356 results about "Plant cell" patented technology

The invention relates to tilletia foetida, in particular to a tilletia foetida effect protein TlRlpA and application thereof. The invention provides a Tilletia foetida effect protein, and the amino acid sequence of the Tilletia foetida effect protein is shown as SEQ ID NO: 2, SEQ ID NO: 4 or SEQ ID NO: 6. The effector protein can effectively inhibit programmed death of plant cells induced by BAX, and plays an important role in inhibiting a plant defense reaction process. The invention lays a foundation for deeply researching the pathogenesis of the tilletia foetida, excavating the tilletia foetida-resistant gene of the wheat and developing the tilletia foetida-resistant wheat variety.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased soybean cyst nematode (SCN) resistance are provided. Also provided are methods of breeding plants having alleles associated with increased SCN resistance and methods of identifying and selecting plants having alleles associated with increased SCN resistance. Transgenes and genome-edited plants comprising gene alleles associated with increased SCN resistance are provided.

The present disclosure provides fatty acid desaturase 2 (FAD2) genes and plants and / or plant cells bearing one or more mutations in two or more FAD2 genes; as well as methods of making and using such plants. In some embodiments, plants producing seed oil with high oleic acid content are provided.

The invention discloses a preparation method of a plant source nano vesicle and the plant source nano vesicle. According to the preparation method disclosed by the invention, through extensive and deep research and a large amount of screening optimization, the coix seed-derived nano vesicles (SCDNVs) and the gastrodia elata-derived nano vesicles (RGDNVs) which are high in concentration and high in quality are successfully extracted by using the preparation method disclosed by the invention; meanwhile, the invention further researches the anti-tumor effect and the anti-oxidation effect of the prepared plant source nano vesicle. Through screening of conditions of the preparation method, an ultrasonic combined centrifugal method (further comprising screening of ultrasonic conditions, such as ultrasonic power / ultrasonic time and the like) adopted in the invention can overcome the defects that the membrane structure of the vesicle is damaged and the form of the vesicle is not complete enough in the traditional method; therefore, the plant extracellular vesicles which are small in loss, high in concentration, good in stability, more uniform in vesicle particle size, small in particle size span, high in yield and high in quality are obtained.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The present invention relates to a recombinant construct comprising: a first expression cassette comprising a fluorescence enhancing factor; wherein the amino acid sequence of the fluorescence enhancement factor is as shown in SEQ ID NO: 2, or an amino acid sequence which at least has 60%, 65%, 70%, 75%, 80%, 85% or 90% identity with the SEQ ID NO: 2 and is functionally equivalent to the protein as shown in the SEQ ID NO: 2; the first expression cassette is configured to increase the self-luminous intensity of a plant, plant tissue, or cell. According to the application, the LumEnhancer gene is further introduced on the basis of an FBP biological self-luminous system, so that the physiological activity of transgenic plant cells is effectively improved, and the energy metabolism level of the plant is further enhanced, thereby providing a more sufficient energy basis for a bioluminescence reaction and effectively enhancing the luminous intensity of the whole plant.

The invention discloses a plant cell extract-containing traditional Chinese medicine aromatic penetration enhancer and a preparation method thereof, and the penetration enhancer comprises the following components in parts by mass: 8-15 parts of storax nano-vesicles, 3-8 parts of agilawood nano-vesicles, 1-5 parts of refined turpentine, 0.5-3 parts of panax notoginseng saponins, 0.5-3 parts of rhododendron dauricum oil and 20-35 parts of grape seed oil, the storax nano-vesicles and the agilawood nano-vesicles are exosome-like nano-vesicles extracted from plant secretion, and the particle size of the vesicles is 50-200 nm. Skin interstices are opened, skin meridians and blood are dredged, and accordingly relieving, inflammation resisting, redness improving and skin barrier repairing are achieved.

The invention relates to the technical field of biological medicines, in particular to a preparation method of protein cross-linked nano affinity microspheres, which comprises the following steps: preparing carboxylated silicon dioxide nano microspheres with the particle size of 500nm by adopting a sol-gel method, mixing tetraethoxysilane and ammonia water according to the volume ratio of 1: (3 + / -1), reacting in an ethanol solution at 40 DEG C for 6 hours, filtering, washing, and drying to obtain the protein cross-linked nano affinity microspheres. And centrifuging to obtain a microsphere precipitate, washing with deionized water for three times to finally obtain a nano microsphere suspension of which the surface is rich in carboxyl, and controlling the solid content to be 10 + / -0.5 mg / mL. The capturing efficiency of the finally prepared protein cross-linked nano affinity microspheres for exosomes reaches 95.0%, the binding capacity reaches 28.5 mu g / mg, the purity of the extracted exosomes is higher than that of an ultracentrifugation method, the biological activity and structural integrity of animal cell exosomes and plant cell exosomes are completely kept, and the protein cross-linked nano affinity microspheres are suitable for large-scale industrial production. The problems that in a traditional exosome extraction method, the recovery rate is low, impurity pollution is serious, exosome activity is damaged, and the application range is limited are solved.

The invention relates to the technical field of traditional Chinese medicine extraction and concentration, and discloses an efficient and energy-saving traditional Chinese medicine micro powder extraction and concentration system. Firstly, plant cell walls are fully destroyed through a traditional Chinese medicine micro-powder wall breaking technology, and complete and quantitative conveying of micro-powder materials is ensured in combination with an air pressure balance residue removing system which is arranged in a storage tank and composed of an air disc, an air blowing pipe and an air blowing plug. And secondly, direct contact type hot air heating and stirring are adopted in the extraction tank, and a two-stage dust suppression structure of spraying and wet dust removal is arranged, so that efficient extraction and dust control are realized. And finally, the system recycles the residual components of the decoction dregs through a secondary extraction process, and adopts a membrane filtration and membrane concentration technology to replace the traditional heating concentration, so that the filtration and concentration of the decoction are completed at normal temperature. The method effectively solves the problems of low extraction rate, high energy consumption, easy damage of thermosensitive components and dust pollution in the traditional process, and realizes high efficiency, energy conservation and high quality of production of the traditional Chinese medicine extraction preparation.

The invention discloses an application of an overexpressed CrOASTL1 gene in improving the soluble protein content of leguminous forage, and belongs to the technical field of biology. 1, and the CDS sequence of the CrOASTL1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: cloning a CrOASTL1 gene segment, constructing a plant overexpression vector, carrying out plant transgenosis operation, and introducing into leguminous forage plant cells to obtain a leguminous forage transgenosis line with the overexpressed CrOASTL1 gene. The CrOASTL1 gene is over-expressed in medicago sativa, and the soluble protein content of leaves of the medicago sativa is remarkably increased by about 19%. The CrOASTL1 gene of chlamydomonas reinhardtii is applied to quality improvement of leguminous forage for the first time, the technical obstacle that the genetic relationship of species is relatively far is overcome, a brand new gene resource and an efficient technical approach are provided for cultivating a new variety of leguminous forage with high protein and high nutritional value, and the method has important significance in promoting sustainable development of grass and animal husbandry.

The invention belongs to the technical field of plant cytology, and particularly relates to a method for improving the transformation efficiency of plant protoplast, which comprises the following steps: preparing enzymatic hydrolysate: each liter of enzymatic hydrolysate contains 20 mmol of KCl, 5-20 g of cellulase, 2-6 g of macerozyme, 10 mmol of CaCl2, 1 g of BSA and the balance of water; performing leaf enzymolysis; obtaining a protoplast; preparing a competent protoplast; resuspending the MMG solution; and transforming and incubating the protoplast resuspension by using the target plasmid. According to the method, the high-quality protoplast can be obtained, and when the protoplast is applied to downstream experiments, the conversion efficiency and stability of the experiments can be remarkably improved.

The invention relates to the technical field of biology, in particular to a method for regulating and controlling plant sodium ion homeostasis and salt tolerance by a light-operated pump, which comprises the following steps: constructing systems of rhodopsin light-sensitive pumps KR2-T1.0, KR2-T2.0, KR2-T3.0 and KR2-T4.0 with different cell membrane localization and membrane loading efficiencies through gene optimization, connecting the systems with a retinaldehyde production gene Ret, then inserting into a plant expression vector pCAMBIA3300, and carrying out photosynthesis to obtain the plant sodium ion homeostasis and salt tolerance control plant sodium ion homeostasis and salt tolerance control plant sodium ion homeostasis and salt tolerance control plant sodium ion homeostasis and salt tolerance control plant sodium ion homeostasis and salt tolerance control plant sodium ion homeostasis. Transient expression of nicotiana benthamiana and stable expression of rape are achieved through agrobacterium tumefaciens mediation, the pump is activated through irradiation of green light of 530 nm + / -5 nm, active excretion in plant cells is driven, and accurate, dynamic and reversible regulation and control of the sodium ion steady state are achieved. According to the method, the technical bottlenecks that KR2 is poor in plant cell membrane localization and low in membrane loading efficiency are broken through, gradient regulation and control of efflux efficiency are achieved, the method is high in response speed, light energy serves as power, cell ATP is not depended on, exogenous chemical intervention is avoided, and the plant salt tolerance can be remarkably improved.

The present disclosure is directed to a split ribozyme biosensor system. Additionally, a genetically modified plant, plant tissue, or plant cell comprising the split ribozyme biosensor system is described. Methods for examining in vivo RNA expression in plants, plant cells or plant tissues are disclosed. Lastly, disclosed herein is a kit comprising the split ribozyme biosensor system.

The present invention relates generally to a plant cell-surface immune receptor engineered to modulate an immune response in a plant, cell or seed, as well as nucleic acid molecules encoding the engineered receptor, and methods of use. In particular, the present invention relates to a plant cell-surface immune receptor into which a coiled-coil (CC) motif is integrated to enhance or modify the plant's immune response, with utility in reducing pathogen infection and improving pathogen resistance.

The invention relates to the technical field of plant genetic engineering and agricultural planting, in particular to application and method of an SlGRXC9 gene in improvement of tomato cadmium stress tolerance, the number of the SlGRXC9 gene is Solyc07g053550, the core coding region sequence of the SlGRXC9 gene is SEQ ID NO.1, and the coded protein sequence is SEQ ID NO.2; through construction of SlGRXC9 overexpression transgenic tomatoes, heterologous expression of protein encoded by the gene in plant cells or use of the gene as a molecular breeding target, the system activity of antioxidant enzymes such as SOD, POD, CAT and GR in tomatoes can be enhanced, cadmium stress induced H2O2 and O2-accumulation and membrane lipid peroxidation damage are reduced, and absorption and accumulation of the tomatoes to cadmium ions are not affected; according to the method, the growth ability and physiological stability of the tomatoes under cadmium stress are remarkably improved, the risk of nutrient imbalance is avoided, the hereditary stability is high, and the method can be widely applied to safe production of the tomatoes in cadmium-contaminated soil and creation of stress-tolerant germplasm.

The invention discloses application of a rice cell wall CytoUGE gene in preparation of self-assembled large-size cellulose nanocrystals, and belongs to the field of plant genetic engineering and nano materials. The nucleotide sequence of the rice cell wall CytoUGE gene is as shown in SEQ ID NO. 3. Research finds that the rice cell wall CytoUGE gene can regulate and control plant cell wall polysaccharide metabolism key enzyme through heredity, direct regulation and control of the size and the crystallinity of the cellulose nanocrystal are achieved, and the cellulose nanocrystal obtained through rice stems over-expressing the rice cell wall CytoUGE gene is large in size and high in crystallinity; and a better reinforcing effect is achieved in the nano composite material. The invention provides a new technical approach and gene resources for high-valued utilization of plant biomass, and has dual application values and wide application prospects in variety breeding and functional material development.

The invention discloses a method for improving salt resistance of plants. According to the method, an LSU3 gene coding sequence shown in SEQ ID NO: 1 is introduced into a plant cell and an LSU3 protein is expressed, so that the viability and the growth condition of the plant in a salt stress environment are remarkably enhanced. The invention further provides a recombinant expression vector containing the gene, a construction method of the recombinant expression vector and a specific application scheme of the recombinant expression vector in improvement of the salt resistance of arabidopsis thaliana, and effective gene resources and technical means are provided for salt-resistant breeding of crops.

The invention provides an extraction method of high-content alpha-linolenic acid, and relates to the technical field of biological extraction. The extraction method of the high-content alpha-linolenic acid comprises the following steps: pretreating raw materials, destroying a plant cell wall structure through related operations of crushing and degreasing, releasing grease components, improving the subsequent extraction efficiency, removing phospholipid and free fat, and avoiding impurities influencing the purification effect; the method comprises the following steps: carrying out directional hydrolysis on lipase, cutting off sn-1 and 3 site ester bonds by utilizing lipase specificity, converting triglyceride into free fatty acid, reserving the structural integrity of sn-2 site alpha-linolenic acid, and enabling free alpha-linolenic acid to be more easily separated through subsequent distillation / chromatography compared with glyceride form. Through a silver ion complexing chromatography technology, alpha-linolenic acid and other unsaturated fatty acids with similar structures can be accurately separated, the purity of the final product stably reaches 98% or above, the pharmaceutical grade application standard is met, and the technical bottleneck that a traditional purification method is difficult to break through the purity of 90% is solved.

The present application relates to the technical field of molecular biology experiment, especially to a kind of plant organelle RNA end analysis method and its application.The present application provides a kind of plant organelle RNA end analysis method, comprising the following steps: RNA is treated with 5' polyphosphatase, in vitro cyclization, then double-end sequencing is carried out, filtering, to obtain clean read length;MeCi algorithm is used to identify clean read length;According to the identification result and the number of clean read length, the 5' and 3' end position of RNA, the end type, the end position of mRNA and ncRNA and the 5' end type of mRNA and ncRNA are judged.The method not only analyzes organelle RNA end modification and organelle RNA poly(A) length and source, but also can be used for screening the substrate RNA of RNase and RBP involved in plant organelle RNA end processing and stability.

Methods and materials for modulating biomass composition in plants are disclosed. For example, nucleic acids encoding biomass composition-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having altered biomass composition and plant products produced from plants having altered biomass composition.

The present disclosure belongs to the field of plant genetic engineering, and specifically relates to the use of a small molecule compound for improving the efficiency of callus induction, transformation and regeneration in plants. Provided in the present disclosure is a method for callus induction in plants or for improving the efficiency of callus induction in plants. The method comprises the step of culturing plant cells in the presence of LFM-A13 and / or HDAC8-IN-1. It is found in the present disclosure that the efficiency of callus induction, transformation and regeneration in plants can be improved by culturing the plant cells in the presence of the small molecule compound(s) LFM-A13 and / or HDAC8-IN-1.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing embryogenesis factor genes and / or morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing embryogenesis factor gene products and / or morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Embryogenesis factor proteins and / or developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

According to the present invention, a technique which enables the production of an antimicrobial peptide Persulcatusin at reduced cost has been developed. A plant cell into which a polynucleotide sequence that encodes a gene for a protease to which a signal peptide localized in an intercellular organelle is added and a polynucleotide sequence that encodes a Persulcatusin fusion protein to which a signal peptide localized in an intracellular organelle different from the aforementioned intracellular organelle is added are introduced is produced, wherein, in the Persulcatusin fusion protein, a sequence that is cleavable with the protease is disposed between Persulcatusin and a protein that fuses to the Persulcatusin. Thus, Persulcatusin, which can serve as a therapeutic agent for bovine mastitis, can be produced at low cost.

Provided herein are methods for selecting maize plants comprising a QTL associated with resistance to southern rust. Also provided are methods of producing a plant or plant cell comprising a QTL associated with resistance to southern rust. Also provided are resistant plants or plant cells themselves, as well as resistant plant seeds.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a spartina anglica ascorbic acid peroxidase SanAPX11 gene and application, the nucleotide sequence of the SanAPX11 gene is shown as SEQ ID NO.1, and the amino acid sequence of protein coded by the SanAPX11 gene is shown as SEQ ID NO.2. After the SanAPX11 gene is transferred into a cell in the form of a plant expression vector, the SanAPX11 gene is expressed in a plant cell, so that the stress resistance of the plant, particularly drought stress resistance, oxidative stress resistance, salt stress resistance and the like, can be improved.

The invention discloses a method for extracting a natural antioxidant from a cyperus esculentus shell fermentation product by using subcritical water, and belongs to the technical field of plant antioxidant extraction. The preparation method comprises the following steps: fermenting cyperus esculentus shell powder to obtain fermentation liquor; performing subcritical water extraction on the fermentation liquor to obtain an extracting solution; and filtering, purifying and freeze-drying the extracting solution to obtain the natural antioxidant. The plant cell walls can be selectively and mildly degraded and deconstructed by utilizing rich enzyme systems secreted by specific microorganisms in the growth and metabolism process. The biological pretreatment not only can destroy the physical barrier of the cell wall, but also can release active substances in a combined state in the cell wall, so that the active substances are converted into a free state, and the dissolution efficiency and the yield of subsequent extraction are greatly improved. Subcritical water extraction has the outstanding advantages of high extraction efficiency, high speed, no need or great reduction of the use amount of organic solvents, environmental friendliness and the like.

The present application relates to the technical field of biological cell culture, and particularly relates to a fixed bed device and method for improving secretion and expression of exosomes. The device comprises a fixed bed reactor, an ultrasonic generator, a connecting flange and a control unit. The ultrasonic generator is installed on the top of the fixed bed reactor through the connecting flange. The control unit is configured to control the ultrasonic generator to apply ultrasonic stimulation to the cells intermittently in the whole cycle of cell culture. The ultrasonic intensity is dynamically regulated in three stages according to the culture cycle. The present application uses pure physical stimulation without adding chemical reagents. Under the premise of maintaining normal growth and activity of cells, the yield of exosomes can be increased by more than 169%. The present application is suitable for animal cells and plant cells and has a wide industrial application prospect. The technical problems of low yield of exosomes, stimulation mode that may affect cell activity or introduction of chemical reagents that may interfere in the prior art can be effectively solved.

The present disclosure provides methods for inducing callus formation and plant regeneration from isolated protoplasts of grape. This technology allows for the production of non-chimeric gene edits in grape plants by allowing the delivery of DNA through the plant cell membrane with the recovery of whole plants from a single edited cell.

The invention discloses a preparation method of a plant cell loaded selenium disulfide compound, and belongs to the technical field of nano materials and daily chemicals. The preparation method comprises the following steps: S1, preparing a plant cell suspension; s2, wall breaking treatment of plant cells; s3, preparing a selenium disulfide nano dispersion liquid; and S4, loading of selenium disulfide. The method is simple in overall preparation and easy to popularize and apply, the defects of selenium disulfide can be well overcome, meanwhile, selenium disulfide is organically combined with plants, plant cells are used as carriers of the active components, unstable active components (such as selenium disulfide) are loaded into the plant cells, and the selenium disulfide is prepared. The invention has the advantages of improving stability, reducing irritation, realizing function synergy and improving the sense of the product. The plant cell loaded selenium disulfide compound prepared by the method disclosed by the invention can be effectively applied to various personal care products, and has extremely strong market competitiveness.

The application discloses a pear anthracnose resistance gene MYC2- like and application, and belongs to the field of plant genetic engineering. MYC2‑like The nucleotide sequence of the gene is shown as SEQ ID NO:1, the protein sequence encoded by the gene is shown as SEQ ID NO:2, the gene is significantly up-regulated in response to MeJA and anthracnose fungus induction; the gene is located in the plant cell nucleus and has a conserved bHLH domain; a MYC2‑like MYC2‑like The constructed overexpression vector is used for transforming Solanum lycopersicum callus, so that the resistance of the callus to Colletotrichum gloeosporioides is increased, and the application provides an important gene resource for cultivating pear anthracnose resistance varieties.