1171 results about "Plant cell" patented technology

The invention relates to a gene GmHB1a participating in development of vascular bundles of soybean root nodules and application of the gene GmHB1a. Specifically, the invention relates to a separated gene participating in development of vascular bundles of soybean root nodules or regulation and control of the number of soybean nodules, and a coding region sequence of the gene is shown as SEQ ID NO: 1 or a homologous sequence of the gene. The invention also provides a method for regulating and controlling the nodulation number of the plant, which comprises the following steps: introducing the gene or a sequence for inhibiting the expression of the gene into a target plant cell or tissue to obtain a transgenic plant, the nodulation number of the transgenic plant is changed compared with that of the target plant, the plant is a leguminous plant, preferably soybean, and the nodulation number of the transgenic plant is changed. Optionally, the change in number of nodules is manifested as an increase in number of nodules and / or in number of single nodules, and optionally, the change in number of nodules is manifested as a decrease in number of nodules and / or in number of single nodules.

A resistance gene TaCAT to FCR of wheat and an application thereof are provided, which relates to the field of biological genetic engineering technologies. The nucleotide sequence of a genome where the TaCAT gene is located, the nucleotide sequence of a cDNA, the nucleotide sequence of a CDS and a coded protein sequence are respectively as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. An InDel marker is developed for identifying different allelotypes of the TaCAT gene. An application of the TaCAT gene for preparation of transgenic plant cells and plant breeding is provided. The molecular genetic basis of the resistance to FCR is disclosed, an important role is played in improving the resistance to FCR by utilizing a genetic engineering technology, and a new way is provided for breeding a new wheat variety with disease-resistance and safety.

The present invention discloses a multi-modal system for real-time plant hydration monitoring and irrigation management. The system comprises at least one acoustic sensor configured to emit controlled sound waves through plant tissues and detect corresponding vibrations and resonance frequencies using sensitive microphones or piezoelectric sensors to assess plant hydration. The system includes at least one bioelectrical sensor for monitoring bioelectrical signals. At least one nanotechnology-based sensor is configured to detect molecular changes in water content within plant cells through embedded or externally applied nanosensors. An aerial imaging device mounted on an unmanned aerial vehicle captures data related to canopy temperature, leaf color, and hydration indicators using infrared, multi-spectral, and thermal cameras. A central processing unit receives and analyzes data using predictive models, integrates the data via data fusion algorithm to generate a plant hydration profile, and controls water delivery through an irrigation management unit based on the hydration profile.

The invention discloses a preparation method of an anti-aging cosmetic containing exosome, and relates to the technical field of cosmetic preparation, the cosmetic is prepared from the following components in percentage by mass: 1.01%-5.05% of exosome wrapped by liposome, 0.5%-3% of an antioxidant, 1%-3% of a centella asiatica extract, 1%-3% of a rhodiola rosea extract, 10%-15% of a humectant and the balance of a basic matrix; wherein the exosome wrapped by the liposome is a mixture formed by mixing an exosome secreted by adipose-derived mesenchymal stem cells and an exosome secreted by plant cells according to a mass ratio of 1.5: 1.2; the plant is ginseng. According to the skin care product, the antioxidant, the centella asiatica extract and the exosome act together to improve the DPPH clearance rate of the skin care product, the skin brightness is improved, the roughness is reduced, and the excellent anti-aging performance is achieved. The lipid exosome-antioxidant-centella asiatica ternary system disclosed by the invention has a multi-target synergistic effect.

The invention relates to application of turmeric rhizome extract emulsifiable concentrate in preventing and treating corn leaf spot and tomato gray mold. Compared with the prior art, the turmeric rhizome extract missible oil disclosed by the invention contains an effective substance aromatic turmerone, and the aromatic turmerone can reduce the spore germination rate and sporulation quantity of pathogenic bacteria, increase the permeability of hypha cell membranes and cause overflow of contents, so that normal growth of hyphae is inhibited, and an antibacterial effect is achieved. Aromatic turmerone acts on plants, so that plant cells generate more polyphenol and flavonoid substances, and the immunity of the plants is improved. Pharmacodynamic test results in fields in two fields show that the 6.5% turmeric rhizome extract emulsifiable concentrate can be used for stably and effectively preventing and treating corn northern leaf blight and tomato gray mold after being diluted by 150-300 times, and also has a yield increasing effect.

The invention relates to a gene GmHB21 participating in development of vascular bundles of soybean root nodules and application of the gene GmHB21. Specifically, the invention relates to a separated gene participating in development of vascular bundles of soybean root nodules or regulation and control of the number of soybean nodules, and a coding region sequence of the gene is shown as SEQ ID NO: 1 or a homologous sequence of the gene. The invention also provides a method for regulating and controlling the nodulation number of the plant, the method comprises the following steps: introducing the gene or a sequence for inhibiting the expression of the gene into a target plant cell or tissue to obtain a transgenic plant, the nodulation number of the transgenic plant is changed compared with that of the target plant, and the plant is a leguminous plant, preferably soybean. In addition, the invention also provides application of the gene or a sequence for inhibiting expression of the gene in cultivation of leguminous plants with changed nodulation number, such as soybean.

A transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63717 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63717, and methods of using and detecting Gm_CSM63717 are also provided. Soybean plants containing the event Gm_CSM63717 exhibit tolerance to PPO inhibitors.

Provided herein, inter alia, is an engineered non-plant cell that produces a hyoscyamine alkaloid product, a precursor of a hyoscyamine alkaloid product, or a derivative of a hyoscyamine alkaloid product. Also described is a method for producing a scopolamine alkaloid, a precursor of a scopolamine alkaloid product, or a derivative of a scopolamine alkaloid product using a cell.

The invention discloses a targeted delivery method of a plant-derived biological regulator, and relates to the technical field of bioengineering, and the targeted delivery method comprises the following steps: in the application process of an electroporation technology, monitoring and acquiring cell membrane lipid change data information in real time through a biosensor technology; the method comprises the following steps: preprocessing original data acquired by a biosensor, and organizing and storing the preprocessed data according to rules to form a structured data set; the system can accurately control the state of the plant cell membrane by monitoring the lipid change of the cell membrane in real time and combining intelligent evaluation and dynamic adjustment of the electric field parameters. When membrane lipid is oversaturated, the electric field intensity and the pulse frequency are dynamically adjusted, membrane damage is reduced, cell integrity is protected, and meanwhile effective delivery of allogenic materials is ensured. According to the method, the stability and the accuracy of an electroporation technology are improved, the targeted delivery of the plant-derived biological regulator is optimized, and the application potential of the plant-derived biological regulator in the fields of agriculture and biomedicine is expanded.

Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming plants, plant tissues, plant parts, plant cells, and plant seeds with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.

The present disclosure provides genetically modified plants, plant cells, or plant tissues wherein the plants, plant cells, or plant tissues comprise an exogenous nucleic acid comprising a nucleotide sequence encoding a 30S ribosomal protein or a homolog thereof, wherein the 30S ribosomal protein or the homolog thereof is expressed in the plant, plant cell, or plant tissue. The current disclosure provides methods of improving drought tolerance and water loss in a plant, plant cell, or plant tissue, the methods comprising an exogenous nucleic acid sequence encoding a 30S ribosomal protein or a homolog thereof, wherein the 30S ribosomal protein or the homolog thereof is expressed in the plant, plant cell, or plant tissue. Additionally, the current disclosure is describes to genetically modified plants, plant cells, or plant tissues wherein the plants, plant cells, or plant tissues comprise an exogenous nucleic acid comprising a nucleotide sequence encoding a cation / H+ exchanger 20 (CHX20) protein or a homolog thereof, wherein the nucleotide sequence is operably linked to a heterologous promoter, and the CHX20 protein or homolog thereof is expressed in the plant, plant cell, or plant tissue. Finally, the current disclosure provides methods of improving drought tolerance and water loss in a plant, plant cell, or plant tissue, the methods comprising an exogenous nucleic acid sequence encoding a CHX20 or a homolog thereof, wherein the CHX20 or the homolog thereof is expressed in the plant, plant cell, or plant tissue.

The invention provides nucleic acids, polypeptides, transgenic plants, compositions and methods for conferring pesticidal activity (e.g., insecticidal activity) to bacteria, plants, plant cells, tissues and seeds. Nucleic acids encoding the insecticidal proteins can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. The recombinant organisms or compositions containing the recombinant organisms or insecticidal proteins or in combination with an appropriate agricultural carrier can be used to control an insect pest in various environments.

The present disclosure provides methods of regulating iron deficiency response in plants, modified plants and plant cells derived therefrom, in particular plants and plant cells with altered iron content, iron transport, iron sensing, or iron uptake.

The invention relates to the technical field of pasture planting, and discloses a treatment method for improving cold resistance of pennisetum purpureum, according to the treatment method, a 1000 mg / L chitosan solution is used as an exogenous substance to be applied to the pennisetum purpureum, and more preferably, the 1000 mg / L chitosan solution and charcoal are jointly used as the exogenous substance to be applied to the pennisetum purpureum. Chitosan is used as a biological stimulant to stimulate the generation of cold-resistant substances in plants and help to protect plant cells from being damaged under a low-temperature condition, the chitosan solution is used for treating the pennisetum purpureum, the optimal use concentration for improving the cold resistance of the pennisetum purpureum is determined through tests, and the cold resistance of the pennisetum purpureum is remarkably enhanced. According to the method, the biochar is applied on the basis of applying the chitosan solution, so that the cold resistance of the pennisetum purpureum is further improved.

The invention discloses a cobalt nanoparticle / nitrogen-doped carbon composite material and a preparation method and application thereof, and belongs to the technical field of photocatalytic degradation materials.The preparation method comprises the steps that turnip strips are soaked in a solution of soluble Co < 2 + > salt and a nitrogen source, freeze drying and grinding are conducted, and a precursor of the cobalt / nitrogen-doped carbon composite material is obtained; and heating and calcining the precursor of the cobalt / nitrogen-doped carbon composite material in a protective atmosphere to obtain the cobalt nanoparticle / nitrogen-doped carbon composite material. According to the invention, plant cells are used as a template, and uniform loading of cobalt nanoparticles is realized through a self-absorption function of the plant cells, so that an efficient photocatalyst is developed, and efficient photocatalytic degradation of chloroquine phosphate and various organic pollutants in a water body is realized.

The invention provides plants of the basil cultivar designated ‘PAS1699933’. The invention thus relates to the plants, cells, plant parts, and tissue cultures of the cultivar ‘PAS1699933’, and to methods for producing a basil plant produced by crossing a basil plant of cultivar ‘PAS1699933’ with another basil plant, such as a plant of another cultivar. The invention further relates to basil seeds and plants produced by crossing plants of cultivar ‘PAS1699933’ with plants of another cultivar. The invention further relates to the genetic complements and hybrid genetic complements of plants of cultivar ‘PAS1699933’.

The invention relates to the technical field of plant extraction, in particular to a preparation method of a plant cell exosome, the plant cell exosome and application of the plant cell exosome. The preparation method comprises the following steps: after the surface of a plant leaf is scratched, inoculating the plant leaf into a first culture medium for induction culture, then transferring the plant leaf into a second culture medium for subculture to obtain a callus, and then adding the callus into a buffer solution for grinding to obtain a filtrate; centrifuging the filtrate again to obtain supernate; and adding an exosome complexing agent into the supernate, incubating overnight, centrifuging, and freeze-drying to obtain the exosome. The first culture medium comprises an MS culture medium and the following components added into the MS culture medium: pullulan, 2, 4-D, agar and NAA; the second culture medium comprises an MS culture medium and the following components added into the MS culture medium: pullulan, 2, 4-D, agar, NAA and sodium phytate. The preparation method can increase the protein content in the exosome.

The invention discloses tobacco bacterial wilt biocontrol burkholderia and application thereof, relates to the technical field of agricultural microorganisms, and is technically characterized in that the burkholderia S8288 is preserved in China General Microbiological Culture Collection Center (CGMCC) in October 2024, the preservation number of the burkholderia S8288 is CGMCC NO.32293, the preservation number of the burkholderia S8288 is CGMCC NO.32293, and the preservation number of the burkholderia S8288 is CGMCC NO.32293. The 16S rRNA (ribosomal Ribonucleic Acid) gene sequence of the Burkholderia sp. S8288 strain is as shown in SEQ ID No. 1. The strain has a good inhibition effect on pathogenic fine diseases and oomycetes such as Coreiella solani causing tobacco bacterial wilt and phytophthora nicotianae causing tobacco black shank, and the prevention effect on the tobacco bacterial wilt reaches 50%. The strain can induce death of plant cells and stimulate expression of plant-related immune genes. Besides, the Burkholderia sp. S8288 can decompose potassium, hydrolyze inorganic phosphorus and organic phosphorus and produce protease and NH3, and has potential capabilities of improving soil nutrition and promoting crop growth, and greenhouse tests show that the strain can significantly promote tobacco growth and development.

Aspects of the present disclosure relate to genetically modified plants and / or algae with increased carbon use efficiency as a result of an increased ability for bicarbonate to cross membranes within plant cells. Other aspects of the present disclosure relate to methods of making such plants and / or algae as well as cultivating these genetically modified plants to increase carbon use efficiency and / or growing these genetically modified algae to increase carbon use efficiency.

The invention discloses a method for inducing plant disease resistance by using a hydrolase active mutant of xyloglucan specific glucanase XEG1. A pichia pastoris eukaryotic expression system is utilized to obtain the purified hydrolase activity mutant XEG1E136D protein of the phytophthora sojae core pathogenic factor xyloglucan specific glucanase XEG1, and the hydrolase activity mutant XEG1E136D protein does not have the hydrolase activity of plant cell walls and can activate the systemic resistance of plants. The XEG1E136D is overexpressed into a biocontrol bacterium trichoderma harzianum by utilizing an agrobacterium-mediated fungal transformation optimization system, and the XEG1E136D is proved to be capable of secreting XEG1E136D protein to the outside of cells and activating the broad-spectrum systemic resistance of plants. The XEG1E136D protein expressed by the trichoderma harzianum can be used as a plant vaccine to improve the prevention and treatment effect, and the trichoderma harzianum XEG1E136D protein has a wide application prospect in the field of plant disease prevention and treatment.

The invention relates to construction and application of a plant cell expression tool, and particularly provides a nucleic acid construct which comprises a chimeric promoter and a coding sequence of a fusion protein, wherein the chimeric promoter comprises a protein binding region and a core promoter sequence, the fusion protein comprises a nucleic acid binding structural domain and a transcriptional activation structural domain, and the expression tool can improve expression of target protein in plant cells.

The invention relates to a preparation method of core-shell nanoparticles with photo-thermal-phase change dual functions and intracellular delivery application of the core-shell nanoparticles, and belongs to the field of nano materials and cell delivery. The invention provides a photothermal-phase change bifunctional core-shell nanoparticle applied to intracellular delivery of biomacromolecules, the nanoparticle is of a core-shell structure, a high-molecular polymer is taken as a shell, liquid fluorocarbon and a photothermal material are taken as a core, the nanoparticle is prepared by an ultrasonic emulsification method, and the preparation method is simple. The boiling point of the liquid fluorocarbon is low, and the photo-thermal material has good photo-thermal conversion performance, so that the core-shell nanoparticles can quickly generate photo-thermal steam microbubbles under laser induction. Under the synergistic effect of laser and core-shell nanoparticles, generated steam microbubbles are induced to generate micropores in plant cell walls and cell membranes or animal cell membranes, so that the physical barrier of the cell walls and the cell membranes is overcome, and the efficiency of delivering exogenous functional biomacromolecules into cells is remarkably improved.

The invention relates to the technical field of food, medicine and health care products, and discloses a composition containing rosemary exosome vesicles and application of the composition in anti-inflammatory, the composition is formed by mixing plant cell-derived exosome vesicles and functional active ingredients, the composition comprises the following components in percentage by mass: 45-55% of rosemary exosome and 45-55% of turmeric exosome; the preparation method of the composition containing the rosemary exosome vesicles comprises the following steps: S1, taking a certain amount of fresh rosemary and turmeric plants, removing root hairs and impurities, washing the fresh plants with domestic drinking water, washing the fresh plants with purified water for three times, and finally washing the fresh plants with ultrapure water for three times for 3-5 minutes each time; the product can carry various bioactive molecules, transfer cell signals in a transboundary manner, participate in anti-inflammation, inhibit tumor cell proliferation and promote normal tissue repair, has an anti-inflammatory effect, inhibits release of inflammatory factors, improves the immune function of a body and adjusts the immune balance state, and can play a role in neovascularization diseases of the body.

The invention discloses a plant cell activating factor as well as a preparation method and application thereof. The plant cell activating factor contains active ingredients such as resveratrol, quercetin, chlorogenic acid and the like. The active ingredients of the plant cell activating factor are obtained from natural plants, so that the plant cell activating factor has no pollution to the environment, is safe and harmless to human bodies and animals, and meets the requirements of green agricultural development. Experiments prove that the plant cell activating factor disclosed by the invention can effectively regulate the physiological activity of plant cells, activate the growth potential of plants, promote the development of plant root systems, thick and strong stalks and dark green leaves, and remarkably improve the growth speed and biomass accumulation of the plants, thereby increasing the yield of crops.

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ω3 destaurases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and / or stably transforming cells, particularly plant cells, with multiple genes.

A cotton variety FM 765AX is disclosed. Seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 765AX with other plants are also provided. Plants and varieties produced by the method of essential derivation from plants of FM 765AX and to plants of FM 765AX reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 765AX are provided.

The invention relates to an extraction method, a modification method and application of dendrobium nobile outer vesicles. The method comprises the following steps: placing dendrobium nobile leaves in a phosphate buffer solution, crushing and separating to ensure that plant cells are fully broken and outer vesicles are released; then a differential centrifugation method is combined with sucrose gradient centrifugation, finally, the outer vesicles in a sucrose solution with the mass concentration of 30-45% are selected, and the extracted dendrobium nobile outer vesicles are moderate in mass and high in purity. Then cholesterol structural analogues such as sterol compounds and triterpenoid saponin active molecules are embedded into a phospholipid bilayer of the dendrobium nobile outer vesicles to modify the dendrobium nobile outer vesicles, and the modification strategy can effectively improve the stability of the dendrobium nobile outer vesicles in a complex physiological environment; and the treatment effect can be enhanced through the synergism of active molecules and endogenous components of the vesicles.

The present invention relates to the observation of organic and inorganic processes by measuring radiation emitted by p-photon activity (e.g., p-photon radiation). In at least one embodiment, a method for measuring p-radiation is used to detect structural defects, safely synthesize toxic compounds, and track the growth of organisms inside a human body. Methods and systems are disclosed using electromagnetic radiation, called p-photons, with the help of sensors and artificial intelligence, to read spatial-temporal physical processes in inorganic material, plant cells, and living organs; broadcast information associated with those processes; and store and analyze that information.

The invention relates to tilletia foetida, in particular to a tilletia foetida effect protein TlRlpA and application thereof. The invention provides a Tilletia foetida effect protein, and the amino acid sequence of the Tilletia foetida effect protein is shown as SEQ ID NO: 2, SEQ ID NO: 4 or SEQ ID NO: 6. The effector protein can effectively inhibit programmed death of plant cells induced by BAX, and plays an important role in inhibiting a plant defense reaction process. The invention lays a foundation for deeply researching the pathogenesis of the tilletia foetida, excavating the tilletia foetida-resistant gene of the wheat and developing the tilletia foetida-resistant wheat variety.

The invention belongs to the field of gene engineering, and particularly relates to application of a willow uridine diphosphate glucose-4-epimerase gene SpUGE1 in plant cell wall component remodeling, organelle cadmium content reduction and cadmium resistance improvement. According to the invention, the SpUGE1 gene in willow is extracted, and is transferred into arabidopsis thaliana by constructing a recombinant plasmid, so that the biological activity, Cd < 2 + > concentration and the like of wild arabidopsis thaliana and transgenic arabidopsis thaliana are compared. The result of the embodiment shows that the transgenic arabidopsis thaliana has certain tolerance to cadmium stress, the cell wall of the transgenic arabidopsis thaliana is obviously thickened, and the content of cadmium in organelles is obviously reduced.