162 results about "Sephadex" patented technology

The invention belongs to the field of egg by-product processing, and particularly relates to a preparation method and application of eggshell membrane peptides, the preparation method of the eggshell membrane peptides comprises the following steps: S1, drying and crushing cleaned eggshells, and then separating eggshell membranes; s2, putting the eggshell membrane into a steam explosion machine, carrying out pressure maintaining treatment for 1-5 minutes under the pressure of 1.0-1.5 MPa, then instantly releasing the pressure, and collecting a steam explosion sample; s3, adding the eggshell membrane subjected to steam explosion treatment into a sodium hydroxide aqueous solution with the pH value of 7-9 according to the solid-liquid ratio of 1: (20-30), then adding alkaline protease according to the enzyme addition amount of 5000-15000U / g, carrying out enzymolysis for 5-10 hours at the temperature of 35-65 DEG C, then inactivating for 3-5 minutes at the temperature of 100 DEG C, filtering enzymatic hydrolysate, taking filtrate, centrifuging, taking supernate, and freeze-drying to obtain polypeptide; s4, polypeptide obtained in S3 is dissolved in an activation solution to be activated for 8-10 h at the temperature of 4-8 DEG C; and S5, separating the sample activated in the step S4 through a Sephadex G-15 chromatographic column to obtain the activated eggshell membrane peptide. Based on the method, the preparation efficiency is high, and the prepared eggshell membrane peptide is high in antioxidant activity.

The invention belongs to the field of natural product extraction, and relates to a method for extracting and separating flavonoid compounds from Artemisia chrysantha, which comprises the following steps: carrying out reflux extraction on the dry overground part of Artemisia chrysantha by using 70% ethanol, loading to a balanced D101 macroporous adsorption resin column, sequentially eluting by using water, a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution, and collecting the eluent. Four fractions from Fr. I to Fr. IV are obtained; respectively carrying out polyamide column chromatography separation on Fr.II to Fr.IV, and sequentially eluting with a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution to respectively obtain three fractions; and further purifying the Fr.II-1, the Fr.II-2, the Fr.III-1, the Fr.III-2, the Fr.III-1 and the Fr.III-3 by virtue of a Sephadex LH-20 column, so as to finally obtain seven flavonoid compounds. According to the method, only the ethanol water low-toxicity solvent is used for extraction and preparation, organic solvents with high toxicity are not used, the method is green and environment-friendly, the extraction efficiency is high, the number of extracted compounds is large, chemical components of the artemisia europaea medicinal material are enriched, the resource source of active components is widened, and a foundation is laid for follow-up research.

The invention discloses a method for preparing various bioactive peptides based on bean dreg fermentation, which comprises polypeptides with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and belongs to the field of microbial biological fermentation. Bacillus amyloliquefaciens sourced from fermented soybeans is used for fermentation, and a mixed product with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity is obtained. The invention also provides a method for purifying the polypeptide with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, which is characterized in that the mixed product is purified by combined column chromatography, the combined column chromatography comprises cation exchange resin LXP-01, macroporous adsorption resin HP20, weak base anion exchange resin D941, silica gel resin and Sephadex LH-20. The invention also discloses seven bioactive peptides with biological activity, the amino acid sequences are CKLLL, APGYSC, FPKYG, FPGPLV, FPRSH, FMAV and SVCC, and the bioactive peptides have good alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and can be used for producing functional foods, health care products, cosmetics and the like for assisting in reducing blood sugar, reducing blood pressure, resisting oxidation and the like.

The invention discloses a novel benzofuran component in eupatorium chinense and application of the benzofuran component in anti-inflammatory activity. The obtained compound is separated from an eupatorium chinense extract. The eupatorium chinense root extract is separated by using an extraction method, macroporous adsorption resin impurity removal, Sephadex LH-20 gel column chromatography, high performance liquid chromatography and other methods. According to the present invention, all the separated compounds and the analogues thereof have good inhibition activity on target cyclooxygenase-2 of inflammation-related diseases, and have good inhibition activity on COX-2 enzyme, and the molecular docking experiment results show that the compounds and the target proteins have good binding energy. Therefore, the compound can be used for preparing drugs for treating inflammatory related diseases, or anti-inflammatory and analgesic drugs, or tumor drugs by inhibiting COX-2 enzyme, such as drugs for rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, Alzheimer's disease and the like, and related natural lead compounds are provided.

The invention relates to a method for separating and purifying polygonatum sibiricum oligosaccharide. According to the method, the polygonatum sibiricum polysaccharide and the filler or / and the filter aid are fully mixed and then separated and purified; wherein the filler is prepared from Sephadex G-15 (crosslinked glucose gel), DEAE-Sepharose CL-4B (agar gel) and Bio-Gel P-2 (polyacrylamide gel), the filter aid is prepared from kieselguhr, perlite, cellulose, activated carbon and red soil, and the method has the advantages that the Mwlt can be obviously improved; the yield of the 2KDa polygonatum sibiricum oligosaccharide is increased, and the effect of combined use of the filler and the filter aid is optimal. The addition of the filter aid can avoid the blockage of filler pores in the middle and later stages of elution, improve the elution performance of the filler on polysaccharide, and accelerate the elution and purification speed of the polygonatum kingianum oligosaccharide. Experiments show that the oligosaccharide can significantly enhance the proliferative activity and phagocytosis of macrophages, promote RAW264.7 cells to secrete TNF-alpha, IL-6, NO and other cell factors, and improve the cellular immunocompetence. The method has the characteristics of convenience, high efficiency and low cost, and has relatively high application value.

The present invention discloses a preparation method of Inonotus hispidus polysaccharide IHP-1aa: It is obtained by removing fat with ethanol, extracting with water and precipitating with ethanol, then removing proteins by the Sevage method, separating neutral polysaccharide components through a DEAE-52 cellulose column and a DEAE agarose gel column, and finally purifying with Sephadex G-100. The Inonotus hispidus polysaccharide has uniform purity and clear composition, and is a heteropolysaccharide composed of glucose, galactose, mannose, fucose and methylgalactose, with a molecular weight of 26.9 kDa. In vitro experiments show that it has no adverse effects on normal cells, can significantly stimulate macrophages RAW264.7 to secrete NO, IL-6 and TNF-α, has good immunomodulatory activity, and can be used for the development of immunomodulatory products.

The invention relates to the technical field of polysaccharide preparation, in particular to mesona chinensis acidic polysaccharide and an extraction method thereof. The extraction method comprises the following steps: 1) crushing, degreasing and drying dried mesona chinensis, boiling and performing alkali extraction to obtain a mesona chinensis extracting solution; macroporous resin D-900 is adopted for adsorption to remove pigments, and crude mesona chinensis benth polysaccharide is obtained; carrying out primary purification by adopting DEAE-52 ion exchange column chromatography; and performing chromatographic purification through a Sephadex G-100 ion exchange column, collecting components, and performing freeze-drying to obtain the mesona chinensis benth acidic polysaccharide. The extraction method of the mesona chinensis benth acidic polysaccharide has the beneficial effects that the operation is simple, the cost is low, the characteristics of good decoloring effect and high polysaccharide preservation rate are also realized, and the biological activity of the mesona chinensis benth acidic polysaccharide can be guaranteed. Compared with other resins, the D-900 macroporous resin is used for selectively adsorbing melanin, the adsorption capacity of the D-900 macroporous resin to mesona melanin is remarkably higher than that of other resins, the adsorption rate of the D-900 macroporous resin to polysaccharide is lower than 2%, and the D-900 macroporous resin has the advantages of being good in decolorization effect and high in polysaccharide preservation rate.

The invention belongs to the technical field of biology, and discloses a bioactive peptide which is a micromolecular anti-tumor bioactive peptide derived from sea cucumber, and the amino acid sequence of the bioactive peptide is FNPDTFD. Sephadex G-15 gel chromatography and mass spectrometry technologies are used for separating, purifying and structurally identifying the sea cucumber flower enzymolysis peptide, so that the small molecule peptide with a potential anti-tumor effect is obtained. A cell viability experiment verifies that the small molecule peptide can selectively inhibit the proliferation of EGFR mutant non-small cell lung cancer cells. Besides, molecular docking and protein immunoblotting results show that the peptide can be combined with EGFR mutant protein, and the activation of a downstream Akt / mTOR signal channel is inhibited by targeting the EGFR mutant protein, so that the biological activity of resisting the non-small cell lung cancer is exerted. The bioactive peptide not only has the potential of being developed into a new generation of EGFR targeting antitumor drugs, but also has wide application prospects in the field of functional health food. The preparation process is simple, and is suitable for industrial production and market popularization and application.

The invention discloses a novel flavonoid compound as well as a preparation method and application thereof, and belongs to the technical field of natural medicines. The preparation method comprises the following steps: heating, refluxing and extracting with ethanol, and concentrating into thick extract under reduced pressure to obtain a crude extract; step 2, extraction; step 3, loading the dichloromethane extract on an MCI column; 4, concentrating the 30% methanol eluent to prepare a liquid phase; and step five, purifying the compound crude product (I) and the compound crude product (II) through a Sephadex LH-20 gel column respectively, and eluting with methanol to obtain the compound (I) and the compound (II) respectively. The anti-gastric cancer drug provided by the invention comprises two new flavonoid compounds in a specific mass ratio, and the composition has a more excellent anti-gastric cancer effect. According to the invention, two new flavonoid compounds are separated from the pogonatum insignis plant, and the two new compounds have good anti-gastric cancer activity, so that a foundation is laid for development and utilization of pogonatum insignis plant resources.

The invention relates to a preparation method and application of a sea cucumber egg-derived peptide compound. The preparation method of the sea cucumber egg source peptide compound comprises the following steps: performing synchronous enzymolysis on sea cucumber egg powder by using alkaline protease-trypsin; the sea cucumber ovum source active peptide is obtained by further carrying out ultrafiltration, sephadex chromatography and high performance liquid chromatography separation and adopting liquid chromatography-mass spectrometry technology analysis, a computer-aided screening technology and in-vitro verification, and the amino acid sequence of the sea cucumber ovum source active peptide comprises one or more of WGN, WI GG and GFPVG (Fibroblast Growth Factor Peptide Growth Growth Growth Growth Growth Growth Growth Growth Growth Growth Growth Growth Growth Growth Growth Growth Growth Growth). The sea cucumber ovum-derived peptide compound and the sea cucumber ovum-derived active peptide provided by the invention have double activities of resisting oxidation and inhibiting tyrosinase, can reduce the melanin content, and have the advantages of good water solubility, good safety and the like.

The invention relates to the technical field of biological medicines, in particular to a polycyclic polyisopentenyl cyclopentanone compound as well as a preparation method and application thereof. The polycyclic polyisopentenyl cyclopentanone compound provided by the invention has a structural formula as shown in a formula 1; the preparation method comprises the following steps: by taking dry fruits of hypericum berry as raw materials, carrying out percolation extraction and concentration to obtain an extract, suspending the extract in water to obtain a suspension, and extracting and concentrating the suspension with petroleum ether to obtain a crude extract; the crude extract is taken and subjected to positive and negative phase silica gel column separation, MCI medium-pressure column chromatography, Sephadex LH-20 gel column chromatographic separation, high performance liquid chromatography preparation and CHIRALPAK IG chiral column resolution, and the polycyclic polyisopentenyl cyclopentanone compound is obtained. The protection activity of the compound on AC16 myocardial cell injury induced by cold ischemia is evaluated, and the compound can be used for preparing the polycyclic polyisopentenyl cyclopentanone compound. The compounds can be used as lead compounds for developing medicines for preventing and treating myocardial cold ischemia injury.

Hippophae rhamnoides peel polysaccharides, and a preparation method therefor and a use thereof. The hippophae rhamnoides peel polysaccharides comprise the following monosaccharides: rhamnose, mannose, galactose, glucose, galacturonic acid, and arabinose. The preparation method comprises: degreasing, extracting, concentrating and performing alcohol precipitation to obtain water-soluble crude polysaccharides; and then using a non-polar macroporous adsorption resin to perform purification to obtain hippophae rhamnoides peel polysaccharides. The yield and content of the polysaccharides are high. By using the non-polar microporous adsorption resin to replace a relatively costly DEAE-cellulose and Sephadex gel, active polysaccharides in the Hippophae rhamnoides peel are enriched, the yield and purity of the polysaccharides are also greatly improved, and the polysaccharides have low production costs and are used for industrial batch production. The hippophae rhamnoides peel polysaccharides have significant effects of resisting ulcerative colitis, and alleviating constipation, indigestion, and intestinal flora disorder.

The present invention belongs to the field of agarwood, and in particular to a kind of 2-(2-phenylethyl) chromone and lignan heterocompound and its application. The present invention uses the agarwood produced by thick-leaved agarwood tree as raw material, and after reflux extraction, successively adopts silica gel, Sephadex LH-20 gel, high performance liquid chromatography and other chromatographic techniques to separate and purify to obtain 1 monomer compound, and its structure is identified by spectral methods such as mass spectrometry and nuclear magnetic resonance and combined with physicochemical properties. It is retrieved as a new compound by SciFinder and named as thick-leaved agarwood alcohol (aquicrassnol), which is the first heterocompound formed by 2-(2-phenylethyl) chromone and lignan found in agarwood. It has been verified by experiment that the compound has an inhibitory effect on the production of nitric oxide in mouse mononuclear macrophage RAW264.7 induced by lipopolysaccharide, and its half-maximal inhibitory concentration value is 47.53±2.14μmol / L, which has anti-inflammatory activity.

The invention relates to the technical field of natural medicines, in particular to a 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound as well as a preparation method and application thereof. The 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound disclosed by the invention has a structure as shown in a formula I; the method comprises the following steps: extracting hypericum monogynum by using ethanol and petroleum ether, sequentially eluting the obtained petroleum ether extract through silica gel column chromatography, MCI resin column chromatography, Sephadex LH-20 gel column chromatography and ODS medium-pressure column chromatography, and purifying the collected eluate through preparative ODS liquid chromatography and semi-preparative ODS liquid chromatography to obtain the 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound. The 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound is extracted from hypericum monogynum for the first time, and the compound has great development value as a novel medicine for preventing or treating calcified aortic valve diseases.

The invention relates to a medicine for promoting skin injury repair and healing and a preparation method thereof, and belongs to the technical field of biological medicines.The medicine is prepared from a radix astragali preparata extract, lipidosome encapsulated panax notoginseng total saponin-RGD peptide, a honeysuckle flower-maca extract, an asiaticoside-hydroxypropyl-beta-cyclodextrin inclusion compound and dipotassium glycyrrhizinate; the radix astragali preparata extract is a product obtained by moistening radix astragali with honey, baking, extracting with deionized water, and adsorbing and purifying with a Sephadex G-15 column; the honeysuckle-maca extract is a product obtained by mixing dry honeysuckle powder and dry maca root powder according to the mass ratio of (3-5): (1-1.7), performing enzymolysis through cellulase, then performing common enzymolysis through ficin protease and papain, and performing adsorption and purification through an HPD722 macroporous adsorption resin column. All the components are compatible according to a specific proportion, so that the allergy and skin irritation risks are reduced, skin injury healing is reasonably promoted, and melanin deposition is effectively inhibited.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

The application provides a momordica root polysaccharide and a preparation method and application thereof, and belongs to the technical field of natural product development. The preparation method of the momordica root polysaccharide comprises the following steps: drying and crushing momordica roots, and removing fat by ethanol reflux; after water extraction and alcohol precipitation, and removing protein, a momordica root crude polysaccharide is obtained; and the momordica root crude polysaccharide is sequentially separated and purified by DEAE-52 cellulose column and Sephadex LH20 gel column to obtain the momordica root refined polysaccharide. The preparation method has the advantages that the polysaccharide obtained by directly extracting water in the prior art has a narrow molecular weight distribution range and better immune enhancement activity. Experimental results show that the momordica root polysaccharide can stimulate RAW264.7 cells to secrete NO, TNF-alpha and IL-6 factors, enhance the phagocytosis of RAW264.7 cells, and thus enhance the cell immune function, and can be applied to the development of products for improving immunity.

The invention provides a traditional Chinese medicine composition for reducing blood sugar and blood pressure and a preparation method thereof, and belongs to the technical field of biological medicine, the composition comprises ivory white eggplant extract, parsley extract, banana zucchini rattan extract, coloured bean puffball extract, eucommia ulmoides glycoside and puerarin. The preparation method comprises the following steps: carrying out common enzymolysis on ivory white eggplant by using cellulase, pectinase and papain, and purifying by using an XAD-16 macroporous resin column and a Sephadex LH-20 gel column to obtain an ivory white eggplant extract; the method comprises the following steps: extracting parsley with an ethanol aqueous solution, and purifying with an AB-8 macroporous adsorption resin column to obtain a parsley extract; the preparation method comprises the following steps: extracting banana zucchini rattan by using an ethanol water solution, and purifying by using a D101 macroporous adsorption resin column to obtain a banana zucchini rattan extract; colored bean puffball is subjected to common enzymolysis with chitinase and ficin, extracted with a two-phase solvent and purified with an HPD-100 macroporous adsorption resin column, and the colored bean puffball extract is prepared. All the components are matched to realize synergy of reducing blood sugar and blood pressure.

The present disclosure provides use of an effective part extract of Monochasma savatieri in preparation of a drug for treating an inflammatory disease or a tumor. In the present disclosure, an effective part of Monochasma savatieri is separated and purified by various separation and purification methods (including solvent extraction, macroporous resin column chromatography, MCI column separation, and Sephadex LH-20 gel column separation) separately, to obtain the effective part extract of Monochasma savatieri. The effective part extract of Monochasma savatieri is capable of significantly inhibiting secretion of TNF-α, and significantly inhibiting proliferation of breast cancer cells and lung cancer cells, thereby effectively treating the inflammatory disease or the tumor. The present disclosure provides a favorable theoretical basis for finding a drug that can effectively treat the inflammatory disease or the tumor from the Monochasma savatieri, which is of great significance to development of novel drugs.

The present invention discloses a preparation process of glycyrrhiza polysaccharide capable of promoting T cell proliferation. The glycyrrhiza polysaccharide capable of promoting T cell proliferation can be obtained by extraction of glycyrrhiza polysaccharide, purification with activated carbon and modified adsorption resin, and then further removal of impurities by using DEAE-52 cellulose column for anion exchange, a membrane comprised of hollow fibers and Sephadex G-100 for adsorption. Compared with the prior art, the preparation process of glycyrrhiza polysaccharide of the present invention has the advantages that impurities such as pigments, proteins and the like in the glycyrrhiza polysaccharide can be removed, the content and the activity of glycyrrhiza polysaccharide are retained, the purity of the glycyrrhiza polysaccharide is improved, and the obtained glycyrrhiza polysaccharide has the effect of promoting T cell proliferation.

The invention discloses neutral refined polysaccharide of longan aril from Guangxi, and belongs to the technical field of bioengineering. The invention particularly relates to a preparation and characterization method and structural analysis of the high-purity longan pulp neutral refined polysaccharide LYP-1-s. The polysaccharide is separated and purified from longan pulp through a water extraction and alcohol precipitation method in combination with DEAE-FF and Sephadex G-75 column chromatography, the yield is 4.81 + / -0.06%, and the purity is high. TLC and ultraviolet full spectrum analysis results show that LYP-1-s is high in component enrichment degree, few in impurities, high in activity and uniform in molecular weight distribution. The polysaccharide is neutral polysaccharide, is easily soluble in water and is insoluble in organic solvents such as ethanol. In the digestion process, stable antioxidant activity can be kept, and certain life benefiting activity is shown on intestinal flora. Therefore, LYP-1-s has application potential as a novel health food raw material, and can be used for development of related industries.

The invention relates to the technical field of traditional Chinese medicine extraction and separation, in particular to two alkaloid compounds in portulaca oleracea and an extraction and separation method and application thereof. The molecular formulas of the two kinds of alkaloid compounds are both C27H33NO15, and the two kinds of alkaloid compounds are respectively named as oleraclamie K and oleraclamie L. The invention further discloses a preparation method of the oleraclamie. According to the method, water extraction, ODS column chromatography, Sephadex LH-20, a high performance liquid chromatograph and the like are adopted for separation, purification and preparation, the compounds oleraclamie K and oleraclamie L are successfully extracted and separated out, the method is simple, convenient and rapid, the extraction and separation process mainly adopts a water extraction process, the method is environmentally friendly, the purity of the compounds separated through the method is high and larger than 90%, and the method is suitable for industrial production. In addition, researches show that the two compounds have anti-inflammatory effects, so that the alkaloid compound and the salt and the derivative thereof can be used as synthesis primers of other compounds and raw materials for new drug development and pharmacological activity research, and can also be used for preparing anti-inflammatory drugs.

The invention relates to the technical field of fungus active ingredient extraction and medical application, and particularly provides a preparation method and application of phellinus igniarius phenol A. The preparation method comprises the following steps: extracting phellinus igniarius with an ethanol water solution and ethyl acetate in sequence to obtain an extract; the extract is subjected to silica gel column chromatography, Sephadex LH-20 (MeOH) column chromatography and ODS semi-preparative column separation in sequence, and the phellinus igniarius phenol A is obtained. In-vivo animal experiments prove that the phellinus igniarius phenol A has certain in-vitro inhibitory activity on six tumor cells, namely NCI-H460, MKN-45, 22Rv1, MCF7, SiHa and Hep 3B2.1-7, and particularly has the most remarkable in-vitro inhibitory effect on liver cancer Hep3B2.1-7 cells.

The present application relates to a kind of saponins HZ extracted from shi medicine twelve hours and its preparation method and application.Twelve hours saponins HZ is prepared using the following method: with shi medicine twelve hours as raw material, using impregnation extraction, extraction, microporous resin MCI column chromatography separation to obtain extract Fr.B4, extract Fr.B4 is purified by Sephadex LH-20, obtain FrB4-1~FrB4-6 6 components, component FrB4-4 is separated by semi-preparative high performance liquid chromatography (CH3CN:H2O=30:70, 3.0mL / min) to obtain twelve hours saponins HZ.Twelve hours saponins HZ has significant effect in the treatment of ulcerative colitis, and can be further used for developing safe, effective, controllable traditional Chinese medicine anti-inflammatory, ulcerative colitis new drug.

The invention relates to the technical field of traditional Chinese medicine extraction and separation, in particular to an extraction and separation method of a pyridine alkaloid compound in purslane and application of the pyridine alkaloid compound. The molecular formula of the pyridine alkaloid compound is C6H8N2O, and the pyridine alkaloid compound is named as 6-metaxyridin-3-amine. According to the method, ethyl alcohol reflux extraction, ODS column, Sephadex LH-20, a high performance liquid chromatograph and the like are adopted for separation, purification and preparation, 6-methoxyridin-3-amine is successfully extracted and separated, the method is easy to operate, the operation method is simple, convenient and rapid, ethyl alcohol and methyl alcohol are mainly adopted for extraction in the extraction and separation process, the technological method is environmentally friendly, and the method is suitable for industrial production. The purity of the compound separated by the method is higher than 99%, and research shows that the compound has an anti-inflammatory effect, so that the pyridine alkaloid compound and the salt and the derivative thereof can be used as synthesis primers of other compounds and raw materials for new drug development and pharmacological activity research, and can also be used for preparing anti-inflammatory drugs.

The present invention relates to diterpenoid alkaloid compounds in Delphinium aemulans Nevski var. aemulans and a preparation method and use thereof. The compounds use Delphinium aemulans Nevski var. aemulans as a raw material, are extracted with a solvent, treated with acid and base, subjected to solvent extraction, and separated by two, three or four of silica gel column chromatography, Sephadex LH-20 column chromatography, preparative thin layer chromatography or high performance liquid chromatography, and detected and analyzed by thin layer chromatography or high performance liquid chromatography to obtain two new compounds: Compound 1 named aemulanosine A; Compound 2 named aemulanosine B. The voltage-gated potassium channel activities of the two compounds were measured. The experimental results show that aemulanosine A and aemulanosine B isolated from Delphinium aemulans Nevski var. aemulans show inhibitory effects on voltage-gated potassium channel currents and can be used for the preparation of drugs with the activity of inhibiting voltage-gated potassium channel currents.

The invention provides a ferulate compound as well as a preparation method and application thereof, the ferulate compound is (5-formyl-2-furan) methyl dihydroferulate, the ferulate compound is prepared by the following steps: extracting a dry whole herb water extract of eyebright by using ethyl acetate, recovering a solvent to obtain an ethyl acetate extraction part, and separating by using normal-phase silica gel column chromatography to obtain the (5-formyl-2-furan) methyl dihydroferulate. Petroleum ether-ethyl acetate is used as an eluent for gradient elution, a fraction Fr.2 is obtained and separated by normal-phase silica gel column chromatography to obtain a fraction Fr.2. 2, Sephadex LH-20 gel column chromatography is performed to obtain a fraction Fr.2. 2.1, MC I column chromatography is performed to separate the fraction Fr.2. 2.1, methanol-water is used as an eluent for gradient elution to obtain a fraction Fr.2. 1.1, and then preparative HPLC (High Performance Liquid Chromatography) is performed to obtain the HaCaT cell activity. The composition has a protection effect on HaCaT cell oxidative damage caused by UVB, and can effectively resist skin photoaging.

The present invention belongs to the technical field of polysaccharide extraction. More specifically, it relates to the extraction and application of Liubao tea polysaccharide with in vitro antioxidant activity, blood glucose-lowering and blood lipid-lowering activities. The present invention obtained the Liubao tea polysaccharide component TPS-5 by extraction with water extraction and alcohol precipitation methods, purification with DEAE-52 cellulose column and Sephadex G-200 gel column, and identified and analyzed its structure. In vitro biological activities confirmed that TPS-5 has antioxidant, bile salt-binding, α-amylase and α-glucosidase inhibitory activities. HepG2 cell experiments proved that TPS-5 has good effects on lowering blood glucose, blood lipid and cholesterol. These results indicate that TPS-5 can be used as a natural antioxidant resource to regulate blood lipid and blood glucose, and may also be used as a natural ingredient for treating diabetes. The present invention lays a theoretical foundation for the application of functional active Liubao tea polysaccharide in health foods or functional foods.

The invention relates to a preparation method and application of a flavonoid diglycoside compound which is separated from a dry whole plant of daphne tenuifolia plant and has antineoplastic activity. The preparation method comprises the following steps: taking a dry whole plant of a daphne plant daphne gemma E.Pritz. Ex Diels as a raw material, and preparing a raw material from the dry whole plant, namely the daphne gemma E.Pritz. Ex Diels, of the daphne gemma E.Pritz. Ex Diels, of the daphne gemma E.Pritz. Ex Diels; the preparation method comprises the following steps: extracting an extract, extracting and concentrating, and then carrying out various separation methods such as silica gel column chromatography, reversed-phase ODS column chromatography and Sephadex LH-20 to obtain the flavonoid diglycoside compound: # imgabs0 #. The cytotoxic effects of the compounds I-III on three human cancer cell strains of lung cancer A549, breast cancer MDA-MB-231 and colon cancer SW480 are evaluated by adopting an MTS method; the compounds have strong anti-lung cancer and anti-breast cancer activity, and have potential anti-tumor drug research and development and application values.

The invention discloses a scallop skirt umami peptide as well as a preparation method and application thereof. The preparation method comprises the following steps: cleaning fresh scallops, opening shells, taking skirts, removing visceral clusters, adding water, and homogenizing to obtain scallop skirt homogenate; adding water into the scallop skirt homogenate, sequentially adding flavourzyme and compound protease, performing enzymolysis, performing enzyme deactivation after enzymolysis, performing centrifugation, collecting supernate, performing vacuum concentration, and performing freeze drying to obtain scallop skirt zymolyte; separating and purifying the scallop skirt zymolyte by adopting a Sephadex LH-20 sephadex column to obtain a sephadex column separation component containing the scallop skirt umami peptide; performing LC-MS / MS identification on the sephadex column separation component containing the scallop skirt umami peptide to obtain an amino acid sequence of the scallop skirt umami peptide; the scallop skirt umami peptide is verified by using a molecular docking technology and sensory evaluation. The prepared scallop skirt umami peptide has good umami, has great application significance in freshness enhancing food additives, is a small-molecular-weight peptide, and is easy to absorb by human bodies.