretreatment and storage area provided with a fermentation tank is arranged in the upwind direction of a site entrance; a core breeding production area is arranged in the center of the field, the interior of the core breeding production area is divided into an earthworm breeding area, a breeding area and a fattening area according to breeding stages, and mechanical channels are arranged in the areas and are of a ridge type structure; arranging an annular drainage ditch along the boundary of the field, and communicating the furrow with the annular drainage ditch by gradient; a finished product treatment and storage area containing an airing field is arranged in the downwind direction of the field exit; an isolation belt is arranged on the periphery, a management and auxiliary area containing a tool room is arranged at an entrance, and a feces treatment area containing a sedimentation tank is arranged in the downwind direction; the hardened main road is connected with all the functional areas, and the branch is communicated with the breeding district. According to the scheme, pollution source isolation and efficient drainage are achieved, and the method is mainly used for improving the earthworm survival rate and breeding benefits.

The invention provides a method for improving storage and preservation of fresh lilies, which comprises the following steps of: soaking the fresh lilies in micro-nano bubble ozone water (O3-MNBW), naturally airing after soaking treatment, and storing under a cold storage condition. The invention also provides a new application of the micro-nano bubble ozone water, namely the application of the micro-nano bubble ozone water in improving the storage quality of the picked fresh lily. According to the method provided by the invention, the weightlessness of the lilium brownii scales is obviously slowed down, the weightlessness, hardness reduction, respiration and browning of the lilium brownii scales are effectively inhibited, the content of soluble solids is maintained, the dip dyeing of pathogenic microorganisms is effectively prevented, and the quality of the lilium brownii scales in the postharvest storage period is obviously improved. Through O < 3 >-MNBW treatment, the sensory evaluation index of the picked fresh lilies is effectively improved, the preservation quality is improved, browning after picking is inhibited, only one-time treatment is needed before storage, and the economic benefit is remarkable. And a novel, economical and effective fresh-keeping mode is provided for the storage period of the picked fruits and vegetables.

The invention relates to the technical field of molecular biology and pathogenic microorganism detection, and discloses a Klebsiella pneumoniae and Escherichia coli synchronous detection method based on RPA-CRISPR / Cas12a and application, and the method comprises the following steps: extracting DNA from a sample, carrying out isothermal amplification by adopting an RPA primer designed aiming at a Klebsiella pneumoniae rcsA gene and an Escherichia coli uriA gene, and carrying out amplification by adopting an RPA primer designed aiming at a Klebsiella pneumoniae RcsA gene and an Escherichia coli uriA gene; a detection system containing Cas12a enzyme, crRNA and a fluorescence report substrate is constructed, and a result is interpreted through a fluorescence or lateral flow chromatography test strip. Detection can be completed within 70 minutes at the constant temperature of 37 DEG C, the sensitivity reaches 5 * 10 copies / mu L, the specificity is high, no cross reaction exists, the kit is suitable for clinical early screening, hospital infection monitoring and on-site POCT application, and an efficient and reliable new tool is provided for pathogen diagnosis.

The invention discloses a pathogenic microorganism detection system based on nanopore sequencing and intelligent interpretation. The system comprises six modules including a biomolecule electric signal acquisition module and a pathogen characteristic sequence extraction module. Ion current change signals are collected through a nanopore chip array, a pathogen characteristic sequence is extracted through differential coding, a hypergraph topological structure self-adaption building module is used for building a pathogen relation model, a multi-dimensional heterogeneous pathogen parameter fusion analysis module integrates morphological parameters, metabonomics parameters and other multi-dimensional parameters, and the multi-dimensional heterogeneous pathogen parameter fusion analysis module is used for analyzing the multi-dimensional parameters. The optimized hypergraph neural network reasoning module realizes pathogen category prediction, and finally, the nanopore sequencing result intelligent interpretation output module generates a detection report containing pathogen categories, subtypes and risk levels. The system is combined with nanopore sequencing and an intelligent algorithm, pathogenic microorganism detection can be efficiently and accurately completed, the accuracy, reliability and efficiency of detection are greatly improved, and the requirement for rapid and accurate detection of pathogens in practical application is met.

The invention discloses a microbial odor remover suitable for environment and a preparation method thereof, and relates to the technical field of daily chemical products. The preparation method comprises the following steps: adding pediococcus acidilactici and bacillus velezensis into a citric acid-disodium hydrogen phosphate buffer solution according to a ratio, standing and activating, adding bromelain, lipase, rosmarinic acid nanoemulsion, tea polyphenol and citrus polyphenol-EDTA (Ethylene Diamine Tetraacetic Acid) mixture, homogenizing, adding konjac glucomannan-chitosan gel and a trehalose-glycerol compound according to a ratio, stirring, filtering, and drying to obtain a finished product. And subpackaging in a dark place to prepare the microbial odor remover suitable for the environment. Through the synergistic effect of microorganisms, enzymes and a natural deodorization medium, a multi-stage peculiar smell elimination system is constructed, the dual functions of immediate neutralization and continuous bacteriostasis are achieved, the application scene is wide, corrosion and residues to base materials such as fabric and plastic are avoided, and the concentration of harmful gas can be more effectively controlled and pathogenic microorganisms can be inhibited in large-scale breeding application.

Disclosed herein are compositions and methods for using human-derived Janthinobacterium lividum. Compositions improve skin health. Methods may include applying human-derived Janthinobacterium lividum over a host or host area, such as skin or mucosa, to minimize the presence of one or more microbes, maximize therapeutic effects, and / or improve health. A method to minimize a pathogenic microbe may include applying to a surface a composition including human-derived Janthinobacterium lividum and an acceptable carrier. Compositions and methods may include a prebiotic to maximize growth and / or metabolites. Compositions and methods may include human-derived Janthinobacterium lividum metabolites, such as violacein, prodigiosin, indole-3-carboxaldehyde, and lantibiotics, and / or other Postbiotics.

A biofilm shock treatment includes: a zinc ion source and a disinfecting agent. The biofilm shock treatment is configured to weaken a matrix of a biofilm and to disinfect causative microbes while promoting biofilm removal. Optionally, the biofilm shock may include a surfactant and / or a chelating agent.

The invention relates to a silver-iron diatom modified carbon nitride (AgFe-CN) photocatalytic material capable of synchronously removing organic pollutants and pathogenic bacteria as well as a preparation method and application of the silver-iron diatom modified carbon nitride (AgFe-CN) photocatalytic material, and belongs to the field of environmental chemistry and material science. The catalyst is prepared through a supramolecular coordination-thermal polymerization method, wherein silver and iron are highly dispersed on a porous carbon nitride substrate in a monatomic form. The monatomic Ag and Fe synergistically construct an electron channel, and jointly endow the catalyst with excellent photocatalytic activity. The preparation method comprises the following steps: combining the catalyst with peroxymonosulfate (PMS) to construct an AgFe-CN / PMS / Vis photocatalytic Fenton-like synergistic system; in the system, the catalyst can lead generation of an advanced oxidation system which takes singlet oxygen as a main component and cooperates with superoxide free radicals and high-valence iron oxygen species, so that efficient and synchronous removal of various organic pollutants and pathogenic microorganisms in the water body is realized. The catalyst is simple in preparation process and mild in condition, and the constructed water treatment method is high in efficiency, high in universality and good in stability and has wide application prospects.

The invention belongs to the technical field of microbiological detection, and particularly relates to a primer group and a kit for simultaneously detecting multiple pathogenic microorganisms and application of the primer group and the kit in detection of mouse-borne pathogenic microorganisms. The nucleotide sequences of the primer group are as shown in SEQ ID NO: 1 to SEQ ID NO: 122. In each PCR detection system, when the template quantity reaches 20 copies or more concentration, 22 pathogenic microorganism mock plasmids can be completely detected, which indicates that the primer group can effectively detect pathogenic microorganisms in mice and parasites carried by the mice, and the detection sensitivity is equivalent to that of PCR. Meanwhile, the detection method has the advantages of rapidness, high efficiency, low cost, wide and accurate detection range and capability of meeting the requirements of high sensitivity and low sample size.

The invention relates to the technical field of blood culture bottles, in particular to a composition and application thereof in preparation of a microbial culture medium. Yeast extract powder, soy peptone and casein tryptone are combined into a nitrogen source, and an aerobic and anaerobic culture medium for clinical pathogenic microorganism culture is formed. Through comparison verification, in growth experiments of nine aerobic bacteria and seven anaerobic bacteria, the culture speed and the biomass accumulation of the aerobic and anaerobic culture media added with the composition are integrally superior to those of other components excluded in the prior art and screening. Therefore, the composition can effectively promote the performance of a blood culture product, improve the clinical blood flow infection detection capability, inhibit antibiotic abuse and save the life of a patient.

The present invention discloses a method and device for preventing and controlling soil-borne diseases by burning crop stubble. The method comprises the following steps: S1. cutting and collecting the shallow soil layer where the stubble is located; S2. separating and processing the collected shallow soil layer, removing the soil carried by the stubble, and obtaining separated stubble and soil; S3. burning the separated stubble, and heating the separated soil with the high-temperature flue gas generated by the combustion; S4. mixing the ash from the burned stubble with the heated soil, and then returning the ash to the field. The present application utilizes the high calorific value characteristics of crop stubble, and the high-temperature flue gas generated by its combustion heats the surface soil, thereby killing the stubble and the diseased and insect eggs and pathogenic microorganisms in the rhizosphere soil carried by the stubble. It can also form a certain thermal contact killing effect on the diseases and insect pests in the soil, reduce the degree of damage caused by the diseases and insect pests, and thus achieve the purpose of green prevention and control of diseases and insect pests.

The invention belongs to the technical field of pathogenic microorganism screening, and particularly relates to vibrio parahaemolyticus capable of causing prawn vibrio vitreovibriosis and application of the vibrio parahaemolyticus, and the pathogenic bacteria can be used for preparing inactivated vaccines or used as the pathogenic bacteria to screen medicine components with the effect of preventing and treating the prawn vibrio vitreovibriosis or a culture method. The preservation number of the vibrio parahaemolyticus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) The pathogenicity of the vibrio parahaemolyticus with high pathogenicity and strong lethality is about 1000 times stronger than that of the vibrio parahaemolyticus which is collected by the world animal hygiene organization and causes acute hepatopancreatic necrosis, and the strain is popular in main prawn culture areas and causes serious harm to the prawn fry culture industry in China. The vibrio parahaemolyticus provided by the invention can be used as a positive control for preparing a diagnosis product for the prawn vibrio vitreovirus disease; or preparing an immune vaccine after inactivation.

The invention provides a small molecule compound capable of inducing plant immune response and improving plant disease resistance. Specifically, it is found for the first time that the 2'c ADPR shown in the formula I or the analogue thereof, or the pharmaceutically acceptable salt thereof can significantly enhance the resistance of plants to pathogenic microorganisms, diseases and pests and viruses.

The invention discloses a protein fiber-iron phytic acid composite flocculant as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out hydrolysis reaction on protein powder under an alkaline condition, and then cross-linking through epichlorohydrin to obtain epoxy group functionalized protein fibers; reacting phytic acid with an Fe salt precursor to prepare an Fe-phytic acid complex; and reacting the epoxy group functionalized protein fiber with the Fe-phytic acid complex to obtain the composite flocculant. The composite flocculant is a nano composite material with a three-dimensional network structure and a high specific surface area, has excellent flocculating settling capacity and broad-spectrum bactericidal performance, and can synchronously realize stable sludge structure, pathogenic microorganism control and ecological toxicity reduction in a sewage treatment system; the method effectively replaces traditional agents with potential safety hazards such as aluminum salt, ferric salt and chlorine-containing bactericide, and shows good adaptability and engineering feasibility under the condition of coping with high organic load and complex microbial systems.

According to the plasma high-efficiency water purification system and method suitable for factory-like circulating aquaculture, a water purification unit with a plasma secondary reaction technology as a core is constructed, and a low-energy-consumption cold plasma generator is used for pre-activating gas and introducing the gas into a water body in a micro-nano bubble form; further, an underwater SDBD cold plasma generator is utilized to secondarily excite the activated bubbles to obtain underwater micro-plasmas, and short-life ROS (reactive oxygen species) mainly comprising. OH is efficiently generated through a gas-liquid interface micro-discharge effect, so that pathogenic microorganisms and harmful components such as ammonia nitrogen and nitrite in aquaculture sea and fresh water are quickly and efficiently purified; an electrochemical reaction technology and an internal circulation technology are utilized to construct a plasma by-product elimination unit, the electrochemical reaction technology enables the biotoxicity of a water body to be rapidly reduced to a safe level, and the internal circulation technology avoids the problem of plasma reaction tail gas pollution.

The invention belongs to the technical field of bacterium detection, and discloses a multiple PCR primer for simultaneously detecting eight respiratory tract pathogenic bacteria and application thereof. On the basis of a Luminex liquid phase chip technology, specific primers are designed aiming at eight common respiratory tract infection pathogenic bacteria including pseudomonas aeruginosa, staphylococcus aureus, streptococcus pneumoniae, klebsiella pneumoniae, acinetobacter baumannii, stenotrophomonas maltophilia, haemophilus influenzae and escherichia coli; the multiple PCR primers which are high in amplification efficiency, good in specificity and suitable for simultaneously detecting the eight respiratory tract pathogenic bacteria are screened out, the multiple PCR system and microsphere hybridization conditions are continuously optimized, the multiple PCR method for the eight respiratory tract pathogenic bacteria is successfully constructed by applying the Luminex xTAG technology, and the multiple PCR method can be applied to rapid detection of multiple pathogenic microorganisms.

The invention relates to the technical field of pathogenic microorganism detection, and particularly discloses a primer group, a detection kit and a detection method for detecting pathogenic microorganisms of rodent experimental animals. The primer group disclosed by the invention is used for simultaneously detecting pasteurella pneumophila, klebsiella pneumoniae and staphylococcus aureus; the primer group comprises a primer pair aiming at pasteurella pneumophila, and the sequences of the primer pair are SEQ ID NO: 1 and SEQ ID NO: 2; the sequences of the primer pair aiming at the klebsiella pneumoniae are SEQ ID NO: 7 and SEQ ID NO: 8; the sequences of the primer pair aiming at the staphylococcus aureus are SEQ ID NO: 9 and SEQ ID NO: 10. The primer group and the detection method disclosed by the invention can realize rapid detection of three bacteria on a molecular level, have the advantages of simplicity and convenience in operation, high detection efficiency and accurate result, and are suitable for rapid screening and monitoring of health conditions of experimental animals.

The invention provides a fruit juice fermentation inoculant, fermented guava juice and a preparation method, and belongs to the technical field of fermented beverages. The invention provides a compound fermentation inoculant capable of fermenting fruit juice, the compound fermentation inoculant is composed of lactobacillus plantarum and lactobacillus fermentum, the fermentation inoculant is applied to fermentation of guava fruit juice, beta-glucosidase secreted in the fermentation process can hydrolyze combined polyphenol substances in guavas, and then the guava fruit juice is fermented. The antioxidant is converted into high-activity antioxidants such as quercetin and gallic acid in a free state, so that the content of free phenol is increased by about 2 times, and the scavenging rate of DPPH free radicals is increased by more than 40%. The fermentation is carried out in an oxygen-free environment, the retention rate of vitamin C can reach 80% or above, the low pH value at the fermentation end point and organic acid jointly inhibit pathogenic microorganisms, and the product stability is guaranteed. Therefore, the guava juice prepared from the compound fermentation inoculant maximally releases the natural nutritional potential of guavas.

The invention relates to the technical field of food biology, and discloses an antiviral cubilose polysaccharide active component enriched with bound sialic acid as well as a preparation method and application of the antiviral cubilose polysaccharide active component. The weight-average molecular weight of the cubilose polysaccharide active component is 30-36 kDa, the molecular weight distribution coefficient is 1.1-1.2, the content of bound sialic acid is greater than or equal to 24.0%, and the content of total sugar is greater than or equal to 57.0%. The preparation method comprises the following steps: pretreating a cubilose raw material in a boiling water bath, and sequentially carrying out alkaline protease and neutral protease enzymolysis; carrying out alcohol precipitation on supernate, redissolving, and carrying out papain enzymolysis; and after enzyme deactivation, adjusting the ethanol concentration to 75-85%, precipitating, and drying. According to the method, the cubilose polysaccharide can be efficiently enriched, the combined sialic acid structure of the cubilose polysaccharide is reserved, and the obtained component has the effects of resisting oxidation, inhibiting pathogenic microorganisms and regulating intestinal microecology.

The invention provides a device for collecting oral pathogenic microorganisms. The device comprises a throat swab, the throat swab further comprises a straight pipe, a clamping assembly is arranged in one end of the straight pipe, and the clamping assembly is used for clamping the rod portion of the throat swab. The clamping assembly is provided with an unlocking part; the pressing head is arranged outside the end, away from the clamping assembly, of the straight pipe, and the pressing head is connected with the unlocking part of the clamping assembly through a connecting rod; the protective cover is arranged on the outer side of the end, clamping the throat swab, of the straight pipe in a sleeving mode, an inserting assembly is arranged on the protective cover, and the inserting assembly is connected with an inserting groove formed in the outer wall of the straight pipe in an inserting mode. The straight tube is used as an extended holding part of the throat swab, so that the throat swab does not need an overlong rod part, and the rod part of the throat swab does not need to be broken after the throat swab is arranged in the sampling tube. Furthermore, a clamping assembly is arranged in the straight pipe and used for clamping the throat swab, the throat swab can be clamped and loosened only by pressing a pressing head, and the throat swab and the straight pipe can be connected and separated conveniently.