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Method for extracting sulforaphen

A technology of sulforaphane and ultrasonic extraction, applied in the field of biochemistry, can solve the problems of complex conversion mechanism of isothiocyanate, lack of extraction and purification technology, lack of in-depth research on how to control conversion conditions, etc.

Inactive Publication Date: 2008-03-19
ZHEJIANG GONGSHANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conversion mechanism of glucosinolates to isothiocyanates is complex, and how to regulate the conversion conditions is lack of in-depth research
Also lacks extraction and purification techniques

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] The present invention has carried out 10 kilograms of broccoli seed raw material extraction pilot tests, and its specific implementation technological process is as follows:

[0031] After crushing 1kg of broccoli seeds, add 2kg of water, mix and then add 200mL of 1mol / mL hydrochloric acid solution and 20mL of 1mg / mL ascorbic acid solution (pH4 at this time), seal it with plastic wrap after stirring, and place it at room temperature of 25°C Hydrolyzed for 8 hours, freeze-dried the hydrolyzed sample, added 20L acetone to the sample, and ultrasonically leached for 60 minutes in an ultrasonic instrument (ultrasonic conditions: frequency 40khz, power 200W, 70% gear). The material was suction filtered, and the filter residue was ultrasonically leached with 10L acetone for 60 minutes, and then suction filtered. The filtrates were combined, and the filtrate was concentrated in vacuo (30° C.) to obtain a crude extract of sulforaphane. Use silica gel column chromatography, sili...

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Abstract

The invention discloses a method of extracting sulforaphen. The smashed seeds of west orchids are added with two to three times as much water, the pH value is regulated to 3.8 to 4.2, ascorbic acid is added, and hydrolysis lasts seven to nine hours under the temperature of 20 to 30 DEG C; after being refrigerated and dried, the raw solution is added with fifteen to twenty five times as much propanone, supersonic extraction lasts forty to eighty minutes, and pumping filtration or double-gauze filtration follows; under the temperature of 30 to 50 DEG C, filtrate undergoes vacuum concentration in order to obtain crude sulforaphen; the crude sulforaphen is chromatographed by a silica gel column and gradiently eluted by n-hexane acetone solution, the thin-layer chromatography or HPLC tests and determines fractions containing the sulforaphen, and the crude sulforaphen undergoes vacuum concentration under the temperature of 30 to 50 DEG C; after being resolved in a small amount of acetone, the crude sulforaphen is chromatographed by a Sephadex LH-20 column, the acetone is used as eluent, the thin-layer chromatography or HPLC tests, determines and collects liquor containing the sulforaphen, and by vacuum concentration under the temperature of 30 to 50 DEG C, a sulforaphen product with eighty percent purity is produced. The method of the invention can also be used to extract the sulforaphen from the seeds of radishes, cabbages and mustards.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and relates to a method for extracting active ingredients in natural products, in particular to a method for extracting sulforaphane from plant seeds, especially the seeds of cruciferous vegetables such as broccoli, radish, cabbage and mustard Method for extracting sulforaphane from Background technique [0002] According to the statistics of the World Health Organization, the number of people who die from cancer every year in the world is about 7 million, and 1 million people in my country also lose their lives because of it. In the next 20 years, the number of new cancer patients worldwide will increase from 10 million to 50 million per year, and the number of cancer deaths will increase from 6 million to 10 million per year. This is a very heavy set of data, but until now, we have not found a way to conquer cancer. Therefore, studying and controlling tumors is a major topic in the field...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C331/20A61K36/31
Inventor 王向阳沈莲清苏光耀
Owner ZHEJIANG GONGSHANG UNIVERSITY
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