543 results about "Phellinus igniarius" patented technology

The invention relates to the technical field of biology; in order to solve the deficiency of wild phellinus igniarius resources at present, and the problems of insufficient medium nutrition supply, small sporophore growth, and easy undesired microbe infection of bag cultivation in artificial cultivation, the invention provides a phellinus igniarius bag cultivation medium and a method for cultivating phellinus igniarius sporophores by using the medium; the phellinus igniarius bag cultivation medium is prepared by mixing nutrients with water, packaging the mixture in bags, sterilizing the bags at a temperature of 110-135 DEG C, at a pressure of 0.05-0.25 MPa, for 30-180 min, wherein the mass ratio of the nutrients to water is 1:1.8-2.5. With the phellinus igniarius bag cultivation medium formula of the invention, nutrients absorbed during phellinus igniarius sporophore growth are provided, and the cultivated phellinus igniarius sporophores are similar to wild phellinus igniarius with stable quality; and the cultivation mode of the invention is simple, and can be used for large scale cultivation of phellinus igniarius sporophores with medicinal value.

The invention relates to a production method of novel edible and medicinal fungi solid fermented powder, which is characterized in that: 100 percent of edible and medicinal fungi sporophore (respectively Phellinus igniarius and lucid ganoderma) powder with 40 to 50 percent of moisture content is used as a culture medium, after being sterilized, strains such as Antrodia camphorata or lucid ganoderma, Hericium erinaceus, Agaricus blazei Murill, Cordyceps militaris, Phellinus igniarius, aweto and the like are inoculated to the culture medium, and after being cultured, relevant novel Antrodia camphorata or lucid ganoderma, Hericium erinaceus, Agaricus blazei Murill, Cordyceps militaris, Phellinus igniarius and aweto solid fermented powder can be obtained. The fermented powder can be made into capsules, orally-taken liquid and beverage and can be used as natural functional additives for other food. Compared with other composite compounded edible and medicinal fungi, the flow by utilizing fundamental medium to culture edible fungi mycelium and the extraction flow can be directly saved, the process is simple and is easy to operate, and no additive is added; and the fermented powder contains all active ingredients of the edible and medicinal fungi mycelium and sporophore, has the advantages of high content of active ingredients and the like, and is organic combination of different or homogenous medicinal and edible fungi sporophore and mycelium.

The invention discloses a large-scale artificial cultivation method for phellinus igniarius and mainly solves the problem that an existing method can not cultivate sporocarps rapidly in a large-scale mode. The method comprises the following steps: 1, obtaining protospecies, and inoculating the protospecies into a cultivating bag of a phellinus igniarius large-scale artificial medium for cultivation; 2, when phellinus igniarius hyphae overgrow the cultivating bag and the hyphae are deep yellow, or protruding nodular phellinus igniarius primordium starts to appear in the cultivating bag, transferring the cultivating bag full of the hyphae into a fruiting house for conducting fruiting cultivation. The cultivation environment conditions inside the fruiting house is that the relative air humidity ranges from 85% to 98%, and the temperature ranges from 22 DEG C to 28 DEG C. The method has the advantages that the phellinus igniarius sporocarps can be cultivated rapidly in a large-scale mode.

The invention pertains to the field of extracting active ingredients of natural products and particularly relates to an extracting method and a purifying method of crude phellinus igniarius polysaccharides. The technical problems needed to be solved are to disclose a method for extracting the extract of crude phellinus igniarius polysaccharides and an efficient method for preparing phellinus igniarius polysaccharides through countercurrent chromatography. An ultrasonic extraction is adopted by the invention to extract crude phellinus igniarius polysaccharides from phellinus igniarius fruit bodies; the high-speed countercurrent chromatography is adopted to further purify the crude phellinus igniarius polysaccharides. The extracting method and the purifying method of the invention have high yield of crude phellinus igniarius polysaccharides, and can take up 6 percent of crude drugs; the purity of the further purified phellinus igniarius polysaccharides is greatly improved to be about 75 percent.

The invention provides a phellinus igniarius juice health-care food and a preparation method thereof. The preparation method comprises the following steps of firstly, preparing a liquid fermentation medium; secondly, performing liquid fermentation on a phellinus igniarius strain; thirdly, collecting fermentation liquor obtained by fermenting phellinus igniarius liquid, and performing clarification or homogenization treatment on the fermentation liquor; fourthly, performing sterilizing, filling or spray drying treatment on the clarified or homogenized fermentation liquor. According to the phellinus igniarius juice health-care food and the preparation method thereof provided by the invention, juice is utilized to directly serve as a carbon source for fermenting phellinus igniarius, and nutritional ingredients and health-care ingredients obtained by fermenting the phellinus igniarius directly infiltrate into the juice to prepare the healthy food with the function of enhancing the physiological actions such as immunity and the like. The phellinus igniarius juice health-care food and the preparation method thereof have the advantages of simple technical operation, safety, reliability and suitability for industrialized large-scale production.

The invention relates to a GABA-rich mulberry leaf enzyme beverage stock solution as well as a preparation method and application thereof. The preparation method comprises the following preparation steps of completely immersing mulberry leaves in water; then putting a glutamic acid solution for soaking treatment; after treatment under the conditions of ultrasonic waves, performing microwave treatment, taking out the treated mulberry leaves, cutting the taken mulberry leaves into fine mulberry leaves, withering the fine mulberry leaves, rolling the withered mulberry leaves, placing the rolled mulberry leaves in an oven for drying, and crushing the dried mulberry leaves with a crusher for standby application; mixing the crushed mulberry leaves and PDA liquid culture media to obtain mixed culture media; inoculating strains of glossy ganoderma bacteria or phellinus igniarius bacteria to the obtained mixed culture media for preliminary fermentation; adding cane sugar, fruits and vegetables, and inoculating microzymes for fermentation for the second time; and then inoculating black tea bacteria for comprehensive fermentation, and performing filtration on fermentation liquor so as to obtain the mulberry leaf enzyme beverage stock solution rich in the GABA. The obtained beverage not only is delicious but also contains a higher content of the GABA, so that enzymes have the health-care effects of the GABA on reducing blood pressure, soothing the nerves and treating senile diseases.

The invention discloses a Phellinus igniarius (L.ex Fr.) cultivating seed preparation method. The Phellinus igniarius (L.ex Fr.) cultivating seed preparation method includes firstly, cutting mulberry twigs into small blocks, evenly mixing the small blocks, mulberry wood bits, wheat bran and gypsum, bagging a mixture, sterilizing, inoculating a Phellinus igniarius strain and culturing for 25-35 days so as to obtain an activated Phellinus igniarius strain; secondly, preparing a Phellinus igniarius cultivating culture medium from mulberry wood bits, wheat bran, maize meal, monopotassium phosphate, magnesium sulfate, gypsum and white sugar, bagging, sterilizing and inoculating the activated Phellinus igniarius strain; thirdly, culturing in a dark but ventilative culture room at a room temperature of 22-28 DEG C and at a relative air humidity of 60-75%; finally, taking Phellinus igniarius (L.ex Fr.) out after culturing for 60-80 days. The Phellinus igniarius (L.ex Fr.) cultivating seed preparation method has the advantages that a growth process of the Phellinus igniarius (L.ex Fr.) in a natural world is simulated, artificial cultivating of the Phellinus igniarius (L.ex Fr.) can be achieved truly, the Phellinus igniarius (L.ex Fr.) with hyphae growing fast is capable of spreading culture bags fast and yields a great number of fruiting bodies, every fruiting body is heavy and attractive in shape, proneness to growing of sundry fungi is avoided, bag discharge is fast and simple, and the Phellinus igniarius (L.ex Fr.) is good in commercial quality; the preparation method is simple in fruiting mode and suitable for large-scale production, thereby being worthy of popularization.

A method for culturing the Se-enriched sporophores of phellinus igniarius and extracting the selenopolyose from them includes such steps as preparing the mother culture medium from 13 raw materials including potato, agar, glucose, bean powder, etc, preparing stock culture medium from 14 raw materials including mulberry wood chip, wheat bran, corn flour, bean powder, etc, inoculating, culturing stock, culturing three-class spawn, preparing mycoplasm, and extracting selenopolyose. It can be used for preventing and treating cancer.

The invention relates to mythic fungus and phellinus igniarius health preserving tea and a preparation method thereof, and belongs to the technical field of tea. The technical problem needed to be solved is to provide the fungus and phellinus igniarius health preserving tea which contains a plurality of types of nutrient. According to the technical proposal used to solve the technical problem, the components are mythic fungus, green tea, phellinus igniarius, kudzuvine root, mulberry leaf and ginseng leaf; the preparation method comprises a crushing and a sieving procedures. The invention is applied to tea drink. The mythic fungus and phellinus igniarius health preserving tea has the advantages that the tea is rich in a plurality of types of sugar, amino acid and trace elements, can effectively clear oxygen free radical, strengthen the immunity of human body, can protect liver, detoxifcate and delay aging, and has treatment effect in low- and high-blood pressure diseases, cardio-cerebral-vascular diseases, diabetes, cancer, etc.

The invention belongs to the field of biotechnology, and particularly relates to a phellinus igniarius bacterial strain and application of the phellinus igniarius bacterial strain. The phellinus igniarius bacterial strain is relatively simple in cultivation and management and relatively high in yield, and large-area greenhouse cultivation can be carried out. The phellinus igniarius bacterial strain is collected in the China General Microbiological Culture Collection Center (CGMCC) in March 7, 2014, and the serial number of the phellinus igniarius bacterial strain is CGMCC No.8854. According to the phellinus igniarius bacterial strain, the manual bag cultivation process is adopted; a lot of cultivation practice proves that real manual cultivation of phellinus igniarius can be realized, and moreover, the size of a single sporocarp can be manually controlled according to the size of an opening. Since manual bag cultivation of the phellinus igniarius is realized, wild and scarce resources and long regeneration resources are manually regenerated in a short time.

The invention provides a method of extracting Phellinus igniarius polysaccharides, and the method is characterized by comprising: crushing the dried Phellinus igniarius with a grinder, sieving with a 40-mesh screen, placing into a high-energy nano impact mill for pulverization, and obtaining Phellinus igniarius nano powder with an average particle size of 100-500 nm; and then, extracting for 30 ~ 90min at a temperature of 80-100 DEG C by using water with a weight of 15-20 times of that of the Phellinus igniarius, cooling to the room temperature and filtering to give a Phellinus igniarius polysaccharide solution. According to the method of extracting Phellinus igniarius polysaccharides of the invention, the extraction temperature is low and the extraction time is short, thereby preventing damage of the Phellinus igniarius polysaccharide molecular structure, and maintaining the biological activity of polysaccharides.

The invention discloses a solid fermentation medium for phellinus igniarius mycelium and a solid fermentation cultural method for the phellinus igniarius mycelium, belonging to the technical field of phellinus igniarius artificial culture. Corn flour, wheat bran and the like are used as materials for preparing culture medium which can be used for the solid fermentation culture medium for the phellinus igniarius mycelium. Compared with the current phellinus igniarius mycelium liquid fermentation and phellinus igniarius solid cultural method, the cultural method can acquire much phellinus igniarius mycelium while economical and convenient to operate; moreover, the content of polysaccharide and flavone is higher than that of crude polysaccharides and flavone in each gram of sporophore.

The invention provides a method for applying plant oil to submerged culture of a medicinal fungus phellinus igniarius and improving phellinus igniarius hypha amount. A phellinus igniarius fermentation medium is prepared according to a technique disclosed in a patent with the patent application number of 200810070224, the initial pH of the medium is controlled to be 5.5-7.5, the sterilization temperature is 115 to 121 DEG C, and the sterilization time is 30 minutes. In the initial stage of the fermentation, different types of plant oil with the concentration of 0.5 to 5 mass percent are added, including soybean oil, olive oil, rap oil and the like, a shake flask fermentation or fermentation tank fermentation process is adopted, the inoculum size is 5 to 15 percent, and the culture temperature is 24 to 28 DEG C. The method has the advantages of simple operation process and low fermentation production cost, can be used for large-scale submerged culture production process of the phellinus igniarius, and also can be used for the liquid fermentation production process of other medicinal fugi, such as lucid ganoderma, agaricus blazei and lentinus edodes.

The invention discloses a separating culturing method of Chinese wild morin mushroom strain in the bacterial separating culture technical domain of fungi, which comprises the following steps: (1) making the culture medium of test tube oblique plane according to the proportion and dispense with 220-260g potato, 18-25g glucose, 0. 8-1. 2g potassium biphosphate, 0. 4-0. 6g magnesium sulfate, 18-12mg vitamin B, 80-100g mulberry branch, 18-20g agar, 1000mL water with pH value at 6-7. 0; (2) separating to culture: disposing the solid of morin; separating morin; culturing mother seed. The invention can accelerate the growth and breeding of hypha effectively to improve the activity of the strain, which realizes the high production of hypha fermented by morin liquor to reach 8. 83g dried hypha in the 1000ml liquor evenly.

The invention discloses a phellinus igniarius strain and a culturing method for the same. The strain is classified and named as Inonotus sanghuang, and is preserved with a preservation number CGMCC No.6395. The culturing method for the strain comprises the following steps of 1, preparing a culture medium; 2, performing inoculation, namely extracting a phellinus igniarius strain block in n aseptic environment by virtue of an inoculation tool, transferring the phellinus igniarius strain block into the middle part of a test tube culture medium, and plugging the test tube culture medium with cotton seeds; 3, performing strain culturing, namely transferring a test tube inoculated in step 2 into a constant temperature incubator, and performing constant temperature culturing for 10 to 15 days at 25 to 35 DEG C in a darkroom. According to the phellinus igniarius strain and the culturing method for the same, the phellinus igniarius strain cultured by the method is discovered and separated from a phellinus igniarius strain SH21022 (Inonotus sanghuang Sheng H.Wu, T.Hatt.&Y.C.Dai, sp,nov) on mulberries, and grows vigorously and rapidly, and large-scale mycelium fermentation production, active ingredient extraction and healthcare product or medicament development can be realized to meet market requirements.

The invention relates to a domestication, cultivation, concentration method of selenium morin bacterial. Culture medium is manufactured by 78g mulberry chip, 20g bran, 1g magnesium sulfate, 1g gypsum, 40mg sodiun selenite per Kg material according to water and material with 1:1. 2-1. 3. The method ascertains concentration of selenium according to the enriched ability of the morin bacterial; the content of selenium adds step by step fit for bacterial until the hypha changes more white, strong and the growth isn't limited; improves the selenium content of fruiting body. The invention improves the effect of beauty treatment, health care and medical treatment, and the value in use of selenium morin bacterial is very high.

The invention discloses a soybean, corn and phellinus igniarius healthy food, and a preparation method thereof. The preparation method comprises the steps of carrying out liquid fermentation by using phellinus igniarius as a starting strain and foods of the soybean and the corn as main basic materials to obtain phellinus igniarius liquid mycelium pellets; inoculating the phellinus igniarius liquid mycelium pellets on a soybean and corn solid medium for fermental cultivation to obtain a lot of soybean, corn and phellinus igniarius mycoplasmas; and finally smashing the soybean, corn and phellinus igniarius mycoplasmas to obtain a healthy food. A product provided by the invention has special fragrance and includes active ingredients of the phellinus igniarius, the soybean and the corn. A process is simple and stable; and a production period is short. The healthy food and the preparation method have great significance for protecting wild phellinus igniarius sources, relieving disadvantages of phellinus igniarius fruiting bodies, reducing cost and developing phellinus igniarius health-care foods industrially.

the invention discloses a morin 4.6302 strain fungal polysaccharide, which is cultivated by 60-80% rice bran, 8-12% corn powder, 8-12% soy powder, 0.8-2.2% glucose, 0.8-2.2% beef grease, 0.04-0.06% MnSO4 and 80-120% water at 22-30 deg.c for 10-20 days.

The invention relates to a cultivation method for fungi, in particular to a cultivation method for a phellinus igniarius manual fungus stick. The cultivation method comprises the following steps: 1) grafting strain, separated from wilding phellinus igniarius entity and conducted by purification, on parent strain culture medium for cultivation to obtain phellinus igniarius parent strain; grafting the phellinus igniarius parent strain on protospecies culture medium for cultivation to obtain phellinus igniarius protospecies; grafting the phellinus igniarius protospecies on cultigen culture medium for cultivation to obtain phellinus igniarius cultigen; 2) grafting the phellinus igniarius cultigen in the fungus stick to conduct ganoderma cultivation; 3) conducting ganoderma management. Testified by massive cultivation practice, the manual bag-cultivating technology can realize true phellinus igniarius manual cultivation; the size of individual child entity can be controlled manually according to the size of the opening; manual bag-cultivating of the phellinus igniarius manually regenerates the resources which are wilding, scarce, and long in regeneration in short term.

The invention relates to a cultivation method of mushrooms, in particular to a manual bag cultivation process for phellinus igniarius. The process comprises the following steps: (1) purifying strains separated from wild phellinus igniarius bodies, and inoculating the purified strains into a mother culture medium for culturing to obtain a phellinus igniarius mother culture, inoculating the phellinus igniarius mother culture onto a stock culture medium for culturing to obtain a phellinus igniarius stock culture, and inoculating the phellinus igniarius stock culture onto a cultivated species culture medium for culturing to obtain phellinus igniarius cultivating species; (2) inoculating the phellinus igniarius cultivating species into bacteria sticks for performing ganoderma emergence culturing; (3) performing ganoderma emergence management. Proved by a large amount of culturing practice, the manual bag cultivation process has the advantages that true manual cultivation of phellinus igniarius can be realized, and the size of a single fruit body can be controlled manually according to opening sizes; through manual bag cultivation of the phellinus igniarius, wild rare resources and hardly-regenerative resources are manually regenerated in a short time.

The invention relates to a phellinus igniarius bacterial strain liquid fermenting culture medium and a method for fermenting and producing phellinus linteus polysaccharides. The culture medium has a prescription shown as follows: 20 to 30g/L of glucose, 2 to 6g/L of yeast powder, 1 to 3g/L of KH2PO4, 0.3 to 0.7g/L of MgSO4.7H2O, 110mg/L of VB, and 1L of water; a pH is under a natural condition; a fermenting condition is that a temperature is at 28 to 32 DEG C, a rocking speed is at 140 to 180rpm, inoculation amount is 20 to 30%, cultivation is carried out for 9 to 11 days; the method comprises the following steps of: centrifuging, collecting fermenting liquid, washing via distilled water, precipitating to obtain a mycelium, then extracting polysaccharide inside or outside cells. With the adoption of the phellinus igniarius bacterial strain liquid fermenting culture medium and the method for fermenting and producing the phellinus linteus polysaccharides provided by the invention, yield of the mycelium of phellinus igniarius and content of the polysaccharide inside or outside the cells can be obviously increased; moreover, yields of the phellinus linteus polysaccharides is 6.34% higher than that of polysaccharide extracted by a common hot water extracting method. An animal test proves that extracted phellinus linteus polysaccharides have obvious biological activity such as tumour resistance, oxidization resistance, and improvement of immunity.

The invention provides a synthetic medium for culturing a phellinus igniarius liquid. The synthetic medium takes glucose as a carbon source and 20 kinds of amino acid as a nitrogen source, and consists of inorganic salt and vitamin. The ratio of carbon to nitrogen is designed as 50 to 1; and the specific concentration g/l is as follows: the glucose: 30 to 80 grams; glutamic acid: 0.1 to 1.5 grams; glutamine: 0.1 to 1.5 grams; aspartic acid: 0.1 to 1.5 grams; asparagines: 0.1 to 1.5 grams; glutamic acid: 0.01 to 0.1 gram; tryptophan: 0.01 to 0.1 gram; valine: 0.01 to 0.1 gram; alanine: 0.01 to 0.1 gram; etc. During the whole culture process of the synthetic medium, phellinus igniarius mycelium is quick in growth; the yield of the phellinus igniarius mycelium can reach 20 grams per liter to the highest degree; simultaneously no agricultural and sideline product is introduced as the carbon source and the nitrogen source; substances such as proteins in the medium, medium compositions and so on are not carried; phellinus igniarius exopolysaccharide produced through fermentation has high purity and stable quality; and the yield of phellinus igniarius polysaccharide can reach 0.198 gram per liter to the highest degree. The synthetic medium has the advantages of clear compositions, stable quality and so on, and can be used for mass culture of the phellinus igniarius liquid and research of the regulation and control rule of polysaccharide anabolism.