598 results about "Plant cell" patented technology

The invention relates to tilletia foetida, in particular to a tilletia foetida effect protein TlRlpA and application thereof. The invention provides a Tilletia foetida effect protein, and the amino acid sequence of the Tilletia foetida effect protein is shown as SEQ ID NO: 2, SEQ ID NO: 4 or SEQ ID NO: 6. The effector protein can effectively inhibit programmed death of plant cells induced by BAX, and plays an important role in inhibiting a plant defense reaction process. The invention lays a foundation for deeply researching the pathogenesis of the tilletia foetida, excavating the tilletia foetida-resistant gene of the wheat and developing the tilletia foetida-resistant wheat variety.

The invention discloses application of a scutellaria baicalensis SbMYB8 gene in promotion of anthocyanin synthesis, the application comprises the steps that the SbMYB8 gene is constructed to a plant expression vector and introduced into plant cells to promote synthesis of plant anthocyanin, and the nucleotide sequence of the SbMYB8 gene is shown as SEQ ID NO: 1, or the amino acid sequence coded by the SbMYB8 gene is shown as SEQ ID NO: 2. The MYB transcription factor SbMYB8 gene with the function of promoting anthocyanin synthesis is cloned and identified from the scutellaria baicalensis for the first time, and the blank of a transcription regulation mechanism for regulating anthocyanin metabolism in the scutellaria baicalensis is filled; the SbMYB8 gene can significantly promote accumulation of anthocyanin in various plants, shows good heterologous expression stability and adaptability, and has wide cross-species application potential.

The invention discloses a plant cell vesicle efficient extraction method based on the synergistic effect of three-dimensional gradient micro vortex and sound wave fluidization and application of the plant cell vesicle efficient extraction method. According to the plant cell vesicle extraction method provided by the invention, high-yield, high-purity and high-activity extraction of plant cell vesicles is realized through an active enzymolysis-acoustic cracking-three-dimensional gradient separation full-chain process, and the plant cell vesicle extraction method can be used for large-scale production. The method overcomes the defects of low yield and purity, chemical pollution, low extraction efficiency, uncontrollable vesicle size, easily damaged structure, long time and the like of the traditional extraction method, and can be used for producing and preparing more cosmetics and medicines containing plant cell vesicles on a large scale. The method systematically solves the global problem of extraction and application of the plant vesicles through multidisciplinary cross innovation (acoustics, fluid mechanics and molecular bionics), is high in extraction efficiency, good in activity effect and comprehensive and advanced in technical parameters, and has important significance on extraction and development of the plant cell vesicles.

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased soybean cyst nematode (SCN) resistance are provided. Also provided are methods of breeding plants having alleles associated with increased SCN resistance and methods of identifying and selecting plants having alleles associated with increased SCN resistance. Transgenes and genome-edited plants comprising gene alleles associated with increased SCN resistance are provided.

The present disclosure provides fatty acid desaturase 2 (FAD2) genes and plants and / or plant cells bearing one or more mutations in two or more FAD2 genes; as well as methods of making and using such plants. In some embodiments, plants producing seed oil with high oleic acid content are provided.

Methods and compositions for identifying, selecting and / or producing a pathogen resistant plant, plant cell or seed (e. g,, a maize plant, plant cell or seed) are provided. Polynucleotides and polypeptides are provided, which when expressed in a plant increase disease and / or pathogen resistance (e.g., to Fusarium stalk rot) of the plant. Plants, plant cells and seed are provided comprising such polypeptides and polynucleotides, as are methods for their use.

The invention is applicable to the technical field of biology, and provides a method for rapidly extracting plant rhizome extracellular vesicles from monosaccharide and compound enzyme. According to the method, the plant extracellular vesicles are extracted by an enzymolysis-monosaccharide solution extraction coupling method for the first time, and the defect that an extraction method is single is overcome. According to the method, the conditions are mild, the primary property, the integrity and the biological activity of the vesicle structure are kept, the extraction purity is high, and the particle size distribution is uniform and concentrated in the range of 20-300 nm. The total protein extraction amounts of glucose, galactose and fructose solutions are respectively increased by 36.09% + / -1.18%, 37.65% + / -2.83% and 36.83% + / -2.59% compared with those of PBS (Phosphate Buffer Solution) extraction treatment. In addition, the'single ultracentrifugation 'purification process overcomes the problem of time consumption of traditional gradient centrifugation, and the time is shortened by more than 3h compared with that of a traditional extraction method. The method breaks through the technical bottleneck of large-scale production of the plant extracellular vesicles, and has great application and popularization values.

Described are peptides including a membrane translocation domain having one or more cell penetrating peptide motifs, and a cargo moiety linked to the membrane translocation domain, wherein the cargo moiety includes a plant bioactive moiety. The at least one cell penetrating peptide motif is from 3 to 10 amino acid residues in length and has at least three arginine and / or lysine residues; or the at least one cell penetrating peptide motif is from 3 to 10 amino acid residues in length and has at least two arginine and / or lysine residues and at least one other cell penetrating peptide motif is from 2 to 8 amino acid residues in length and has at least two hydrophobic residues. Also described are methods of delivering a cargo moiety into a plant cell comprising contacting the plant cell with the peptide as disclosed herein.

The present invention belongs to the technical field of genetic engineering. Provided are a single-base editor, a deaminase used therein, and the use thereof. The technical problems to be solved are to identify a naturally occurring cytosine deaminase without sequence preference, construct a base editor, and improve the efficiency and scope of base editing. In order to solve the technical problems above, a cytosine base editor is provided. The cytosine base editor is a fusion protein, wherein the fusion protein is a protein containing a cytidine deaminase, a Cas protein and a uracil-DNA glycosylase inhibitor, and the cytidine deaminase is a protein having an amino acid sequence of positions 28-164 of SEQ ID NO. 2. Further provided is the use of the fusion protein above and a biomaterial related thereto in plant single-base editing. The single-base editor can improve the efficiency of cytosine base editing and accurately mediate the base mutation of a target, and is widely applicable in the cells of maize and even other plants.

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

The invention discloses a preparation method of a plant source nano vesicle and the plant source nano vesicle. According to the preparation method disclosed by the invention, through extensive and deep research and a large amount of screening optimization, the coix seed-derived nano vesicles (SCDNVs) and the gastrodia elata-derived nano vesicles (RGDNVs) which are high in concentration and high in quality are successfully extracted by using the preparation method disclosed by the invention; meanwhile, the invention further researches the anti-tumor effect and the anti-oxidation effect of the prepared plant source nano vesicle. Through screening of conditions of the preparation method, an ultrasonic combined centrifugal method (further comprising screening of ultrasonic conditions, such as ultrasonic power / ultrasonic time and the like) adopted in the invention can overcome the defects that the membrane structure of the vesicle is damaged and the form of the vesicle is not complete enough in the traditional method; therefore, the plant extracellular vesicles which are small in loss, high in concentration, good in stability, more uniform in vesicle particle size, small in particle size span, high in yield and high in quality are obtained.

The invention provides a honeysuckle MYB gene family transcription factor 13476. A honeysuckle HQT2 gene, an HQT2 gene promoter and a transcription factor 13476 are cloned respectively, and a yeast one-hybrid experiment proves that 13476 can directly interact with the HQT2 promoter. Furthermore, the regulation of the 13476 protein on the transcriptional activity of the HQT2 gene is analyzed by using a dual luciferase system in tobacco, and the 13476 is proved to have an inhibiting effect on the promoter of the HQT2. Tobacco transient transformation results show that the content of chlorogenic acid in plant cells can be increased by 30-40% through heterologous transient expression of the LjHQT2 gene, and the content of chlorogenic acid reaches the level of 1.59 mg / g. The RNAi method is adopted, molecules aiming at Lj13476 are transiently expressed in honeysuckle, the content of chlorogenic acid can be increased by 10%-20%, the content of isochlorogenic acid A can be increased by 5%-30%, and the content of isochlorogenic acid C can be increased by 7%-20%. The interaction mechanism of the honeysuckle HQT2 gene and the transcription factor 13476 is studied, a certain basis is provided for the regulation mechanism of chlorogenic acid, and the HQT2 gene and the transcription factor 13476 are of great significance for breeding high-quality honeysuckle in the future.

Disclosed herein are methods for rapid transformation, genome editing, and regeneration of dicot plants. The methods disclosed herein involve transforming plant cells and explants with recombinant DNA to achieve genome edits. The methods enhance transformation and regeneration efficiency and reduce the time to produce transgenic plants.

The invention discloses application of a brassica juncea gene BjuABCG42-A03 in regulating and controlling pollen germination and seed setting amount of brassica juncea, and relates to the technical field of plant genetic engineering, the amino acid sequence of the protein BjuABCG42-A03 is as shown in SEQ ID No.1, and meanwhile, the invention provides a method for cultivating a transgenic plant capable of improving the pollen germination rate or seed setting amount. The method comprises the following steps: constructing a recombinant plant expression vector containing a gene for coding protein BjuABCG42-A03; the constructed recombinant plant expression vector is transformed into agrobacterium and then transformed into a plant or a plant cell, and the gene of the coded protein BjuABCG42-A03 is over-expressed in the plant, so that the pollen germination rate or the seed setting amount of the plant is increased. Based on gene information of arabidopsis ABCG42 (At4g15233), a BjuABCG42-A03 gene is homologous cloned in a mustard genome, in-vivo and in-vitro germination experiments show that the BjuABCG42-A03 gene affects the elongation length and seed bearing quantity of a pollen tube, and a research direction is provided for solving the problem of low pollen fertility or low seed setting quantity of mustard subsequently.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention relates to the technical field of polysaccharide extraction, and discloses a method for extracting high-purity pectin through cooperation of pulse microwave and low temperature, which comprises the following steps: applying a microsecond-level microwave pulse sequence corresponding to inherent acoustic resonant frequency of raw material particles, and monitoring net absorption power and acoustic signals on line, so as to adaptively control frequency locking and pulse form in a closed-loop manner; according to the invention, the electromagnetic energy of microwaves is converted into instantaneous mechanical work, and efficient structural damage to the plant cell walls from inside to outside is realized through a resonant coupling effect on the premise of maintaining the low temperature of the system; the online self-adaptive closed-loop control mechanism ensures the whole-process optimization of the energy coupling efficiency and the process stability, so that the inherent contradiction that the product quality and the extraction efficiency are difficult to consider at the same time in the traditional extraction technology is solved.

This invention relates to the production of plant-made melanin nanoparticles. This comprises utilizing the agrobacterium infiltration of a plant or plant cell to initiate a plant cell response by way of tyrosinase enzymatic or similar biochemical reactions to form biosynthetic plant-made melanin nanoparticles. The plant-made melanin nanoparticles can be utilized for biomedical, healthcare, therapeutic, environmental, or industrial use among many other uses.

The present invention relates to a recombinant construct comprising: a first expression cassette comprising a fluorescence enhancing factor; wherein the amino acid sequence of the fluorescence enhancement factor is as shown in SEQ ID NO: 2, or an amino acid sequence which at least has 60%, 65%, 70%, 75%, 80%, 85% or 90% identity with the SEQ ID NO: 2 and is functionally equivalent to the protein as shown in the SEQ ID NO: 2; the first expression cassette is configured to increase the self-luminous intensity of a plant, plant tissue, or cell. According to the application, the LumEnhancer gene is further introduced on the basis of an FBP biological self-luminous system, so that the physiological activity of transgenic plant cells is effectively improved, and the energy metabolism level of the plant is further enhanced, thereby providing a more sufficient energy basis for a bioluminescence reaction and effectively enhancing the luminous intensity of the whole plant.

1. Name of the design product: bioreactor (2L). 2. Use of the design product: for plant cell expansion, cell therapy, antibody drug cell expansion. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view 1.

In various embodiments, provided herein are host cells, methods, and pharmaceutical compositions comprising gastrodin, wherein the gastrodin is produced by a genetically modified plant or plant cell, fungal cell, yeast cell, insect cell, or bacterial cell. In certain embodiments, the present disclosure provides methods and compositions for the production of gastrodin. In yet other embodiments, the present disclosure provides enhanced cells and methods for producing gastrodin.

The invention belongs to the technical field of gene engineering, and particularly relates to application of TuPK39 protein in stimulating plant defense reaction. The invention finds that the TuPK39 protein has a new application of stimulating plant defense reaction, and tests find that the TuPK39 protein can cause plant cell necrosis reaction, stimulate plant reactive oxygen bursting and stimulate expression of plant defense genes, and provides research basis and technical support for subsequent prevention and control research of pests including tetranychid mites.

The invention provides a composite material of plant cell membrane vesicles and cerium-based nano-enzyme. The composite material structurally comprises the plant cell membrane vesicles and the cerium-based nano-enzyme attached to the surfaces of the plant cell membrane vesicles, cerium ions of the cerium-based nano-enzyme and carboxyl groups on the surfaces of the plant cell membrane vesicles form coordination bonds; the plant cell membrane vesicles are from cruciferous plants, and carboxyl groups on the surfaces of the plant cell membrane vesicles comprise free carboxyl groups connected with the surfaces of the plant cell membrane vesicles through-NH-CO-PEGn-structures; the mass ratio of the cerium ions to the plant cell membrane protein is (0.1-0.5): 1. The invention also provides a method for preparing the composite material and application of the composite material in sepsis treatment. The composite material can specifically adsorb endotoxin and efficiently degrade the endotoxin, can be used for preventing / rescuing endotoxin-induced cell inflammation, and can also effectively relieve organ failure of sepsis model mice.

This invention discloses a method for determining the content of suberin monomers in the suberized tissue of yam tubers. Suberin is a macromolecular polyester, with monomers mainly covalently linked by oxygen-ester bonds. Its aliphatic compounds are linked to the polysaccharide components in the cell wall by glycosidic bonds and located on the inner side of the cell wall. Therefore, to determine the suberin monomer content, it is necessary to first destroy the plant cell structure, and then hydrolyze the oxygen-ester bonds between the suberin monomers to release the suberin monomer components. This invention first uses ultrasonic pretreatment to destroy plant cells, and then uses lipase hydrolysis combined with ultrasonic assistance and acid hydrolysis (sulfuric acid / methanol) to fully and efficiently hydrolyze the suberin into monomers. The reagents used are relatively safe and non-toxic, and the operation is relatively simple, solving the technical problem of difficult determination of suberin monomer content in the study of suberin metabolism after yam tuber injury healing.

The application discloses a kind of blending fertilizer raw material potash fertilizer and its preparation method, including the following weight parts of raw materials: 980 parts of potassium sulfate powder, 3 parts of zinc sulfate monohydrate, 10 parts of magnesium sulfate heptahydrate, 2 parts of sodium octaborate tetrahydrate, 1 part of DA-7, 4 parts of alginic polysaccharide.Seven water magnesium sulfate replenishes magnesium element, promotes the synthesis of carbohydrates, fats and proteins, is essential to maintain the green and healthy growth of crop leaves, and is conducive to improving the overall growth vigor and yield of crops.Four water sodium octaborate provides boron element, can promote pollen germination and pollen tube elongation, improve the flowering rate of crops, reduce the phenomenon of flower and fruit drop, DA-7 can promote plant cell division and elongation, improve the photosynthetic efficiency of plants, enhance the stress resistance and nutrient absorption capacity of plants, make crops grow more robustly, and improve fertilizer utilization.

The invention discloses a plant cell extract-containing traditional Chinese medicine aromatic penetration enhancer and a preparation method thereof, and the penetration enhancer comprises the following components in parts by mass: 8-15 parts of storax nano-vesicles, 3-8 parts of agilawood nano-vesicles, 1-5 parts of refined turpentine, 0.5-3 parts of panax notoginseng saponins, 0.5-3 parts of rhododendron dauricum oil and 20-35 parts of grape seed oil, the storax nano-vesicles and the agilawood nano-vesicles are exosome-like nano-vesicles extracted from plant secretion, and the particle size of the vesicles is 50-200 nm. Skin interstices are opened, skin meridians and blood are dredged, and accordingly relieving, inflammation resisting, redness improving and skin barrier repairing are achieved.

The invention relates to the technical field of biological medicines, in particular to a preparation method of protein cross-linked nano affinity microspheres, which comprises the following steps: preparing carboxylated silicon dioxide nano microspheres with the particle size of 500nm by adopting a sol-gel method, mixing tetraethoxysilane and ammonia water according to the volume ratio of 1: (3 + / -1), reacting in an ethanol solution at 40 DEG C for 6 hours, filtering, washing, and drying to obtain the protein cross-linked nano affinity microspheres. And centrifuging to obtain a microsphere precipitate, washing with deionized water for three times to finally obtain a nano microsphere suspension of which the surface is rich in carboxyl, and controlling the solid content to be 10 + / -0.5 mg / mL. The capturing efficiency of the finally prepared protein cross-linked nano affinity microspheres for exosomes reaches 95.0%, the binding capacity reaches 28.5 mu g / mg, the purity of the extracted exosomes is higher than that of an ultracentrifugation method, the biological activity and structural integrity of animal cell exosomes and plant cell exosomes are completely kept, and the protein cross-linked nano affinity microspheres are suitable for large-scale industrial production. The problems that in a traditional exosome extraction method, the recovery rate is low, impurity pollution is serious, exosome activity is damaged, and the application range is limited are solved.

The invention relates to the technical field of traditional Chinese medicine extraction and concentration, and discloses an efficient and energy-saving traditional Chinese medicine micro powder extraction and concentration system. Firstly, plant cell walls are fully destroyed through a traditional Chinese medicine micro-powder wall breaking technology, and complete and quantitative conveying of micro-powder materials is ensured in combination with an air pressure balance residue removing system which is arranged in a storage tank and composed of an air disc, an air blowing pipe and an air blowing plug. And secondly, direct contact type hot air heating and stirring are adopted in the extraction tank, and a two-stage dust suppression structure of spraying and wet dust removal is arranged, so that efficient extraction and dust control are realized. And finally, the system recycles the residual components of the decoction dregs through a secondary extraction process, and adopts a membrane filtration and membrane concentration technology to replace the traditional heating concentration, so that the filtration and concentration of the decoction are completed at normal temperature. The method effectively solves the problems of low extraction rate, high energy consumption, easy damage of thermosensitive components and dust pollution in the traditional process, and realizes high efficiency, energy conservation and high quality of production of the traditional Chinese medicine extraction preparation.

The invention discloses an application of an overexpressed CrOASTL1 gene in improving the soluble protein content of leguminous forage, and belongs to the technical field of biology. 1, and the CDS sequence of the CrOASTL1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: cloning a CrOASTL1 gene segment, constructing a plant overexpression vector, carrying out plant transgenosis operation, and introducing into leguminous forage plant cells to obtain a leguminous forage transgenosis line with the overexpressed CrOASTL1 gene. The CrOASTL1 gene is over-expressed in medicago sativa, and the soluble protein content of leaves of the medicago sativa is remarkably increased by about 19%. The CrOASTL1 gene of chlamydomonas reinhardtii is applied to quality improvement of leguminous forage for the first time, the technical obstacle that the genetic relationship of species is relatively far is overcome, a brand new gene resource and an efficient technical approach are provided for cultivating a new variety of leguminous forage with high protein and high nutritional value, and the method has important significance in promoting sustainable development of grass and animal husbandry.

Provided herein is a method of delivering a biological material, including a genome-editing agent, to the interior of a plant cell wherein the method includes contacting the plant cell with a complex comprising the biological material and a fluorous agent. Also provided herein are compositions and reagents for practicing the method.