5 results about "Photobacterium" patented technology

The embodiment of the invention discloses application of lactobacillus delbrueckii. The application is characterized in that the lactobacillus delbrueckii is applied to inhibition of mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria. The lactobacillus delbrueckii has a preservation number of CGMCC No.11247. In the lactobacillus delbrueckii activating process, bacteriocin can be generated and can remarkably inhibit the mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria on the surfaces of fruits and vegetables, so that the preservation effect of thefruits and vegetables can be improved. Furthermore, secondary metabolites of the lactobacillus delbrueckii can be biodegraded and digested by human bodies, and are healthy and harmless to human bodies.

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 6-sialyltransferase mutant and application thereof. The a-2, 6-sialyltransferase mutants M133L, E140D, D228H, N289D and G322Q are obtained by carrying out site-specific mutagenesis on five amino acids in an a-2, 6-sialyltransferase amino acid sequence coded by a gene derived from Photobacterium sp.JT-ISH-224, and compared with wild type alpha-2, 6-sialyltransferase, the yield of the single-point mutant 6 '-SL of the E140D and the yield of the single-point mutant 6'-SL of the G322Q are increased by 87.80% and 78.01% respectively. The yield of the optimal recombinant microbial strain 6 '-SL-7 (E140D / G322Q) obtained by mutation site combination can reach 1.471 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 6'-SL.

The invention provides a genetically engineered bacterium as well as a preparation method and application thereof. The genetically engineered bacterium contains an orfZ gene which is introduced from an external source; the genetically engineered bacterium is selected from photobacterium LN01. When the genetically engineered bacterium is fermented by taking cane sugar and gamma-butyrolactone as precursors, the P3HB4HB can be efficiently synthesized, and the content of 4HB in the P3HB4HB is high. The genetically engineered bacterium is expected to be applied to industrial production of bio-based plastics, efficient production of P3HB4HB with high content of 4HB is realized, and the genetically engineered bacterium is of great significance to promotion of wide application of biopolyester and reduction of negative effects of petroleum-based plastics on the environment.

The invention relates to a culture medium for improving the luminescent property of luminescent bacteria. The culture medium comprises 5.43-26.48 w / v% of a carbon source; 2.02 to 16.16 w / v% of a nitrogen source; 0.53 to 2.64 w / v% of potassium ions; 0.0047 to 1.89 w / v% of sodium ions; 0.024 to 1.46 w / v% of magnesium ions; 0.0028 to 23.43 w / v% of chloride ions; 0.87 to 4.35 w / v% of phosphate ions; and 0.064 to 3.83 w / v% of sulfate ions. The improved culture medium effectively enhances the luminous property of marine luminous bacteria, and has wide application prospects in the fields of marine ecological research, environmental monitoring and the like.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to a photobacterium strain and application thereof in straw degradation. The invention discloses a photobacterium strain Photobacterium sedimentimangrovi GSS17 which is preserved in Guangdong Microbial Culture Collection Center on January 11, 2023, and the preservation number of the photobacterium strain Photobacterium sedimentimangrovi GSS17 is GDMCC (China General Microbiological Culture Collection Center) No. 62977. Compared with the prior art, the strain can secrete various active enzymes related to carbohydrate degradation and can accelerate rapid conversion of cellulose and hemicellulose substances so as to promote straw degradation.