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Anti-oxidation and DPP-IV inhibition active peptide derived from apostichopus japonicus

A DPP-IV, inhibitory activity technology, applied in the field of marine biological small molecule active peptides, can solve the problem of no DPP-IV inhibitory active peptide, etc., achieve strong DPP-IV inhibitory activity, strong antioxidant activity, separation and purification steps simple effect

Active Publication Date: 2019-03-01
DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, regarding the separation of active peptides in sea cucumbers, there are only patent reports on the separation of two antioxidant active peptides (NO: CN 104402972A, CN 104356201 A), but there is no report on DPP-IV inhibitory active peptides

Method used

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  • Anti-oxidation and DPP-IV inhibition active peptide derived from apostichopus japonicus
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  • Anti-oxidation and DPP-IV inhibition active peptide derived from apostichopus japonicus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] An anti-oxidation and DPP-IV inhibitory active peptide derived from sea cucumber, the amino acid sequence of the active peptide is Ser-Arg-Pro-Gln-Tyr-Pro-Gln-Tyr-Pro-Ser.

[0035] With the active peptide sequence as the core, any corresponding adjustments or modifications are made to it.

[0036] The active peptide has anti-oxidation and DPP-IV inhibitory activities, and can be applied to the preparation of hypoglycemic or anti-oxidation food, health products and medicines.

Embodiment 2

[0038] Such as figure 1 As shown, the method for separating and purifying the anti-oxidation and DPP-IV inhibitory activity peptides of A. japonicus origin described in Example 1 comprises the following steps:

[0039] S1. Preparation of enzymatic hydrolyzate of Apostichopus japonicus

[0040] Add 2 times the mass volume of water to the fresh sea cucumber body wall to make a homogenate and place it in an enzymolysis tank, then add 2‰ of the sea cucumber quality compound protease, the mass ratio of the compound protease is: neutral protease: papain: Flavor protease = 2:3:3, enzymatic hydrolysis at 40°C for 4 hours, the pH value of the enzyme reaction was controlled at 8.0, and after the enzymolysis was completed, the temperature was raised to 90°C to inactivate the enzyme for 10 minutes to obtain the sea cucumber protein enzymatic hydrolyzate.

[0041] S2. Ultrafiltration membrane separation of Apostichopus japonicus protein enzymatic hydrolyzate

[0042] The sea cucumber pro...

Embodiment 3

[0061] The anti-oxidation of sea cucumber source described in this embodiment and each step of the separation and purification method of DPP-IV inhibitory active peptide are all the same as in Example 2, the difference is:

[0062] (1) In step S1, add 2.5 times the mass volume of water to the body wall of fresh sea cucumber to make a homogenate and place it in an enzymolysis tank, then add 3.5‰ of the mass of sea cucumber to the compound protease. The mass ratio of the compound protease is: Sexual protease: papain: flavor protease=3:4:4, enzymolysis at 45°C for 4 hours, and the pH value of the enzyme reaction is controlled at 8.5;

[0063] (2) In step S3, the mobile phase is 30% methanol, and the flow rate is 0.4 mL / min;

[0064] (3) Mobile phase A in step S4 is 0.75% trifluoroacetic acid water (V / V);

[0065] (4) The detection wavelength in step S4 is 220 nm.

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Abstract

The invention belongs to the field of marine organism small-molecular active peptides, and particularly relates to an anti-oxidation and DPP-IV inhibition active peptide derived from apostichopus japonicus by enzymolysis. The amino acid sequence of the anti-oxidation and DPP-IV inhibition active peptide is Ser-Arg-Pro-Gln-Tyr-Pro-Gln-Tyr-Pro-Ser. The anti-oxidation and DPP-IV inhibition active peptide is prepared by the following steps: adding water into fresh apostichopus japonicus for homogenizing, and placing the homogenate in an enzymolysis tank; performing enzymolysis with compound protease to obtain enzymatic hydrolysate; treating the enzymatic hydrolysate with a membrane separation technology to obtain a small-molecular active peptide crude product; separating the crude product through Sephadex LH-20 to obtain a small-molecular active peptide. The purity of the small-molecular active peptide as measured by adopting RP-HPLC (Reversed-Phase High Performance Liquid Chromatography)is greater than 95 percent, and the amino acid composition of the active peptide is measured by high performance liquid chromatography / mass spectrometry, so that the amino acid sequence of the small-molecular peptide is determined finally. The active small-molecular peptide derived from the apostichopus japonicus has the advantages of small molecular weight, simple separating-purifying steps, easiness in preparation, high purity and higher oxidization resistance and DPP-IV inhibition activity, has the characteristics of naturalness, safety and high efficiency, can be applied to the preventionand treatment of relevant diseases such as diabetes mellitus as an antioxidant as well as a DPP-IV inhibitor, and has a wide application prospect in the fields of foods, health care products and medicines.

Description

technical field [0001] The invention belongs to the field of small molecule active peptides of marine organisms, and in particular relates to anti-oxidation and DPP-IV inhibitory active peptides derived from sea cucumber enzymolysis. Background technique [0002] Peptide drugs have the characteristics of high biological activity, strong specificity, weak immunogenicity, and good stability, making peptide drugs the focus of new drug research and development. More and more peptide drugs have been developed and used in clinical practice. Currently, peptide drugs It is mainly derived from endogenous polypeptides or some natural polypeptides, but these endogenous or natural polypeptides have problems such as low content in organisms, difficulty in extraction, and difficulty in obtaining. With the continuous advancement of bio-enzyme technology, the activity of enzymatic sources Polypeptides become an effective way to obtain active polypeptides. [0003] The enzymatic active pept...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/16C07K1/14C12P21/06
CPCC07K7/06C12P21/06
Inventor 包卫洋马普孙天利左爱华王祖哲
Owner DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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