The invention relates to plant cell microspheres and particularly discloses plant cell microspheres and use thereof. The plant cell microspheres are characterized by being processed and manufactured on the basis of plant protoplasts, plant cells and plant sporopollen or pollen, wherein the protoplast-derived plant cell microspheres contain matrix, matrix channels, middle cavities, internal spheres and external pendants; the cell-derived plant cell microspheres contain outer walls, matrix, matrix channels, middle cavities, internal spheres and external pendants; and the sporopollen-derived plant cell microspheres contain outer walls, inner walls, matrix, matrix channels, middle cavities, internal spheres and external pendants. The plant cell microspheres of the invention can conduct sustained-release, controlled-release and other physical and chemical functions, and can naturally degrade after being used.