913 results about "Plant cell" patented technology

A resistance gene TaCAT to FCR of wheat and an application thereof are provided, which relates to the field of biological genetic engineering technologies. The nucleotide sequence of a genome where the TaCAT gene is located, the nucleotide sequence of a cDNA, the nucleotide sequence of a CDS and a coded protein sequence are respectively as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. An InDel marker is developed for identifying different allelotypes of the TaCAT gene. An application of the TaCAT gene for preparation of transgenic plant cells and plant breeding is provided. The molecular genetic basis of the resistance to FCR is disclosed, an important role is played in improving the resistance to FCR by utilizing a genetic engineering technology, and a new way is provided for breeding a new wheat variety with disease-resistance and safety.

The present invention discloses a multi-modal system for real-time plant hydration monitoring and irrigation management. The system comprises at least one acoustic sensor configured to emit controlled sound waves through plant tissues and detect corresponding vibrations and resonance frequencies using sensitive microphones or piezoelectric sensors to assess plant hydration. The system includes at least one bioelectrical sensor for monitoring bioelectrical signals. At least one nanotechnology-based sensor is configured to detect molecular changes in water content within plant cells through embedded or externally applied nanosensors. An aerial imaging device mounted on an unmanned aerial vehicle captures data related to canopy temperature, leaf color, and hydration indicators using infrared, multi-spectral, and thermal cameras. A central processing unit receives and analyzes data using predictive models, integrates the data via data fusion algorithm to generate a plant hydration profile, and controls water delivery through an irrigation management unit based on the hydration profile.

The invention discloses a preparation method of an anti-aging cosmetic containing exosome, and relates to the technical field of cosmetic preparation, the cosmetic is prepared from the following components in percentage by mass: 1.01%-5.05% of exosome wrapped by liposome, 0.5%-3% of an antioxidant, 1%-3% of a centella asiatica extract, 1%-3% of a rhodiola rosea extract, 10%-15% of a humectant and the balance of a basic matrix; wherein the exosome wrapped by the liposome is a mixture formed by mixing an exosome secreted by adipose-derived mesenchymal stem cells and an exosome secreted by plant cells according to a mass ratio of 1.5: 1.2; the plant is ginseng. According to the skin care product, the antioxidant, the centella asiatica extract and the exosome act together to improve the DPPH clearance rate of the skin care product, the skin brightness is improved, the roughness is reduced, and the excellent anti-aging performance is achieved. The lipid exosome-antioxidant-centella asiatica ternary system disclosed by the invention has a multi-target synergistic effect.

The invention relates to application of turmeric rhizome extract emulsifiable concentrate in preventing and treating corn leaf spot and tomato gray mold. Compared with the prior art, the turmeric rhizome extract missible oil disclosed by the invention contains an effective substance aromatic turmerone, and the aromatic turmerone can reduce the spore germination rate and sporulation quantity of pathogenic bacteria, increase the permeability of hypha cell membranes and cause overflow of contents, so that normal growth of hyphae is inhibited, and an antibacterial effect is achieved. Aromatic turmerone acts on plants, so that plant cells generate more polyphenol and flavonoid substances, and the immunity of the plants is improved. Pharmacodynamic test results in fields in two fields show that the 6.5% turmeric rhizome extract emulsifiable concentrate can be used for stably and effectively preventing and treating corn northern leaf blight and tomato gray mold after being diluted by 150-300 times, and also has a yield increasing effect.

A transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63717 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63717, and methods of using and detecting Gm_CSM63717 are also provided. Soybean plants containing the event Gm_CSM63717 exhibit tolerance to PPO inhibitors.

Provided herein, inter alia, is an engineered non-plant cell that produces a hyoscyamine alkaloid product, a precursor of a hyoscyamine alkaloid product, or a derivative of a hyoscyamine alkaloid product. Also described is a method for producing a scopolamine alkaloid, a precursor of a scopolamine alkaloid product, or a derivative of a scopolamine alkaloid product using a cell.

The invention discloses a targeted delivery method of a plant-derived biological regulator, and relates to the technical field of bioengineering, and the targeted delivery method comprises the following steps: in the application process of an electroporation technology, monitoring and acquiring cell membrane lipid change data information in real time through a biosensor technology; the method comprises the following steps: preprocessing original data acquired by a biosensor, and organizing and storing the preprocessed data according to rules to form a structured data set; the system can accurately control the state of the plant cell membrane by monitoring the lipid change of the cell membrane in real time and combining intelligent evaluation and dynamic adjustment of the electric field parameters. When membrane lipid is oversaturated, the electric field intensity and the pulse frequency are dynamically adjusted, membrane damage is reduced, cell integrity is protected, and meanwhile effective delivery of allogenic materials is ensured. According to the method, the stability and the accuracy of an electroporation technology are improved, the targeted delivery of the plant-derived biological regulator is optimized, and the application potential of the plant-derived biological regulator in the fields of agriculture and biomedicine is expanded.

The invention provides plants of the basil cultivar designated ‘PAS1699933’. The invention thus relates to the plants, cells, plant parts, and tissue cultures of the cultivar ‘PAS1699933’, and to methods for producing a basil plant produced by crossing a basil plant of cultivar ‘PAS1699933’ with another basil plant, such as a plant of another cultivar. The invention further relates to basil seeds and plants produced by crossing plants of cultivar ‘PAS1699933’ with plants of another cultivar. The invention further relates to the genetic complements and hybrid genetic complements of plants of cultivar ‘PAS1699933’.

The invention relates to the technical field of plant extraction, in particular to a preparation method of a plant cell exosome, the plant cell exosome and application of the plant cell exosome. The preparation method comprises the following steps: after the surface of a plant leaf is scratched, inoculating the plant leaf into a first culture medium for induction culture, then transferring the plant leaf into a second culture medium for subculture to obtain a callus, and then adding the callus into a buffer solution for grinding to obtain a filtrate; centrifuging the filtrate again to obtain supernate; and adding an exosome complexing agent into the supernate, incubating overnight, centrifuging, and freeze-drying to obtain the exosome. The first culture medium comprises an MS culture medium and the following components added into the MS culture medium: pullulan, 2, 4-D, agar and NAA; the second culture medium comprises an MS culture medium and the following components added into the MS culture medium: pullulan, 2, 4-D, agar, NAA and sodium phytate. The preparation method can increase the protein content in the exosome.

Aspects of the present disclosure relate to genetically modified plants and / or algae with increased carbon use efficiency as a result of an increased ability for bicarbonate to cross membranes within plant cells. Other aspects of the present disclosure relate to methods of making such plants and / or algae as well as cultivating these genetically modified plants to increase carbon use efficiency and / or growing these genetically modified algae to increase carbon use efficiency.

The invention discloses a method for inducing plant disease resistance by using a hydrolase active mutant of xyloglucan specific glucanase XEG1. A pichia pastoris eukaryotic expression system is utilized to obtain the purified hydrolase activity mutant XEG1E136D protein of the phytophthora sojae core pathogenic factor xyloglucan specific glucanase XEG1, and the hydrolase activity mutant XEG1E136D protein does not have the hydrolase activity of plant cell walls and can activate the systemic resistance of plants. The XEG1E136D is overexpressed into a biocontrol bacterium trichoderma harzianum by utilizing an agrobacterium-mediated fungal transformation optimization system, and the XEG1E136D is proved to be capable of secreting XEG1E136D protein to the outside of cells and activating the broad-spectrum systemic resistance of plants. The XEG1E136D protein expressed by the trichoderma harzianum can be used as a plant vaccine to improve the prevention and treatment effect, and the trichoderma harzianum XEG1E136D protein has a wide application prospect in the field of plant disease prevention and treatment.

The invention relates to a preparation method of core-shell nanoparticles with photo-thermal-phase change dual functions and intracellular delivery application of the core-shell nanoparticles, and belongs to the field of nano materials and cell delivery. The invention provides a photothermal-phase change bifunctional core-shell nanoparticle applied to intracellular delivery of biomacromolecules, the nanoparticle is of a core-shell structure, a high-molecular polymer is taken as a shell, liquid fluorocarbon and a photothermal material are taken as a core, the nanoparticle is prepared by an ultrasonic emulsification method, and the preparation method is simple. The boiling point of the liquid fluorocarbon is low, and the photo-thermal material has good photo-thermal conversion performance, so that the core-shell nanoparticles can quickly generate photo-thermal steam microbubbles under laser induction. Under the synergistic effect of laser and core-shell nanoparticles, generated steam microbubbles are induced to generate micropores in plant cell walls and cell membranes or animal cell membranes, so that the physical barrier of the cell walls and the cell membranes is overcome, and the efficiency of delivering exogenous functional biomacromolecules into cells is remarkably improved.

The invention discloses a plant cell activating factor as well as a preparation method and application thereof. The plant cell activating factor contains active ingredients such as resveratrol, quercetin, chlorogenic acid and the like. The active ingredients of the plant cell activating factor are obtained from natural plants, so that the plant cell activating factor has no pollution to the environment, is safe and harmless to human bodies and animals, and meets the requirements of green agricultural development. Experiments prove that the plant cell activating factor disclosed by the invention can effectively regulate the physiological activity of plant cells, activate the growth potential of plants, promote the development of plant root systems, thick and strong stalks and dark green leaves, and remarkably improve the growth speed and biomass accumulation of the plants, thereby increasing the yield of crops.

A cotton variety FM 765AX is disclosed. Seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 765AX with other plants are also provided. Plants and varieties produced by the method of essential derivation from plants of FM 765AX and to plants of FM 765AX reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 765AX are provided.

The invention relates to an extraction method, a modification method and application of dendrobium nobile outer vesicles. The method comprises the following steps: placing dendrobium nobile leaves in a phosphate buffer solution, crushing and separating to ensure that plant cells are fully broken and outer vesicles are released; then a differential centrifugation method is combined with sucrose gradient centrifugation, finally, the outer vesicles in a sucrose solution with the mass concentration of 30-45% are selected, and the extracted dendrobium nobile outer vesicles are moderate in mass and high in purity. Then cholesterol structural analogues such as sterol compounds and triterpenoid saponin active molecules are embedded into a phospholipid bilayer of the dendrobium nobile outer vesicles to modify the dendrobium nobile outer vesicles, and the modification strategy can effectively improve the stability of the dendrobium nobile outer vesicles in a complex physiological environment; and the treatment effect can be enhanced through the synergism of active molecules and endogenous components of the vesicles.

The present invention relates to the observation of organic and inorganic processes by measuring radiation emitted by p-photon activity (e.g., p-photon radiation). In at least one embodiment, a method for measuring p-radiation is used to detect structural defects, safely synthesize toxic compounds, and track the growth of organisms inside a human body. Methods and systems are disclosed using electromagnetic radiation, called p-photons, with the help of sensors and artificial intelligence, to read spatial-temporal physical processes in inorganic material, plant cells, and living organs; broadcast information associated with those processes; and store and analyze that information.

The invention relates to tilletia foetida, in particular to a tilletia foetida effect protein TlRlpA and application thereof. The invention provides a Tilletia foetida effect protein, and the amino acid sequence of the Tilletia foetida effect protein is shown as SEQ ID NO: 2, SEQ ID NO: 4 or SEQ ID NO: 6. The effector protein can effectively inhibit programmed death of plant cells induced by BAX, and plays an important role in inhibiting a plant defense reaction process. The invention lays a foundation for deeply researching the pathogenesis of the tilletia foetida, excavating the tilletia foetida-resistant gene of the wheat and developing the tilletia foetida-resistant wheat variety.

The invention relates to the technical field of plant cell engineering, and particularly discloses a plant protoplast semi-fusion breeding method which comprises the following three stages of seedling material selection, preparation, fusion, induction and cultivation. A large number of fused plant seedlings are obtained through a series of operations and used for large-scale planting. The method comprises the following steps: selecting excellent varieties of a plant A and a plant B, respectively preparing the plant A and the plant B into protoplasts in a preparation process, culturing the protoplasts into corresponding calluses, and performing half-cell fusion on a fusion part to obtain mosaic calluses. Then, the callus of the plant A to be used as the root is induced to locally induce adventitious roots, the callus of the plant B to locally induce adventitious buds, and finally, breeding is carried out to obtain the super seedlings. According to the method, the grafting principle is combined, the degree and conditions of cell fusion are optimized, the breeding cost is reduced, meanwhile, it is ensured that the fusion body can stably express the excellent characters of parents at different parts, and agricultural large-scale production is facilitated.

The invention discloses application of a scutellaria baicalensis SbMYB8 gene in promotion of anthocyanin synthesis, the application comprises the steps that the SbMYB8 gene is constructed to a plant expression vector and introduced into plant cells to promote synthesis of plant anthocyanin, and the nucleotide sequence of the SbMYB8 gene is shown as SEQ ID NO: 1, or the amino acid sequence coded by the SbMYB8 gene is shown as SEQ ID NO: 2. The MYB transcription factor SbMYB8 gene with the function of promoting anthocyanin synthesis is cloned and identified from the scutellaria baicalensis for the first time, and the blank of a transcription regulation mechanism for regulating anthocyanin metabolism in the scutellaria baicalensis is filled; the SbMYB8 gene can significantly promote accumulation of anthocyanin in various plants, shows good heterologous expression stability and adaptability, and has wide cross-species application potential.

A transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63717 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63717, and methods of using and detecting Gm_CSM63717 are also provided. Soybean plants containing the event Gm_CSM63717 exhibit tolerance to PPO inhibitors.

The invention discloses a plant cell vesicle efficient extraction method based on the synergistic effect of three-dimensional gradient micro vortex and sound wave fluidization and application of the plant cell vesicle efficient extraction method. According to the plant cell vesicle extraction method provided by the invention, high-yield, high-purity and high-activity extraction of plant cell vesicles is realized through an active enzymolysis-acoustic cracking-three-dimensional gradient separation full-chain process, and the plant cell vesicle extraction method can be used for large-scale production. The method overcomes the defects of low yield and purity, chemical pollution, low extraction efficiency, uncontrollable vesicle size, easily damaged structure, long time and the like of the traditional extraction method, and can be used for producing and preparing more cosmetics and medicines containing plant cell vesicles on a large scale. The method systematically solves the global problem of extraction and application of the plant vesicles through multidisciplinary cross innovation (acoustics, fluid mechanics and molecular bionics), is high in extraction efficiency, good in activity effect and comprehensive and advanced in technical parameters, and has important significance on extraction and development of the plant cell vesicles.

Methods and materials for modulating biomass levels in plants are disclosed. For example, nucleic acids encoding biomass-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased biomass levels and plant products produced from plants having increased biomass levels.

The invention provides to improved methods for the modification of genes in plant cells, and plants and seeds derived therefrom. More specifically, the invention relates to the increased efficiency of targeted gene mutation by combining gene repair oligonucleotides with approaches that enhance the availability of components of the target cell gene repair mechanisms.

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

Chimeric GRF-GIF polypeptides that can be used to improve the capacity of plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants are disclosed. Also disclosed are plant cells comprising the chimeric polypeptides and related methods for improving the capacity of the plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased soybean cyst nematode (SCN) resistance are provided. Also provided are methods of breeding plants having alleles associated with increased SCN resistance and methods of identifying and selecting plants having alleles associated with increased SCN resistance. Transgenes and genome-edited plants comprising gene alleles associated with increased SCN resistance are provided.

Methods and materials for modulating biomass levels in plants are disclosed. For example, nucleic acids encoding biomass-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased biomass levels and plant products produced from plants having increased biomass levels.

The present disclosure provides fatty acid desaturase 2 (FAD2) genes and plants and / or plant cells bearing one or more mutations in two or more FAD2 genes; as well as methods of making and using such plants. In some embodiments, plants producing seed oil with high oleic acid content are provided.

A cotton variety FM 823AXTP is disclosed. Seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 823AXTP with other plants are also provided. Plants and varieties produced by the method of essential derivation from plants of FM 823AXTP and to plants of FM 823AXTP reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 823AXTP are provided.

The invention discloses a radix gentianae callus cell culture and application method, and relates to the technical field of plant biology.The method comprises the following steps that in the laser irradiation process, a real-time spectrum analyzer is used for monitoring radix gentianae tissue, and absorption characteristic information of the plant tissue to light with different wavelengths is obtained. By combining the real-time spectral analysis and the machine learning model, the scheme can accurately monitor the interaction between the laser wavelength and the photosensitive component in the plant tissue, and by intelligently evaluating and dynamically adjusting the laser wavelength, the excessive absorption of laser energy is avoided, and the photochemical reaction and cell damage are reduced. The method not only reduces the potential damage of laser irradiation to plant cells, but also optimizes the synthesis process of medicinal components, and ensures the high efficiency, repeatability and reliability of callus culture. According to the scheme, powerful support is provided for improving the accuracy of experimental results and commercialized application of the gentiana scabra bunge.