2 results about "Apostichopus japonicus" patented technology

This invention belongs to the field of marker-assisted breeding technology, and relates to the combination and application of SNP molecular markers for intestinal regeneration in *S. spinosaurus*. This invention uses the ratio of open intestinal length to total intestinal length during intestinal regeneration as a continuous trait indicator of intestinal regeneration capacity. Using whole-genome resequencing data and the BLINK model, genome-wide association analysis was performed to screen for 22 SNP molecular markers significantly associated with intestinal regeneration capacity in *S. spinosaurus*. The nucleotide sequences corresponding to these SNP molecular markers are shown in SEQ ID NO. 1-22, each sequence being 200 bp in length, with the SNP site located at the 100th bp. By detecting the genotype of these SNP sites, the intestinal regeneration capacity of *S. spinosaurus* can be evaluated at the molecular level, and superior individuals with faster intestinal regeneration can be screened for use in stress-resistant germplasm screening, marker-assisted breeding, and the construction of high-regeneration-capacity *S. spinosaurus* breeding populations.

The application relates to the technical field of sea cucumber collagen, in particular to a protein with a repairing effect and a preparation method and application thereof. The amino acid sequence of the protein is from NCBI database collagen [Apostichopus japonicus] GenBank: APA22677.1, is optimized through codons, is further expressed and screened through a shake flask and a 10L fermentation tank, and finally a Pichia sp. strain with a high protein expression amount is found, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 18, 2025, and has a preservation number of CGMCC No. 34894. The obtained pure protein has excellent repairing effect and certain antioxidant effect, and has great commercial value.