322 results about "Pathogenic microorganism" patented technology

The invention relates to a silver-iron diatom modified carbon nitride (AgFe-CN) photocatalytic material capable of synchronously removing organic pollutants and pathogenic bacteria as well as a preparation method and application of the silver-iron diatom modified carbon nitride (AgFe-CN) photocatalytic material, and belongs to the field of environmental chemistry and material science. The catalyst is prepared through a supramolecular coordination-thermal polymerization method, wherein silver and iron are highly dispersed on a porous carbon nitride substrate in a monatomic form. The monatomic Ag and Fe synergistically construct an electron channel, and jointly endow the catalyst with excellent photocatalytic activity. The preparation method comprises the following steps: combining the catalyst with peroxymonosulfate (PMS) to construct an AgFe-CN / PMS / Vis photocatalytic Fenton-like synergistic system; in the system, the catalyst can lead generation of an advanced oxidation system which takes singlet oxygen as a main component and cooperates with superoxide free radicals and high-valence iron oxygen species, so that efficient and synchronous removal of various organic pollutants and pathogenic microorganisms in the water body is realized. The catalyst is simple in preparation process and mild in condition, and the constructed water treatment method is high in efficiency, high in universality and good in stability and has wide application prospects.

The invention relates to the technical field of food biology, and discloses an antiviral cubilose polysaccharide active component enriched with bound sialic acid as well as a preparation method and application of the antiviral cubilose polysaccharide active component. The weight-average molecular weight of the cubilose polysaccharide active component is 30-36 kDa, the molecular weight distribution coefficient is 1.1-1.2, the content of bound sialic acid is greater than or equal to 24.0%, and the content of total sugar is greater than or equal to 57.0%. The preparation method comprises the following steps: pretreating a cubilose raw material in a boiling water bath, and sequentially carrying out alkaline protease and neutral protease enzymolysis; carrying out alcohol precipitation on supernate, redissolving, and carrying out papain enzymolysis; and after enzyme deactivation, adjusting the ethanol concentration to 75-85%, precipitating, and drying. According to the method, the cubilose polysaccharide can be efficiently enriched, the combined sialic acid structure of the cubilose polysaccharide is reserved, and the obtained component has the effects of resisting oxidation, inhibiting pathogenic microorganisms and regulating intestinal microecology.

The invention provides a device for collecting oral pathogenic microorganisms. The device comprises a throat swab, the throat swab further comprises a straight pipe, a clamping assembly is arranged in one end of the straight pipe, and the clamping assembly is used for clamping the rod portion of the throat swab. The clamping assembly is provided with an unlocking part; the pressing head is arranged outside the end, away from the clamping assembly, of the straight pipe, and the pressing head is connected with the unlocking part of the clamping assembly through a connecting rod; the protective cover is arranged on the outer side of the end, clamping the throat swab, of the straight pipe in a sleeving mode, an inserting assembly is arranged on the protective cover, and the inserting assembly is connected with an inserting groove formed in the outer wall of the straight pipe in an inserting mode. The straight tube is used as an extended holding part of the throat swab, so that the throat swab does not need an overlong rod part, and the rod part of the throat swab does not need to be broken after the throat swab is arranged in the sampling tube. Furthermore, a clamping assembly is arranged in the straight pipe and used for clamping the throat swab, the throat swab can be clamped and loosened only by pressing a pressing head, and the throat swab and the straight pipe can be connected and separated conveniently.

The application discloses a bucket type cultivation method for overcoming continuous cropping obstacles of bamboo shoots. Through the bucket type inoculation, the mycelium is effectively isolated from the miscellaneous bacteria and pathogenic microorganisms in the soil before the mycelium completes the dominant expansion, the mycelium expansion stability is obviously improved, and the mycelium death and yield loss caused by the continuous cropping obstacles are avoided. Field tests show that the yield of the second year can be recovered to more than 90% of the yield of the first year by using the method, and the yield of the second year is less than 10% of the yield of the traditional reseeding mode, and almost no yield is obtained, so the method has good stability and generalizability.

The process for producing and using polymeric Hydrocells for production of atmospheric water using a superabsorbent polymer derived from alkaline hydrolysis of the polyacrylonitrile (PAN). The Hydrocell adsorbs up to 80% of its weight in atmospheric water under ambient conditions and releases this water when heated to less than 100° C. using renewable energy sources, such as solar energy. This low-temperature operation allows for repeated adsorption and desorption cycles lasting years without degradation. Multiple Hydrocells can be arranged into Hydrobatteries with a surface area greater than 2 m2, capable of adsorbing several kg of water per day. The Hydrocells offer a sustainable and safe solution for producing potable water free of toxic chemicals and pathogenic microorganisms in regions with limited water resources. By drawing water from the vast atmospheric reservoir containing over 13,000 km3, this can help prevent deaths caused by the consumption of contaminated water.

This invention belongs to the field of paper disinfection technology, and particularly relates to a gas disinfection device for preventing the spread of pathogenic microorganisms. The device includes a disinfection chamber, a directional paper clamp, a side clamp assembly, a jet pipe, an opening assembly, and a control terminal. In this invention, the directional paper clamp holds the paper, and the jet pipe located below the directional paper clamp sprays disinfecting gas from the bottom of the paper, allowing the disinfecting gas to gradually spread upwards, thus disinfecting the paper. When the directional paper clamp passes through the flipping zone, it rotates the paper 180°. After flipping, the clamping plate and side support plate below stop holding the paper, allowing subsequent disinfecting gas to be blown onto the paper, thus fully disinfecting it and preventing the area of ​​the paper held by the clamping plate and side support plate from being left undisinfected.

The invention belongs to the technical field of biological detection, and provides a kit for detecting EGFR (epidermal growth factor receptor) 19del subtypes. According to the invention, by designing four universal upstream primer target segments and four probes combined with the upstream primers, different subtypes of EGFR19del can be accurately detected by a Tm value; by changing the fluorophore combination of the probe, single-tube multi-channel accurate typing can be realized. The primer tag segment and detection probe combination developed by the invention is not only suitable for deletion mutation, but also suitable for detection of fusion mutation and pathogenic microorganisms, and has wide applicability.

The application provides a preparation method of a multi-layer sandwich type epoxy group modified magnetic microsphere and application thereof. The multi-layer sandwich type epoxy magnetic microsphere prepared by the preparation method provided in the application can realize efficient and accurate cell sorting through coupling of an antibody, is suitable for cell sorting related scenes such as stem cell separation, tumor cell detection, immune cell treatment (such as CAR-T cell preparation) and pathogenic microorganism enrichment, and provides key material support for cell sorting technology in life science research and clinical diagnosis and treatment.

The invention discloses a drinking water targeted sterilization and disinfection device based on an asymmetric vacancy catalytic material and application of the drinking water targeted sterilization and disinfection device to removal of pathogenic microorganism polluted water, and relates to the technical field of water treatment. The device comprises a raw water tank, a peristaltic pump, a filter column, an aeration pump, an aeration diffuser and a clean water tank, the filter column comprises a column body, an asymmetric vacancy catalytic material, a bearing plate, a gravel filler and an oyster shell filler, a bearing plate is arranged at the bottom of the column body, and a gravel filler, an oyster shell filler and an asymmetric vacancy catalytic material are sequentially arranged on the bearing plate from bottom to top. According to the invention, an asymmetric vacancy catalytic material is precisely constructed, an electron polarization effect is induced, and a filter column drinking water treatment device is combined, so that a novel RO / 1O2 synergistic oxidation disinfection technology which is efficient, targeted and low in byproduct generation is constructed. Safety guarantee is provided for rural distributed water supply, the safety and reliability of rural drinking water can be comprehensively improved, and sustainable development of rural drinking water safety engineering is promoted.

The invention belongs to the technical field of bioinformatics, and particularly discloses a pathogenic microorganism specific primer design method and device. The pathogenic microorganism specific primer design method provided by the invention adopts an original design thought and comprises the following steps: firstly, carrying out genome investigation by using K-mer, and after a conservative sequence region of a target species is obtained, according to a coordinate position of the region on a reference genome and a designed length of an amplification product, determining the specific primer of the pathogenic microorganism according to the coordinate position of the region on the reference genome and the designed length of the amplification product. On the premise that the sequence consistency of an amplification area is not required, conservative upstream and downstream primer design areas are obtained, then the conservative upstream and downstream primer design areas and a specific sequence area are overlapped, the overlapped area is a primer design area conforming to conservative and specific requirements at the same time, then candidate primers conforming to the requirements are output through primer design software, and the candidate primers are used for detecting the sequence consistency of the amplification area. And carrying out coverage and specificity evaluation on the candidate primers to obtain the specific primers of the target species. The method has the advantages of high analysis speed and high precision, can be compatible with pathogen genomes with different characteristics, and improves the success rate of primer design.

The application provides bacillus velezensis LB17, K87, MP6 and a compound microbial inoculant thereof, a preparation method and application, and belongs to the technical field of biocontrol bacteria.The application collects rhizosphere and rhizosphere soil of characteristic crops such as highland barley, lily, tomato and pepper in the Gansu-Qinghai region, and obtains 305 pure cultures through separation, obtains three strains of bacillus velezensis LB17, K87 and MP6 through multiple screening, and finally obtains a compound fermentation microbial inoculant, i.e., tuoweiwei No.7, which is fermented by the three strains of bacillus velezensis LB17, K87 and MP6 through optimization of inoculation amount, components of culture medium, ratio and fermentation conditions.Tuoweiwei No.7 can effectively prevent plant diseases such as pathogenic microorganisms, root rot and fusarium wilt, and can promote plant growth and improve the stress resistance of plants.The compound microbial inoculant provided by the application provides a strong guarantee for the prevention and control of root rot diseases of special habitat crops in the Gansu-Qinghai region.

The invention belongs to the technical field of molecular biology, and particularly relates to a pathogenic microorganism nucleic acid co-extraction kit, an extraction method and application. The invention provides a lysis solution for nucleic acid co-extraction of pathogenic microorganisms. The lysis solution comprises a first guanidine salt, an ionic surfactant, a first sodium salt and a first nonionic surfactant. According to the splitting system, guanidine salt is adopted and matched with a certain content of sodium salt and a surfactant, a sample is split and precipitated under the grinding condition, nucleic acid cannot be damaged in the crushing process, and pathogenic microorganism DNA and RNA with high concentration and complete fragments can be extracted.

The present invention relates to formula (I) [Formula 1] JPEG2026514064000113.jpg58160 (wherein the formula, the substituents are as defined in claim 1) The present invention relates to a compound of formula (I), a process and method for preparing a compound of formula (I), a pesticide composition comprising a compound of formula (I) as defined in claim 1, the preparation of these compositions, and the use of the compound or composition in agriculture or horticulture for controlling, preventing or controlling ectoparasitism of plants, harvested food crops, seeds or non-biological materials by plant pathogenic microorganisms, particularly fungi.

The application relates to the technical field of biological medicine, and particularly discloses CpG oligodeoxynucleotides and application thereof. The CpG oligodeoxynucleotide has a sequence as shown in any one of SEQ ID NO. 3-6, 9, and preferably has a sequence as shown in SEQ ID NO. 3-6. The CpG oligodeoxynucleotide can effectively activate the immune system of a human body or an animal, has the functions of preventing and treating pathogenic microorganism infection in a broad spectrum, has the function of improving the immunogenicity of a common vaccine, and can be applied as a human or animal vaccine adjuvant.

The invention relates to the technical field of computer vision and intelligent medical examination, in particular to an animal pathogenic microorganism target image detection model construction system which comprises a sample collection module used for controlling digital microscopic imaging equipment to collect an original microscopic image matrix containing target microorganisms; the ideal reference construction module is used for constructing an ideal reference light field image matrix; the knowledge parameterization injection module is used for generating a theoretical simulation state image matrix, a biological factor tensor for controlling geometric deformation and a flaking factor tensor for controlling pixel intensity attenuation; the double-track difference module is used for generating a real light field residual matrix and a theoretical simulation residual matrix; the coupling judgment and model construction module is used for calculating the Euclidean distance of the feature vectors of the two residual matrixes; according to the method, rapid and accurate inversion from real observation to theoretical parameters is realized, and the efficiency of model construction and iteration is greatly improved.

The invention relates to the technical field of pathogenic microorganism detection, and discloses a helicobacter pylori detection kit based on a urease method and a preparation method, the helicobacter pylori detection kit comprises a detection sleeve card, detection test paper and a tartar sampling label, the test paper is soaked in a reaction solution and a buffer solution in the preparation process of the detection test paper, the pH of the buffer solution is 6.5-7.0, and the tartar sampling label is used for sampling tartar. The buffer solution comprises monopotassium phosphate and dipotassium phosphate, the final concentration of the monopotassium phosphate is 14-30 g / L, and the final concentration of the dipotassium phosphate is 1.8-3.6 g / L. The method can solve the problems of high false positive rate of detection results, poor accuracy, slow reagent response speed and the like caused by complex oral environment (such as diet residue and non-specific bacterial interference) and lack of an effective control system when a tartar sample is used for detecting helicobacter pylori in the prior art.

The invention discloses a traditional Chinese medicine composition for treating chicken enteritis. The traditional Chinese medicine composition is prepared by fermenting traditional Chinese medicine raw materials through probiotics, the traditional Chinese medicine raw materials comprise the following components in parts by weight: ovateleaf holly bark, rhizoma cyperi, pomegranate rind and liquorice, the invention also discloses a preparation method of the traditional Chinese medicine composition, and the preparation method comprises the following steps: S1, crushing the ovateleaf holly bark and sieving for later use, respectively crushing the rhizoma cyperi, the pomegranate rind and the liquorice, sieving and mixing to obtain a solid matrix for later use; s2, adding ethanol into the crushed ovateleaf holly bark powder for reflux extraction to obtain an ovateleaf holly bark alcohol extract; s3, diluting the ovateleaf holly bark alcohol extract, and adding the diluted ovateleaf holly bark alcohol extract into the solid matrix to obtain a soft material; s4, the soft material is sterilized and then cooled; s5, bacillus liquid, lactic acid bacteria liquid and saccharomycetes liquid are taken and sprayed to the surface of the soft material, and the soft material is placed in a fermentation box to be fermented; and S6, carrying out multi-stage fermentation in a fermentation box, taking out, drying, crushing and sieving after the multi-stage fermentation is finished, thereby obtaining the product. The chicken enteritis caused by pathogenic microorganisms can be effectively treated, and the economic benefit of breeding is improved.

The application discloses a nano spider web composite ordered nanofiber air filtration membrane and a preparation method and application thereof. The filtration membrane comprises a multi-stage pore structure and is stable in structure, and is a composite ordered nanofiber membrane capable of completely filtering particulate matters and pathogenic microorganisms through physical interception. The application adopts a polymer solution as a spinning solution, and adopts an electrospinning technology to prepare an ordered nanofiber membrane, and then adopts an electrostatic spraying technology to composite a layer of nano spider web on the surface of the ordered nanofiber membrane. The application improves the filtration efficiency of the nanofiber membrane while ensuring a low pressure drop, and improves the filtration performance of the air filtration membrane.

The invention belongs to the field of preparation of high-antibacterial carbon dots, and discloses a high-antibacterial carbon dot, a preparation process and application of the high-antibacterial carbon dot in vaginal gel. The prepared carbon dots are prepared by adopting physical shearing and ultrasonic synergistic pretreatment, then cooperating with microwave gradient energy crushing and stripping, and then performing centrifugation, dialysis purification and vacuum freeze drying, the damage of oxidation or acid treatment to a graphite structure is avoided in the whole process, and the carbon dots have excellent antibacterial and antiviral performance, can effectively inactivate various pathogenic microorganisms, and have good application prospects. Comprise herpes simplex virus, human papilloma virus HPV16, staphylococcus aureus, candida albicans and the like. The invention also optimizes application conditions, is suitable for developing efficient and safe vagina antibacterial gel products, and provides an innovative solution for preventing and treating gynecological infection.

The invention relates to the field of DNA (Deoxyribose Nucleic Acid) sequencing, in particular to a pathogenic microorganism high-throughput sequencing detection method and a pathogenic microorganism high-throughput sequencing detection system based on multiple PCR (Polymerase Chain Reaction) detection. The method comprises the following steps: quantitatively adding an internal reference nucleic acid marker into a first microorganism sample to be detected to generate a standardized DNA template library; performing multiple PCR amplification and quality control judgment and evaluation on the standardized DNA template library to generate a PCR quality control result table; constructing a sequencing library based on the PCR quality control result table to generate sequencing library original data; performing host reference genome comparison and removal on the original data of the sequencing library, and extracting a data set to be compared; and performing pathogenic species comparative analysis according to the to-be-compared data set, and outputting a final pathogenic microorganism detection list. The invention realizes rapid and accurate detection of pathogenic microorganisms.

The invention relates to the technical field of gene detection, and provides a fluorescent quantitative PCR (polymerase chain reaction)-based primer probe group for detecting pathogenic microorganisms of root rot of Chinese chives, which comprises forward primers JBLB-F, FPLB-F and CCLB-F, reverse primers JBLB-R, FPLB-R and CCLB-R, and probes JBLB-P, FPLB-P and CCLB-P. The invention also provides a fluorescent quantitative PCR-based leek root rot pathogenic microorganism detection kit, which comprises the primer probe group. Meanwhile, the invention also provides a fluorescent quantitative PCR-based method for detecting pathogenic microorganisms of root rot of Chinese chives. Based on a probe method fluorescent quantitative PCR technology, in combination with high-sensitivity design of a specific primer probe group, accurate detection of low-copy pathogen nucleic acid can be realized, existence and quantity of pathogens can be determined in the early stage of the pathogens of the leek root rot, and early intervention and early warning of the root rot are facilitated.

The invention relates to the technical field of biological detection, and discloses a reagent and a kit for treating a biological sample and application of the reagent and the kit. The reagent comprises a buffer solution and a nonionic surfactant, wherein the nonionic surfactant is prepared from ethyl phenyl polyethylene glycol and decyl glucoside. When the reagent provided by the invention is used for treating a biological sample, especially a biological sample with relatively high impurity content, the adverse effect of impurities on the adsorption of pathogens by magnetic beads can be effectively reduced.

The invention provides a library building method for pathogen detection, a kit and application thereof. The method comprises the following steps: S1) obtaining a first product of a nucleic acid single chain of a sample, wherein the first product is combined with a random primer; s2) performing complementary chain synthesis on the first product to obtain a second product; s3) purifying the second product to obtain a third product; s4) carrying out fragmentation treatment on the third product, connecting a joint, and directly carrying out library amplification PCR reaction to obtain a nucleic acid library of the sample; in the step (S4), a product obtained after joint connection is not purified; according to the volume fraction, the fraction ratio of a product obtained after the joint is connected in a library amplification PCR reaction system to the PCR amplification primer to the PCR amplification reagent is 4: (0.5-2): (4-5). The method can solve the problems of complex steps, long time consumption and small sample input volume of a co-construction library method in the existing NGS detection technology, and is suitable for the field of pathogenic microorganism library construction.

The present application relates to toothpaste technical field, specifically, it relates to the bacteriostatic oral care toothpaste based on active ingredient of mountain ardisia. It includes sorbitol, hydrated silicon stone, deionized water, polyethylene glycol-8, sodium lauryl sulfate, essence, polyquaternium-22, cellulose glue, sodium saccharin, hydroxypropyl guar gum, sodium methyl hydroxybenzoate and mountain ardisia extract;The bacteriostatic oral care toothpaste based on active ingredient of mountain ardisia, by introducing the mountain ardisia extract with specific chemical composition into the formula system, effectively solve the problem that the existing oral care product is not strong in specificity to periodontal pathogenic bacteria, and the bacteriostatic spectrum is relatively single;The mutual synergistic effect relationship exists between the extract, not only broaden the inhibition spectrum of periodontal pathogenic microorganism, also can reduce the oxidative stress and inflammatory response of gingival tissue, thereby maintaining oral cleaning, improve the comprehensive performance of oral care product and the mildness of use.

The application belongs to the technical field of sequencing, and particularly relates to a primer group and application thereof. The application provides a primer group, which can amplify a target library containing a normal sequencing adapter sequence through a one-step method or a two-step method. When the two-step method is used, purification of an intermediate product is not required, and the obtained amplification product has specificity and is not duplication. The primer group can be used for mNGS and / or tNGS. When the primer group is used for mNGS, the detection of a target gene can be improved. When the primer group is used for tNGS, the concentration of effective target fragments in a to-be-captured library is improved, the efficiency of pathogenic microorganism probe capture is improved, and no false positive is caused.

The utility model relates to the field of air purification, in particular to an aerosol trapping and killing integrated device which comprises a box body, an S-shaped pipe, a filter and plasma generators, the S-shaped pipe is arranged at the upper end of the box body, the filter is arranged in the box body, and two layers of plasma generators are arranged on the inner wall of the box body between the filter and the S-shaped pipe. A centrifugal fan is arranged at the lower end of the filter, a transformer is arranged in the plasma generator, a quartz medium is arranged on one side of the transformer, and a metal net is arranged on the outer wall of the quartz medium. Through the centrifugal fan, the plasma generator, the particle filter, the PLC and other components, the aerosol trapping and killing integrated device for dental diagnosis and treatment, which integrates trapping and killing functions, is developed on the basis of the composition and layout of an actual tooth diagnosis and treatment space structure; the problem that potential health hazards are caused to dental personnel due to the fact that a large number of abrasive dust suspended particles possibly carrying pathogenic microorganisms are generated in existing dental grinding diagnosis and treatment is solved.

The application provides a fluorescent rapid detection reagent and application thereof, and belongs to the technical field of bacterial detection.The fluorescent rapid detection reagent provided by the application comprises bacterial specific aptamer, priming chain and hairpin structures H1, H2 and H3.The application establishes a fluorescent specific amplification detection system with high specificity and sensitivity, develops fluorescent signal amplification labels for susceptible staphylococcus aureus and pseudomonas aeruginosa of rodents and handheld visual detection technology, and is prepared into a detection reagent, so that rapid and sensitive detection of target bacteria is realized, and the application has important significance for screening and detection of pathogenic microorganisms of experimental animals and quality control.

The application provides a tag for pathogenic microorganism detection, a library construction method and application thereof. The library construction method comprises the following steps: S1, breaking and performing end repair on DNA fragments of a pathogenic microorganism sample, and then connecting adapters to obtain adapter-connected gene fragments; and S2, purifying the adapter-connected gene fragments, and then performing PCR amplification to obtain a sample library; the adapters are connected with first tags and second tags; the first tags are nucleotides randomly composed of 6-8 bases. The application can solve the problem that it is difficult to exclude contamination in the library construction process of the pathogenic microorganism sample in the prior art, and is suitable for the field of pathogenic microorganism detection.

The application discloses a kind of biological coating with physical mechanical antibacterial and chemical antibacterial performance and preparation method thereof, it is related to biomaterial technical field.The biological coating of the application is composed of TC4 nanofiber network and nano copper deposited in nanofiber network, and the preparation method is: using polishing-washing-sweeping process pretreatment TC4 titanium alloy;It is placed in alkali solution and heated in water bath to activate, and the alloy after activation is repeated polishing, washing, sweeping step, to obtain the substrate to be sputtered;The substrate is magnetron sputtered, and nano copper is deposited on its surface;After depositing nano copper, the substrate is annealed in inert atmosphere and cooled, and the biological coating with physical mechanical antibacterial and chemical antibacterial performance is prepared.The synergistic effect of the biological coating composed of nano copper and nanofiber network can effectively kill staphylococcus aureus, escherichia coli and other pathogenic microorganisms, and can be applied to oral implant, metal implant biomaterial as functional coating.