17 results about "Photobacterium" patented technology

The invention relates to the technical field of microorganisms, in particular to an antibacterial bacillus subtilis subsp. Deserticola strain and application thereof.Bacillus subtilis subsp. Deserticola D7-HSJ9 is obtained from mangrove forest rhizosphere soil by the applicant, and it is detected that the strain has good salt resistance, alkali resistance and antibacterial characteristics, and the bacillus subtilis subsp. Deserticola D7-HSJ9 can be used as a bacillus subtilis subsp. Deserticola strain. According to the present invention, the bacterial strain has characteristics of good antibacterial effect, particularly good antibacterial effect on streptococcus iniae, and good antibacterial effect on a plurality of pathogenic bacteria such as photobacterium dama subspecies, aeromonas salmonicida, staphylococcus aureus and the like, such that the bacterial strain can effectively replace the antibiotic so as to improve the prevention and control of fish diseases, and a good germplasm resource is provided for later preparation of microecological preparation products.

The invention relates to a varied massinia, and in particular relates to a separated varied massinia P2-4 with broad-spectrum phagocytosis activity on pathogenic bacteria, and the varied massinia P2-4 is used for phagocytosis in a bacterium cracking manner, so that the activity of the varied massinia P2-4 is improved. The compound has phagocytosis activity on pathogenic bacteria such as pseudomonas aeruginosa, shewanella alginosa, aeromonas cainea, aeromonas hydrophila and photobacterium damsela; the Marsinia variabilis P2-4 provided by the invention can be applied to inhibition of pathogenic bacteria in an aquaculture water body; by adding the varied maleinia sp. P2-4 or the microbial inoculum thereof into the aquaculture feed, the immunity and / or the oxidation resistance and / or the disease infection resistance of the organism of aquaculture animals can be enhanced; the varied marssonia sp. P2-4 disclosed by the invention can be used as a feed additive for aquaculture or a biocontrol product for aquaculture, and can also be used for preventing and / or treating diseases of aquaculture animals.

The embodiment of the invention discloses application of lactobacillus delbrueckii. The application is characterized in that the lactobacillus delbrueckii is applied to inhibition of mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria. The lactobacillus delbrueckii has a preservation number of CGMCC No.11247. In the lactobacillus delbrueckii activating process, bacteriocin can be generated and can remarkably inhibit the mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria on the surfaces of fruits and vegetables, so that the preservation effect of thefruits and vegetables can be improved. Furthermore, secondary metabolites of the lactobacillus delbrueckii can be biodegraded and digested by human bodies, and are healthy and harmless to human bodies.

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 6-sialyltransferase mutant and application thereof. The a-2, 6-sialyltransferase mutants M133L, E140D, D228H, N289D and G322Q are obtained by carrying out site-specific mutagenesis on five amino acids in an a-2, 6-sialyltransferase amino acid sequence coded by a gene derived from Photobacterium sp.JT-ISH-224, and compared with wild type alpha-2, 6-sialyltransferase, the yield of the single-point mutant 6 '-SL of the E140D and the yield of the single-point mutant 6'-SL of the G322Q are increased by 87.80% and 78.01% respectively. The yield of the optimal recombinant microbial strain 6 '-SL-7 (E140D / G322Q) obtained by mutation site combination can reach 1.471 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 6'-SL.

The invention discloses bacillus velezensis Bve1910, which is preserved in the China Center for Type Culture Collection (CCTCC) and has the preservation number of CCTCC NO. M 20221965. The strain and metabolites thereof have strong antagonistic effects on more than ten types of marine culture animal pathogenic bacteria such as vibrio harveyi, vibrio erwiniae, vibrio canbayi, vibrio parahaemolyticus, vibrio alginolyticus, vibrio anguillarum, vibrio cholerae, vibrio shewanii, vibrio rotifer, pseudoalteromonas and photobacterium damarae, are strong in antibacterial activity and wide in antibacterial spectrum, and can be used for preparing marine culture animal pathogenic bacteria. According to the preparation method, the traditional Chinese medicine composition is simple in preparation method and rich and stable in metabolite, an ammonium sulfate precipitation extract, a hydrochloric acid precipitation methanol extraction extract, an ethyl acetate extraction extract and a chloroform extraction extract all have a broad-spectrum bacteriostatic effect, functional substances playing the broad-spectrum antagonistic bacteriostatic effect are compounded and diversified, and the directional selection effect on bacteria in the environment and ecology is not generated; and pathogenic bacteria are not easy to generate tolerance, so that the method has good application safety and ecological safety properties.

The present invention belongs to the technical field of bioengineering. Disclosed are Photobacterium rosenbergii and a use thereof in the preparation of a carrageenan oligosaccharide. The Photobacterium rosenbergii is specifically Photobacterium rosenbergii GDSX-4 with a 16S rDNA sequence as set forth in SEQ ID NO: 1. The Photobacterium rosenbergii GDSX-4 provided by the present invention can degrade κ-carrageenan to obtain a κ-carrageenan oligosaccharide. A crude enzyme liquid is prepared by means of the Photobacterium rosenbergii GDSX-4, and a reaction is carried out in a substrate solution containing κ-carrageenan at 40°C for 0.5-72 hours to prepare κ-neocarratetraose, κ-neocarrahexaose, and κ-neocarrabiose. The present invention provides a method for preparing a carrageenan oligosaccharide, wherein the reaction is mild and the product is uniform.

The present invention belongs to the technical field of aquatic pathogen diagnosis, and specifically relates to a multiplex fluorescence quantitative PCR detection kit for common pathogens in aquaculture, and its application and detection method. The kit contains primers and probes for detecting common pathogens in aquaculture, and the 5' end of each probe is modified with a corresponding fluorescent marker; wherein the common pathogens are Edwardsiella piscicida (Ep), Photobacterium mermanii (Pd), Vibrio rotifer (Vr) and Pseudomonas fluorescens (Pf). The method realizes the simultaneous identification and quantitative detection of Ep, Pd, Vr and Pf, has high sensitivity and good specificity, can greatly improve the detection efficiency, save the detection cost, and is suitable for large-scale rapid detection of clinical samples.

The invention provides a preparation method and application of a pomegranate rind extract, and the preparation method comprises the following steps: crushing pomegranate rind by a crusher, sieving, soaking in double distilled water, boiling with big fire, boiling with small fire for 20-60 minutes, and concentrating with big fire to obtain the pomegranate rind extract. Compared with the prior art, the pomegranate peel single Chinese herbal medicine aqueous extract prepared by the invention has a remarkable inhibition effect on apostichopus japonicus skin ulcer pathogenic bacteria photobacterium damsonii, is relatively low in toxicity, and can be used for preventing and controlling apostichopus japonicus skin ulcer syndrome caused by the photobacterium damsonii. Moreover, a proper amount of pomegranate peel aqueous extract is mixed with bait to feed the apostichopus japonicus, so that the capability of resisting photobacterium damsonii subspecies infection of the apostichopus japonicus can be remarkably enhanced, and the survival rate of the apostichopus japonicus is increased.

The invention provides a genetically engineered bacterium as well as a preparation method and application thereof. The genetically engineered bacterium contains an orfZ gene which is introduced from an external source; the genetically engineered bacterium is selected from photobacterium LN01. When the genetically engineered bacterium is fermented by taking cane sugar and gamma-butyrolactone as precursors, the P3HB4HB can be efficiently synthesized, and the content of 4HB in the P3HB4HB is high. The genetically engineered bacterium is expected to be applied to industrial production of bio-based plastics, efficient production of P3HB4HB with high content of 4HB is realized, and the genetically engineered bacterium is of great significance to promotion of wide application of biopolyester and reduction of negative effects of petroleum-based plastics on the environment.

The invention relates to a culture medium for improving the luminescent property of luminescent bacteria. The culture medium comprises 5.43-26.48 w / v% of a carbon source; 2.02 to 16.16 w / v% of a nitrogen source; 0.53 to 2.64 w / v% of potassium ions; 0.0047 to 1.89 w / v% of sodium ions; 0.024 to 1.46 w / v% of magnesium ions; 0.0028 to 23.43 w / v% of chloride ions; 0.87 to 4.35 w / v% of phosphate ions; and 0.064 to 3.83 w / v% of sulfate ions. The improved culture medium effectively enhances the luminous property of marine luminous bacteria, and has wide application prospects in the fields of marine ecological research, environmental monitoring and the like.

The invention relates to the technical field of wastewater detection, and discloses a wastewater biotoxicity detection method which comprises the following steps: (1) adding an organic solvent into to-be-detected wastewater, centrifuging, and taking supernate; (2) dispersing the freeze-dried photogenic bacteria in a 2% sodium chloride solution, and resuscitating at normal temperature to obtain resuscitated bacteria liquid with activity; (3) diluting the to-be-tested wastewater according to a preset dilution ratio, and then carrying out a luminous bacterium method biotoxicity test to obtain a light inhibition rate gamma value; and (4) making a double logarithmic line graph by using the light suppression rate gamma value and the dilution ratio, and calculating to obtain an EC50 value through an interpolation method. According to the present invention, the biological toxicity test is performed by using the luminous bacteria method, the biological toxicity condition of the to-be-detected wastewater can be rapidly determined, and the dimethyl sulfoxide is added to the to-be-detected wastewater to assist the dissolution of the indissolvable organic matter before the detection, such that the solubility of the organic matter in the to-be-detected wastewater can be effectively improved, and the accuracy of the determination result can be improved.

The utility model discloses a self-luminous bacterium display and observation device which comprises a box body, a display area is arranged on the side wall of the box body, the side wall, corresponding to the display area, of the box body is a light-transmitting display surface side wall, and the display surface side wall is detachably connected with the box body; a background block is arranged in the box body, a concave image-text groove with patterns or characters is carved in the surface, facing the side wall of the display face, of the background block, and the background block is attached to the side wall of the display face, so that the space between the concave image-text groove and the side wall of the display face becomes a closed self-luminous bacterium liquid containing channel. The utility model further discloses another self-luminous bacterium display and observation device comprising the image-text box body. The self-luminous bacterium display device is used for teaching, science popularization and landscape, and is reasonable in structural design, easy to produce and manufacture, low in cost, convenient to mount and dismount, attractive in appearance and capable of achieving interesting teaching.

The invention belongs to the technical field of marine organisms, and particularly relates to application of a gene cluster in detection of photobacterium damsela and a detection primer probe set. The primer probe group is a complete set of primer combination corresponding to three gene loci, and consists of upstream and downstream primers and probe primers; according to the invention, corresponding RPA (recombinase polymerase amplification) detection primers are designed according to three specific gene loci obtained by screening aiming at the existing known photobacterium damsela, and a test strip RPA detection system and method of the photobacterium damsela are established; the technology has an important value for on-site rapid screening and detection of the photobacterium damsonii involved in aquaculture, import and export trade and bathing beaches, and has a wide market application prospect.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus isothermophilus and application thereof in inhibition of edwardsiella ictaluri and photobacterium damsonii. The invention relates to a strain of bacillus straticola, which is a strain K of bacillus straticola, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 19, 2025, and the preservation number of the strain is CGMCC No.33567. The invention further discloses a preparation method of the bacillus straticola. The strain can be used as a feed additive for preventing and treating edwardsiella ictaluri and photobacterium damsonii in aquaculture, or can be directly prepared into a feed, and the antibacterial effect is remarkable when the bacterial feed is fed in the aquaculture, or the strain can be added into a water body to be used as a water body disinfectant.

The invention relates to the technical field of biology, and discloses a multiplex PCR (Polymerase Chain Reaction) detection primer combination, a kit and a method for simultaneously detecting multiple marine pathogenic bacteria. Specific primer pairs are respectively designed for marine pathogenic bacteria such as vibrio alginolyticus, vibrio vulnificus, photobacterium dama subspecies, vibrio harveyi and vibrio candersonii, a freeze-drying technology is combined, and the amplification efficiency and specificity are improved through primer design and optimization of an amplification system and detection program parameters. In addition, the method has relatively high accuracy, and shows an efficiency advantage, a relatively short detection period and a convenient result judgment characteristic when being used for detecting various marine pathogenic bacteria. Aiming at the field of detection of diseases caused by various marine pathogenic bacteria, the invention provides a reliable detection way, which is of great significance in practical application scenes such as aquaculture disease monitoring, prevention and control, marine organism health assessment and the like, and is helpful for promoting development and perfection of related disease detection technologies.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to a photobacterium strain and application thereof in straw degradation. The invention discloses a photobacterium strain Photobacterium sedimentimangrovi GSS17 which is preserved in Guangdong Microbial Culture Collection Center on January 11, 2023, and the preservation number of the photobacterium strain Photobacterium sedimentimangrovi GSS17 is GDMCC (China General Microbiological Culture Collection Center) No. 62977. Compared with the prior art, the strain can secrete various active enzymes related to carbohydrate degradation and can accelerate rapid conversion of cellulose and hemicellulose substances so as to promote straw degradation.

The invention provides a method for detecting the mermaid luminescent bacillus mermaid subspecies, comprising extracting DNA from a single colony of the mermaid luminescent bacillus mermaid subspecies or extracting DNA from a fish tissue to be detected, and designing specific primers based on the subspecies level, taking the DNA of the sample to be detected as a template, performing PCR amplification detection through an upstream primer P1 and a downstream primer P2 pair, and performing electrophoresis analysis. The invention can accurately distinguish the mermaid luminescent bacillus mermaid subspecies and the piscivorous subspecies in clinical practice, and has high application value in aquaculture.