Preparation method and application of phellinus igniarius phenol A

The isolation of mulberry chlorophenol from mulberry yellow through multi-stage extraction and column chromatography separation technology solves the problem of difficult to effectively utilize mulberry yellow polyphenol compounds in the prior art, achieves a significant inhibitory effect on a variety of tumor cells, and preliminarily verifies its anti-cancer activity and mechanism.

CN119661552AInactive Publication Date: 2025-03-21ANHUI SENJINGGU PHARM CO LTD +1
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Patent Information

Application Number
CN202411840190.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-13
Publication Date
2025-03-21
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The prior art is difficult to effectively utilize polyphenol compounds in mulberry yellow as antitumor drugs, especially in inhibiting the Wnt/β-catenin signaling pathway.

Method used

Through the multi-stage extraction method of aqueous ethanol solution and ethyl acetate, combined with silica gel column chromatography, Sephadex LH-20 and ODS semi-preparation column separation and purification of moltenol from moltenol was successfully isolated and purified, and its inhibitory activity on a variety of tumor cells was verified.

Benefits of technology

Mulberry mothin has significant inhibitory activity on all six tumor cells in vitro, especially the most significant inhibitory effect on liver cancer cells. Its effect of downregulating the Wnt/β-catenin pathway was verified through in vivo tumor transplantation experiments and western blots of nude mice, and its anti-cancer activity and mechanism were preliminarily verified.

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Abstract

The invention relates to the technical field of fungus active ingredient extraction and medical application, and particularly provides a preparation method and application of phellinus igniarius phenol A. The preparation method comprises the following steps: extracting phellinus igniarius with an ethanol water solution and ethyl acetate in sequence to obtain an extract; the extract is subjected to silica gel column chromatography, Sephadex LH-20 (MeOH) column chromatography and ODS semi-preparative column separation in sequence, and the phellinus igniarius phenol A is obtained. In-vivo animal experiments prove that the phellinus igniarius phenol A has certain in-vitro inhibitory activity on six tumor cells, namely NCI-H460, MKN-45, 22Rv1, MCF7, SiHa and Hep 3B2.1-7, and particularly has the most remarkable in-vitro inhibitory effect on liver cancer Hep3B2.1-7 cells.
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