Method for producing glycyrrhizia polysaccharide by utilizing tissue culture method

A technology of licorice polysaccharides and tissue culture, applied in tissue culture, biochemical equipment and methods, microorganisms, etc., can solve the problems of lack of licorice resources, etc., and achieve broad development prospects, easy operation, and obvious anti-oxidation effect

Inactive Publication Date: 2012-07-04
TIANJIN UNIV
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Problems solved by technology

[0003] To address the problems in the prior art, the present invention provides a method for producing licorice polysaccharides by culturing licorice cells to solve the problem of lack of licorice resources at present

Method used

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Embodiment Construction

[0017] The present invention will be further described below in conjunction with specific examples.

[0018] The first step, the acquisition of licorice aseptic seedlings: select licorice seeds with full particles and dark green, and use 10% sodium hypochlorite to stir and sterilize the seeds on a magnetic stirrer with a rotating speed of 300r / min and a temperature of 25°C. Sterilize for 30 minutes, MS culture contains 3% sucrose, pH 5.9 before sterilization, inoculate in a 50ml Erlenmeyer flask containing MS solid medium, place in a light incubator at 25°C, and cultivate for 30 days to obtain licorice sterile seedlings .

[0019] The second step is the acquisition of licorice callus: the licorice callus is induced from the hypocotyls of the aseptic seedlings of licorice, and the growth state is stable after multiple selection and subculture. The subculture medium of callus was MS medium, supplemented with 2,4-D (1 mg·L -1 ), NAA (1mg·L -1 ), 6-BA (0.2mg·L -1 ), 3% sucrose...

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Abstract

The invention discloses a method for producing glycyrrhizia polysaccharide by utilizing a tissue culture method. The method comprises the following steps of: (1) culturing and inducing liquorice aseptic seedling; (2) adopting an embryonic axis to induce liquorice callus under the liquorice aseptic seedling; (3) selecting the liquorice callus with high growth speed, loose texture and light yellow color, crushing the liquorice callus with a pair of forceps, transferring the crushed liquorice callus into a liquid culture medium for suspension culture and obtaining liquorice suspension cells; (4) culturing the liquorice suspension cells by using a bubble type reactor; and (5) extracting the glycyrrhizia polysaccharide from the liquorice suspension cells obtained in the step (4) to obtain the glycyrrhizia polysaccharide powder. The glycyrrhizia polysaccharide produced by the suspension cells is higher in content than the wild liquorice, has obvious oxidation resistance, virus resistance, tumor resistance and immunoregulatory activity, and has very strong immunoregulatory and anti-fatigue effects. The method is not limited by seasons or regions, is convenient, is easy to operate, and has a wide development prospect.

Description

technical field [0001] The invention relates to a cell culture method, in particular to a method for producing licorice polysaccharide by licorice cell culture. Background technique [0002] As a traditional Chinese medicine, licorice has the reputation of "ten directions and nine herbs" since ancient times, and licorice polysaccharide is one of the three main active components in licorice. Glycyrrhiza licorice is a commonly used bulk medicinal material in my country, and it is a national second-class protected plant; it is a nationally controlled medicinal material. The active ingredients in licorice have strong biological activity, wide application, high price, and increasingly strong market demand. However, the germination rate of wild licorice seeds is low, and Predatory exploitation; the increasingly serious contradiction between supply and demand; serious deserts, the production of active ingredients of licorice by tissue culture technology has broad development prospec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12N5/02C12N5/04A61P39/06A61P31/12A61P35/00A61P37/02A61P39/00
Inventor 高文远郭松波满淑丽吕连媛刘辉董艳艳王娟
Owner TIANJIN UNIV
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