Chinese yam polysaccharides extract and preparation method and application thereof

A technology for yam polysaccharide and extract is applied in the processing, extraction and separation of natural products, and in the food field, and can solve the problems of not disclosing the content of yam polysaccharide, difficult to realize industrialized production, high solvent toxicity, etc., and achieve immunity enhancement and production process simplification. , The effect of high production safety

Active Publication Date: 2010-01-20
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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Problems solved by technology

This method is directly extracted with fresh yam, and the yield of crude polysaccharide in terms of fresh yam is only 0.2449% (the yield of crude polysaccharide in terms of dry yam is about 1.7143%), the yield is low, and the content of yam polysaccharide is not disclosed; Chloroform+n-butanol) deproteinization, acetone, anhydrous ether washing, solvent toxicity is big, inflammable and explosive, and cost is high; Use Sevage method to deproteinize, also remove the glycoprotein (complex polysaccharide) with biological activity at the same time; The method and process are complicated, and it is also difficult to realize industrial production

Method used

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  • Chinese yam polysaccharides extract and preparation method and application thereof

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Embodiment 1

[0032]The yam slices are crushed into yam powder of more than 200 meshes; 250L of 80% ethanol aqueous solution is added to a 500L reaction pot, and 50kg of yam powder is added after stirring, heated and refluxed for 2 hours at a temperature of 50°C; centrifuged, the sediment is stirred with 250L of water, Use a colloid mill to make yam slurry; use a pump to input the yam slurry into the extraction tank, add 500L of water, start stirring, heat and reflux for extraction for 3 hours at a temperature of 50°C; centrifuge the water extract, and concentrate the supernatant under reduced pressure at 60°C. Prepare a liquid extract with a Baume degree of 7; add 2 to 10 times the volume of 95% ethanol to the liquid extract for ethanol precipitation, and centrifuge the ethanol precipitation solution; add 2 to 10 times the weight of water to the sediment, stir and dissolve, and centrifuge; The supernatant was vacuum freeze-dried to obtain 6.4 kg of yam polysaccharide extract; the product yi...

example 2

[0037] The yam slices are crushed into yam powder of more than 200 mesh; 500L of 85% ethanol aqueous solution is added to a 1000L reaction pot, and 100kg of yam powder is added after stirring, heated and refluxed for 4 hours at a temperature of 40°C; centrifuged, the sediment is stirred with 500L of water, Use a colloid mill to make yam slurry; use a pump to input the yam slurry into the extraction tank, add 1000L of water, start stirring, heat and reflux for extraction for 4 hours at a temperature of 40°C; centrifuge the water extract, and concentrate the supernatant under reduced pressure at 70°C. Prepare a fluid extract with a Baume degree of 20; add 2 to 10 times the volume of 95% ethanol to the fluid extract for ethanol precipitation, and centrifuge the ethanol precipitation solution; add 2 to 10 times the weight of water to the sediment, stir and dissolve, and centrifuge; The supernatant was spray-dried to obtain 10.8 kg of yam polysaccharide extract; calculated as dry ya...

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Abstract

The invention discloses a Chinese yam polysaccharides extract and a preparation method and application thereof. The preparation method comprises the following steps: crushing Chinese yam slices, extracting by ethanol, separating by centrifugation, depositing dregs and adding water, stirring, grinding by a colloid grinder, extracting by water, separating by centrifugation, decompressing and concentrating supernatant to prepare liquid extract; depositing the liquid extract by the ethanol, separating by centrifugation, depositing dregs and adding water, stirring and dissolving, separating by centrifugation, and drying liquid extract to prepare the Chinese yam polysaccharides extract, wherein the yield is 10-19 percent. The Chinese yam polysaccharides extract does not contain starch, and the weight percentage content of the Chinese yam polysaccharides extract is 30-61 percent. The Chinese yam polysaccharides extract has the healthcare function of assisting to regulate the blood sugar level and enhancing the immunity, and can be used for preparing medicines or health care products of regulating the blood sugar level and enhancing the immunity.

Description

technical field [0001] The invention belongs to the technical field of processing, extraction and separation of food and natural products, and relates to a polysaccharide extract of food and natural medicine yam, its preparation method and application. Background technique [0002] Yam is the root tuber of Dioscorea opposite Thunb., a perennial twisting herb of Dioscoreaceae. Yam has the functions of invigorating the spleen, nourishing the lungs, strengthening the kidney, and benefiting the essence. "Shen Nong's Materia Medica" is listed as top grade. It is said that it "replenishes deficiency and wins in the main injury, eliminates cold and heat evil spirits, nourishes energy and strength, and grows muscles. Take it for a long time for hearing and eyesight, light body and not hungry and prolong life." "Compendium of Materia Medica" says: "Yam is warm in nature, sweet in taste, flat and non-toxic. Strengthen the spleen and stomach, benefit the lungs and kidneys, relieve dy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8945A61P3/10A61P37/04A23L1/30A23L33/105A23L33/125
Inventor 潘福生张宗和庞自洁黄嘉玲夏冰徐浩杨如同秦清赵爱明陶林
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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