86 results about "Sephadex" patented technology

The invention discloses a novel benzofuran component in eupatorium chinense and application of the benzofuran component in anti-inflammatory activity. The obtained compound is separated from an eupatorium chinense extract. The eupatorium chinense root extract is separated by using an extraction method, macroporous adsorption resin impurity removal, Sephadex LH-20 gel column chromatography, high performance liquid chromatography and other methods. According to the present invention, all the separated compounds and the analogues thereof have good inhibition activity on target cyclooxygenase-2 of inflammation-related diseases, and have good inhibition activity on COX-2 enzyme, and the molecular docking experiment results show that the compounds and the target proteins have good binding energy. Therefore, the compound can be used for preparing drugs for treating inflammatory related diseases, or anti-inflammatory and analgesic drugs, or tumor drugs by inhibiting COX-2 enzyme, such as drugs for rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, Alzheimer's disease and the like, and related natural lead compounds are provided.

The invention relates to the technical field of biological medicines, in particular to a polycyclic polyisopentenyl cyclopentanone compound as well as a preparation method and application thereof. The polycyclic polyisopentenyl cyclopentanone compound provided by the invention has a structural formula as shown in a formula 1; the preparation method comprises the following steps: by taking dry fruits of hypericum berry as raw materials, carrying out percolation extraction and concentration to obtain an extract, suspending the extract in water to obtain a suspension, and extracting and concentrating the suspension with petroleum ether to obtain a crude extract; the crude extract is taken and subjected to positive and negative phase silica gel column separation, MCI medium-pressure column chromatography, Sephadex LH-20 gel column chromatographic separation, high performance liquid chromatography preparation and CHIRALPAK IG chiral column resolution, and the polycyclic polyisopentenyl cyclopentanone compound is obtained. The protection activity of the compound on AC16 myocardial cell injury induced by cold ischemia is evaluated, and the compound can be used for preparing the polycyclic polyisopentenyl cyclopentanone compound. The compounds can be used as lead compounds for developing medicines for preventing and treating myocardial cold ischemia injury.

The invention relates to the technical field of natural medicines, in particular to a 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound as well as a preparation method and application thereof. The 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound disclosed by the invention has a structure as shown in a formula I; the method comprises the following steps: extracting hypericum monogynum by using ethanol and petroleum ether, sequentially eluting the obtained petroleum ether extract through silica gel column chromatography, MCI resin column chromatography, Sephadex LH-20 gel column chromatography and ODS medium-pressure column chromatography, and purifying the collected eluate through preparative ODS liquid chromatography and semi-preparative ODS liquid chromatography to obtain the 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound. The 3 / 6 / 5 / 6 / 5 pentacyclic phloroglucinol compound is extracted from hypericum monogynum for the first time, and the compound has great development value as a novel medicine for preventing or treating calcified aortic valve diseases.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

The application provides a momordica root polysaccharide and a preparation method and application thereof, and belongs to the technical field of natural product development. The preparation method of the momordica root polysaccharide comprises the following steps: drying and crushing momordica roots, and removing fat by ethanol reflux; after water extraction and alcohol precipitation, and removing protein, a momordica root crude polysaccharide is obtained; and the momordica root crude polysaccharide is sequentially separated and purified by DEAE-52 cellulose column and Sephadex LH20 gel column to obtain the momordica root refined polysaccharide. The preparation method has the advantages that the polysaccharide obtained by directly extracting water in the prior art has a narrow molecular weight distribution range and better immune enhancement activity. Experimental results show that the momordica root polysaccharide can stimulate RAW264.7 cells to secrete NO, TNF-alpha and IL-6 factors, enhance the phagocytosis of RAW264.7 cells, and thus enhance the cell immune function, and can be applied to the development of products for improving immunity.

The invention provides a traditional Chinese medicine composition for reducing blood sugar and blood pressure and a preparation method thereof, and belongs to the technical field of biological medicine, the composition comprises ivory white eggplant extract, parsley extract, banana zucchini rattan extract, coloured bean puffball extract, eucommia ulmoides glycoside and puerarin. The preparation method comprises the following steps: carrying out common enzymolysis on ivory white eggplant by using cellulase, pectinase and papain, and purifying by using an XAD-16 macroporous resin column and a Sephadex LH-20 gel column to obtain an ivory white eggplant extract; the method comprises the following steps: extracting parsley with an ethanol aqueous solution, and purifying with an AB-8 macroporous adsorption resin column to obtain a parsley extract; the preparation method comprises the following steps: extracting banana zucchini rattan by using an ethanol water solution, and purifying by using a D101 macroporous adsorption resin column to obtain a banana zucchini rattan extract; colored bean puffball is subjected to common enzymolysis with chitinase and ficin, extracted with a two-phase solvent and purified with an HPD-100 macroporous adsorption resin column, and the colored bean puffball extract is prepared. All the components are matched to realize synergy of reducing blood sugar and blood pressure.

The invention discloses neutral refined polysaccharide of longan aril from Guangxi, and belongs to the technical field of bioengineering. The invention particularly relates to a preparation and characterization method and structural analysis of the high-purity longan pulp neutral refined polysaccharide LYP-1-s. The polysaccharide is separated and purified from longan pulp through a water extraction and alcohol precipitation method in combination with DEAE-FF and Sephadex G-75 column chromatography, the yield is 4.81 + / -0.06%, and the purity is high. TLC and ultraviolet full spectrum analysis results show that LYP-1-s is high in component enrichment degree, few in impurities, high in activity and uniform in molecular weight distribution. The polysaccharide is neutral polysaccharide, is easily soluble in water and is insoluble in organic solvents such as ethanol. In the digestion process, stable antioxidant activity can be kept, and certain life benefiting activity is shown on intestinal flora. Therefore, LYP-1-s has application potential as a novel health food raw material, and can be used for development of related industries.

The invention relates to the technical field of traditional Chinese medicine extraction and separation, in particular to two alkaloid compounds in portulaca oleracea and an extraction and separation method and application thereof. The molecular formulas of the two kinds of alkaloid compounds are both C27H33NO15, and the two kinds of alkaloid compounds are respectively named as oleraclamie K and oleraclamie L. The invention further discloses a preparation method of the oleraclamie. According to the method, water extraction, ODS column chromatography, Sephadex LH-20, a high performance liquid chromatograph and the like are adopted for separation, purification and preparation, the compounds oleraclamie K and oleraclamie L are successfully extracted and separated out, the method is simple, convenient and rapid, the extraction and separation process mainly adopts a water extraction process, the method is environmentally friendly, the purity of the compounds separated through the method is high and larger than 90%, and the method is suitable for industrial production. In addition, researches show that the two compounds have anti-inflammatory effects, so that the alkaloid compound and the salt and the derivative thereof can be used as synthesis primers of other compounds and raw materials for new drug development and pharmacological activity research, and can also be used for preparing anti-inflammatory drugs.

The present application relates to a kind of saponins HZ extracted from shi medicine twelve hours and its preparation method and application.Twelve hours saponins HZ is prepared using the following method: with shi medicine twelve hours as raw material, using impregnation extraction, extraction, microporous resin MCI column chromatography separation to obtain extract Fr.B4, extract Fr.B4 is purified by Sephadex LH-20, obtain FrB4-1~FrB4-6 6 components, component FrB4-4 is separated by semi-preparative high performance liquid chromatography (CH3CN:H2O=30:70, 3.0mL / min) to obtain twelve hours saponins HZ.Twelve hours saponins HZ has significant effect in the treatment of ulcerative colitis, and can be further used for developing safe, effective, controllable traditional Chinese medicine anti-inflammatory, ulcerative colitis new drug.

The invention relates to the technical field of traditional Chinese medicine extraction and separation, in particular to an extraction and separation method of a pyridine alkaloid compound in purslane and application of the pyridine alkaloid compound. The molecular formula of the pyridine alkaloid compound is C6H8N2O, and the pyridine alkaloid compound is named as 6-metaxyridin-3-amine. According to the method, ethyl alcohol reflux extraction, ODS column, Sephadex LH-20, a high performance liquid chromatograph and the like are adopted for separation, purification and preparation, 6-methoxyridin-3-amine is successfully extracted and separated, the method is easy to operate, the operation method is simple, convenient and rapid, ethyl alcohol and methyl alcohol are mainly adopted for extraction in the extraction and separation process, the technological method is environmentally friendly, and the method is suitable for industrial production. The purity of the compound separated by the method is higher than 99%, and research shows that the compound has an anti-inflammatory effect, so that the pyridine alkaloid compound and the salt and the derivative thereof can be used as synthesis primers of other compounds and raw materials for new drug development and pharmacological activity research, and can also be used for preparing anti-inflammatory drugs.

The invention discloses a scallop skirt umami peptide as well as a preparation method and application thereof. The preparation method comprises the following steps: cleaning fresh scallops, opening shells, taking skirts, removing visceral clusters, adding water, and homogenizing to obtain scallop skirt homogenate; adding water into the scallop skirt homogenate, sequentially adding flavourzyme and compound protease, performing enzymolysis, performing enzyme deactivation after enzymolysis, performing centrifugation, collecting supernate, performing vacuum concentration, and performing freeze drying to obtain scallop skirt zymolyte; separating and purifying the scallop skirt zymolyte by adopting a Sephadex LH-20 sephadex column to obtain a sephadex column separation component containing the scallop skirt umami peptide; performing LC-MS / MS identification on the sephadex column separation component containing the scallop skirt umami peptide to obtain an amino acid sequence of the scallop skirt umami peptide; the scallop skirt umami peptide is verified by using a molecular docking technology and sensory evaluation. The prepared scallop skirt umami peptide has good umami, has great application significance in freshness enhancing food additives, is a small-molecular-weight peptide, and is easy to absorb by human bodies.

The invention relates to the technical field of biological medicine, and discloses a yak milk casein antioxidant peptide as well as a preparation method and application thereof, and the preparation method comprises the following steps: step 1, dissolving yak milk casein in alkali liquor to obtain a casein solution; step 2, carrying out enzymolysis on the casein solution by using protease to obtain enzymatic hydrolysate; step 3, inactivating and centrifuging the enzymatic hydrolysate, taking supernate, and freeze-drying to obtain a crude polypeptide product; and step 4, purifying the polypeptide crude product through anion exchange column chromatography and gel filtration chromatography in sequence to obtain the antioxidant peptide component. The preparation method is efficient and clear in target, yak milk casein is subjected to enzymolysis through a single enzyme / complex enzyme combined strategy, a two-step purification process of DEAE-52 cellulose column chromatography and SephadexG-50 gel chromatography is combined, the high-activity polypeptide component is efficiently and repeatedly prepared, and the defect that traditional enzymolysis product components are complex is overcome.

This invention relates to the fields of biomedicine and microbial fermentation engineering technology, and discloses a strain of *Streptomyces citrus* YS-4 that produces mesocyclocarpine. The separation and purification process includes: solid-liquid separation of the fermentation broth; extraction of the supernatant with ethyl acetate and extraction of the bacterial cells by methanol soaking; combining and concentrating the extracts to obtain a crude extract; purification of the crude extract by silica gel column chromatography and Sephadex LH-20 gel column chromatography; and finally purification by C18 reversed-phase semi-preparative high-performance liquid chromatography to obtain mesocyclocarpine. This invention employs a dual extraction strategy to improve the yield and achieves efficient separation of the target product and its structural homologues through specific chromatographic conditions, effectively solving the problem of incomplete extraction caused by the coexistence of intracellular adsorption and extracellular secretion of the target product, resulting in a final product yield superior to that of single-solvent extraction methods.

The invention provides a high-purity bovine bone collagen peptide gel filtration chromatography purification method, and belongs to the technical field of bioactive peptide purification. The method comprises the following five core steps: pretreatment, gel column preparation, dynamic equilibrium, sample loading and elution, and post-treatment: firstly, carrying out ultrafiltration pretreatment on a bovine bone collagen peptide crude extract, then filling a chromatographic column with a specific diameter-height ratio by using cross-linked sephadex, carrying out dynamic equilibrium by using an accurately adapted equilibrium liquid after column efficiency verification, and finally, carrying out dynamic equilibrium by using a chromatographic column with a specific diameter-height ratio. The sample loading amount is dynamically matched through a formula, a gradient isothermal mode is adopted in the elution process, and finally a high-purity product is obtained through freeze drying. Through multi-step parameter collaborative optimization and precise regulation and control of a self-created formula, the problems of low purification efficiency, poor product purity and wide molecular weight distribution in the prior art are solved, the purity of the obtained bovine bone collagen peptide is greater than or equal to 98%, the proportion of peptide fragments with molecular weight distribution of 500-3000Da and 1000-2000Da is greater than or equal to 70%, the heavy metal content and the colony count both meet high-standard requirements, the yield is stabilized at 52% or above, and the method is suitable for industrial production. Good industrial application prospects are realized.

The invention discloses a benzylisoquinoline alkaloid glycoside compound as well as a preparation method and application thereof. The preparation method comprises the following steps: soaking tubers of rhizoma corydalis in a 6% acetic acid solution, crushing, soaking in water, and carrying out ultrasonic extraction to obtain a plant extract; after water dispersion, carrying out HPD100 type macroporous resin column chromatography separation, carrying out gradient elution by using ethanol aqueous solutions with different concentrations, and collecting a 50% ethanol elution part to obtain an elution part YH-D; carrying out MCI column chromatography separation, and carrying out gradient elution by using a 0-100% methanol-water solution to obtain four parts of eluents; the preparation method comprises the following steps: dissolving YH-D-2 in water, filtering, and carrying out medium-pressure ODS column chromatography separation, Sephadex LH-20 column chromatography separation, medium-pressure ODS column chromatography separation and semi-preparative C18 column HPLC (High Performance Liquid Chromatography) purification on the filtrate to obtain six new compounds 1-6. In-vitro experiments prove that the elution part YH-D-2 containing benzylisoquinoline alkaloid glycoside of the benzylisoquinoline alkaloid glycoside compounds 1-6 has NO generation inhibition activity, and has a good development prospect as an anti-inflammatory drug.

The invention provides a pharmaceutical composition for reducing uric acid and a preparation method thereof, and belongs to the technical field of biological pharmacy, and the active ingredients of the pharmaceutical composition comprise an okra seed extract, a carrot leaf extract, a red sword bean extract, quercetin and resveratrol. The abelmoschus esculentus seed extract is prepared by performing compound enzymolysis on abelmoschus esculentus seeds by cellulase, pectinase and xylanase, fermenting the abelmoschus esculentus seeds by rhizopus oryzae to obtain an extracting solution, and purifying the extracting solution by a D101 macroporous adsorption resin column and a polyamide chromatographic column. The carrot leaf extract is a component which is obtained by extracting carrot leaves with an ethanol water solution and is less than 10kDa. The red sword bean extract is prepared by the following steps: degreasing red sword bean seeds, carrying out compound enzymolysis on the degreased red sword bean seeds by using neutral protease and beta-glucosidase, fermenting the degreased red sword bean seeds by using lactobacillus plantarum to obtain an extracting solution, and processing the extracting solution by using a Sephadex G25 gel column. All the components are compatible to construct a multi-component, multi-target and multi-channel action system, and good acid reduction is realized by inhibiting uric acid to generate key enzyme and regulating purine metabolism.

The application provides an Euphausia superba immunomodulatory peptide, which is prepared by alkaline protease and flavour protease for enzymolysis, and then the enzymolysis product is purified by cation exchange chromatography, Sephadex G25 gel chromatography and C18 reverse phase high performance liquid chromatography. The amino acid sequence of a polypeptide is SEQ ID NO: 1 or SEQ ID NO: 2. The Euphausia superba is used as raw material, Euphausia superba peptides are obtained by enzymolysis, and the amino acid sequences of the Euphausia superba peptides are analyzed and identified by LC-MS / MS after step-by-step purification. With the help of an activity prediction tool and molecular docking, a target peptide with good immunomodulatory activity potential is screened. The target peptide is artificially synthesized, the immunomodulatory activity is verified, and the action mechanism is further clarified. Data support is provided for the prediction screening and structure-activity relationship research of Euphausia superba source high-efficiency immunomodulatory peptides.

The invention provides an anti-atherosclerosis lipidosome carrier drug and a preparation method thereof, and belongs to the technical field of biological pharmacy, the lipidosome carrier drug is prepared by taking tussah peptide, premna microphylla leaf flavonoid glycoside, clausena lansium leaf polysaccharide and troxerutin as active ingredients. The antheraea pernyi peptide is a product between 1kDa and 5kDa obtained by extracting protein from antheraea pernyi and sequentially carrying out enzymolysis by bacillus subtilis protease and pepsin. The premna microphylla leaf flavonoid glycoside is prepared by performing enzymolysis and water extraction on premna microphylla leaves and then purifying the premna microphylla leaves through an HPD-600 macroporous adsorption resin column. The clausena lansium leaf polysaccharide is prepared by the following steps: extracting clausena lansium leaves with water, precipitating with ethanol, redissolving, and purifying through a DEAE-52 cellulose column and a Sephadex G-100 gel column in sequence. All the components aim at key pathological links of atherosclerosis respectively, and the core advantages of high efficiency, safety and stability are achieved through multi-component synergistic compatibility and closed-loop optimization of a fine preparation process.

The invention belongs to the technical field of comprehensive utilization of oyster mushrooms, and provides an oyster mushroom active peptide for inhibiting foaming of macrophages and a preparation method of the oyster mushroom active peptide. The method comprises the following steps: taking oyster mushroom as a raw material, preparing oyster mushroom protein through alkali extraction and acid precipitation, preparing a crude product of the oyster mushroom active peptide for inhibiting foaming of macrophages through compound enzymatic hydrolysis of the oyster mushroom protein by pepsin, trypsin and chymotrypsin, and separating and purifying the crude product by adopting ultrafiltration membrane ultrafiltration and Sephadex G-15 gel column chromatography to obtain the peptide with good activity and high purity. The bioactive peptide can reduce lipid accumulation, ROS level, TNF-alpha content and IL-6 content in RAW264.7 foamed macrophages, and effectively improve lipid accumulation, oxidative stress and inflammatory state of the foamed macrophages, thereby inhibiting foaming of the macrophages.

This invention discloses a method for extracting and preparing polysaccharides from the traditional Chinese medicine *Cat Ginseng* and its applications. The process involves drying and pulverizing the raw material, defatting it with 95% ethanol, and then using ultrasound-assisted extraction. The liquid-to-solid ratio, extraction time, and ultrasonic power are optimized using response surface methodology. The extract is then centrifuged, concentrated, precipitated with alcohol, deproteinized and decolorized using macroporous resin, dialyzed, and freeze-dried to obtain crude polysaccharides. These crude polysaccharides are then purified by DEAE-52 and Sephadex G-150 column chromatography to obtain three homogeneous new polysaccharide fractions: AvPs1-1, AvPs2-1, and AvPs2-2. The polysaccharide extract, dominated by AvPs2-1 and AvPs2-2, not only exhibits protective effects on zebrafish embryonic development but also demonstrates significant anti-fibrotic activity in a liver injury model. Due to its natural non-toxicity, water solubility, and multiple functions including embryonic development protection and anti-fibrosis, this polysaccharide shows broad application prospects in functional foods, health products, and therapeutic drugs.

The invention discloses a pentacyclic triterpenoid compound with anti-tumor activity and a preparation method and application thereof, and belongs to the technical field of medicines, and the structure of the compound is confirmed through comprehensive spectrum analysis. The preparation method comprises the following steps: carrying out reflux extraction on dried euphorbia pekinensis roots with ethanol, concentrating, extracting with dichloromethane, and carrying out multi-step separation and purification steps such as system combination silica gel column chromatography, medium-pressure silica gel column chromatography, Sephadex LH-20 gel chromatography and preparative high performance liquid chromatography to finally obtain the high-purity compound. In-vitro anti-tumor activity experiments show that the compound has a remarkable proliferation inhibition effect on mouse liver cancer cells Hepa1-6. The invention provides a lead compound with a novel structure for developing a novel anti-hepatoma drug.

The invention discloses a novel flavonoid compound as well as a preparation method and application thereof, and belongs to the technical field of natural medicines. The preparation method comprises the following steps: heating, refluxing and extracting with ethanol, and concentrating into thick extract under reduced pressure to obtain a crude extract; step 2, extraction; step 3, loading the dichloromethane extract on an MCI column; 4, concentrating the 30% methanol eluent to prepare a liquid phase; and step five, purifying the compound crude product (I) and the compound crude product (II) through a Sephadex LH-20 gel column respectively, and eluting with methanol to obtain the compound (I) and the compound (II) respectively. According to the invention, two new flavonoid compounds are separated from the pogonatum insignis plant, and the two new compounds have good anti-gastric cancer activity, so that a foundation is laid for development and utilization of pogonatum insignis plant resources.

The invention relates to the technical field of natural product separation engineering, discloses a method for synchronously separating and purifying multiple active components of camellia oleifera shells based on synergistic extraction-gradient desorption-targeted refining, and aims to solve the technical bottleneck that high-purity tannin, proanthocyanidin and tea saponin cannot be efficiently and synchronously obtained from the camellia oleifera shells in the prior art. The core innovation is as follows: 1) an ultrasonic-microwave synergistic extraction parameter matching system is designed, and efficient synergistic dissolution of three active components is realized through precise matching of ultrasonic power and microwave power (0.5-1.7): 1 in combination with a 65-75% ethanol aqueous solution; (2) a three-stage incremental ethanol concentration gradient elution strategy is innovatively adopted, and directed preliminary separation of component groups is realized through step-by-step elution of 20-30% ethanol, 50-60% ethanol and 80-95% ethanol according to polarity differences of the three components; and (3) establishing a targeted refining technology combination, adopting Sephadex LH-20 gel chromatography according to the molecular weight characteristics of proanthocyanidin, and adopting high-speed counter-current chromatography of an ethyl acetate-n-butyl alcohol-water system in a specific ratio according to the structural characteristics of tea saponin homologues to realize deep purification. The continuous synchronous preparation of three active components in the camellia oleifera shells is realized for the first time, the purity of tannin in the obtained product is greater than or equal to 75%, the purity of proanthocyanidin is greater than or equal to 90%, and the purity of tea saponin is greater than or equal to 92% (the proportion of oleanane saponin is greater than or equal to 85%), the utilization rate of raw materials is increased by 30% or above, and the solvent consumption is reduced by 25%. An efficient, economical and environment-friendly integrated technical scheme is provided for high-value utilization of the camellia oleifera shells, and the method has a remarkable industrial application value.

The invention discloses a perinereis aibuhitensis sourced immunoactive peptide PP and application thereof, and belongs to the technical field of bioactive peptides. According to the invention, lt; the method comprises the following steps: by taking a 5 kDa nereis enzymatic hydrolysate as a raw material, separating and purifying through Sephadex G-25 gel chromatography to obtain a nereis purified component, infecting nematodes by utilizing pseudomonas aeruginosa PA14 to establish an immunocompromised nematode model, and evaluating the immunocompetence of the nereis purified component by measuring the life, exercise ability, reproductive ability and lipofuscin accumulation indexes of the nematodes. A peptide spectrum of a polypeptide component with optimal immunocompetence is represented by combining a polypeptiomics technology, immunocompetence peptide is rapidly screened out by combining bioinformatics and a molecular docking technology, finally, nereis active peptide is obtained through chemical synthesis, the immunocompetence is verified by utilizing nematodes infected by PA14, and a reference is provided for rapid screening of the food-borne immunocompetence peptide.