212results about How to "Good anti-tumor" patented technology

The present invention provides a single-stranded circular RNA and DNA, and a preparation method and application thereof, the single-stranded circular RNA and DNA can adsorb a single-stranded circularRNA or a single-stranded circular DNA of miRNA, thereby overcoming the disadvantage that a traditional single-stranded RNA is easily degraded and has low efficiency, and can release tumor suppressor gene ''co-silencing'' caused by the miRNA. The single-stranded circular RNA or DNA prepared by the method can specifically adsorb the target miRNA so as to inhibit the proliferation, migration and invasion of tumor cells and promote the apoptosis of the tumor cells, can regulate the proportion of immune cells, has good anti-tumor and immune regulation effects, and is expected to be developed as a therapeutic drug for tumors or immune diseases.

The invention relates to the technical field of crude drug, more specifically to a linaloe leaf extract. The invention also discloses its preparation and use in pharmaceuticals and health products.

The invention relates to a nutritious and healthy unit edible vegetable oil, which is characterized by adopting unit vegetable oil and fat rich in Omega-3 type polyunsaturated fatty acid and Alpha-linolenic acid as main materials, and adding algae oil rich in EPA and DHA. In the unit edible vegetable oil, the content of the algae oil is 0.05 percent to 5 percent of the weight of edible oil, and the content of the Omega-3 type polyunsaturated fatty acid is 8 percent to 70 percent of the weight of edible oil; and simultaneously the edible oil also adds an oxidizing agent as an additive. The nutritious and healthy unit edible vegetable oil has the advantages that: 1. by a reasonable formula of the edible oil, increasing the intake of the Omega-3 type polyunsaturated fatty acid, and further adding an effective path; 2. solving the defects of bad flavor and mouthfeel and poor stability of the edible oil added with algae oil; 3. adding a novel brain-strengthening health-care product, thus meeting the requirements of people for health diet; and 4. having strong market prospect.

The invention relates to a method for preparing a biological polysaccharide self-assembly modificatory chitosan antibacterial biological material, which is characterized by comprising the following steps: (1) preparing a lentinan derivant, i.e. lentinan sulfate; (2) preparing a chitosan membrane base material; (3) preparing a lentinan sulfate solution and a chitosan solution; and (4) carrying outlayer-by-layer self-assembly of the lentinan sulfate and the chitosan on the surface of the base material: respectively carrying out alternative self-assembly on the chitosan membrane base material in the lentinan sulfate solution and the chitosan solution for 5 times to obtain a surface layer-by-layer self-assembly modificatory chitosan mebrane containing 5 bilayers with the outermost layer of the lentinan sulfate, washing and drying to obtain the biological polysaccharide self-assembly modificatory chitosan antibacterial biological material. The chitosan antibacterial biological material prepared by the method has excellent antibacterial activity; and the method has simple preparation process, easy control, mild preparation conditions and low cost.

The invention relates to a phellinus igniarius bacterial strain liquid fermenting culture medium and a method for fermenting and producing phellinus linteus polysaccharides. The culture medium has a prescription shown as follows: 20 to 30g/L of glucose, 2 to 6g/L of yeast powder, 1 to 3g/L of KH2PO4, 0.3 to 0.7g/L of MgSO4.7H2O, 110mg/L of VB, and 1L of water; a pH is under a natural condition; a fermenting condition is that a temperature is at 28 to 32 DEG C, a rocking speed is at 140 to 180rpm, inoculation amount is 20 to 30%, cultivation is carried out for 9 to 11 days; the method comprises the following steps of: centrifuging, collecting fermenting liquid, washing via distilled water, precipitating to obtain a mycelium, then extracting polysaccharide inside or outside cells. With the adoption of the phellinus igniarius bacterial strain liquid fermenting culture medium and the method for fermenting and producing the phellinus linteus polysaccharides provided by the invention, yield of the mycelium of phellinus igniarius and content of the polysaccharide inside or outside the cells can be obviously increased; moreover, yields of the phellinus linteus polysaccharides is 6.34% higher than that of polysaccharide extracted by a common hot water extracting method. An animal test proves that extracted phellinus linteus polysaccharides have obvious biological activity such as tumour resistance, oxidization resistance, and improvement of immunity.

The invention discloses an enzymatic preparation method of low molecular pectin. The method comprises the following steps: carrying out enzymolysis and de-esterification on high molecular high ester pectin by virtue of one or more of pectinase, cellulase, galacturonase and pectinesterase; and carrying out alkali liquor neutralization, filtration and drying and grinding on the product to obtain a pectin product with targeted molecular weight and degree of esterification. According to the method disclosed by the invention, the high molecular high ester pectin is hydrolyzed by adopting a multienzyme compound hydrolyzing technology so as to extract pectin with different molecular weights and degrees of esterification, high content of galacturonic acid and excellent taste from natural pectin. The low molecular pectin powder prepared by the preparation method meets the USP30 standard, various chance elements in the prior art are avoided, and the purity and success rate for extracting the low molecular pectin are improved. Meanwhile, the method is short in process flow, strong in capability of industrialization and suitable for being popularized on a large scale.

The invention belongs to the technical field of biological medicines and nanomedicine and relates to an RGD (Arginine-Glycine-Aspartic Acid) peptide modified double-layer medicine carrying cation nanometer particle. The inner core of the nanometer particle disclosed by the invention is a polylactic acid-hydroxyacetic acid PLGA medicine carrying layer; the outer layer of the nanometer particle is an RGD modified cation polymer layer which is carried with a medicine sensitizer. The nanometer particle can penetrate through blood brain barrier through receptor mediation and electrostatic adsorption action, can be gathered at the brain tumor tissue after entering the brain, can deeply penetrate through the solid parts of the tumor under the RGD mediation to specially combine with tumor new vessel endothelial cells and tumor cells and can enter the cells through endocytosis. The sensitizer dispersed at the outer layer is firstly released to play a role of reversing tumor drug resistance and the medicine dispersed at the inner layer is slowly released, so that the efficient, lasting and safe anti-tumor curative effect of the effect is brought into play.

The invention discloses a hangover alleviating and liver protecting functional fermented beverage and a preparation method thereof, and belongs to the field of microbial fermentation application. Thefermented beverage consists of the following raw materials: 5-10g of red dates, 10-15g of kudzuvine root powder and the balance of water, wherein probiotics used for fermentation of the hangover alleviating and liver protecting functional fermented beverage are one of lactobacillus plantarum or pediococcus acidilactici. According to the invention, polysaccharides, vitamins, cellulose, phenolic substances, flavonoid substances and the like in red dates and kudzuvine roots exert a maximum effect through probiotic fermentation; the fermented beverage not only preserves the nutritional value of natural plants, but also has the nutritional and health-care effects of probiotic fermentation products; and the fermented beverage is unique in taste and obvious in effect and has a good market prospect.

The invention relates to healthcare chickpea nutrition powder with an anti-fatigue effect. The healthcare chickpea nutrition powder is characterized by comprising the following raw materials in percentage by mass: 70%-85% of chickpea powder, 5%-10% of Chinese herbal medicine powder and 10%-20% of seasoning powder. The healthcare chickpea nutrition powder has a very good anti-fatigue effect, can be directly mixed with hot water for eating, also can be further processed into staple foods such as cakes, breads, biscuits and noodles for eating, easily integrates into daily life of people, and is low in price and easy to popularize; in addition, the seasoning powder such as coffee powder or fruit powder also can be added, so that the healthcare chickpea nutrition powder has different flavors and mouth feels, meets the requirements of different crowds and has a wide market prospect.

The invention discloses a preparation method of fermented rice bran feed and an application thereof. The preparation method of the fermented rice bran feed disclosed by the invention comprises the following steps: adding a complex microbial inoculant and a complex enzyme preparation into a fermentation raw material taking rice bran as a main component to obtain a fermentation system, and fermenting the fermentation system to obtain a fermentation product which is the fermented rice bran feed, wherein the active ingredients of the complex microbial inoculant are bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, lactobacillus casei, enterococcus faecalis, saccharomyces cerevisiae and candida utilis; the active ingredients of the complex enzyme preparation are cellulase, acid protease, amylase, beta-mannase, xylanase and beta-glucanase. The shelf life of the obtained fermented rice bran feed is prolonged, and the production performance of mammals and the yield and quality of produced milk can be further improved by utilizing the obtained rice bran fermented feed.

The invention discloses a method for producing glycyrrhizia polysaccharide by utilizing a tissue culture method. The method comprises the following steps of: (1) culturing and inducing liquorice aseptic seedling; (2) adopting an embryonic axis to induce liquorice callus under the liquorice aseptic seedling; (3) selecting the liquorice callus with high growth speed, loose texture and light yellow color, crushing the liquorice callus with a pair of forceps, transferring the crushed liquorice callus into a liquid culture medium for suspension culture and obtaining liquorice suspension cells; (4) culturing the liquorice suspension cells by using a bubble type reactor; and (5) extracting the glycyrrhizia polysaccharide from the liquorice suspension cells obtained in the step (4) to obtain the glycyrrhizia polysaccharide powder. The glycyrrhizia polysaccharide produced by the suspension cells is higher in content than the wild liquorice, has obvious oxidation resistance, virus resistance, tumor resistance and immunoregulatory activity, and has very strong immunoregulatory and anti-fatigue effects. The method is not limited by seasons or regions, is convenient, is easy to operate, and has a wide development prospect.

The invention provides a triple antigen and antibody complex for preventing and controlling porcine viral diarrhea and a preparation method of the triple antigen and antibody complex. The triple antigen and antibody complex comprises an antigen and an antibody, wherein the antigen is a triple virus liquid obtained by mixing porcine transmissible gastroenteritis viruses, porcine epidemic diarrhea viruses and porcine rotavirus, and the antibody is a triple egg yolk antibody prepared on the basis of the antigen. The triple antigen and antibody complex for preventing and controlling porcine viral diarrhea is convenient and quick to use and capable of safely and effectively preventing and controlling the porcine viral diarrhea. The triple antigen and antibody complex can effectively reduce the body immunological stress response, increases the protection production speed, rapidly increases the body antibody titer and is prevented from the virus diffusion risk in production and use processes.

A health-care natto beverage in the form of instant particles with unique taste and rich nutrients is prepared from natto powder, milk powder, malt dextrin, phytolipin dust and additives.

The invention discloses eyesight-improving spleen-tonifying pearl Poria honey. The eyesight-improving spleen-tonifying pearl Poria honey is prepared by using the following raw materials in parts by weight: 6 to 7 parts of distillers' grains, 13 to 15 parts of clam meat mash, 3 to 4 parts of pearl powder, 14 to 15 parts of loquat, 4 to 5 parts of sesame, 2 to 2.3 parts of Poria, 1.7 to 2 parts of flatstem milkvetch seeds, 1.1 to 2 parts of radish seeds, 150 to 200 parts of honey, 60 to 70 parts of Eleutherococcus sessiliflorus fruits, 1.9 to 2 parts of sodium alginate, 1.7 to 1.8 parts of chitosan, 1.2 to 1.4 parts of calcium chloride and 50 to 60 parts of acetic acid with concentration of 1 to 2 percent. Eleutherococcus sessiliflorus anthocyanin prepared by using the Eleutherococcus sessiliflorus fruits has very good efficacies of resisting oxidation, mutation and tumors, protecting heart and blood vessels, improving eyesight, resisting radiation, improving immune and the like; since the anthocyanin is embedded in a closed capsule by adopting a microencapsulation technique, original bioactivity is better maintained, a certain slow release effect is achieved, the application range of Eleutherococcus sessiliflorus anthocyanin is expanded and the industrial chain of Eleutherococcus sessiliflorus is widened.

The invention relates to the field of medicine and discloses a microcrystal catechin and a preparation method thereof. The microcrystal catechin is characterized by spectral signatures of x-ray powder diffraction which uses Cu-K alpha radiation and is expressed at the angle 2 theta. The preparation method comprises the five procedures of steaming, membrane technology, organic solvent extraction, solvent recovery, and concentrating and drying. The microcrystal catechin has stable quality, long shelf life, good effects in treating hyperlipaemia and cardiovascular and cerebrovascular diseases, excellent oxidation resisting property and the excellent functions of diminishing inflammations, killing bacteria, clearing radicals, resisting tumors, resisting mutations, lowering blood fat and preventing atherosis. The microcrystal catechin is a valuable nature drug and has a wide clinic application prospect.

The invention belongs to the field of composite materials, and concretely relates to a nano-hydroxyapatite/carboxymethyl chitosan/poly(lactic-co-glycolic acid) micro-nano hybrid drug-loaded scaffold and a bionic preparation method thereof. The three-dimensional bionic hybrid drug-loaded scaffold is prepared through combining solvent evaporation and freeze drying technologies by using a high speed emulsifying machine via adopting nano-hydroxyapatite and carboxymethyl chitosan as a water phase and an emulsion continuous phase, adopting a methylene chloride solution of poly(lactic-co-glycolic acid) as an oil phase and an emulsion dispersion phase, using glutaraldehyde as a cross-linking agent to immobilize the carboxymethyl chitosan in the continuous phase and adopting the main component icariin in a traditional Chinese medicine epimeddium as a carrier drug. The preparation method has the advantages of simple preparation process and mild reaction conditions, and the obtained scaffold overcomes the performance defects of single natural polymer materials or synthetic polymer materials, has the advantages of intercommunicated pores, uniform pore diameter, figurability, slow drug release, good bone bonding ability and good biocompatibility, and is expected to become a new osteoporosis treatment composite material.

The invention provides a chiral spiro [3,2'] pyrrolidine oxoindole skeleton compound, and the chiral spiro [3,2'] pyrrolidine oxoindole skeleton compound has good anti-inflammatory, antibacterial andanti-tumor biological activity; meanwhile, the skeleton compound also contains a prolinamide skeleton structure, is easy to modify, and is a potential chiral ligand and chiral catalyst. The inventionalso provides a preparation method of the chiral spiro [3,2'] pyrrolidine oxoindole skeleton compound, wherein the method can simultaneously meet the requirements of short synthesis route, cheap and easily available raw materials, strong practicability, high reaction yield and the like, and has good popularization and application prospects. The invention further provides an intermediate for preparing the chiral spiro [3,2'] pyrrolidine oxoindole skeleton compound and application of the chiral spiro [3,2'] pyrrolidine oxoindole skeleton compound.

The invention provides isolated culture and extraction of plant stem cells of tomato and a preparation method of oral liquids, beverages and white spirit. As stem cells from a plant cambium of tomato is in an undifferentiated state, during amplified culture cells are high in hereditary stability, strong in activity, great in harvest rate and the like. The oral liquids, the beverages and the plant stem cell wine prepared by ethanol ultrasonic extraction have better use to resist cellular oxidation, prevent differentiated cells from turning to tumor cells, and reduce blood fat, blood pressure, cholesterol and e blood sugar.

The invention discloses a bispecific chimeric antigen receptor targeting CD123 and NKG2D ligands and the application of the bispecific chimeric antigen receptor, and particularly discloses a bispecific chimeric antigen receptor (CAR) amino acid construct or a functional variant thereof, wherein the bispecific chimeric antigen receptor (CAR) amino acid construct can simultaneously expresses an anti-CD123 single-chain antibody and a natural NKG2D extracellular fragment and can target CD123 and NKG2DL; the bispecific chimeric antigen receptor has a parallel connection mode and a series connectionmode; and the invention discloses a CAR (chimeric antigen receptor), a construct structure thereof, a nucleotide sequence of the construct, a recombinant expression vector containing the nucleotide sequence and a construction mode and application of the corresponding expression vector. The CAR structure endows T cells with higher and lasting multiplication capacity and high anti-tumor capacity; and according to the bispecific chimeric antigen receptor, multiple infusions of single-target CAR-T cells can be avoided, so that not only is the harm to a patient reduced, but also the economic pressure of the patient can be reduced, and the bispecific chimeric antigen receptor has relatively great clinical research and application values.

The invention discloses a sesquiterpene quinone compound separated and purified from abelmoschus sagittifolius, as well as a preparation method and applications thereof, relating to the technical field of medicines. The new sesquiterpene quinone compound with antitumor activity is named as Acylhisbiscone B, with the molecular formula of C17H22O4 and the structural formula shown in the specification. According to the preparation method, the detection on the antitumor activity of the new compound is carried out by adopting an MTT method through combination of column chromatography and preparative liquid chromatography purification methods. In vitro experiment results show that the new sesquiterpene quinone compound has activity on significantly inhibiting the growth of Hela (human cervical carcinoma cells) and HepG-2 (human hepatoma carcinoma cells). The new sesquiterpene quinone compound can provide new chemical entity or lead compound for research of antitumor medicaments, and can also be used as food raw materials for preparation of health food.

The invention belongs to the technical field of food production, and particularly relates to a liver protecting formula food containing oligopeptides and a preparing method thereof. The liver protecting formula food is prepared from 25%-35% of the soy protein oligopeptides, 18%-22% of polysaccharopeptide, 25%-35% of the goat milk protein oligopeptides, 8%-13% of sodium alga acid, 3%-6% of taurine, 3%-5% of stachyose and 0.05%-0.1% of vitamin B-complexes. The formula is characterized in that the micro-molecule oligopeptides can be rapidly absorbed, nutritional ingredients required by the livers can be rapidly supplemented, liver nutrients can be intensified, injured liver cells can be assisted to be repaired, liver-cell regeneration can be promoted, organism immunity can be improved, the resistant capacity of the livers can be enhanced, absorbing of harmful elements in the body can be further inhibited, and alexipharmic burdens of the livers can be reduced; meanwhile, lipid and cholesterol in the body can be assisted to be removed, accumulation of fat in the livers can be prevented, chemical liver injuries such as fatty livers and liver cirrhosis can be effectively prevented, and the liver protecting formula food is safe and free of any toxic and side effect.

The invention discloses a traditional Chinese medicine composition for treating dermatoses and a preparation method thereof. The medicine is prepared from the following bulk drugs in parts by weight: 15-25 parts of cortex pseudolaricis, 15-25 parts of salicylic acid, 25-35 parts of fructus cnidii, and 25-35 parts of fructus kochiae. The preparation steps comprise the follows: firstly, selecting the bulk drugs in parts by weight and smashing the selected bulk drugs into fine particles; then mixing the bulk drugs which are smashed into fine particles with rice wine at 50+/-5 degrees according to a weight proportion of 100: (250-350) for soaking for 25-30 days; then filtering away the slag to obtain a liquid medicine. The traditional Chinese medicine composition for treating dermatoses has easy source of the constituents and is low in cost. The traditional Chinese medicine composition has fast effect and high curing rate for treating the dermatoses such as eczema, tinea pedis and neurodermatitis, and also can be used for controlling relapse of the dermatoses such as eczema, tinea pedis and neurodermatitis efficiently.