880 results about "Sephadex" patented technology

The invention discloses a sea cucumber polypeptide, wherein a preparation method thereof comprises the following steps: (1) getting fresh stichopus japonicus, removing entrails, cleaning and crushing the stichopus japonicus into small blocks, and adding water to form homogenate; after the enzymolysis of the homogenate liquid, centrifugating and getting the supernatant; adding ethanol, standing, centrifugating and getting the supernatant, concentrating the supernatant at reducing pressure and drying the concentrate to form a crude product of the sea cucumber polypeptide; (2) dissolving the crude product of the sea cucumber polypeptide by using distilled water, carrying out gel filtration chromatography by Sephadex LH-20, eluting the sea cucumber polypeptide by double-distilled water, getting a second peak, collecting active components, and condensing, freezing and freeze-drying the active components; and (3) dissolving the active components by double-distilled water, carrying out ion exchange chromatography by Q Sepharose Fast Flow, linearly eluting the active components by NaCl solution, desalting, getting the second peak, collecting the active components, and condensing, freeze-drying the active components to form the sea cucumber polypeptide. The sea cucumber polypeptide can be used for preparing medicaments or health-care products for increasing leukocytes and also can be used for preparing medicaments or health-care products for multiplying marrow cells after chemotherapy.

The invention discloses a method of extracting sulforaphen. The smashed seeds of west orchids are added with two to three times as much water, the pH value is regulated to 3.8 to 4.2, ascorbic acid is added, and hydrolysis lasts seven to nine hours under the temperature of 20 to 30 DEG C; after being refrigerated and dried, the raw solution is added with fifteen to twenty five times as much propanone, supersonic extraction lasts forty to eighty minutes, and pumping filtration or double-gauze filtration follows; under the temperature of 30 to 50 DEG C, filtrate undergoes vacuum concentration in order to obtain crude sulforaphen; the crude sulforaphen is chromatographed by a silica gel column and gradiently eluted by n-hexane acetone solution, the thin-layer chromatography or HPLC tests and determines fractions containing the sulforaphen, and the crude sulforaphen undergoes vacuum concentration under the temperature of 30 to 50 DEG C; after being resolved in a small amount of acetone, the crude sulforaphen is chromatographed by a Sephadex LH-20 column, the acetone is used as eluent, the thin-layer chromatography or HPLC tests, determines and collects liquor containing the sulforaphen, and by vacuum concentration under the temperature of 30 to 50 DEG C, a sulforaphen product with eighty percent purity is produced. The method of the invention can also be used to extract the sulforaphen from the seeds of radishes, cabbages and mustards.

The invention discloses a giant salamander active peptide and an application. The giant salamander active peptide is prepared by the following steps: conducting enzymatic hydrolysis on giant salamander meat which serves as a raw material by virtue of a complex enzyme of marine alkaline protease and papain; separating an enzymatic hydrolysate by virtue of a trypsin immobilized ultra-filtration membrane separator; and then conducting separation and purification by virtue of Sephadex LH-20 molecular sieve chromatography and high performance liquid chromatography, so that the giant salamander active peptide is obtained. The giant salamander active peptide is capable of effectively scavenging free radicals and boosting body immunity, and meanwhile, the active peptide can also promote proliferation of skin fibroblasts; therefore, the giant salamander active peptide has a broad application prospect in the fields of food, medicines and cosmetics.

The present invention is sand sage polysaccharide, prepared with sand sage seed deoiling dreg as material and through extraction, separation, enzymolysis and chromatographic separation, and its preparation process and application. The preparation process features 50-80 concentration alcohol solution extraction, extraction with mixed chloroform and normal butyl alcohol liquid, enzymolysis with papain, and chromatographic separation in sephadex G-20 column. Sand sage polysaccharide is used in treating alloxan diabetes, reducing the damage of liver and kidney caused by diabetes, lowering the content of glutamic-oxaloacetic transminase, glutamic-pyruvic transminase and urea nitrogen in blood, and inhibiting diabetes caused weight loss.

The invention discloses an oyster polysaccharide (alpha-1, 4; 1, 6-gluglucosan and beta-1, 6-gluglucosan with relative molecular weight at 4000-6500Da) and making method and application in the cosmetics, which comprises the following steps: washing Dalian bay oyster or Atlantic oyster through water at 0-10 deg.c; boiling oyster at 70-80 deg.c under 0.3-0.5 Mpa for 20-45min; filtering; collecting supernatant; centrifuging to remove sediment; adjusting pH value of supernatant to 5-6 through acetic acid; acidolyzing for 1-3h; intercepting material with molecular weight less than 30000Da through film separator; adjusting pH value of filtrate over 30000Da to 6-8 through NaOH; centrifuging to remove sediment; dialyzing supernatant in the distilled water; passing tomographic column of Sephadex G-100 molecular sieve; collecting material at adsorbing peak with OD at 280 nm; freezing; drying.

The invention provides a triple-enzyme hydrolysis preparation method of anti-tumor polypeptides of spirulina. The method comprises the following steps: preparing a solution having a concentration of 5 percent from spirulina powder with ultrapure water; repeatedly freezing and thawing, homogenizing and performing ultrasonic treatment, centrifuging to obtain supernate, freezing and drying for later use; adding the prepared protein fluid into pepsin for enzyme hydrolysis, controlling the pH value, adding trypsin for enzyme hydrolysis, adding chymotrypsin for enzyme hydrolysis, controlling the pH value, deactivating enzyme, cooling, and centrifuging to obtain supernate; sequentially filtering with ultrafiltration membranes having molecular weight cut-off of 10KD, 5KD and 3KD respectively to obtain three kinds of spirulina protein enzymatic hydrolysates; carrying out sephadex G15 column chromatography on 0-3K of enzymatic hydrolysate, eluting with water, and collecting four polypeptide ingredients, namely anti-tumor polypeptides of spirulina. The obtained spirulina polypeptide and monopeptide ingredients can be used for establishing a theoretical basis for development and utilization of anti-tumor medicines and health foods.

The invention discloses a mytilus edulis enzymolysis polypeptide. The mytilus edulis enzymolysis polypeptide is characterized by containing the following amino acid sequence: Asp Leu Tyr. The mytilus edulis enzymolysis polypeptide is prepared by adopting the following steps of: (1) preparing homogenate from mytilus edulis meat, adding alkaline protease, deactivating the protease, centrifuging, and taking clear solution of the upper layer; (2) performing ultra-filtration on the clear solution, collecting hydrolysate with the molecular weight of below 3K, concentrating, and performing freeze drying; (3) performing chromatographic separation by adopting a DEAE-SepharoseFF ion exchange column; (4) performing chromatographic separation by adopting a Sephadex G-25 gel column; and (5) performing high performance liquid chromatography purification. The invention also discloses application of the mytilus edulis enzymolysis polypeptide prepared by the steps in prostatic cancer resistance. Compared with the prior art, the invention has the advantages that: the mytilus edulis is subjected to enzymolysis and purification by adopting an optimal protease and an optimal technology, a strong cell proliferation inhibiting effect is achieved when the obtained target peptide is applied to prostatic cancer resistant cells, and a feasible research path is provided for resisting prostatic cancer.

The invention discloses a making method of high-purity tanshinone, which comprises the following steps: (1) heating and refluxing to extract salvia miltiorrhizae root and stalk through 0.1-6.0mol/L acid water/alcohol solution; (2) adsorbing extract through adsorbing column with large-hole resin adsorbent; (3) eluting through water until pH value is 7 first and hydrophilic solvent then; collecting 3-5 column bulks to elute tanshinone completely; (4) evaporating; adding organic solvent; heating; dissolving; crystallizing; or adopting polyamide, gluglucosan gel Sephadex LH-20, MCI GEL CHP20P, C4, C8 or C18 bond phase; obtaining the product.

The invention discloses a method for extracting polygahatous polysaccharides from traditional Chinese medicine rhizoma polygonati. The method comprises the steps of cleaning, drying and grinding the rhizoma polygonati; performing secondary microwave auxiliary extraction on rhizoma polygonati powder through hexanol serving as a solvent, filtering and combining secondary extraction liquid; recycling the hexanol from the combined extraction liquid, then concentrating the extraction liquid, adding ethyl alcohol, performing uniform stirring, putting into an ultrasonic oscillator for ultrasonic oscillation, standing, performing centrifugal filtration to obtain precipitates, and drying the precipitates to obtain coarse polygahatous polysaccharides; removing proteins from the coarse polygahatous polysaccharides through an ethanol solution, recycling ethanol, concentrating to concentrated paste, dissolving the concentrated paste into a Tris-HCl buffering solution, and purifying the polygahatous polysaccharides twice through DE-52 cellulose column chromatography; performing third-time purification through distilled water by SephadexG-100 sephadex gel filtration chromatography, concentrating and carrying out freeze drying to obtain a refined polygahatous polysaccharides product. The extraction method disclosed by the invention is simple and feasible; the production efficiency is high; the quality of the prepared product is high; the yield is over 90 percent, and the purity is over 99 percent; the method is suitable for industrial large-scale production.

The invention discloses a preparation method of a pure product of Salvianic acid A sodium, which comprises processing steps: 1. salvia miltiorrhizae is extracted by a hydrothermal method, and an extracting solution is condensed, deposited in alcohol and filtered; 2. a filtrate is condensed, deposited in alcohol and filtered; 3. the filtrate is condensed, added with water and filtered, and the filtrate is put into a macroporous absorption resin column and washed by water; 4. after a water lotion is condensed, the step is carried out in accordance with step (5); or the water lotion is put into a polyamide column and washed by water, and after the water lotion is condensed, the step is carried out according to step (5); 5. the water lotion after condensation is mixed with silicon gel, dried, column-packed and eluted by an organic solvent, and then an eluent is collected, recovered to be dry to obtain dry paste; and 6. the dry paste is taken and dissolved by the organic solvent, and PH value is adjusted, and then the material is stood and crystallized; or the dry paste is taken and dissolved by a solvent, and then put into a Sephadex LH-20 column or reversed-phase silica gel column. The PH value is adjusted by the eluent, and standing and crystallizing are carried out. The Salvianic acid A sodium prepared by the method has purity of more than or equal to 98 percent (standardized by NICPBP in respect of standard substances). Furthermore, the method has low cost, less pollution to environment and easily industrialized production.

The invention discloses a multi-flavone component extracting and purifying method from liquorice, which comprises the following steps: 1. extracting and condensing glycyrol liquid; 2. adsorbing through large-hole resin; eluting the water and alcohol gradient; 3. remaining eluent of different density alcohol to continue different technologies( freezing, adding concentrated sulfuric acid-carbinol to do reflux hydrolysis; adding 80-100 order polyamide column; eluting water and carbinol; passing Sephadex LH-20 column; eluting again to produce multi-flavone component within iquiritin, liquiritigenin, isoliquiritin and isoliquiritin glucose apiin).

The invention discloses lactobacillus casei bacteriocin and use thereof in feed. The lactobacillus casei provided by the invention is separated from Tibet plateau traditional fermented yak yogurt, and the produced bacteriocin is obtained by ammonium sulfate precipitation, Sephadex G-100 gel filtration and reversed-phase high-performance liquid chromatography (RP-HPLC). The bacteriocin has thermal and acid stability, can be degraded by protease and has a wide antibacterial spectrum. When the bacteriocin is added into feed, and the growth of bacteria can be inhibited. The bacteriocin has a bright prospect in use as a feed additive.

The invention discloses a preparation method of rice bran protein peptide with ACE inhibitory activity. The method is characterized in that rice bran is adopted as a raw material, and comprises the following steps: firstly extracting rice bran protein, then preparing rice bran protein hydrolysate by adopting an enzyme hydrolysis method, and separating and purifying the rice bran ACE inhibitory peptide by adopting a hyperfiltration method, sephadex G-15 and a RP-HPLC method. According to the preparation method, the rich and cheap rice bran resource is effectively utilized to prepare the rice bran protein peptide with the high ACE inhibitory activity. The decompression function of the rice bran ACE inhibitory peptide can be maximally utilized, so that on one hand, the additional value of the rice bran is increased, and the good economic benefit and market value can be created; on the other hand, the morbidity of the hypertension can be effectively reduced, the high morbidity can be controlled, the human beings can be protected from the harm of the hypertension, the application value and social benefit are good, and the theoretical foundation and the experiment evidence can be provided for researching the structure and decompression mechanism of the rice bran protein ACE inhibitory peptide.

The invention provides a preparation method of a small water turtle anti-tumor polypeptide. The method comprises the following steps: mixing 3 to 20g of meat paste of small water turtle and ultrapure water; agitating to obtain a mixed solution at concentration of 10 to 50% (w/v); adding trypsin to perform enzymolysis; deactivating enzyme after enzymolysis; cooling until reaching room temperature; centrifuging enzymatic hydrolysate; collecting supernate; sequentially filtering through ultrafiltration membranes which respectively have molecular cut off of 10KD, 5KD and 3KD, so as to obtain three enzymatic hydrolysates; performing sephadex G-15 column chromatography for 0-3K enzymatic hydrolysate; eluting with water; collecting four polypeptide components under a certain detection wavelength so as to obtain small water turtle anti-tumor polypeptide. The prepared small water turtle anti-tumor polypeptide is beneficial for the development and utilization of anti-tumor drugs and health foods.

The invention belongs to the field of marine organism small-molecular active peptides, and particularly relates to an anti-oxidation and DPP-IV inhibition active peptide derived from apostichopus japonicus by enzymolysis. The amino acid sequence of the anti-oxidation and DPP-IV inhibition active peptide is Ser-Arg-Pro-Gln-Tyr-Pro-Gln-Tyr-Pro-Ser. The anti-oxidation and DPP-IV inhibition active peptide is prepared by the following steps: adding water into fresh apostichopus japonicus for homogenizing, and placing the homogenate in an enzymolysis tank; performing enzymolysis with compound protease to obtain enzymatic hydrolysate; treating the enzymatic hydrolysate with a membrane separation technology to obtain a small-molecular active peptide crude product; separating the crude product through Sephadex LH-20 to obtain a small-molecular active peptide. The purity of the small-molecular active peptide as measured by adopting RP-HPLC (Reversed-Phase High Performance Liquid Chromatography)is greater than 95 percent, and the amino acid composition of the active peptide is measured by high performance liquid chromatography/mass spectrometry, so that the amino acid sequence of the small-molecular peptide is determined finally. The active small-molecular peptide derived from the apostichopus japonicus has the advantages of small molecular weight, simple separating-purifying steps, easiness in preparation, high purity and higher oxidization resistance and DPP-IV inhibition activity, has the characteristics of naturalness, safety and high efficiency, can be applied to the preventionand treatment of relevant diseases such as diabetes mellitus as an antioxidant as well as a DPP-IV inhibitor, and has a wide application prospect in the fields of foods, health care products and medicines.

The invention relates to a method for extracting and separating more than 90% iridoid glycoside compound monomer from efflorescence snake tongue grass, wherein the iridoid glycoside compound monomer of efflorescence snake tongue grass is E-6-0-coumaricacetate paederoside methyl ester. The method comprises the following steps: (1)putting the disintegrated efflorescence snake tongue grass in the primary alcohol aqueous solution in order to heat, reflux and extract; filtering; getting the concrete (I)by concentrating; adding the water in order to dissolve; filtering; getting the concrete (II)by concentrating; (2)dissolving the concrete (II)with the secondary alcohol aqueous solution; separating with macroreticular resin column; eluting with the tertiary alcohol aqueous solution; concentrating the eluent; getting the primary product; (3)proceeding with chromatography with Sephadex LH-20 column after dissolving the primary product with the fourth alcohol aqueous solution; proceeding with gradient elution with the fifth alcohol solution; concentrating; crystallizing; getting the product E-6-0-coumaricacetate paederoside methyl ester. The method can recycle the column material and solvent, which doesn't pollute the environment, has the low cost, the high product efficient and the easy operation, and is fit for the small-scale extraction separation of the laboratory and the industry extraction separation.

This invention relates to separation and purification method of catechin monomer EGCG and ECG. The features are that dextrane gel Sephadex LH-20 is column filling and eluant is absolute ethyl alcohol. Then column chromatography is made by dextrane gel Sephadex LH-20 and 40% ethanol aqueous solution be eluant, that is chromatography column non- gradient expendable separation is adopted. Comparing to original method, equipments are facilitated greatly and the method is simple, cost is low. The solvent is nontoxic and separation period is short, and monomer extraction rate and product purity are both high.

The invention discloses a method for extracting solanesol, cembrane diterpene, vitamin E and phytosterol from tobaccos simultaneously. The method comprises the steps that 1, extraction is carried out; 2, saponification is carried out, wherein saponified solanesol and other fat-soluble active ingredient extracts are obtained; 3, sephadex gel column separation is carried out, wherein solanesol, vitamin E and phytosterol (mixture) and cembrane diterpene crude extract are obtained respectively; 4, silica-gel column chromatography is carried out, wherein after silica-gel column chromatography, high-purity solanesol, vitamin E and phytosterol are obtained respectively; the obtained cembrane diterpene is subjected to silica-gel column chromatography, and alpha-4,8,13-cembratriene-1,3-diol and beta-4,8,13-cembratriene-1,3-diol are obtained respectively. Comprehensive extraction of tobacco fat-soluble active ingredients is achieved, tobacco residue obtained after fat-soluble active ingredients are extracted can be used for extraction of tobacco protein, chlorogenic acid, rutin, polysaccharide and nicotine, gradient utilization of tobacco active ingredients is achieved, and the utilization value of tobacco resources, particularly waste tobaccos is improved.